HK40048819B

HK40048819B - Anti-microbial coating formulation

Info

Publication number: HK40048819B
Application number: HK42021038728.8A
Authority: HK
Inventors: Daniel Moros; Craig GROSSMAN; Gavri GROSSMANN
Original assignee: Allied Bioscience, Inc
Priority date: 2014-11-04
Filing date: 2021-09-10
Publication date: 2023-04-28

Description

Field Of The Invention

Embodiments generally relate to a chemical composition and a method using that composition to form a self decontaminating surface. In certain embodiments, the chemical composition comprises a photocatalyst. In certain embodiments, the photocatalyst comprises a titanium oxide moiety. In certain embodiments, the chemical composition comprises an organosilane.

Background Of The Invention

In a publication entitled "Evaluation of Two Organosilane Products for Sustained Antimicrobial Activity on High-Touch Surfaces in Patient Rooms, American Journal of Infection Control 42 (2014) 326-8, reports, inter alia, "[t]o the best of our knowledge, ours is the first published controlled trial of applying organosilane compounds to high-touch surfaces in patient rooms as a strategy for reducing the level of microbial contamination of environmental surfaces between daily cleanings." Id. at 327.

The authors found the two organosilanes ineffective for any sort of sustained antimicrobial efficacy. "In conclusion, our study was not able to demonstrate sustained antimicrobial activity for either organosilane product tested when applied to high-touch surfaces." Id. at 328.

Brief Description Of The Drawings

The invention will be better understood from a reading of the following detailed description taken in conjunction with the drawings in which like reference designators are used to designate like elements, and in which:

FIG. 1 graphically shows the number of hospital acquired C-difficile infections in the Glendale Memorial Hospital ICU from January 2012 through February 2014;
FIG. 2 graphically shows the number of hospital acquired C- difficile infections at the Glendale Memorial Hospital (excluding ICU) from January 2012 through February 2014; and
FIG. 3 illustrates Applicants' sterilizing station 300.

Detailed Description Of Preferred Embodiments

The invention is described in preferred embodiments in the following description with reference to the Figures, in which like numbers represent the same or similar elements. Reference throughout this specification to "one embodiment," "an embodiment," or similar language means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment of the present invention. Thus, appearances of the phrases "in one embodiment," "in an embodiment," and similar language throughout this specification may, but do not necessarily, all refer to the same embodiment.

The described features, structures, or characteristics of the invention may be combined in any suitable manner in one or more embodiments. In the following description, numerous specific details are recited to provide a thorough understanding of embodiments of the invention. One skilled in the relevant art will recognize, however, that the invention may be practised without one or more of the specific details, or with other methods, components, materials, and so forth. In other instances, well-known structures, materials, or operations are not shown or described in detail to avoid obscuring aspects of the invention.

In certain embodiments of Applicants' composition and method, a coating is formed on a surface of an object, where that coating comprises a plurality of titanium - oxygen bonds, where that coating is formed by disposing on the surface a mixture of Peroxotitanium acid solution and Peroxo-modified anatase sol (collectively "Titanium-Oxygen Moieties").

In certain embodiments, Applicants' Titanium-Oxygen Moieties comprise up to about a total of one weight percent loading of the mixture of Peroxotitanium acid solution and Peroxo-modified anatase sol. In certain embodiments, Applicants' Titanium-Oxygen Moieties comprise about 0.5 weight percent Peroxotitanium acid solution in combination with about 0.5 weight percent Peroxo-modified anatase sol.

A method to prepare both Peroxotitanium acid solution and Peroxo-modified anatase sol is disclosed in Journal of Sol-Gel Science and In yet other embodiments, R2 is alkyl with an chlorine moiety. In still other embodiments, R2 is alkyl with a quarternary ammonium group.

In certain embodiments, after application of Applicants' silanetriol 2 to either a hard surface, i.e. wall, door, table, or a soft surface, i.e. bedding, draperies, furniture cushions, a resulting coating disposed on the hard surface / soft surface comprises a plurality of silsesquioxane 3 structures. In certain embodiments, after application of Applicants' silanetriol 2 in combination with titanium dioxide to either a hard surface, i.e. wall, door, table, or a soft surface, i.e. bedding, draperies, furniture cushions, a resulting coating disposed on the hard surface / soft surface comprises a plurality of silsesquioxane structures 3 in combination with Applicants' Titanium-Oxygen Moieties.

