JP2007070263A - Composition for preventing diabetes mellitus - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a composition for preventing diabetes mellitus, derived from buckwheat extremely effective for prophylaxis/relaxation of diabetes mellitus and prevention of obesity and to provide a method for producing the composition. <P>SOLUTION: The composition for prophylaxis of diabetes mellitus comprises rutin as an active ingredient. The composition for prophylaxis of diabetes mellitus comprises a solvent extract of the terrestrial part containing at least one selected from the leaf part, the stem part, the flower part and seed of buckwheat. The extracting solvent of the solvent extract is preferably water-containing ethanol. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a composition for preventing diabetes.

There are two types of diabetes, insulin-dependent diabetes (type I diabetes) and non-insulin-dependent diabetes (type II diabetes), of which 90% are of the latter type.
The latter (type II diabetes) is said to be caused by abnormal sugar / lipid metabolism due to insufficient insulin action due to overnutrition (eg, decreased insulin sensitivity in tissues, insufficient insulin secretion in pancreas, etc.).
Non-insulin-dependent diabetes mellitus (type II diabetes) is basically controlled by diet therapy and exercise therapy rather than drug treatment, but even if such self-management is carried out, metabolic abnormalities are not necessarily corrected. It is not always true, and it tends to depend on drugs.
However, there are problems with drugs in terms of side effects, dosages, and restrictions on use. Moreover, since these are mostly due to the mixture of the unified substances, there are problems from the side effects of the single substance and the safety caused by long-term use.

  Conventionally, extracts derived from leaves such as Gymnema, guava, banaba and mulberry are known as plant-derived substances that correct such metabolic abnormalities of carbohydrates. Since these plant-derived extracts are easy to apply to foods, pharmaceuticals and the like from the viewpoint of safety, research on active ingredients and the like has been conducted.

Under such a background, the present inventors came to pay attention to buckwheat ethanol extract for extracts derived from various plants.
As a result of various experiments, it was found that rutin contained in this extract effectively lowers the blood glucose level.
It is an object of the present invention to provide a composition for preventing diabetes derived from buckwheat that is extremely effective in preventing and / or alleviating diabetes and preventing obesity, and a method for producing them.

In order to solve the above problems, the composition for preventing diabetes according to the present invention is characterized by containing rutin as an active ingredient.
In addition, the composition for preventing diabetes according to the present invention is characterized by containing an above-ground solvent extract containing at least one of leaves, stems, flowers and seeds of buckwheat (buckwheat).
Furthermore, it is preferable that the extraction solvent of the solvent extract is hydrous ethanol.

BEST MODE FOR CARRYING OUT THE INVENTION

  Hereinafter, embodiments of the present invention will be described in detail.

  The composition for preventing diabetes of the present invention comprises rutin as an active ingredient. Rutin is a kind of flavonol glycoside represented by the following chemical formula.

The raw material for extracting rutin is not particularly limited. It is preferable to obtain from soba.
Buckwheat (soba) is an annual plant of the Tedaceae family, and its seed is known as a raw material for “buckwheat flour”. Triangular leaves alternate with each other and have a Saya-shaped bamboo leaf surrounding the stem at the base of the petiole. The stem has a cylindrical shape with a depression on one side, and a large number of flowers are attached at the tip of the stem with a general inflorescence.

  As a raw material for extracting the composition for preventing diabetes of the present invention, an above-ground part containing at least one of buckwheat leaf part, stem part, flower part and seed is mainly used. That is, a mixture of leaves, stems, flowers, and seeds may be used, or any of leaves, stems, flowers, and seeds may be used alone. Moreover, you may use what mixed the root part etc. as an extraction raw material. Of these, those containing leaves are particularly preferred. This is because the content of active ingredients such as rutin can be further increased.

The buckwheat varieties are divided into summer buckwheat, autumn buckwheat, or an intermediate type between the two depending on the cropping season. tataricum Gaertn. (Also referred to as “Nigasoba”), perennial Shakuchirisoba: F. cymosum Meisn. (It is also referred to as Sukkon Soba.)
As a raw material for extracting the composition for preventing diabetes according to the present invention, this species is preferably used, but any of the above varieties can be used. Also, the production area is not limited.

