JP7455347B2 - tick attractant - Google Patents
tick attractant Download PDFInfo
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- JP7455347B2 JP7455347B2 JP2019106651A JP2019106651A JP7455347B2 JP 7455347 B2 JP7455347 B2 JP 7455347B2 JP 2019106651 A JP2019106651 A JP 2019106651A JP 2019106651 A JP2019106651 A JP 2019106651A JP 7455347 B2 JP7455347 B2 JP 7455347B2
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Description
本発明は、ダニを捕獲して死滅させる前段階において、まずは所定の場所にダニを誘引する効果を奏する製剤に関する。 The present invention relates to a preparation that is effective in attracting mites to a predetermined location before the mites are captured and killed.
従来、ダニは、適度な湿度及び温度が保たれている屋内に置かれている絨毯、カーペットなどの敷物や、布団、毛布、枕などの寝具、或いはセーター、コートなどの衣服や、ぬいぐるみなどの玩具などにおいて広く生息している。ダニの死骸やフンが粉砕されて生じた微粒子が空気中を舞って、人が吸い込んだり人に付着したりすることにより、アトピー性皮膚炎、気管支喘息、アレルギー性結膜炎、アレルギー性鼻炎などの種々のアレルギー反応を示すようになると言われている。 Traditionally, dust mites are found on carpets, carpets, bedding, futons, blankets, pillows, sweaters, coats, and stuffed animals that are kept indoors at a moderate humidity and temperature. It is widely used in toys, etc. When dust mite carcasses and feces are crushed into fine particles that fly through the air and are inhaled or attached to people, they can cause a variety of conditions such as atopic dermatitis, bronchial asthma, allergic conjunctivitis, and allergic rhinitis. It is said that some people may develop allergic reactions.
このようなダニに起因しアレルギー反応を引き起こすアレルゲンを発生させないために、ダニを駆除することが好ましいが、ダニは広い範囲で生息しているために、ダニの駆除剤を噴霧するなどの方法では効果が低く、高い効果を得るためには多量の駆除剤を使用する必要があり実用性に乏しい。このため、種々の箇所に生息しているダニを極力特定箇所に引き寄せて駆除するために、誘引剤が使用されている。 It is preferable to exterminate these mites in order to prevent them from producing allergens that cause allergic reactions, but since mites live in a wide range of areas, methods such as spraying with mite repellents are not recommended. The effectiveness is low, and in order to obtain a high effect, it is necessary to use a large amount of pesticide, making it impractical. For this reason, attractants are used to exterminate ticks that live in various locations by drawing them to a specific location as much as possible.
例えば、特許文献1において、炭素数が4から18の直鎖脂肪酸から選ばれた1種以上のか鉱物を有効成分としたダニ誘引組成物が開示され、実施例3にはそのうちオクタン酸が0.2g/m2となるように塗布された不織布シートにおいてダニ誘引効果が奏することが開示されている。
For example,
しかしながら、特許文献1のダニの誘引剤では、使用される脂肪酸が特有の臭気を有するために設置されている周囲では不快感を誘発することから、その使用量を少なくしながらも、高い誘引効果が求められているという課題があった。
However, in the tick attractant of
そこで、本発明では、製造が容易であり、ダニの誘引効果が高いダニ誘引剤を提供することを目的とする。 Therefore, an object of the present invention is to provide a mite attractant that is easy to manufacture and has a high mite attracting effect.
〔1〕すなわち、本発明は、炭素数6~18の脂肪酸と、ビール酵母又は米麹から選 ばれる少なくとも一種とを含有し、前記脂肪酸と、前記ビール酵母又は米麹から選ばれ る少なくとも一種の配合量の比は、(前記ビール酵母又は米麹から選ばれる少なくとも 一種)/(前記脂肪酸)=150~60000であることを特徴とするダニ誘引剤であ る。 [1] That is, the present invention contains a fatty acid having 6 to 18 carbon atoms and at least one selected from brewer's yeast or rice malt, and the fatty acid and at least one selected from brewer's yeast or rice malt. The mite attractant is characterized in that the blending ratio is (at least one selected from the above-mentioned brewer's yeast or rice malt )/(the above-mentioned fatty acid) = 150 to 60,000 .
