CN120360925A - Composition for soothing skin and removing red blood streaks, and preparation method, application and product thereof - Google Patents
Composition for soothing skin and removing red blood streaks, and preparation method, application and product thereofInfo
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Abstract
本发明提供了一种舒缓肌肤、去除红血丝的组合物及制备方法、应用和产品，涉及化妆品技术领域。本发明的组合物按照重量份计，由以下组分组成：50‑200份欧洲越桔叶提取物、10‑50份棕榈酰茶提取物、2.5‑5份鞘氨醇单胞菌发酵产物提取物、32‑64份复合植物提取物A和0.12‑1.2份复合植物提取物B；复合植物提取物A为仙人掌提取物、麦冬根提取物、燕麦麸皮提取物、芍药根提取物和黄芩根提取物；复合植物提取物B为白薇提取物和积雪草提取物；欧洲越桔叶采用微波处理，再与复合酶进行酶解提取处理，得到欧洲越桔叶提取物。本发明的组合物具有显著去除红血丝、修复敏感肌肤的作用，能够应用于舒缓肌肤的化妆品的制备中。The present invention provides a composition for soothing skin and removing red blood streaks, and a preparation method, application and product, and relates to the field of cosmetic technology. The composition of the present invention is composed of the following components by weight: 50-200 parts of European bilberry leaf extract, 10-50 parts of palmitoyl tea extract, 2.5-5 parts of sphingomonas fermentation product extract, 32-64 parts of composite plant extract A and 0.12-1.2 parts of composite plant extract B; composite plant extract A is cactus extract, ophiopogon root extract, oat bran extract, peony root extract and scutellaria root extract; composite plant extract B is white lily extract and centella asiatica extract; European bilberry leaf is treated with microwaves, and then enzymatically extracted with a composite enzyme to obtain European bilberry leaf extract. The composition of the present invention has the effect of significantly removing red blood streaks and repairing sensitive skin, and can be applied to the preparation of cosmetics for soothing skin.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to a composition for relieving skin and removing red blood streaks, and a preparation method, application and a product thereof.
Background
The facial telangiectasis is commonly called as facial red blood vessel silk, and due to the fact that the elasticity of the facial telangiectasis tube wall is reduced, brittleness is enhanced, blood vessels are continuously and unevenly dilated or even ruptured, blood vessels are proliferated, so that facial skin redness and macroscopic dilated telangiectasis often occur, and red or mauve speckles, spots, lines or stars and other phenomena are accompanied. With the increase of age, the circulation speed of the microcirculation system is reduced, the number and quality of blood vessels are reduced, and vasodilation is accelerated, and the series of physiological changes lead to easy occurrence of red blood streaks on skin, on the other hand, after the skin barrier is damaged due to stimulation of factors such as physics, chemistry, hormone and the like, vascular Endothelial Growth Factor (VEGF) secreted by skin tissues causes vasodilation and increases vascular permeability, thereby leading to facial red blood streaks. When the skin is thin and sensitive, the face is redder when the emotion is excited and the temperature suddenly changes, and red blood streaks are easily caused in supercooled or overheated environments. The face of a patient with red blood streaks appears redder than the normal skin color, and the serious patient can form deposited color spots, thereby causing damage to the skin.
Red blood filaments also lead to the production of pro-inflammatory cytokines, the proliferation of matrix metalloproteinases and elastases, impaired skin barrier function, and excessive response to environmental stimuli, and decreased intercellular communication activity due to slow microcirculation and insufficient oxygen, resulting in increased skin aging rate.
In order to relax the skin and reduce red blood streaks of the skin, related cosmetics have been studied in the prior art. Chinese patent CN113827520A discloses a composition for removing red blood streak, its preparation method and application, the composition comprises 1-6 parts of coral algae extract, 1-5 parts of cornus officinalis extract, 0.1-10 parts of olive leaf extract, the above parts are by weight. The composition has effects in improving microcirculation, reducing vasodilation and vascular permeability, relieving inflammation, improving skin barrier, and preventing skin irritation.
Chinese patent No. 102000006A discloses a skin care composition with the effect of removing facial red blood streaks, which comprises a phase A and a phase B, wherein the phase A comprises water-soluble ceramide, VA palmitate and cholesterol stearate, and the phase B comprises a traditional Chinese medicine extract. The traditional Chinese medicine extract is prepared from rheum officinale, pagodatree flower bud, astragalus mongholicus and chamomile, and various components are reasonably combined, matched and shared by utilizing the principle of 'monarch, minister, assistant and guide' in the traditional Chinese medicine, so that the effect of removing facial red blood streaks is achieved.
