CN223316687U - Portable small-sized fluorescent quantitative PCR instrument - Google Patents
Portable small-sized fluorescent quantitative PCR instrumentInfo
- Publication number
- CN223316687U CN223316687U CN202422469373.5U CN202422469373U CN223316687U CN 223316687 U CN223316687 U CN 223316687U CN 202422469373 U CN202422469373 U CN 202422469373U CN 223316687 U CN223316687 U CN 223316687U
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- CN
- China
- Prior art keywords
- sample block
- amplification
- heating
- quantitative pcr
- pcr instrument
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The utility model discloses a portable small fluorescent quantitative PCR instrument, which belongs to the field of medical instruments and comprises a cover body, a shell, a heating module and an amplification detection module, wherein the cover body is movably connected with the shell, the heating module is arranged on the cover body, the amplification detection module is arranged in the shell and comprises an amplification sample block, a refrigerating sheet, a radiating sheet and a fan, the amplification sample block is used for placing a PCR test tube, the amplification sample block and the radiating sheet are detachably connected with the fan, and the refrigerating sheet is clamped between the amplification sample block and the radiating sheet. The utility model has no moving parts in the interior, small volume, light weight, good damping effect, convenient carrying, low requirement on users, simple operation, automatic detection when the power switch is turned on, no additional operation, good heat dissipation effect, small heating sample block volume, high temperature circulation speed, short detection time and convenient rapid detection, and can simultaneously meet the requirements of professionals and families.
Description
Technical Field
The utility model relates to the technical field of medical appliances, in particular to a portable small fluorescent quantitative PCR instrument.
Background
The fluorescence quantification PCR (Polymerase Chain Reaction) technology is an important tool in the field of modern molecular biology, and is widely applied to aspects such as gene detection, pathogen diagnosis, gene expression analysis and the like. The traditional fluorescent quantitative PCR instrument is large in size, complex in structure and high in requirement on use environment. Such devices are generally suitable for laboratory environments, but their use is greatly limited in clinical field testing, field operations, and resource-constrained areas.
With the increasing popularity of molecular diagnostic techniques, there is an increasing market demand for portable, rapid and efficient fluorescent quantitative PCR instruments. The mainstream multichannel real-time fluorescence quantitative PCR instrument in the market is large in size, heavy in weight, complex in optical and mechanical systems and low in detection speed, and cannot realize on-site detection at any time and any place.
Disclosure of utility model
In order to overcome the defects of the prior art, the utility model aims to provide the portable small-sized fluorescent quantitative PCR instrument which is simple to operate, small in size, light in weight, high in detection speed, capable of improving the use efficiency and meeting the precision requirement, convenient for professionals to take out, and suitable for personal purchase for home self-inspection.
The utility model adopts the following technical scheme:
The utility model provides a portable small-size fluorescence ration PCR appearance, includes lid, casing, heating module, amplification detection module, the lid with casing swing joint, heating module sets up on the lid, the amplification detection module sets up in the casing, the amplification detection module includes amplification sample block, refrigeration piece, fin and fan, the amplification sample block is used for placing the PCR test tube, the amplification sample block the fin all with the fan can dismantle and be connected, the refrigeration piece press from both sides tightly in the amplification sample block with between the fin.
Further, the heating module comprises a heating sample block and a heating film, wherein the heating sample block is detachably connected with the cover body, and the heating film is stuck to the side face of the heating sample block.
Further, the heating film is made of polyimide flexible heating film.
Further, the heating sample block is provided with a mounting hole, and the mounting hole is used for mounting a PT100 platinum resistor attached with heat-conducting silica gel.
Further, the heating sample block is provided with a first through hole, an upper cover circuit board is arranged on the upper side of the heating sample block and used for controlling temperature and controlling light source to emit light, and the first through hole provides a light path for excitation light.
Further, the amplification sample block is provided with a test tube groove and a round hole, the test tube groove is positioned at the upper side and the lower side of the amplification sample block, the round hole is positioned at the side face of the test tube groove, and the round hole is used for transmitting emitted light and fixing the photodiode.
Further, the amplification sample block is further provided with a second through hole, the second through hole is located at two ends of the amplification sample block, and the second through hole is used for fixing the fluorescence detection circuit board.
Further, the cover body comprises a top cover and a bottom cover, and the top cover is detachably connected with the bottom cover.
