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EA201490216A1 - Варианты альфа-амилазы и кодирующие их полинуклеотиды - Google Patents

Варианты альфа-амилазы и кодирующие их полинуклеотиды

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Publication number
EA201490216A1
EA201490216A1 EA201490216A EA201490216A EA201490216A1 EA 201490216 A1 EA201490216 A1 EA 201490216A1 EA 201490216 A EA201490216 A EA 201490216A EA 201490216 A EA201490216 A EA 201490216A EA 201490216 A1 EA201490216 A1 EA 201490216A1
Authority
EA
Eurasian Patent Office
Prior art keywords
variants
alpha
amilase
polynucleotide code
polynucleotide
Prior art date
Application number
EA201490216A
Other languages
English (en)
Inventor
Томоко Мацуи
Аки Томики
Джуллермо Кауард-Келли
Original Assignee
Новозимс А/С
Новозаймз Норт Америка, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Новозимс А/С, Новозаймз Норт Америка, Инк. filed Critical Новозимс А/С
Publication of EA201490216A1 publication Critical patent/EA201490216A1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01001—Alpha-amylase (3.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)

Abstract

Изобретение относится к вариантам альфа-амилазы. Настоящее изобретение также относится к полинуклеотидам, кодирующим указанные варианты; к нуклеотидным конструкциям, векторам и клеткам-хозяевам, содержащим указанные полинуклеотиды, а также к способам применения таких вариантов.
EA201490216A 2011-07-06 2012-07-06 Варианты альфа-амилазы и кодирующие их полинуклеотиды EA201490216A1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161504771P 2011-07-06 2011-07-06
US201161505192P 2011-07-07 2011-07-07
PCT/US2012/045670 WO2013006756A2 (en) 2011-07-06 2012-07-06 Alpha amylase variants and polynucleotides encoding same

Publications (1)

Publication Number Publication Date
EA201490216A1 true EA201490216A1 (ru) 2014-07-30

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
EA201490216A EA201490216A1 (ru) 2011-07-06 2012-07-06 Варианты альфа-амилазы и кодирующие их полинуклеотиды

Country Status (10)

Country Link
US (2) US9267124B2 (ru)
EP (1) EP2729572B1 (ru)
CN (1) CN103781910B (ru)
BR (1) BR112014000143B1 (ru)
CA (1) CA2840962C (ru)
DK (1) DK2729572T3 (ru)
EA (1) EA201490216A1 (ru)
ES (1) ES2729385T3 (ru)
MX (1) MX337984B (ru)
WO (1) WO2013006756A2 (ru)

Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2822637C (en) 2010-12-22 2020-06-30 Novozymes North America, Inc. Processes for producing fermentation products
EA201490216A1 (ru) * 2011-07-06 2014-07-30 Новозимс А/С Варианты альфа-амилазы и кодирующие их полинуклеотиды
US9856498B2 (en) 2012-03-30 2018-01-02 Novozymes A/S Processes of producing fermentation products
ES2935920T3 (es) 2012-03-30 2023-03-13 Novozymes North America Inc Procesos de elaboración de productos de fermentación
CN105208869A (zh) 2013-04-05 2015-12-30 诺维信公司 用α-淀粉酶、脂肪酶和磷脂酶生产烘焙产品的方法
CA2915065C (en) * 2013-06-24 2022-11-01 Novozymes A/S Processes for recovering oil from fermentation product processes and processes for producing fermentation products
US11939552B2 (en) 2013-06-24 2024-03-26 Novozymes A/S Process of recovering oil
WO2015007639A1 (en) 2013-07-17 2015-01-22 Novozymes A/S Pullulanase chimeras and polynucleotides encoding same
EP3039131A4 (en) * 2013-08-30 2017-02-08 Novozymes A/S Enzyme composition and uses thereof
EP3097192B1 (en) 2014-01-22 2018-07-25 Novozymes A/S Pullulanase variants and polynucleotides encoding same
EP3209788B1 (en) 2014-10-23 2019-06-05 Novozymes A/S Glucoamylase variants and polynucleotides encoding same
US10308963B2 (en) 2015-02-27 2019-06-04 Novozymes A/S Processes of producing ethanol using a fermenting organism
WO2016196202A1 (en) 2015-05-29 2016-12-08 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
EP3310910A1 (en) 2015-06-18 2018-04-25 Novozymes A/S Polypeptides having trehalase activity and the use thereof in process of producing fermentation products
US20180208916A1 (en) 2015-07-23 2018-07-26 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
US10689630B2 (en) 2015-12-22 2020-06-23 Novozymes A/S Processes for producing fermentation products
JP2019506186A (ja) 2016-02-26 2019-03-07 南京百斯杰生物工程有限公司Ｎａｎｊｉｎｇ Ｂｅｓｔｚｙｍｅ Ｂｉｏ−Ｅｎｇｉｎｅｅｒｉｎｇ Ｃｏ．，Ｌｔｄ． α−アミラーゼバリアントおよびその使用
DK3487996T5 (da) 2016-07-21 2024-09-02 Novozymes As Serinproteasevarianter og polynukleotider, der koder for samme
CA3029479A1 (en) 2016-07-21 2018-01-25 Novozymes A/S Serine protease variants and polynucleotides encoding same
EP3526336A1 (en) 2016-10-17 2019-08-21 Novozymes A/S Methods of reducing foam during ethanol fermentation
CN110072997A (zh) 2016-11-23 2019-07-30 诺维信公司 具有蛋白酶活性的多肽和编码其的多核苷酸
WO2018098381A1 (en) 2016-11-23 2018-05-31 Novozymes A/S Improved yeast for ethanol production
CN110177873A (zh) 2016-11-29 2019-08-27 诺维信公司 α-淀粉酶变体以及对其进行编码的多核苷酸
BR112019021223A2 (pt) 2017-04-11 2020-04-28 Novozymes As variante de glucoamilase, métodos para aumentar a atividade de hidrólise de amido em bruto de uma glucoamilase e para produzir uma variante de glucoamilase, composição, uso de uma variante de glucoamilase, processos para produzir um produto de fermentação e para produzir um produto de xarope, polinucleotídeo codificando a variante de glucoamilase, construto de ácido nucleico, vetor de expressão, e, célula hospedeira.
US20200157581A1 (en) 2017-06-02 2020-05-21 Novozymes A/S Improved Yeast For Ethanol Production
EP4015524A1 (en) 2017-06-28 2022-06-22 Novozymes A/S Polypeptides having trehalase activity and polynucleotides encoding same
MX2020001282A (es) 2017-08-08 2020-03-12 Novozymes As Polipeptidos que tienen actividad trehalasa y uso de los mismos en proceso de produccion de productos de fermentacion.
US20200181663A1 (en) 2017-08-30 2020-06-11 Novozymes A/S Combined use of at least one endoprotease and at least one exo-protease in an ssf process for improving ethanol yield
EP3692150A1 (en) 2017-10-04 2020-08-12 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
