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EP4419886A1 - A composition, method and kit for extracting a narcotic compound from a biological sample - Google Patents

A composition, method and kit for extracting a narcotic compound from a biological sample

Info

Publication number
EP4419886A1
EP4419886A1 EP21961285.0A EP21961285A EP4419886A1 EP 4419886 A1 EP4419886 A1 EP 4419886A1 EP 21961285 A EP21961285 A EP 21961285A EP 4419886 A1 EP4419886 A1 EP 4419886A1
Authority
EP
European Patent Office
Prior art keywords
composition
sample
liquid mixture
weight
tube
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP21961285.0A
Other languages
German (de)
French (fr)
Other versions
EP4419886A4 (en
Inventor
Ebtisam ABDULRAHMAN SALEH ALABDOOLI
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dubai Police
Original Assignee
Dubai Police
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dubai Police filed Critical Dubai Police
Publication of EP4419886A1 publication Critical patent/EP4419886A1/en
Publication of EP4419886A4 publication Critical patent/EP4419886A4/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4055—Concentrating samples by solubility techniques
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B01D11/0288—Applications, solvents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/04—Solvent extraction of solutions which are liquid
    • B01D11/0492—Applications, solvents used
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4055—Concentrating samples by solubility techniques
    • G01N2001/4061—Solvent extraction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2560/00—Chemical aspects of mass spectrometric analysis of biological material

