CN203715615U - Modular alga photo-bioreactor - Google Patents

Abstract

The utility model discloses a modular algae photobioreactor. The algae cultivation standard module is connected with the illumination standard module through bolts, the other side of the algae cultivation standard module is connected with the ventilation standard module through bolts, and the algae cultivation standard module is connected with the cultivation standard module. The liquid measurement and control unit is connected by a hose, the first liquid inlet of the algae cultivation standard module is connected with the second liquid outlet of the culture medium measurement and control unit, and the liquid outlet of the algae cultivation standard module is connected with the second liquid inlet of the culture medium measurement and control unit. The ventilation standard module is connected to the gas measurement and control unit through a hose, the first air inlet of the ventilation standard module is connected to the second gas outlet of the culture fluid measurement and control unit, and the first gas outlet of the ventilation standard module is connected to the culture fluid measurement and control unit. The second liquid outlet of the unit is connected. The structure is simple, the operation is convenient, the stability of the equipment operation is improved, and the cultivation efficiency is increased by 5-8 times, which provides a good standardized experimental equipment platform for further optimizing the design and expanding the reaction volume.

Description

A Modular Algae Photobioreactor

technical field

The utility model belongs to the field of microalgae biotechnology, in particular to a high-efficiency modular algae photobioreactor, which utilizes the high-efficiency photosynthesis of algae and is suitable for high-efficiency production of algae biomass and CO ₂ in life support systems for space flight and submarine navigation. Fixation and O _regeneration .

Background technique

Since the beginning of human space manned exploration, the controlled ecological life support system has been paid attention to as the basis of manned spaceflight, and the _CO2 removal and _O2 regeneration involved in it is a key to the research of the controlled ecological life support system technology. Microalgae have become one of the important organisms in the study of controlled ecological life support systems due to their good adaptability, simple cultivation, stable operation, and efficient and rapid production of food and oxygen required by space crew. The growth of microalgae depends on Therefore, designing an efficient microalgae photobioreactor dedicated to a space-enclosed system is the key to conducting related research.

The design of the space microalgae photobioreactor should meet the following requirements: rapid growth of organisms in the reactor, high utilization rate of light energy, simple maintenance and control of the reactor, and effective separation of gas in liquid in a space microgravity environment. the

One of the key points in the design of space algae photobioreactor is to provide the culture conditions of high growth rate of algae, which needs to improve the technology of improving the utilization rate of light energy and strengthening the exchange of liquid substances while reducing the shear force. Improving light energy utilization can be achieved by increasing the ratio of illuminated surface area to volume, while enhancing liquid substance exchange while reducing shear force requires the selection of a stable and efficient circulation system, optimizing the flow of culture fluid and devices to achieve both. Balanced purpose. At present, photobioreactors dedicated to space mainly adopt airlift type (MELISSA, ESA) and plate type (BIOS-3, Russia), and also use horizontal reactor type (Guo Shuangsheng, etc.), but the main limitation of these reactors is the light The ratio of area to volume is small, so the light energy conversion efficiency is not high, and the flow of culture medium is unstable, which will generate strong shear force and affect the growth of cells. the

In addition, the choice of light source will also affect the efficiency of the reactor. Currently widely used light sources, such as sunlight, metal halide lamps, and cold incandescent lamps, have the disadvantages of high energy consumption, strong heat generation, and easy damage. Therefore, many LED-based reactors have been developed in recent years. The reactor is a light source, but it needs to be further optimized in terms of wavelength selection and ratio to adapt to the growth of algae. In addition, the standardization of the corresponding accessories of the LED light source is not high, and the existing light intensity measurement and control is relatively complicated, which needs further improvement. the

An important phenomenon in the space microgravity environment is that gas and liquid are not separated, so how to realize the gas-liquid separation under microgravity has always been one of the difficulties in the algae reactor. At present, most of the research is carried out by dynamic centrifugation, and hollow fiber membrane is also used. However, centrifugation depends on the centrifuge, which is unfavorable for space flight requirements in terms of power consumption and volume weight. The hollow fiber membrane method has low efficiency, It is easy to block and other problems, so it is urgent to develop a stable and efficient gas-liquid separation technology for space algae reactors. the

Traditionally designed reactors integrate functional devices such as cultivation, stirring, gas separation, and measurement and control as a whole. The design is complex, and each component cannot be operated independently or easily replaced, which greatly reduces the stability of the equipment and increases the complexity of operation. the

