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DE68922743D1 - Verfahren zur herstellung von cdns. - Google Patents

Verfahren zur herstellung von cdns.

Info

Publication number
DE68922743D1
DE68922743D1 DE68922743T DE68922743T DE68922743D1 DE 68922743 D1 DE68922743 D1 DE 68922743D1 DE 68922743 T DE68922743 T DE 68922743T DE 68922743 T DE68922743 T DE 68922743T DE 68922743 D1 DE68922743 D1 DE 68922743D1
Authority
DE
Germany
Prior art keywords
mrna
support
dna
cdns
producing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE68922743T
Other languages
English (en)
Other versions
DE68922743T2 (de
Inventor
Erik Hornes
Lars Korsnes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies AS
Original Assignee
Dynal AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB888827158A external-priority patent/GB8827158D0/en
Priority claimed from GB888827159A external-priority patent/GB8827159D0/en
Application filed by Dynal AS filed Critical Dynal AS
Publication of DE68922743D1 publication Critical patent/DE68922743D1/de
Application granted granted Critical
Publication of DE68922743T2 publication Critical patent/DE68922743T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Luminescent Compositions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
DE68922743T 1988-11-21 1989-11-21 Verfahren zur herstellung von cdns. Expired - Lifetime DE68922743T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB888827158A GB8827158D0 (en) 1988-11-21 1988-11-21 Process for producing cdna
GB888827159A GB8827159D0 (en) 1988-11-21 1988-11-21 Subtraction cdna library construction
PCT/EP1989/001418 WO1990006044A2 (en) 1988-11-21 1989-11-21 PROCESS FOR PRODUCING cDNA

Publications (2)

Publication Number Publication Date
DE68922743D1 true DE68922743D1 (de) 1995-06-22
DE68922743T2 DE68922743T2 (de) 1995-10-26

Family

ID=26294647

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68922743T Expired - Lifetime DE68922743T2 (de) 1988-11-21 1989-11-21 Verfahren zur herstellung von cdns.

Country Status (6)

Country Link
EP (1) EP0444119B1 (de)
JP (1) JP2960776B2 (de)
AT (1) ATE122721T1 (de)
CA (1) CA2003500C (de)
DE (1) DE68922743T2 (de)
WO (1) WO1990006044A2 (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
EP0533952A4 (en) * 1991-04-15 1993-07-07 Sumitomo Electric Industries, Ltd. Process for separating or extracting nucleic acid from specimen containing the same
US6316182B1 (en) * 1992-01-15 2001-11-13 Nen Life Science Products, Inc. Detection of reverse transcriptase by DNA hybridization assay
WO1993015228A1 (en) * 1992-01-29 1993-08-05 Hitachi Chemical Co., Ltd. Polynucleotide immobilized support
US6300058B1 (en) 1992-01-29 2001-10-09 Hitachi Chemical Research Center, Inc. Method for measuring messenger RNA
CA2140763A1 (en) * 1992-07-28 1994-02-03 Masato Mitsuhashi Gene detection system
US5580971A (en) * 1992-07-28 1996-12-03 Hitachi Chemical Company, Ltd. Fungal detection system based on rRNA probes
US5591841A (en) * 1993-01-14 1997-01-07 Ji; Huamin Rapid purification of circular DNA by triplex-mediated affinity capture
US5610287A (en) * 1993-12-06 1997-03-11 Molecular Tool, Inc. Method for immobilizing nucleic acid molecules
US6100079A (en) * 1996-02-25 2000-08-08 Precision System Science Co., Ltd. Method for treating biopolymers, microorganisms or materials by using more than one type of magnetic particles
US5712127A (en) * 1996-04-29 1998-01-27 Genescape Inc. Subtractive amplification
WO1998051699A1 (en) * 1997-05-12 1998-11-19 Life Technologies, Inc. Methods for production and purification of nucleic acid molecules
US6399334B1 (en) 1997-09-24 2002-06-04 Invitrogen Corporation Normalized nucleic acid libraries and methods of production thereof
US6083727A (en) * 1999-06-30 2000-07-04 Incyte Pharmaceuticals Inc. Methods and compositions for producing 5' enriched cDNA libraries
CA2402534C (en) 2000-03-24 2011-05-24 Micromet Ag Mrna amplification
JP4735926B2 (ja) * 2004-05-27 2011-07-27 アイシン精機株式会社 核酸の高効率回収方法
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US11732253B2 (en) 2020-02-03 2023-08-22 Tecan Genomics, Inc. Repurposing beads in sample cleanup
GB2621159B (en) * 2022-08-04 2025-08-13 Wobble Genomics Ltd Methods of preparing processed nucleic acid samples and detecting nucleic acids and devices therefor

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1985004674A1 (en) * 1984-04-05 1985-10-24 Life Technologies, Inc. Immobilization of nucleic acids
US4734363A (en) * 1984-11-27 1988-03-29 Molecular Diagnostics, Inc. Large scale production of DNA probes
US4863849A (en) * 1985-07-18 1989-09-05 New York Medical College Automatable process for sequencing nucleotide
ZA877772B (en) * 1986-10-23 1988-04-20 Amoco Corporation Target and background capture methods and apparatus for affinity assays
IL85551A0 (en) * 1987-04-01 1988-08-31 Miles Inc Rapid hybridization assay and reagent system used therein
WO1988007585A1 (en) * 1987-04-02 1988-10-06 Genelabs, Incorporated Equal-abundance transcript composition and method

Also Published As

Publication number Publication date
AU4758590A (en) 1990-06-26
JPH04501958A (ja) 1992-04-09
WO1990006044A3 (en) 1990-11-01
ATE122721T1 (de) 1995-06-15
EP0444119A1 (de) 1991-09-04
AU627815B2 (en) 1992-09-03
CA2003500C (en) 2000-01-18
WO1990006044A2 (en) 1990-06-14
DE68922743T2 (de) 1995-10-26
CA2003500A1 (en) 1990-05-21
JP2960776B2 (ja) 1999-10-12
EP0444119B1 (de) 1995-05-17

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8315 Request for restriction filed
8318 Patent restricted
8328 Change in the person/name/address of the agent

Representative=s name: PFENNING MEINIG & PARTNER GBR, 80339 MUENCHEN

8328 Change in the person/name/address of the agent

Representative=s name: MAIWALD PATENTANWALTSGESELLSCHAFT MBH, 80335 MUENC