JP2007195411A - Liver dysfunction restraining agent - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a liver dysfunction restraining agent, and to provide food and drink for restraining liver dysfuction. <P>SOLUTION: This liver dysfunction restraining agent has as the active ingredients, components contained in cheese. The food and drink for restraining liver dysfuction are mixed with components contained in cheese. The components contained in cheese have action of restraining liver dysfunction and remarkably improve liver function. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

    The present invention relates to a liver dysfunction inhibitor containing as an active ingredient a component contained in cheese, or a food or drink for inhibiting liver dysfunction containing a component contained in cheese. Since the liver dysfunction inhibitor and the food and drink for suppressing liver dysfunction according to the present invention can suppress liver dysfunction such as alcoholic liver dysfunction by ingesting this, diseases caused by liver dysfunction Useful for treatment and prevention.

    The liver is an important organ having various functions such as metabolism / storage of carbohydrates, proteins, lipids, etc., and decomposition / detoxification of harmful substances. Such liver function is impaired by various factors such as viral infection, stress, smoking, unhealthy eating habits, alcohol consumption, etc., resulting in acute hepatitis, chronic hepatitis, viral hepatitis, alcoholic fatty liver , The risk of diseases such as cirrhosis and liver cancer is increased. In particular, when the intake of alcohol increases in the dietary life, the risk that liver function will be impaired increases.

    When liver function is impaired due to various factors, aspartate aminotransferase (also referred to as glutamate-oxaloacetate transaminase, abbreviated as GOT) or alanine aminotransferase (glutamate-pyruvate transaminase) present in hepatocytes. And abbreviated as GPT), etc., leak into the blood, and thus GOT activity and GPT activity in blood are indicators of liver dysfunction.

    Although hepatic dysfunction can be treated with medicine, it is desirable to prevent hepatic dysfunction to a minimum, preferably by prophylactic measures. From this point of view, it can be said that foods and food ingredients that can be ingested on a daily basis are most suitable as preventive measures. As foods that suppress liver dysfunction, turmeric, oyster extract, liver extract and the like are known.

In recent years, research on the physiological functions of foods and food ingredients has been actively conducted, and cheese has also been shown to promote the lowering of blood triglyceride concentration and the improvement of cholesterol metabolism, despite being a high-fat food. Have been reported (Patent Document 1, Patent Document 2). It has also been reported that it has an action of suppressing oxidative damage caused by active oxygen, free radicals and the like that adversely affect diseases, aging, etc. in the living body (Patent Document 3).
JP 2003-300890 A JP 2003-144090 A JP 2004-352958 A

    The present invention is useful for suppressing liver dysfunction when ingested, and further, a liver dysfunction inhibitor comprising as an active ingredient an ingredient contained in cheese that can be taken daily, or for suppressing liver dysfunction An object is to provide food and drink.

    In view of whether various functional abnormalities of the living body can be prevented or improved by foods and food components that can be ingested on a daily basis, the present inventors have focused on the components contained in cheese and confirmed their physiological functions. As a result, the present inventors have found an action to suppress liver dysfunction in vivo. And it discovered that the disorder | damage | failure of a liver function could be suppressed by providing the liver dysfunction inhibitor which utilized this physiological function, or the food-drinks for liver dysfunction disorder, and came to complete this invention. In other words, using the action of suppressing the impairment of liver function of ingredients contained in cheese, for inhibiting liver dysfunction containing an ingredient contained in cheese as an active ingredient, or containing ingredients contained in cheese The problem could be solved by providing food and drink.

    In addition, cheese is a food that has been eaten since ancient times, and it can be said that there is no problem in safety even if it is taken daily, but the consumption of cheese in Japan is as low as about 5 to 6 g per day. .

    Ingredients that can be used in the present invention are usually eaten cheeses such as gouda cheese, cheddar cheese, emmental cheese, edam cheese, camembert, stilton cheese, blue cheese and other natural cheeses, or these natural cheeses. It is contained in processed cheese processed as a raw material. And cheese itself can also be used as a component contained in cheese. In addition, after suspending cheese in a solvent, fractions that have been degreased, insoluble matter removed, protein removed, etc. can be used. A fraction purified by chromatography can also be used. These fractions can also be used after being concentrated or dried.