Contents of the invention

The purpose of this utility model is to provide a modular algae photobioreactor, which is simple in structure and easy to use. The utility model adopts a modular and standardized design idea, which can be used in algae cultivation, stirring, gas separation and measurement and control. Combination optimization can be achieved on the basis of each unit. Each unit can be operated independently or in combination, and can also be added or deleted according to actual convenience needs. Not only can the stability of equipment operation be greatly improved, and the cultivation efficiency can be increased by 5-8 times. It provides great convenience for maintenance and replacement, and provides a good standardized experimental equipment platform for further optimization of design and expansion of reaction volume. the

In order to achieve the above-mentioned purpose, the utility model adopts the following technical measures:

A modular algae photobioreactor, which includes a space algae photobioreactor consisting of an algae cultivation standard module, a lighting standard module, a ventilation standard module, a culture fluid measurement and control unit, and a gas measurement and control unit. It consists of an algae cultivation module, a lighting module , Breathable module; LED light source, Biofoil membrane, first air inlet, second air outlet, first liquid inlet, first liquid outlet, combination bolt, first peristaltic pump, second peristaltic pump, LED light source, photosensitive Battery, pH probe, dissolved oxygen temperature probe, conductivity probe, liquid change inlet, first liquid change outlet, second liquid inlet, second liquid outlet, second air intake, first air pump, oxygen measuring probe, carbon dioxide It consists of a measuring probe, a first ventilation inlet, a second ventilation outlet, a second air pump, and a second gas outlet.

The connection relationship is shown in Figure 1: the main body of the reactor is the algae cultivation standard module; the transparent side of the algae cultivation standard module is connected with the lighting standard module by bolts; and the other side of the algae cultivation standard module is also connected with the ventilation standard module by bolts The modules are connected; the algae cultivation standard module is connected with the culture fluid measurement and control unit through a hose, the connection relationship is that the first liquid inlet of the algae cultivation standard module is connected with the second liquid outlet of the culture fluid measurement and control unit, and the first outlet of the algae cultivation standard module is The liquid port is connected to the second liquid inlet of the culture fluid measurement and control unit; the ventilation standard module is connected to the gas measurement and control unit through a hose, and the connection relationship is the first air inlet of the ventilation standard module and the second outlet of the culture fluid measurement and control unit. The gas ports are connected, and the first gas outlet of the ventilation standard module is connected with the second liquid outlet of the culture solution measurement and control unit. the

The algae cultivation module 1 includes a combined standard reactor main body formed of transparent organic materials, a first liquid inlet, a first liquid outlet and fixing bolts on both sides. It is connected with the illumination standard module by fixing bolts on its light-transmitting side, and connected with the ventilation standard module by fixing bolts on the other side. The liquid in the algae cultivation standard module passes through the first liquid inlet, the first liquid outlet and the culture solution. The second liquid outlet and the second liquid inlet of the measurement and control unit are connected through soft rubber tubes; 4-8 groups of modular algae photobioreactors can be combined with bolts 10 according to needs, and the culture medium is processed by a multi-channel peristaltic pump. Exchange, to achieve high-efficiency mass culture. Compared with other integrated reactors, this module has the following advantages, that is, it realizes the function of the reactor and is easy to assemble and maintain. In addition, the reactor can be used for algae liquid and solid cultivation according to the cultivation needs, and the cross section can be designed as a rectangle. Or ellipse to ensure that the liquid flow does not leave a dead angle during culture, and it can also avoid the damage to the cells caused by the combined action of the fluid shear force and the culture container. In addition, because the designed lighting area is large, the ratio of the lighting area to the culture volume is also large, which significantly improves the utilization rate of light energy and increases the growth rate of algae cells to 5-8 times that of other reactors. the

The standard lighting module includes the standard accessories of the combined lighting system in the form of organic materials, multi-wavelength LED light sources and fixing bolts. The standard lighting module is connected to the light-transmitting surface of the algae cultivation standard module through bolts. The design advantage of this module is that it achieves specific functions and easy assembly and maintenance. At the same time, the lighting system is based on the single-color LED design of other reactors, and red, blue, and white LED light sources are added to it, which can be used for lighting. The ratio of color and light intensity can meet the needs of cultivating different algae materials. the