Further, the shell comprises a shell body and a base, and the shell body is detachably connected with the base.
Further, the portable small-sized fluorescent quantitative PCR instrument further comprises a screen, wherein the screen is arranged on the shell and is in communication connection with the heating module and the amplification detection module.
Compared with the prior art, the portable small fluorescent quantitative PCR instrument comprises a cover body, a shell, a heating module and an amplification detection module, wherein the cover body is movably connected with the shell, the heating module is arranged on the cover body, the amplification detection module is arranged in the shell and comprises an amplification sample block, a refrigerating sheet, a radiating sheet and a fan, the amplification sample block is used for placing a PCR test tube, the amplification sample block and the radiating sheet are detachably connected with the fan, and the refrigerating sheet is clamped between the amplification sample block and the radiating sheet. The utility model has no moving parts in the interior, small volume, light weight, good damping effect, convenient carrying, low requirement on users, simple operation, automatic detection when the power switch is turned on, no additional operation, good heat dissipation effect, small heating sample block volume, high temperature circulation speed, short detection time and convenient rapid detection, and can simultaneously meet the requirements of professionals and families.
Drawings
FIG. 1 is a schematic diagram of the portable small-sized fluorescent quantitative PCR apparatus of the present utility model;
FIG. 2 is a schematic diagram of the heating module of the portable small-sized fluorescent quantitative PCR apparatus of FIG. 1;
FIG. 3 is a schematic diagram of the amplification detection module of the portable small-sized fluorescent quantitative PCR apparatus of FIG. 1.
10, A cover body, 11, a top cover, 12, a bottom cover, 20, a shell, 21, a shell body, 211, a heat dissipation hole, 22, a base, 221, an opening, 30, a heating module, 301, a heating sample block, 3011, a first through hole, 3012, a mounting hole, 302, a heating film, 40, an amplification detection module, 401, an amplification sample block, 4011, a test tube groove, 4012, a round hole, 4013, a second through hole, 402, a refrigerating sheet, 403, a heat dissipation sheet, 404, a fan, 50 and a screen.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
It will be understood that when an element is referred to as being "fixed to" another element, it can be directly on the other element or be present as another intermediate element through which the element is fixed. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. When an element is referred to as being "disposed on" another element, it can be directly on the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like are used herein for illustrative purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this utility model belongs. The terminology used herein in the description of the utility model is for the purpose of describing particular embodiments only and is not intended to be limiting of the utility model. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.
FIGS. 1-3 show a portable small-sized fluorescent quantitative PCR apparatus according to the present utility model, which includes a cover 10, a housing 20, a heating module 30, an amplification detection module 40, and a screen 50.
In this embodiment:
the cover body 10 comprises a top cover 11 and a bottom cover 12, the top cover 11 is fixedly connected with the bottom cover 12 through threads, and the bottom cover 12 is provided with a neodymium iron boron permanent magnet, so that the cover body 10 and the shell 20 are firmly and reliably closed during operation. In this embodiment, the cover 10 is mounted to the housing 20 by a hinge.
The shell 20 comprises a shell 21 and a base 22, the shell 21 is detachably and fixedly connected with the base 22, neodymium iron boron permanent magnets are arranged at positions, corresponding to the bottom cover 12, on the top of the upper side of the shell 21, the cover 10 and the shell 20 are firmly and reliably closed during operation, and heat dissipation holes 211 are formed in two sides of the shell 21.
The base 22 is provided with a threaded hole for fixing the main control board, and the side surfaces of the base are respectively provided with an opening 221 for fixing a power switch, a TYPEC communication interface and a DC power socket.
The heating module 30 comprises a heating sample block 301 and a heating film 302, wherein the heating sample block 301 is fixed on the side of the bottom cover 12 through bolts, the heating sample block 301 is provided with two first through holes 3011, the first through holes 3011 provide light paths for excitation light, and an upper cover circuit board is arranged on the upper side of the heating sample block 301 and used for controlling temperature and light source luminescence. The heating sample block 301 is also provided with a mounting hole 3012 with the diameter of 2mm and the depth of 10mm, and the mounting hole 3012 is convenient for placing a PT100 platinum resistor attached with heat conducting silica gel therein for temperature measurement.
A heating film 302 is adhered to the side of the heating block 301. In this embodiment, the heating film 302 is a polyimide flexible heating film 302.
