JP2002506204A - 可逆的アフィニティー電気泳動を用いる精製および検出プロセス - Google Patents
可逆的アフィニティー電気泳動を用いる精製および検出プロセスInfo
- Publication number
- JP2002506204A JP2002506204A JP2000534862A JP2000534862A JP2002506204A JP 2002506204 A JP2002506204 A JP 2002506204A JP 2000534862 A JP2000534862 A JP 2000534862A JP 2000534862 A JP2000534862 A JP 2000534862A JP 2002506204 A JP2002506204 A JP 2002506204A
- Authority
- JP
- Japan
- Prior art keywords
- analyte
- electrophoretic medium
- electric field
- affinity ligand
- changed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 87
- 230000002441 reversible effect Effects 0.000 title claims abstract description 11
- 238000000746 purification Methods 0.000 title abstract description 34
- 230000008569 process Effects 0.000 title abstract description 16
- 238000001514 detection method Methods 0.000 title abstract description 12
- 238000002299 affinity electrophoresis Methods 0.000 title abstract description 6
- 239000003446 ligand Substances 0.000 claims abstract description 135
- 239000012491 analyte Substances 0.000 claims abstract description 126
- 238000009739 binding Methods 0.000 claims abstract description 49
- 230000027455 binding Effects 0.000 claims abstract description 48
- 238000001962 electrophoresis Methods 0.000 claims abstract description 37
- 239000000463 material Substances 0.000 claims abstract description 6
- 150000007523 nucleic acids Chemical class 0.000 claims description 78
- 102000039446 nucleic acids Human genes 0.000 claims description 69
- 108020004707 nucleic acids Proteins 0.000 claims description 69
- 230000005684 electric field Effects 0.000 claims description 42
- 230000000295 complement effect Effects 0.000 claims description 26
- 230000008859 change Effects 0.000 claims description 24
- 229920000642 polymer Polymers 0.000 claims description 24
- 108090000623 proteins and genes Proteins 0.000 claims description 22
- 102000004169 proteins and genes Human genes 0.000 claims description 19
- 239000000872 buffer Substances 0.000 claims description 18
- 238000009396 hybridization Methods 0.000 claims description 17
- 239000000203 mixture Substances 0.000 claims description 17
- 239000002773 nucleotide Substances 0.000 claims description 17
- 125000003729 nucleotide group Chemical group 0.000 claims description 17
- 102000004190 Enzymes Human genes 0.000 claims description 14
- 108090000790 Enzymes Proteins 0.000 claims description 14
- 239000000126 substance Substances 0.000 claims description 13
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical group NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 claims description 12
- 238000012360 testing method Methods 0.000 claims description 11
- 239000003795 chemical substances by application Substances 0.000 claims description 9
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 7
- 239000000758 substrate Substances 0.000 claims description 7
- 239000004202 carbamide Substances 0.000 claims description 5
- XSQUKJJJFZCRTK-UHFFFAOYSA-N urea group Chemical group NC(=O)N XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 5
- 108091023037 Aptamer Proteins 0.000 claims description 4
- 108010066717 Q beta Replicase Proteins 0.000 claims description 4
- 150000001720 carbohydrates Chemical class 0.000 claims description 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 3
- 230000005679 Peltier effect Effects 0.000 claims description 2
- 150000001413 amino acids Chemical class 0.000 claims description 2
- 239000002738 chelating agent Substances 0.000 claims description 2
- 239000005515 coenzyme Substances 0.000 claims description 2
- 239000003814 drug Substances 0.000 claims description 2
- 229940079593 drug Drugs 0.000 claims description 2
- 229940088598 enzyme Drugs 0.000 claims description 2
