DD263449A1 - PROCESS FOR THE OEKONOMIC AND SAFE HYGIENIZATION AND EXCLUSION INHIBITION OF MATERIALS AND BIOPRODUCTS - Google Patents

Abstract

The invention relates to a method for economical and gentle sanitation and germination inhibition of materials and organic products. The invention relates to an economical and gentle sanitation, sterilization or Auskeimungshemmung in the production of, for example Antigenpraeparationen, enzymes or foods, the sanitation of effluents, waste products or industrial materials, in the inhibition of germination of plant products or in Abtoetung animal systems. - This is achieved by adding biocidal / biostatic chemicals in lowest concentrations before or directly to the precursor or the later main stage for radiation-chemical reactions under the influence of ionizing radiation at low radiation doses or by chemical reactions taking place in the process arise. At the same time toxicological concerns are eliminated. Also, long-wave UV light or microwaves or ultrasound can be reduced together with underdosed chemicals.

Description

Field of application of the invention

The invention relates to a process for the economical and gentle sanitation and germ inhibition of materials and organic products used in the production of active substances, in the sanitation of foods or in waste water / waste products or industrial materials or in the germination of plant products or in the the killing of animal systems can be used.

Fields of application of the invention are the biotechnological and pharmaceutical industries, municipal hygiene, the food industry, agriculture and forestry, as well as companies that produce synthesis products.

Characteristic of the known technical solutions

The Hygierisierung or sterilization by ionizing radiation has been described versatile (DD-WP 142058, DD-WP153892, DD-WP 242636). Despite the many advantages that the radiation application brings in the aforementioned patent examples, the necessary radiation dose in a reasonable time unit is still very high and thus this way of industrial use too expensive, so that an introduction or application of radiation methods currently only some technologies can be realized.

Object of the invention

It is the object of the invention to achieve an economical and gentle sanitation or sterilization or germination inhibition multivalent in the most diverse materials and products or in many areas of application.

Explanation of the essence of the invention

The invention has for its object to develop a method to sanitize or sterilize with ionizing radiation at low to very low radiation doses, such as enzymes or antigen preparations, while preserving the specific activity, in the sterilization / germ reduction of food Inactivate corresponding foreign germs while largely preserving the nutritional value and the organoleptic properties to meet in the sanitation of wastewater / Abprodukten multivalent all germ groups or in other industrial application, for example in the form of clogged abrasives or films to inactivate the existing or developing germs or to inhibit their reproductive capacity.

According to the invention the object is achieved in that before or directly in the vOrstufe or the later main stage for radiation-chemical reactions under the influence of ionizing radiation added chemical compounds having biocidal / biostatic effect or produced by going in the process in front of chemical reactions, namely based on the chemical compounds or the effective element in concentrations below 150 g / kg or g / l and in chemical reactions below the Na> hweisgrenze.

Surprisingly, it has been found that the radiation doses thereby reduced to 5% to 95% of the otherwise required dose for inactivation / sterilization / Auskeimungshemmi.'ngen and thus the possible toxicological concerns in the range of 1OkGy from both the radiation and the respective chemicals can be eliminated.

Aüsführungsbeispielfi example 1

Parainfluenza viruses of type 3 are propagated on permanent monkey kidney cells. The virus-containing supernatant of the cell culture is mixed with a zwittarionic detergent (0.025% Empig '; i bl · Endki.n * eniration) k-jrz before irradiation. By gamma rays (cobalt-60: 2,5kGyk) the viruses are inactivated. The haagagg! Jtinalions Tito; (with 0.5% guinea pig erythrocytes), which before the irradiation is 1:32, remains unchanged.

Example 2

By centrifugation (max 2000gk) purified horse serum for microbiological purposes with a viable count (LkZ) of> 1.5 x 10 ¹⁰ / ml is irradiated in 11-glass bottles (with a tight seal) (radiation source = cobalt-60, max 18 ° C, dose rate / h = 8kGy).