〔2〕そして、前記脂肪酸が、カプロン酸、エナント酸、カプリル酸、ペラルゴン酸、 カプリン酸、ラウリン酸、ミリスチン酸、ペンタデシル酸、パルミチン酸、マルガリン 酸、ステアリン酸、オクチル酸、イソステアリン酸、パルミトレイン酸、オレイン酸、 リノール酸、リノレン酸から選ばれる少なくとも1種であることを特徴とする前記〔1 〕に記載のダニ誘引剤である。
[2] The fatty acid is caproic acid, enanthic acid, caprylic acid, pelargonic acid, capric acid, lauric acid, myristic acid, pentadecylic acid, palmitic acid, margaric acid, stearic acid, octylic acid, isostearic acid, palmitoleic acid. , oleic acid, linoleic acid, and linolenic acid, the tick attractant according to item [1] above .
本発明によれば、使用する脂肪酸の量を減らしてその特有の臭気による不快感を低減しながらも、ダニの高い誘引効果を奏する。すなわち、本発明によれば、炭素数6~18の脂肪酸、若しくは、発酵食品又は食品を発酵させる微生物の少なくとも一方をそれぞれ単独で使用した場合の効果の和よりも高い誘引効果が得られ、炭素数6~18の脂肪酸と、発酵食品又は食品を発酵させる微生物の少なくとも一方を包含することによる相乗効果が得られることから、ダニの誘引効果を奏しながらも、使用する脂肪酸の量を減らしてその特有の臭気による不快感を低減することができる。 According to the present invention, the amount of fatty acids used is reduced and the discomfort caused by the unique odor thereof is reduced, while a high mite-attracting effect is achieved. That is, according to the present invention, a higher attracting effect is obtained than the sum of the effects when at least one of fatty acids having 6 to 18 carbon atoms, fermented foods, or microorganisms that ferment foods are used alone, and carbon Since a synergistic effect can be obtained by including 6 to 18 fatty acids and at least one of fermented foods or microorganisms that ferment foods, it is possible to reduce the amount of fatty acids used and reduce the amount of fatty acids used, while still having the effect of attracting mites. It is possible to reduce discomfort caused by specific odors.
以下、本発明のダニ誘引剤に関する実施形態について詳しく説明する。なお、説明中における範囲を示す表記のある場合は、上限と下限を含有するものである。 EMBODIMENT OF THE INVENTION Hereinafter, embodiment regarding the tick attractant of this invention is described in detail. In addition, when there is a notation indicating a range in the description, it includes an upper limit and a lower limit.
本発明のダニ誘引剤に用いられる脂肪酸は、炭素数6~18の脂肪酸である。当該脂肪酸を単独で使用した場合にも、ダニに対しておおよそ一定の誘引効果を奏する。 The fatty acid used in the tick attractant of the present invention is a fatty acid having 6 to 18 carbon atoms. Even when the fatty acid is used alone, it has a roughly constant attracting effect on mites.
炭素数6~18の脂肪酸としては、このうち直鎖の脂肪酸、分岐鎖を有する脂肪酸や、飽和脂肪酸、不飽和脂肪酸を用いることができる。具体的には、直鎖の飽和脂肪酸としては、カプロン酸、エナント酸、カプリル酸、ペラルゴン酸、カプリン酸、ラウリン酸、ミリスチン酸、ペンタデシル酸、パルミチン酸、マルガリン酸、ステアリン酸が好ましい。そして、分岐鎖を有する飽和脂肪酸としては、オクチル酸、イソステアリン酸などが好ましい。そして、直鎖の不飽和脂肪酸としては、パルミトレイン酸、オレイン酸、リノール酸、リノレン酸などが好ましい。 Among these fatty acids having 6 to 18 carbon atoms, straight chain fatty acids, branched chain fatty acids, saturated fatty acids, and unsaturated fatty acids can be used. Specifically, as the linear saturated fatty acid, caproic acid, enanthic acid, caprylic acid, pelargonic acid, capric acid, lauric acid, myristic acid, pentadecyl acid, palmitic acid, margaric acid, and stearic acid are preferable. Preferred examples of the branched saturated fatty acid include octyl acid and isostearic acid. Preferred linear unsaturated fatty acids include palmitoleic acid, oleic acid, linoleic acid, and linolenic acid.