- 239000002532 enzyme inhibitor Substances 0.000 claims description 2
- 239000007850 fluorescent dye Substances 0.000 claims description 2
- 150000004676 glycans Chemical class 0.000 claims description 2
- 239000005556 hormone Substances 0.000 claims description 2
- 229940088597 hormone Drugs 0.000 claims description 2
- -1 inorganic molecules Substances 0.000 claims description 2
- 150000002632 lipids Chemical class 0.000 claims description 2
- 150000002772 monosaccharides Chemical class 0.000 claims description 2
- 229920001282 polysaccharide Polymers 0.000 claims description 2
- 239000005017 polysaccharide Substances 0.000 claims description 2
- 102000002260 Alkaline Phosphatase Human genes 0.000 claims 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- 238000013508 migration Methods 0.000 claims 1
- 230000005012 migration Effects 0.000 claims 1
- 239000002777 nucleoside Substances 0.000 claims 1
- 150000003833 nucleoside derivatives Chemical class 0.000 claims 1
- 238000000926 separation method Methods 0.000 abstract description 29
- 230000000737 periodic effect Effects 0.000 abstract description 4
- 239000000499 gel Substances 0.000 description 59
- 239000000523 sample Substances 0.000 description 54
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 45
- 239000000306 component Substances 0.000 description 25
- 241000588724 Escherichia coli Species 0.000 description 19
- 238000010494 dissociation reaction Methods 0.000 description 16
- 230000005593 dissociations Effects 0.000 description 15
- 230000037230 mobility Effects 0.000 description 11
- 239000000047 product Substances 0.000 description 11
- 108020004414 DNA Proteins 0.000 description 9
- 229920002401 polyacrylamide Polymers 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 7
- 238000011160 research Methods 0.000 description 7
- 230000009870 specific binding Effects 0.000 description 7
- 229920000936 Agarose Polymers 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 6
- 238000003556 assay Methods 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 6
- 238000012937 correction Methods 0.000 description 6
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- 238000005251 capillar electrophoresis Methods 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- 239000013612 plasmid Substances 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 108020004465 16S ribosomal RNA Proteins 0.000 description 4
- 238000010521 absorption reaction Methods 0.000 description 4
- 239000013578 denaturing buffer Substances 0.000 description 4
- 238000000502 dialysis Methods 0.000 description 4
- 239000000178 monomer Substances 0.000 description 4
- 108020004418 ribosomal RNA Proteins 0.000 description 4
- 238000007873 sieving Methods 0.000 description 4
- 238000013518 transcription Methods 0.000 description 4
- 230000035897 transcription Effects 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 3
- 235000014633 carbohydrates Nutrition 0.000 description 3
- 239000002131 composite material Substances 0.000 description 3
- 239000000975 dye Substances 0.000 description 3
- 238000001502 gel electrophoresis Methods 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 238000002955 isolation Methods 0.000 description 3
- 230000000670 limiting effect Effects 0.000 description 3
- 230000001404 mediated effect Effects 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 102000004196 processed proteins & peptides Human genes 0.000 description 3
- 230000003252 repetitive effect Effects 0.000 description 3
- 241000894007 species Species 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- VHTUHGNVVZPWGO-UHFFFAOYSA-N 7-(2-hydroxyethyl)-1,3-dimethyl-8-(pyridin-3-ylmethyl)purine-2,6-dione Chemical compound OCCN1C=2C(=O)N(C)C(=O)N(C)C=2N=C1CC1=CC=CN=C1 VHTUHGNVVZPWGO-UHFFFAOYSA-N 0.000 description 2
