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DE69430919D1 - Eine phosphoenolpyruvat-carboxylasevariante, ihr gen und verfahren zur herstellung von aminosäuren - Google Patents

Eine phosphoenolpyruvat-carboxylasevariante, ihr gen und verfahren zur herstellung von aminosäuren

Info

Publication number
DE69430919D1
DE69430919D1 DE69430919T DE69430919T DE69430919D1 DE 69430919 D1 DE69430919 D1 DE 69430919D1 DE 69430919 T DE69430919 T DE 69430919T DE 69430919 T DE69430919 T DE 69430919T DE 69430919 D1 DE69430919 D1 DE 69430919D1
Authority
DE
Germany
Prior art keywords
gene
phosphoenolpyruvate carboxylase
production
amino acids
carboxylase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69430919T
Other languages
English (en)
Other versions
DE69430919T2 (de
Inventor
Masakazu Sugimoto
Tomoko Suzuki
Hiroshi Matsui
Katsura Izui
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ajinomoto Co Inc
Original Assignee
Ajinomoto Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27320552&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69430919(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Ajinomoto Co Inc filed Critical Ajinomoto Co Inc
Publication of DE69430919D1 publication Critical patent/DE69430919D1/de
Application granted granted Critical
Publication of DE69430919T2 publication Critical patent/DE69430919T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/04Alpha- or beta- amino acids
    • C12P13/08Lysine; Diaminopimelic acid; Threonine; Valine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/04Alpha- or beta- amino acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/04Alpha- or beta- amino acids
    • C12P13/06Alanine; Leucine; Isoleucine; Serine; Homoserine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/04Alpha- or beta- amino acids
    • C12P13/14Glutamic acid; Glutamine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00Preparation of nitrogen-containing organic compounds
    • C12P13/04Alpha- or beta- amino acids
    • C12P13/20Aspartic acid; Asparagine

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE69430919T 1993-08-24 1994-08-17 Eine phosphoenolpyruvat-carboxylasevariante, ihr gen und verfahren zur herstellung von aminosäuren Expired - Lifetime DE69430919T2 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP20977693 1993-08-24
JP20977593 1993-08-24
JP15387694 1994-07-05
PCT/JP1994/001365 WO1995006114A1 (fr) 1993-08-24 1994-08-17 Allele de phosphenolpyruvate carboxylase, gene de cet allele et procede de production de l'acide amine

Publications (2)

Publication Number Publication Date
DE69430919D1 true DE69430919D1 (de) 2002-08-08
DE69430919T2 DE69430919T2 (de) 2003-02-27

Family

ID=27320552

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69430919T Expired - Lifetime DE69430919T2 (de) 1993-08-24 1994-08-17 Eine phosphoenolpyruvat-carboxylasevariante, ihr gen und verfahren zur herstellung von aminosäuren

Country Status (15)

Country Link
US (2) US5876983A (de)
EP (1) EP0723011B1 (de)
KR (2) KR100337959B1 (de)
CN (1) CN1179043C (de)
AT (1) ATE220099T1 (de)
AU (1) AU682547B2 (de)
BR (1) BR9407625A (de)
CA (1) CA2169170C (de)
CZ (1) CZ289051B6 (de)
DE (1) DE69430919T2 (de)
HU (1) HU219600B (de)
MY (1) MY112250A (de)
PL (1) PL181380B1 (de)
SK (1) SK283369B6 (de)
WO (1) WO1995006114A1 (de)

