EP2834649A4 - Cell culture and gradient migration assay methods and devices - Google Patents
Cell culture and gradient migration assay methods and devicesInfo
- Publication number
- EP2834649A4 EP2834649A4 EP13772405.0A EP13772405A EP2834649A4 EP 2834649 A4 EP2834649 A4 EP 2834649A4 EP 13772405 A EP13772405 A EP 13772405A EP 2834649 A4 EP2834649 A4 EP 2834649A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- devices
- cell culture
- assay methods
- migration assay
- gradient migration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000004113 cell culture Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 238000010232 migration assay Methods 0.000 title 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/46—Means for regulation, monitoring, measurement or control, e.g. flow regulation of cellular or enzymatic activity or functionality, e.g. cell viability
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
- G01N33/5029—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell motility
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0829—Multi-well plates; Microtitration plates
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0877—Flow chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0457—Moving fluids with specific forces or mechanical means specific forces passive flow or gravitation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1027—Determining speed or velocity of a particle
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Clinical Laboratory Science (AREA)
- Pathology (AREA)
- Genetics & Genomics (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Sustainable Development (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13/436,992 US9637715B2 (en) | 2005-07-07 | 2012-04-01 | Cell culture and invasion assay method and system |
| US201361761227P | 2013-02-05 | 2013-02-05 | |
| PCT/US2013/024999 WO2013151616A1 (en) | 2012-04-01 | 2013-02-06 | Cell culture and gradient migration assay methods and devices |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP2834649A1 EP2834649A1 (en) | 2015-02-11 |
| EP2834649A4 true EP2834649A4 (en) | 2015-12-09 |
Family
ID=49300898
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP13772405.0A Withdrawn EP2834649A4 (en) | 2012-04-01 | 2013-02-06 | Cell culture and gradient migration assay methods and devices |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP2834649A4 (en) |
| JP (2) | JP2015517804A (en) |
| CN (2) | CN104412109A (en) |
| SG (3) | SG11201405210QA (en) |
| WO (1) | WO2013151616A1 (en) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2992206B1 (en) * | 2012-06-21 | 2014-07-18 | Oreal | COSMETIC COMPOSITION COMPRISING AN OIL, HYDROPHOBIC SILICA AEROGEL PARTICLES AND A HYDROCARBON RESIN |
| CA2901940A1 (en) | 2013-02-22 | 2014-08-28 | Genzyme Corporation | Microcarrier perfusion culturing methods and uses thereof |
| CA2901950A1 (en) | 2013-02-22 | 2014-08-28 | Genzyme Corporation | Microcarrier perfusion culturing methods and uses thereof |
| JP6486898B2 (en) * | 2014-02-25 | 2019-03-20 | 国立大学法人京都大学 | Microfluidic device and micro three-dimensional culture method of cells |
| TWI743024B (en) | 2014-06-06 | 2021-10-21 | 美商健臻公司 | Perfusion culturing methods and uses thereof |
| TWI707949B (en) | 2014-06-09 | 2020-10-21 | 美商健臻公司 | Seed train processes and uses thereof |
| CA3225136A1 (en) | 2014-12-22 | 2016-06-30 | Genzyme Corporation | Methods of culturing a mammalian cell |
| CN105062879B (en) * | 2015-07-26 | 2017-06-27 | 江苏大学附属医院 | Device for multi-directional cell migration experiment |
| CN105062890A (en) * | 2015-09-11 | 2015-11-18 | 温州梅塔光学科技有限公司 | Single cell experiment device |
| NL2016404B1 (en) | 2016-03-09 | 2017-09-26 | Mimetas B V | Double tubular structures. |
| US11629319B2 (en) | 2016-06-15 | 2023-04-18 | Mimetas, B.V. | Cell culture device and methods |
| WO2018001767A1 (en) * | 2016-06-29 | 2018-01-04 | Miltenyi Biotec Gmbh | Multilevel disposable cartridge for biological specimens |
| WO2018234163A1 (en) * | 2017-06-16 | 2018-12-27 | İni̇ti̇o Biyomedikal Mühendislik Danişmanlik Sanayi Ve Ticaret Limited Şirketi | METHOD FOR DETERMINING CELL MIGRATION AND INVASION |
| JP6812944B2 (en) * | 2017-10-18 | 2021-01-13 | 株式会社島津製作所 | Information management device for mass spectrometer |
| EP3755788A4 (en) * | 2018-02-19 | 2021-12-22 | National Research Council of Canada | MICROFLUIDIC DEVICE FOR CULTIVATION OF CELLS WITH A BIOWALL, A BEAD BED AND A BIOS INTERFACE AND METHOD FOR MODELING THIS BIOS INTERFACE |
| NL2020518B1 (en) | 2018-03-02 | 2019-09-12 | Mimetas B V | Device and method for performing electrical measurements |
| CN108641931A (en) * | 2018-04-04 | 2018-10-12 | 浙江大学 | A kind of digitlization microarray organ chip and its application |