炭素数6~18の脂肪酸は、ダニ捕獲器などに設置される不織布やろ紙などの誘引剤の担持体に対して、0.0001~0.5g/m2となる量を塗工されることが好ましく、0.001~0.1g/m2となる量を塗工されることがさらに好ましい。炭素数6~18の脂肪酸が前記の量を塗工されると、発酵食品又は食品を発酵させる微生物の少なくとも一方と相乗的にダニを誘引することができる。 Fatty acids having 6 to 18 carbon atoms should be applied in an amount of 0.0001 to 0.5 g/m 2 to the attractant carrier such as nonwoven fabric or filter paper installed in tick traps, etc. is preferable, and it is more preferable to apply the coating in an amount of 0.001 to 0.1 g/m 2 . When the fatty acid having 6 to 18 carbon atoms is applied in the above amount, it can attract mites synergistically with at least one of the fermented food or the microorganism that ferments the food.
本発明のダニ誘引剤に用いられる発酵食品は、所定の食品が微生物により発酵されたものである。当該発酵食品を単独で使用した場合にも、ダニに対しておおよそ一定の誘引効果を奏する。 The fermented food used in the mite attractant of the present invention is a predetermined food fermented by microorganisms. Even when the fermented food is used alone, it has a roughly constant attracting effect on mites.
発酵食品としては、例えば、チーズ、バター、ヨーグルト、納豆、みそ、醤油、パンなどが好ましい。これらの発酵食品は、それぞれ単独で使用することもできるし、2種以上組み合わせて使用することもできる。 Preferred examples of fermented foods include cheese, butter, yogurt, natto, miso, soy sauce, and bread. These fermented foods can be used alone or in combination of two or more.
発酵食品は、ダニ捕獲器などに設置される不織布やろ紙などの誘引剤の担持体に対して、5~90g/m2となる量を塗工されることが好ましく、15~60g/m2となる量を塗工されることがさらに好ましい。発酵食品が前記の量を塗工されると、炭素数6~18の脂肪酸と相乗的にダニを誘引することができる。
The fermented food is preferably applied in an amount of 5 to 90 g/
本発明のダニ誘引剤に用いられる食品を発酵させる微生物は、カビや酵母などの菌類である。当該微生物を単独で使用した場合にも、ダニに対しておおよそ一定の誘引効果を奏する。 The microorganisms that ferment foods used in the mite attractant of the present invention are fungi such as molds and yeasts. Even when the microorganism is used alone, it has a roughly constant attracting effect on mites.
食品を発酵させる微生物としては、酵母菌、麹菌、乳酸菌、納豆菌などが好ましい。これらの微生物は、それぞれ単独で使用することもできるし、2種以上組み合わせて使用することもできる。 Preferred microorganisms for fermenting foods include yeast, koji mold, lactic acid bacteria, and natto bacteria. These microorganisms can be used alone or in combination of two or more.
食品を発酵させる微生物は、ダニ捕獲器などに設置される不織布やろ紙などの誘引剤の担持体に対して、5~110g/m2となる量を塗工されることが好ましく、15~90g/m2となる量を塗工されることがさらに好ましい。当該微生物が前記の量を塗工されると、炭素数6~18の脂肪酸と相乗的にダニを誘引することができる。 The microorganisms that ferment foods are preferably applied in an amount of 5 to 110 g/ m2 , and preferably 15 to 90 g, on an attractant carrier such as a nonwoven fabric or filter paper installed in a tick trap. It is more preferable to apply the coating in an amount of /m 2 . When the microorganism is applied in the above amount, it can attract mites synergistically with the fatty acid having 6 to 18 carbon atoms.