The standard ventilation module includes standard ventilation accessories in organic material configuration, Biofoil membrane system, first air inlet, first air outlet and fixing bolts. The module is connected to the side of the algae cultivation standard module through bolts, and its first air inlet and first air outlet are connected to the second air outlet and the second air inlet of the gas measurement and control component through rubber tubes. The design advantage of this module is that it realizes specific functions and easy assembly and maintenance. It can be assembled to the side of the reactor main body for gas-liquid separation in a space microgravity environment. The ventilation membrane is made of a special breathable and impermeable Biofoil membrane. composition. It can achieve high-efficiency separation of gas and liquid under microgravity, and at the same time avoid the complexity of centrifuge design and heavy weight and volume burden, and also avoid the disadvantages of low efficiency and easy blockage of hollow fiber tubes. the

The culture medium measurement and control unit includes the first peristaltic pump; the second peristaltic pump; LED light source; photosensitive cell; pH probe (pHG-2026B, Shanghai Kelan); conductivity probe (DDG-2022B, Shanghai Kelan); dissolved oxygen temperature probe (DOG-2028B, Shanghai Kelan); the first liquid change inlet; the second liquid change outlet; the second liquid inlet; the second liquid outlet. The culture solution measurement and control unit is connected to the algae cultivation module through a hose, and the second liquid inlet and the second liquid outlet are respectively connected to the first liquid outlet and the second liquid inlet of the algae cultivation module; when the culture solution flows in the system Determination of algae concentration (photosensitive cell to measure optical density), pH, conductivity and dissolved oxygen are respectively connected to the computer acquisition card, and the measurement and control data can be directly recorded by the computer; the first liquid change inlet and the second liquid change outlet are connected to the outside, Supplementation and replacement of culture medium and harvesting of algae; multi-channel peristaltic pump can control the degree of agitation by adjusting the flow rate. The design advantage of this component is that it realizes the external liquid measurement and control function, which ensures easy assembly and maintenance, and the peristaltic pump realizes the control of liquid flow and culture liquid entering and exiting the reactor. the

The gas measurement and control unit 5 includes the second air inlet; the second air outlet; the first air pump; the second air pump; the oxygen measuring probe (MIC-500-O2M, Shenzhen Yiyuntian); the carbon dioxide measuring probe (YTIAQ-CO2, Shenzhen Yiyuntian ); the first ventilation inlet; the second ventilation outlet, the gas measurement and control unit is connected to the ventilation standard module through a rubber tube, and the connection relationship is the second gas outlet, the second gas inlet and the ventilation The first air inlet and the second air outlet of the standard module. The design advantage of this part is that the gas measurement and control function is externally installed, which ensures easy assembly and maintenance. the

Compared with the prior art, the utility model has the following advantages and effects:

The utility model adopts the innovative design idea of standard modularization, and realizes the modular optimization design in the functions of algae cultivation, stirring, gas separation and measurement and control. It needs to be convenient to add and delete, which can not only greatly improve the stability of equipment operation, and increase the cultivation efficiency by 5-8 times, but also provide great convenience for later maintenance and replacement, and provide great support for further optimization of design and expansion of reaction volume. Good standardized experimental equipment platform. The main body of the algae reactor can be used for algae liquid and solid cultivation according to the cultivation needs. The cross-section can be designed as a rectangle or an ellipse to ensure that the liquid flow does not leave a dead angle during cultivation, and it can also avoid the damage to the cells caused by the fluid shear force and the cultivation container. harm. In addition, because the designed lighting area is large, the ratio of the lighting area to the culture volume is also large, which significantly improves the utilization rate of light energy and increases the growth rate of algae cells to 5-8 times that of other reactors. On the basis of the single-color LED design of other reactors, the standard lighting module adds red, blue and white LED light sources to it, and the ratio of light color and light intensity can be adjusted to meet the needs of different algae material cultivation. . The ventilation membrane of the standard ventilation module is composed of a special breathable and impermeable Biofoil membrane, which can realize the efficient separation of gas and liquid under microgravity, and at the same time avoid the complexity of centrifuge design and the heavy weight and volume burden, and also prevent the hollow The fiber tube has the disadvantages of low efficiency and easy clogging. The culture solution stirring and measurement and control components and the gas concentration measurement and control components realize the external liquid and gas measurement and control functions, ensuring easy assembly and maintenance, and efficient control of gas-liquid flow and replacement.

  Description of the drawings  

Figure 1 is a schematic diagram of the connection of a modular algae photoreactor.