Definitions

  • This invention relates to extraction of narcotic compounds from a sample, in particular this invention relates to a novel composition, method and kit including the novel composition for extracting narcotic compounds from a biological sample. background
  • LLE liquid/liquid extractions
  • a composition for extraction of narcotic compounds from a sample including a first liquid mixture and second solid composition: - the first liquid mixture having, a source of heptane, either n-heptane or heptane having mixed isomers and/or enantiomers thereof comprising from about 10% to about 25%, by weight of the liquid mixture, an organochloride compound being dichloromethane comprising from about 25% to about 35%, by weight of the liquid mixture, a chlorinated hydrocarbon being 1 ,2-Dichloroethane comprising from about 30% to about 45%, by weight of the liquid mixture, a source of liquid propane comprising from about 5% to about 21 %, by weight of the liquid mixture, the second solid composition; having a source of sodium chloride, comprising from about 16% to about 63 %, by weight of the second solid composition, a source of sodium carbonate, comprising from about 17 % to about 34 %, by weight of the second solid composition, a
  • the final composition may have a pH of 9.
  • the source of heptane may have a volume of between 1 and 1 .4 micro litre.
  • the dichloro methane solvent may have a volume of between 0.7 and 2 micro litre.
  • the dichloro ethane solvent may have a volume of between 0.7 and 4 micro litre.
  • the liquid propane may have a volume of between 0.5 and 4 micro litre.
  • the sodium chloride may have a mass of between 0.5 and 1 grams. In one formulation the sodium carbonate may have a mass of between 0.15 and 1 grams.
  • the sodium bi carbonate may have a mass of between 0.15 and 1 grams.
  • the sodium chloride, sodium carbonate and sodium bi-carbonate may be in the form of a powder.
  • the sample may be in the form of any one or more of the group including a biological sample, a petroleum sample, an ink sample, a dye sample, an organic sample or the like.
  • the biological sample may be selected from the group including blood, urine, tissue, stomach contents or the like.
  • a kit for extraction of narcotic compounds from a sample including: - a composition having a first liquid mixture and second solid composition as hereinbefore described; and a tube which is configured to contain the composition therein and further be configured to receive a sample for extraction of narcotic compounds from the sample.
  • the sample may be in the form of any one or more of the group including a biological sample, a petroleum sample, an ink sample, a dye sample, an organic sample or the like.
  • the tube may be a pathology tube.
  • the biological sample may be selected from the group including blood, urine, tissue, stomach contents or the like.
  • a method for extraction of narcotic compounds from a sample including the following steps: providing a tube having a composition therein, said composition having a first liquid mixture and second solid composition as hereinbefore described; and adding a desired biological sample to the tube.
  • the sample may be a 5ml urine sample.
  • An additional step may include vortexing the tube for at least 2 minutes.
  • Yet another step may include centrifuging the tube for at least 3 minutes at a speed of at least 4000 rpm.
  • a still further step may include additional centrifuging of the tube for another 3 minutes at a speed of at least 4000 rpm.
  • a still further step may include transferring the upper layer of the fluid inside the tube and evaporating said upper layer at 40 degrees Celsius under nitrogen.
  • a further step may include re-constitution of the residue using 50 micro litres of ethyl acetate.
  • a further step may include extracting 2 micro litres of extract and injecting said extract into the confirmatory instrument.
  • the confirmatory instrument may be a gas chromatography and mass spectrometry (GC-MS).
  • Figure 1 is a perspective view of a kit for extraction of narcotic compounds from a sample, in accordance with a second aspect of the invention, including a composition having a first liquid mixture and second solid composition as described in accordance with a first aspect of the invention;
  • Figure 2 is another perspective view of a kit as shown in Figure 1 , wherein the first liquid mixture and second solid composition has been mixed to form a final composition;
  • Figure 3 is another perspective view of a kit as shown in Figure 2, wherein, is use, a sample is added to the final composition;
  • Figure 4 is a schematic showing a flow diagram of a method in accordance with a third aspect of the invention.
  • a composition for extraction of narcotic compounds from a sample including a first liquid mixture and second solid composition, the first liquid mixture having, a source of heptane, either n-heptane or heptane having mixed isomers and/or enantiomers thereof comprising from about 10% to about 25%, by weight of the liquid mixture, an organochloride compound being dichloromethane comprising from about 25% to about 35%, by weight of the liquid mixture, a chlorinated hydrocarbon being 1 ,2- Dichloroethane comprising from about 30% to about 45%, by weight of the liquid mixture, a source of liquid propane comprising from about 5% to about 21 %, by weight of the liquid mixture, the second solid composition, having a source of sodium chloride, comprising from about 16% to about 63 %, by weight of the second solid composition, a source of sodium carbonate, comprising from about 17 % to about 34 %, by weight of the second solid composition,
  • the final composition has a pH of 9.
  • the source of heptane has a volume of between 1 and 1 .4 micro litre.
  • the dichloro methane solvent has a volume of between 0.7 and 2 micro litre.
  • the dichloro ethane solvent has a volume of between 0.7 and 4 micro litre.
  • the liquid propane has a volume of between 0.5 and 4 micro litre.
  • the sodium chloride has a mass of between 0.5 and 1 grams.
  • the sodium carbonate has a mass of between 0.15 and 1 grams.
  • the sodium bi carbonate has a mass of between 0.15 and 1 grams.
  • the sodium chloride, sodium carbonate and sodium bi-carbonate is in the form of a powder.
  • the biological sample is selected from the group including blood, urine, tissue, stomach contents or the like.
  • kits 100 for extraction of narcotic compounds from a sample including a composition 1 10 having a first liquid mixture 114 and second solid composition 1 16 as hereinbefore described, and a tube 1 18 which is configured to contain the composition 1 10 therein and further be configured to receive a sample 1 12 for extraction of narcotic compounds from the sample 1 12.
  • the sample 1 12 is in the form of any one or more of the group including a biological sample, a petroleum sample, an ink sample, a dye sample, an organic sample or the like.
  • the biological sample 1 12 is selected from the group including blood, urine, tissue, stomach contents or the like.
  • a method 200 for extraction of narcotic compounds from a sample including the following steps, providing a tube 218 having a composition 210 therein, said composition 210 having a first liquid mixture 214 and second solid composition 216 as hereinbefore described, and adding a desired biological sample 212 to the tube 218.
  • the desired biological sample 212 is a 5ml urine sample.
  • Yet another step 202 includes centrifuging the tube 218 for at least 3 minutes at a speed of at least 4000 rpm.
  • a still further step 203 includes additional centrifuging of the tube 218 for another 3 minutes at a speed of at least 4000 rpm.
  • a still further step 204 includes transferring the upper layer of the fluid inside the tube 218 and evaporating said upper layer at 40 degrees Celsius under nitrogen.
  • a further step 205 includes re-constitution of the residue using 50 micro litres of ethyl acetate.
  • a further step 206 includes extracting 2 micro litres of extract and injecting said extract into the confirmatory instrument 220.
  • the confirmatory instrument 220 is a gas chromatography and mass spectrometry (GC-MS).

