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DE69629077D1 - Thermostabile DNA Polymerase - Google Patents

Thermostabile DNA Polymerase

Info

Publication number
DE69629077D1
DE69629077D1 DE69629077T DE69629077T DE69629077D1 DE 69629077 D1 DE69629077 D1 DE 69629077D1 DE 69629077 T DE69629077 T DE 69629077T DE 69629077 T DE69629077 T DE 69629077T DE 69629077 D1 DE69629077 D1 DE 69629077D1
Authority
DE
Germany
Prior art keywords
dna polymerase
polymerase
thermostable dna
thermus
homology
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69629077T
Other languages
English (en)
Other versions
DE69629077T2 (de
Inventor
Maria Davis
Carl Fuller
Bruce Moffett
John James Akron Cunniff
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Global Life Sciences Solutions USA LLC
Original Assignee
Amersham Biosciences Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Biosciences Corp filed Critical Amersham Biosciences Corp
Publication of DE69629077D1 publication Critical patent/DE69629077D1/de
Application granted granted Critical
Publication of DE69629077T2 publication Critical patent/DE69629077T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Pulleys (AREA)
DE69629077T 1995-05-31 1996-05-30 Thermostabile DNA Polymerase Expired - Lifetime DE69629077T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US455686 1989-12-22
US45568695A 1995-05-31 1995-05-31

Publications (2)

Publication Number Publication Date
DE69629077D1 true DE69629077D1 (de) 2003-08-21
DE69629077T2 DE69629077T2 (de) 2004-04-22

Family

ID=23809855

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69629077T Expired - Lifetime DE69629077T2 (de) 1995-05-31 1996-05-30 Thermostabile DNA Polymerase

Country Status (8)

Country Link
US (1) US5885813A (de)
EP (1) EP0745676B1 (de)
JP (1) JP3966555B2 (de)
AT (1) ATE245193T1 (de)
CA (1) CA2222744C (de)
DE (1) DE69629077T2 (de)
GB (1) GB2301590B (de)
WO (1) WO1996038568A1 (de)

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US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
US5912155A (en) * 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana
AU7236296A (en) * 1995-09-08 1997-03-27 Life Technologies, Inc. Cloned dna polymerases from thermotoga and mutants thereof
US6818431B1 (en) 1995-10-18 2004-11-16 Shanghai Mendel Dna Center Co. Ltd. DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
US6165765A (en) * 1995-10-18 2000-12-26 Shanghai Institute Of Biochemistry, Chinese Academy Of Sciences DNA polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides
EP1007730A4 (de) * 1996-08-30 2004-04-28 Invitrogen Corp Verfahren zur identifizierung und isolierung von spezifischen nukleotidsequenzen in cdna und genomischer dna
US6306588B1 (en) * 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
US6265193B1 (en) * 1997-03-12 2001-07-24 Pe Corporation (Ny) DNA polymerases having improved labeled nucleotide incorporation properties
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6130045A (en) * 1998-06-11 2000-10-10 Clontech Laboratories, Inc. Thermostable polymerase
JP4349545B2 (ja) * 1998-06-17 2009-10-21 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション Ｆｙ７ポリメラーゼ
WO2000068411A1 (en) * 1999-05-12 2000-11-16 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
US7501245B2 (en) * 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
GB9918150D0 (en) * 1999-08-03 1999-10-06 Univ Sheffield Nuclease variant
AU6768700A (en) * 1999-08-21 2001-03-19 Amersham Pharmacia Biotech Inc. Taq dna polymerases having an amino acid substitution at e681 and homologs thereof exhibiting improved salt tolerance
ATE321867T1 (de) * 1999-10-29 2006-04-15 Stratagene California Zusammensetzungen und methoden zur verwendung von dna polymerasen
US20040009486A1 (en) 1999-10-29 2004-01-15 Sorge Joseph A. Compositions and methods utilizing DNA polymerases
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
AU2001296645A1 (en) 2000-10-06 2002-04-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
WO2002072892A1 (en) 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
WO2003025132A2 (en) * 2001-09-14 2003-03-27 Invitrogen Corporation Dna polymerases and mutants thereof
US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
AU2002352902A1 (en) 2001-11-30 2003-06-17 Applera Corporation Thermus thermophilus nucleic acid polymerases
AU2002346498A1 (en) 2001-11-30 2003-06-17 Applera Corporation Thermus brockianus nucleic acid polymerases
EP1474532A4 (de) * 2002-02-12 2005-05-04 Applera Corp Polymerasezusammensetzungen
EP1616033B1 (de) 2003-03-25 2010-03-03 Stratagene California Dna-polymerasefusionen und deren verwendungen
US20050170367A1 (en) * 2003-06-10 2005-08-04 Quake Stephen R. Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
AU2004203649B2 (en) 2003-08-12 2006-01-12 F. Hoffmann-La Roche Ag Thermostable Taq polymerase fragment
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US20060172408A1 (en) * 2003-12-01 2006-08-03 Quake Steven R Device for immobilizing chemical and biochemical species and methods of using same
EP2287341B1 (de) 2003-12-01 2013-02-13 Life Technologies Corporation Rekombinationsstellen enthaltende Nukleinsäuremoleküle und Verfahren zur Verwendung davon
EP1716254B1 (de) 2004-02-19 2010-04-07 Helicos Biosciences Corporation Verfahren zur analyse von polynukleotidsequenzen
US20050196392A1 (en) * 2004-02-20 2005-09-08 Andersen Mark R. Lesion repair polymerase compositions
US7417133B2 (en) * 2004-02-27 2008-08-26 Institut Pasteur Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications of the same
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US20050239085A1 (en) * 2004-04-23 2005-10-27 Buzby Philip R Methods for nucleic acid sequence determination
US20050260609A1 (en) * 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
US7476734B2 (en) * 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
ATE507305T1 (de) * 2004-05-25 2011-05-15 Helicos Biosciences Corp Verfahren zur nukleinsäureimmobilisierung
US20070117104A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070117103A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20060024678A1 (en) * 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
US20060118754A1 (en) * 2004-12-08 2006-06-08 Lapen Daniel C Stabilizing a polyelectrolyte multilayer
US20080305142A1 (en) * 2004-12-11 2008-12-11 Cytogenix, Inc. Cell Free Biosynthesis of High-Quality Nucleic Acid and Uses Thereof
US7220549B2 (en) * 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US20060172328A1 (en) * 2005-01-05 2006-08-03 Buzby Philip R Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction
AU2006204006A1 (en) 2005-01-06 2006-07-13 Applera Corporation Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US20060263790A1 (en) * 2005-05-20 2006-11-23 Timothy Harris Methods for improving fidelity in a nucleic acid synthesis reaction
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US20070059713A1 (en) 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
US20070117102A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070128610A1 (en) * 2005-12-02 2007-06-07 Buzby Philip R Sample preparation method and apparatus for nucleic acid sequencing
WO2011027966A2 (en) 2009-09-03 2011-03-10 Seegene, Inc. Td probe and its uses
EP2582804B1 (de) 2010-06-18 2015-02-25 Roche Diagnostics GmbH Dna polymerasen mit erhöhter unterscheidung von 3' fehlpaarungen
US20240102067A1 (en) * 2022-09-26 2024-03-28 Illumina, Inc. Resynthesis Kits and Methods

