JP3231898B2 - Whitening cosmetics - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a whitening cosmetic having a skin whitening effect, and more particularly to a whitening composition containing, as an active ingredient, a flower blood wisteria (crude) extract having a whitening effect based on a melanin production inhibiting action. Related to cosmetics.

[0002]

2. Description of the Related Art In general, it is considered that darkening, spots, freckles, and the like due to sunburn are caused by the production of melanin, which is a black-brown amorphous pigment, and this melanin causes external stimuli such as ultraviolet rays on the skin. When it is received, tyrosinase (tyrosine oxidase) present in the melanocytes of the skin is activated, and tyrosine, one of the amino acids constituting protein, is oxidized and produced. From this, an effect of whitening the skin is expected by suppressing the activity of tyrosinase involved in melanin production. Therefore, it has been proposed to add a tyrosinase activity-inhibiting component to cosmetics.

Conventionally, as a whitening cosmetic having a whitening effect, as disclosed in JP-B-55-43443, "Whitening cosmetic" and JP-B-54-974, "Composition of crude drug extract", ascorbic acid or What blended the derivative is known. In addition, skin external preparations containing arbutin (JP-A-60-16906), bleaching cosmetics containing kojic acid (Japanese Patent Publication No. 32-8100), plant components (JP-A-63-2913, etc.) ) Or animal components (JP-A-63-8312, etc.) are known as having a whitening effect.

[0004] However, many of the above-mentioned conventional cosmetics do not have a sufficient whitening effect, and also have problems in safety to the skin such as insufficient storage stability or irritation. There were many.

[0005]

SUMMARY OF THE INVENTION The present invention solves the above-mentioned problems of the prior art, and provides a whitening cosmetic having an excellent skin whitening effect, sufficient storage stability and high safety. The purpose is to do.

[0006]

Means for Solving the Problems The present inventors have conducted intensive studies in order to solve the above-mentioned problems. As a result, the extract of Hanatsuto, a kind of crude drug, inhibits tyrosinase enzyme activity and produces melanin in melanoma cells. The present invention has been found to have an inhibitory action, and the present invention can be provided.

[0007] That is, the present invention provides a skin-whitening cosmetic characterized by containing an extract of Hanabito, a crude drug.

[0008]

The cosmetic of the present invention can be manufactured by the following method. First, a crushed flower blood wisteria (also referred to as a large blood wisteria, a red wisteria, a large live blood, a large blood dwelling, a blood tree dwelling, a live blood wisteria, or the like) is extracted using an extraction solvent. In addition, as the extraction solvent, alcohol (methanol, ethanol, propanol, isopropyl alcohol, or the like), water, or the like can be used, and a mixed solution thereof can also be used. For example, if the alcohol concentration is
Extraction efficiency is good when extraction is performed at 50 ° C. for 1 hour using 20-70% aqueous alcohol.

After extraction, the extract is filtered to obtain an extract. The obtained extract is further concentrated under reduced pressure while heating at a temperature of 60 ° C. or lower to dryness, and the dried extract is collected and blended into cosmetics. In addition, you may mix the said extract in cosmetics as it is, fractionate the active ingredient which has a whitening effect, and mix it with cosmetics.

The present inventors have confirmed that the flower blood wisteria extract thus obtained exerts an excellent inhibitory action on melanin production at a low concentration as compared with conventionally used ascorbic acid. Have been. The amount of the flower blood wisteria extract to be added to the cosmetic is not particularly limited, but a whitening cosmetic having a whitening effect can be obtained by preferably adding 0.01 to 5.0%.

Hereinafter, the present invention will be described in more detail with reference to examples. However, the scope of the present invention is not limited by the following examples.

[0012]

[Embodiment 1] In this embodiment, an example of a method for extracting flower blood wisteria will be described. First, about 100 g of a dried product of Hanetsuto, a crude drug, was pulverized with a mixer, and the pulverized product and 500 ml of 50% ethyl alcohol were placed in a flask and refluxed at 50 ° C. for 1 hour with stirring. After the extraction, this solution was subjected to suction filtration, and the obtained filtrate was concentrated under reduced pressure at 50 ° C. using an evaporator. Next, the obtained concentrated liquid was dried under reduced pressure to obtain 11.9 g of brown crystals (extract).

[0013]

Example 2 In this example, the tyrosinase activity inhibitory effect of the extract of Hanatane wisteria obtained in Example 1 was measured. The tyrosinase activity inhibitory effect was measured by a method of quantifying dopachrome produced from dopa by tyrosinase by measuring absorbance at 475 nm. In measuring the tyrosinase activity inhibitory action, the following reaction reagents were used. (A) Sodium succinate buffer (100 mM, pH 5.
5) (b) Mushroom tyrosinase (manufactured by SIGMA) solution (prepared with the buffer (a) so as to be 270 units / ml) (c) L-DOPA (manufactured by Wako Pure Chemical Industries, Ltd.) solution (6 mM) First, prepare 1.8 ml of buffer (a) and (b) in a test tube.
0.1 ml of an aqueous solution prepared by adjusting the extract obtained in Example 1 to the final concentration shown in Table 1 was added to the test tube, and the mixture was incubated for 10 minutes in a constant temperature water bath at 30 ° C. . Next, the L-DOPA of (c) was added to this test tube.
After adding 1 ml of the solution and stirring, the test tube was set in a reciprocating shaker at about 45 ° in a constant temperature room at 30 ° C., and shaken for 40 minutes (reciprocation frequency 150 / min). After shaking, the absorbance at 475 nm was measured using a spectrophotometer, and the measured value was designated as A.