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A composition for extraction of narcotic compounds from a sample, said composition including a first liquid mixture and second solid composition, the first liquid mixture having, a source of heptane, either n-heptane or heptane having mixed isomers and/or enantiomers thereof comprising from about 10% to about 25%, by weight of the liquid mixture, an organochloride compound being dichloromethane comprising from about 25% to about 35%, by weight of the liquid mixture, a chlorinated hydrocarbon being 1,2-Dichloroethane comprising from about 30% to about 45%, by weight of the liquid mixture, a source of liquid propane comprising from about 5% to about 21%, by weight of the liquid mixture, the second solid composition, having a source of sodium chloride, comprising from about 16% to about 63 %, by weight of the second solid composition, a source of sodium carbonate, comprising from about 17 % to about 34 %, by weight of the second solid composition, a source of sodium bi carbonate, comprising from about 17% to about 50 %, by weight of the second solid composition, wherein the second solid composition is added to the first liquid mixture in sufficient amount to enable the final composition to have a pH of between 8 and 10.

Description

A COMPOSITION, METHOD AND KIT for extracting a NARCOTIC COMPOUND FROM A BIOLOGICAL SAMPLE
Technical field
This invention relates to extraction of narcotic compounds from a sample, in particular this invention relates to a novel composition, method and kit including the novel composition for extracting narcotic compounds from a biological sample. background
The extraction of narcotic compounds from biological matrices is an essential specimen preparation step in current forensic laboratories. Traditionally, liquid/liquid extractions (LLE) were developed and employed to screen for the general unknown as LLE are generally known in the art as useful for separating components of a mixture, wherein the constituents have differing polarities which can be separated when mixed within two immiscible solvents that form a liquid bilayer after mixing.
The expert in the field of forensic toxicology and chemistry cannot use any advance instrument such as gas chromatography and mass spectrometry without the step of preparation or extraction of the samples to get predictable compounds that entered the human body and further quantification is extremely difficult, hence extraction and preparation of the biological samples is considered as the backbone of the work in the toxicology and chemistry field anywhere in the world.
However, there is a general lack of information pertaining to the correct use and type of solvents used for extraction and this poses a serious problem as forensic laboratories cannot easily determine which is the best solvents needed for biological samples and conventional methods are time consuming, complex and costly. Moreover, conventional solvents used in the extraction do not cover all types of narcotic substances that are searched for in samples, as it is common cause that type of narcotic drugs tend to differ according to geographical area, resulting in either a plurality of tests or different methods to reach satisfactory results.
Furthermore, procurement problems are usually associated with procurement of certain types of solvents, said problems compounded with shelf lifetime restraints and difficulty in actual importation of certain types of solvents.
It is an object of the present invention to alleviate at least some of the disadvantages mentioned above. summary of the invention
According to the invention, there is provided a composition for extraction of narcotic compounds from a sample, said composition including a first liquid mixture and second solid composition: - the first liquid mixture having, a source of heptane, either n-heptane or heptane having mixed isomers and/or enantiomers thereof comprising from about 10% to about 25%, by weight of the liquid mixture, an organochloride compound being dichloromethane comprising from about 25% to about 35%, by weight of the liquid mixture, a chlorinated hydrocarbon being 1 ,2-Dichloroethane comprising from about 30% to about 45%, by weight of the liquid mixture, a source of liquid propane comprising from about 5% to about 21 %, by weight of the liquid mixture, the second solid composition; having a source of sodium chloride, comprising from about 16% to about 63 %, by weight of the second solid composition, a source of sodium carbonate, comprising from about 17 % to about 34 %, by weight of the second solid composition, a source of sodium bi carbonate, comprising from about 17% to about 50 %, by weight of the second solid composition, wherein the second solid composition is added to the first liquid mixture in sufficient amount to enable a final composition to have a pH of between 8 and 10.