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US6171833B1 (en) 1998-12-23 2001-01-09 Massachusetts Institute Of Technology Pyruvate carboxylase from corynebacterium glutamicum
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US6727411B2 (en) 1999-12-13 2004-04-27 Ajinomoto Co., Inc. Method of producing transgenic plants having improved amino acid composition
WO2001048146A1 (fr) * 1999-12-24 2001-07-05 Ajinomoto Co., Inc. Procede de production d'acide l-amine et nouveau gene
US6927046B1 (en) 1999-12-30 2005-08-09 Archer-Daniels-Midland Company Increased lysine production by gene amplification using coryneform bacteria
ES2313878T3 (es) 2000-01-21 2009-03-16 Ajinomoto Co., Inc. Procedimiento para la produccion de l-lisina.
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US7368266B2 (en) 2001-02-13 2008-05-06 Cj Corporation Method for L-threonine production
US20030115638A1 (en) * 2001-08-17 2003-06-19 Ajinomoto Co., Inc. Plants having increased amino acids content and methods for producing the same
AU2003205041A1 (en) * 2002-07-12 2004-01-29 Ajinomoto Co., Inc. Method for producing target substance by fermentation
BR0304860A (pt) * 2002-11-11 2004-08-31 Ajinomoto Kk Método para produzir uma substância alvo pela utilização de uma-bactéria pertencente ao gênero escherichia
KR100498971B1 (ko) * 2003-04-04 2005-07-04 씨제이 주식회사 염색체 내 tdcBC 및 pckA 유전자가 불활성화된 미생물 및 이를 이용하여 L-쓰레오닌을 제조하는 방법
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WO2007017710A1 (en) 2005-08-11 2007-02-15 Metabolic Explorer Process for the preparation of aspartate and derived amino acids like lysine, threonine, isoleucine, methionine, homoserine, or valine employing a microorganism with enhanced isocitrate lyase and/or malate synthase expression
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BRPI0816429A2 (pt) 2007-09-04 2019-09-24 Ajinomoto Kk microorganismo, e, método para a produção de um l-aminoácido.
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JP6459962B2 (ja) 2013-10-21 2019-01-30 味の素株式会社 L−アミノ酸の製造法
JP6519476B2 (ja) 2013-10-23 2019-05-29 味の素株式会社 目的物質の製造法
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CN105132388B (zh) * 2015-09-06 2018-02-23 江南大学 一种酶活性提高的丙酮酸羧化酶突变体r485p及其应用
CN105907730B (zh) * 2016-05-11 2019-06-21 江南大学 一种酶活性提高的丙酮酸羧化酶突变体n1078f及其应用
CN110494566A (zh) 2017-02-02 2019-11-22 嘉吉公司 产生c6-c10脂肪酸衍生物的经遗传修饰的细胞
CN111094571B (zh) * 2017-09-01 2023-10-27 国立大学法人新潟大学 龙涎香醇的有效制备方法
WO2022133917A1 (zh) * 2020-12-24 2022-06-30 武汉远大弘元股份有限公司 改造的磷酸烯醇丙酮酸羧化酶及其在提高谷氨酸棒杆菌氨基酸产量中的应用
CN115678878A (zh) * 2021-07-28 2023-02-03 梅花(上海)生物科技有限公司 一种磷酸烯醇式丙酮酸羧化酶突变体及其应用
CN115806962A (zh) * 2021-09-15 2023-03-17 中国科学院天津工业生物技术研究所 磷酸烯醇式丙酮酸羧化酶突变体及其应用
KR102614733B1 (ko) * 2023-04-06 2023-12-19 대상 주식회사 포스포에놀피루베이트 카르복실라아제 신규 변이체 및 이를 이용한 5’-이노신산 생산 방법

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JPH07108228B2 (ja) * 1990-10-15 1995-11-22 味の素株式会社 温度感受性プラスミド

Also Published As

Publication number Publication date
EP0723011A4 (de) 1996-05-21
MY112250A (en) 2001-05-31
SK20496A3 (en) 1996-11-06
SK283369B6 (sk) 2003-06-03
WO1995006114A1 (fr) 1995-03-02
US5876983A (en) 1999-03-02
DE69430919T2 (de) 2003-02-27
HUT73690A (en) 1996-09-30
HU9600240D0 (en) 1996-03-28
CN1133615A (zh) 1996-10-16
EP0723011A1 (de) 1996-07-24
CN1179043C (zh) 2004-12-08
KR100337959B1 (ko) 2002-11-23
AU8099194A (en) 1995-03-21
CA2169170A1 (en) 1995-03-02
KR960704029A (ko) 1996-08-31
EP0723011B1 (de) 2002-07-03
CZ52496A3 (en) 1996-06-12
US5919694A (en) 1999-07-06
PL181380B1 (pl) 2001-07-31
AU682547B2 (en) 1997-10-09
HU219600B (hu) 2001-05-28
CA2169170C (en) 2007-01-09
ATE220099T1 (de) 2002-07-15
BR9407625A (pt) 1997-01-21
PL313119A1 (en) 1996-06-10
CZ289051B6 (cs) 2001-10-17

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