Fig. 2 is a schematic side view of a modular photoreactor monomer. the

Fig. 3 is a schematic cross-sectional view of a single modular photoreactor. the

Figure 4 is a schematic diagram of the structure of a culture medium measurement and control unit. the

Fig. 5 is a schematic structural diagram of a gas measurement and control unit.

Figure 6 Combination diagram of four groups of modular algae photoreactors

Among them: 1. Algae cultivation module; 2. Illumination module; 3. Ventilation module; 4. Culture fluid measurement and control unit; 5. Gas measurement and control unit; 6. The first air inlet; 7. The second air outlet; 8. The first 9. The first liquid outlet; 10. Combined bolt; 11. The first LED light source; 12. Biofile film (NC0535503, produced by Fisher); 13. The first peristaltic pump; 14. The second peristaltic pump; 15. Second LED light source; 16. Photosensitive cell; 17. pH probe (pHG-2026B, Shanghai Kelan); 18. Conductivity probe (DDG-2022B, Shanghai Kelan); 19. Dissolved oxygen temperature probe (DOG- 2028B, Shanghai Kelan); 20. The first liquid change inlet; 21. The second liquid change outlet; 22. The second liquid inlet; 23. The second liquid outlet; 24. The second air inlet; 25. The second Two air outlets; 26. The first air pump; 27. The second air pump; 28. Oxygen measuring probe (MIC-500-O2M, Shenzhen Yiyuntian); 29. Carbon dioxide measuring probe (YTIAQ-CO2, Shenzhen Yiyuntian); 30. The first ventilation inlet; 31. The second ventilation outlet.

Detailed ways

Example 1:

According to Fig. 1, Fig. 2, Fig. 3, Fig. 4, Fig. 5, a kind of modular algae photobioreactor, it comprises space algae photobioreactor by algae cultivation standard module 1, illumination standard module 2, ventilation Standard module 3, culture medium measurement and control unit 4 and gas measurement and control unit 5. The connection relationship is shown in Figure 1: the main body of the reactor is the algae cultivation standard module 1; the transparent side of the algae cultivation standard module 1 is connected with the illumination standard module 2 through bolts 10; The bolt 10 is connected to the ventilation standard module 3; the algae cultivation standard module 1 is connected to the culture fluid measurement and control unit 4 through a hose, and the connection relationship is the first liquid inlet 8 of the algae cultivation standard module 1 and the second fluid inlet 8 of the culture fluid measurement and control unit 4. The liquid outlet 23 is connected, and the first liquid outlet 9 of the algae cultivation standard module 1 is connected with the second liquid inlet 22 of the culture solution measurement and control unit; the ventilation standard module 3 is connected with the gas measurement and control unit 5 through a hose, and the connection relationship is: The first gas inlet 6 of the gas standard module 3 is connected to the second gas outlet 24 of the culture fluid measurement and control unit, and the first gas outlet 7 of the ventilation standard module 3 is connected to the second gas inlet 23 of the culture fluid measurement and control unit 4 .

The algae cultivation standard module 1 (as shown in Fig. 2 and Fig. 3) includes a combined standard reactor body formed by transparent organic materials, a first liquid inlet 8, a first liquid outlet 9 and fixing bolts on both sides 10. Its light-passing side is connected with the illumination standard module 3 by fixing bolts 10, and on the other side of the illumination standard module 3, it is connected with the ventilation standard module 4 by fixing bolts, and the liquid in the algae cultivation standard module 1 passes through the liquid inlet 8 and the liquid outlet. The port 9 is connected to the second liquid outlet 23 and the second liquid inlet 22 of the culture medium measurement and control unit 4 through soft rubber tubes; 4-8 groups of modular algae photobioreactors can be combined with bolts 10 as required, and the The multi-channel peristaltic pump exchanges the culture medium to achieve a large amount of high-efficiency culture. the

The standard lighting module 2 (as shown in Figure 2) includes a combined lighting system standard accessory (lighting module main body) in an organic material configuration, a first LED light source 11 (multi-wavelength) and fixing bolts 10, the standard lighting module 2 and algae The light-transmitting surfaces of the cultivation standard modules 1 are connected by bolts 10 . The standard accessories of the combined lighting system and the first LED light source 11 are fixed by bolts 10 . the

