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IN2014MN01628A - - Google Patents

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Publication number
IN2014MN01628A
IN2014MN01628A IN1628MUN2014A IN2014MN01628A IN 2014MN01628 A IN2014MN01628 A IN 2014MN01628A IN 1628MUN2014 A IN1628MUN2014 A IN 1628MUN2014A IN 2014MN01628 A IN2014MN01628 A IN 2014MN01628A
Authority
IN
India
Prior art keywords
nucleic acid
acid amplification
amplification reaction
microchip
reaction
Prior art date
Application number
Inventor
Masahiro Matsumoto
Masaki Sato
Hidetoshi Watanabe
Original Assignee
Sony Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sony Corp filed Critical Sony Corp
Publication of IN2014MN01628A publication Critical patent/IN2014MN01628A/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5088—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above confining liquids at a location by surface tension, e.g. virtual wells on plates, wires
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/069—Absorbents; Gels to retain a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Biomedical Technology (AREA)
  • Dispersion Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

Provided is a method for producing a microchip for use in a nucleic acid amplification reaction which enables a simple and highly accurate analysis. Provided is a method for producing a microchip for use in a nucleic acid amplification reaction comprising: a solidification step of drying a reagent solution containing at least a portion of a substance needed for the nucleic acid amplification reaction; and an accommodation step of placing a reagent containing the solidified substance in a well which is a reaction place for the nucleic acid amplification reaction. In the microchip for use in a nucleic acid amplification reaction which is produced by the production method the substance needed for the nucleic acid amplification reaction is accommodated in a solidified state. Therefore it becomes possible to inhibit the occurrence of a non specific amplification in a nucleic acid amplification reaction and therefore it becomes possible to carry out an analysis with high accuracy.
IN1628MUN2014 2012-03-08 2013-01-16 IN2014MN01628A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2012052322 2012-03-08
PCT/JP2013/050652 WO2013132891A1 (en) 2012-03-08 2013-01-16 Method for producing microchip for use in nucleic acid amplification reaction

Publications (1)

Publication Number Publication Date
IN2014MN01628A true IN2014MN01628A (en) 2015-05-15

Family

ID=49116378

Family Applications (1)

Application Number Title Priority Date Filing Date
IN1628MUN2014 IN2014MN01628A (en) 2012-03-08 2013-01-16

Country Status (8)

Country Link
US (1) US9545630B2 (en)
EP (1) EP2824172B1 (en)
JP (1) JP5987895B2 (en)
KR (1) KR20140143139A (en)
CN (1) CN104160011A (en)
IN (1) IN2014MN01628A (en)
RU (1) RU2014135538A (en)
WO (1) WO2013132891A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10775401B2 (en) 2016-03-15 2020-09-15 Abbott Molecular Inc. Systems and methods for automated analysis
WO2018109829A1 (en) * 2016-12-13 2018-06-21 栄研化学株式会社 Microchip
CN110885902B (en) * 2018-09-10 2022-10-21 北京亿森宝生物科技有限公司 Freeze-drying microchip, kit and method for detecting porcine reproductive and respiratory syndrome virus and identifying highly pathogenic classical variant strain of porcine reproductive and respiratory syndrome virus
CN110885899B (en) * 2018-09-10 2023-04-14 中国动物疫病预防控制中心（农业部屠宰技术中心） Freeze-drying microchip, kit and method for identifying 16 avian disease pathogens
WO2025094815A1 (en) * 2023-10-30 2025-05-08 Ｔｏｐｐａｎホールディングス株式会社 Fluid device

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0775518B2 (en) * 1989-09-29 1995-08-16 勝 大鶴 Mushroom protein for eating and drinking, which is effective in preventing and treating hypertension, hyperlipidemia and obesity, and its extraction method
US5556771A (en) * 1995-02-10 1996-09-17 Gen-Probe Incorporated Stabilized compositions of reverse transcriptase and RNA polymerase for nucleic acid amplification
JPH08291078A (en) * 1995-04-21 1996-11-05 M I O:Kk Mushroom protein for food and beverage effective in preventing and treating hypertension and hyperlipemia and having antitumor action, mushroom protein for food and beverage effective in preventing and treating obesity and having antitumor action and method for extracting these proteins
JPH10234822A (en) * 1997-02-28 1998-09-08 Material Eng Tech Lab Inc Container for lyophilization
JP3626986B2 (en) * 1999-05-19 2005-03-09 有限会社つくば食料科学研究所 Freeze-drying method, apparatus and freeze-dried product
US6875619B2 (en) * 1999-11-12 2005-04-05 Motorola, Inc. Microfluidic devices comprising biochannels
ES2180416B1 (en) * 2001-03-12 2004-06-01 BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. PROCEDURE FOR THE PREPARATION OF STABILIZED REACTION MIXTURES, TOTAL OR PARTIALLY DESIRED, THAT INCLUDE, AT LEAST, ONE ENZYME, REACTION MIXES AND KITS CONTAINING THEM.
GB0414815D0 (en) * 2004-07-02 2004-08-04 Secr Defence Method for stabilising reagents which are useful for nucleic acid amplification
CN100460013C (en) 2006-09-05 2009-02-11 重庆康卫生物科技有限公司 Oral recombinant Helicobacter pylori vaccine and its preparation method
EP2344679B1 (en) 2008-09-24 2018-05-23 First Light Biosciences, Inc. Kits and devices for detecting analytes
SG171899A1 (en) * 2008-12-25 2011-07-28 Universal Bio Research Co Ltd Method for pretreating specimen and method for assaying biological substance
US8368882B2 (en) * 2009-01-30 2013-02-05 Gen-Probe Incorporated Systems and methods for detecting a signal and applying thermal energy to a signal transmission element
JP5691187B2 (en) * 2010-02-10 2015-04-01 ソニー株式会社 Microchip for nucleic acid amplification reaction and method for producing the same
JP5786295B2 (en) * 2010-06-22 2015-09-30 ソニー株式会社 Nucleic acid isothermal amplification microchip, method for producing the same, and nucleic acid isothermal amplification method
JP2012080870A (en) 2010-09-16 2012-04-26 Sony Corp Method for quantifying nucleic acid and microchip for nucleic acid amplification reaction

Also Published As

Publication number Publication date
JP5987895B2 (en) 2016-09-07
KR20140143139A (en) 2014-12-15
JPWO2013132891A1 (en) 2015-07-30
US20150017318A1 (en) 2015-01-15
EP2824172B1 (en) 2017-09-06
EP2824172A4 (en) 2015-11-04
WO2013132891A1 (en) 2013-09-12
CN104160011A (en) 2014-11-19
EP2824172A1 (en) 2015-01-14
RU2014135538A (en) 2016-03-20
US9545630B2 (en) 2017-01-17

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