In one formulation the final composition may have a pH of 9.
In one formulation the source of heptane may have a volume of between 1 and 1 .4 micro litre.
In one formulation the dichloro methane solvent may have a volume of between 0.7 and 2 micro litre.
In one formulation the dichloro ethane solvent may have a volume of between 0.7 and 4 micro litre.
In one formulation the liquid propane may have a volume of between 0.5 and 4 micro litre.
In one formulation the sodium chloride may have a mass of between 0.5 and 1 grams. In one formulation the sodium carbonate may have a mass of between 0.15 and 1 grams.
The sodium bi carbonate may have a mass of between 0.15 and 1 grams.
In yet another formulation the sodium chloride, sodium carbonate and sodium bi-carbonate may be in the form of a powder.
The sample may be in the form of any one or more of the group including a biological sample, a petroleum sample, an ink sample, a dye sample, an organic sample or the like.
The biological sample may be selected from the group including blood, urine, tissue, stomach contents or the like.
According to a second aspect of the invention, there is provided a kit for extraction of narcotic compounds from a sample including: - a composition having a first liquid mixture and second solid composition as hereinbefore described; and a tube which is configured to contain the composition therein and further be configured to receive a sample for extraction of narcotic compounds from the sample.
The sample may be in the form of any one or more of the group including a biological sample, a petroleum sample, an ink sample, a dye sample, an organic sample or the like.
The tube may be a pathology tube. The biological sample may be selected from the group including blood, urine, tissue, stomach contents or the like.
According to a third aspect of the invention, there is provided a method for extraction of narcotic compounds from a sample, said method including the following steps: providing a tube having a composition therein, said composition having a first liquid mixture and second solid composition as hereinbefore described; and adding a desired biological sample to the tube.
In one formulation the sample may be a 5ml urine sample.
An additional step may include vortexing the tube for at least 2 minutes.
Yet another step may include centrifuging the tube for at least 3 minutes at a speed of at least 4000 rpm.
A still further step may include additional centrifuging of the tube for another 3 minutes at a speed of at least 4000 rpm.
A still further step may include transferring the upper layer of the fluid inside the tube and evaporating said upper layer at 40 degrees Celsius under nitrogen.
A further step may include re-constitution of the residue using 50 micro litres of ethyl acetate. A further step may include extracting 2 micro litres of extract and injecting said extract into the confirmatory instrument.
In one formulation the confirmatory instrument may be a gas chromatography and mass spectrometry (GC-MS).
BRIEF DESCRIPTION OF THE DRAWINGS
A composition, method and kit for extracting a narcotic compound from a biological sample in accordance with the invention will now be described by way of the following, non-limiting examples with reference to the accompanying drawings.
In the drawings: -
Figure 1 is a perspective view of a kit for extraction of narcotic compounds from a sample, in accordance with a second aspect of the invention, including a composition having a first liquid mixture and second solid composition as described in accordance with a first aspect of the invention;
Figure 2 is another perspective view of a kit as shown in Figure 1 , wherein the first liquid mixture and second solid composition has been mixed to form a final composition;
Figure 3 is another perspective view of a kit as shown in Figure 2, wherein, is use, a sample is added to the final composition; and
Figure 4 is a schematic showing a flow diagram of a method in accordance with a third aspect of the invention.
Detailed description of the invention
According to a first aspect of the invention there is provided a composition for extraction of narcotic compounds from a sample, said composition including a first liquid mixture and second solid composition, the first liquid mixture having, a source of heptane, either n-heptane or heptane having mixed isomers and/or enantiomers thereof comprising from about 10% to about 25%, by weight of the liquid mixture, an organochloride compound being dichloromethane comprising from about 25% to about 35%, by weight of the liquid mixture, a chlorinated hydrocarbon being 1 ,2- Dichloroethane comprising from about 30% to about 45%, by weight of the liquid mixture, a source of liquid propane comprising from about 5% to about 21 %, by weight of the liquid mixture, the second solid composition, having a source of sodium chloride, comprising from about 16% to about 63 %, by weight of the second solid composition, a source of sodium carbonate, comprising from about 17 % to about 34 %, by weight of the second solid composition, a source of sodium bi carbonate, comprising from about 17% to about 50 %, by weight of the second solid composition, wherein the second solid composition is added to the first liquid mixture in sufficient amount to enable a final composition to have a pH of between 8 and 10.