炭素数6~18の脂肪酸と、発酵食品又は食品を発酵させる微生物の少なくとも一方の配合量の比は、(発酵食品又は食品を発酵させる微生物の少なくとも一方)/(炭素数6~18の脂肪酸)=10~900000であることが好ましく、150~60000であることがさらに好ましい。配合量の比がこの範囲であると、使用する脂肪酸の量を減らしてその特有の臭気による不快感を低減しながらも、相乗的にダニを誘引することができるので実際に使用可能な誘引剤を製造することができる。 The ratio of the blending amount of a fatty acid having 6 to 18 carbon atoms and at least one of a fermented food or a microorganism that ferments the food is (at least one of the fermented food or a microorganism that ferments the food)/(fatty acid having 6 to 18 carbon atoms) = preferably from 10 to 900,000, more preferably from 150 to 60,000. When the blending ratio is within this range, it is possible to synergistically attract mites while reducing the amount of fatty acid used and reducing the discomfort caused by its unique odor, making it an actually usable attractant. can be manufactured.
さらに本発明のダニ誘引剤の酸化による劣化などの腐食を防止するため、ビタミンC(アスコルビン酸)、ビタミンE(α-トコフェロール)、BHT(ジブチルヒドロキシトルエン)、BHA(ブチルヒドロキシアニソール)、ソルビン酸、ソルビン酸カリウム、亜硫酸ナトリウムなどの酸化防止剤を添加することができる。また、防腐を目的に、パラオキシ安息香酸エステルを添加することもできる。 Furthermore, in order to prevent corrosion such as deterioration due to oxidation of the tick attractant of the present invention, vitamin C (ascorbic acid), vitamin E (α-tocopherol), BHT (dibutylhydroxytoluene), BHA (butylhydroxyanisole), sorbic acid, etc. Antioxidants such as potassium sorbate, sodium sulfite, etc. can be added. Moreover, paraoxybenzoic acid ester can also be added for the purpose of preservation.
〔実施例1〕
JIS L 1920「繊維製品の防ダニ性能試験方法」を参考にして、図1に示すようにしてダニの誘引性を試験した。すなわち、まず、直径約40mmのろ紙に、カプロン酸を0.001g/m2となる量、チーズを14.3g/m2となる量を塗工して検体1とし、直径約40mmの第1シャーレ2に敷き詰める。そして、ダニ培地3を第1シャーレ2よりも直径の大きい第2シャーレ4内に均一に広げ、10000匹相当分のダニを載置する。その第2シャーレ4の中央に第1シャーレ2を重ねて載置して、23~27℃の温度で、70~89%RHの湿度で、24時間静置した。その後、第2シャーレ4から第1シャーレ2内へのダニの侵入数を計測した。そして、脂肪酸、発酵食品、食品を発酵させる微生物のいずれも塗工しないろ紙を用いた以外は同様に試験を行い、第2シャーレ4から第1シャーレ2内へのダニの侵入数を計測し、コントロールとした(参考例0)。このコントロールにおける第1シャーレ内へのダニの侵入数に対する実施例1における第1シャーレ内へのダニの侵入数の比を効力比として算出した結果、5.15であった。なお、これらの試験は少なくとも3回以上行われ、上記結果は各試験結果の相加平均として示されている。
[Example 1]
With reference to JIS L 1920 "Testing method for mite-proof performance of textile products", the mite attraction was tested as shown in Figure 1. That is, first,
そして、カプロン酸とチーズについては、それぞれ実施例1と同様に試験を行い、それぞれの効力比を算出した。すなわち、ろ紙にカプロン酸を0.001g/m2となる量を塗工した以外は、実施例1と同様に試験を行い、コントロールに対する効力比を算出した結果、1.01であった(参考例1)。ろ紙にチーズを14.3g/m2となる量を塗工した以外は、実施例1と同様に試験を行い、コントロールに対する効力比を算出した結果、1.76であった(参考例11)。さらに、この参考例1の効力比と参考例11と効力比の和に対する実施例1の効力比の比を算出すると(実施例1の効力比/(参考例1の効力比+参考例11の効力比))、1.86となり、カプロン酸、チーズをそれぞれ単独に使用したときの和に対する比が1より有意に大きく、カプロン酸とチーズとの相乗効果があることがわかった。この結果を表1に示す。 Then, caproic acid and cheese were tested in the same manner as in Example 1, and their respective efficacy ratios were calculated. That is, the test was conducted in the same manner as in Example 1, except that the filter paper was coated with caproic acid in an amount of 0.001 g/m 2 , and the efficacy ratio relative to the control was calculated to be 1.01 (reference Example 1). The test was conducted in the same manner as in Example 1, except that the filter paper was coated with cheese in an amount of 14.3 g/m 2 , and the efficacy ratio relative to the control was calculated to be 1.76 (Reference Example 11) . Furthermore, when calculating the ratio of the efficacy ratio of Example 1 to the sum of the efficacy ratio of Reference Example 1 and the efficacy ratio of Reference Example 11, (Efficacy ratio of Example 1/(Efficacy ratio of Reference Example 1 + The efficacy ratio) was 1.86, and when caproic acid and cheese were used individually, the ratio to the sum was significantly greater than 1, indicating that there was a synergistic effect between caproic acid and cheese. The results are shown in Table 1.
