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EP1831670A4 - Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences - Google Patents

Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences

Info

Publication number
EP1831670A4
EP1831670A4 EP05855662A EP05855662A EP1831670A4 EP 1831670 A4 EP1831670 A4 EP 1831670A4 EP 05855662 A EP05855662 A EP 05855662A EP 05855662 A EP05855662 A EP 05855662A EP 1831670 A4 EP1831670 A4 EP 1831670A4
Authority
EP
European Patent Office
Prior art keywords
electrodes
dna sequence
recognition
target dna
polynucleotide sequences
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP05855662A
Other languages
German (de)
French (fr)
Other versions
EP1831670A2 (en
Inventor
Jean-Christophe P Gabriel
Shripal Gandhi
George Gruner
Charles Steven Joiner Jr
Ron Sosnowski
Alexander Star
Eugene Tu
Christian Valcke
Joseph Niemann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanomix Inc
Original Assignee
Nanomix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/212,026 external-priority patent/US20070178477A1/en
Priority claimed from US11/274,747 external-priority patent/US20070208243A1/en
Application filed by Nanomix Inc filed Critical Nanomix Inc
Publication of EP1831670A2 publication Critical patent/EP1831670A2/en
Publication of EP1831670A4 publication Critical patent/EP1831670A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y10/00—Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4145—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/403—Cells and electrode assemblies
    • G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
    • G01N27/4146—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS involving nanosized elements, e.g. nanotubes, nanowires
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01L—SEMICONDUCTOR DEVICES NOT COVERED BY CLASS H10
    • H01L2924/00—Indexing scheme for arrangements or methods for connecting or disconnecting semiconductor or solid-state bodies as covered by H01L24/00
    • H01L2924/0001—Technical content checked by a classifier
    • H01L2924/0002—Not covered by any one of groups H01L24/00, H01L24/00 and H01L2224/00

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Pathology (AREA)
  • Nanotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Zoology (AREA)
  • Electrochemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Mathematical Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Fluid Adsorption Or Reactions (AREA)

Abstract

A nanotube device is configured as an electronic sensor for a target DNA sequence. A film of nanotubes is deposited over electrodes on a substrate. A solution of single-strand DNA is prepared so as to be complementary to a target DNA sequence. The DNA solution is deposited over the electrodes, dried, and removed from the substrate except in a region between the electrodes. The resulting structure includes strands of the desired DNA sequence in direct contact with nanotubes between opposing electrodes, to form a sensor that is electrically responsive to the presence of target DNA strands. Alternative assay embodiments are described which employ linker groups to attach ssDNA probes to the nanotube sensor device.
EP05855662A 2004-12-28 2005-12-23 Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences Withdrawn EP1831670A4 (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US63995404P 2004-12-28 2004-12-28
US65727505P 2005-02-28 2005-02-28
US66887905P 2005-04-05 2005-04-05
US11/212,026 US20070178477A1 (en) 2002-01-16 2005-08-24 Nanotube sensor devices for DNA detection
US73090505P 2005-10-27 2005-10-27
US11/274,747 US20070208243A1 (en) 2002-01-16 2005-11-15 Nanoelectronic glucose sensors
US73869405P 2005-11-21 2005-11-21
US74883405P 2005-12-09 2005-12-09
PCT/US2005/047143 WO2006071895A2 (en) 2004-12-28 2005-12-23 Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences

Publications (2)

Publication Number Publication Date
EP1831670A2 EP1831670A2 (en) 2007-09-12
EP1831670A4 true EP1831670A4 (en) 2010-09-15

Family

ID=36615493

Family Applications (1)

Application Number Title Priority Date Filing Date
EP05855662A Withdrawn EP1831670A4 (en) 2004-12-28 2005-12-23 Nanoelectronic devices for dna detection, and recognition of polynucleotide sequences

Country Status (3)

Country Link
EP (1) EP1831670A4 (en)
JP (1) JP2008525822A (en)
WO (1) WO2006071895A2 (en)

