JP2001108685A - Kit for detecting protein indoor dust - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a kit for detecting protein indoor dust for safely and simply detecting tick allergen and protein indoor dust which is food for ticks. SOLUTION: This kit for detecting protein indoor dust is composed of fiber aggregates and a compound which reacts with protein to form a color as essential components. The compound to react with protein to form a color is enveloped in a carrier isolated from the fiber aggregates. The compound to react with protein to form a color is ninhydrin.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a protein indoor dust detection kit for detecting the presence of protein indoor dust which causes reproduction of mite allergens and ticks.

[0002]

2. Description of the Related Art Household dust in general households includes inorganic substances such as sand, clay, glass, and rust, as well as human and animal hair, dandruff and other skin debris, pollen, mold, food waste, and carpet. And organic materials such as fibers derived from the same.

On the other hand, mites that live indoors and cause allergies are Dermatophagoides mite, mainly Dermatophagoides pteronyssinus and Dermatophagoides farinae. 3
A suitable temperature and humidity of 0 ° C. and a humidity of 60% or more, and abundant proteins such as human and animal hair, skin debris such as dandruff, pollen, mold, and food debris as feed.

[0004] Mites, mites carcasses, and excreta that live in the rooms of ordinary households contain components that induce allergic diseases such as asthma or eczema. For this reason, it is necessary to periodically remove the dust in the protein room, which is the bait of the mite that causes the mite allergen and the mite to propagate, by cleaning. However, it is difficult to judge how much cleaning should be done to remove mite allergens and mite bait protein indoor dust, and a method of detecting mite allergens and mite bait protein indoor dust is desired. Was rare.

[0005] As a method for detecting mite or mite allergen, there are methods using instruments such as a microscope in a specialized laboratory,
Japanese Patent Publication No. 6-83624 discloses a method of combining a sticky substance with a compound which develops a color by reacting with a body fluid of a tick. There is a method of detecting the body fluid of mites and excreted urine of mites by performing a color reaction with a diazo compound. However, in the combination of the viscous substance and the compound that develops a color by reacting with the body fluid of the tick, the body fluid of the tick is too small to be visually confirmed, and cannot be visually recognized unless it is enlarged by a microscope or the like. It is as complicated as directly observing mites with a microscope. Further, in a method of causing a color reaction of household dust with an aromatic diazo compound, a strongly alkaline solution must be used, which poses a safety problem for household goods.

[0006] Furthermore, these methods can detect mite allergens, but cannot detect protein indoor dust, which is a tick's prey. Could not be determined. Thus, it has been desired to safely and easily detect protein indoor dust serving as mite allergens and mites.

[0007]

SUMMARY OF THE INVENTION In view of the above, an object of the present invention is to provide a kit for detecting protein indoor dust, which can safely and easily detect protein indoor dust serving as mite allergens and mites.

[0008]

The present invention provides a fiber assembly,
Further, the present invention is a kit for detecting dust in a protein room, which comprises a compound which forms a color by reacting with a protein as an essential component. Hereinafter, the present invention will be described in detail.

The kit for detecting dust in a protein room according to the present invention comprises a fiber assembly and a compound which forms a color by reacting with the protein as essential components. The fiber aggregate is not particularly limited as long as it is made of a fibrous substance having a function of collecting indoor dust, and examples thereof include a woven fabric and a nonwoven fabric. The method for producing the nonwoven fabric is not particularly limited, and examples thereof include a heat fusion method and a compression method.

The material of the fiber assembly may be, for example,
Thermoplastic fibers such as polyester fibers, polyamide fibers, and polyolefin fibers, composite fibers of these fibers, semi-synthetic fibers such as acetate, recycled fibers such as cupra and rayon, natural fibers such as cotton, and cotton blends of these. Is mentioned. In addition, these fibers and fiber aggregates,
The dust collection function may be improved by forming an electric field by a surface treatment with a surfactant or an oil agent and a polarization treatment.