Extract raw materials such as buckwheat leaf part, stem part, flower part, seed and the like can be used as they are, but a dry product is preferably used. As a drying method, it can be dried by a publicly known method such as shade drying, air drying, hot air drying, freeze drying and the like.
The extraction raw material may be a pulverized product cut or pulverized into a size of several mm or less by a pulverizer or the like. Use of such a pulverized product facilitates extraction of active ingredients.

  As a solvent for extracting the composition for preventing diabetes according to the present invention, a solvent such as water, methanol, ethanol, isopropyl alcohol, acetone, 1,3-butylene glycol, ethylene glycol, propylene glycol, glycerin, ethyl acetate or the like is used. Good. A mixture of two or more of these solvents can also be used. Preferably, when water or hydrous ethanol is used as the extraction solvent, the active ingredient is efficiently extracted. In particular, hydrous ethanol is an extraction solvent that is easy to maintain the activity of the active ingredient during extraction and is preferable from the viewpoint of safety of the extract in food use.

  When water is used as the extraction solvent, the extraction temperature is 20 to 100 ° C., preferably about 80 to 100 ° C. This is because if the extraction temperature is too low, it is difficult to extract active ingredients. The kind of water for extraction is not particularly limited, and tap water, distilled water, mineral water, alkaline ionized water and the like can be used. Preferably, deep water (deep ocean water) is used as the extraction solvent.

  When hydrous ethanol is used as the extraction solvent, the ethanol concentration is preferably 30 to 90% (volume ratio). This is because if the amount is less than about 30% (volume ratio) or exceeds 90% (volume ratio), the extraction amount of the active ingredient tends to decrease. The extraction temperature is 20 to 80 ° C, preferably about 50 to 80 ° C. In addition, the water-containing ethanol extraction may be repeated at various concentrations in order to improve the content of the active ingredient.

  As a method for extracting the composition for preventing diabetes according to the present invention, any method such as continuous extraction, immersion extraction, countercurrent extraction, and supercritical extraction can be employed, and an arbitrary apparatus is used at room temperature or under reflux heating. be able to.

As a specific method, an extraction raw material is put into a treatment tank filled with an extraction solvent, and an active ingredient is eluted while stirring. For example, when water or water-containing ethanol is used as the extraction solvent, the extraction is performed for about 30 minutes to 2 hours using a polar solvent of about 5 to 100 times (weight ratio) of the extraction raw material. The active ingredient is eluted in the solvent, and then filtered to remove the extraction residue to obtain an extract. Thereafter, the extract is subjected to treatments such as dilution, concentration, drying and purification according to a conventional method to obtain a composition for preventing diabetes.
Examples of the purification method include activated carbon treatment, resin adsorption treatment, ion exchange resin, liquid-liquid countercurrent distribution, and the like, but they are not used in large quantities when added to foods. It may be used unpurified.

  The composition for diabetes prevention of this invention can be contained in food-drinks. For example, confectionery (gum, candy, caramel, chocolate, cookies, snacks, jelly, gummi, tablet confectionery, etc.), noodles (soba, udon, ramen, etc.), dairy products (milk, ice cream, yogurt, etc.), seasonings (Miso, soy sauce, etc.), soups, beverages (juice, coffee, tea, tea, carbonated drinks, sports drinks, etc.), general foods, health foods (tablets, capsules, etc.), nutritional supplements (nutrient drinks) Etc.).

You may add the composition for diabetes prevention of this invention to an instant foodstuff. For example, a composition obtained by spray-drying or freeze-drying a composition for preventing diabetes together with powdered cellulose can be easily contained in a food or drink by making it into a powder, granule, tablet or solution.
In addition, the composition for preventing diabetes according to the present invention can be dissolved in, for example, fats and oils, ethanol, propylene glycol, glycerin or a mixture thereof to be liquefied and added to beverages or added to solid foods. Further, if necessary, it can be mixed with a binder such as gum arabic or dextrin to form a powder or granule and added to a beverage or a solid food.

When the composition for preventing diabetes according to the present invention is applied to foods and drinks, since the purpose is to prevent diseases and maintain health, the content of the composition for preventing diabetes is 1 to 20 weights in total for foods and drinks. % Is preferred.

