JP2007170979A - Feces sampling container - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a feces sampling container capable of keeping the internal pressure of a container body by leaking air from the point of time when a feces sampling rod is inserted into the container body. <P>SOLUTION: This feces sampling container is constituted of the container body comprising a cylindrical feces sampling tube wherein a dissolving liquid storage chamber for storing dissolving liquid for dissolving feces is provided inside, and a filtrate tube comprising a cylindrical container mounted on one end of the feces sampling tube; and the feces sampling rod 30 wherein the base end side of a rod-shaped member is used as a gripping part 37 and a feces sampling part 31 for sampling feces is provided on the tip side. The feces sampling part 31 of the feces sampling rod 30 is constituted of a tip part 32 comprising a quadrangular pyramid having the vertex on the axis and a shaft part 33 comprising a quadrangular prism, and the quadrangular pyramid and the quadrangular prism form a continuous ridgeline. Each long recessed part 33a is formed in the axial direction respectively on facing surfaces on an upper half part and on a lower half part of the shaft part 33, and the inside of each recessed part 33a is partitioned by a rib 33b disposed in the axial direction. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a stool collection container used when examining stool as a specimen.

  Conventionally, as a stool collection container used in the fecal occult blood test and various virus tests, for example, the one shown in Patent Document 1 is known. This stool collection container has a container body whose both ends are open and closed with a thin film, a cap member which closes one end of the container body, and a sample collection means (stool collection rod) which is inserted into the other end of the container body. And a solution for dissolving feces is stored in the solution storage chamber between the cap member and the thin film. When the sample collection means is inserted from the open end of the container main body, excess stool adhering to the sample collection unit is wiped off with a thin film jar, and only a certain amount of stool is supplied to the solution storage chamber. During the inspection, the cap member's suspension is filtered by a filter by twisting the closure at the tip of the cap member by hand, pressing the center of the container body with the hand and pressing the inside. It is discharged.

However, in the case of a conventional stool collection container represented by Patent Document 1, when the sample collection means is inserted into the solution storage chamber, the internal pressure of the solution storage chamber rises and the solution leaks down through the filter. The inspection may be hindered.
Therefore, in Patent Document 2, a protruding wall that is provided inside the container body and that is airtightly engaged with the outer peripheral surface of the stool collection rod is surrounded by a separation wall that removes excess feces when the stool collection rod is inserted. In addition, a stool collection container is disclosed in which a recess that is longer than the axial length of the ridge of the separation wall is formed on at least a part of the peripheral surface of the stool collection rod so as to face the separation wall. According to the stool collection container described in Patent Document 2, the internal pressure of the container body, which has been raised by inserting the stool collection bar into the container body, can be reduced to normal pressure immediately before the completion of the insertion process. When the part is punctured, the suspension does not erupt from the container main body due to excessive pressure and contaminate the surroundings.
JP-A-8-292189 Japanese Patent No. 2668815

  However, even in the stool collection container described in Patent Document 2, the internal pressure of the container body rises until just before the completion of the insertion process, and during that time, the solution may pass through the filter and leak out of the container body. There is.

  On the other hand, in the pretreatment process of preparing the centrifugal supernatant of the stool specimen suspension, not only the droplet infection due to aerosol, but also contact infection accidents due to contamination such as droplet leakage or adhesion during inadvertent stool suspension work. Because of the possibility of happening, all stool suspension operations are carried out in the safety cabinet in the P3 laboratory and are forced to be inefficient.