In the present invention, since the fiber aggregate is used, the collected indoor dust is fixed on the fiber surface, so that the color forming operation can be easily performed as it is, and the color formation can be easily visually confirmed. Generally, in order to collect indoor dust,
Other than using the fiber aggregate, a method using an apparatus such as a vacuum cleaner, a method using a broom or the like, a method using an adhesive, and the like are conceivable. However, in the case of using a vacuum cleaner, it is necessary to take extra labor to take out the indoor dust collected in the apparatus in order to develop a color, and there is a problem in hygiene such as spilling when taking out. Further, even when the dust is swept up with a broom or the like, it is necessary to fix it to something in order to make the indoor dust color and confirm it, which is not practical. Furthermore, when an adhesive is used, the surface of an object to be cleaned, for example, a tatami mat or a futon, may be peeled off by the adhesive and may be damaged. The fiber aggregate may be used not only for collecting dust in the protein room for detection but also as a cleaning tool.

The compound that forms a color by reacting with the above-mentioned protein is not particularly limited. For example, ninhydrin, 2,4,4
6-trinitrobenzenesulfonic acid, amide black 10, acid orange 12, buffalo black,
Orange G, methyl orange, bromophenol blue, azosulfathiazole, safranine and the like. Above all, the change in color can be easily determined with the naked eye,
Ninhydrin is preferred. The coloring reaction may be promoted by heating or adding a catalyst.

In the present invention, the compound which develops a color by reacting with a protein is preferably contained in a carrier which is separated from the fiber assembly. Protein indoor dust also includes human skin, and compounds that react and develop color with the above proteins also develop human skin. There is a risk of color development. Therefore, for example, a method of impregnating a carrier with a compound that develops a color by reacting with a protein, collecting indoor dust with a fiber aggregate, and then contacting the indoor dust fixed to the fiber aggregate with the carrier is exemplified. Can be The carrier is not particularly limited.For example, paper, fiber aggregates such as nonwoven fabric, zeolite, silica, inorganic carriers such as clay minerals, inorganic gels, starch, agar, polyacrylates and the like are swollen in a solvent. Organic gels and the like can be mentioned. Among these carriers, organic gels are preferably used.

The kit of the present invention detects protein room dust. The above-mentioned protein dust is not particularly limited, and examples thereof include proteins such as hair of humans and animals, flakes of skin such as dandruff, pollen, mold and food waste. To detect protein indoor dust means to confirm the presence or absence of protein indoor dust.

The form of the kit for detecting dust in a protein room of the present invention is not particularly limited. For example, a kit comprising a sheet-like fiber aggregate and a spray containing a compound which reacts with the protein to form a color, or a sheet A kit comprising a compound which develops a color by reacting with the above-mentioned protein impregnated in a fiber-like fiber aggregate and a sheet-like carrier. In addition, in the kit, apart from a compound that develops a color by reacting with the protein,
An organic solvent or a carrier for dissolution is contained, and the carrier may be dissolved in the organic solvent to form a spray when used or impregnated in the carrier. Further, the kit for detecting dust in a protein room of the present invention may include other components. The other elements are not particularly limited, and include, for example, an organic solvent for dissolution, a container for dissolution, an unimpregnated carrier, and gloves for preventing coloration of the skin of hands.

As a mode of use of the kit for detecting indoor protein dust of the present invention, the surface of a carpet or a tatami mat of a general household is rubbed with the above-mentioned fiber assembly to collect indoor dust, and the collected indoor dust is mixed with the collected indoor dust. And a compound that develops a color by reacting with a compound. The compound that develops a color by reacting with the protein is 0.001 to water or an organic solvent.
It is preferable to use it after dissolving it to a concentration of about 20%. The dissolution with water or an organic solvent may be performed in advance or at the time of use. When a compound that develops a color by reacting with the protein is dissolved in water or an organic solvent, it is preferable to store the compound in a closed container so as not to dry in consideration of long-term storage. As a method of contacting and reacting the compound that forms a color by reacting with the protein, and a method of contacting and reacting with the indoor dust, a method of spraying a diluted substance of the compound that forms a color by reacting with the protein and spraying the indoor dust, or a method of reacting with the protein A method in which a sealed carrier is impregnated with a diluted substance of a compound that forms a color to form a sealed color body and is brought into contact with dust in a room.

