CN204417516U - The special Micro-CPE neutralization test ware of laser scanning co-focusing microscope - Google Patents

Abstract

The utility model discloses a special micro cell culture dish for laser scanning confocal microscope, which comprises a culture dish body and an upper cover (7), wherein the culture dish body includes a shell (1) and is arranged inside the shell (1). A partition (2), the partition (2) divides the petri dish into a first chamber (5) and a second chamber (6) surrounding the outside of the first chamber (5), so The bottom of the first chamber (5) is a glass substrate (3), and the inner wall of the housing (1) is also provided with a scale line (4), and the height of the partition (2) is lower than the scale line (4). The micro-cell culture dish for laser scanning confocal microscope provided by the utility model has a simple structure and is especially suitable for the cultivation of precious micro-cells, which can make full use of the obtained cells, increase the cell density in the observable area of the culture dish, and reduce reagents. dosage, saving experiment cost.

Description

Micro cell culture dish for laser scanning confocal microscope

technical field

The utility model relates to the technical field of cell culture equipment in biomedical experiments, in particular to a special microcell culture dish for a laser scanning confocal microscope.

Background technique

Cells are the basic structural and functional units of all life activities. In biomedical research, cells are often cultured, and then cells are observed with a microscope for life science research. Laser scanning confocal microscopy is currently a more advanced cell biology Analytical instrument, which is equipped with a laser scanning device on the basis of fluorescence microscope imaging, and uses a computer for image processing, not only to observe fixed cells and tissue slices, but also to observe and detect the structure, molecules, and ions of living cells in real time and dynamically .

When observing living cells, it is necessary to use a special glass-bottom culture dish for laser scanning confocal microscopy. The bottom surface of the special glass-bottom petri dish for laser scanning confocal microscope is thin, the thickness of the glass bottom is 0.085-0.19mm, and the light transmission performance is good. One of the important supporting products of confocal microscope. Restricted by its structural characteristics, only the cells in the glass bottom area at the center of the bottom of the culture dish can be observed, while the cells in the plastic bottom area around the glass bottom area cannot be observed.

When the number of cells is large, as long as the number of cells is increased, enough cells can be observed on the glass bottom. However, in biomedical experiments, the number of cells obtained for biological research is often extremely small and extremely precious. If a small number of cells are added to a petri dish that is supported by a laser scanning confocal microscope for culture, the cells will be in the entire The bottom of the culture dish is scattered and attached to the wall, and in the experiment of observing cell culture by laser scanning confocal microscope, only the cells on the glass bottom can be used for experimental observation, so the conventional glass bottom culture dish for laser scanning confocal microscope is used for The culture of a small amount of rare cells makes the cell density observed by the laser scanning confocal microscope too low, which is not conducive to the observation of the experimental results.

Contents of the invention

The purpose of the utility model is to provide a special micro-cell culture dish for a laser scanning confocal microscope, which can make full use of the obtained cells, reduce cell waste, increase the density of cells in the observable area, reduce the amount of reagents, and save experimental costs.

Based on the above problems, the technical solution provided by the utility model is:

A microcell culture dish for laser scanning confocal microscope, comprising a culture dish body and an upper cover, the culture dish body includes a shell and a partition arranged inside the shell, the partition divides the culture dish into a first cavity chamber and a second chamber surrounding the first chamber, the bottom of the first chamber is a glass substrate, the inner wall of the housing is also provided with graduation marks, and the height of the partition is lower than The tick mark.

Further, the first chamber is located at the center and has a circular shape, and the second chamber is ring-shaped.

Further, the housing, the partition and the upper cover are made of medical plastic.

Compared with the prior art, the utility model has the advantages of:

1. Adopt the technical scheme of the present utility model, set the dividing plate inside the petri dish housing to divide the petri dish into a first chamber and a second chamber surrounding the outside of the first chamber, wherein the bottom of the first chamber is Glass bottom film, when using, place the cells to be cultured on the glass bottom film, then transfer the culture dish to the _CO2 incubator, after the cells adhere to the wall, add the culture solution to the scale line on the inner wall of the shell, and then proceed In the next step of the experiment, the observable cells only adhered to the glass bottom and did not scatter and adhere to the bottom of the entire culture dish, which increased the cell density in the observable area, especially for the small and precious cells, greatly increased its utilization.

