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JP2015006136A - Gravity-changing apparatus for cell culture - Google Patents

Gravity-changing apparatus for cell culture Download PDF

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JP2015006136A
JP2015006136A JP2013132109A JP2013132109A JP2015006136A JP 2015006136 A JP2015006136 A JP 2015006136A JP 2013132109 A JP2013132109 A JP 2013132109A JP 2013132109 A JP2013132109 A JP 2013132109A JP 2015006136 A JP2015006136 A JP 2015006136A
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rotating body
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JP6249361B2 (en
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弓削　類
Rui Yuge
類 弓削
裕美 河原
Hiromi Kawahara
裕美 河原
文敬 山崎
Fumitaka Yamazaki
文敬 山崎
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IXS RESEARCH KK
Space Bio Laboratories Co Ltd
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Space Bio Laboratories Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure

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Abstract

PROBLEM TO BE SOLVED: To provide a gravity-changing apparatus for cell culture, capable of reducing a running cost without including any contact-type power-feeding mechanism.SOLUTION: A gravity-changing apparatus 1 for cell culture includes: a first shaft 11a that rotates due to the driving of a first driving device 12; a first rotating body 10 that is attached to the first shafts 11a, 11b, and that rotates about the first shafts 11a, 11b; a second shaft 21a that is attached to the first rotating body 10 and that rotates due to the driving of a second driving device 22; a second rotating body 20 that has a culture container attached thereto, is attached to the second shafts 21a, 21b, and rotates about the second shafts 21a, 21b in the interior region of the rotation region of the first rotating body 10; and a transmission mechanism that is disposed on the first rotating body 10, and that transmits rotational force from the second driving device 22 to the second shaft 21a.

Description

本発明は、細胞培養用重力変動装置に関する。   The present invention relates to a gravity fluctuation device for cell culture.

宇宙での細胞培養実験等から生物の成長は重力に大きく影響を受けることが知られている。地球上においても無重力環境下或いは低重力環境下で細胞培養実験を行い得るよう、重力を変動させて擬似的に微小重力を発生させ得る装置が提案されている。   It is known from the cell culture experiments in space that the growth of organisms is greatly affected by gravity. An apparatus capable of generating microgravity in a pseudo manner by changing the gravity has been proposed so that cell culture experiments can be performed on the earth in a zero-gravity environment or a low-gravity environment.

例えば、特許文献１〜３に開示されているように、第１回転軸まわりに回転する第１回転体と第１回転軸に直交する第２回転軸まわりに回転する第２回転体とから構成され、３次元的に培養容器を回転させる装置がある。   For example, as disclosed in Patent Documents 1 to 3, a first rotating body that rotates around a first rotating shaft and a second rotating body that rotates around a second rotating shaft perpendicular to the first rotating shaft are configured. In addition, there is an apparatus for rotating a culture vessel in three dimensions.

特開２００３−９８５２号公報Japanese Patent Laid-Open No. 2003-9852 特開２０１０−１９３９１０号公報JP 2010-193910 A 特開２０１０−２４６４３４号公報JP 2010-246434 A

特許文献１〜３に開示されている装置では、内側の回転体を回転させるモータが外側の回転体に設置されていることから、モータ自身も回転している。このため、モータへは外部よりカップリングを介して給電する必要がある。カップリングは、外側の回転体が回転している最中でも内側の回転体に設置されたモータとの電力のやり取りができるよう、スリップリング及びこれに接触するブラシで構成されている。このような接触式の給電機構の場合、スリップリング及びブラシの摩耗が生じることから、定期的な部品の交換等のメンテナンスが要求され、ランニングコストが高いという課題がある。   In the devices disclosed in Patent Documents 1 to 3, since the motor that rotates the inner rotating body is installed in the outer rotating body, the motor itself is also rotating. For this reason, it is necessary to supply electric power to the motor from outside via a coupling. The coupling is composed of a slip ring and a brush in contact with the slip ring so that power can be exchanged with a motor installed on the inner rotating body even while the outer rotating body is rotating. In the case of such a contact type power supply mechanism, wear of the slip ring and the brush occurs, so that maintenance such as periodic replacement of parts is required, and there is a problem that running cost is high.