Wang Haitao in the article "screening, extraction and action mechanism research of anti-sensitization and anti-irritation active substances for cosmetics", 25 plant extract is screened, and the result shows that the anti-sensitization and anti-irritation effects of cactus, dipotassium glycyrrhizinate, grape pip, oat alkaloid and mung bean fermentation products are obvious, and the anti-sensitization and anti-irritation effects of the cactus, grape pip and oat alkaloid have the effect of expanding the capillary blood vessels of a face all the time.
The existing composition with the effect of removing facial red blood streaks is mainly used for relieving skin, even the composition with the effects of moisturizing and moisturizing is used for repairing damaged skin, the effect of removing the red blood streaks is limited, and even the functional components contained in some cosmetics can aggravate the damage of the skin, so that the skin is more fragile. In view of the above, it is desired to provide a cosmetic composition which has clear functional components, and which can significantly remove red blood streaks and repair sensitive skin by the mutual cooperation of the components, and has no irritation to sensitive skin.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention provides a composition for relieving skin and removing red blood streaks, a preparation method, application and a product, wherein the composition comprises components of bilberry (VACCINIUM MYRTILLUS) leaf extract, palmitoyl tea extract, sphingomonas (SPHINGOMONAS) fermentation product extract, compound plant extract A and compound plant extract B which interact and cooperate, so that the effects of obviously removing red blood streaks, repairing sensitive skin, reducing VEGF secretion level, reducing histamine content, improving histamine release inhibition rate and reducing red pigment value are brought about, in addition, the bilberry leaf extract is prepared by adopting a microwave treatment and compound enzyme enzymolysis mode, and the content of active ingredients is high, thereby being beneficial to maintaining the integrity and stability of capillary vessels.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
Firstly, the invention provides a composition for relieving skin and removing red blood streaks, which comprises the following components, by weight, 50-200 parts of bilberry leaf extract, 10-50 parts of palmitoyl tea extract, 2.5-5 parts of Sphingomonas fermentation product extract, 32-64 parts of composite plant extract A and 0.12-1.2 parts of composite plant extract B;
the component of the composite plant extract A is cactus extract, radix ophiopogonis extract, oat bran extract, radix paeoniae alba extract and radix scutellariae extract;
the component of the composite plant extract B is cynanchum atratum extract and centella asiatica extract;
the preparation method of the bilberry leaf extract comprises the steps of carrying out microwave treatment on bilberry leaves, mixing with compound enzyme, and carrying out enzymolysis extraction treatment to obtain the bilberry leaf extract.
Preferably, the composition consists of, by weight, 80-150 parts of bilberry leaf extract, 20-40 parts of palmitoyl tea extract, 2.8-4 parts of Sphingomonas fermentation product extract, 42-54 parts of composite plant extract A and 0.5-0.8 part of composite plant extract B.
Further preferably, the composition consists of, in parts by weight, 100 parts of bilberry leaf extract, 30 parts of palmitoyl tea extract, 3 parts of Sphingomonas fermentation product extract, 48 parts of complex plant extract A and 0.6 part of complex plant extract B.
Preferably, in the compound plant extract A, the weight ratio of the cactus extract to the dwarf lilyturf tuber extract to the oat bran extract to the paeonia lactiflora root extract to the scutellaria baicalensis root extract is 14-28:6-12:6-12:4-8:2-4.
Further preferably, in the compound plant extract a, the weight ratio of the cactus extract, the ophiopogon root extract, the oat bran extract, the paeonia lactiflora root extract and the scutellaria baicalensis root extract is 21:9:9:6:3.
Preferably, in the composite plant extract B, the weight ratio of the cynanchum atratum extract to the centella asiatica extract is 0.1-1:0.02-0.2.
Further preferably, in the composite plant extract B, the weight ratio of the cynanchum atratum extract to the centella asiatica extract is 0.493-0.525:0.1.
Still more preferably, in the compound plant extract B, the weight ratio of cynanchum atratum extract to centella asiatica extract is 0.5:0.1.
Preferably, the preparation method of the bilberry leaf extract comprises the steps of mixing bilberry leaves with water in a solid-to-liquid ratio of 1:10-20 g/mL, carrying out microwave treatment for 90-150s at a microwave power of 250-400W, adding complex enzyme, and carrying out enzymolysis treatment at 40-50 ℃ for 18-36h to obtain the bilberry leaf extract.
Further preferably, the preparation method of the bilberry leaf extract comprises the steps of mixing bilberry leaves with water in a solid-to-liquid ratio of 1:15 g/mL, carrying out microwave treatment for 120s with microwave power at 320W, adding complex enzyme, and carrying out enzymolysis at 46 ℃ for 24h to obtain the bilberry leaf extract.