2. By adopting the technical solution of the utility model, the number of cells required for experimental observation can be reduced, the waste of cells can be reduced, the cell density in the observable area can be increased, and the amount of experimental reagents can be reduced, thereby reducing the cost of the experiment.

Description of drawings

In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the following will briefly introduce the accompanying drawings that need to be used in the description of the embodiments. The accompanying drawings in the following description are only some embodiments of the utility model. Those of ordinary skill in the art can also obtain other drawings based on these drawings without any creative effort.

Fig. 1 is the structure schematic diagram of a kind of laser scanning confocal microscope dedicated micro cell culture dish embodiment of the utility model;

Fig. 2 is the top view of the petri dish body in the embodiment of the utility model;

Among them: 1. Shell; 2. Partition plate; 3. Glass negative film; 4. Graduation line; 5. First chamber; 6. Second chamber; 7. Top cover.

Detailed ways

The above solution will be further described below in conjunction with specific embodiments. It should be understood that these embodiments are used to illustrate the utility model and not limit the scope of the utility model. The implementation conditions used in the examples can be further adjusted according to the conditions of specific manufacturers, and the implementation conditions not indicated are usually the conditions in routine experiments.

Referring to Fig. 1-2, it is a structural schematic diagram of an embodiment of the present invention, providing a micro cell culture dish dedicated to a laser scanning confocal microscope, including a culture dish body and an upper cover 7, wherein the culture dish body includes a housing 1 and is arranged on A partition 2 in the housing 1, which divides the culture dish into a first chamber 5 and a second chamber 6 surrounding the outside of the first chamber 5, the bottom of the first chamber 5 is a glass negative 3 , the first chamber 5 is located in the center of the culture dish and is circular and the second chamber 6 is annular. The housing 1, the partition 2 and the upper cover 7 are all made of medical plastics. The housing 1 and the partition 2 can be It is integrally formed and the glass negative film 3 is bonded and fixed on the bottom of the casing 1 by medical grade shadowless glue.

A scale line 4 is also provided on the inner wall of the housing 1 , and the scale line 4 is used as the liquid level of the culture solution, and the height of the partition 2 is lower than the scale line 4 .

When in use, add 500ul culture solution to the first chamber, and pre-equilibrate in the incubator for 15 minutes. Aspirate the culture medium, drop the precious micro-cell hanging drop on the glass bottom of the first chamber, transfer the culture dish to the incubator, the cells only adhere to the glass bottom and will not disperse to the whole culture dish After the cells have settled and attached to the wall, add cell-free culture medium to the level of the scale line. This step can provide enough culture medium for the cells, and at the same time reduce the change of osmotic pressure caused by the volatilization of water. Then the next experiment can be carried out.

In summary, the micro-cell culture dish for laser scanning confocal microscope of the utility model can reduce the waste of cells, increase the density of cells in the observable area, greatly improve the utilization rate of cells, optimize the experimental operation steps, reduce the amount of reagents, and further Save experimental costs, especially suitable for the application of laser scanning confocal microscopy to observe a small number of precious cells.

The above examples are only to illustrate the technical concept and characteristics of the present utility model, and its purpose is to allow those familiar with this technology to understand the content of the present utility model and implement it accordingly, and cannot limit the protection scope of the present utility model with this. All equivalent transformations or modifications made according to the spirit of the utility model shall fall within the protection scope of the utility model.

Claims (3)

1. the special Micro-CPE neutralization test ware of laser scanning co-focusing microscope, comprise culture dish body and upper cover (7), it is characterized in that: described culture dish body comprises housing (1) and is arranged on the inner dividing plate (2) of described housing (1), described culture dish is divided into the first chamber (5) and is looped around outside the second chamber (6) of described first chamber (5) by described dividing plate (2), the bottom of described first chamber (5) is glass negative (3), the inwall of described housing (1) is also provided with scale marks (4), the height of described dividing plate (2) is lower than described scale marks (4).

2. the special Micro-CPE neutralization test ware of laser scanning co-focusing microscope according to claim 1, is characterized in that: described first chamber (5) is positioned at center and rounded, and described second chamber (6) ringwise.

3. the special Micro-CPE neutralization test ware of laser scanning co-focusing microscope according to claim 1, is characterized in that: described housing (1), described dividing plate (2) and described upper cover (7) adopt medical plastic to make.