JP2002322078A - Skin care preparation - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a skin care preparation controlling complicated skin inflammatory reactions, or the like, having synergistically improved preventing and ameliorating effects on chapped skin and damage and aging of the skin. SOLUTION: This skin care preparation is obtained by adding one or more kinds selected from an extract and an extraction fraction of Puerariae Radix and a glycogen to a base for skin care preparation. When the extract is adsorbed on an ion-exchange adsorption resin and successively eluted with a water/ethanol mixed solvent, a fraction obtained by elution with 50 vol.% to 99.5 vol.% aqueous solution of ethanol is preferably used as the extraction fraction. 4',7-Dihydroxyisoflavone and a glycogen may be used. A glycogen derived from Mytilus edulis is preferable as the glycogen.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an external preparation for skin which synergistically improves the effects of preventing and improving rough skin, skin damage and aging. More specifically, one or two or more selected from extracts and extract fractions of cuckoo, or 4 ', 7-dihydroxyisoflavone which is an active ingredient thereof,
The present invention relates to an external preparation for skin which is used in combination with glycogen.

[0002]

2. Description of the Related Art Due to aging, exposure to ultraviolet rays, oxidative stress due to reactive oxygen species generated in skin tissues, contact with drugs and various allergens, skin inflammatory reactions, skin damage and aging progress. It is known. In the field of skin external preparations, many physiologically active ingredients such as active oxygen species scavengers, anti-inflammatory agents and anti-allergic agents have been searched and examined in order to prevent such skin inflammation and aging. In recent years, there has been a strong tendency to seek such components from plants, reflecting the natural and plant consciousness of consumers.

[0003] However, among the above-mentioned components of plant origin which have already been reported, due to their low activity, a fairly high concentration is required to obtain a sufficient effect when incorporated into a skin external preparation, There is a problem in terms of stability and safety, and there is something that gives an undesired color or odor to the external preparation for skin. At present, there are few things that can be satisfied in all aspects. In addition, the skin inflammatory reaction, damage, aging, and the like progress due to various factors involved in a complex manner, and thus, even if a substance that acts only on a part of the reaction is not sufficiently effective. .

[0004]

Therefore, in the present invention, an external preparation for skin which suppresses complex skin inflammatory reactions and the like, and has a synergistically improved effect of preventing and improving skin roughness, skin damage and aging is obtained. It was aimed at.

[0005]

The present inventors have made various studies to solve the above-mentioned problems, and as a result, the present inventors have found that Pueraria
e Radix ) extract and extract have high fibroblast activating and collagen production-promoting effects, and one or more selected from these, combined with glycogen, are contained in a skin external preparation. By doing so, it was found that a good anti-inflammatory action and a skin repair action were obtained, and a synergistic improvement in the effects of preventing and improving skin roughness, skin damage and aging was obtained, and completed the present invention. .

[0006] In particular, when a high content of 4 ', 7-dihydroxyisoflavone (daidzein) is used as the cuckoo extract and extract fraction, a good effect can be obtained.
It may be used in combination with 4 ', 7-dihydroxyisoflavone itself.

[0007] In addition, the extract of cucumbers, mucopolysaccharide fragmentation suppression, active oxygen scavenging, antioxidant activity (Japanese Unexamined Patent Publication No.
24937), a fat synthesis promoting action (JP-A-11-199)
499), dermal papilla activating action (JP-A-11-24082)
No. 3) has been disclosed, and a hyaluronic acid production promoting action has also been reported (Abstracts of the 120th Annual Meeting of the Pharmaceutical Society of Japan, No. 58)
Page, 2000), but nothing is known about the type I collagen production promoting action. Furthermore, a metabolite of 7-isopropoxy isoflavone has been reported to promote the synthesis of type I collagen (Calcified Tissue Int.
Although it is well known that E. ernational 55 (5) 356-362 (1994) has an antiacetylcholine effect with respect to 4 ', 7-dihydroxyisoflavone in cuckoo extract, such a report has not been made so far.

[0008] Glycogen is used as a humectant (Japanese Patent Application Laid-Open No. 62-178505,
290809) and a fibroblast proliferation action (Japanese Unexamined Patent Application Publication No.
255657). However,
The effect of the present invention obtained by using a combination of glycogen and an extract or extract fraction of cuckoo or 4 ', 7-dihydroxyisoflavone has not been suggested yet.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION Cuckoo ( Puerariae Radix ) used in the present invention is a root excluding the pericarp of kudzu ( Pueraria lobata Ohwi), which is a deciduous fujimoto belonging to the leguminous family ( Leguminosae ), and is produced in Japan and Korea. And square brackets, board brackets and powdered brackets produced in China can be used.