[0014]

[Table 1]

On the other hand, the absorbance at 475 nm was measured in the same manner as above except that the buffer (a) was added instead of the sample solution as a control. In addition, instead of the L-DOPA solution as a blank,
475 in the same manner as above except that buffer was added.
The absorbance at nm was measured, and the measured value was designated as C.

The tyrosinase activity inhibition rate of the sample solution was calculated from the measured value of the absorbance at 475 nm. The calculation of the tyrosinase activity inhibition rate is performed using the following formula,
The results are shown in Table 1.

Formula (1) Tyrosinase activity inhibition rate (%)
= {1− (A−C) / B} × 100 Based on the data in Table 1, the concentration of the flower vine extract that inhibits tyrosinase activity by 50% was 19.8 μg / ml. Even at low concentrations, the extract strongly inhibited tyrosinase activity and was confirmed to have excellent tyrosinase activity inhibitory activity.

[0018]

Example 3 In this example, the melanin production inhibitory effect of the flower blood wisteria extract obtained in Example 1 was measured.

First, B16 melanoma cells (B16-F0, ATCC), which are malignant melanoma cells derived from mice that produce melanin,
No. CRL-6322) was cultured in Eagle's MEM medium supplemented with fetal bovine serum to a final concentration of 10%, and the cells were added to each well of a 6-well plate (FALCON) at 3 × 10 5
⁶ ml of the above medium containing ³ cells / ml was added and cultured in a CO ₂ incubator (5% CO ₂ , 37 ° C.) for 5 days.

Next, the medium was added with 0.03% theophylline.
New Eagle MEM medium (6 ml) containing (SIGMA)
After adding an appropriate amount of a sample solution (aqueous solution of the extract obtained in Example 1) to each well, the cells were further cultured for 3 days. After completion of the culture, the medium was discarded, 1 ml of physiological saline was added to each well, and the cells adhered to the bottom of the well were suspended using a scraper to scrape the cells. Next, the cell suspension is transferred to a microcentrifuge tube using a pipette.
(1.5 ml volume, manufactured by Eppendorf) and centrifuged (1,000 × g, 15 minutes).

On the other hand, as a control, the same test as described above was conducted by adding sterilized water instead of the sample solution. In addition, as an experimental group for comparing the whitening of the cells, a 2% L-ascorbic acid aqueous solution was used instead of the sample solution (a) 60 μl, (b) 150 μl.
μl and (c) 300 μl were added, and the same test was performed.

Next, the degree of whitening of the pelleted cells was visually compared to determine the melanin production inhibitory effect. At that time, the degree of whiteness of the cells in the control experiment (sterilized water-added) was "-", and the degree of whiteness of the cells in the comparative experiment to which L-ascorbic acid was added was (a): "+", ( b)
: “++”, (c): As “++++”, the degree of whiteness of the cells when the sample solution was added was −, +, ++, +++
It was visually determined which one of the two corresponded, and the strength of the melanin production inhibitory effect of the sample solution was determined in four steps. The results are shown in Table 2.

[0023]

[Table 2]

[0024] As can be seen from Table 2, the extract of flower blood wisteria showed an inhibitory effect on melanogenesis equivalent to 200 µg / ml L-ascorbic acid at a concentration of 50 µg / ml, and at a lower concentration than L-ascorbic acid. It was confirmed that melanin production was suppressed. Each extract was not toxic to cells even at a high concentration of 800 μg / ml.

[0025]

Example 4 In this example, an example of blending the extract of Hanatane wisteria obtained in Example 1 with a whitening cosmetic is shown.

(% By weight) Flower blood wisteria extract 1.0 Glycerin 5.0 Polyoxyethylene sorbitan monolaurate 1.5 Ethanol 10.0 Appropriate amount Preservative, antioxidant Appropriate amount Pigment Appropriate amount Purified water balance

[0027]

EFFECTS OF THE INVENTION The extract of Hanetsuto, which is a crude drug, has an excellent inhibitory effect on tyrosinase activity and an inhibitory effect on melanin production in melanoma cells. Whitening cosmetics exhibit an excellent skin whitening effect. In addition, the extract of flower blood wisteria to be blended in the whitening cosmetic amount of the present invention exhibits excellent skin whitening effect in a small amount and has low toxicity to cells, so that it is highly safe.

──────────────────────────────────────────────────続 き Continuation of the front page (56) References JP-A-6-199885 (JP, A) (58) Fields investigated (Int. Cl. ⁷ , DB name) A61K ^7/ 00-7/50

Claims (1)

(57) [Claims] 1. A whitening cosmetic comprising an extract of a flower crude wisteria, which is a crude drug.