本発明は上記事項に鑑みてなされたものであり、その目的は、接触式の給電機構を備えずランニングコストの低減が可能な細胞培養用重力変動装置を提供することにある。   The present invention has been made in view of the above matters, and an object of the present invention is to provide a gravity variator for cell culture that does not include a contact-type power supply mechanism and can reduce running costs.

本発明に係る細胞培養用重力変動装置は、
第１駆動装置の駆動によって回転する第１軸と、
前記第１軸に取り付けられて前記第１軸の回りを回転する第１回転体と、
前記第１回転体に取り付けられ第２駆動装置の駆動により回転する第２軸と、
前記第２軸に取り付けられ、前記第１回転体の回転領域の内側領域にて前記第２軸の回りを回転し、培養容器が取り付けられる第２回転体と、
前記第１回転体に配置され、前記第２駆動装置から前記第２軸に回転力を伝達する伝達機構と、を備え、
前記第１駆動装置及び前記第２駆動装置が前記第１回転体の回転領域の外側領域に配置され、
前記伝達機構によって前記第２駆動装置から回転軸の軸方向が複数回変換されつつ前記第２軸へ伝達されて前記第２軸と前記第１軸とが直交している、
ことを特徴とする。 The gravity variability apparatus for cell culture according to the present invention comprises:
A first shaft that is rotated by driving of the first drive device;
A first rotating body attached to the first shaft and rotating around the first shaft;
A second shaft attached to the first rotating body and rotated by driving of a second driving device;
A second rotating body attached to the second shaft, rotating around the second axis in an inner region of the rotation region of the first rotating body, and a culture vessel being attached thereto;
A transmission mechanism disposed on the first rotating body and configured to transmit a rotational force from the second driving device to the second shaft;
The first driving device and the second driving device are disposed in an outer region of a rotation region of the first rotating body;
The second mechanism is transmitted to the second axis while the axial direction of the rotating shaft is converted a plurality of times by the transmission mechanism, and the second axis and the first axis are orthogonal to each other.
It is characterized by that.

また、前記伝達機構が前記第１回転体の内部に配置されていることが好ましい。   Moreover, it is preferable that the transmission mechanism is disposed inside the first rotating body.

また、前記伝達機構として傘歯車を備え、
前記傘歯車によって回転軸の軸方向が変換されることが好ましい。 The transmission mechanism includes a bevel gear,
It is preferable that the axial direction of the rotating shaft is changed by the bevel gear.

また、同一平面内にて回転軸の軸方向が少なくとも３回変換されることが好ましい。   Moreover, it is preferable that the axial direction of a rotating shaft is converted at least 3 times within the same plane.

本発明に係る細胞培養用重力変動装置では、接触式の給電機構を備えないので、給電機構の定期的な交換等のメンテナンスが不要であり、ランニングコストの低減が可能である。   Since the gravity variability apparatus for cell culture according to the present invention does not include a contact-type power supply mechanism, maintenance such as periodic replacement of the power supply mechanism is unnecessary, and running costs can be reduced.

細胞培養用重力変動装置の平面図である。It is a top view of the gravity fluctuation | variation apparatus for cell cultures. 細胞培養用重力変動装置の正面図である。It is a front view of the gravity fluctuation apparatus for cell cultures. 図１のＡ−Ａ’部分断面図であり、細胞培養用重力変動装置の内部構造図である。It is A-A 'partial sectional drawing of FIG. 1, and is an internal structure figure of the gravity fluctuation apparatus for cell cultures. 細胞培養用重力変動装置が作動している状態を示す斜視図である。It is a perspective view which shows the state which the gravity fluctuation apparatus for cell cultures has act | operated. 他の形態に係る細胞培養用重力変動装置の内部構造を示す部分断面図である。It is a fragmentary sectional view which shows the internal structure of the gravity fluctuation apparatus for cell cultures which concerns on another form. 他の形態に係る細胞培養用重力変動装置の内部構造を示す部分断面図である。It is a fragmentary sectional view which shows the internal structure of the gravity fluctuation apparatus for cell cultures which concerns on another form.

