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DE69533895D1 - Verfahren zur genauen zählung von proben-hybridisaten im zellkern - Google Patents

Verfahren zur genauen zählung von proben-hybridisaten im zellkern

Info

Publication number
DE69533895D1
DE69533895D1 DE69533895T DE69533895T DE69533895D1 DE 69533895 D1 DE69533895 D1 DE 69533895D1 DE 69533895 T DE69533895 T DE 69533895T DE 69533895 T DE69533895 T DE 69533895T DE 69533895 D1 DE69533895 D1 DE 69533895D1
Authority
DE
Germany
Prior art keywords
fluorescent
cells
probe spots
values
gating
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69533895T
Other languages
English (en)
Inventor
Louis A Kamentsky
Lee D Kamentsky
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Compucyte Corp
Original Assignee
Compucyte Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Compucyte Corp filed Critical Compucyte Corp
Application granted granted Critical
Publication of DE69533895D1 publication Critical patent/DE69533895D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE69533895T 1994-10-17 1995-10-13 Verfahren zur genauen zählung von proben-hybridisaten im zellkern Expired - Lifetime DE69533895D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/324,265 US5523207A (en) 1994-10-17 1994-10-17 Method for accurate counting of probe spots in cell nuclei
PCT/US1995/013132 WO1996012043A1 (en) 1994-10-17 1995-10-13 Method for accurate counting of probe spots in cell nuclei

Publications (1)

Publication Number Publication Date
DE69533895D1 true DE69533895D1 (de) 2005-02-03

Family

ID=23262826

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69533895T Expired - Lifetime DE69533895D1 (de) 1994-10-17 1995-10-13 Verfahren zur genauen zählung von proben-hybridisaten im zellkern

Country Status (8)