The amplification detection module 40 is screwed to the upper side of the inside of the housing 21, and the amplification detection module 40 includes an amplification block 401, a cooling fin 402, a cooling fin 403, and a fan 404. In this embodiment, the amplification sample block 401, the cooling fin 402, the cooling fin 403 and the fan 404 are installed sequentially from top to bottom, and after the fan 404 is blown to the cooling fin 403 by the installation direction of the amplification detection module 40, the wind direction flow position is located at the position where the cooling holes 211 are opened at both sides of the housing 20.
The amplification block 401, the heat sink 403, and the fan 404 are connected by bolts. In this embodiment, the material of the amplification sample block 401 is aluminum alloy, and the surface of the aluminum alloy is anodized, so that the influence of an external light source on fluorescence detection is reduced.
Two test tube grooves 4011 with fixed depth are formed in the amplification sample block 401, round holes 4012 for conducting emitted light and fixing photodiodes are formed in the side face of the amplification sample block 401, and second through holes 4013 for fixing fluorescent detection circuit boards are formed in two ends of the amplification sample block 401.
The cooling plate 402 is coated with a layer of heat-conductive silicone grease and then clamped between the amplification block 401 and the heat sink 403. In this embodiment, the cooling plate 402 is made of a semiconductor material. Milk drink
The screen 50 is arranged on the shell 20 and is in communication connection with the heating module 30 and the amplification detection module 40, the screen 50 is fixed between the shell 21 and the base 22 through the inner buckles of the shell 21 and the base 22, and the base 22 is fixedly connected with the shell 21 through threads.
In the application, a cover body 10 is movably connected with a shell body 20, a heating module 30 is arranged on the cover body 10, an amplification detection module 40 is arranged in the shell body 20, and after a fan 404 blows to cooling fins 403 by the installation direction of the amplification detection module 40, the wind direction flowing position is positioned at the position of two sides of the shell body 20 and provided with cooling holes 211.
When the application is used, a sample and a reagent are added into a PCR test tube, the cover body 10 of the PCR instrument is opened, the test tube is put into the amplification detection module 40, the cover body 10 is closed, the PCR instrument is tightly closed under the action of the permanent magnets in the cover body 10 and the shell 20, the upper side of the PCR test tube is tightly attached to the heating module 30, the reagent is prevented from being condensed on the upper cover of the test tube in the temperature circulation process, a switch positioned on the base 22 is turned on, the screen 50 displays the temperature of the heating sample block 301, the temperature of the amplification sample block 401, the circulation times and the fluorescence detection result, the heating module 30 starts to work at first, the heating module is constant after being heated to a certain temperature, the amplification detection module 40 starts to work, the amplification sample block 401 circulates under the action of the refrigerating plate 402, the fan 404 does not work in the temperature circulation process, the fan 404 rotates at full speed when the temperature is reduced, the temperature circulation is finished, the fluorescence detection circuit works, and the amplification result is displayed on the screen 50. The application has no moving parts in the interior, small volume, light weight, good damping effect, convenient carrying, low requirement on users, simple operation, automatic detection when the power switch is turned on, no additional operation, good heat dissipation effect, small volume of the heating sample block 301, high temperature circulation speed, short detection time and convenient rapid detection, and can simultaneously meet the requirements of professionals and families.
The foregoing examples illustrate only a few embodiments of the utility model, which are described in detail and are not to be construed as limiting the scope of the utility model. It should be noted that, for those skilled in the art, it is possible to make several modifications and improvements without departing from the concept of the present utility model, which are equivalent to the above embodiments according to the essential technology of the present utility model, and these are all included in the protection scope of the present utility model.
Claims (10)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202422469373.5U CN223316687U (en) | 2024-10-12 | 2024-10-12 | Portable small-sized fluorescent quantitative PCR instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202422469373.5U CN223316687U (en) | 2024-10-12 | 2024-10-12 | Portable small-sized fluorescent quantitative PCR instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN223316687U true CN223316687U (en) | 2025-09-09 |
Family
ID=96933278
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202422469373.5U Active CN223316687U (en) | 2024-10-12 | 2024-10-12 | Portable small-sized fluorescent quantitative PCR instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN223316687U (en) |
-
2024
- 2024-10-12 CN CN202422469373.5U patent/CN223316687U/en active Active
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