〔実施例2~8〕
表1に示すように、各種脂肪酸とチーズを併用して、実施例1と同様に試験を行った。いずれの実施例においても、各種脂肪酸、チーズをそれぞれ単独に使用したときの和に対する比が1より有意に大きく、各種脂肪酸とチーズとの相乗効果があることがわかった。
[Examples 2 to 8]
As shown in Table 1, tests were conducted in the same manner as in Example 1 using various fatty acids and cheese in combination. In all Examples, when each of the various fatty acids and cheese were used individually, the ratio to the sum was significantly greater than 1, indicating that there was a synergistic effect between the various fatty acids and the cheese.
〔比較例1~2〕
表1に示すように、プロピオン酸又はアラキジン酸とチーズを併用して、実施例1と同様に試験を行った。いずれの実施例においても、プロピオン酸又はアラキジン酸、チーズをそれぞれ単独に使用したときの和に対する比が1と同程度であり、プロピオン酸又はアラキジン酸とチーズとの相乗効果がないことがわかった。
[Comparative Examples 1-2]
As shown in Table 1, a test was conducted in the same manner as in Example 1 using propionic acid or arachidic acid in combination with cheese. In all Examples, when propionic acid, arachidic acid, and cheese were used individually, the ratio to the sum was about the same as 1, and it was found that there was no synergistic effect between propionic acid, arachidic acid, and cheese. .
〔実施例9~16、比較例3~4〕
表2に示すように、各種脂肪酸とチーズを併用して、各種脂肪酸を0.01g/m2とした以外は実施例1と同様に試験を行った。いずれの実施例においても、各種脂肪酸、チーズをそれぞれ単独に使用したときの和に対する比が1より有意に大きく、各種脂肪酸とチーズとの相乗効果があることがわかった。また、比較例においては、プロピオン酸又はアラキジン酸、チーズをそれぞれ単独に使用したときの和に対する比が1未満であり、プロピオン酸又はアラキジン酸とチーズとの相乗効果がないことがわかった。
[Examples 9 to 16, Comparative Examples 3 to 4]
As shown in Table 2, the test was conducted in the same manner as in Example 1, except that various fatty acids and cheese were used in combination and the amount of various fatty acids was 0.01 g/m 2 . In all Examples, when each of the various fatty acids and cheese were used individually, the ratio to the sum was significantly greater than 1, indicating that there was a synergistic effect between the various fatty acids and the cheese. In addition, in the comparative example, when propionic acid or arachidic acid and cheese were used individually, the ratio to the sum was less than 1, and it was found that there was no synergistic effect between propionic acid or arachidic acid and cheese.
〔実施例17~24、比較例5~6〕
表3に示すように、各種脂肪酸とチーズを併用して、各種脂肪酸を0.1g/m2とした以外は実施例1と同様に試験を行った。いずれの実施例においても、各種脂肪酸、チーズをそれぞれ単独に使用したときの和に対する比が1より有意に大きく、各種脂肪酸とチーズとの相乗効果があることがわかった。また、比較例においては、プロピオン酸又はアラキジン酸、チーズをそれぞれ単独に使用したときの和に対する比が1と同程度であり、プロピオン酸又はアラキジン酸とチーズとの相乗効果がないことがわかった。
[Examples 17-24, Comparative Examples 5-6]
As shown in Table 3, the test was conducted in the same manner as in Example 1 except that various fatty acids and cheese were used in combination and the amount of various fatty acids was 0.1 g/m 2 . In all Examples, when each of the various fatty acids and cheese were used individually, the ratio to the sum was significantly greater than 1, indicating that there was a synergistic effect between the various fatty acids and the cheese. In addition, in the comparative example, when propionic acid, arachidic acid, and cheese were used individually, the ratio to the sum was about the same as 1, and it was found that there was no synergistic effect between propionic acid or arachidic acid and cheese. .