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5466591A (en) * 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US4889818A (en) * 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5079352A (en) * 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US4795699A (en) * 1987-01-14 1989-01-03 President And Fellows Of Harvard College T7 DNA polymerase
US5075216A (en) * 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
JP2997043B2 (ja) * 1989-04-12 2000-01-11 プレジデント アンド フェロウズ オブ ハーバード カレッジ 改良されたプライマー伸長反応
DE69030386T2 (de) * 1989-12-22 1997-10-09 F. Hoffmann-La Roche Ag, Basel Bei hoher temperatur aktive reverse transkriptasen
DK0550687T3 (da) * 1990-09-28 1999-12-13 Hoffmann La Roche 5' til 3'-exonukleasemutationer af termostabile DNA-polymeraser
DE553264T1 (de) * 1990-10-05 1994-04-28 Wayne M Barnes Thermostabile dna polymerase.
FI923911A7 (fi) * 1992-09-01 1994-03-02 Timo Korpela DNA-molekyylien in vitro-synteesi
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5474920A (en) * 1993-11-23 1995-12-12 State Of Oregon, Acting By And Through The Oregon State Board Of Higher Education On Behalf Of The Oregon Health Sciences University Modified thermo-resistant DNA polymerases
DE69400567T2 (de) * 1994-10-17 1997-02-06 President And Fellows Of Harvard College, Cambridge, Mass. DNS Polymerase mit veränderter Nukleotid-Bindungstelle

Also Published As

Publication number Publication date
ATE245193T1 (de) 2003-08-15
CA2222744C (en) 2008-03-25
DE69629077T2 (de) 2004-04-22
JPH11507211A (ja) 1999-06-29
US5885813A (en) 1999-03-23
EP0745676A1 (de) 1996-12-04
CA2222744A1 (en) 1996-12-05
GB2301590B (en) 1997-07-09
GB9611218D0 (en) 1996-07-31
EP0745676B1 (de) 2003-07-16
JP3966555B2 (ja) 2007-08-29
WO1996038568A1 (en) 1996-12-05
GB2301590A (en) 1996-12-11

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: GE HEALTHCARE BIO-SCIENCES CORP., PISCATAWAY, N.J.