Further preferably, the complex enzyme is a cellulase and a pectinase in a weight ratio of 2:4-6.
Still more preferably, the complex enzyme is a 2:5 weight ratio of cellulase to pectase.
Further preferably, the amount of the complex enzyme is 0.01% -0.1% by weight of bilberry leaf.
Still more preferably, the complex enzyme is used in an amount of 0.07% to 0.1% by weight of bilberry leaf.
The invention also provides a preparation method of the composition, which comprises the step of uniformly mixing the components to obtain the composition.
Furthermore, the invention provides application of the composition in preparing cosmetics with effects of removing red blood streaks, relieving skin and repairing sensitive skin.
Finally, the invention provides a cosmetic with the effects of removing red blood streaks, relieving skin and repairing sensitive skin, which comprises the components of the composition.
Preferably, the cosmetic is a cosmetic having reduced VEGF secretion levels, reduced histamine levels, increased histamine release inhibition, red blood streak removal, reduced red pigment values.
Preferably, the cosmetic is not limited to face cream, toner, milky lotion, pack, makeup remover, cleansing lotion, face cleanser, essence, foundation, concealer, and sun block.
Preferably, the cosmetic ingredients further comprise moisturizers, emollients, emulsifiers, solvents, pH modifiers and thickeners.
Further preferably, the cosmetic ingredients may further include antioxidants, chelating agents, film forming agents and fragrances.
Further preferably, the humectant is a component conventionally used in the art, not limited to glycerin, polydimethylsiloxane, dimethiconol, betaine and 1, 2-hexanediol, and sodium hyaluronate.
Further preferably, the emollient is a component conventionally used in the art, not limited to ethylhexyl palmitate, petrolatum, caprylic/capric triglyceride, isononyl isononanoate and phenyl trimethicone.
Further preferably, the emulsifier is a component conventionally used in the art, not limited to cetostearyl alcohol, glyceryl stearate, PEG-100 stearate, polysorbate and potassium lauryl phosphate.
Further preferably, the solvent is a component conventionally used in the art, not limited to water, methyl propylene glycol, polyacrylamide, C 13-C14 isoparaffin, and laureth-7.
Further preferably, the pH adjustor is a component conventionally used in the art, not limited to arginine.
Further preferably, the thickener is a component conventionally used in the art, not limited to carbomers.
Further preferably, the antioxidant is a component conventionally used in the art, not limited to p-hydroxyacetophenone.
Further preferably, the chelating agent is a component conventionally used in the art, not limited to disodium EDTA.
Further preferably, the film former is a component conventionally used in the art, not limited to hydrogenated polyisobutene.
Compared with the prior art, the invention has the following beneficial effects:
1. in the composition, the bilberry leaf extract, the palmitoyl tea extract, the sphingomonas fermentation product extract, the compound extract A and the compound extract B interact and synergistically increase, so that the effects of efficiently removing red blood streaks and reducing the secretion level of Vascular Endothelial Growth Factor (VEGF) are brought, the effect of repairing sensitive skin of the composition is obvious, the protective capability of skin barriers can be obviously improved, and the percutaneous water loss is reduced.
2. The bilberry leaf extract in the composition is prepared by a microwave treatment and compound enzyme enzymolysis mode, the microwave treatment can accelerate leaching of active ingredients, the extraction temperature is mild, the structure and activity of the active ingredients are not damaged, the compound enzyme is adopted for mild enzymolysis treatment after the microwave treatment, the leaching of the active ingredients is further promoted, and the interaction between the prepared bilberry leaf extract and other ingredients brings excellent effects of repairing skin and removing red blood streaks.
Detailed Description
The following non-limiting examples will enable those of ordinary skill in the art to more fully understand the invention and are not intended to limit the invention in any way. The following is merely exemplary of the scope of the invention as it is claimed and many variations and modifications of the invention will be apparent to those skilled in the art in light of the disclosure, which should be considered as falling within the scope of the invention as claimed.
Where numerical ranges are provided in the examples, it is understood that unless otherwise stated herein, both endpoints of each numerical range and any number between the two endpoints are significant both in the numerical range. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
The invention is further illustrated by means of the following specific examples. The various chemical reagents and traditional Chinese medicine raw materials used in the embodiment of the invention are all obtained through conventional commercial approaches unless specified otherwise.