The following Examples are presented to further illustrate to persons skilled in the art how to make and use the invention. These Examples are not intended as limitations, however, upon the scope of the invention.

EXAMPLE I

A study was conducted at the Glendale Memorial Hospital and Health Center in Glendale, CA (the "Glendale Memorial Hospital Study"). The Center has a 24 bed intensive care (ICU). The study was performed between May 10 and September 30, 2013.

The Glendale Memorial Hospital Study was designed to assess the anti-microbial properties of Applicants' coating composition and method, wherein the method employed utilized an initial coating of Applicants' organosilane followed by an overspray of titanium dioxide. The entire ICU was subjected to the two step spray regime to treat all objects in each room including hard surfaces (beds, tray tables, bed rail, walls, etc.) and soft surfaces (drapes, cloth and vinyl covered chairs, woven fabrics, non-woven fabrics, leather goods). The goal of the Glendale Memorial Hospital Study was to assess the anti-microbial efficacy of Applicants' coating composition in a practical application in a health care environment.

Each surface was first electrostatically spray coated at room temperature using an aqueous composition formed by mixing Octadecylaminodimethyltrihydroxysilylpropyl Ammonium Chloride 6 at 3.6 weight percent in water.

About fifteen (15) minutes after the electrostatic spray coating using the aqueous mixture of Octadecylaminodimethyltrihydroxysilylpropyl Ammonium Chloride 6, most of the water had evaporated leaving a coating comprising at least ninety weight percent (90 wt %) Octadecylaminodimethyltrihydroxysilylpropyl Ammonium Chloride 6, Thereafter, each surface was electrostatically spray coated at room temperature using Applicants' Titanium-Oxide Moieties. After about 15 minutes, most of the water in the second spray deposition had evaporated leaving a coating comprising at least ninety weight percent (90 wt %) Applicants' Titanium-Oxide Moieties.

The treated surfaces were maintained at room temperature during the spray deposition of the aqueous Octadecylaminodimethyltrihydroxysilylpropyl Ammonium Chloride 6, and during the spray deposition of Applicants' Titanium-Oxide Moieties. None of the treated objects were subjected to any elevated heat treatment wherein the treated surface was heated to a temperature greater than about room temperature during or after completion of Applicants' spray coating regime.

Applicants have found that using their two step, spray coating protocol described hereinabove, after evaporation of the water from the spray deposited Titanium-Oxide Moieties and evaporation of the water portion from the spray deposited aqueous Octadecylaminodimethyltrihydroxysilylpropyl Ammonium Chloride, the combined weight of Applicants' Titanium-Oxide Moieties and Octadecylaminodimethyltrihydroxysilylpropyl Ammonium Chloride disposed on a treated surface was measured as 1,178 g/m² (0.76 mg/in²).

Initial microbial sampling of various fomites was conducted to assess the levels of bacteria on various hospital surfaces before selecting study sites. After review, 95 sites were selected for the study in the ICU. Each of the ninety-five (95) specific sites in the ICU were selected for recurring sampling at weeks 1, 2, 4, 8, and 15, after application of Applicants' composition. Those selected sites included bed rails, bed controls, tray tables, and walls above sinks. Samples were also collected from the two ICU nursing stations and waiting lobby including countertops, phones, computer keyboards, chair armrests and end tables. All movable items were inconspicuously tagged and coded over the course of the study so that the same objects could be sampled.

Each of the sites was cultured prior to application of Applicants' method and at 1 week (6-8 days), 2 weeks (13-17 days), 4 weeks (29-32 days), 8 weeks (59-62 days), 15 weeks (104-107 days) after application. Some objects were removed and were not available for culture at some of the subsequent time points. period May 2013 through November 2013. Thus, FIG. 1 shows that there was a single hospital acquired C-difficile infection originating in the ICU during the six month period May 2013 through November 2013.

