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FI112668B - Bacillus-promoottori, joka on peräisin Bacillus licheniformis x-amylaasipromoottorimuunnelmasta - Google Patents

Bacillus-promoottori, joka on peräisin Bacillus licheniformis x-amylaasipromoottorimuunnelmasta Download PDF

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Publication number
FI112668B
FI112668B FI942228A FI942228A FI112668B FI 112668 B FI112668 B FI 112668B FI 942228 A FI942228 A FI 942228A FI 942228 A FI942228 A FI 942228A FI 112668 B FI112668 B FI 112668B
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Finland
Prior art keywords
bacillus
promoter
sequence
eller
gene
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FI942228A
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English (en)
Finnish (fi)
Swedish (sv)
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FI942228L (fi
FI942228A0 (fi
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Steen Troels Joergensen
Boerge Krag Diderichsen
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Novozymes As
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Publication of FI942228A0 publication Critical patent/FI942228A0/fi
Publication of FI942228L publication Critical patent/FI942228L/fi
Application granted granted Critical
Publication of FI112668B publication Critical patent/FI112668B/fi

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source
    • CCHEMISTRY; METALLURGY
    • C11ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11DDETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16Organic compounds
    • C11D3/38Products with no well-defined composition, e.g. natural products
    • C11D3/386Preparations containing enzymes, e.g. protease or amylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • C12N9/1074Cyclomaltodextrin glucanotransferase (2.4.1.19)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Devices For Conveying Motion By Means Of Endless Flexible Members (AREA)
FI942228A 1991-11-14 1994-05-13 Bacillus-promoottori, joka on peräisin Bacillus licheniformis x-amylaasipromoottorimuunnelmasta FI112668B (fi)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DK9100343 1991-11-14
DK9100343 1991-11-14
PCT/DK1992/000338 WO1993010249A1 (fr) 1991-11-14 1992-11-13 Promoteur de bacillus derive d'un variant d'un promoteur de bacillus licheniformis x-amylase
DK9200338 1992-11-13

Publications (3)

Publication Number Publication Date
FI942228A0 FI942228A0 (fi) 1994-05-13
FI942228L FI942228L (fi) 1994-07-13
FI112668B true FI112668B (fi) 2003-12-31

Family

ID=8153698

Family Applications (1)

Application Number Title Priority Date Filing Date
FI942228A FI112668B (fi) 1991-11-14 1994-05-13 Bacillus-promoottori, joka on peräisin Bacillus licheniformis x-amylaasipromoottorimuunnelmasta

Country Status (11)

Country Link
US (1) US5698415A (fr)
EP (1) EP0667910B1 (fr)
JP (1) JP3529774B2 (fr)
AT (1) ATE248223T1 (fr)
AU (1) AU661070B2 (fr)
CA (1) CA2123496C (fr)
DE (1) DE69233178T2 (fr)
DK (1) DK0667910T3 (fr)
FI (1) FI112668B (fr)
IL (1) IL103750A0 (fr)
WO (1) WO1993010249A1 (fr)