In the following examples, the palmitoyl tea extract was purchased from Bayer America, the Sphingomonas fermentation product extract was purchased from Xuezhou Waring biotechnology Co., ltd, the radix et caulis Opuntiae Dillenii extract, radix Ophiopogonis root extract, oat bran extract, radix Paeoniae root extract, radix Scutellariae root extract, radix Cynanchi Atrati extract and herba Centellae extract were all purchased from Magnolia officinalis (Guangzhou) technology development Co., ltd, the cellulase was purchased from Nanning Donghenghua biotechnology Co., ltd, the enzyme activity was 1 ten thousand U/g, the pectase was purchased from Nanning Donghenghua biotechnology Co., ltd, and the enzyme activity was 1 ten thousand-3 ten thousand U/g. Products of different manufacturers have no significant effect on the effect.
Example 1 to example 5
A composition for relieving skin and removing red blood streaks comprises the following components in parts by weight, specifically shown in Table 1.
TABLE 1
In the composite plant extract A of example 1-example 5, the weight ratio of the cactus extract, the radix Ophiopogonis extract, the oat bran extract, the radix Paeoniae extract and the radix Scutellariae extract is the same, and is 21:9:9:6:3.
The weight ratio of cynanchum atratum extract to centella asiatica extract in the composite plant extract a of example 1, example 4 and example 5 was 0.5:0.1, the weight ratio of cynanchum atratum extract to centella asiatica extract in the composite plant extract a of example 2 was 0.525:0.1, and the weight ratio of cynanchum atratum extract to centella asiatica extract in the composite plant extract a of example 3 was 0.493:0.1.
The bilberry leaf extract of example 1-example 5 was prepared by pulverizing bilberry leaves, mixing with water at a solid-to-liquid ratio of 1:15g:mL, treating with microwave power 320W for 120s, adding complex enzyme (cellulase and pectase) and performing enzymolysis at 46℃for 24h to obtain bilberry leaf extract, wherein the addition amount of complex enzyme is 0.07% of bilberry She Chongliang, and the weight ratio of cellulase and pectase is 2:5.
The components in Table 1 were mixed uniformly to obtain a composition.
Example 6
Unlike example 1, the preparation of bilberry leaf extract was different.
The preparation method of the bilberry leaf extract comprises pulverizing bilberry leaf, mixing with water at a solid-liquid ratio of 1:10g:mL, treating for 150s with microwave power of 250W, adding compound enzyme (cellulase and pectase) and performing enzymolysis at 50deg.C for 18h to obtain bilberry leaf extract, wherein the addition amount of the compound enzyme is 0.1% of bilberry She Chongliang, and the weight ratio of cellulase and pectase is 2:4.
The remainder was the same as in example 1.
Example 7
Unlike example 1, the preparation of bilberry leaf extract was different.
The preparation method of the bilberry leaf extract comprises pulverizing bilberry leaf, mixing with water at a solid-liquid ratio of 1:20g:mL, treating with microwave power 400W for 90s, adding compound enzyme (cellulase and pectase) and performing enzymolysis at 40deg.C for 36h to obtain bilberry leaf extract, wherein the addition amount of the compound enzyme is 0.1% of bilberry She Chongliang, and the weight ratio of cellulase and pectase is 2:6.
The remainder was the same as in example 1.
Comparative example 1
Unlike example 1, only palmitoyl tea extract, composite plant extract a and composite plant extract B components were contained in the composition.
The composition comprises, by weight, 30 parts of palmitoyl tea extract, 48 parts of a composite plant extract A and 0.6 part of a composite plant extract B, wherein the composite plant extract A comprises 21 parts of cactus extract, 9 parts of radix ophiopogonis root extract, 9 parts of oat bran extract, 6 parts of radix paeoniae alba root extract and 3 parts of radix scutellariae root extract, and the composite plant extract B comprises 0.5 part of cynanchum atratum extract and centella asiatica extract. The components are uniformly mixed to obtain the composition.
Comparative example 2
Unlike example 1, the composition contained only 2 components of bilberry leaf extract and Sphingomonas fermentation product extract.
The composition comprises, by weight, 100 parts of bilberry leaf extract and 3 parts of Sphingomonas fermentation product extract. The components are uniformly mixed to obtain the composition.
The preparation method of the bilberry leaf extract is the same as in example 1.
Comparative example 3
Unlike example 1, the Sphingomonas fermentation product extract was replaced with salicyl phytosphingosine (available from Heterograminis Biotechnology, CAS: 212908-67-3).
The composition comprises, by weight, 100 parts of bilberry leaf extract, 30 parts of palmitoyl tea extract, 3 parts of salicyl phytosphingosine, 48 parts of composite plant extract A and 0.6 part of composite plant extract B, wherein the composite plant extract A comprises 21 parts of cactus extract, 9 parts of radix ophiopogonis extract, 9 parts of oat bran extract, 6 parts of radix paeoniae alba extract and 3 parts of radix scutellariae extract, and the composite plant extract B comprises 0.5 part of radix cynanchi atrati extract and centella asiatica extract. The components are uniformly mixed to obtain the composition.