FIG. 1 further shows that, other than the six month period May 2013 through November 2013, there was no other 6 month period during the 25 months from January 2012 through February 2014 wherein only a single hospital acquired C-difficile infection originated in the ICU. All surfaces in the ICU were treated as described hereinabove during the first week of May 2013 as part of the Glendale Memorial Hospital Study.

FIG. 2 graphically shows the number of hospital acquired C-difficile infections at the Glendale Memorial Hospital (excluding ICU) from January 2012 through February 2014. FIG. 2 indicates that, with the exception of April 2013, there were between 1 and 8 hospital acquired C-difficile infections every month during the 25 month period in hospital areas outside of the ICU. During the period May 2013 through November 2013, FIG. 2 shows that there were a total of 20 hospital acquired C-difficile infections originating outside of the ICU at the Glendale Memorial Hospital.

FIGs. 1 and 2 show that during the period May 2013 through November 2013, a single hospital acquired C-difficile infection originated in the ICU at the Glendale Memorial Hospital, and a total of 20 hospital acquired C-difficile infections originated outside of the ICU at the Glendale Memorial Hospital.

Applicants have found that they can dispose Octadecylaminodimethyltrihydroxysilylpropyl Ammonium Chloride and Applicants Titanium-Oxide Moieties, by spray deposition or by dip coating, onto a dressing prior to use of that dressing to cover a wound. As those skilled in the art will appreciate, a dressing is a sterile pad or compress applied to a wound to promote healing and/or prevent further harm. A dressing is designed to be in direct contact with the wound, as distinguished from a bandage, which is most often used to hold a dressing in place. In certain embodiments, Applicants' wound dressings including the following: alginates and other fiber gelling dressings including ropes and sheets, composite dressings, foam dressings with and without adhesive border, gauze with and without adhesive

Clean the test coupons with surface cleaner using a microfiber cloth.

Hold sprayer about eight (8) inches from surface to be cleaned.

Spray on let stand for 1-3 minutes and wipe it off, if the area is extremely dirty allow cleaner to stand longer, or apply a second spray and wipe.

Wipe surface with a clean, damp sponge or cloth.

Allow surface to completely dry.

With gloved hands examine coupons for consistency.

First Coat - Applicants' Titanium-Oxide Moieties application.

Add Applicants' Titanium-Oxide Moieties to the applicator container.

Fasten the Liquid Hose/Bottle cap assembly tightly on the container.

Connect the air hose from compressor to air fitting on the spray applicator.

Connect the liquid hose to the liquid fitting on the spray applicator.

Plug the power cord into an appropriate receptacle. Turn on the air compressor.

Optimal spraying distance is at least 91.4 to 121.9 cm (36 to 48 inches) away from the target surface.

Hold the spray gun at right angles to the target surface and spray.

Target surface should just barely glisten with the spray. Do not over-saturate the surface.

Rinse spray gun with distilled water prior to applying Applicants' Titanium-Oxide Moieties (unless using 2 sprayers, one for each product).

Second Coat - Organosilane application

Add the Octadecylaminodimethyltrihydroxysilylpropyl Ammonium Chloride 6 to applicator container.

Fasten the Liquid Hose/Bottle cap assembly tightly on the container.

Connect the air hose from compressor to air fitting on the spray applicator.

Connect the liquid hose to the liquid fitting on the spray applicator.

Plug the power cord into an appropriate receptacle. Turn on the air compressor.

Hold the spray gun at right angles to the target surface and spray.

Allow surface to completely dry.

Clean the spray gun with distilled water per manufactures' specifications after each day of use.

TABLE 9


		0 Hour	2 Hour	Log Reduction 2 Hours	6 Hour	Log Reduction 6 Hours
REVERSED ORDER OF COATING		1,000,000	41,000	1.39	140	3.85
Control	1,000,000	800,000	0.10	37,000	1.43

inoculation with MS-2. TABLE 14 recites efficacy data for the treated coupons after inoculation with MRSA.

In summary, TABLES 12, 13, and 14, demonstrates that simultaneously disposing Applicants' Titanium-Oxygen Moieties and Applicants' organosilane onto a target surface generates a self-decontaminating surface.

Procedure

Put on sterile gloves.