以下、図を参照しつつ、本実施の形態に係る細胞培養用重力変動装置について説明する。細胞培養用重力変動装置１は、図１〜図３に示すように、第１回転体１０、第１軸１１ａ、１１ｂ、第１駆動装置収容部３２に収容された第１駆動装置１２、第２回転体２０、第２軸２１ａ、２１ｂ、第２駆動装置収容部３３に収容された第２駆動装置２２、支持台３０、支持部材３１ａ、３１ｂを備える。   Hereinafter, the gravity fluctuation device for cell culture according to the present embodiment will be described with reference to the drawings. As shown in FIGS. 1 to 3, the cell culture gravity fluctuation device 1 includes a first rotating device 10, first shafts 11 a and 11 b, a first driving device 12 housed in a first driving device housing portion 32, a first driving device 12. The two-rotary body 20, the second shafts 21a and 21b, the second drive device 22 accommodated in the second drive device accommodating portion 33, the support base 30, and the support members 31a and 31b are provided.

支持台３０から支持部材３１ａ、３１ｂが対向して立設している。この支持部材３１ａ、３１ｂの間に第１回転体１０、第２回転体２０等が支持されている。   Support members 31a and 31b are erected from the support base 30 so as to face each other. The first rotating body 10, the second rotating body 20, and the like are supported between the support members 31a and 31b.

第１回転体１０は、第１軸１１ａ、１１ｂに軸支されている。第１軸１１ａ、１１ｂは同軸（Ｘ軸）上に配置されている。第１軸１１ａの一端が第１回転体１０と連結しており、また、他端が第１駆動装置１２の出力軸１２ａに連結している。第１軸１１ｂは、支持部材３１ａに固定されて第１回転体１０が摺動可能な形態であっても、第１回転体１０に固定されて支持部材３１ａに対して摺動可能な形態であってもよい。このような構成により、第１駆動装置１２が駆動すると出力軸１２ａに連結した第１軸１１ａが回転し、第１回転体１０がＸ軸まわりに回転する。   The first rotating body 10 is pivotally supported by the first shafts 11a and 11b. The first shafts 11a and 11b are arranged on the same axis (X axis). One end of the first shaft 11 a is connected to the first rotating body 10, and the other end is connected to the output shaft 12 a of the first driving device 12. Even if the first shaft 11b is fixed to the support member 31a and the first rotating body 10 is slidable, the first shaft 11b is fixed to the first rotating body 10 and slidable with respect to the support member 31a. There may be. With such a configuration, when the first driving device 12 is driven, the first shaft 11a connected to the output shaft 12a rotates, and the first rotating body 10 rotates around the X axis.

第１回転体１０は、ここでは矩形の枠体であり、第１回転体１０の回転領域の内側領域には、第２回転体２０が配置されて回転可能な程度の空間を有している。   Here, the first rotating body 10 is a rectangular frame, and the inner area of the rotating area of the first rotating body 10 has a space that allows the second rotating body 20 to be disposed and rotated. .

第１回転体１０の対向する枠に、第２軸２１ａ、２１ｂがそれぞれ取り付けられている。第２軸２１ａ、２１ｂは同軸（Ｙ軸）上に配置されている。この第２軸２１ａ、２１ｂに第２回転体２０が取り付けられている。   The second shafts 21a and 21b are attached to the opposing frames of the first rotating body 10, respectively. The second shafts 21a and 21b are arranged on the same axis (Y axis). The second rotating body 20 is attached to the second shafts 21a and 21b.

第２軸２１ａの一端は第２回転体２０に連結しており、また、他端が傘歯車２８に連結している。傘歯車２８が回転すると、第２回転体２０は第１回転体１０の内側領域にて回転する。第２軸２１ｂは、一端が第２回転体２０に連結しており、また、他端は第１回転体１０に摺動可能に取り付けられている。   One end of the second shaft 21 a is connected to the second rotating body 20, and the other end is connected to the bevel gear 28. When the bevel gear 28 rotates, the second rotating body 20 rotates in the inner region of the first rotating body 10. One end of the second shaft 21 b is connected to the second rotating body 20, and the other end is slidably attached to the first rotating body 10.