The preparation method of the bilberry leaf extract is the same as in example 1.
Comparative example 4
Unlike example 1, the bilberry leaf extract was replaced with the horse chestnut (Aesculus hippocastanum L) leaf extract.
The composition comprises, by weight, 100 parts of a horse chestnut leaf extract, 30 parts of a palmitoyl tea extract, 3 parts of a Sphingomonas fermentation product extract, 48 parts of a compound plant extract A and 0.6 part of a compound plant extract B, wherein the compound plant extract A comprises 21 parts of a cactus extract, 9 parts of a radix ophiopogonis root extract, 9 parts of an oat bran extract, 6 parts of a radix paeoniae alba root extract and 3 parts of a radix scutellariae root extract, and the compound plant extract B comprises 0.5 part of a cynanchum atratum extract and centella asiatica extract. The components are uniformly mixed to obtain the composition.
The preparation method of the European horse chestnut leaf extract comprises the steps of crushing European horse chestnut leaves, mixing the crushed European horse chestnut leaves with water at a solid-to-liquid ratio of 1:15 g/mL, treating the crushed European horse chestnut leaves for 120 seconds by adopting microwave power of 320W, and then adding compound enzyme (cellulase and pectase) to carry out enzymolysis treatment for 24 hours at 46 ℃ to obtain the European horse chestnut leaf extract, wherein the addition amount of the compound enzyme is 0.07% of European horse chestnut She Chongliang, and the weight ratio of the cellulase to the pectase is 2:5.
Comparative example 5
Unlike example 1, the components of the composite plant extract A were replaced with 21 parts of tea leaf extract, 9 parts of peony root extract, 9 parts of ginseng root extract, 6 parts of grape seed extract and 3 parts of paeonia root extract.
The preparation method of the extract comprises extracting folium Camelliae sinensis, radix moutan, radix Ginseng, grape seed and radix Paeoniae respectively with 8 times of water for 2 hr, concentrating the filtrate, and drying to obtain folium Camelliae sinensis extract, radix moutan extract, radix Ginseng extract, grape seed extract and radix Paeoniae extract.
The composition comprises, by weight, 100 parts of bilberry leaf extract, 30 parts of palmitoyl tea extract, 3 parts of Sphingomonas fermentation product extract, 48 parts of composite plant extract A and 0.6 part of composite plant extract B, wherein the composite plant extract A comprises 21 parts of tea tree leaf extract, 9 parts of peony root extract, 9 parts of ginseng root extract, 6 parts of grape seed extract and 3 parts of paeonia root extract, and the composite plant extract B comprises 0.5 part of cynanchum atratum extract and centella asiatica extract. The components are uniformly mixed to obtain the composition.
The preparation method of the bilberry leaf extract is the same as in example 1.
Comparative example 6
Unlike example 1, the composition has different proportions of the components in parts by weight.
The composition comprises, by weight, 20 parts of bilberry leaf extract, 100 parts of palmitoyl tea extract, 13 parts of Sphingomonas fermentation product extract, 0.6 part of composite plant extract A and 48 parts of composite plant extract B.
The remainder was the same as in example 1.
Comparative example 7
Unlike example 1, the bilberry leaf extract was prepared by a different method, and only one enzyme, pectase, was used for the enzymolysis.
The bilberry leaf extract is prepared by pulverizing bilberry leaf, mixing with water at a solid-to-liquid ratio of 1:15g:mL, treating with microwave power 320W for 120s, adding pectase of She Chongliang 0.07.07% of bilberry, and performing enzymolysis at 46 deg.C for 24 hr to obtain bilberry leaf extract.
The remainder was the same as in example 1.
