JP2000508624A - コンビナトリアルライブラリーのスクリーニングの間にタンパク質の化学物質に対する親和性を測定する方法 - Google Patents

コンビナトリアルライブラリーのスクリーニングの間にタンパク質の化学物質に対する親和性を測定する方法

Info

Publication number: JP2000508624A
Authority: JP; Japan
Prior art keywords: protein; compound; target; affinity; gradient
Prior art date: 1996-03-08
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Pending

Application number

JP9531978A

Other languages

English (en)

Japanese (ja)

Inventor

ダニントン，ダミアン・ジェイ

ヤマシタ，デニス・シンジ

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

SmithKline Beecham Corp

Original Assignee

SmithKline Beecham Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1996-03-08

Filing date

1997-03-07

Publication date

2000-07-11

1997-03-07 Application filed by SmithKline Beecham Corp filed Critical SmithKline Beecham Corp

2000-07-11 Publication of JP2000508624A publication Critical patent/JP2000508624A/ja

Status Pending legal-status Critical Current

Links

102000004169 proteins and genes Human genes 0.000 title claims abstract description 37
108090000623 proteins and genes Proteins 0.000 title claims abstract description 37
238000000034 method Methods 0.000 title claims abstract description 22
239000000126 substance Substances 0.000 title claims abstract description 9
238000012216 screening Methods 0.000 title claims abstract description 7
150000001875 compounds Chemical class 0.000 claims abstract description 45
238000003384 imaging method Methods 0.000 claims abstract description 8
239000007787 solid Substances 0.000 claims abstract description 6
238000009826 distribution Methods 0.000 claims abstract description 4
238000012412 chemical coupling Methods 0.000 claims description 5
230000015572 biosynthetic process Effects 0.000 claims description 4
238000003786 synthesis reaction Methods 0.000 claims description 3
239000007790 solid phase Substances 0.000 claims description 2
238000005259 measurement Methods 0.000 claims 1
238000009828 non-uniform distribution Methods 0.000 claims 1
230000008878 coupling Effects 0.000 abstract description 11
238000010168 coupling process Methods 0.000 abstract description 11
238000005859 coupling reaction Methods 0.000 abstract description 11
238000004458 analytical method Methods 0.000 abstract description 2
235000018102 proteins Nutrition 0.000 description 29
239000002245 particle Substances 0.000 description 17
239000002202 Polyethylene glycol Substances 0.000 description 15
229920001223 polyethylene glycol Polymers 0.000 description 15
230000003287 optical effect Effects 0.000 description 10
229920000642 polymer Polymers 0.000 description 10
239000003153 chemical reaction reagent Substances 0.000 description 5
238000001125 extrusion Methods 0.000 description 4
239000012528 membrane Substances 0.000 description 4
239000004793 Polystyrene Substances 0.000 description 3
KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 description 3
230000000903 blocking effect Effects 0.000 description 3
125000005998 bromoethyl group Chemical group 0.000 description 3
239000012895 dilution Substances 0.000 description 3
238000010790 dilution Methods 0.000 description 3
IWDCLRJOBJJRNH-UHFFFAOYSA-N para-hydroxytoluene Natural products CC1=CC=C(O)C=C1 IWDCLRJOBJJRNH-UHFFFAOYSA-N 0.000 description 3
229920002223 polystyrene Polymers 0.000 description 3
239000011347 resin Substances 0.000 description 3
229920005989 resin Polymers 0.000 description 3
229910000104 sodium hydride Inorganic materials 0.000 description 3
239000012312 sodium hydride Substances 0.000 description 3
239000002904 solvent Substances 0.000 description 3
239000000758 substrate Substances 0.000 description 3
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
239000011324 bead Substances 0.000 description 2
238000006243 chemical reaction Methods 0.000 description 2
238000005520 cutting process Methods 0.000 description 2
238000011534 incubation Methods 0.000 description 2
239000003446 ligand Substances 0.000 description 2
BVJSUAQZOZWCKN-UHFFFAOYSA-N p-hydroxybenzyl alcohol Chemical compound OCC1=CC=C(O)C=C1 BVJSUAQZOZWCKN-UHFFFAOYSA-N 0.000 description 2