  The present invention has been made in view of the above-described problems, and an object thereof is to provide a stool collection container in which the internal pressure of the container body is maintained at normal pressure by air leaking from the time when the stool collection rod is inserted into the container body. And

In order to achieve the above object, the stool collection container according to the present invention is provided with a solution storage chamber for storing a solution for dissolving feces, and one end of the solution storage chamber is sealed with a breakable membrane. A tubular stool collection tube that is stopped, and a stool collection rod provided with a stool collection part that collects feces on the distal end side of the rod-like member as a gripping part, and the stool collection part includes a shaft It is characterized by comprising a tip portion made of a pyramid having a top on a line and an axial portion made of a prism, and the ridge line in which the pyramid and the prism are continuous.
The conventional stool collection portion of the stool collection rod has a conical tip and a cylindrical column, so that when the membrane for sealing the solution storage chamber is broken through the stool collection membrane, There was variation in the direction. In contrast, in the present invention, since the tip of the stool collection portion has a pyramid shape and the shaft portion has a prism shape, the membrane can be sharply broken at the ridge line portion of the pyramid and the prism, and the membrane is broken. There is no constant variation. Then, air flows from the gap formed between the broken and triangular film piece and the prismatic shaft portion, and the internal pressure of the solution storage chamber starts when the stool collection rod is inserted into the solution storage chamber. Is kept at normal pressure.

  According to the present invention, the shape of the stool collection portion of the stool collection rod has a pyramid shape at the tip and a prismatic shape at the shaft portion. Therefore, when the membrane that seals the solution storage chamber is pierced by the stool collection portion However, there is no constant variation in how the film breaks. Then, air flows from the gap formed between the fractured membrane and the prismatic shaft, and the internal pressure of the solution storage chamber can be maintained at normal pressure from the time when the stool collection rod is inserted into the solution storage chamber. it can.

  In the stool collection container according to the present invention, the shape of the stool collection portion of the stool collection rod is formed between the broken membrane and the prismatic shaft portion by making the tip portion into a pyramid shape and the shaft portion into a prism shape. The air is circulated from the gap generated in the above, and the internal pressure of the solution storage chamber is maintained at normal pressure from the time when the stool collection rod is inserted into the solution storage chamber. Preferably it is.

In the stool collection container according to the present invention, it is preferable that a concave portion for accommodating feces is formed in the shaft portion of the stool collection portion, and a rib for partitioning the concave portion is provided in the concave portion.
When the membrane that seals the solution storage chamber is pierced by the stool collection rod, the broken membrane piece may adhere to the stool collection rod. In particular, if the stool collection part is provided with a recessed part that accommodates feces, the torn film piece enters the recessed part and the air flow passage is blocked, not only the internal pressure of the solution storage chamber increases, but also the tear is broken. There is also a possibility that the film piece scrapes off the stool in the recess. On the other hand, in the present invention, by providing a rib for partitioning the recessed portion in the recessed portion, the torn film piece is prevented from entering the recessed portion to ensure an air flow path, and in the recessed portion. Prevent feces from being scraped off.

Moreover, in the stool collection container which concerns on this invention, the both sides of the axial part of a stool collection part may protrude in the opening side of a recessed part.
In the present invention, since both side portions of the shaft portion protrude toward the opening side of the recessed portion, it is possible to ensure a gap serving as an air flow path on the upper surface of the recessed portion.

Further, in the stool collection container according to the present invention, a cylindrical sealing member is inserted into one end portion of the solution storage chamber, and a breakable membrane is stretched on one end surface of the sealing member. A convex seal portion may be provided around the outer peripheral portion, and a plurality of fins may be provided radially above the seal portion.
In the present invention, extra stool adhering to the stool collection rod is accommodated in the outer peripheral portion of the sealing member (between the fins), and a convex seal portion is provided around the outer peripheral portion of the sealing member, The excess feces stored is prevented from entering the solution storage chamber from the outer periphery of the sealing member.

Embodiments of a stool collection container according to the present invention will be described below with reference to the drawings.
The stool collection container in this embodiment is attached to a tubular stool collection tube 10 in which a solution storage chamber 11 for storing a solution for dissolving stool is provided, and to one end of the stool collection tube 10. A container body 50 comprising a filtrate tube 20 comprising a cylindrical container, and a stool collection rod 30 provided with a stool collection part 31 for collecting stool at the distal end side, with the proximal end side of the rod-shaped member being a gripping part 37 It is composed of The shape of the container body 50 is shown in FIG. 1, and the shape of the stool collection rod 30 is shown in FIGS. In the following description, the direction in which the stool collection rod 30 is inserted into the stool collection tube 10 will be referred to as “down” and the opposite direction as “up” for convenience.

