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EP2834649A4 - Cell culture and gradient migration assay methods and devices - Google Patents

Cell culture and gradient migration assay methods and devices

Info

Publication number
EP2834649A4
EP2834649A4 EP13772405.0A EP13772405A EP2834649A4 EP 2834649 A4 EP2834649 A4 EP 2834649A4 EP 13772405 A EP13772405 A EP 13772405A EP 2834649 A4 EP2834649 A4 EP 2834649A4
Authority
EP
European Patent Office
Prior art keywords
devices
cell culture
assay methods
migration assay
gradient migration
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP13772405.0A
Other languages
German (de)
French (fr)
Other versions
EP2834649A1 (en
Inventor
Ju-Sung Paul Hung
Philip J Lee
Amedeo J Cappione
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EMD Millipore Corp
Original Assignee
EMD Millipore Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US13/436,992 external-priority patent/US9637715B2/en
Application filed by EMD Millipore Corp filed Critical EMD Millipore Corp
Publication of EP2834649A1 publication Critical patent/EP2834649A1/en
Publication of EP2834649A4 publication Critical patent/EP2834649A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/46Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/16Microfluidic devices; Capillary tubes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484Optical investigation techniques, e.g. flow cytometry microstructural devices
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5029Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0809Geometry, shape and general structure rectangular shaped
    • B01L2300/0829Multi-well plates; Microtitration plates
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867Multiple inlets and one sample wells, e.g. mixing, dilution
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877Flow chambers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406Moving fluids with specific forces or mechanical means specific forces capillary forces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0457Moving fluids with specific forces or mechanical means specific forces passive flow or gravitation
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N2015/1006Investigating individual particles for cytology
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N2015/1027Determining speed or velocity of a particle

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Clinical Laboratory Science (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Sustainable Development (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
EP13772405.0A 2012-04-01 2013-02-06 Cell culture and gradient migration assay methods and devices Withdrawn EP2834649A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13/436,992 US9637715B2 (en) 2005-07-07 2012-04-01 Cell culture and invasion assay method and system
US201361761227P 2013-02-05 2013-02-05
PCT/US2013/024999 WO2013151616A1 (en) 2012-04-01 2013-02-06 Cell culture and gradient migration assay methods and devices

Publications (2)

Publication Number Publication Date
EP2834649A1 EP2834649A1 (en) 2015-02-11
EP2834649A4 true EP2834649A4 (en) 2015-12-09

Family

ID=49300898

Family Applications (1)

Application Number Title Priority Date Filing Date
EP13772405.0A Withdrawn EP2834649A4 (en) 2012-04-01 2013-02-06 Cell culture and gradient migration assay methods and devices

Country Status (5)