Assay 1VEGF Activity assay
VEGF (vascular endothelial growth factor) activity was measured by ELISA kit. The detection steps are as follows:
(1) Blank group 100. Mu.L of Ham's F-12K medium containing 10% fetal bovine serum and Human Umbilical Vein Endothelial Cells (HUVEC) were inoculated in 96-well plates at a density of 1X 10 4 cells per well, cultured at 37 ℃ for 24 hours under 5% CO 2, then replaced with serum-free medium, cell culture supernatant was collected, and the secretion level of VEGF in HUVEC was detected by ELISA detection kit;
(2) A negative control group, which was inoculated with 100. Mu.L of Ham's F-12K medium containing 10% fetal bovine serum and Human Umbilical Vein Endothelial Cells (HUVEC) at a density of 1X 10 4 cells per well in a 96-well plate, cultured at 37℃for 24 hours under 5% CO 2, and then replaced with serum-free medium, irradiated with 15mJ/cm 2 UVB, and cultured for 24 hours, and cell culture supernatants were collected to examine the level of VEGF secretion in UVB-induced HUVECs;
(3) Experimental group 100. Mu.L of Ham' sF-12K medium containing 10% fetal bovine serum and Human Umbilical Vein Endothelial Cells (HUVEC) were inoculated in 96-well plates at a density of 1X 10 4 cells per well, cultured at 37℃for 24 hours in 5% CO 2, and then replaced with serum-free medium, 200. Mu.L of each composition solution was added (preparation of composition solution: 50mg of each composition was taken and 5mL of deionized water was added to dissolve completely to obtain composition solution), and the culture was continued for 24 hours by irradiation with 15mJ/cm 2 UVB, and cell culture supernatants were collected to measure the secretion level of VEGF in HUVEC.
The results of the VEGF secretion level (%), average.+ -. Standard deviation) assays for each group are shown in Table 2.
TABLE 2
In table 2, "/" indicates that there is no description of technical effects.
In Table 2, the negative control group had a significant difference (## P < 0.01) from the blank group, indicating that treatment with 15mJ/cm 2 UVB irradiation can increase VEGF activity, cause vasodilation and increase vascular permeability. The experimental group had a significant difference (△P＜0.05,△△ P < 0.01) compared to the negative control group, indicating that the composition had the technical effect of reducing UVB-induced VEGF secretion in HUVEC.
The comparative example group showed a significant difference (&P＜0.05,&& P < 0.01) from the example 1 group, indicating that the compositions of examples 1-7 were more effective than the comparative example group, and the compositions of examples had more excellent vascular dilation-reducing and VEGF secretion-inhibiting effects. Wherein the composition of comparative example 1 contains only palmitoyl tea extract, composite plant extract a and composite plant extract B, the composition of comparative example 2 contains only bilberry leaf extract and 2 components of Sphingomonas fermentation product extract, and the compositions of comparative example 1 and comparative example 2 can reduce VEGF activity, but the technical effects are weaker, and even if the VEGF activity reducing effects of comparative example 1 and comparative example 2 are added, the effects described in the examples cannot be achieved, so that the components of the composition of the present invention interact with each other, and the synergistic technical effects are brought about. Comparative example 4-comparative example 5 the replacement of some of the components in the composition with conventional soothing skin components, which effect is significantly different from that of the examples, which have a better effect of reducing VEGF secretion levels, indicates that the components in the composition of the present invention are not optionally replaced, that the choice of components is not conventional, and that the composition of the present invention brings unexpected technical effects. Comparative example 6 the effect of reducing the level of VEGF secretion by changing the weight ratio of each component in the composition was still inferior to that of comparative example 7 in which the preparation of bilberry leaf extract was performed using pectinase, the resulting composition was significantly reduced in the effect of reducing the level of VEGF secretion.
Test 2 Rat Basophil (RBL) histamine release test
Histamine is a substance released when cells are subjected to external stimulus and anaphylaxis, and the release of histamine leads to increased permeability of tissue wall, resulting in tissue edema and skin itching. The efficacy of the test compositions in relieving itching, soothing, repairing skin was assessed by measuring histamine release levels in RBL-2H3 cells.
The experimental method comprises collecting RBL-2H3 cells in logarithmic phase, inoculating 2.0-3.0X10 5 cells/mL/hole into 24-well plate, culturing in incubator for 18-24H, washing cells twice with HBSS buffer, adding DNP-IgE (immunoglobulin E) to the rest groups except blank control group to make its final concentration 1 μg/mL, and culturing in incubator for 24H to promote sufficient expression of high affinity receptor Fc epsilon RI on RBL-2H3 cell surface, washing cells twice with HBSS buffer, adding 100 μl of reagent, and using the following reagents:
The reagent for sample group is prepared by respectively taking 100mg of each composition, adding 5mL of DMSO for full dissolution to prepare a DMSO stock solution with the concentration of 2%, and then sequentially diluting to 1.0% by using a serum-free MEM culture medium;
the blank group uses a reagent, namely a serum-free MEM culture medium;
the model control group uses a reagent, namely a serum-free MEM culture medium;
3 wells per group were incubated for 1H, the cells were washed twice with HBSS, then HBSS (Hank's balanced salt solution) containing DNP-BSA at 0.5. Mu.g/mL was added to activate RBL-2H3 to release histamine, the reaction was stopped by ice bath for 10min after incubation for 1.5H, the supernatant was collected, stored at-80℃and the histamine content (ng/mL) was determined using ELISA kit.
