DE10136008B4 - Verfahren zur Analyse von Makromolekülen und Verfahren zur Herstellung einer Analysevorrichtung - Google Patents

Verfahren zur Analyse von Makromolekülen und Verfahren zur Herstellung einer Analysevorrichtung Download PDF

Info

Publication number: DE10136008B4
Authority: DE; Germany
Prior art keywords: macromolecules; liquid; drop; spots; microarray
Prior art date: 2001-07-24
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Fee Related

Application number

DE10136008A

Other languages

German (de)

English (en)

Other versions

DE10136008A1 (de

Inventor

Jürgen Dr. Scriba

Christoph Dr. Gauer

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Beckman Coulter Inc

Original Assignee

Advalytix AG

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2001-07-24

Filing date

2001-07-24

Publication date

2005-03-31

2001-07-24 Application filed by Advalytix AG filed Critical Advalytix AG

2001-07-24 Priority to DE10136008A priority Critical patent/DE10136008B4/de

2002-07-09 Priority to PCT/EP2002/007644 priority patent/WO2003012389A2/fr

2002-07-09 Priority to EP02764659A priority patent/EP1412533A2/fr

2002-07-09 Priority to AU2002328867A priority patent/AU2002328867A1/en

2003-02-20 Publication of DE10136008A1 publication Critical patent/DE10136008A1/de

2005-03-31 Application granted granted Critical

2005-03-31 Publication of DE10136008B4 publication Critical patent/DE10136008B4/de

2021-07-25 Anticipated expiration legal-status Critical

Status Expired - Fee Related legal-status Critical Current

Links

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JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 4
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Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
- B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00497—Features relating to the solid phase supports
- B01J2219/00527—Sheets
- B01J2219/00533—Sheets essentially rectangular
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
- B01J2219/00659—Two-dimensional arrays
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/0068—Means for controlling the apparatus of the process
- B01J2219/00702—Processes involving means for analysing and characterising the products
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0636—Integrated biosensor, microarrays
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/089—Virtual walls for guiding liquids
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
- B01L2400/0436—Moving fluids with specific forces or mechanical means specific forces vibrational forces acoustic forces, e.g. surface acoustic waves [SAW]
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0433—Moving fluids with specific forces or mechanical means specific forces vibrational forces
- B01L2400/0439—Moving fluids with specific forces or mechanical means specific forces vibrational forces ultrasonic vibrations, vibrating piezo elements
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0493—Specific techniques used
- B01L2400/0496—Travelling waves, e.g. in combination with electrical or acoustic forces
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Immunology (AREA)
Life Sciences & Earth Sciences (AREA)
Hematology (AREA)
Engineering & Computer Science (AREA)
Dispersion Chemistry (AREA)
Analytical Chemistry (AREA)
General Health & Medical Sciences (AREA)
Urology & Nephrology (AREA)
Molecular Biology (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
Cell Biology (AREA)
Microbiology (AREA)
Chemical Kinetics & Catalysis (AREA)
Clinical Laboratory Science (AREA)
Food Science & Technology (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Biochemistry (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Investigating Or Analyzing Materials By The Use Of Ultrasonic Waves (AREA)

DE10136008A 2001-07-24 2001-07-24 Verfahren zur Analyse von Makromolekülen und Verfahren zur Herstellung einer Analysevorrichtung Expired - Fee Related DE10136008B4 (de)

Priority Applications (4)

Application Number	Priority Date	Filing Date	Title
DE10136008A DE10136008B4 (de)	2001-07-24	2001-07-24	Verfahren zur Analyse von Makromolekülen und Verfahren zur Herstellung einer Analysevorrichtung
PCT/EP2002/007644 WO2003012389A2 (fr)	2001-07-24	2002-07-09	Procede pour analyser des macromolecules, dispositif d'analyse et procede pour produire un dispositif d'analyse
EP02764659A EP1412533A2 (fr)	2001-07-24	2002-07-09	Procede pour analyser des macromolecules, dispositif d'analyse et procede pour produire un dispositif d'analyse
AU2002328867A AU2002328867A1 (en)	2001-07-24	2002-07-09	Method for analysing macromolecules, analysis device and a method for producing an analysis device