〔実施例25~48、比較例7~12〕
表4、表5、表6に示すように、各種脂肪酸をそれぞれ0.001g/m2、0.01g/m2、0.1g/m2とし、また、チーズに替えて所定量のバターを用いた以外は実施例1と同様に試験を行った。いずれの実施例においても、各種脂肪酸、バターをそれぞれ単独に使用したときの和に対する比が1より有意に大きく、各種脂肪酸とバターとの相乗効果があることがわかった。また、比較例においては、プロピオン酸又はアラキジン酸、バターをそれぞれ単独に使用したときの和に対する比が1未満又は1と同程度であり、プロピオン酸又はアラキジン酸とバターとの相乗効果がないことがわかった。
[Examples 25 to 48, Comparative Examples 7 to 12]
As shown in Tables 4, 5, and 6, various fatty acids were set at 0.001g/m 2 , 0.01g/m 2 , and 0.1g/m 2 , respectively, and a predetermined amount of butter was added in place of cheese. The test was conducted in the same manner as in Example 1, except that the sample was used. In all Examples, when each of the various fatty acids and butter were used individually, the ratio to the sum was significantly greater than 1, indicating that there was a synergistic effect between the various fatty acids and butter. In addition, in the comparative example, when propionic acid, arachidic acid, and butter are used individually, the ratio to the sum is less than 1 or about the same as 1, and there is no synergistic effect between propionic acid or arachidic acid and butter. I understand.
〔実施例49~72、比較例13~18〕
表7、表8、表9に示すように、各種脂肪酸をそれぞれ0.001g/m2、0.01g/m2、0.1g/m2とし、また、チーズに替えて所定量のビール酵母を用いた以外は実施例1と同様に試験を行った。いずれの実施例においても、各種脂肪酸、ビール酵母をそれぞれ単独に使用したときの和に対する比が1より有意に大きく、各種脂肪酸とビール酵母との相乗効果があることがわかった。また、比較例においては、プロピオン酸又はアラキジン酸、ビール酵母をそれぞれ単独に使用したときの和に対する比が1未満又は1と同程度であり、プロピオン酸又はアラキジン酸とビール酵母との相乗効果がないことがわかった。
[Examples 49 to 72, Comparative Examples 13 to 18]
As shown in Tables 7, 8, and 9, various fatty acids were set at 0.001g/m 2 , 0.01g/m 2 , and 0.1g/m 2 , respectively, and a predetermined amount of brewer's yeast was added instead of cheese. The test was conducted in the same manner as in Example 1 except that . In all Examples, when each of the various fatty acids and brewer's yeast were used individually, the ratio to the sum was significantly greater than 1, indicating that there was a synergistic effect between the various fatty acids and brewer's yeast. In addition, in the comparative examples, when propionic acid or arachidic acid and brewer's yeast are used individually, the ratio to the sum is less than 1 or about the same as 1, and the synergistic effect of propionic acid or arachidic acid and brewer's yeast is I found out that there isn't.
〔実施例73~96、比較例19~24〕
表10、表11、表12に示すように、各種脂肪酸をそれぞれ0.001g/m2、0.01g/m2、0.1g/m2とし、また、チーズに替えて所定量の米麹を用いた以外は実施例1と同様に試験を行った。いずれの実施例においても、各種脂肪酸、米麹をそれぞれ単独に使用したときの和に対する比が1より有意に大きく、各種脂肪酸と米麹との相乗効果があることがわかった。また、比較例においては、プロピオン酸又はアラキジン酸、米麹をそれぞれ単独に使用したときの和に対する比が1未満又は1と同程度であり、プロピオン酸又はアラキジン酸と米麹との相乗効果がないことがわかった。
[Examples 73-96, Comparative Examples 19-24]
As shown in Table 10, Table 11, and Table 12, various fatty acids were set at 0.001g/m 2 , 0.01g/m 2 , and 0.1g/m 2 , respectively, and a predetermined amount of rice malt was used instead of cheese. The test was conducted in the same manner as in Example 1 except that . In all Examples, when each of the various fatty acids and rice malt were used individually, the ratio to the sum was significantly greater than 1, indicating that there was a synergistic effect between the various fatty acids and rice malt. In addition, in the comparative example, when propionic acid, arachidic acid, and rice malt are used individually, the ratio to the sum is less than 1 or about the same as 1, and the synergistic effect of propionic acid or arachidic acid and rice malt is I found out that there isn't.