Results:

20 kGy = sterile 10 kGy (+ addition of 100 ml HCHO: 20 hours before irradiation at 4 ⁰ C added) - sterile

1OkGy = u'isteriKL: ^: 1,6-1OVmI) Control - unsterile

example

An enzyme preparation (glycoamylase: 4450E / ml) is treated with a LKZ of 2.1 10 ⁸ / ml (1: 4000) and HCHO without irradiated (Kobait 60: 7,5kGy; 18 ⁰ C). After irradiation:

- With 'M,'. IO (addition 1 hour before): No germs detectable

- Without HCHO: 1,7-1O ³ LKZATiI

The enzyme activities after irradiation are in the range of 100 bir. 120%.

Example 4

Non-sterile and not surface-treated «! Scopyrol microplates are briefly fumigated with HCHO and immediately packed in polyethylene bags. The packaged test plates are exposed to cobalt 60 gamma rays (3 kGy). This sterility is achieved. At the same time, the binding capacity for antigens, antibodies and other reagents on the plates is increased.

Example 5

Candida utiüs (feed yeast), bred on molasses basis (sugar beet) and present in thick pulpy form, has been due to unfavorable breeding or environmental conditions with 3 · 10 ⁶ Salmonella a germs / ml - detectable-infected in culture media as living germs. The product is dickbreiige inch argen as follows irradiated (max 20 ⁰ C.): ELT-1,55kGy: 1.3 x Salrnonel a nuclei / ml 4kGy (5 hours before the addition of 0.5 g of sulfur dioxide / l): Salmonella Germs are no longer detectable.

Example 6

Broiler Gold (chicken) - are kontaminiert- with Salmonelia nuclei immersed in a solution (0.25 g of benzoic acid / I), then immediately sealed in polyethylene bags and with 3kGy irradiated (Cobalt 60.12 ⁰ C).

Result: No more living germs detectable.

Comparisons: control (without irradiation) and treated with 3kGy (without additive): live germs detectable.

Example 7

Sewage sludge with microbial flora (in concentrated form with> 10 ⁹ germs / ml) is irradiated! (Cobalt-60):

8kGy: Germ count reduction by 10Vml 4kGy (+ 0.2% chlorinated lime just before irradiation): No germ growth.

Example 8

Germinating potato tubers are briefly sprayed (0.75 g sulfur dioxide / l). With this treatment, 10 to 12Gy is enough to prevent germination.

Example 9

To pool water is added continuously chloramine (below 0.25% final concentration). Irradiation (cobalt-60: daowar output / h 3 to O, 5 kGy, initially higher, then declining) prevents germ multiplication. The same effect is achieved with other strongly under-dosed disinfectants.

Example 10

Grinding and cooling emulsions (for example for the manufacture of machine parts) are added dropwise (cobalt 60: 1 to eqGy) and simultaneously HCHO (in a final concentration up to 1: 1000). This prevents germ multiplication.

Example 11

Inactivation of Maui and Jaw Disease Viruses: A virus suspension is centrifuged at 10,000 g for about 25 min. The supernatant is recovered and treated with 1: 8000 HCHO. The irradiation takes place with 8 kGy (cobalt-60), whereby the viruses are inactivated. Further processing to an MKS adsorbate vaccine can be connected.

-4- 282

Example 12

A mixture of Klebsiella pneumoniae, Haemophilus influenzae, Pseudomonas aeruginosa, Escheyichia coli as well as a current strain of Streptococcus and a Diplococcus pneumoniae stamin will be equal to a concentration between 1-2 χ 10 ^a to 1-2 x 10 ¹⁰ germs / ml - Sharing mixed - brought. This mixture is mixed with HCHO (1: 12000K). - HCHO is below the Nachwois border. - Then irradiate: a) with Cobalt-60 (9kGy) or b) with microwaves under cooling (12s, 2450MHz, 1,5kW power output). There is inactivation. Either before or after inactivation, the membrane antigens can still be cleaved - depending on the application. This can also be done by enzymes. The mixture obtained can be administered both in the form of aerosol or as a liquid or lyophilisate or in enteric-coated form against the triggering of acute and chronic infections of the respiratory tract.