[0010] Cuckoo ( Puerariae Radix ) may be subjected to extraction as it is, but in consideration of extraction efficiency, it must be chopped,
It is preferable to perform extraction after performing processing such as drying and pulverization. The extraction is performed by immersion in an extraction solvent. Stirring may be performed to increase the extraction efficiency, or homogenization may be performed in an extraction solvent. It is appropriate that the extraction temperature is set to a temperature of about 5 ° C. to the boiling point of the extraction solvent or lower. The extraction time varies depending on the type of the extraction solvent and the extraction temperature, but is from 4 hours to 1 hour.
It is appropriate to make it about four days.

As an extraction solvent, in addition to water, methanol,
Lower alcohols such as ethanol, propanol and isopropanol; polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin; ethers such as ethyl ether and propyl ether; esters such as ethyl acetate and butyl acetate And polar organic solvents such as ketones such as acetone and ethyl methyl ketone, and one or more of these can be selected and used. Further, physiological saline, phosphate buffer, phosphate buffered saline, or the like may be used.

The extract of Puerariae Radix with the above-mentioned solvent can be contained in the external preparation for skin according to the present invention as it is, but the concentrated and dried extract can be redissolved in water or a polar solvent. Alternatively, it may be used after performing a purification treatment such as decolorization, deodorization, desalting or the like within a range that does not impair these physiological actions, or after performing a fractionation treatment by column chromatography or the like. In particular, after being adsorbed on an ion exchange adsorption resin, when sequentially eluted with a mixed solvent of water and ethanol, the fraction eluted with a 50% to 99.5% by volume aqueous ethanol solution contains 4 ′, 7-dihydroxyisoflavone. It is most contained and is most preferably used in terms of collagen production promoting action. As the ion exchange resin, DI
AION MCI gel HP-20 (manufactured by Mitsubishi Chemical Corporation) or the like is preferably used. The extract of the parentheses, the processed product thereof, and the fractionated product may be freeze-dried after each treatment and fractionation, and may be used by dissolving in a solvent at the time of use. Also,
It can also be used by being encapsulated in vesicles such as liposomes or microcapsules.

In the present invention, purified 4 ', 7-dihydroxyisoflavone may be used in place of the above-mentioned extract of cuckoo or a fraction thereof.

In the present invention, the parentheses ( Pueraria)
e Radix ), glycogen used in combination with one or more of the extract and extract fraction, or in combination with 4 ', 7-dihydroxyisoflavone, is a glucan by α-1,6 bond to α-1,4-glucan chain. Is a homopolysaccharide having the branches of animal, liver, muscle, mussel shells such as mussels, corn seeds,
Extracted and prepared from rice grains, yeast, etc. can be used. Alternatively, a commercially available product based on the above-mentioned animals and plants may be used. Among them, mussels ( Myti lus ed
Glycogen extracted from mussels such as ulis ) is most preferably used since it has an excellent skin damage repair effect.

Glycogen is extracted by slicing or pulverizing tissues of animals and plants and immersing them in purified water. After immersion in purified water, it may be crushed by a colloid crusher or an ultrasonic crusher. After removing the protein by heat treatment and / or treatment with trichloroacetic acid, etc., the extract obtained by centrifugation,
Glycogen is precipitated by adding an organic solvent such as methanol, ethanol, or acetone, or concentrated.
Glycogen can be recovered by spraying and drying.

In the present invention, the cucumber ( Puerariae)
One or more extracts and extract fractions of Radix ),
Alternatively, 4 ′, 7-dihydroxyisoflavone and glycogen are contained in a skin external preparation base. For the total amount of skin preparation for external use, parentheses ( Puerariae Radi
The extract or extract fraction of x ) differs depending on the preparation method, but is about 0.0001 to 5.0% by weight,
0.00001 for dihydroxyisoflavone
About 0.1% by weight, and about 0.001 for glycogen
It is appropriate that the content be about 1 to 5.0% by weight.