In one formulation the final composition has a pH of 9.
The source of heptane has a volume of between 1 and 1 .4 micro litre.
The dichloro methane solvent has a volume of between 0.7 and 2 micro litre.
The dichloro ethane solvent has a volume of between 0.7 and 4 micro litre.
The liquid propane has a volume of between 0.5 and 4 micro litre.
The sodium chloride has a mass of between 0.5 and 1 grams.
The sodium carbonate has a mass of between 0.15 and 1 grams. The sodium bi carbonate has a mass of between 0.15 and 1 grams.
The sodium chloride, sodium carbonate and sodium bi-carbonate is in the form of a powder.
The sample is in the form of any one or more of the group including a biological sample, a petroleum sample, an ink sample, a dye sample, an organic sample or the like.
The biological sample is selected from the group including blood, urine, tissue, stomach contents or the like.
According to a second aspect of the invention, as shown in Figures 1 to 3, there is provided a kit 100 for extraction of narcotic compounds from a sample including a composition 1 10 having a first liquid mixture 114 and second solid composition 1 16 as hereinbefore described, and a tube 1 18 which is configured to contain the composition 1 10 therein and further be configured to receive a sample 1 12 for extraction of narcotic compounds from the sample 1 12.
The sample 1 12 is in the form of any one or more of the group including a biological sample, a petroleum sample, an ink sample, a dye sample, an organic sample or the like.
The tube 1 18 is a pathology tube.
The biological sample 1 12 is selected from the group including blood, urine, tissue, stomach contents or the like. As shown in Figure 4, according to a third aspect of the invention, there is provided a method 200 for extraction of narcotic compounds from a sample, said method including the following steps, providing a tube 218 having a composition 210 therein, said composition 210 having a first liquid mixture 214 and second solid composition 216 as hereinbefore described, and adding a desired biological sample 212 to the tube 218.
In one formulation the desired biological sample 212 is a 5ml urine sample.
The method 200 further includes the step 201 of vortexing the tube 218 for at least 2 minutes.
Yet another step 202 includes centrifuging the tube 218 for at least 3 minutes at a speed of at least 4000 rpm.
A still further step 203 includes additional centrifuging of the tube 218 for another 3 minutes at a speed of at least 4000 rpm.
A still further step 204 includes transferring the upper layer of the fluid inside the tube 218 and evaporating said upper layer at 40 degrees Celsius under nitrogen.
A further step 205 includes re-constitution of the residue using 50 micro litres of ethyl acetate.
A further step 206 includes extracting 2 micro litres of extract and injecting said extract into the confirmatory instrument 220. The confirmatory instrument 220 is a gas chromatography and mass spectrometry (GC-MS).
Although only certain formulations of the invention have been described herein, it will be understood by any person skilled in the art that other modifications, variations, and possibilities of the invention are possible. Such modifications, variations and possibilities are therefore to be considered as falling within the spirit and scope of the invention and hence form part of the invention as herein described and/or exemplified. It is further to be understood that the examples are provided for illustrating the invention further and to assist a person skilled in the art with understanding the invention and is not meant to be construed as unduly limiting the reasonable scope of the invention.
The inventor believes that the composition, method and kit for extracting a narcotic compound from a biological sample in accordance with the present invention is advantageous in that provides an easy, convenient and quick way to extract a narcotic compound from a biological sample. The invention is further advantageous in that it allows for reduced reliance on foreign products. Yet furthermore, the invention is advantageous in that it allows for a large variety and range of narcotic compounds to be extracted from a biological sample. The invention provides an extracted narcotic compound with a high degree of purity which allows the life of the advanced instrument, such as a gas and liquid chromatography and mass spectrometry, that used in the process of confirmatory tests to be extended.