Claims (8)

A method for the economical and gentle sanitation and Auckeimungshornnuing of materials and ßioprodukten, characterized in that when using ionizing radiation on materials or products containing disturbing living or active germs, or living systems in general, the energy absorption, energy transfer and finally the energy effect by adding small amounts of chemical compounds with nontender or the growth or proliferation inhibitory effect, for example, special oxygen and Halogenabspalter, alcohols, adhesives, especially polyvalent, sometimes in combination with other active agents such as emulsifiers and surfactants or capillary Substances or compounds formed in the process by chemical reactions or catalysts, such as enzymes, possibly also in the presence or in conjunction with Radiolyseprodukten of water, the compounds also at the same time part chemically or totally, in part to produce new effects, can be activated, and at the same time an exaggerated synergistic effect occurs and through these targeted chemical additives, immediately before or up to 100 hours before the precursor or directly into the pre- / main stage for blasting -chemical reactions are made, which minimizes radiation doses. can be that in the case of the targeted production of active ingredients, such as microbial enzymes, they can be obtained in their activity i :: id still a sterilization or germ reduction can be achieved or in the S yygienisierung of foods nevertheless foreign germs, eg. Salmonella.n, are inactivated on receipt of the nutritional value and the organoleptic properties or effluents / waste trotaJem multivalent all germ groups in the sterilization effect were inactivated or in other industrial applications, such as in the form of industrial grit abrasives or special films, nevertheless all existing bacteria inactivated or greatly inhibited in their ability to multiply or in the case of irradiation of plant systems, eg. B. germinable potatoes, although a Auskeimungshemmung can be brought about or in the case of animal systems, eg. B. anobia, yet achieved a total kill and at the same time the toxicological concern by the radiation and by the chemical addition are eliminated. -2- 26? 449 Λ. Method according to points 1 to 3, characterized in that in Inaktivierungs-, Sterilisationsbzw. Auskeirhung & inhibition process together with the ionizing radiation, the concentration of the chemical compounds or the substances or the active element at concentrations between 150g / kg or, g / l to less than 0.0001 g / kg or g / l or due to the chemical reaction Below the detection limit and the radiation dose is reduced to 5 to 95% of the usual dose for inactivation / sterilization / Auskeimungshemmung. 2. The method according to item 1, characterized in that, depending on the respective applications or measures compounds or agents with algicides, amoebicides, askaricides, bactericidal / bacteriostatic, fungicidal / fungistatic, gametocides, germicides, helminthicides, herbicides, imagoziden, insecticides , larvicides, molluscicides, ryncozides / mycostatic, nematicides, ovicides, parasiticides, pesticides, protozides, rodenticides, schizontocides, sporicides, toxoplasmicides, trypanocides, vermicides, virucides / vii ostatic or cytocidal / cytostatic effects. 3. The method according to points 1 and 2, characterized in that for sanitation purposes, for example, aldehydes or mixtures of aldehydes, alkaline substances, alcohols, halogen compounds, lactones, metal compounds, oxidants, epoxides, phenols and xylenols or derivatives or mixtures, quaternary Ammonium compounds or mixtures or surfactants in general or the eiwähnten substances are used in mixtures with each other or for long-term preservative or In particular, for example, formic acid and its sodium, potassium and calcium compounds, benzoic acid, its sodium, potassium and calcium compounds, para-hydroxybenzoic acid ethyl ester, para-hydroxybenzoic acid npropyl ester and para-hydroxybenzoic acid methylesttr and their sodium compounds, propionic acid and their sodium -, potassium and calcium compounds, sorbic acid and its sodium, potassium and calcium compounds, calcium acetate, sulfur dioxide and sulfurous acid, sodium sulfite, calcium sulfite, sodium and Kaliumhydrogunsulfit, sodium and potassium disulfite, Sorboylpalmitat, oligodynamically active silver, hexamethylenetetramine and the corresponding Medium preparations calcium carbonate, citric acid, ethanol, acetic acid, glycerol, potassium carbonate, sodium chloride, lactic acid, sodium bicarbonate, sodium carbonate, tartaric acid or in mixtures with each other. 5. The method according to the items 1 and 4, characterized in that gamma rays or electron beams are used. 6. The method according to points 1 to 5, characterized in that in addition to an aqueous phase is also used in lyophilized or frozen, dry or gaseous state or in combination. 7. The method according to items 1,2,3,4 and 6, characterized in that, for example, long-wave UV light or microwaves or ultrasound are used in reduced use together with underdosed chemical compounds or elements. 8. The method according to the items 1 to 7, characterized in that existing or formed (radiation) protective substances are inactivated, so that the synergistic effect comes to full advantage.