The external preparation for skin according to the present invention can be provided in various dosage forms such as lotions, emulsions, gels, creams, ointments, powders, granules and the like. Also, skin cosmetics such as lotion, milky lotion, cream, serum, pack, etc.
Base cosmetics such as makeup base lotion and makeup base cream, foundations of various dosage forms such as milky liquid, oily and solid, makeup cosmetics such as eye color and cheek color, hand cream, leg cream, neck cream , Body lotions and the like.

The external preparation for skin according to the present invention includes, in addition to the extract of Puerariae Radix, glycogen, oily components, surfactants, humectants, pigments, ultraviolet absorbers, antioxidants, fragrances, Raw materials for general pharmaceuticals and cosmetics such as antibacterial and fungicides, and physiologically active ingredients such as skin cell activators, anti-inflammatory agents, and whitening agents can be contained.

[0019]

EXAMPLES Further, the features of the present invention will be described in detail with reference to examples.

First, preparation of glycogen ( Puerariae Radix ) extract and extract fraction to be contained in the external preparation for skin according to the present invention will be described.

[Cuckold extract 1] 200 g of dried cuckoo powder was immersed in 1 liter of ethanol, and allowed to stand at 20 ° C. for 7 days to extract. The extract was collected by filtration, concentrated, and dried. Lyophilized to give the title parentheses extract.

[Cuckold extract 2] 500 g of cuckoo is cut into small pieces, extracted with stirring in 2 liters of ethanol at 20 ° C. for 3 days, and the extract is collected by filtration, concentrated and dried.
It was dissolved in 1 liter of glycerin to obtain the title extract of parentheses.

[Brack extract 3] 250 g of bracken extract were dried and pulverized, immersed in 2 liters of a 50% by volume aqueous ethanol solution at 20 ° C. for 7 days, and then filtered to collect a filtrate. did.

[Cuckon Extraction Fractions 1-4] 500 g of Puerariae Radix was dried, pulverized, immersed in 2 liters of ethanol, allowed to stand at 20 ° C. for 7 days, extracted, and filtered. The liquid is recovered, then concentrated, dried and freeze-dried. 36.0 g of this dry powder was dissolved in 500 ml of ethanol, and 1500 ml of purified water was added, and 600 ml of DIAION MCI gel HP-20 was added.
(Mitsubishi Chemical Corporation) and stirred for 1 hour,
The resin was collected by filtration, packed in a column, and eluted stepwise with a mixed solvent of water and ethanol. that time,
50 volume% ethanol aqueous solution, 70 volume% ethanol aqueous solution, 90 volume% ethanol aqueous solution and 99.5 volume%
Fractions eluted with an aqueous ethanol solution were collected and freeze-dried, respectively, to obtain fractionated extracts 1 to 4.

[Murasaki mussel-derived glycogen] 10 kg of mussel mussel ( Mytilus edulis ) shellfish meat and hot water 5
After adding the liter and leaving the mixture to stand for 15 minutes, the mixture is crushed by a colloid crusher. After allowing the crushed liquid to cool, at 4 ° C.
After centrifuging at 000 rpm for 5 minutes, trichloroacetic acid was added to the supernatant to 5% by weight to precipitate the protein, followed by filtration. The filtrate was further sterilized by a membrane filter, concentrated, and then concentrated by a spray drier. It was obtained by spraying and drying.

[Glycogen derived from corn seed] 1 kg of corn ( Zea mays L.) seeds are ground with a grinder, 1 kg of purified water is added and mixed, and the mixture is stirred and extracted at room temperature for 15 minutes. The extract is filtered and the filtrate is 8,500 rpm
The supernatant was heat-treated at 95 ° C. for 20 minutes, cooled, and centrifuged at 5,000 rpm for 5 minutes to remove the coagulated protein. Cool the supernatant to 4 ° C,
The protein was precipitated by adding trichloroacetic acid to 5% by weight and filtered. The filtrate was poured into three times the volume of methanol, and the resulting precipitate was collected by centrifugation at 5,000 rpm for 5 minutes. After washing with methanol, ethanol and diethyl ether successively, it was obtained by freeze-drying.