- 229920002307 Dextran Polymers 0.000 description 2
- 229920000663 Hydroxyethyl cellulose Polymers 0.000 description 2
- 239000004354 Hydroxyethyl cellulose Substances 0.000 description 2
- 108090001090 Lectins Proteins 0.000 description 2
- 102000004856 Lectins Human genes 0.000 description 2
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 101710137500 T7 RNA polymerase Proteins 0.000 description 2
- 239000012163 TRI reagent Substances 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 238000001261 affinity purification Methods 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 239000013592 cell lysate Substances 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 125000003636 chemical group Chemical group 0.000 description 2
- 239000002826 coolant Substances 0.000 description 2
- MGNCLNQXLYJVJD-UHFFFAOYSA-N cyanuric chloride Chemical compound ClC1=NC(Cl)=NC(Cl)=N1 MGNCLNQXLYJVJD-UHFFFAOYSA-N 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 2
- 238000007429 general method Methods 0.000 description 2
- 239000011544 gradient gel Substances 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 235000019447 hydroxyethyl cellulose Nutrition 0.000 description 2
- 239000002523 lectin Substances 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 150000003141 primary amines Chemical group 0.000 description 2
- 102000005962 receptors Human genes 0.000 description 2
- 108020003175 receptors Proteins 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- YNDXUCZADRHECN-JNQJZLCISA-N triamcinolone acetonide Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@H]3OC(C)(C)O[C@@]3(C(=O)CO)[C@@]1(C)C[C@@H]2O YNDXUCZADRHECN-JNQJZLCISA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- OSBLTNPMIGYQGY-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetic acid;boric acid Chemical compound OB(O)O.OCC(N)(CO)CO.OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O OSBLTNPMIGYQGY-UHFFFAOYSA-N 0.000 description 1
- KYZUDXYWLHVWCQ-UHFFFAOYSA-N 2-ethenyl-7h-purin-6-amine Chemical group NC1=NC(C=C)=NC2=C1NC=N2 KYZUDXYWLHVWCQ-UHFFFAOYSA-N 0.000 description 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- 241000272194 Ciconiiformes Species 0.000 description 1
- 230000004544 DNA amplification Effects 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 1
- 108060001084 Luciferase Proteins 0.000 description 1
- 239000005089 Luciferase Substances 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 description 1
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 1
- 241000944748 Quesada Species 0.000 description 1
- 239000013614 RNA sample Substances 0.000 description 1
- 102000004167 Ribonuclease P Human genes 0.000 description 1
- 108090000621 Ribonuclease P Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 108091081021 Sense strand Proteins 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 239000008051 TBE buffer Substances 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- DPKHZNPWBDQZCN-UHFFFAOYSA-N acridine orange free base Chemical compound C1=CC(N(C)C)=CC2=NC3=CC(N(C)C)=CC=C3C=C21 DPKHZNPWBDQZCN-UHFFFAOYSA-N 0.000 description 1
- 150000003926 acrylamides Chemical class 0.000 description 1
- 150000001252 acrylic acid derivatives Chemical class 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- DZBUGLKDJFMEHC-UHFFFAOYSA-N benzoquinolinylidene Natural products C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000013626 chemical specie Substances 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- 230000001268 conjugating effect Effects 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 238000007334 copolymerization reaction Methods 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 229920006037 cross link polymer Polymers 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