You may use the composition for diabetes prevention of this invention as an active ingredient of a pharmaceutical. The “medicament” of the present invention may be either a medicinal product or a quasi-drug as long as it is used for prevention or treatment of diseases.
The method for administering the composition for preventing diabetes as a pharmaceutical is preferably administered orally. Generally, it can be in the form of tablets, pills, soft / hard capsules, fine granules, powders, granules, liquids and the like.
Moreover, you may administer the pharmaceutical of this invention parenterally. When administered as a parenteral preparation, it can be in the form of an injection, a suppository, or the like in the form of a solution or with a dispersion, suspension, stabilizer or the like added.

The dosage of the composition for preventing diabetes varies depending on age, body weight, symptoms, therapeutic effect, administration method, treatment time, etc., but usually ranges from 1 mg to 1000 mg per adult and once to several times a day. It is administered orally once, or parenterally once to several times a day in the range of 100 μg to 100 mg at a time.
In addition, since the dose varies depending on various conditions, a dose smaller than the above dose may be sufficient, or it may be necessary to administer beyond the range.

Hereinafter, embodiments embodying the present invention will be described in detail.
A dried product comprising a buckwheat leaf portion and a stem portion (including a small amount of flower portion) was obtained and pulverized by a pulverizer. 5 kg of this pulverized product was used as a raw material for extraction.
About 10 times the amount of hydrous ethanol having an ethanol concentration of 90% (volume ratio) was added to the pulverized raw material, and extraction was performed by heating under reflux for 2 hours. Thereafter, the aqueous ethanol extract was filtered to remove the residue, and the solvent of the filtrate was removed to obtain a buckwheat extract (buckwheat extract 400 g).

  In order to examine the functionality of the produced buckwheat extract, the following animal experiment was conducted.

  Rats were fed a diet containing buckwheat extract produced by the above method. For comparison, rats were fed a diet that did not contain buckwheat leaf extract.

  Male Otsuka Long-Evans Tokushima Fatty rats (hereinafter referred to as OLETF rats) that genetically spontaneously develop one-month-old type 2 diabetes, and male Long-Evans Tokushima Otsuka rats (hereinafter referred to as diabetes) that do not develop diabetes in the control model animals. LETO rat). Rats were housed in an animal breeding room with a light cycle of room temperature 22 ± 1 ° C., room temperature 55 ± 5%, 8: 00 &#12316; 20: 00.

  During the first 3 months, MF chow diets were given to LETO and OLETF rats for preliminary breeding. Since OLETF rats usually develop type 2 diabetes from 5 to 8 months of age, we started eating the test meal together with LETO rats from 4 months of age one month before the possibility of onset.

  After 4 hours of fasting for 6 hours (9:00 &#12316; 15:00), blood was collected from the tail vein, blood glucose level was measured, and grouped into 6 animals per group so that body weight and blood glucose level were equal Then, the following test meals were fed freely for 5 months.

  The test diet was a purified diet based on AIN-76, which was fed to a control group of LETO and OLETF rats. Weight composition (g / kg) of feed is 200 casein, 100 corn oil, 35 mineral mixture (AIN-76-MX), 35 vitamin mixture (AIN-76-VX), 50 cellulose, 2 choline bitartrate, DL- Methionine 3, corn starch 150 and sucrose 450 were used. In the buckwheat leaf extract-added food, buckwheat extract was added 5% of the total weight of the feed, and the amount of sucrose was reduced by the added amount. During the breeding period, food intake was measured every day, and body weight and water intake were measured every other day.

  1, 2, 3, 4 and 5 months after the start of test meal administration, blood glucose was measured using a Medisafe chip (Terumo, Tokyo) and blood was collected from the tail vein after fasting for 6 hours. FIG. 1 shows the change in blood glucose level in rats over 5 months after taking the test meal.

  As shown in FIG. 1, the blood glucose level of LETO rats that did not develop diabetes who took the control diet was low throughout the breeding period. On the other hand, the blood glucose level of OLETF rats that developed diabetes that took the control diet increased over time, and was considered to have developed diabetes. The blood glucose level of OLETF rats fed with buckwheat extract did not rise significantly during the breeding period, and remained at a significantly lower level than that of OLETF rats fed control food. From this, it was revealed that buckwheat extract has an excellent inhibitory effect on the increase in blood sugar.