[Rice-derived collagen] 1 kg of polished rice is crushed by a crusher, and 10 liters of purified water is added thereto.
Stir and extract for 5 minutes. The extract is filtered and the filtrate is treated with 8,50.
After centrifugation at 0 rpm for 5 minutes, the supernatant was heat-treated at 95 ° C. for 15 minutes, cooled, and centrifuged at 4,000 rpm for 5 minutes to remove the coagulated protein. The supernatant was cooled to 4 ° C., and the protein was precipitated by adding trichloroacetic acid until the amount became 5% by weight, and the protein was filtered. The filtrate was poured into three times the volume of ethanol, and the resulting precipitate was collected at 5,000 rpm. It was collected by centrifugation for 5 minutes, washed sequentially with methanol, ethanol and diethyl ether, and then freeze-dried to obtain.

Next, the formulations of the examples of the external preparation for skin according to the present invention will be shown.

[Example 1] Lotion agent (1) Ethanol 20.00 (% by weight) (2) Polyoxyethylene (60E.O.) hydrogenated castor oil 1.00 (3) Cuckoo extract 1 0.05 ( 4) Mussel mussel-derived glycogen 0.20 (5) Methyl paraoxybenzoate 0.10 (6) Dipropylene glycol 5.00 (7) 1,3-butylene glycol 10.00 (8) Purified water 63.65 Production method: ( Add (2) and (3) to 1) and dissolve to obtain alcohol phase. On the other hand, (4) to (7) are sequentially dissolved in (8) to form an aqueous phase. Add the alcohol phase to the aqueous phase, stir and mix.

Example 2 Emulsion (1) Cetanol 1.00 (% by weight) (2) Beeswax 0.50 (3) Vaseline 2.00 (4) Squalane 6.00 (5) Dimethylpolysiloxane 2.00 (6) polyoxyethylene (20E.O.) sorbitan 1.00 monostearate (7) glyceryl monostearate 1.00 (8) glycerin 4.00 (9) 1,3-butylene glycol 4.00 (10) corn seed-derived glycogen 0.10 (11) methyl paraoxybenzoate 0.10 (12) purified water 62.28 (13) carboxyvinyl polymer 10.00 (1.0% by weight aqueous solution) (14) hydroxylation Potassium (10.0% by weight aqueous solution) 1.00 (15) Ethanol 5.00 (16) Bracken extract fraction 10.02 Production method: The oil phase components of (1) to (7) It was combined and heated to dissolve 75
° C. On the other hand, the aqueous phase components (8) to (12) are mixed and dissolved to 75 ° C. After the oil phase was added thereto and pre-emulsified, (13) was added thereto, and the mixture was uniformly emulsified with a homomixer, and then (14) was added to increase the viscosity, followed by cooling. To
Dissolve in (15), add and mix.

Example 3 Oil-in-Water Cream (1) Beeswax 6.00 (% by weight) (2) Cetanol 5.00 (3) Reduced Lanolin 8.00 (4) Squalane 27.50 (5) Glyceryl Fatty acid ester 4.00 (6) Lipophilic glyceryl monostearate 2.00 (7) Polyoxyethylene (20E.O.) sorbitan 5.00 Monolaurate (8) Propylene glycol 5.00 (9) Paraoxy Methyl benzoate 0.10 (10) Cuckon extract fraction 2 0.02 (11) Rice-derived glycogen 0.25 (12) Purified water 37.13 Production method: Mix oil phase components (1) to (7) Dissolve to 75 ° C. On the other hand, (9) and (10) are dissolved in (8) and (12)
, Mixed and dissolved, and heated to 75 ° C. Next, the oil phase component is added to the aqueous phase component, preliminarily emulsified, then uniformly emulsified by a homomixer, and cooled.

Example 4 Gel (1) Dipropylene glycol 10.00 (% by weight) (2) Carboxyvinyl polymer 0.50 (3) Potassium hydroxide (10.0% by weight aqueous solution) 1.00 ( 4) Methyl paraoxybenzoate 0.10 (5) Cracker extract fraction 3 0.02 (6) Mussel mussel-derived glycogen 0.15 (7) Purified water 88.23 Production method: (2), (6) ) Is dissolved uniformly, then (1) to (4),
(5) is dissolved and added, and then (3) is added to increase the viscosity.

[Example 5] Oil-in-water emulsion ointment (1) White petrolatum 25.0 (wt%) (2) Stearyl alcohol 25.0 (3) Glycerin 12.0 (4) Sodium lauryl sulfate 1.0 (5) Glycogen derived from mussels 0.1 (6) Glycogen derived from corn 0.1 0.1 (7) Methyl paraoxybenzoate 0.1 (8) Purified water 35.2 (9) 1.0 of extract 4 of cuckoo extract (w / v)% 1.5 Ethanol solution Production method: Mix and heat the oil phase components of (1) to (4) to uniformly dissolve, and bring to 75 ° C. On the other hand, the aqueous phase components (5) to (8) are mixed,
Heat to 75 ° C. The oil phase component was gradually added to the water phase component with stirring to emulsify, and after cooling, 40
Add (9) at ℃ and mix.