- 230000001351 cycling effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000000368 destabilizing effect Effects 0.000 description 1
- 230000014670 detection of bacterium Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 208000018459 dissociative disease Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 238000007834 ligase chain reaction Methods 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 230000002101 lytic effect Effects 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012533 medium component Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 238000000329 molecular dynamics simulation Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 108091008104 nucleic acid aptamers Proteins 0.000 description 1
- 102000044158 nucleic acid binding protein Human genes 0.000 description 1
- 108700020942 nucleic acid binding protein Proteins 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 229920002939 poly(N,N-dimethylacrylamides) Polymers 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000012264 purified product Substances 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 125000001424 substituent group Chemical group 0.000 description 1
- 210000004243 sweat Anatomy 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 150000003573 thiols Chemical class 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 235000011178 triphosphate Nutrition 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- 238000007693 zone electrophoresis Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
- G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Electrochemistry (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US7661498P | 1998-03-03 | 1998-03-03 | |
| US60/076,614 | 1998-03-03 | ||
| PCT/US1999/004849 WO1999045374A2 (fr) | 1998-03-03 | 1999-03-03 | Procedes de purification et de detection par electrophorese d'affinite reversible |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2002506204A true JP2002506204A (ja) | 2002-02-26 |
Family
ID=22133151
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000534862A Pending JP2002506204A (ja) | 1998-03-03 | 1999-03-03 | 可逆的アフィニティー電気泳動を用いる精製および検出プロセス |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1068518A2 (fr) |
| JP (1) | JP2002506204A (fr) |
| AU (1) | AU2896399A (fr) |
| CA (1) | CA2322975A1 (fr) |
| WO (1) | WO1999045374A2 (fr) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002350400A (ja) * | 2001-05-29 | 2002-12-04 | Aloka Co Ltd | 電気泳動装置 |
| JP2007512509A (ja) * | 2003-11-05 | 2007-05-17 | エグザクト サイエンシーズ コーポレイション | 反復的親和性分離およびその使用 |
| JP2009516176A (ja) * | 2005-11-10 | 2009-04-16 | テラダイン、 インコーポレイテッド | ローカルに順序付けられたストロービング |
| JP2010508496A (ja) * | 2006-08-29 | 2010-03-18 | ベクトン・ディキンソン・アンド・カンパニー | 無担体の偏向電気泳動の方法及び装置 |
| JP2010536790A (ja) * | 2007-08-17 | 2010-12-02 | パーデュー・リサーチ・ファウンデーション | Psma結合性リガンド−リンカー結合体及び使用方法 |
| US9636413B2 (en) | 2012-11-15 | 2017-05-02 | Endocyte, Inc. | Conjugates for treating diseases caused by PSMA expressing cells |
| US9951324B2 (en) | 2010-02-25 | 2018-04-24 | Purdue Research Foundation | PSMA binding ligand-linker conjugates and methods for using |
| US10188759B2 (en) | 2015-01-07 | 2019-01-29 | Endocyte, Inc. | Conjugates for imaging |
| US10398791B2 (en) | 2013-10-18 | 2019-09-03 | Deutsches Krebsforschungszentrum | Labeled inhibitors of prostate specific membrane antigen (PSMA), their use as imaging agents and pharmaceutical agents for the treatment of prostate cancer |
| US12178892B2 (en) | 2013-11-14 | 2024-12-31 | Purdue Research Foundation | Compounds for positron emission tomography |