The standard ventilation module 3 (as shown in FIG. 2 ) includes a standard ventilation fitting in an organic material configuration, a Biofoil membrane system, a first air inlet 6 , a first air outlet 7 and fixing bolts 10 . The standard ventilation module 3 is connected to the side of the algae cultivation standard module 1 by bolts 10, and its first air inlet 6 and first air outlet 7 are connected to the second air outlet 25 and the second air inlet of the gas measurement and control unit 5 through rubber tubes. Port 24 is connected. The standard ventilation fittings and the Biofoil membrane system are fixed by bolts 10. the

The culture solution measurement and control unit 4 (as shown in Figure 4) includes a first peristaltic pump 13.; a second peristaltic pump 14.; a second LED light source 15.; a photosensitive cell 16.; a pH probe (pHG-2026B, Shanghai Kelan ) 17.; conductivity probe (DDG-2022B, Shanghai Kelan) 18.; dissolved oxygen temperature probe (DOG-2028B, Shanghai Kelan) 19.; first liquid change inlet 20.; second liquid change outlet 21. ; The second liquid inlet 22.; The second liquid outlet 23.. The culture solution measurement and control unit is connected to the algae cultivation standard module 1 through a hose, and the second liquid inlet 22 and the second liquid outlet 23 are respectively connected to the first liquid outlet 9 and the first liquid inlet 8 of the algae cultivation standard module 1 , while the second liquid inlet 22 and the second liquid outlet 23 are respectively connected with the first peristaltic pump 13 and the second peristaltic pump 14 to control the flow of the culture solution; measure the current of the photosensitive cell 16 when the culture solution flows in the system ( reflect the concentration of algae liquid), pH probe 17, conductivity probe 18 and dissolved oxygen temperature probe 19 are respectively connected to the computer acquisition card, and the measurement and control data (including photosensitive cell 16, pH probe 17, conductivity probe 18 and dissolved oxygen temperature probe 19 ) can be directly recorded by the computer, and the second LED light source 15 controls the light through an external 220V power transformer; the first liquid change inlet 20 and the second liquid change outlet 21 are connected to the outside world through valves, and whether to supplement the culture liquid can be determined according to the concentration of the algae liquid , replacement and harvesting of algae; the multi-channel peristaltic pump can control the degree of stirring by adjusting the flow rate. the

The gas measurement and control unit 5 (as shown in Figure 5) includes a second air inlet 24.; a second air outlet 25.; a first air pump 26.; a second air pump 27.; an oxygen measuring probe (MIC-500-O2M , Shenzhen Yiyuntian) 28.; carbon dioxide measurement probe (YTIAQ-CO2, Shenzhen Yiyuntian) 29.; the first ventilation inlet 30.; the second ventilation outlet 31. The gas measurement and control unit 5 passes through the rubber tube and the ventilation The gas standard module 3 is connected, the gas measurement and control unit 5 is connected with the second gas outlet 25 and the second gas inlet 24 respectively, the ventilation standard module 3 is connected with the first gas inlet 6 and the first gas outlet 7, and the second gas outlet 25 is connected with the first air inlet 6, and the second air inlet 24 is connected with the first air outlet 7, and the second air inlet 24 and the second air outlet 25 are connected with the first air pump one 26 and the second air pump 27 respectively simultaneously. Connected to control the gas flow; when the gas flows in the system, the oxygen measuring probe 28 and the carbon dioxide measuring probe 29 are respectively connected to the computer acquisition card, and the measurement and control data can be directly recorded by the computer; the first ventilation inlet 30 and the second The ventilation outlet 31 is connected to the outside through a valve, and whether to connect to the outside can be selected according to the gas concentration measurement and control data in the system. the

Installation of multiple groups of reactors (as shown in Figure 6): 4 groups (respectively numbered A, B, C, D) of modular algae photobioreactors can be combined with bolts 10 as required, and the culture medium can be pumped by a multi-channel peristaltic pump. Perform measurement and control to achieve high-efficiency mass cultivation, and multiple sets of reactors can also be added as needed. the

Claims (6)