  Serum insulin concentrations in OLTF rats fed control diets decreased 5 months after the onset of diabetes, but insulin concentrations in OLETF rats fed buckwheat extract showed the same high level as LETO rats. The results are shown in Table 1. It was observed that buckwheat extract inhibits the decrease in insulin secretion by inhibiting the functional damage of pancreatic islets of Langerhans due to the onset of diabetes.

  Serum and liver triglyceride levels in OLETF rats fed the control diet were markedly elevated in LETO rats, but buckwheat extract effectively reduced serum and liver triglyceride concentrations in OLETF rats. The results are shown in Table 2.

Buckwheat extract reduced liver cholesterol levels. The results are shown in Table 3.

  From the above results, it was revealed that buckwheat extract effectively suppresses the onset of diabetes. It was also suggested that reducing lipid concentration has an effect of improving lipid metabolism in animals genetically predisposed to type 2 diabetes.

The following is a blending example of buckwheat extract used as a composition for preventing diabetes. The following formulation examples do not limit the gist of the invention and are not limited to the following formulation examples as long as they are effective as a composition for preventing diabetes.
Formulation Example 1: Chewing gum
53.0% by weight sugar
Gum base 20.0
Glucose 10.0
Minamata 16.0
Fragrance 0.5
Buckwheat extract 0.5
100.0% by weight

Formulation Example 2: Gummy
Reduced water tank 40.0% by weight
Granulated sugar 20.0
Glucose 20.0
Gelatin 4.7
Water 9.68
Ume Juice 4.0
Ume Flavor 0.6
Dye 0.02
Buckwheat extract 1.0
100.0% by weight

Formulation Example 3: Candy
50.0% by weight sugar
Minamata 33.0
Water 14.4
Organic acid 2.0
Fragrance 0.2
Buckwheat extract 0.4
100.0% by weight

Formulation Example 4: Yogurt (hard / soft)
41.5% by weight of milk
Nonfat dry milk 5.8
Sugar 8.0
Agar 0.15
Gelatin 0.1
Lactic acid bacteria 0.005
Buckwheat extract 0.4
Perfume
Water residue
100.0% by weight

Formulation Example 5: Soft capsule
Brown rice germ oil 87.0% by weight
Emulsifier 12.0
Buckwheat extract 1.0
100.0% by weight

Formulation Example 6: Coffee beverage (liquid)
Roasted coffee beans 6.0% by weight
Sugar 6.0
Baking soda 0.2
Emulsifier 0.15
Buckwheat extract 1.0
Water residue
100.0% by weight

Formulation Example 7: Coffee drink (powder)
Instant coffee 90.0% by weight
Skim milk 7.0
Buckwheat extract 3.0
100.0% by weight

Formulation Example 8: Soft drink
Fructose glucose liquid sugar 30.0% by weight
Emulsifier 0.5
Buckwheat extract 0.05
Perfume
Purified water residue
100.0% by weight

Formulation Example 9: Tablet
Lactose 54.0% by weight
Crystalline cellulose 30.0
Starch degradation product 10.0
Glycerin fatty acid ester 5.0
Buckwheat extract 1.0
100.0% by weight

Formulation Example 10: Tablet
76.4% by weight of sugar
Glucose 19.0
Sucrose fatty acid ester 0.2
Buckwheat extract 0.5
Purified water 3.9
100.0% by weight

  As described above, buckwheat extract can be sufficiently used as a food and drink or a medicine having an effect on diabetes prevention and improvement.

It is a graph which shows the change of the rat blood glucose level for 5 months which ingested the test meal in a present Example.

Claims (5)

  A composition for preventing diabetes comprising rutin as an active ingredient.   A composition for preventing diabetes, comprising an above-ground solvent extract containing at least one of leaves, stems, flowers, and seeds of buckwheat (buckwheat).   The composition for diabetes prevention of Claim 1 or 2 whose extraction solvent of the said solvent extract is a hydrous ethanol.   A pharmaceutical comprising the composition for preventing diabetes according to any one of claims 1 to 3.   Food / beverage products containing the diabetes prevention composition of any one of Claims 1-3.

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