Country Link
EP (1) EP2834649A4 (en)
JP (2) JP2015517804A (en)
CN (2) CN104412109A (en)
SG (3) SG11201405210QA (en)
WO (1) WO2013151616A1 (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2992206B1 (en) * 2012-06-21 2014-07-18 Oreal COSMETIC COMPOSITION COMPRISING AN OIL, HYDROPHOBIC SILICA AEROGEL PARTICLES AND A HYDROCARBON RESIN
CA2901940A1 (en) 2013-02-22 2014-08-28 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
CA2901950A1 (en) 2013-02-22 2014-08-28 Genzyme Corporation Microcarrier perfusion culturing methods and uses thereof
JP6486898B2 (en) * 2014-02-25 2019-03-20 国立大学法人京都大学 Microfluidic device and micro three-dimensional culture method of cells
TWI743024B (en) 2014-06-06 2021-10-21 美商健臻公司 Perfusion culturing methods and uses thereof
TWI707949B (en) 2014-06-09 2020-10-21 美商健臻公司 Seed train processes and uses thereof
CA3225136A1 (en) 2014-12-22 2016-06-30 Genzyme Corporation Methods of culturing a mammalian cell
CN105062879B (en) * 2015-07-26 2017-06-27 江苏大学附属医院 Device for multi-directional cell migration experiment
CN105062890A (en) * 2015-09-11 2015-11-18 温州梅塔光学科技有限公司 Single cell experiment device
NL2016404B1 (en) 2016-03-09 2017-09-26 Mimetas B V Double tubular structures.
US11629319B2 (en) 2016-06-15 2023-04-18 Mimetas, B.V. Cell culture device and methods
WO2018001767A1 (en) * 2016-06-29 2018-01-04 Miltenyi Biotec Gmbh Multilevel disposable cartridge for biological specimens
WO2018234163A1 (en) * 2017-06-16 2018-12-27 İni̇ti̇o Biyomedikal Mühendislik Danişmanlik Sanayi Ve Ticaret Limited Şirketi METHOD FOR DETERMINING CELL MIGRATION AND INVASION
JP6812944B2 (en) * 2017-10-18 2021-01-13 株式会社島津製作所 Information management device for mass spectrometer
EP3755788A4 (en) * 2018-02-19 2021-12-22 National Research Council of Canada MICROFLUIDIC DEVICE FOR CULTIVATION OF CELLS WITH A BIOWALL, A BEAD BED AND A BIOS INTERFACE AND METHOD FOR MODELING THIS BIOS INTERFACE
NL2020518B1 (en) 2018-03-02 2019-09-12 Mimetas B V Device and method for performing electrical measurements
CN108641931A (en) * 2018-04-04 2018-10-12 浙江大学 A kind of digitlization microarray organ chip and its application
CN111057639B (en) * 2019-11-29 2022-06-17 中国医科大学 A system and method for the separation and enrichment of circulating tumor cells based on a microfluidic chip
JP7464123B2 (en) * 2020-07-06 2024-04-09 株式会社ニコン Migration calculation device, migration evaluation method, and computer program for causing a computer to execute the migration evaluation method
WO2022018743A1 (en) * 2020-07-22 2022-01-27 INDIAN INSTITUTE OF TECHNOLOGY MADRAS (IIT Madras) A system and method for simultaneous live-cell imaging and growing
CN112646713A (en) * 2020-12-25 2021-04-13 中国科学院广州生物医药与健康研究院 Chip for integrated tumor cell behavior experiment
NL2028424B1 (en) 2021-06-10 2022-12-20 Mimetas B V Method and apparatus for forming a microfluidic gel structure
CN113648287B (en) * 2021-07-30 2023-05-05 深圳华源再生医学有限公司 Cell loading device and preparation method thereof
CN116920974A (en) * 2023-08-16 2023-10-24 南方科技大学 A micropump-based sweat collection microfluidic patch and its preparation method
CN118496992B (en) * 2024-06-07 2025-01-24 北京大学 Device and method for high-throughput perfusion culture and imaging of pancreatic islets

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012024646A2 (en) * 2010-08-19 2012-02-23 The Johns Hopkins University Cell culture system and method of use thereof

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AU2003268201A1 (en) * 2002-08-27 2004-03-19 Vanderbilt University Capillary perfused bioreactors with multiple chambers
AU2004299530A1 (en) * 2003-12-19 2005-06-30 University Of Waterloo Cultured cell and method and apparatus for cell culture
JP2008519598A (en) * 2004-11-11 2008-06-12 エージェンシー フォー サイエンス,テクノロジー アンド リサーチ Cell culture devices
US9388374B2 (en) * 2005-07-07 2016-07-12 Emd Millipore Corporation Microfluidic cell culture systems
EP2214828A1 (en) * 2007-11-07 2010-08-11 Bellbrook Labs, Llc Microfluidic device having stable static gradient for analyzing chemotaxis

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012024646A2 (en) * 2010-08-19 2012-02-23 The Johns Hopkins University Cell culture system and method of use thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHENG SHING-YI ET AL: "A hydrogel-based microfluidic device for the studies of directed cell migration", LAB ON A CHIP, ROYAL SOCIETY OF CHEMISTRY - CAMBRIDGE, GB, vol. 7, no. 6, 1 June 2007 (2007-06-01), pages 763 - 769, XP002497558, ISSN: 1473-0197, [retrieved on 20070404], DOI: 10.1039/B618463D *
See also references of WO2013151616A1 *
SEOK CHUNG ET AL: "Cell migration into scaffolds under co-culture conditions in a microfluidic platform", LAB ON A CHIP, vol. 9, no. 2, 1 January 2009 (2009-01-01), pages 269, XP055066343, ISSN: 1473-0197, DOI: 10.1039/b807585a *

Also Published As

Publication number Publication date
CN104412109A (en) 2015-03-11
SG11201405210QA (en) 2014-09-26
JP2017093467A (en) 2017-06-01
JP2015517804A (en) 2015-06-25
SG10201803961VA (en) 2018-07-30
SG10201607963SA (en) 2016-11-29
EP2834649A1 (en) 2015-02-11
WO2013151616A1 (en) 2013-10-10
CN106399094A (en) 2017-02-15

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