Claims

claims
A composition for extraction of narcotic compounds from a sample, said composition including a first liquid mixture and second solid composition: - the first liquid mixture having, a source of heptane, either n-heptane or heptane having mixed isomers and/or enantiomers thereof comprising from about 10% to about 25%, by weight of the liquid mixture, an organochloride compound being dichloromethane comprising from about 25% to about 35%, by weight of the liquid mixture, a chlorinated hydrocarbon being 1 ,2-Dichloroethane comprising from about 30% to about 45%, by weight of the liquid mixture, a source of liquid propane comprising from about 5% to about 21 %, by weight of the liquid mixture, the second solid composition; having a source of sodium chloride, comprising from about 16% to about 63 %, by weight of the second solid composition, a source of sodium carbonate, comprising from about 17% to about 34 %, by weight of the second solid composition, a source of sodium bi carbonate, comprising from about 17% to about 50 %, by weight of the second solid composition, wherein the second solid composition is added to the first liquid mixture in sufficient amount to enable a final composition to have a pH of between 8 and 10.
A composition as claimed in claim 1 , wherein the final composition has a pH of 9.
A composition as claimed in claim 1 , wherein the source of heptane has a volume of between 1 and 1 .4 micro litre. A composition as claimed in claims 1 , wherein the dichloro methane solvent has a volume of between 0.7 and 2 micro litre.
A composition as claimed in claim 1 , wherein dichloro ethane solvent has a volume of between 0.7 and 4 micro litre.
A composition as claimed in claim 1 , wherein the liquid propane has a volume of between 0.5 and 4 micro litre.
A composition as claimed in claim 1 , wherein the sodium chloride has a mass of between 0.5 and 1 grams.
A composition as claimed in claim 1 , wherein the sodium carbonate has a mass of between 0.15 and 1 grams.
A composition as claimed in claim 1 , wherein the sodium bi carbonate has a mass of between 0.15 and 1 grams.
A composition as claimed in claim 1 , wherein the sodium chloride, sodium carbonate and sodium bi-carbonate is in the form of a powder.
A composition as claimed in claim 1 , wherein the sample is in the form of any one or more of the group including a biological sample, a petroleum sample, an ink sample, a dye sample, or an organic sample. A composition as claimed in claim 11 wherein the biological sample is selected from the group including blood, urine, tissue, or stomach contents.
A kit for extraction of narcotic compounds from a sample including: - a composition having a first liquid mixture and second solid composition as hereinbefore described; and a tube which is configured to contain the composition therein and further be configured to receive a sample for extraction of narcotic compounds from the sample.
A kit as claimed in claim 13 wherein the sample is in the form of any one or more of the group including a biological sample, a petroleum sample, an ink sample, a dye sample, or an organic sample.
A kit as claimed in claim 14 wherein the biological sample is selected from the group including blood, urine, tissue, or stomach contents.
A kit as claimed in claim 13, wherein the tube is a pathology tube.
A method for extraction of narcotic compounds from a sample, said method including the following steps: providing a tube having a composition therein, said composition having a first liquid mixture and second solid composition as hereinbefore described; and adding a desired biological sample to the tube.
A method as claimed in claim 17, wherein the desired biological sample is a 5ml urine sample. A method as claimed in claim 18 wherein said method includes the step of vortexing the tube for at least 2 minutes.
A method as claimed in claim 19 wherein said method includes the step of centrifuging the tube for at least 3 minutes at a speed of at least 4000 rpm.
A method as claimed in claim 20 wherein said method includes the step of additional centrifuging of the tube for another 3 minutes at a speed of at least 4000 rpm.
A method as claimed in claim 21 wherein said method includes the step of transferring the upper layer of the fluid inside the tube and evaporating said upper layer at 40 degrees Celsius under nitrogen.
A method as claimed in claim 22 wherein said method includes the step of reconstitution of the residue using 50 micro litres of ethyl acetate.
A method as claimed in claim 23 wherein said method includes the step of extracting 2 micro litres of extract and injecting said extract into the confirmatory instrument.
A method as claimed in claim 24 wherein the confirmatory instrument is a gas chromatography and mass spectrometry (GC-MS).
EP21961285.0A 2021-10-19 2021-10-19 Composition, method and kit for extracting a narcotic compound from a biological sample Pending EP4419886A4 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IB2021/059595 WO2023067370A1 (en) 2021-10-19 2021-10-19 A composition, method and kit for extracting a narcotic compound from a biological sample

Publications (2)

Publication Number Publication Date
EP4419886A1 true EP4419886A1 (en) 2024-08-28
EP4419886A4 EP4419886A4 (en) 2025-10-15

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Country Status (2)

Country Link
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WO (1) WO2023067370A1 (en)

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EP2687854A1 (en) * 2012-07-19 2014-01-22 Chiron AS Test kit for the quantitative determination of narcotic drugs
US9726684B1 (en) * 2015-01-18 2017-08-08 Hound Labs, Inc. Compositions for target substance detection and measurement
US9823259B1 (en) * 2016-04-08 2017-11-21 Walter Nichols Detection device for cannabinoid use
CN111868530B (en) * 2018-02-14 2024-06-11 萨琳格纳斯提克斯有限公司 Methods and devices for detecting analytes
KR102188845B1 (en) * 2018-10-08 2020-12-09 한국과학기술원 Colorimetric drug sensors with drug detecting color change dye anchored one dimensional nanofiber membrane and manufacturing method thereof

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