第２回転体２０は、細胞培養用の密封型の培養容器が取り付けられる場であり、培養容器の取り付けは、第２回転体２０の任意の箇所に紐やゴム、固定具などを用いて固定される。また、第２回転体２０には、細胞容器取り付け用の取り付け部が形成されていてもよい。   The second rotating body 20 is a place where a sealed culture container for cell culture is attached, and the culture container is fixed to an arbitrary portion of the second rotating body 20 using a string, rubber, a fixture, or the like. Is done. Further, the second rotating body 20 may be formed with an attachment portion for attaching the cell container.

第２駆動装置２２は、支持部材３１ｂに設置されている。第２駆動装置２２の出力軸２２ａが第１軸１１ａ、１１ｂと平行に設置されており、出力軸２２ａには、歯車２３が設置されている。歯車２３は、第１軸１１ｂに摺動可能に取り付けられた歯車２４と噛み合うよう設置されている。   The second drive device 22 is installed on the support member 31b. An output shaft 22a of the second drive device 22 is installed in parallel with the first shafts 11a and 11b, and a gear 23 is installed on the output shaft 22a. The gear 23 is installed so as to mesh with a gear 24 slidably attached to the first shaft 11b.

歯車２４は第１回転体１０の内部に配置される傘歯車２５と一体的に形成されている。歯車２４及び傘歯車２５には第１軸１１ｂが貫通しており、歯車２４及び傘歯車２５が第１軸１１ｂに対して摺動可能に構成されている。   The gear 24 is formed integrally with a bevel gear 25 disposed inside the first rotating body 10. The first shaft 11b passes through the gear 24 and the bevel gear 25, and the gear 24 and the bevel gear 25 are configured to be slidable with respect to the first shaft 11b.

第１回転体１０の内部には、第２駆動装置２２からの駆動力を第２軸２１ａへ伝達する回転力伝達部材２６、２７が配置されている。   Inside the first rotating body 10, rotational force transmitting members 26 and 27 for transmitting the driving force from the second driving device 22 to the second shaft 21a are arranged.

回転力伝達部材２６は、シャフト２６ｂの両端に傘歯車２６ａ、２６ｃがそれぞれ取り付けられて構成されている。シャフト２６ｂは、第１回転体１０に摺動可能に設置されるとともに、第１軸１１ａに対して垂直に（第２軸２１ａ、２１ｂに対して平行に）設置されている。   The rotational force transmission member 26 is configured by attaching bevel gears 26a and 26c to both ends of a shaft 26b. The shaft 26b is slidably installed on the first rotating body 10, and is installed perpendicular to the first shaft 11a (parallel to the second shafts 21a and 21b).

一方、回転力伝達部材２７は、シャフト２７ｂの両端に傘歯車２７ａ、２７ｃがそれぞれ取り付けられて構成されている。シャフト２７ｂは、第１回転体１０に摺動可能に設置されるとともに、第１軸１１ａに対して平行に（第２軸２１ａ、２１ｂに対して垂直に）設置されている。   On the other hand, the rotational force transmitting member 27 is configured by attaching bevel gears 27a and 27c to both ends of a shaft 27b. The shaft 27b is slidably disposed on the first rotating body 10, and is disposed in parallel to the first shaft 11a (perpendicular to the second shafts 21a and 21b).

回転力伝達部材２６の傘歯車２６ｃは第１軸１１ｂに取り付けられている傘歯車２５と噛み合っている。また、傘歯車２６ａは、回転力伝達部材２７の傘歯車２７ａと噛み合っている。また、傘歯車２７ｃは第２回転体２０に連結している第２軸２１ａに取り付けられた傘歯車２８と噛み合っている。   The bevel gear 26c of the rotational force transmitting member 26 is meshed with the bevel gear 25 attached to the first shaft 11b. Further, the bevel gear 26 a meshes with the bevel gear 27 a of the rotational force transmission member 27. Further, the bevel gear 27 c meshes with a bevel gear 28 attached to the second shaft 21 a connected to the second rotating body 20.