| US12208102B2 (en) | 2018-04-17 | 2025-01-28 | Endocyte, Inc. | Methods of treating cancer |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU730491B2 (en) | 1997-05-16 | 2001-03-08 | Exact Sciences Corporation | Electrophoretic analysis of molecules using immobilized probes |
| DE60029092T2 (de) | 1999-04-09 | 2007-02-01 | Exact Sciences Corp., Marlborough | Verfahren zur detektion von nukleinsäuren, welche auf krebs hinweisen |
| US6586177B1 (en) | 1999-09-08 | 2003-07-01 | Exact Sciences Corporation | Methods for disease detection |
| US6849403B1 (en) | 1999-09-08 | 2005-02-01 | Exact Sciences Corporation | Apparatus and method for drug screening |
| FR2799988B1 (fr) | 1999-10-26 | 2001-12-07 | Centre Nat Rech Scient | Procede de separation d'un compose chimique ou biologique dans un melange de composes similaires par diffusion dans un milieu tel qu'un gel |
| US6919174B1 (en) | 1999-12-07 | 2005-07-19 | Exact Sciences Corporation | Methods for disease detection |
| AU4714201A (en) | 1999-12-07 | 2001-06-18 | Exact Sciences Corporation | Supracolonic aerodigestive neoplasm detection |
| FR2806161B1 (fr) * | 2000-03-10 | 2002-09-20 | Sebia Sa | Methode de separation, d'identification et de quantification des isophosphatases alcalines par electrophorese |
| US10337054B2 (en) | 2004-02-02 | 2019-07-02 | Quantum-Si Incorporated | Enrichment of nucleic acid targets |
| US8529744B2 (en) | 2004-02-02 | 2013-09-10 | Boreal Genomics Corp. | Enrichment of nucleic acid targets |
| US9186685B2 (en) | 2012-01-13 | 2015-11-17 | The University Of British Columbia | Multiple arm apparatus and methods for separation of particles |
| WO2005072854A1 (fr) | 2004-02-02 | 2005-08-11 | The University Of British Columbia | Scodaphorese, procedes et appareil utilises pour deplacer et concentrer des particules |
| WO2009094772A1 (fr) | 2008-02-01 | 2009-08-06 | The University Of British Columbia | Procédés et appareil pour l'introduction et la récupération de particules |
| WO2010121381A1 (fr) | 2009-04-21 | 2010-10-28 | The University Of British Columbia | Système et procédés pour la détection de particules |
| CA2742327A1 (fr) | 2011-05-20 | 2012-11-20 | The University Of British Columiba | Systemes et methodes pour amelioration de la scoda (separation, concentration, detection de molecules selon la difference d'affinite de liaison) |
| WO2013166444A2 (fr) | 2012-05-04 | 2013-11-07 | Boreal Genomics Corp. | Analyse de biomarqueurs utilisant la scodaphorèse |
| US9340835B2 (en) | 2013-03-15 | 2016-05-17 | Boreal Genomics Corp. | Method for separating homoduplexed and heteroduplexed nucleic acids |
| US11130986B2 (en) | 2015-05-20 | 2021-09-28 | Quantum-Si Incorporated | Method for isolating target nucleic acid using heteroduplex binding proteins |
| AU2020372908A1 (en) | 2019-10-29 | 2022-06-02 | Quantum-Si Incorporated | Peristaltic pumping of fluids and associated methods, systems, and devices |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5167790A (en) * | 1985-09-27 | 1992-12-01 | Washington University | Field-inversion gel electrophoresis |
| GB9502660D0 (en) * | 1995-02-10 | 1995-03-29 | Univ London | Detection of mutations or variations in DNA |
| US6379515B1 (en) * | 1996-05-24 | 2002-04-30 | Novartis Ag | Process for separating mixtures of substances using capillary affinity gel electrophoresis |
-
1999
- 1999-03-03 EP EP99909853A patent/EP1068518A2/fr not_active Withdrawn
- 1999-03-03 AU AU28963/99A patent/AU2896399A/en not_active Abandoned
- 1999-03-03 CA CA002322975A patent/CA2322975A1/fr not_active Abandoned
- 1999-03-03 WO PCT/US1999/004849 patent/WO1999045374A2/fr not_active Application Discontinuation
- 1999-03-03 JP JP2000534862A patent/JP2002506204A/ja active Pending