The calculated formula of the histamine inhibition ratio is that the histamine inhibition ratio (%) = (histamine content M-histamine content TA)/(histamine content M-histamine content NT) ×100%.
The results of histamine content and histamine inhibition for each group are shown in Table 3.
TABLE 3 Table 3
In table 3, "/" indicates that there is no description of the effect of the related art.
In table 3, the model control group showed a significant difference in histamine content (## P < 0.01) compared to the blank group, indicating that the histamine release model was successfully prepared. The compositions of the examples of the present invention all significantly inhibited RBL-2H3 histamine release, the examples set had better inhibition than the comparative examples set, and the comparative examples each had significant differences in histamine inhibition (△△ P < 0.01) compared to example 1.
The comparison of the histamine inhibition rate data of comparative examples 1 and 2 and example 1 shows that the components in the composition of the present invention interact with each other to generate a synergistic effect, and the histamine inhibition rate data of comparative examples 3 and 4 and example 1 show that the effect of remarkably improving the histamine inhibition rate of the present invention cannot be achieved even if the components in the composition are replaced with other components with similar effects, and the technical effects of comparative examples 3 and 4 after the components are changed are remarkably reduced. Comparative example 6 the histamine inhibition rate was significantly reduced compared to example 1 after changing the weight ratios of the components of the composition, indicating that the specific ratios of the components in the composition provide significantly improved technical results.
Experiment 3 Red blood streak removal experiment
The compositions were prepared into creams according to the following steps:
(1) 7.5g ethylhexyl palmitate, 4.5g petrolatum, 3g cetostearyl alcohol, 3g caprylic/capric triglyceride, 3g isononyl isononanoate, 3g hydrogenated polyisobutene, 2g polydimethylsiloxane, 1.5g glyceryl stearate, 0.5g PEG-100 stearate, 2g polysorbate-60, 2g phenyl trimethicone, 1.6g polydimethylsiloxane and 0.4g polydimethylsiloxane alcohol, and stirring at 200rpm to raise the temperature to 80 ℃, stirring for 10min until the materials are uniform, thus obtaining phase A;
(2) 65g of water, 12.5g of glycerol, 2g of betaine, 0.45g of carbomer, 0.55g of nicotinamide, 0.55g of p-hydroxyacetophenone, 0.55g of potassium lauryl phosphate, 0.055g of sodium hyaluronate and 0.055g of disodium EDTA are mixed, stirred and heated to 80 ℃ at 200rpm, and stirred for 10min until the materials are uniform, so that a phase B is obtained;
(3) Stirring and mixing the phase A and the phase B at 400rpm, homogenizing and emulsifying for 10min, adding 2g of solvent (water, methyl propylene glycol, polyacrylamide, C 13-C14 isoparaffin and laureth-7 in a weight ratio of 42:35:15:8), and stirring at 400rpm for 10min until the mixture is uniform;
(4) Cooling to 45+ -3deg.C, adding 7.5g of methyl propylene glycol and 2g of the composition, stirring at 400rpm for 20min to mix well, packaging, and sterilizing to obtain facial cream containing each composition.
The compositions used in the preparation of the above-described creams were the composition of example 1, the composition of example 2, the composition of example 3, the composition of comparative example 1, the composition of comparative example 2 and the composition of comparative example 4, respectively.
42 Subjects were enrolled, healthy females aged 25-40 years, and had facial red blood streaks due to chemical skin irritation and some skin allergies, which were clinically manifested as continuous or intermittent reddening of the face, and the appearance of globose or filiform macroscopic red blood streaks on both sides of the cheekbones. The daily life of the subject does not need to be in the open air and is not exposed to sunlight frequently, the subject does not have serious chronic consumable diseases (such as asthma, diabetes and the like), skin lesions, inflammations, eczema and the like, the tested facial area does not have any scar, injury, uneven skin color, hair and other influence evaluation factors, the tested part is not contacted with skin treatment, cosmetology and other tests which possibly influence results for patients with serious system diseases, immunodeficiency or autoimmune diseases, the hormonal drugs and immunosuppressants are not used in the last 1 month, the tested part is not participated in other clinical testers in the current or last 3 months, and the test process can be understood, and the test is voluntarily participated in the test and the informed consent is signed.
Subject limitations are that the test site cannot use any other product with equivalent efficacy or that may have an effect on the test results during the test, that any cosmetic or therapeutic treatment of the test site during the test that would affect the test results is prohibited, and that the subject cannot change any product or change skin care habits during the test.
Skin melanin and heme test probeMX18, CK corporation, germany.