1. A modular algae photobioreactor, which includes an algae cultivation standard module (1), an illumination standard module (2), a ventilation standard module (3), a culture fluid measurement and control unit (4) and a gas measurement and control unit (5 ), characterized in that: the transparent side of the algae cultivation standard module (1) is connected to the lighting standard module (2) through bolts (10), and the other side of the algae cultivation standard module (1) is connected to the ventilation standard module through bolts (10) (3) connected, the algae cultivation standard module (1) is connected with the culture fluid measurement and control unit (4) through a hose, the first liquid inlet (8) of the algae cultivation standard module (1) is connected with the second outlet of the culture fluid measurement and control unit The liquid port (23) is connected, the first liquid outlet (9) of the algae cultivation standard module (1) is connected with the second liquid inlet (22) of the culture solution measurement and control unit (4), and the ventilation standard module (3) is connected with the The gas measurement and control unit (5) is connected through a hose, the first air inlet (6) of the ventilation standard module (3) is connected with the second gas outlet (24) of the culture solution measurement and control unit (4), and the ventilation standard module ( 3) The first gas outlet (7) is connected to the second liquid outlet (23) of the culture medium measurement and control unit (4). 2. A modular algae photobioreactor according to claim 1, characterized in that: the algae cultivation standard module (1) includes a combined standard reactor body formed by transparent organic materials, a first The liquid inlet (8), the first liquid outlet (9) and the fixing bolts (10) on both sides are connected with the lighting standard module (3) with the fixing bolts (10) on the light side, and the lighting standard module (3) ) and the other side is connected with the ventilation standard module (4) through fixing bolts, and the liquid in the algae cultivation standard module (1) passes through the first liquid inlet (8), the first liquid outlet (9) and the culture solution measurement and control unit ( 4) The second liquid outlet (23) and the second liquid inlet (22) are connected through a soft rubber tube. 3. A modular algae photobioreactor according to claim 1, characterized in that: said standard lighting module (2) includes standard accessories of combined lighting system in organic material configuration, multi-wavelength LED light source ( 11) and fixing bolts (10), the standard lighting module (2) is connected with the algae cultivation standard module (1) through the bolts (10), and the standard accessories of the combined lighting system are connected with the first LED light source (11) through the bolts ( 10) Fixed. 4. A modular algae photobioreactor according to claim 1, characterized in that: the standard ventilation module (3) includes standard ventilation accessories in organic material configuration, Biofoil membrane system, first The air inlet (6), the first air outlet (7) and the fixing bolt (10), the standard ventilation module (3) is connected to the side of the algae cultivation standard module (1) by bolts, the first air inlet (6), The first gas outlet (7) is connected to the second gas outlet (25) and the second gas inlet (24) of the gas measurement and control unit (5) through a rubber tube, and the standard ventilation accessories and the Biofoil membrane system are fixed by bolts (10) . 5. A modular algae photobioreactor according to claim 1, characterized in that: the culture solution measurement and control unit (4) includes a first peristaltic pump (13.), a second peristaltic pump (14. ), LED light source two (15), photosensitive cell (16), pH probe (17), conductivity probe (18), dissolved oxygen probe (19), first liquid change inlet (20), second liquid change outlet ( 21), the second liquid inlet (22), the second liquid outlet (23), the culture fluid measurement and control unit is connected to the algae cultivation standard module (1) through a hose, the second liquid inlet (22), the second outlet The liquid port (23) is respectively connected with the first liquid outlet (9) and the first liquid inlet (8) of the algae cultivation standard module (1), and the second liquid inlet (22) and the second liquid outlet (23 ) are respectively connected with the first peristaltic pump (13) and the second peristaltic pump (14), and the photosensitive cell (16), pH probe (17), conductivity probe (18) and dissolved oxygen temperature probe (19) are respectively connected with the computer to collect The card is connected, and the first liquid changing inlet (20) and the second liquid changing outlet (21) are connected with the outside through a valve. 6. A modular algae photobioreactor according to claim 1, characterized in that: the gas measurement and control unit (5) includes a second air inlet (24), a second air outlet (25), The first air pump (26), the second air pump (27), the oxygen measuring probe (28), the carbon dioxide measuring probe (29), the first ventilation inlet (30), the second ventilation outlet (31), gas The measurement and control unit (5) is connected to the ventilation standard module (3) through a rubber tube, the gas measurement and control unit (5) is respectively connected to the second gas outlet (25) and the second gas inlet (24), and the second gas outlet (25 ) is connected with the first air inlet (6), the second air inlet (24) is connected with the first air outlet (7), the second air inlet (24) and the second air outlet (25) are respectively connected with the first The air pump (26) is connected to the second air pump (27), the oxygen measuring probe (28) and the carbon dioxide measuring probe (29) are respectively connected to the computer acquisition card, the first ventilation inlet (30) and the second ventilation outlet (31) It is connected with the outside world through a valve.