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US8154093B2 (en) 2002-01-16 2012-04-10 Nanomix, Inc. Nano-electronic sensors for chemical and biological analytes, including capacitance and bio-membrane devices
US7948041B2 (en) 2005-05-19 2011-05-24 Nanomix, Inc. Sensor having a thin-film inhibition layer
EP1781771A2 (en) * 2004-08-24 2007-05-09 Nanomix, Inc. Nanotube sensor devices for dna detection
JP2009532064A (en) * 2006-04-04 2009-09-10 ナノミックス・インコーポレーテッド Nanoelectronic detection of biomolecules using analyte amplification and reporter
ES2289948B1 (en) * 2006-07-19 2008-09-16 Starlab Barcelona, S.L. ELECTROPHYSIOLOGICAL SENSOR.
JP5061342B2 (en) * 2006-09-11 2012-10-31 国立大学法人大阪大学 Carbon nanotube electrode and sensor using the electrode
EP2160612A4 (en) * 2007-05-23 2016-04-27 Univ Arizona SYSTEMS AND METHODS FOR INTEGRATED ELECTROCHEMICAL AND ELECTRICAL DETECTION
WO2009026729A1 (en) * 2007-08-30 2009-03-05 University Of Waterloo Amino acid pairing-based self assembling peptides and methods
WO2010005080A1 (en) * 2008-07-10 2010-01-14 株式会社イデアルスター Gas sensor
US9919922B2 (en) 2008-10-02 2018-03-20 Saion Kumar Sinha Bionanosensor detection device
GB0822733D0 (en) * 2008-12-12 2009-01-21 Univ Warwick Nanotube electrochemistry
CN102471051B (en) 2009-08-07 2014-06-11 纳诺米克斯公司 Magnetic carbon nanotube based biodetection
JP2011220803A (en) * 2010-04-08 2011-11-04 Mitsumi Electric Co Ltd Biosensor including field-effect transistor element
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
AU2011312218B2 (en) 2010-10-04 2015-11-05 Sequencing Health, Inc. Systems and methods for automated reusable parallel biological reactions
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
JP5982472B2 (en) * 2011-05-27 2016-08-31 ジナプシス インコーポレイテッド Systems and methods for genetic and biological analysis
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
US10093975B2 (en) 2011-12-01 2018-10-09 Genapsys, Inc. Systems and methods for high efficiency electronic sequencing and detection
BR112015008206B1 (en) * 2012-10-16 2020-09-29 Koninklijke Philips N.V. INTEGRATED SENSOR CIRCUIT, SENSOR APPARATUS AND METHOD OF MEASURING A SUBSTANCE OF INTEREST TO BE ANALYZED IN A MEDIUM
CN102938397B (en) * 2012-12-05 2015-09-09 苏州纳格光电科技有限公司 Electronic device and preparation method thereof
CA2896879C (en) 2013-03-15 2020-09-22 Genapsys, Inc. Systems and methods for biological analysis
DE112014002575T5 (en) * 2013-05-29 2016-03-10 Csir Field effect transistor and a plurality of field effect transistors comprehensive gas detector
WO2015089238A1 (en) 2013-12-11 2015-06-18 Genapsys, Inc. Systems and methods for biological analysis and computation
CN105980581B (en) * 2013-12-12 2021-03-26 阿尔查技术有限公司 Capacitive sensor and method of use
EP3132060B1 (en) 2014-04-18 2019-03-13 Genapsys Inc. Methods and systems for nucleic acid amplification
US20180133679A1 (en) * 2015-04-15 2018-05-17 Alastair J. Malcolm Methods of manufacturing semiconductor arrays
WO2017033922A1 (en) * 2015-08-24 2017-03-02 国立大学法人 東京大学 Method of detecting target substance using field-effect transistor
KR102784420B1 (en) * 2016-01-14 2025-03-19 로스웰 엠이 아이엔씨. Molecular sensors and related methods
EP3488017A4 (en) 2016-07-20 2020-02-26 Genapsys Inc. SYSTEMS AND METHODS FOR NUCLEIC ACID SEQUENCING
SG11202002516WA (en) 2017-09-21 2020-04-29 Genapsys Inc Systems and methods for nucleic acid sequencing
US20190219512A1 (en) * 2018-01-12 2019-07-18 Clifford H. Kern, III Device, procedure and system for detecting bacterial pathogens including methicillin-resistant staphylococcus aureus or clostridium difficile
CN113588605B (en) * 2021-06-18 2024-08-06 山东师范大学 Method for detecting estrogen in multiple environments and application thereof
CA3223978A1 (en) * 2021-06-23 2022-12-29 Taimur Ahmed Conductometric sensor for detecting a nucleic acid and a method for the detection thereof
US11845079B2 (en) * 2021-12-08 2023-12-19 International Business Machines Corporation Integrated silicon platform for electronic biosensors

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JP2003322653A (en) * 2002-05-07 2003-11-14 Toshiba Corp Probe fixed support and probe fixed carrier
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Title
No further relevant documents disclosed *

Also Published As

Publication number Publication date
JP2008525822A (en) 2008-07-17
EP1831670A2 (en) 2007-09-12
WO2006071895A3 (en) 2007-02-08
WO2006071895A2 (en) 2006-07-06

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Legal Events

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PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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Effective date: 20070619

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DAX Request for extension of the european patent (deleted)
RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: NANOMIX, INC.

A4 Supplementary search report drawn up and despatched

Effective date: 20100818

RIC1 Information provided on ipc code assigned before grant

Ipc: C12M 3/00 20060101ALI20100812BHEP

Ipc: G01N 27/00 20060101ALI20100812BHEP

Ipc: G01N 27/12 20060101ALI20100812BHEP

Ipc: G01N 21/00 20060101AFI20070709BHEP

Ipc: G01N 33/543 20060101ALI20100812BHEP

Ipc: C12Q 1/68 20060101ALI20100812BHEP

Ipc: G01N 33/00 20060101ALI20100812BHEP

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