Prepare the test coupons by wiping them first with ISP Alcohol and allowing to dry.

Clean the test coupons with surface cleaner using a microfiber

Hold sprayer about 20.3 cm (eight (8) inches) from surface to be cleaned.

Wipe surface with a clean, damp sponge or cloth.

Allow surface to completely dry.

With gloved hands examine coupons for consistency. Prepare Combined Solution

In a measured container combine 50%

Octadecylaminodimethyltrihydroxysilylpropyl Ammonium Chloride aqueous mixture and 50% Applicants' Titanium-Oxide Moieties aqueous mixture.

Mix thoroughly.

Coating

Add the mixture from [00092] to applicator container.

Fasten the Liquid Hose/Bottle cap assembly tightly on the container.

Connect the air hose from compressor to air fitting on the spray applicator.

Connect the liquid hose to the liquid fitting on the spray applicator

Plug the power cord into an appropriate receptacle. Turn on the air compressor.

Hold the spray gun at right angles to the target surface and spray.

Allow surface to completely dry.

TABLE 12


		0 Hour	2 Hour	Log Reduction 2 Hours	6 Hour	Log Reduction 6 Hours
SIMULTANEOUS APPLICATION	1,000,000	42,000	1.38	110	3.96
Control	1,000,000	800,000	0.10	37,000	1.43

TABLE 13


		0 Hour	2 Hour	Log Reduction 2 Hours
SIMULTANEOUS APPLICATION	1,000,000	42,000	2.70
Control	21,000,000	110,000	2.28

TABLE 14


		0 Hour	2 Hour	Log Reduction 2 Hours	6 Hour	Log Reduction 6 Hours	24 Hour	Log Reduction 24 Hours
SIMULTANEOUS APPLICATION	3,700,000	130,000	1,45	7,000	2.72	1,400	3,42
Control	3,700,000	2,300,000	0.21	58,000	1.80	5,800	2.80

EXAMPLE IV

Example IV utilizes (3-Aminopropyl)triethoxysilane in water as the only organosilane. This being the case, this Example VI utilizes NO organosilane(s) comprising a quaternary ammonium moiety. (3-Aminopropyl)triethoxysilane is rapidly hydrolyzed to (3- Aminopropyl)trihydroxysilane) 7 when mixed with water.

The test coupons of this Example IV were prepared and using the Procedure recited immediately hereinbelow. In certain embodiments, the treated coupons were stored for at least four (4) weeks prior to inoculation with various organisms.

Applicants have found that using their two step, spray coating protocol described hereinbelow, after evaporation of the water from the spray deposited Titanium-Oxide Moieties and evaporation of the water portion from the spray deposited aqueous (3-Aminopropyl)trihydroxysilane), the combined weight of

Applicants' Titanium-Oxide Moieties and (3-Aminopropyl)trihydroxysilane) disposed on a treated surface was measured as 1.22 mg/in².

Applicants have found that they can dispose (3-Aminopropyl)trihydroxysilane and Applicants' Titanium-Oxide Moieties, by spray deposition or by dip coating, onto a dressing prior to use of that dressing to cover a wound. As those skilled in the art will appreciate, a dressing is a sterile pad or compress applied to a wound to promote healing and/or prevent further harm. A dressing is designed to be in direct contact with the wound, as distinguished from a bandage, which is most often used to hold a dressing in place. In certain embodiments, Applicants' wound dressings including the following: alginates and other fiber gelling dressings including ropes and sheets, composite dressings, foam dressings with and without adhesive border, gauze with and without adhesive border,hydrocolloids, specialty absorptive dressings with and without adhesive borders, transparent films, collagen dressings sheets and ropes, hydrogel sheets with and without adhesive border, cotton packing strips, roll gauze, paper tape, silk tape, compression bandages (elastic, knitted/woven), self-adherent bandage (elastic, non-knitted/non-woven).

TABLES 15, 16, and 17, recite efficacy data for the treated coupons after inoculation with E. coli. In summary, TABLES 15, 16, and 17, demonstrate that disposing a 3-Aminopropyl)trihydroxysilane coating onto a target surface, and then disposing TiO₂ over that 3-Aminopropyl)trihydroxysilane coating generates a self-decontaminating surface.

