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DE69433210D1 - VERFAHEN ZUR GLEICHZEITIGEN IDENTIFIKATION VON DIFFERENTIELLE EXPRIMIERTEN mRNA'S UND ZUR MESSUNG IHRER RELATIVEN KONZENTRATION - Google Patents

VERFAHEN ZUR GLEICHZEITIGEN IDENTIFIKATION VON DIFFERENTIELLE EXPRIMIERTEN mRNA'S UND ZUR MESSUNG IHRER RELATIVEN KONZENTRATION

Info

Publication number
DE69433210D1
DE69433210D1 DE69433210T DE69433210T DE69433210D1 DE 69433210 D1 DE69433210 D1 DE 69433210D1 DE 69433210 T DE69433210 T DE 69433210T DE 69433210 T DE69433210 T DE 69433210T DE 69433210 D1 DE69433210 D1 DE 69433210D1
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DE
Germany
Prior art keywords
cdna
mrna
primers
crna
derived
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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DE69433210T
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English (en)
Inventor
Mark G Erlander
Gregor J Sutcliffe
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Scripps Research Institute
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Scripps Research Institute
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Publication of DE69433210D1 publication Critical patent/DE69433210D1/de
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69433210T 1993-11-12 1994-11-14 VERFAHEN ZUR GLEICHZEITIGEN IDENTIFIKATION VON DIFFERENTIELLE EXPRIMIERTEN mRNA'S UND ZUR MESSUNG IHRER RELATIVEN KONZENTRATION Expired - Lifetime DE69433210D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/152,482 US5459037A (en) 1993-11-12 1993-11-12 Method for simultaneous identification of differentially expressed mRNAs and measurement of relative concentrations
PCT/US1994/013041 WO1995013369A1 (en) 1993-11-12 1994-11-14 METHOD FOR SIMULTANEOUS IDENTIFICATION OF DIFFERENTIALLY EXPRESSED mRNAs AND MEASUREMENT OF RELATIVE CONCENTRATIONS

Publications (1)

Publication Number Publication Date
DE69433210D1 true DE69433210D1 (de) 2003-11-06

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
DE69433210T Expired - Lifetime DE69433210D1 (de) 1993-11-12 1994-11-14 VERFAHEN ZUR GLEICHZEITIGEN IDENTIFIKATION VON DIFFERENTIELLE EXPRIMIERTEN mRNA'S UND ZUR MESSUNG IHRER RELATIVEN KONZENTRATION

Country Status (9)

Country Link
US (6) US5459037A (de)
EP (1) EP0726946B1 (de)
JP (1) JPH09509306A (de)
AT (1) ATE251220T1 (de)
CA (1) CA2174966A1 (de)
DE (1) DE69433210D1 (de)
FI (1) FI962000A0 (de)
NO (1) NO961902L (de)
WO (1) WO1995013369A1 (de)

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Also Published As

Publication number Publication date
WO1995013369A1 (en) 1995-05-18
US5459037A (en) 1995-10-17
EP0726946A4 (de) 1999-02-10
NO961902D0 (no) 1996-05-10
EP0726946B1 (de) 2003-10-01
EP0726946A1 (de) 1996-08-21
US20030092006A1 (en) 2003-05-15
AU1055195A (en) 1995-05-29
FI962000A7 (fi) 1996-05-10
AU687127B2 (en) 1998-02-19
ATE251220T1 (de) 2003-10-15
JPH09509306A (ja) 1997-09-22
FI962000L (fi) 1996-05-10
FI962000A0 (fi) 1996-05-10
US5807680A (en) 1998-09-15
US6309834B1 (en) 2001-10-30
US20020127571A1 (en) 2002-09-12
NO961902L (no) 1996-07-12
US6030784A (en) 2000-02-29
CA2174966A1 (en) 1995-05-18

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