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US5955310A (en) * 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
US6300115B1 (en) 1998-05-18 2001-10-09 Enzyme Bio-Systems Ltd. Pullulanase expression constructs containing α-amylase promoter and leader sequences
US7521204B2 (en) 2002-04-10 2009-04-21 Novozymes A/S Bacillus host cell
EP1696035A3 (fr) 2002-04-10 2008-04-02 Novozymes A/S Cellule hôte de bacille améliorée
WO2004101759A2 (fr) 2003-05-12 2004-11-25 Genencor International, Inc. Nouvelle enzyme lipolytique lip2
EP1625202A4 (fr) 2003-05-12 2010-10-20 Genencor Int Enzyme lipolytique lip1
WO2004111224A1 (fr) 2003-06-19 2004-12-23 Novozymes A/S Proteases ameliorees et procedes de production de ces dernieres
CN101426925A (zh) * 2004-03-31 2009-05-06 诺维信生物聚合物公司 在芽孢杆菌细胞中生产透明质酸的方法
WO2005123915A1 (fr) 2004-06-21 2005-12-29 Novozymes A/S Copies multiples d'au moins deux cadres de lecture ouverte maintenus stables dans la meme orientation
CN101611145B (zh) 2006-12-21 2013-08-14 诺维信股份有限公司 用于在细菌细胞中表达基因的修饰型信使rna稳定化序列
BRPI0809096A2 (pt) 2007-03-23 2014-09-09 Danisco Us Inc Genecor Division Produção aumentada de amilase através da adição n-terminal à proteína amilase madura
US7618801B2 (en) 2007-10-30 2009-11-17 Danison US Inc. Streptomyces protease
KR101683302B1 (ko) 2008-03-28 2016-12-06 다니스코 유에스 인크. 박테리아 세포 내 로커스 증폭 방법
JPWO2010082619A1 (ja) 2009-01-16 2012-07-05 大塚化学株式会社 高発現プロモーターおよびこれを用いた遺伝子産物製造法
WO2010097436A1 (fr) 2009-02-27 2010-09-02 Novozymes A/S Cellules mutantes à expression réduite de la métallopeptidase, appropriées pour la production de polypeptides recombinés
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
WO2011000924A1 (fr) 2009-07-03 2011-01-06 Abbott Products Gmbh Amylase séchée par pulvérisation, préparations pharmaceutiques la comprenant et son utilisation
CN102762723A (zh) 2009-12-21 2012-10-31 诺维信股份有限公司 用于在巯基-二硫键氧还酶缺陷的细菌突变体细胞中产生异源多肽的方法
CN103443278B (zh) 2011-03-23 2017-07-04 诺维信公司 生产分泌型多肽的方法
CN106754833B (zh) * 2017-01-16 2020-06-09 广东溢多利生物科技股份有限公司 在枯草芽孢杆菌中高效表达普鲁兰酶的方法及重组枯草芽孢杆菌
US20200318086A1 (en) 2017-11-10 2020-10-08 Novozymes A/S Temperature-sensitive cas9 protein
US20210017544A1 (en) 2017-12-22 2021-01-21 Novozymes A/S Counter-Selection by Inhibition of Conditionally Essential Genes
EP3927837A1 (fr) 2019-02-20 2021-12-29 Basf Se Procédé de fermentation industrielle pour bacillus utilisant un milieu défini et une alimentation en magnésium
MX2021010109A (es) 2019-02-20 2021-09-21 Basf Se Proceso de fermentacion industrial para bacillus mediante el uso de un medio definido y una alimentacion de oligoelementos.
EP3969576A1 (fr) 2019-05-15 2022-03-23 Novozymes A/S Endonucléase guidée par arn sensible à la température
CN114207125B (zh) 2019-06-25 2025-01-28 诺维信公司 通过抑制条件性必需基因进行反向选择
WO2022018260A1 (fr) 2020-07-24 2022-01-27 Basf Se Délétion unique et transcomplémentation de l'alanine racémase
EP4189103A1 (fr) 2020-07-28 2023-06-07 Basf Se Procédé de fermentation industrielle pour bacillus utilisant un décalage de température
MX2023001273A (es) 2020-07-28 2023-03-03 Basf Se Proceso de fermentacion industrial para bacillus mediante el uso de cambio de velocidad de alimentacion.
BR112023001392A2 (pt) 2020-07-28 2023-02-14 Basf Se Método para produzir uma proteína de interesse a partir de uma célula hospedeira de bacillus
US20230331775A1 (en) 2020-09-22 2023-10-19 Basf Se Method for recovering a protein from a fermentation broth comprising a high degree of lysed cells
EP4225779A2 (fr) 2020-10-07 2023-08-16 Basf Se Cellule de bacillus présentant des activités annexes réduites de lipase et/ou d'estérase
EP4263822A1 (fr) 2020-12-15 2023-10-25 Novozymes A/S Cellules hôtes mutées à motilité cellulaire réduite
MX2023015460A (es) 2021-06-24 2024-01-18 Basf Se Celula huesped de bacillus mejorada con proteina rema/remb alterada.
WO2022269082A1 (fr) 2021-06-24 2022-12-29 Basf Se Hôte de production de bacillus amélioré
EP4359423A1 (fr) 2021-06-24 2024-05-01 Basf Se Cellule hôte de bacillus licheniformis pour la production d'un composé d'intérêt avec une pureté accrue
US20250051822A1 (en) 2021-12-20 2025-02-13 Basf Se Method for improved production of intracellular proteins in bacillus
CN119630806A (zh) 2022-08-02 2025-03-14 巴斯夫欧洲公司 使用芽孢杆菌属宿主细胞与盐进料的稳定的蛋白质生产方法
CN120500529A (zh) 2023-01-05 2025-08-15 巴斯夫欧洲公司 折叠酶用于改善分泌分子的异源表达的用途

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Also Published As

Publication number Publication date
CA2123496A1 (fr) 1993-05-27
CA2123496C (fr) 2004-02-03
DK0667910T3 (da) 2003-12-01
EP0667910A1 (fr) 1995-08-23
FI942228L (fi) 1994-07-13
WO1993010249A1 (fr) 1993-05-27
FI942228A0 (fi) 1994-05-13
AU661070B2 (en) 1995-07-13
IL103750A0 (en) 1993-04-04
AU2943092A (en) 1993-06-15
JP3529774B2 (ja) 2004-05-24
US5698415A (en) 1997-12-16
DE69233178D1 (de) 2003-10-02
ATE248223T1 (de) 2003-09-15
DE69233178T2 (de) 2004-06-17
EP0667910B1 (fr) 2003-08-27
JPH07504085A (ja) 1995-05-11

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