Subjects were randomized into 6 groups of 7 persons each. The compositions are respectively applied with the corresponding compositions, 1g each time, in the morning and evening, and can not be used with other skin care products during the test. After one month of continuous application, skin melanin and heme test probes were usedMX18 detects the red color value of the face (the portion where red blood streaks exist, the cheekbone) of the subject before and after applying the face cream, and records the average value of the red color values.
Effective rate = (red pigment value before application-red pigment value after application)/red pigment value before application x 100%.
The results of the detection of the haematochrome value and the results of the effective rate of each group of subjects are shown in Table 4.
TABLE 4 Table 4
| Face cream | Red pigment value before application | Post-application red pigment value | Effective rate (%) |
| Example 1 | 317.6 | 250.9 | 21.00 |
| Example 2 | 316.8 | 253.4 | 20.01 |
| Example 3 | 318.2 | 254.0 | 20.18 |
| Comparative example 1 | 316.0 | 288.3 | 8.77 |
| Comparative example 2 | 314.5 | 296.6 | 5.69 |
| Comparative example 4 | 319.4 | 276.5 | 13.43 |
As can be seen from Table 4, the composition of the example of the present invention can significantly reduce the skin haematochrome value, improve the skin haematochrome, and achieve the effect of soothing and repairing the skin, and the composition of the comparative example has significantly reduced effective rate of reducing haematochrome compared with the composition of the example, which indicates that the composition of the specific components of the present invention brings about significantly reduced effect of skin haematochrome.
Finally, it should be noted that the above description is only for illustrating the technical solution of the present invention, and not for limiting the scope of the present invention, and that the simple modification and equivalent substitution of the technical solution of the present invention can be made by those skilled in the art without departing from the spirit and scope of the technical solution of the present invention.
Claims (10)
1. A composition for soothing skin and removing red blood streaks is characterized by comprising, by weight, 50-200 parts of bilberry leaf extract, 10-50 parts of palmitoyl tea extract, 2.5-5 parts of Sphingomonas fermentation product extract, 32-64 parts of composite plant extract A and 0.12-1.2 parts of composite plant extract B;
the component of the composite plant extract A is cactus extract, radix ophiopogonis extract, oat bran extract, radix paeoniae alba extract and radix scutellariae extract;
the component of the composite plant extract B is cynanchum atratum extract and centella asiatica extract;
the preparation method of the bilberry leaf extract comprises the steps of carrying out microwave treatment on bilberry leaves, mixing with compound enzyme, and carrying out enzymolysis extraction treatment to obtain the bilberry leaf extract.
2. The composition according to claim 1, which comprises, by weight, 80-150 parts of bilberry leaf extract, 20-40 parts of palmitoyl tea extract, 2.8-4 parts of Sphingomonas fermentation product extract, 42-54 parts of complex plant extract A and 0.5-0.8 part of complex plant extract B.
3. The composition according to claim 2, which comprises, in parts by weight, 100 parts of bilberry leaf extract, 30 parts of palmitoyl tea extract, 3 parts of Sphingomonas fermentation product extract, 48 parts of complex plant extract A and 0.6 part of complex plant extract B.
4. A composition according to any one of claims 1-3, wherein the weight ratio of cactus extract, radix Ophiopogonis extract, oat bran extract, radix Paeoniae extract and radix Scutellariae extract in the complex plant extract a is 14-28:6-12:6-12:4-8:2-4.
5. A composition according to any one of claims 1 to 3, characterized in that the weight ratio of cynanchum atratum extract to centella asiatica extract in the complex plant extract B is 0.1-1:0.02-0.2.
6. A composition according to any one of claims 1 to 3, wherein the bilberry leaf extract is prepared by mixing bilberry leaf with water at a solid-to-liquid ratio of 1:10-20 g/mL, subjecting the mixture to microwave treatment at a microwave power of 250-400W for 90-150s, adding a complex enzyme, and subjecting the mixture to enzymolysis at 40-50 ℃ for 18-36 h.
7. The composition of claim 6, wherein the complex enzyme is a cellulase and pectase in a weight ratio of 2:4-6, and the complex enzyme is present in an amount of 0.01% to 0.1% by weight of the bilberry leaf.
8. The method for preparing the composition according to any one of claims 1 to 7, which comprises the step of uniformly mixing the components to obtain the composition.
9. Use of the composition according to any one of claims 1 to 7 for the preparation of a cosmetic having the efficacy of removing red blood streaks, soothing the skin, repairing sensitive skin.
10. A cosmetic having the effects of removing red blood streaks, soothing skin and repairing sensitive skin, comprising the composition of any one of claims 1 to 7.
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