第１駆動装置１２及び第２駆動装置２２は、第１回転体１０、第２回転体２０に回転力を供給可能な電動の駆動装置が用いられ、例えば、出力軸１２ａ、２２ａの回転を高精度に制御可能なサーボモータ、ステッピングモータ等のモータが用いられる。   As the first driving device 12 and the second driving device 22, an electric driving device capable of supplying a rotational force to the first rotating body 10 and the second rotating body 20 is used. For example, the rotation of the output shafts 12a and 22a is increased. Motors such as servo motors and stepping motors that can be accurately controlled are used.

なお、図示を省略しているが、上記構成における摺動箇所については、それぞれボールベアリング等の摺動部材が設置されていることが好ましい。   In addition, although illustration is abbreviate | omitted, it is preferable that sliding members, such as a ball bearing, are each installed about the sliding location in the said structure.

続いて、細胞培養用重力変動装置１の動作を説明する。図４に、第１回転体１０及び第２回転体２０がそれぞれ回転している状態を示す。上述した構成によって、第１駆動装置１２の駆動により、出力軸１２ａ、第１軸１１ａがＸ軸まわりに回転するので、第１回転体１０がＸ軸まわりに回転する。   Next, the operation of the cell culture gravity fluctuation device 1 will be described. FIG. 4 shows a state where the first rotating body 10 and the second rotating body 20 are rotating. With the above-described configuration, the output shaft 12a and the first shaft 11a rotate around the X axis by driving the first driving device 12, so that the first rotating body 10 rotates around the X axis.

また、第２駆動装置２２の駆動により、出力軸２２ａ、歯車２３が回転し、歯車２４、傘歯車２５が回転する。そして、回転力伝達部材２６、回転力伝達部材２７が回転し、傘歯車２８及び第２軸２１ａに伝達され、第２回転体２０が回転する。このように、第２駆動装置２２の出力軸２２ａから、回転軸の軸方向が同一平面内で３回変換されて第２回転体２０に回転力が伝達され、第２回転体２０が回転している。   Further, the output shaft 22a and the gear 23 are rotated by the driving of the second driving device 22, and the gear 24 and the bevel gear 25 are rotated. Then, the rotational force transmitting member 26 and the rotational force transmitting member 27 rotate and are transmitted to the bevel gear 28 and the second shaft 21a, and the second rotating body 20 rotates. As described above, the axial direction of the rotating shaft is converted three times in the same plane from the output shaft 22a of the second driving device 22 to transmit the rotational force to the second rotating body 20, and the second rotating body 20 rotates. ing.

第１軸１１ａ、１１ｂと第２軸２１ａ、２１ｂは、第１回転体１０及び第２回転体２０の回転中も絶えず垂直な関係が維持される。第２回転体２０に取り付けられる培養容器には、直交する２軸（第１軸１１ａ、１１ｂ及び第２軸２１ａ、２１ｂ）まわりに回転することから、細胞容器には３次元的な回転が与えられる。このため、培養容器に作用する重力は四方八方へほぼ均等に分散される。そして、培養容器に作用する重力を単位時間当たりで平均すると、培養容器をほぼ無重力環境下（１／１０００Ｇ程度）に置いた状態にすることができる。   The first shafts 11a and 11b and the second shafts 21a and 21b are constantly maintained in a vertical relationship while the first rotating body 10 and the second rotating body 20 are rotating. Since the culture vessel attached to the second rotating body 20 rotates around two orthogonal axes (first axis 11a, 11b and second axis 21a, 21b), the cell container is given a three-dimensional rotation. It is done. For this reason, the gravity which acts on a culture container is disperse | distributed substantially equally in all directions. When the gravity acting on the culture vessel is averaged per unit time, the culture vessel can be placed in a substantially weightless environment (about 1/1000 G).