Example 6 Liposome preparation (1) Glycerin 2.0 (% by weight) (2) 1,3-butylene glycol 3.0 (3) Polyoxyethylene (25E.O.) oleyl ether 0.2 (4) Ethanol 10.0 (5) Methyl parahydroxybenzoate 0.1 (6) Purified water 79.7 (7) 4 ', 7-dihydroxyisoflavone, Murasaki 5.0 Mussel-derived liposome-encapsulated liposome Preparation method: (5) Is dissolved in (4), added to (6) together with (1) to (3), mixed uniformly, and dispersed by adding (7) thereto. The glycogen-encapsulated liposomes derived from 4 ′, 7-dihydroxyisoflavone and mussels in (7) are obtained by mixing 50 ml of a 50% by volume aqueous ethanol solution containing 1.0 (w / v)% of 4 ′, 7-dihydroxyisoflavone with mussels derived from mussels. 80 g of soybean lecithin was added to 50 ml of a 2.5% (w / v) aqueous solution of glycogen, suspended at 55 ° C., and then sonicated to prepare liposomes, which were recovered by centrifugation.

[Example 7] Water-in-oil emollient cream (1) Liquid paraffin 30.00 (% by weight) (2) Microcrystalline wax 2.00 (3) Vaseline 5.00 (4) Diglyceryl dioleate Ester 5.00 (5) Sodium L-glutamate 1.60 (6) L-Serine 0.40 (7) Propylene glycol 3.00 (8) Methyl parahydroxybenzoate 0.10 (9) Cuckoo extract 20. 50 (10) Glycogen derived from rice 0.02 (11) Purified water 52.28 (12) Fragrance 0.10 Production method: Dissolve (5) and (6) in a part of (11) and bring to 50 ° C. The mixture is gradually added to (4) heated to 50 ° C. while stirring. This is mixed in advance, and is uniformly dispersed in (1) to (3) heated and dissolved at 70 ° C. In addition, (8), (9)
And added to the remainder of (11) together with (10), and the mixture heated to 70 ° C. is added with stirring and emulsified by a homomixer. After cooling, (12) is added and mixed at 40 ° C.

Example 8 Makeup Base Cream (1) Stearic acid 12.00 (% by weight) (2) Cetanol 2.00 (3) Glyceryl tri-2-ethylhexanoate 2.50 (4) Self-emulsification Glyceryl monostearic acid 2.00 ester (5) Propylene glycol 10.00 (6) Corn seed-derived glycogen 0.02 (7) Potassium hydroxide 0.30 (8) Methyl parahydroxybenzoate 0.10 (9) Purification Water 67.48 (10) Titanium oxide 2.00 (11) Bengala 0.40 (12) Yellow iron oxide 0.10 (13) Fragrance 0.10 (14) Cuckon extract 3 1.00 Production method: (1) Mix and dissolve the oil phase components of (4) to (75). On the other hand, the aqueous phase components (5) to (9) are mixed and dissolved by heating, and the pigment components (10) to (12) are added thereto and uniformly dispersed by a homomixer to 75 ° C. Next, the oil phase component is added to the water phase component, and the mixture is uniformly emulsified by a homomixer. After cooling, (13) and (14) are added and mixed at 40 ° C.

Example 9 Emulsion Foundation (1) Stearic acid 2.00 (% by weight) (2) Squalane 5.00 (3) Octyldodecyl myristate 5.00 (4) Cetanol 1.00 (5) Decaglyceryl monoisopalmitate 9.00 (6) 1,3-butylene crychol 6.00 (7) Methyl parahydroxybenzoate 0.10 (8) Cuckon extract 1 0.01 (9) Rice-derived glycogen 0 0.05 (10) Potassium hydroxide 0.08 (11) Purified water 53.51 (12) Titanium oxide 9.00 (13) Talc 7.40 (14) Bengala 0.50 (15) Yellow iron oxide 1.10 (16) Black iron oxide 0.10 (17) Fragrance 0.15 Production method: Mix and dissolve oil phase components (1) to (5) to 75 ° C. On the other hand, (7) and (8) are dissolved in (6) and (9) and (10)
In addition to (11), mix and heat dissolve, add the pigment components of (12) to (16) and uniformly disperse with a homomixer to 75
° C. Next, the oil phase component was added to the aqueous phase component, and the mixture was uniformly emulsified with a homomixer.
Add and mix (17).