The kit for detecting dust in a protein room according to the present invention contains, as essential components, a fiber assembly and a compound which develops a color by reacting with the protein. Therefore, not only mite allergens but also protein indoor dust can be detected, and it can be determined whether or not it is a situation in which ticks are easy to breed.

[0018]

The present invention will be described in more detail with reference to the following examples, but the present invention is not limited to these examples.

Example 1 Tremicron EM030 (manufactured by Toray Industries, Inc.) as a fiber assembly
Was cut into 20 cm x 20 cm, and rubbed the carpet of the living room of the T family, the carpet of the study room of the S family, and the carpet of the entrance of the M family.
Each room dust was collected. Trenin micron EM030 in which collected indoor dust is fixed using ninhydrin spray (manufactured by Wako Pure Chemical Industries, Ltd.) as a compound that develops a color by reacting with proteins.
And the degree of color development was visually confirmed. Table 1 shows the coloring results.

COMPARATIVE EXAMPLE 1 A home vacuum cleaner (Maihime 510, manufactured by Sanyo Electric Co., Ltd.) was used to clean a carpet in the living room of the house T, a carpet in the study of the house S, and a carpet in the entrance of the house M. 1 m ² was cleaned over 1 minute to collect indoor dust. Take out the dust collection bag of the household vacuum cleaner, perform the Acarex test (manufactured by Allergo Pharma), a detection kit that measures the amount of guanine and estimate the number of breeding mites, and determine the amount of feces and dead carcasses. Judged. Table 1 shows the results of each determination.

COMPARATIVE EXAMPLE 2 A home vacuum cleaner (Maihime 510, manufactured by Sanyo Electric Co., Ltd.) was used to clean the carpet of the living room T, which is an ordinary household, the carpet of the study of the house S, and the carpet of the entrance of the house M. 1 m ² was cleaned over 1 minute to collect indoor dust. The dust collection bag of the household vacuum cleaner was taken out, the mite bodies were separated from the indoor dust, and observed with a microscope. Table 1 shows the measurement results.

[0022]

[Table 1]

Example 2 Polyester nonwoven fabric as a fiber aggregate, P080JK
900 (manufactured by Toyobo Co., Ltd.) cut into 20 cm x 20 cm, and rubbed each surface of the two tatami mats in the bedroom of the S family, which is a general household, and the tatami mat of the study in the N family, to catch each indoor dust. Gathered. A solution was prepared by dissolving 0.01 g of ninhydrin (manufactured by Wako Pure Chemical Industries, Ltd.) in a mixed solvent of 1 mL of water and 1 mL of ethanol as a compound that develops a color by reacting with a protein. Furthermore,
The prepared ninhydrin-water and ethanol solution was 5 cm ×
Ecule 615 polyester nonwoven fabric cut to 5 cm
1A (manufactured by Toyobo Co., Ltd.) and affixed to a transparent packing tape (manufactured by 3M) to produce a seal-like colored body. Non-woven fabric P in which colored body is fixed to collected indoor dust
080JK900, and the degree of color development was visually checked. Table 2 shows the coloring results.

Comparative Example 3 Two tatami mats in the bedroom of the S family, which is a general home, and a tatami mat in the study of the N family were squeezed by a home vacuum cleaner (Maihime 510, manufactured by Sanyo Electric Co., Ltd.) for about 1 minute to obtain 1 m ² . Cleaned and collected indoor dust. The dust collection bag of the household vacuum cleaner was taken out, the mite bodies were separated from the indoor dust, and observed with a microscope. Table 2 shows the measurement results.

[0025]

[Table 2]

[0026]

Industrial Applicability According to the present invention, it becomes easy to detect mite allergens and protein dust that becomes a bait for mites. In addition, it has become possible to determine whether or not the location where the detection has been performed is in a state in which ticks are likely to breed, so that efficient cleaning has become possible.

Claims (3)

[Claims] 1. A kit for detecting dust in a protein interior, comprising a fiber assembly and a compound which forms a color by reacting with a protein as an essential component. 2. The kit for detecting dust in a protein room according to claim 1, wherein the compound that forms a color by reacting with the protein is contained in a carrier separated from the fiber assembly. 3. The kit for detecting dust in a protein room according to claim 1, wherein the compound that develops a color by reacting with the protein is ninhydrin.