また、第１回転体１０及び第２回転体２０のそれぞれの回転速度を調整することで、上記の擬似的な無重力環境のほか、１／６Ｇである月での重力環境や、２Ｇや３Ｇなど１Ｇを超える荷重力環境など多彩な重力環境を擬似的に再現することができる。   Further, by adjusting the respective rotational speeds of the first rotating body 10 and the second rotating body 20, in addition to the above-described pseudo zero gravity environment, the gravity environment in the moon of 1 / 6G, 2G, 3G, etc. Various gravity environments such as a load force environment exceeding 1G can be simulated.

また、第１回転体１０、第２回転体２０は、それぞれ独立して駆動する第１駆動装置１２、第２駆動装置２２により回転する。したがって、第１駆動装置１２及び第２駆動装置２２を独立に制御することで、第１回転体１０及び第２回転体２０の回転速度をそれぞれ独立に調整することが可能である。   Moreover, the 1st rotary body 10 and the 2nd rotary body 20 rotate by the 1st drive device 12 and the 2nd drive device 22 which drive independently, respectively. Therefore, by independently controlling the first driving device 12 and the second driving device 22, the rotational speeds of the first rotating body 10 and the second rotating body 20 can be independently adjusted.

また、第２回転体２０を回転させる第２駆動装置２２は、第１回転体１０の回転領域の外側に配置されていることから、第２駆動装置２２への給電にスリップリング及びブラシから構成されるカップリング装置等、接触式の給電機構を備えていない。このため、カップリング装置の交換等のメンテナンスが不要であり、ランニングコストの低減が可能である。   In addition, since the second driving device 22 that rotates the second rotating body 20 is disposed outside the rotation region of the first rotating body 10, the second driving device 22 includes a slip ring and a brush for supplying power to the second driving device 22. It does not have a contact-type power supply mechanism such as a coupling device. For this reason, maintenance such as replacement of the coupling device is unnecessary, and the running cost can be reduced.

本発明は、本発明の広義の精神と範囲を逸脱することなく、様々な実施の形態及び変形が可能とされるものである。また、上述した実施の形態は、本発明を説明するためのものであり、本発明の範囲を限定するものではない。すなわち、本発明の範囲は、実施の形態ではなく、特許請求の範囲によって示される。そして、特許請求の範囲内及びそれと同等の発明の意義の範囲内で施される様々な変形が、本発明の範囲内とみなされる。   Various embodiments and modifications can be made to the present invention without departing from the broad spirit and scope of the present invention. The above-described embodiments are for explaining the present invention and do not limit the scope of the present invention. In other words, the scope of the present invention is shown not by the embodiments but by the claims. Various modifications within the scope of the claims and within the scope of the equivalent invention are considered to be within the scope of the present invention.

例えば、図５に示す細胞培養用重力変動装置２のように、支持台３０からＬ字状の支持部材３１が立設し、支持台３０と支持部材３１の間に第１回転体１０及び第２回転体２０が支持された形態であってもよい。この場合、第１軸１１ａ、１１ｂがＹ軸上に配置されて第１回転体１０がＹ軸まわりに回転する。また、第２軸２１ａ、２１ｂがＸ軸上に配置され第２回転体２０がＸ軸まわりに回転する。   For example, as in the cell culture gravity fluctuation device 2 shown in FIG. 5, an L-shaped support member 31 is erected from the support base 30, and the first rotating body 10 and the The two-rotor 20 may be supported. In this case, the first shafts 11a and 11b are arranged on the Y axis, and the first rotating body 10 rotates around the Y axis. Further, the second shafts 21a and 21b are arranged on the X axis, and the second rotating body 20 rotates around the X axis.

また、図６に示す細胞培養用重力変動装置３のように、回転力伝達部材２６のかわりに、歯車２４からの回転力をベルト２９で回転力伝達部材２７へ伝達するよう構成されていてもよい。また、同様に、回転力伝達部材２７のかわりに、回転力伝達部材２６からの回転力を第２軸２１ａに伝達するよう構成されていてもよい。   Further, as in the gravity fluctuation device 3 for cell culture shown in FIG. 6, the rotational force from the gear 24 may be transmitted to the rotational force transmitting member 27 by the belt 29 instead of the rotational force transmitting member 26. Good. Similarly, instead of the rotational force transmitting member 27, the rotational force from the rotational force transmitting member 26 may be transmitted to the second shaft 21a.