Example 10 Hand Cream (1) Cetanol 4.00 (% by weight) (2) Vaseline 2.00 (3) Liquid paraffin 10.00 (4) Glyceryl monostearate 1.50 (5) Polyoxyethylene (60E.O.) glyceryl 2.50 Isostearate (6) Tocopherol acetate 0.25 (7) Glycerin 20.00 (8) Methyl parahydroxybenzoate 0.10 (9) Cuckon extract fraction 1 0.02 (10) Mussel mussel-derived glycogen 0.05 (11) Purified water 59.58 Production method: Mix and dissolve oil phase components (1) to (6) to 75 ° C. On the other hand, (8) and (9) are dissolved in (7) and (10) together with (11)
Then, mix and dissolve to 75 ° C. Next, the oil phase component is added to the aqueous phase component, uniformly emulsified by a homomixer, and cooled.

Among the above-mentioned examples according to the present invention, the effect of suppressing the formation of wrinkles on the skin by medium wavelength ultraviolet (UVB) was evaluated for Examples 1 to 6. At that time, in Example 1 to Example 6, each of the blended cuckoo extract or fraction and various glycogens, or 4 ′, 7
-Dihydroxyisoflavone and mussel-derived glycogen-encapsulated liposomes were replaced as shown in Table 1 as Comparative Examples 1 to 6 and evaluated simultaneously. For evaluation, five hairless mice were used as one group, and 0.2 g of each of Examples and Comparative Examples for each group was applied to the back once a day, and 100 mJ / cm ² / time of UVB was applied 3 times a week.
Irradiation was performed 20 times for 20 weeks, and the formation of wrinkles on the skin of the hairless mouse was observed. At this time, a group to which only purified water was applied was used as a control. The results were calculated as the average value of each group, and shown in Table 3 in relation to the number of UVB irradiation days.

[0040]

[Table 1]

[0041]

[Table 2]

[0042]

[Table 3]

As is clear from Table 3, in the control, the depth of wrinkles formed on the skin reached a medium level when the number of UVB irradiation days exceeded 10 weeks, and deep wrinkles formed after 20 weeks. Was recognized. Bracken extract fraction 4
Comparative Example 5 in which glycogen derived from mussels and mussel and glycogen derived from corn seed were all replaced with purified water
In the application group, no significant wrinkle formation inhibitory effect was observed compared to the control. Comparative Example 1 to Comparative Example 4 in which any of the extract or extract fraction of cuckoo, glycogen derived from mussel or corn seed derived glycogen was replaced with purified water, and containing liposomes containing only 4 ′, 7-dihydroxyisoflavone In Comparative Example 6 applied group, the formation of slight wrinkles was observed only after 20 weeks, and the formation of wrinkles was well suppressed. However, in the applied group of Examples, the formation of fine wrinkles was observed after 20 weeks. To the extent observed, the suppression effect on wrinkle formation was significantly improved as compared to the corresponding comparative group.

Subsequently, use tests were conducted on Examples 1 to 10 of the present invention to evaluate the effect of improving the aging symptoms of the skin. At that time, in Examples 7 to 10, the blended cuckoo extract or extracted fraction and various glycogens were replaced as shown in Table 4 to make Comparative Examples 7 to 10 and Comparative Examples 1 to 10 described above. A use test was conducted simultaneously with Example 6.

[0045]

[Table 4]

The effect of improving the aging of the skin is from 40s to 60s, in which fine wrinkles are formed and skin elasticity is remarkably reduced.
Twenty-year-old female panelists were grouped into one group, and each group was evaluated using each of the examples and comparative examples twice a day continuously for two months using a blind. The degree of fine wrinkles was evaluated by visual observation and photography, and skin elasticity was measured with a cute meter. The conditions before and after the start of the use test were compared, and the results were "improved", "slightly improved", and "changed". None ". The results are shown in Table 5 by the number of panelists who obtained each evaluation.