Cited By (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002350400A (ja) * | 2001-05-29 | 2002-12-04 | Aloka Co Ltd | 電気泳動装置 |
| JP2007512509A (ja) * | 2003-11-05 | 2007-05-17 | エグザクト サイエンシーズ コーポレイション | 反復的親和性分離およびその使用 |
| JP2009516176A (ja) * | 2005-11-10 | 2009-04-16 | テラダイン、 インコーポレイテッド | ローカルに順序付けられたストロービング |
| US8795494B2 (en) | 2006-08-29 | 2014-08-05 | Becton, Dickinson And Company | Methods and apparatus for carrier-free deflection electrophoresis |
| JP2010508496A (ja) * | 2006-08-29 | 2010-03-18 | ベクトン・ディキンソン・アンド・カンパニー | 無担体の偏向電気泳動の方法及び装置 |
| US10046054B2 (en) | 2007-08-17 | 2018-08-14 | Purdue Research Foundation | PSMA binding ligand-linker conjugates and methods for using |
| US11083710B2 (en) | 2007-08-17 | 2021-08-10 | Purdue Research Foundation | PSMA binding ligand-linker conjugates and methods for using |
| US11717514B2 (en) | 2007-08-17 | 2023-08-08 | Purdue Research Foundation | PSMA binding ligand-linker conjugates and methods for using |
| US11369590B2 (en) | 2007-08-17 | 2022-06-28 | Purdue Research Foundation | PSMA binding ligand-linker conjugates and methods for using |
| JP2010536790A (ja) * | 2007-08-17 | 2010-12-02 | パーデュー・リサーチ・ファウンデーション | Psma結合性リガンド−リンカー結合体及び使用方法 |
| US10646581B2 (en) | 2007-08-17 | 2020-05-12 | Purdue Research Foundation | PSMA binding ligand-linker conjugates and methods for using |
| US11318121B2 (en) | 2007-08-17 | 2022-05-03 | Purdue Research Foundation | PSMA binding ligand-linker conjugates and methods for using |
| US11298341B2 (en) | 2007-08-17 | 2022-04-12 | Purdue Research Foundation | PSMA binding ligand-linker conjugates and methods for using |
| US10406240B2 (en) | 2007-08-17 | 2019-09-10 | Purdue Research Foundation | PSMA binding ligand-linker conjugates and methods for using |
| US8907058B2 (en) | 2007-08-17 | 2014-12-09 | Purdue Research Foundation | PSMA binding ligand-linker conjugates and methods for using |
| US10485878B2 (en) | 2007-08-17 | 2019-11-26 | Purdue Research Foundation | PSMA binding ligand-linker conjugates and methods for using |
| US10517957B2 (en) | 2007-08-17 | 2019-12-31 | Purdue Research Foundation | PSMA binding ligand-linker conjugates and methods for using |
| US10517956B2 (en) | 2007-08-17 | 2019-12-31 | Purdue Research Foundation | PSMA binding ligand-linker conjugates and methods for using |
| US10828282B2 (en) | 2007-08-17 | 2020-11-10 | Purdue Research Foundation | PSMA binding ligand-linker conjugates and methods for using |
| US10624970B2 (en) | 2007-08-17 | 2020-04-21 | Purdue Research Foundation | PSMA binding ligand-linker conjugates and methods for using |
| US10624971B2 (en) | 2007-08-17 | 2020-04-21 | Purdue Research Foundation | PSMA binding ligand-linker conjugates and methods for using |
| US10624969B2 (en) | 2007-08-17 | 2020-04-21 | Purdue Research Foundation | PSMA binding ligand-linker conjugates and methods for using |
| US9951324B2 (en) | 2010-02-25 | 2018-04-24 | Purdue Research Foundation | PSMA binding ligand-linker conjugates and methods for using |
| US12091693B2 (en) | 2010-02-25 | 2024-09-17 | Purdue Research Foundation | PSMA binding ligand-linker conjugates and methods for using |
| US10557128B2 (en) | 2010-02-25 | 2020-02-11 | Purdue Research Foundation | PSMA binding ligand-linker conjugates and methods for using |
| US11155800B2 (en) | 2010-02-25 | 2021-10-26 | Purdue Research Foundation | PSMA binding ligand-linker conjugates and methods for using |
| US9782493B2 (en) | 2012-11-15 | 2017-10-10 | Endocyte, Inc. | Conjugates for treating diseases caused by PSMA expressing cells |
| US10912840B2 (en) | 2012-11-15 | 2021-02-09 | Endocyte, Inc. | Conjugates for treating diseases caused by PSMA expressing cells |
| US9636413B2 (en) | 2012-11-15 | 2017-05-02 | Endocyte, Inc. | Conjugates for treating diseases caused by PSMA expressing cells |