Procedure

Put on sterile gloves.

Clean the test coupons with surface cleaner using a microfiber cloth.

Hold sprayer about eight (8) inches from surface to be cleaned.

Wipe surface with a clean, damp sponge or cloth.

Allow surface to completely dry.

With gloved hands examine coupons for consistency.

Prepare Dilution for (3-Aminopropyl)triethoxysilane

Prepare a 10% solution of 3-Aminopropyl)triethoxysilane in Methanol (MeOH) (10 ml silane in 100 ml MeOH).

Prepare Triethanolamine as a 10% solution in MeOH.

Combine the triethanolamine solution and 3-Aminopropyl)triethoxysilane solution in a 1:1 ratio on a stir plate at room temperature (ie-100 ml triethanolamine solution added to 100 ml 3-Aminopropyl)triethoxysilane solution.

First Coat - (3-Aminopropyl)triethoxysilane application

Add the mixture from [000116] to the applicator container.

Fasten the Liquid Hose/Bottle cap assembly tightly on the container.

Connect the air hose from compressor to air fitting on the spray applicator.

Connect the liquid hose to the liquid fitting on the spray applicator.

Plug the power cord into an appropriate receptacle. Turn on the air compressor.

Hold the spray gun at right angles to the target surface and spray.

Rinse spray gun with distilled water prior to applying Applicants' Titanium Oxide Moieties (unless using 2 sprayers, one for each product).

Second Coat - Applicants' Titanium Oxide Moieties application.

Add Applicants' Titanium Oxide Moieties to the applicator container.

Fasten the Liquid Hose/Bottle cap assembly tightly on the container.

Connect the air hose from compressor to air fitting on the spray applicator.

Connect the liquid hose to the liquid fitting on the spray applicator.

Plug the power cord into an appropriate receptacle. Turn on the air compressor.

Hold the spray gun at right angles to the target surface and spray.

Allow surface to completely dry.

TABLE 15


Time Zero	Control	9.80E+06	9.21E+06	N.A.
	Control	8.65E+06	9.21E+06	N.A.
	(3-Aminopropyl) triethoxysilane	8.20E+06	8.05E+06
	(3-Aminopropyl) triethoxysilane	7.90E+06	8.05E+06

EXAMPLE V

Example V mixes (3-Chloropropyl)trimethoxysilane in water. (3-Chloropropyl)trimethoxysilane is immediately hydrolyzed to (3-Chloropropyl)trihydroxysilane 8 when mixed with water.

This being the case, this Example V utilizes NO organosilane(s) comprising a quaternary ammonium moiety. Furthermore, this Example VII utilizes NO organosilane(s) comprising an amino moiety.

The test coupons of this Example V were prepared using the Procedure recited immediately hereinbelow. In certain embodiments, the treated coupons were stored for at least four (4) weeks prior to inoculation with various organisms.

Applicants have found that they can dispose (3-Chloropropyl)trihydroxysilane and Applicants' Titanium-Oxide Moieties, by spray deposition or by dip coating, onto a dressing prior to use of that dressing to cover a wound. As those skilled in the art will appreciate, a dressing is a sterile pad or compress applied to a wound to promote healing and/or prevent further harm. A dressing is designed to be in direct contact with the wound, as distinguished from a bandage, which is most often used to hold a dressing in place. In certain embodiments, Applicants' wound dressings including the following: alginates and other fiber gelling dressings including ropes and sheets, composite dressings, foam dressings with and without adhesive border, gauze with and without adhesive border, hydrocolloids, specialty absorptive dressings with and without adhesive borders, transparent films, collagen dressings sheets and ropes, hydrogel sheets with and without adhesive border, cotton packing strips, roll gauze, paper tape, silk tape, compression bandages (elastic, knitted/woven), self-adherent bandage (elastic, non-knitted/non-woven).

TABLES 18, 19, and 20, recite efficacy data for the treated coupons after inoculation with E. coli. In summary, TABLES 18, 19, and 20, demonstrates that disposing a (3-Chloropropyl)trihydroxysilane coating on a target surface followed by disposing Applicants' Titanium Oxide Moieties onto the (3- Chloropropyl)trihydroxysilane coating generates a self-decontaminating surface.