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
DE10136008A DE10136008B4 (de)	2001-07-24	2001-07-24	Verfahren zur Analyse von Makromolekülen und Verfahren zur Herstellung einer Analysevorrichtung

Publications (2)

Publication Number	Publication Date
DE10136008A1 DE10136008A1 (de)	2003-02-20
DE10136008B4 true DE10136008B4 (de)	2005-03-31

Family

ID=7692898

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DE10136008A Expired - Fee Related DE10136008B4 (de)	2001-07-24	2001-07-24	Verfahren zur Analyse von Makromolekülen und Verfahren zur Herstellung einer Analysevorrichtung

Country Status (4)

Country	Link
EP (1)	EP1412533A2 (fr)
AU (1)	AU2002328867A1 (fr)
DE (1)	DE10136008B4 (fr)
WO (1)	WO2003012389A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE10328624A1 (de) *	2003-06-25	2005-01-20	Advalytix Ag	Verfahren zur Hybridisierung
DE102004037348A1 (de) *	2004-08-02	2006-03-16	Infineon Technologies Ag	Fluid-Transport-Vorrichtung, Sensor-Anordnung, Fluid-Misch-Vorrichtung und Verfahren zum Herstellen einer Fluid-Transport-Vorrichtung
DE102004051394B4 (de) *	2004-10-21	2006-08-17	Advalytix Ag	Verfahren zur Bewegung von kleinen Flüssigkeitsmengen in Mikrokanälen und Mikrokanalsystem
DE102005000835B3 (de)	2005-01-05	2006-09-07	Advalytix Ag	Verfahren und Vorrichtung zur Dosierung kleiner Flüssigkeitsmengen
EP1833598B1 (fr)	2005-01-05	2008-10-08	Olympus Life Science Research Europa GmbH	Procede et dispositif de dosage et de melange de petites quantites de liquide
DE102005056639A1 (de) *	2005-11-28	2007-06-06	Advalytix Ag	Verfahren, Vorrichtung und Kit zur Untersuchung von Makromolekülen in einer Probe
DE102006056694B4 (de) *	2006-11-30	2010-08-05	Advalytix Ag	Verfahren zum Durchführen einer enzymatischen Reaktion
WO2008080531A2 (fr) *	2007-01-05	2008-07-10	Advalytix Ag	Procédé, dispositif et kit pour l'examen d'un échantillon de liquide
EP2194381B1 (fr)	2008-12-03	2015-12-02	Roche Diagnostics GmbH	Elément de test doté d'une zone de contrôle et de calibrage combinée
WO2013116311A1 (fr) *	2012-01-31	2013-08-08	The Penn State Research Foundation	Manipulation microfluidique et tri de particules à l'aide d'une onde acoustique de surface stationnaire et synchronisable

Citations (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO1994005414A1 (fr) *	1992-08-31	1994-03-17	The Regents Of The University Of California	Reacteur microfabrique
JPH0927590A (ja) *	1995-07-10	1997-01-28	Nippon Inter Electronics Corp	複合半導体装置
US5653939A (en) *	1991-11-19	1997-08-05	Massachusetts Institute Of Technology	Optical and electrical methods and apparatus for molecule detection
DE19827900A1 (de) *	1997-10-21	1999-04-22	Roche Diagnostics Gmbh	Verfahren zur Beschichtung von Oberflächen
WO2000010011A1 (fr) *	1998-08-10	2000-02-24	Biotul Ag	Dispositif et procede pour melanger a proximite de l'interface des echantillons dans des systemes biocapteurs
WO2000016082A1 (fr) *	1998-09-16	2000-03-23	Commissariat A L'energie Atomique	Dispositif comprenant une pluralite de sites d'analyse sur un support
DE19940752A1 (de) *	1998-08-28	2000-04-27	Febit Ferrarius Biotech Gmbh	Verfahren zur Herstellung eines mit biologisch oder chemisch funktionellen Materialien beschichteten Trägers (BioChip)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5474796A (en) *	1991-09-04	1995-12-12	Protogene Laboratories, Inc.	Method and apparatus for conducting an array of chemical reactions on a support surface
US6010316A (en) *	1996-01-16	2000-01-04	The Board Of Trustees Of The Leland Stanford Junior University	Acoustic micropump