Country Link
US (1) US5523207A (de)
EP (1) EP0787204B1 (de)
AT (1) ATE286142T1 (de)
AU (1) AU3831595A (de)
CA (1) CA2208256A1 (de)
DE (1) DE69533895D1 (de)
IL (1) IL115507A (de)
WO (1) WO1996012043A1 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5266495A (en) 1990-03-02 1993-11-30 Cytyc Corporation Method and apparatus for controlled instrumentation of particles with a filter device
DE69417899T2 (de) * 1994-11-17 1999-11-04 Chemunex, Maisons-Alfort Vorrichtung und Verfahren zum Erkennen und Zählen von selten vorkommenden Säugerzellen
US5863504A (en) * 1995-03-16 1999-01-26 Bio-Rad Laboratories, Inc. Fluorescence imaging instrument utilizing fish
US6207024B1 (en) 1999-10-04 2001-03-27 Astaris Llc Method of preparing phosphorus
US7369304B2 (en) * 1999-10-29 2008-05-06 Cytyc Corporation Cytological autofocusing imaging systems and methods
US6348325B1 (en) 1999-10-29 2002-02-19 Cytyc Corporation Cytological stain composition
US6661501B1 (en) 1999-10-29 2003-12-09 Cytyc Corporation Cytological stain composition including verification characteristic
US6665060B1 (en) * 1999-10-29 2003-12-16 Cytyc Corporation Cytological imaging system and method
US6593102B2 (en) 1999-10-29 2003-07-15 Cytyc Corporation Cytological stain composition
CA2454866A1 (en) * 2001-06-12 2002-12-19 Board Of Regents, The University Of Texas System Methods of detecting, diagnosing and treating cancer and identifying neoplastic progression
US6583424B2 (en) * 2001-06-25 2003-06-24 Agilent Technologies Inc. Scanning system with calibrated detection and method
FR2831275B1 (fr) * 2001-10-18 2004-01-30 Commissariat Energie Atomique Substrat revetu d'un film organique transparent et procede de fabrication
US7300798B2 (en) * 2001-10-18 2007-11-27 Agilent Technologies, Inc. Chemical arrays
US20030092008A1 (en) * 2001-11-14 2003-05-15 Bell Michael L. Method and apparatus for multiplex flow cytometry analysis of diverse mixed analytes from bodily fluid samples
US20030143524A1 (en) * 2002-01-31 2003-07-31 Boaz Lerner Method and system for determining the amount, distribution and conformation of genetic material in a cell
EP1488229B1 (de) * 2002-03-13 2012-10-24 Q3Dm, Llc System und verfahren zur automatischen farbsegmentierung und minimalen signifikanten antwort für die messung fraktionierter lokalisierter intensität zellulärer kompartimente
US7706590B2 (en) * 2004-01-28 2010-04-27 Compucyte Corporation Method and device for interrogating samples using laser scanning cytometry and other techniques
US7468796B2 (en) * 2004-08-06 2008-12-23 Compucyte Corporation Multiple-color monochromatic light absorption and quantification of light absorption in a stained sample
JP4418362B2 (ja) * 2004-12-28 2010-02-17 オリンパス株式会社 画像処理装置
US7804594B2 (en) * 2006-12-29 2010-09-28 Abbott Laboratories, Inc. Method and apparatus for rapidly counting and identifying biological particles in a flow stream
ATE528722T1 (de) 2007-05-14 2011-10-15 Historx Inc Abteilungsauftrennung durch pixelcharakterisierung unter verwendung von bilddatenclusterung
CA2690633C (en) * 2007-06-15 2015-08-04 Historx, Inc. Method and system for standardizing microscope instruments
CA2604317C (en) 2007-08-06 2017-02-28 Historx, Inc. Methods and system for validating sample images for quantitative immunoassays
CA2596204C (en) * 2007-08-07 2019-02-26 Historx, Inc. Method and system for determining an optimal dilution of a reagent
US7978258B2 (en) * 2007-08-31 2011-07-12 Historx, Inc. Automatic exposure time selection for imaging tissue
US8159670B2 (en) 2007-11-05 2012-04-17 Abbott Laboratories Method and apparatus for rapidly counting and identifying biological particles in a flow stream
WO2010033508A1 (en) * 2008-09-16 2010-03-25 Historx, Inc. Reproducible quantification of biomarker expression
US20110039735A1 (en) * 2009-08-13 2011-02-17 Agilent Technologies, Inc. Probe design for oligonucleotide fluorescence in situ hybridization (fish)
US9042631B2 (en) * 2013-01-24 2015-05-26 General Electric Company Method and systems for cell-level fish dot counting
CN111175267A (zh) * 2020-01-18 2020-05-19 珠海圣美生物诊断技术有限公司 基于fish技术的细胞判读方法和系统

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4021117A (en) * 1975-08-07 1977-05-03 Hildegard Gohde Process for automatic counting and measurement of particles
US5072382A (en) * 1989-10-02 1991-12-10 Kamentsky Louis A Methods and apparatus for measuring multiple optical properties of biological specimens
JPH06502079A (ja) * 1991-09-19 1994-03-10 ヴァイシス・インコーポレーテッド ゲノム識別用のプローブ組成物および方法
US5326691A (en) * 1991-11-21 1994-07-05 John Hozier Micro-libraries and methods of making and manipulating them methods for generating and analyzing micro-libraries
US5427910A (en) * 1992-12-09 1995-06-27 Compucyte Corporation Method of cytogenetic analysis

Also Published As

Publication number Publication date
IL115507A (en) 2000-10-31
AU3831595A (en) 1996-05-06
ATE286142T1 (de) 2005-01-15
US5523207A (en) 1996-06-04
EP0787204B1 (de) 2004-12-29
EP0787204A4 (de) 2002-01-16
EP0787204A1 (de) 1997-08-06
WO1996012043A1 (en) 1996-04-25
IL115507A0 (en) 1996-01-19
CA2208256A1 (en) 1996-04-25

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