[0047]

[Table 5]

As is clear from Table 5, each of the groups used in Comparative Examples except for the group used in Comparative Example 5 except that all of the extract from the cuckoo, glycogen derived from mussel and glycogen derived from corn seed were replaced with purified water. Although almost good wrinkles and improvement tendencies in skin elasticity were also observed, the number of panelists who showed a clear improvement was significantly greater in the group using the examples than in the corresponding groups using the corresponding comparative examples. Was.

Further, the effects of the present invention for improving skin roughness symptoms were evaluated for Examples 1 to 10 and Comparative Examples 1 to 10. The effect of improving the skin roughness symptoms was as follows. Twenty female panelists in their twenties to sixties who exhibited remarkable skin roughness symptoms were grouped into one group, and each group was blinded with each of the Examples and Comparative Examples twice daily. The evaluation was performed by using continuously for months. The skin condition before and after the start of the use test was evaluated and scored according to the evaluation criteria shown in Table 6, and the average value of 20 persons was calculated and shown in Table 7.

[0050]

[Table 6]

[0051]

[Table 7]

As is evident from Table 7, in each of the groups using the examples of the present invention, remarkable improvement in skin roughness was observed, and after the use test, the skin condition was improved to an almost satisfactory state. . On the other hand, in the group using the comparative example, quite good improvement of the skin roughness was observed except for the group using the comparative example 5, but the degree was smaller than that of the corresponding group using the example.

In Examples 1 to 10, 2
When stored at 5 ° C. for 6 months, no change in the condition was observed, and no problematic skin irritation was observed in a 48-hour back obstruction sticking test by 30 male panelists.

[0054]

As described in detail above, according to the present invention, an external preparation for skin having good stability and safety, and synergistically improved prevention and improvement of skin roughness and skin aging can be obtained. .

[Procedure amendment]

[Submission date] September 4, 2001 (2001.9.4)

[Procedure amendment 1]

[Document name to be amended] Statement

[Correction target item name] 0027

[Correction method] Change

[Correction contents]

[0027] [US-derived glycoside Gen] was ground in a crusher polished rice 1 kg, 20 ° C. by adding purified 10 l water
And extract with stirring for 15 minutes. The extract is filtered and the filtrate is 8,
Centrifuge at 500 rpm for 5 minutes.
The mixture was heated for 5 minutes, cooled, and centrifuged at 4,000 rpm for 5 minutes to remove the coagulated protein. 4 supernatant
C., and the protein was precipitated by adding trichloroacetic acid until the weight became 5% by weight, and the protein was filtered. The filtrate was poured into three times the volume of ethanol, and the resulting precipitate was dried at 5,000 rpm for 5 minutes.
The solution was collected by centrifugation for 1 minute, washed sequentially with methanol, ethanol and diethyl ether, and then freeze-dried to obtain.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification code FI Theme coat ゛ (Reference) A61K 31/715 A61K 31/715 35/56 35/56 A61P 29/00 A61P 29/00 43/00 107 43 / 00 107 F-term (reference) 4C083 AA071 AA082 AA111 AB032 AB232 AB242 AB432 AC012 AC022 AC072 AC102 AC122 AC182 AC242 AC352 AC402 AC422 AC432 AC482 AC582 AC782 AC841 AC842 AD092 AD152 AD211 AD212 AD512 AD662 CC05 CC11 CC12 DD22 DD33 DD01 DD08 DD08 MA02 MA04 MA16 MA27 MA63 NA14 ZA89 ZB11 ZB22 4C087 AA01 BB13 CA06 MA02 MA17 MA22 MA24 MA28 MA63 NA14 ZA89 ZB11 ZB22 4C088 AB59 AC11 BA08 CA03 MA04 MA17 MA22 MA24 MA28 MA63 NA14 ZA89 ZB11 ZB22

Claims (4)

[Claims] An external preparation for skin, comprising one or more selected from extracts and extract fractions of cuckoo ( Puerariae Radix ) and glycogen. 2. An extract fraction of cuckoo ( Puerariae Radix ), after the extract is adsorbed on an ion exchange adsorption resin,
The external preparation for skin according to claim 1, characterized in that, when sequentially eluted with a mixed solvent of water and ethanol, the fraction is eluted with 50% by volume to 99.5% by volume of an aqueous ethanol solution. 3. An external preparation for skin comprising 4 ', 7-dihydroxyisoflavone and glycogen. 4. The external preparation for skin according to claim 1, wherein the glycogen is extracted from mussels.