| CN111057639B (en) * | 2019-11-29 | 2022-06-17 | 中国医科大学 | A system and method for the separation and enrichment of circulating tumor cells based on a microfluidic chip |
| JP7464123B2 (en) * | 2020-07-06 | 2024-04-09 | 株式会社ニコン | Migration calculation device, migration evaluation method, and computer program for causing a computer to execute the migration evaluation method |
| WO2022018743A1 (en) * | 2020-07-22 | 2022-01-27 | INDIAN INSTITUTE OF TECHNOLOGY MADRAS (IIT Madras) | A system and method for simultaneous live-cell imaging and growing |
| CN112646713A (en) * | 2020-12-25 | 2021-04-13 | 中国科学院广州生物医药与健康研究院 | Chip for integrated tumor cell behavior experiment |
| NL2028424B1 (en) | 2021-06-10 | 2022-12-20 | Mimetas B V | Method and apparatus for forming a microfluidic gel structure |
| CN113648287B (en) * | 2021-07-30 | 2023-05-05 | 深圳华源再生医学有限公司 | Cell loading device and preparation method thereof |
| CN116920974A (en) * | 2023-08-16 | 2023-10-24 | 南方科技大学 | A micropump-based sweat collection microfluidic patch and its preparation method |
| CN118496992B (en) * | 2024-06-07 | 2025-01-24 | 北京大学 | Device and method for high-throughput perfusion culture and imaging of pancreatic islets |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012024646A2 (en) * | 2010-08-19 | 2012-02-23 | The Johns Hopkins University | Cell culture system and method of use thereof |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2003268201A1 (en) * | 2002-08-27 | 2004-03-19 | Vanderbilt University | Capillary perfused bioreactors with multiple chambers |
| AU2004299530A1 (en) * | 2003-12-19 | 2005-06-30 | University Of Waterloo | Cultured cell and method and apparatus for cell culture |
| JP2008519598A (en) * | 2004-11-11 | 2008-06-12 | エージェンシー フォー サイエンス,テクノロジー アンド リサーチ | Cell culture devices |
| US9388374B2 (en) * | 2005-07-07 | 2016-07-12 | Emd Millipore Corporation | Microfluidic cell culture systems |
| EP2214828A1 (en) * | 2007-11-07 | 2010-08-11 | Bellbrook Labs, Llc | Microfluidic device having stable static gradient for analyzing chemotaxis |
-
2013
- 2013-02-06 SG SG11201405210QA patent/SG11201405210QA/en unknown
- 2013-02-06 SG SG10201607963SA patent/SG10201607963SA/en unknown
- 2013-02-06 CN CN201380018324.1A patent/CN104412109A/en active Pending
- 2013-02-06 EP EP13772405.0A patent/EP2834649A4/en not_active Withdrawn
- 2013-02-06 WO PCT/US2013/024999 patent/WO2013151616A1/en active Application Filing
- 2013-02-06 JP JP2015503203A patent/JP2015517804A/en active Pending
- 2013-02-06 CN CN201610259619.3A patent/CN106399094A/en active Pending
- 2013-02-06 SG SG10201803961VA patent/SG10201803961VA/en unknown
-
2017
- 2017-02-06 JP JP2017019804A patent/JP2017093467A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012024646A2 (en) * | 2010-08-19 | 2012-02-23 | The Johns Hopkins University | Cell culture system and method of use thereof |
Non-Patent Citations (3)
| Title |
|---|
| CHENG SHING-YI ET AL: "A hydrogel-based microfluidic device for the studies of directed cell migration", LAB ON A CHIP, ROYAL SOCIETY OF CHEMISTRY - CAMBRIDGE, GB, vol. 7, no. 6, 1 June 2007 (2007-06-01), pages 763 - 769, XP002497558, ISSN: 1473-0197, [retrieved on 20070404], DOI: 10.1039/B618463D * |
| See also references of WO2013151616A1 * |
| SEOK CHUNG ET AL: "Cell migration into scaffolds under co-culture conditions in a microfluidic platform", LAB ON A CHIP, vol. 9, no. 2, 1 January 2009 (2009-01-01), pages 269, XP055066343, ISSN: 1473-0197, DOI: 10.1039/b807585a * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104412109A (en) | 2015-03-11 |
| SG11201405210QA (en) | 2014-09-26 |
| JP2017093467A (en) | 2017-06-01 |
| JP2015517804A (en) | 2015-06-25 |
| SG10201803961VA (en) | 2018-07-30 |
| SG10201607963SA (en) | 2016-11-29 |
| EP2834649A1 (en) | 2015-02-11 |
| WO2013151616A1 (en) | 2013-10-10 |
| CN106399094A (en) | 2017-02-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
| 17P | Request for examination filed |
Effective date: 20140913 |
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| AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR |
|
| AX | Request for extension of the european patent |
Extension state: BA ME |
|
| DAX | Request for extension of the european patent (deleted) | ||
| RA4 | Supplementary search report drawn up and despatched (corrected) |
Effective date: 20151106 |
|
| RIC1 | Information provided on ipc code assigned before grant |
Ipc: C12M 1/18 20060101ALI20151102BHEP Ipc: C12Q 1/02 20060101ALI20151102BHEP Ipc: G01N 35/08 20060101AFI20151102BHEP |
|
| 17Q | First examination report despatched |
Effective date: 20190122 |
|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
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| 18D | Application deemed to be withdrawn |
Effective date: 20190402 |