Procedure

Put on sterile gloves.

Clean the test coupons with surface cleaner using a microfiber cloth.

Hold sprayer about 20.3 cm (eight (8) inches) from surface to be cleaned.

Wipe surface with a clean, damp sponge or cloth.

Allow surface to completely dry.

With gloved hands examine coupons for consistency.

Prepare Organosilane Dilution for (3-Chloropropyl)trimethoxy silane

Prepare a 10% solution of (3-Chloropropyl)trimethoxy silane in Methanol (MeOH) (10 ml. silane in 100 ml. MeOH).

Prepare Triethanolamine solution as a 10% solution in MeOH.

Combine the triethanolamine solution and (3- Chloropropyl)trimethoxy silane solution in a 1:1 ratio on a stir plate at room temperature (ie-100 ml. trethanolamine added to 100 ml. (3-Chloropropyl)trimethoxy silane).

First Coat - (3-Chloropropyl)trimethoxy silane application [000156]

Add the mixture of [000149] to the applicator container. [000157] Fasten the Liquid Hose/Bottle cap assembly tightly on the container.

Connect the air hose from compressor to air fitting on the spray applicator.

Connect the liquid hose to the liquid fitting on the spray applicator.

Plug the power cord into an appropriate receptacle. Turn on the air compressor.

Hold the spray gun at right angles to the target surface and spray.

Second Coat - Applicants' Titanium Oxide Moieties application.

Add Applicants' Titanium Oxide Moieties to the applicator container.

Fasten the Liquid Hose/Bottle cap assembly tightly on the container.

Connect the air hose from compressor to air fitting on the spray applicator.

Connect the liquid hose to the liquid fitting on the spray applicator.

Plug the power cord into an appropriate receptacle. Turn on the air compressor.

Hold the spray gun at right angles to the target surface and spray.

Allow surface to completely dry.

TABLE 18


Time Zero	Control	9.80E+06	9.21E+06	N.A.
	Control	8.65E+06	9.21E+06	N.A.

		1.16E+07	1.00E+07	-0.04	-8.9%
	3-Chloropropyl)	8.70E+06	1.00E+07	-0.04	-8.9%

TABLE 19


1 Hour	Control	3.35E+06	3.61E+06	N.A.
	Control	3.90E+06	3.61E+06	N.A.

	(3-Chloropropyl) trimethoxysilane	1.10E+03	2.35E+02	4.19	0 99.994%
	(3-Chloropropyl) trimethoxysilane	5.00E+01	2.35E+02	4.19	0 99.994%

TABLE 19

a "≤": No bacterial colonies observed, therefore counts at or below limit of detection (based on 0.1 ml plating volume)

TABLE 20


4 Hours	Control	2.80E+05	3.91 E+05	N.A.
	Control	5.45E+05	3.91 E+05	N.A.

	(3-Chloropropyl) trimethoxysilane	≤ 5.00E+01	≤ 5.00E+01	≥ 3.89	≥99 987
	(3-Chloropropyl) trimethoxysilane	≤ 5.00E+01	≤ 5.00E+01	≥ 3.89	≥99 987

TABLE 20

EXAMPLE VI

This Example VI utilizes three (3) coating formulations without any Titanium-Oxide containing compounds. A first of the three coating formulations identified in this Example VI as ABS 2015E utilizes Octadecylaminodimethyltrihydroxysilylpropyl Ammonium Chloride 6 as the organosilane. A second of the three coating formulations identified in this Example VI as ABS 2020E utilizes (3-Aminopropyl)trihydroxysilane) 7 as the organosilane. A third of the three coating formulations identified in this Example VI as ABS 2030E utilizes (3-Chloropropyl)trihydroxysilane) 8 as the organosilane.

The method of Example IV from Paragraph [000105] through Paragraph [000124] relating to spray deposition of a silane onto test coupons was utilized in this Example VI. The method of Paragraph [000125] through and including Paragraph [000134] relating to spray deposition of the Titanium-Oxygen Moieties was not utilized in this Example VI.