| US11045564B2 (en) | 2013-10-18 | 2021-06-29 | Deutsches Krebsforschungszentrum | Labeled inhibitors of prostate specific membrane antigen (PSMA) as agents for the treatment of prostate cancer |
| US10398791B2 (en) | 2013-10-18 | 2019-09-03 | Deutsches Krebsforschungszentrum | Labeled inhibitors of prostate specific membrane antigen (PSMA), their use as imaging agents and pharmaceutical agents for the treatment of prostate cancer |
| US10471160B2 (en) | 2013-10-18 | 2019-11-12 | Deutsches Krebsforschungszentrum | Labeled inhibitors of prostate specific membrane antigen (PSMA), their use as imaging agents and pharmaceutical agents for the treatment of prostate cancer |
| US11931430B2 (en) | 2013-10-18 | 2024-03-19 | Novartis Ag | Labeled inhibitors of prostate specific membrane antigen (PSMA) as agents for the treatment of prostate cancer |
| US11951190B2 (en) | 2013-10-18 | 2024-04-09 | Novartis Ag | Use of labeled inhibitors of prostate specific membrane antigen (PSMA), as agents for the treatment of prostate cancer |
| US12178892B2 (en) | 2013-11-14 | 2024-12-31 | Purdue Research Foundation | Compounds for positron emission tomography |
| US10188759B2 (en) | 2015-01-07 | 2019-01-29 | Endocyte, Inc. | Conjugates for imaging |
| US10898596B2 (en) | 2015-01-07 | 2021-01-26 | Endocyte, Inc. | Conjugates for imaging |
| US12208102B2 (en) | 2018-04-17 | 2025-01-28 | Endocyte, Inc. | Methods of treating cancer |
Also Published As
| Publication number | Publication date |
|---|---|
| WO1999045374A3 (fr) | 2000-02-17 |
| WO1999045374A2 (fr) | 1999-09-10 |
| EP1068518A2 (fr) | 2001-01-17 |
| AU2896399A (en) | 1999-09-20 |
| CA2322975A1 (fr) | 1999-09-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2002506204A (ja) | 可逆的アフィニティー電気泳動を用いる精製および検出プロセス | |
| US7452668B2 (en) | Electrophoretic analysis of molecules using immobilized probes | |
| US6238927B1 (en) | Reverse displacement assay for detection of nucleic acid sequences | |
| US4302204A (en) | Transfer and detection of nucleic acids | |
| US5824478A (en) | Diagnostic methods and probes | |
| Davison et al. | Purification of Bacillus subtilis RNA polymerase with heparin-agarose. In vitro transcription of phi 29 DNA. | |
| US20020197614A1 (en) | Electrophoretic analysis of target molecules using adapter molecules | |
| US5747254A (en) | Promotion of high specificity molecular assembly | |
| AU760236B2 (en) | Denaturing gradient affinity electrophoresis and methods of use thereof | |
| Brimacombe et al. | [19] Intra-RNA and RNA—protein cross-linking techniques in Escherichia coli ribosomes | |
| US7767468B2 (en) | Repetitive affinity separation and uses therefor | |
| Woodson et al. | Analysis of RNA folding by native polyacrylamide gel electrophoresis | |
| US20090325153A1 (en) | Analysis of heterogeneous nucleic acid samples | |
| AU4053900A (en) | Electrophoretic analysis of target molecules using adapter molecules | |
| JP4940456B2 (ja) | 標的に対して高い親和性で結合する核酸を選択する方法 | |
| Singhal et al. | DNA annealing and DNA–protein interactions by capillary electrophoresis | |
| US20130048487A1 (en) | Systems and methods for enrichment and detection of particles | |
| WO2013026161A1 (fr) | Systèmes et procédés pour l'enrichissement et la détection de particules | |
| JP4019433B2 (ja) | 核酸結合用粒子担体 | |
| JP2003532073A5 (fr) | ||
| Expert-Bezançon et al. | [20] RNA—protein cross-linking | |
| JP2006166785A (ja) | 相互作用測定方法 | |
| CA2750428A1 (fr) | Systemes et methodes pour l'enrichissement et la detection de particules |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20060303 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20090313 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20090903 |