    By the way, the maturity (%) indicating the degree of ripening of the cheese is calculated by the formula of [(soluble nitrogen / total nitrogen) × 100], and is about 6 to 7% immediately after the production of natural cheese. Although there is also cheese that rises as it progresses and becomes about 24 to 30%, in the present invention, it is confirmed that the inhibitory effect of liver dysfunction increases as the ripening of cheese progresses and the degree of maturity increases. This means that the components effective for the liver dysfunction-inhibiting action increase with the ripening of the cheese. Therefore, in this invention, it is desirable to use the component which increases as cheese matures as a component contained in cheese.

    Examples of the dosage form of the liver dysfunction inhibitor of the present invention include preparations such as tablets, capsules, granules, powders, powders, and syrups. Examples of foods and drinks for suppressing liver dysfunction include breads, snacks, cakes, puddings, beverages, fermented milk, noodles, sausages, various milk powders and baby foods.

    The dose of the liver dysfunction inhibitor of the present invention may be appropriately determined in consideration of the purpose of treatment and prevention, symptoms, weight, age, sex and the like, but usually about 20 to 200 g for cheese itself. It is desirable to be able to ingest.

    Since the liver dysfunction inhibitor and the food and drink for suppressing liver dysfunction according to the present invention can suppress liver function disorder by ingesting these, acute hepatitis, chronic hepatitis, viral hepatitis, alcoholic fatty liver It is useful for the prevention and improvement of diseases such as cirrhosis and liver cancer.

    The liver dysfunction inhibitor of this invention makes the component contained in cheese an active ingredient. Moreover, the food-drinks for liver dysfunction suppression of this invention mix | blend the component which has the liver dysfunction inhibitory effect contained in cheese. And the liver dysfunction inhibitor of the present invention is tablets, capsules, granules, powders, powders, syrups and the like, and foods and drinks for liver dysfunction inhibition are bread, snacks, cakes, puddings, beverages, Fermented milk, noodles, sausages, various powdered milk, baby food, etc.

    In order to exert a liver dysfunction inhibitory action, in the case of an adult, an active ingredient corresponding to about 20 to 200 g of cheese per serving can be ingested.

  Hereinafter, the present invention will be described in more detail with reference to Examples and Test Examples. However, these are merely illustrative and the present invention is not limited thereto.

(Preparation of bacteria ripening type cheese)
The raw milk was sterilized by heating (75 ° C., 15 seconds), cooled to 30 ° C., and 0.01% calcium chloride was added. Furthermore, 0.7% of commercially available lactic acid bacteria starter (LD starter, Christian Hansen) and 1% of Lactobacillus helveticus SBT2171 (FERMP-14381) of polysaccharide-producing lactic acid bacteria were added, and Rennet 0.003 % Was added to coagulate the milk. The curd thus obtained was cut and stirred until the pH was 6.2 to 6.1, and whey was discharged to obtain curd grains. Then, the curd grains were packed and pressed, further salted and aged at 10 ° C. to prepare Gouda cheese type hard natural cheese.

[Test Example 1]
(Confirmation of liver dysfunction inhibitory effect)
Using the ripened 5-month cheese (ripening 30%) and the ripened 8-month cheese (ripening 38%) produced in Example 1, the action of suppressing liver dysfunction due to alcohol was confirmed. Moreover, unripe cheese was used as a control. Animal experiments consisted of 8 animals per group, a group administered with aged 5 months cheese (aged 5 months group), a group administered with aged 8 months cheese (aged 8 months group), and a group administered with unripened cheese (unaged) Group).

    The animal experiment was based on the liquid feed method (Lin, Liquid diet preparation for study of chronic alcohol ingestion in the rat, Lab. Anim. Sci., Vol.39, p.618-620, 1989). That is, a liquid feed containing 20% of each cheese, 8.5% of sucrose and 5% of ethanol was prepared and administered to C57BL / 6 mice (5 weeks old, male, Charles River Japan, Inc.) for 1 week. Thereafter, GOT activity and GPT activity leaked into the blood from damaged hepatocytes were measured with Fuji Dry Chem FDC5500 system. The intake of liquid feed was 8.8 g ± 0.4 g on average per mouse per day, and there was no difference between groups.