2001
- 2001-07-24 DE DE10136008A patent/DE10136008B4/de not_active Expired - Fee Related
2002
- 2002-07-09 WO PCT/EP2002/007644 patent/WO2003012389A2/fr not_active Application Discontinuation
- 2002-07-09 EP EP02764659A patent/EP1412533A2/fr not_active Withdrawn
- 2002-07-09 AU AU2002328867A patent/AU2002328867A1/en not_active Abandoned

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5653939A (en) *	1991-11-19	1997-08-05	Massachusetts Institute Of Technology	Optical and electrical methods and apparatus for molecule detection
WO1994005414A1 (fr) *	1992-08-31	1994-03-17	The Regents Of The University Of California	Reacteur microfabrique
JPH0927590A (ja) *	1995-07-10	1997-01-28	Nippon Inter Electronics Corp	複合半導体装置
DE19827900A1 (de) *	1997-10-21	1999-04-22	Roche Diagnostics Gmbh	Verfahren zur Beschichtung von Oberflächen
WO2000010011A1 (fr) *	1998-08-10	2000-02-24	Biotul Ag	Dispositif et procede pour melanger a proximite de l'interface des echantillons dans des systemes biocapteurs
DE19940752A1 (de) *	1998-08-28	2000-04-27	Febit Ferrarius Biotech Gmbh	Verfahren zur Herstellung eines mit biologisch oder chemisch funktionellen Materialien beschichteten Trägers (BioChip)
WO2000016082A1 (fr) *	1998-09-16	2000-03-23	Commissariat A L'energie Atomique	Dispositif comprenant une pluralite de sites d'analyse sur un support

Also Published As

Publication number	Publication date
WO2003012389A2 (fr)	2003-02-13
EP1412533A2 (fr)	2004-04-28
AU2002328867A1 (en)	2003-02-17
DE10136008A1 (de)	2003-02-20
WO2003012389A3 (fr)	2003-07-17

Publication	Publication Date	Title
EP1451586B1 (fr)	2007-01-24	Procede et dispositif pour determiner la concentration de ligands contenus dans un echantillon a analyser
DE60010666T2 (de)	2005-05-19	Verfahren und vorrichtung zur programmierbaren behandlung von fluiden
DE10164357B4 (de)	2005-11-10	Titrationsverfahren
WO1999027367A1 (fr)	1999-06-03	Dispositif et procede permettant de detecter des substances a analyser
EP1277055B1 (fr)	2008-04-30	Biopuce pour l'archivage et l'analyse en laboratoire medical d'un echantillon biologique
EP1409722B1 (fr)	2009-03-25	Procede d'analyse de macromolecules
DE10136008B4 (de)	2005-03-31	Verfahren zur Analyse von Makromolekülen und Verfahren zur Herstellung einer Analysevorrichtung
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DE10120035B4 (de)	2005-07-07	Verfahren und Vorrichtung zur Manipulation kleiner Flüssigkeitsmengen auf Oberflächen
EP0905515B1 (fr)	2004-12-08	Méthode pour la mésure analytique et son usage
DE69716638T2 (de)	2003-08-14	Oberflächenmuster von affinitätreagentien mittels photoablation
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EP2414821B1 (fr)	2013-08-14	Dispositif du type caméra électrochimique, ainsi que procédé de fabrication, et utilisation du dispositif
DE10164358C2 (de)	2003-11-27	Charakterisierungsverfahren für funktionalisierte Oberflächen
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DE10117771A1 (de)	2002-10-17	Verfahren und Vorrichtung zur Manipulation kleiner Flüssigkeitsmengen und/oder darin enthaltener Teilchen
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EP1811300B1 (fr)	2008-11-12	Puce de capteur avec des récepteurs inclus dans un réseau polymérique
DE10307801A1 (de)	2004-09-09	Analyseverfahren und -device zur Untersuchung von spezifischen Bindungsereignissen
DE10125020A1 (de)	2002-12-05	Chip-Anordnung und Verfahren zum Immobilisieren von Fängermolekülen auf einer Sensorelektrode unter Verwendung der Chip-Anordnung
DE10117866A1 (de)	2002-10-17	Verfahren und Vorrichtung zur Charakterisierung und/oder zum Nachweis eines Bindungskomplexes
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