１〜３ 細胞培養用重力変動装置
１０ 第１回転体
１１ａ、１１ｂ 第１軸
１２ 第１駆動装置
１２ａ 出力軸
２０ 第２回転体
２１ａ、２１ｂ 第２軸
２２ 第２駆動装置
２２ａ 出力軸
２３ 歯車
２４ 歯車
２５ 傘歯車
２６ 回転力伝達部材
２６ａ 傘歯車
２６ｂ シャフト
２６ｃ 傘歯車
２７ 回転力伝達部材
２７ａ 傘歯車
２７ｂ シャフト
２７ｃ 傘歯車
２８ 傘歯車
２９ ベルト
３０ 支持台
３１、３１ａ、３１ｂ 支持部材
３２ 第１駆動装置収容部
３３ 第２駆動装置収容部 1-3 Gravity fluctuation device for cell culture 10 1st rotating body 11a, 11b 1st shaft 12 1st drive device 12a Output shaft 20 2nd rotating body 21a, 21b 2nd shaft 22 2nd drive device 22a Output shaft 23 Gear 24 Gear 25 Bevel gear 26 Rotational force transmission member 26a Bevel gear 26b Shaft 26c Bevel gear 27 Rotational force transmission member 27a Bevel gear 27b Shaft 27c Bevel gear 28 Bevel gear 29 Belt 30 Support base 31, 31a, 31b Support member 32 First drive device Housing unit 33 Second drive unit housing unit

Claims (4)

第１駆動装置の駆動によって回転する第１軸と、
前記第１軸に取り付けられて前記第１軸の回りを回転する第１回転体と、
前記第１回転体に取り付けられ第２駆動装置の駆動により回転する第２軸と、
前記第２軸に取り付けられ、前記第１回転体の回転領域の内側領域にて前記第２軸の回りを回転し、培養容器が取り付けられる第２回転体と、
前記第１回転体に配置され、前記第２駆動装置から前記第２軸に回転力を伝達する伝達機構と、を備え、
前記第１駆動装置及び前記第２駆動装置が前記第１回転体の回転領域の外側領域に配置され、
前記伝達機構によって前記第２駆動装置から回転軸の軸方向が複数回変換されつつ前記第２軸へ伝達されて前記第２軸と前記第１軸とが直交している、
ことを特徴とする細胞培養用重力変動装置。 A first shaft that is rotated by driving of the first drive device;
A first rotating body attached to the first shaft and rotating around the first shaft;
A second shaft attached to the first rotating body and rotated by driving of a second driving device;
A second rotating body attached to the second shaft, rotating around the second axis in an inner region of the rotation region of the first rotating body, and a culture vessel being attached thereto;
A transmission mechanism disposed on the first rotating body and configured to transmit a rotational force from the second driving device to the second shaft;
The first driving device and the second driving device are disposed in an outer region of a rotation region of the first rotating body;
The second mechanism is transmitted to the second axis while the axial direction of the rotating shaft is converted a plurality of times by the transmission mechanism, and the second axis and the first axis are orthogonal to each other.
A gravity fluctuation device for cell culture characterized by the above. 前記伝達機構が前記第１回転体の内部に配置されている、
ことを特徴とする請求項１に記載の細胞培養用重力変動装置。 The transmission mechanism is disposed inside the first rotating body;
The gravity fluctuation apparatus for cell culture according to claim 1. 前記伝達機構として傘歯車を備え、
前記傘歯車によって回転軸の軸方向が変換される、
ことを特徴とする請求項１又は２に記載の細胞培養用重力変動装置。 A bevel gear is provided as the transmission mechanism,
The axial direction of the rotating shaft is converted by the bevel gear,
The gravity variability apparatus for cell culture according to claim 1 or 2. 同一平面内にて回転軸の軸方向が少なくとも３回変換される、
ことを特徴とする請求項１乃至３のいずれか一項に記載の細胞培養用重力変動装置。 The axial direction of the rotation axis is converted at least three times in the same plane,
The gravity fluctuation device for cell culture according to any one of claims 1 to 3.
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