239000000243 solution Substances 0.000 description 2
JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
125000003143 4-hydroxybenzyl group Chemical group [H]C([*])([H])C1=C([H])C([H])=C(O[H])C([H])=C1[H] 0.000 description 1
108091003079 Bovine Serum Albumin Proteins 0.000 description 1
102000004190 Enzymes Human genes 0.000 description 1
108090000790 Enzymes Proteins 0.000 description 1
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
239000007995 HEPES buffer Substances 0.000 description 1
229920000361 Poly(styrene)-block-poly(ethylene glycol) Polymers 0.000 description 1
230000003213 activating effect Effects 0.000 description 1
239000012190 activator Substances 0.000 description 1
239000000556 agonist Substances 0.000 description 1
150000004703 alkoxides Chemical class 0.000 description 1
239000002168 alkylating agent Substances 0.000 description 1
229940100198 alkylating agent Drugs 0.000 description 1
230000003281 allosteric effect Effects 0.000 description 1
150000001412 amines Chemical class 0.000 description 1
125000003277 amino group Chemical group 0.000 description 1
239000005557 antagonist Substances 0.000 description 1
238000003491 array Methods 0.000 description 1
PASDCCFISLVPSO-UHFFFAOYSA-N benzoyl chloride Chemical compound ClC(=O)C1=CC=CC=C1 PASDCCFISLVPSO-UHFFFAOYSA-N 0.000 description 1
229940098773 bovine serum albumin Drugs 0.000 description 1
239000000872 buffer Substances 0.000 description 1
239000005018 casein Substances 0.000 description 1
BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
235000021240 caseins Nutrition 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
238000003776 cleavage reaction Methods 0.000 description 1
230000008021 deposition Effects 0.000 description 1
238000001514 detection method Methods 0.000 description 1
IJRJBQGVWNVZSA-UHFFFAOYSA-N dilC18(3)(1+) Chemical compound CC1(C)C2=CC=CC=C2N(CCCCCCCCCCCCCCCCCC)C1=CC=CC1=[N+](CCCCCCCCCCCCCCCCCC)C2=CC=CC=C2C1(C)C IJRJBQGVWNVZSA-UHFFFAOYSA-N 0.000 description 1
239000003814 drug Substances 0.000 description 1
239000000975 dye Substances 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
239000007850 fluorescent dye Substances 0.000 description 1
230000000415 inactivating effect Effects 0.000 description 1
150000002632 lipids Chemical class 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
239000003550 marker Substances 0.000 description 1
238000004949 mass spectrometry Methods 0.000 description 1
125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
-1 potassium methoxide Chemical class 0.000 description 1
BDAWXSQJJCIFIK-UHFFFAOYSA-N potassium methoxide Chemical compound [K+].[O-]C BDAWXSQJJCIFIK-UHFFFAOYSA-N 0.000 description 1
238000012545 processing Methods 0.000 description 1
238000011160 research Methods 0.000 description 1
230000000717 retained effect Effects 0.000 description 1
239000000523 sample Substances 0.000 description 1
230000007017 scission Effects 0.000 description 1
230000035945 sensitivity Effects 0.000 description 1
230000001235 sensitizing effect Effects 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
238000010532 solid phase synthesis reaction Methods 0.000 description 1
239000000725 suspension Substances 0.000 description 1
230000008961 swelling Effects 0.000 description 1
238000012360 testing method Methods 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
- C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00497—Features relating to the solid phase supports
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00497—Features relating to the solid phase supports
- B01J2219/00513—Essentially linear supports
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00277—Apparatus
- B01J2219/00497—Features relating to the solid phase supports
- B01J2219/00527—Sheets
- B01J2219/00536—Sheets in the shape of disks
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00725—Peptides
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/10—Libraries containing peptides or polypeptides, or derivatives thereof