The solution storage chamber 11 is provided in an intermediate portion of the stool collection tube 10, and a buffer solution is stored in the solution storage chamber 11 as a solution. Here, the solution is sterilized distilled water, physiological saline, phosphate buffer, or the like.
While the upper end of the solution storage chamber 11 is sealed with a stocker 12 (sealing member), the lower end of the solution storage chamber 11 is partitioned by a filter 13 that filters the suspension, and in a state where no load is applied, The solution is prevented from leaking from the solution storage chamber 11 through the filter 13.
Further, a male screw portion 15 is formed on the outer peripheral surface of the lower end portion of the stool collection tube 10 adjacent to the filter 13, and is screwed into a female screw portion 21 formed on the inner peripheral surface of the filtrate tube 20. Yes. On the other hand, an internal thread portion 14 is formed on the inner peripheral surface of the upper end portion of the stool collection tube 10 adjacent to the stocker 12, and an external thread portion 36 of the stool collection rod 30 to be described later is screwed together.

The stocker 12 is formed in a cylindrical bottom portion 12d inscribed in the inner peripheral surface of the stool collection tube 10, a cylindrical portion 12b having a smaller diameter than the bottom portion 12d, and radially formed on the outer peripheral surface of the cylindrical portion 12b, and is longer than the cylindrical portion 12b. The plurality of fins 12c are integrally formed. A breakable film 12a made of polyethylene, elastomer, aluminum or the like is stretched on the upper end surface of the cylindrical portion 12b, and a convex seal portion 12e is provided on the outer peripheral surface of the bottom portion 12d.
Excess stool adhering to the stool collection rod 30 is wiped off by the torn membrane 12a and the plurality of fins 12c, and accumulates on the stocker 12. However, when the stool collection container is subjected to a centrifuge, these excess stool is collected. Moves between the fins 12c. At this time, since the seal portion 12e is provided around the outer peripheral surface of the bottom portion 12d, the excess stool stored does not enter the solution storage chamber 11 from the outer periphery of the stocker 12.

The stool collection portion 31 of the stool collection rod 30 is composed of, for example, a tip portion 32 made of a quadrangular pyramid and a shaft portion 33 made of a quadrangular prism having apexes on the axis, and the quadrangular pyramid and the quadrangular prism form a continuous ridgeline. is doing. In the case of the present stool collection rod 30, the film 12a of the stocker 12 is broken in a cross shape at the ridge line portion of the stool collection part 31, and the way the film 12a is broken does not vary. Then, the air is circulated through the gap formed between the broken and triangular film piece and the shaft portion 33 made of a quadrangular column, and the solution is taken from the time when the stool collection rod 30 is inserted into the solution storage chamber 11. The internal pressure of the storage chamber 11 is kept at normal pressure.
In the upper half portion and the lower half portion of the shaft portion 33, concave portions 33a that are alternately long in the axial direction are formed on the opposing surfaces, respectively, and feces are accommodated in the respective recessed portions 33a.
The stool collection part 31 has a shape suitable for being scraped while being pressed against the stool and accommodated in the recessed part 33a without scooping the stool, and prevents the collection of stool of a specified amount or more in advance. However, it is possible to guide the feces not to be collected in a heap like the conventional sagittal stool collection part.