    The results are shown in FIGS. According to this, the GOT activity and the GPT activity were significantly lower values in both the aging 5 month group and the aging 8 month group than in the unaged group. That is, it was found that liver dysfunction can be reduced by ingesting components that increase as cheese matures.

(Preparation of mold ripening type cheese)
The raw milk is sterilized by heating (75 ° C, 15 seconds), then cooled to 30 ° C, and 2% commercial lactic acid bacteria starter (LD starter, Christian Hansen) 2% and 0.003% rennet are added to solidify the milk. It was. The curd thus obtained was cut into a 15 mm square, held for 30 minutes, filled into a cylindrical mold (diameter 110 mm, height 30 mm), and held for 19 hours to eliminate whey. Next, after the curd was taken out of the mold, it was immersed in a 23% salt solution for salting, and a dispersion containing Penicillium caseilum spores was sprayed uniformly on the curd surface. Then, after drying in a drying room (14 ° C., humidity 75%) for 1 day, it is aged in an aging room (14 ° C., humidity 95%) for 7 days, and further in an aging room (5 ° C., humidity 75%) for 21 days. Aged to produce white mold-based natural cheese. In addition, the maturity of this cheese was 27%.

  About the obtained white mold type | system | group natural cheese, using an immature cheese as a control | contrast, when the effect | action which suppresses the liver dysfunction by alcohol was confirmed by the animal experiment method similar to Test Example 1, it turned out that liver dysfunction can be reduced. It was.

    As an active ingredient contained in cheese, 0.5 kg of ripened 8-month gouda cheese obtained in Example 1 and 0.5 kg of cheddar cheese are mixed, and 20 g of molten salt sodium polyphosphate and 110 ml of water are added, and a kettle emulsifier ( Steam was blown while rotating at a rotation speed of 120 rpm, and the temperature was raised to 85 ° C. to emulsify. And this emulsified cheese was filled into cartons and stored refrigerated at 5 ° C. to obtain a processed cheese for inhibiting liver dysfunction.

    200 ml of milk and 2.5 g of powdered agar were mixed well and heated. When the agar was melted, 50 g of sugar was added and boiled and lightly boiled. And as an active ingredient contained in cheese, 150 g of ripened 8-month Gouda cheese obtained in Example 1 is added to a bite, takes heat, casts into a mold, hardens in a refrigerator, and suppresses liver dysfunction. I got the Yokan.

    As an active ingredient contained in cheese, after chopping 10 kg of aged 8 month gouda cheese obtained in Example 1 with a meat chopper, molten salt (1.2% sodium phosphate, 1% sodium polyphosphate) and an emulsifier ( The mixture was poured into a dissolution tank containing 5 kg of 90 ° C. hot water together with 0.6% sugar ester, adjusted to pH 6.2, stirred, and blown with steam to prepare an emulsion. And this emulsion was homogenized, filtered with a filter, and spray-dried to obtain a powdered cheese for inhibiting liver dysfunction.

    100 g of powdered cheese for inhibiting liver dysfunction obtained in Example 5, 40 g of vitamin C, 100 g of granulated sugar and 60 g of an equal mixture of corn starch and lactose were mixed and packed in a bag to obtain a nutritional health food for inhibiting liver dysfunction. .

FIG. 4 is a diagram showing the measurement results of blood GOT activity in each experimental group in Test Example 1. FIG. 3 is a diagram showing the results of measuring blood GPT activity in each experimental group in Test Example 1.

Claims (6)

    A liver dysfunction inhibitor comprising as an active ingredient an ingredient contained in cheese.     The liver dysfunction inhibitor according to claim 1, wherein the component contained in the cheese is a component that increases as the cheese matures.     The liver dysfunction inhibitor of Claim 1 or 2 which used cheese itself.     A food or drink for suppressing liver dysfunction containing ingredients contained in cheese.     The foodstuff for liver function disorder | damage | failure suppression of Claim 4 which is a component which the component contained in cheese increases according to ripening of cheese. The food / beverage product for liver dysfunction suppression of Claim 4 which mix | blended cheese itself.

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