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Immunology (AREA)
Molecular Biology (AREA)
Engineering & Computer Science (AREA)
Medicinal Chemistry (AREA)
Biomedical Technology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Hematology (AREA)
Analytical Chemistry (AREA)
Urology & Nephrology (AREA)
Physics & Mathematics (AREA)
Food Science & Technology (AREA)
Chemical Kinetics & Catalysis (AREA)
Microbiology (AREA)
Cell Biology (AREA)
Biotechnology (AREA)
General Physics & Mathematics (AREA)
Pathology (AREA)
Biophysics (AREA)
Genetics & Genomics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Investigating Or Analysing Biological Materials (AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

JP9531978A 1996-03-08 1997-03-07 コンビナトリアルライブラリーのスクリーニングの間にタンパク質の化学物質に対する親和性を測定する方法 Pending JP2000508624A (ja)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US1313696P	1996-03-08	1996-03-08
US60/013,136		1996-03-08
PCT/US1997/003625 WO1997033169A1 (fr)	1996-03-08	1997-03-07	Methode pour determiner l'affinite des proteines a l'egard des agents chimiques lors du criblage des bibliotheques combinatoires

Publications (1)

Publication Number	Publication Date
JP2000508624A true JP2000508624A (ja)	2000-07-11

Family

ID=21758497

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP9531978A Pending JP2000508624A (ja)	1996-03-08	1997-03-07	コンビナトリアルライブラリーのスクリーニングの間にタンパク質の化学物質に対する親和性を測定する方法

Country Status (3)

Country	Link
EP (1)	EP1025437A4 (fr)
JP (1)	JP2000508624A (fr)
WO (1)	WO1997033169A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US7183050B2 (en)	2001-04-18	2007-02-27	Krull Ulrich J	Gradient resolved information platform
US7291456B2 (en)	2002-01-24	2007-11-06	The Regents Of The University Of California	Method for determining differences in molecular interactions and for screening a combinatorial library
ATE421088T1 (de) *	2002-03-11	2009-01-15	Caprotec Bioanalytics Gmbh	Verbindungen und verfahren für die analyse des proteoms
US7635564B2 (en)	2002-10-25	2009-12-22	Agilent Technologies, Inc.	Biopolymeric arrays having replicate elements
AU2004206856B9 (en)	2003-01-16	2006-11-02	Caprotec Bioanalytics Gmbh	Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5143854A (en) *	1989-06-07	1992-09-01	Affymax Technologies N.V.	Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5591646A (en) *	1992-09-02	1997-01-07	Arris Pharmaceutical	Method and apparatus for peptide synthesis and screening
US5288514A (en) *	1992-09-14	1994-02-22	The Regents Of The University Of California	Solid phase and combinatorial synthesis of benzodiazepine compounds on a solid support
US5834318A (en) *	1995-05-10	1998-11-10	Bayer Corporation	Screening of combinatorial peptide libraries for selection of peptide ligand useful in affinity purification of target proteins

1997
- 1997-03-07 WO PCT/US1997/003625 patent/WO1997033169A1/fr not_active Application Discontinuation
- 1997-03-07 EP EP97908953A patent/EP1025437A4/fr not_active Withdrawn
- 1997-03-07 JP JP9531978A patent/JP2000508624A/ja active Pending

Also Published As

Publication number	Publication date
EP1025437A4 (fr)	2002-10-23
EP1025437A1 (fr)	2000-08-09
WO1997033169A1 (fr)	1997-09-12

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US6653151B2 (en)	2003-11-25	Dry deposition of materials for microarrays using matrix displacement
US7179638B2 (en)	2007-02-20	Microarrays and their manufacture by slicing
US6887701B2 (en)	2005-05-03	Microarrays and their manufacture
US20020015952A1 (en)	2002-02-07	Microarrays and their manufacture by slicing
JP4007459B2 (ja)	2007-11-14	高処理量検定法
CA2310684C (fr)	2011-05-10	Criblage a haut rendement en format continu
US5556748A (en)	1996-09-17	Methods of sandwich hybridization for the quantitative analysis of oligonucleotides
Mendes et al.	2015	Optimization of the magnetic recovery of hits from one-bead–one-compound library screens
US6541203B2 (en)	2003-04-01	Detecting structural or synthetic information about chemical compounds
Martens et al.	1999	A generic particle-based nonradioactive homogeneous multiplex method for high-throughput screening using microvolume fluorimetry
JP2000508624A (ja)	2000-07-11	コンビナトリアルライブラリーのスクリーニングの間にタンパク質の化学物質に対する親和性を測定する方法
WO2002083951A1 (fr)	2002-10-24	Dosage de proteine/ligand de fixation multiplexes avec marques a proteine pna
WO1997033169A9 (fr)	1997-11-13	Methode pour determiner l'affinite des proteines a l'egard des agents chimiques lors du criblage des bibliotheques combinatoires
WO2006113037A1 (fr)	2006-10-26	Méthode de photographie d'un ensemble de microsphères
EP0774116B1 (fr)	1999-11-03	Procede de criblage de banques de composes
WO2002010761A1 (fr)	2002-02-07	Microreseaux et leur fabrication par tranchage
Hoff et al.	2002	Lipoconjugates for the noncovalent generation of microarrays in biochemical and cellular assays
US20070172905A1 (en)	2007-07-26	Method for screening process conditions of a chemical coupling reaction and kits therefor
WO1997001098A1 (fr)	1997-01-09	Procede de detection, quantification et/ou identification d'un peptide
Anderson	0	Microarrays and their manufacture by slicing
Parandoosh et al.	1998	Synthesis coupled to scintillation proximity affinity screening
CA2405815A1 (fr)	2001-10-04	Procede de detection et/ou de quantification de premieres molecules
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JP2008233010A (ja)	2008-10-02	マイクロチャンバアレイを用いた蛍光偏光解消法による標的物質測定方法