Each recess 33a is partitioned in two rows by axially arranged ribs 33b to prevent the torn film 12a from entering the recess 33a and secure an air flow path. The feces in the recess 33a is prevented from being scraped off. In addition, as shown in FIG. 3, both side portions 33c and 33c disposed in the axial direction with the rib 33b interposed therebetween protrude to the opening side of the recessed portion 33a, and serve as an air flow path on the upper surface of the recessed portion 33a. A void can be secured.
In addition, the concave portion 33a is partitioned by the rib 33b into a slit shape, and the width of the concave portion 33a is moderately narrow. Therefore, when collecting watery stool, the surface tension of the liquid is used. The stool collection rod 30 can be taken up in a state where the recessed portion 33a is filled with watery stool.

  Between the stool collection portion 31 and the gripping portion 37, a columnar reduced diameter portion 34, a columnar enlarged diameter portion 35, and a male screw portion 36 are sequentially formed from the stool collection portion 31 toward the gripping portion 37. . The reduced diameter portion 34 has a smaller diameter than the stool collection portion 31 in order to secure an air flow passage. On the other hand, the distal end side of the enlarged diameter portion 35 is tapered, and after the film 12a of the stocker 12 is broken, the enlarged diameter portion 35 closes the opening of the stocker 12 so that feces do not leak out.

The stool collection tube 10 is preferably formed of, for example, polypropylene so that it can withstand cryopreservation and centrifugation. Moreover, in order to improve the adhesiveness of the stool collection tube 10 and the filtrate tube 20, it is suitable to use low density polyethylene etc. which changed the density for the filtrate tube 20. The stocker 12 is preferably formed of low density polyethylene, and the stool collection rod 30 is preferably formed of ABS resin or the like.
On the other hand, the filter 13 is preferably a sintered body made of a synthetic resin such as polypropylene or polyethylene, but since the size of the detection target varies, the pore diameter of the filter 13 may be appropriately selected depending on the detection target.

Next, the handling of the stool collection container in the sorting operation of the detection object will be described.
Before the stool collection, the empty filtrate tube 20 is attached to the lower end of the stool collection tube 10 packed with the buffer solution, and the stool collection rod 30 is removed in advance. When collecting the stool using the stool collection rod 30, the stool collection rod 30 to which the stool is attached is inserted from the upper end opening of the stool collection tube 10, and the membrane 12a of the stocker 12 is inserted at the tip 32 of the stool collection unit 31. Then, the stool collection portion 31 is inserted into the solution storage chamber 11, and the male thread portion 36 of the stool collection rod 30 is screwed into the female thread portion 14 of the stool collection tube 10 to be sealed (see FIG. 4).

  It should be noted that the screw between the stool collection tube 10 and the stool collection rod 30 (of the stool collection tube 10) immediately before being placed on the centrifuge so that the suspension smoothly moves from the stool collection tube 10 to the filtrate tube 20. Between the female threaded portion 14 and the male threaded portion 36 of the stool collection rod 30) and between the stool collection tube 10 and the filtrate tube 20 (between the male threaded portion 15 of the stool collection tube 10 and the female threaded portion 21 of the filtrate tube 20). The airtightness may be released by loosening. Since the male thread portion 15 of the stool collection tube 10 is screwed deeply into the filtrate tube 20, the suspension that has passed through the filter 13 does not leak out of the filtrate tube 20.

  The filtrate tube 20 is preferably designed so that it can be removed from the stool collection tube 10 and transferred directly to the next step after centrifugation. For example, in this embodiment, the outer shape of the filtrate tube 20 conforms to the standard size of a 96-well microtiter plate of 8 rows and 12 columns. Match the pitch of the well. In the next step, a necessary amount of the suspended liquid of the detection target can be collected using a standard 8-series micropipette.

Next, another embodiment of the stool collection container according to the present invention will be described.
In this embodiment, a filtrate tube is not used, and a stool collection tube and a stool collection rod are used. The stool collection rod has the same shape as in the previous embodiment, and the stool collection tube is different from that in the previous embodiment. In FIG. 5, the longitudinal cross-sectional view of the stool collection tube in a present Example is shown.