CA3081096A1 (en) 2017-12-08 2019-06-13 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
CN111601887A (zh) 2017-12-08 2020-08-28 诺维信公司 α-淀粉酶变体以及对其进行编码的多核苷酸
CN113286871A (zh) 2018-01-29 2021-08-20 诺维信公司 用于乙醇生产的氮利用提高的微生物
CN112272707B (zh) 2018-02-15 2024-07-26 诺维信公司 用于乙醇生产的改善的酵母
CA3096900A1 (en) * 2018-04-09 2019-10-17 Novozymes A/S Polypeptides having alpha-amylase activity and polynucleotides encoding same
WO2019231944A2 (en) 2018-05-31 2019-12-05 Novozymes A/S Processes for enhancing yeast growth and productivity
CN112368393A (zh) 2018-07-11 2021-02-12 诺维信公司 用于生产发酵产物的方法
BR112021001282A2 (pt) 2018-07-25 2021-04-27 Novozymes A/S levedura expressando enzimas para produção de etanol
WO2020076697A1 (en) 2018-10-08 2020-04-16 Novozymes A/S Enzyme-expressing yeast for ethanol production
CA3128139A1 (en) 2019-03-18 2020-09-24 Novozymes A/S Polypeptides having pullulanase activity suitable for use in liquefaction
CN113853438A (zh) 2019-04-02 2021-12-28 诺维信公司 用于生产发酵产物的方法
AU2020321930A1 (en) 2019-07-26 2022-01-06 Novozymes A/S Microorganisms with improved nitrogen transport for ethanol production
BR112022002203A2 (pt) 2019-08-05 2022-09-06 Novozymes As Misturas de enzimas e processos para produção de um ingrediente alimentar com alto teor de proteína a partir de um subproduto de vinhaça completa
US12275967B2 (en) 2019-09-16 2025-04-15 Novozymes A/S Processes for producing fermentation products and compositions used therein
MX2022006963A (es) 2019-12-10 2022-07-12 Novozymes As Microorganismo para fermentacion de pentosa mejorada.
CN113122592B (zh) * 2019-12-30 2025-05-20 中国科学院天津工业生物技术研究所 一种淀粉或其衍生物的酶法催化利用方法
US20230142991A1 (en) 2020-02-10 2023-05-11 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
US20240228996A1 (en) 2020-02-10 2024-07-11 Novozymes A/S Polypeptides having alpha-amylase activity and polynucleotides encoding same
WO2021163036A1 (en) 2020-02-10 2021-08-19 Novozymes A/S Raw starch hydrolysis process for producing a fermentation product
AR123983A1 (es) 2020-11-02 2023-02-01 Novozymes As Variantes de glucoamilasa y polinucleótidos que codifican las mismas
CN113307885B (zh) * 2021-05-26 2022-09-27 江南大学 一种产物特异性提高的融合蛋白及其制备直链麦芽五糖的应用
BR112023025624A2 (pt) 2021-06-07 2024-02-27 Novozymes As Célula de levedura recombinante, célula hospedeira recombinante, composição, cocultura, métodos de produção de um derivado de uma célula hospedeira recombinante e de produção de um produto de fermentação, e, uso de uma célula hospedeira recombinante
WO2024088549A1 (en) 2022-10-24 2024-05-02 Novozymes A/S Baking method with thermostable amg variant and alpha-amylase
AU2022483870A1 (en) 2022-10-24 2025-04-10 Novozymes A/S Baking method for pulse protein fortified bread employing thermostable amyloglucosidase variante (ec 3.2.1.3)
CN120380139A (zh) 2022-12-19 2025-07-25 诺维信公司 具有乙酰木聚糖酯酶活性的碳水化合物酯酶家族3（ce3）多肽和编码其的多核苷酸
CN120283060A (zh) 2022-12-19 2025-07-08 诺维信公司 用于使用纤维降解酶和工程化酵母生产发酵产物的工艺
CN120380158A (zh) 2022-12-19 2025-07-25 诺维信公司 用于降低发酵产物生产方法后端的浆体粘度的方法
CN120380140A (zh) 2022-12-19 2025-07-25 诺维信公司 具有阿魏酸酯酶和/或乙酰木聚糖酯酶活性的碳水化合物酯酶家族1（ce1）多肽和编码其的多核苷酸
WO2024137248A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Compositions comprising arabinofuranosidases and a xylanase, and use thereof for increasing hemicellulosic fiber solubilization
WO2024258820A2 (en) 2023-06-13 2024-12-19 Novozymes A/S Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase
WO2025128568A1 (en) 2023-12-11 2025-06-19 Novozymes A/S Composition and use thereof for increasing hemicellulosic fiber solubilization