Carriers were still not completely dry even after 24 hours drying time. Twenty (20) microliters of 0/N cultures of E.coli 25592 (grown at 37°C for 18 hours) were added to each carrier. Following inoculation of the carriers, the carriers were swabbed with D/E neutralizing broth and processed for the zero hour time point. This was repeated for the land 4 hour time points.

The collected samples were then diluted in PBS and 100 microliters were plated on to TSA plates and left overnight at 37 °C before counting and calculating cfu/ml. All carriers were tested in duplicate and two experiments were performed (A and B in data tables) in tandem. All data is represented as the mean+/- the SEM (standard error) when applicable.

When calculated relative to the timed control, choline bitartrate showed the greatest surface-kill, with a 2.39 log reduction in bacteria. Acetylcholine chloride and choline chloride showed a 1.85 and 1.40 log reduction, respectively. When compared with the results of aqueous solutions of Aminopropyl triethoxysilane (APTES) and the cholines at the same concentration, it is clear that these solutions are much more antimicrobial than the cholines on their own. APTES +Choline Chloride and APTES +Choline Bitartrate showed a 3.36 and 3.38 log reduction, respectively, at the 1 hour time point.

TABLE 24 recites antimicrobial efficacy data for the above-described choline formulations at time To, i.e. immediately after inoculation. TABLE 25 recites antimicrobial data at one (1) hour after inoculation. fewer than seven (7) UV emitters per side. In still other embodiments, Applicants' sterilizing station comprises more than seven (7) UV emitters per side.

Side 320 is similarly formed to include a plurality of UV emitters, where each of those UV emitters face the interior, i.e. walking space portion, of sterilizing station 300. The plurality of UV emitters disposed on the interior portion of side 310 have a facing relationship with the plurality of UV emitters disposed on the interior portion of side 320.

Further in the illustrated embodiment of FIG. 3, top portion 330 comprises a plurality of UV emitters, i.e. UV emitters 332 and 334, where those UV emitters face downwardly. In other embodiments, top portion 330 comprises more than two (2) UV emitters.

The illustrated embodiment of FIG. 3 shows a medical practitioner walking through sterilizing station 300. The medical practitioner is wearing a scrub suit, the various pieces of which have been coated on the exterior surface with Applicants' coating composition. As the practitioner walks through sterilizing station 300, the plurality of UV emitters disposed on sides 310 and 320, and the plurality of UV emitters disposed on top 330, are energized thereby maximizing the photocatalytic effect of Applicants' coating. Enhancing the photocatalytic activity of the coating maximizes the production of high energy, atomic oxygen species at the surface of scrub suit pieces, thereby, effectively sterilizing the exterior surfaces of all scrub suit articles.

While the preferred embodiments of the present invention have been illustrated in detail, it should be apparent that modifications and adaptations to those embodiments may occur to one skilled in the art without departing from the scope of the present invention as set forth herein.

Claims

An anti-microbial coating formulation consisting essentially of triethanolamine and a silane having a structure:
The anti-microbial coating formulation according to claim 1, wherein the triethanolamine is present in the formulation at 5% (v/v).
The anti-microbial coating formulation according to claims 1 or 2, wherein the silane is present in the formulation at 5% (v/v).
The anti-microbial coating formulation according to any of the preceding claims, wherein the triethanolamine is present in the formulation at 5% (v/v), wherein the silane is present in the formulation at 5% (v/v), the remainder of the formulation being methanol.
The anti-microbial coating formulation according to claim 1 for use in preparing a coating on a stainless steel surface that exhibits a 4.74 log reduction in murine norovirus 1 hour after initial inoculation of the coating with murine norovirus.
The anti-microbial coating formulation according to claim 1 for use in preparing a coating on a stainless steel surface that exhibits a 5.55 log reduction in murine norovirus 4 hours after initial inoculation of the coating with murine norovirus.
The anti-microbial coating formulation according to claim 5 or 6, wherein the use comprises spray coating the anti-microbial coating formulation on the stainless steel surface.
Use of the anti-microbial coating formulation according to claims 1-4 against murine norovirus on a surface.
The use of claim 8, wherein the surface is stainless steel surface.