  In the stool collection tube 40 in this embodiment, the upper end portion of the solution storage chamber 41 is sealed with the stocker 12, and the female thread portion 44 is formed on the inner peripheral surface of the upper end portion of the stool collection tube 40. Although it is the same as the stool collection tube 10 in the example, the solution storage chamber 41 has a bottom portion 43, there is no filter for filtering the suspension, and a male screw portion is formed on the outer peripheral surface of the lower end portion of the stool collection tube 40. It is different in that it is not.

  When inserting the stool collection rod 30 into the solution storage chamber 41, as in the previous embodiment, the membrane piece 12 having a triangular shape by breaking the membrane 12 a of the stocker 12 into a cross shape at the ridge line portion of the stool collection portion 31. And air flows through a gap formed between the shaft portion 33 made of a rectangular column. Therefore, the internal pressure of the solution storage chamber 41 is maintained at a normal pressure from the time when the stool collection rod 30 is inserted into the solution storage chamber 41.

  When collecting the suspension in the solution storage chamber 41 after stool collection, the needle 43 of a syringe (not shown) may be stabbed into the bottom 43 of the stool collection tube 40 and sucked, or the stool collection tube 40 may be aspirated. After removing the stool collection rod 30, it may be sucked with a spoid or the like.

  As mentioned above, although the Example of this invention was described, this invention is not limited to said Example, In the range which does not deviate from the meaning, it can change suitably. For example, in the above embodiment, the stool collection portion has a quadrangular pyramid at the tip and a quadrangular prism at the tip, but may have a triangular pyramid at the tip and a triangular prism. Further, in the above-described embodiment, the recessed portions are provided alternately on the opposing surfaces of the stool collection unit, but may be provided on adjacent surfaces or on the same surface side. The point is that the desired function can be obtained in the present invention.

It is a longitudinal cross-sectional view of the container main body in one Example of the stool collection container which concerns on this invention. It is a side view of a stool collection stick in one example of a stool collection container concerning the present invention. (A) A direction arrow directional view in FIG. 2, (b) It is a BB arrow directional cross-sectional view in FIG. In one Example of the stool collection container which concerns on this invention, it is a longitudinal cross-sectional view which shows the state which inserted the stool collection stick | rod in the container main body. It is a longitudinal cross-sectional view of the stool collection tube in the other Example of the stool collection container based on this invention.

Explanation of symbols

10, 40 Stool collection tubes 11, 41 Dissolution chamber 12 Stocker (sealing member)
12a Membrane 12b Cylindrical part 12c Fin 12d Bottom part 12e Seal part 13 Filter 14, 21, 44 Female thread part 15, 36 Male thread part 20 Filtrate tube 30 Stool collection rod 31 Stool collection part 32 Tip part 33 Shaft part 33a Recessed part 33b Rib 33c Both sides Part 50 Container body

Claims (4)

A stool storage chamber for storing a lysis solution for dissolving stool is provided therein, and a tubular stool collection tube in which one end of the lysis solution storage chamber is sealed with a breakable film, and a base end of a rod-shaped member The side is a gripping part, and provided with a stool collection rod provided with a stool collection part for collecting feces on the tip side,
The said stool collection part consists of the front-end | tip part which consists of a pyramid which has a top part on an axis line, and the axial part which consists of prisms, The ridgeline which the said pyramid and the said prisms form is formed, The stool collection container characterized by the above-mentioned.   2. The stool collection container according to claim 1, wherein a concave portion that accommodates feces is formed in a shaft portion of the stool collection portion, and a rib that divides the concave portion is provided in the concave portion.   The stool collection container according to claim 2, wherein both side portions of the shaft portion of the stool collection portion protrude toward the opening side of the recessed portion. A cylindrical sealing member is inserted into one end of the solution storage chamber,
The breakable film is stretched on one end surface of the sealing member, a convex seal portion is provided around the outer peripheral portion of the sealing member, and a plurality of fins are arranged radially above the seal portion. The stool collection container according to any one of claims 1 to 3, wherein the stool collection container is disposed on the stool.

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