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5534046A (en) 1978-09-01 1980-03-10 Cpc International Inc Novel glucoamyrase having excellent heat resistance and production
NO840200L (no) 1983-01-28 1984-07-30 Cefus Corp Glukoamylase cdna.
DK135983D0 (da) 1983-03-25 1983-03-25 Novo Industri As Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse
US4587215A (en) 1984-06-25 1986-05-06 Uop Inc. Highly thermostable amyloglucosidase
JPS62126989A (ja) 1985-11-26 1987-06-09 Godo Shiyusei Kk コルテイシウム属担子菌の生産する酵素を用いた澱粉質の無蒸煮糖化法
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
JP2703598B2 (ja) 1987-09-04 1998-01-26 ノボ―ノルディスク アクティーゼルスカブ アスペルギルス菌中でのタンパク質生産物の生産方法およびアスペルギルス菌中で使用するためのプロモーター
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
DE3909096A1 (de) 1989-03-20 1990-09-27 Garabed Antranikian Alpha-amylase
IL97645A (en) 1990-03-23 1997-03-18 Gist Brocades Nv Production of enzymes in seeds and their use
US5162210A (en) 1990-06-29 1992-11-10 Iowa State University Research Foundation Process for enzymatic hydrolysis of starch to glucose
US6395966B1 (en) 1990-08-09 2002-05-28 Dekalb Genetics Corp. Fertile transgenic maize plants containing a gene encoding the pat protein
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
ATE444356T1 (de) * 1992-07-23 2009-10-15 Novozymes As Mutierte -g(a)-amylase, waschmittel und geschirrspülmittel
BR9407767A (pt) * 1993-10-08 1997-03-18 Novo Nordisk As Variante de enzima &-amilase uso da mesma construção de DNA vetor de express o recombinante célula processos para produzir uma &-amilase hibrida e para preparar uma variante de uma &-amilase aditivo detergente e composições detergentes
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
DK0753057T3 (da) 1994-03-29 2006-01-30 Novozymes As Alkalisk Bacillus-amylase
ATE206460T1 (de) 1994-06-03 2001-10-15 Novo Nordisk Biotech Inc Gereinigte myceliophthora laccasen und nukleinsäuren dafür kodierend
DE69534185T2 (de) 1994-06-30 2006-02-23 Novozymes Biotech, Inc., Davis Nicht-toxisches, nicht-toxigenes, nicht-pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
AR000862A1 (es) * 1995-02-03 1997-08-06 Novozymes As Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
ATE432342T1 (de) 1995-02-03 2009-06-15 Novozymes As Eine methode zum entwurf von alpha-amylase mutanten mit vorbestimmten eigenschaften
US6093562A (en) 1996-02-05 2000-07-25 Novo Nordisk A/S Amylase variants
JP4290763B2 (ja) 1996-04-30 2009-07-08 ノボザイムス アクティーゼルスカブ α―アミラーゼ変異体
KR20010015754A (ko) 1997-10-13 2001-02-26 한센 핀 베네드, 안네 제헤르, 웨이콥 마리안느 α-아밀라제 변이체
AU1434299A (en) 1997-11-26 1999-06-16 Novo Nordisk A/S Thermostable glucoamylase
CN103352033B (zh) 1998-02-27 2016-05-11 诺维信公司 麦芽α淀粉酶变体
MXPA01000352A (es) 1998-07-15 2002-06-04 Novozymes As Varientes de glucoamilasa.
ATE332968T1 (de) 1998-10-26 2006-08-15 Novozymes As Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen
CN100510096C (zh) 1999-03-22 2009-07-08 诺沃奇梅兹有限公司 用于在真菌细胞中表达基因的启动子
ES2286012T3 (es) 1999-03-30 2007-12-01 Novozymes A/S Variantes de alfa-amilasa.
EP2009098A1 (en) 1999-07-09 2008-12-31 Novozymes A/S Glucoamylase variant
EP2204446A1 (en) 2000-08-01 2010-07-07 Novozymes A/S Alpha-amylase mutants with altered properties
US7151204B2 (en) 2001-01-09 2006-12-19 Monsanto Technology Llc Maize chloroplast aldolase promoter compositions and methods for use thereof
US7314712B2 (en) 2001-07-27 2008-01-01 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Systems for in vivo site-directed mutagenesis using oligonucleotides
JP4550587B2 (ja) * 2002-12-17 2010-09-22 ノボザイムス アクティーゼルスカブ 熱安定性α−アミラーゼ
ES2245621T3 (es) 2003-03-10 2010-05-19 Novozymes A/S Procesos para un producto alcoholico.
WO2005003311A2 (en) 2003-06-25 2005-01-13 Novozymes A/S Enzymes for starch processing
ATE549403T1 (de) 2003-06-25 2012-03-15 Novozymes As Enzyme zur stärkeverarbeitung
JP5452869B2 (ja) 2004-12-22 2014-03-26 ノボザイムス アクティーゼルスカブ デンプン加工法
WO2009030728A2 (en) * 2007-09-05 2009-03-12 Novozymes A/S Enzyme compositions with stabilizing constituent
EA201490216A1 (ru) * 2011-07-06 2014-07-30 Новозимс А/С Варианты альфа-амилазы и кодирующие их полинуклеотиды

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CA2840962A1 (en) 2013-01-10
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