〔実施例97~120、比較例25~30〕
表13、表14、表15に示すように、各種脂肪酸をそれぞれ0.001g/m2、0.01g/m2、0.1g/m2とし、また、チーズを実施例1~24等よりもさらに増量して用いた以外は実施例1と同様に試験を行った。いずれの実施例においても、各種脂肪酸、チーズをそれぞれ単独に使用したときの和に対する比が1より有意に大きく、各種脂肪酸とチーズとの相乗効果があることがわかった。また、比較例においては、プロピオン酸又はアラキジン酸、チーズをそれぞれ単独に使用したときの和に対する比が1未満又は1と同程度であり、プロピオン酸又はアラキジン酸とチーズとの相乗効果がないことがわかった。
[Examples 97-120, Comparative Examples 25-30]
As shown in Tables 13, 14, and 15, various fatty acids were set at 0.001g/m 2 , 0.01g/m 2 , and 0.1g/m 2 , respectively, and cheese was selected from Examples 1 to 24, etc. The test was conducted in the same manner as in Example 1, except that the amount of the compound was further increased. In all Examples, when each of the various fatty acids and cheese were used individually, the ratio to the sum was significantly greater than 1, indicating that there was a synergistic effect between the various fatty acids and the cheese. In addition, in the comparative example, when propionic acid or arachidic acid and cheese are used individually, the ratio to the sum is less than 1 or about the same as 1, and there is no synergistic effect between propionic acid or arachidic acid and cheese. I understand.
〔実施例121~144、比較例31~36〕
表16、表17、表18に示すように、各種脂肪酸をそれぞれ0.001g/m2、0.01g/m2、0.1g/m2とし、また、チーズに替えて、実施例25~48等よりも多い量のバターを用いた以外は実施例1と同様に試験を行った。いずれの実施例においても、各種脂肪酸、バターをそれぞれ単独に使用したときの和に対する比が1より有意に大きく、各種脂肪酸とバターとの相乗効果があることがわかった。また、比較例においては、プロピオン酸又はアラキジン酸、バターをそれぞれ単独に使用したときの和に対する比が1未満又は1と同程度であり、プロピオン酸又はアラキジン酸とバターとの相乗効果がないことがわかった。
[Examples 121 to 144, Comparative Examples 31 to 36]
As shown in Table 16, Table 17, and Table 18, various fatty acids were set to 0.001 g/m 2 , 0.01 g/m 2 , and 0.1 g/m 2 , respectively, and cheese was replaced with Example 25 to The test was conducted in the same manner as in Example 1 except that a larger amount of butter than 48 etc. was used. In all Examples, when each of the various fatty acids and butter were used individually, the ratio to the sum was significantly greater than 1, indicating that there was a synergistic effect between the various fatty acids and butter. In addition, in the comparative example, when propionic acid, arachidic acid, and butter are used individually, the ratio to the sum is less than 1 or about the same as 1, and there is no synergistic effect between propionic acid or arachidic acid and butter. I understand.
〔実施例145~168、比較例37~42〕
表19、表20、表21に示すように、各種脂肪酸をそれぞれ0.001g/m2、0.01g/m2、0.1g/m2とし、また、チーズに替えて実施例49~72等よりも多い量のビール酵母を用いた以外は実施例1と同様に試験を行った。いずれの実施例においても、各種脂肪酸、ビール酵母をそれぞれ単独に使用したときの和に対する比が1より有意に大きく、各種脂肪酸とビール酵母との相乗効果があることがわかった。また、比較例においては、プロピオン酸又はアラキジン酸、ビール酵母をそれぞれ単独に使用したときの和に対する比が1未満又は1と同程度であり、プロピオン酸又はアラキジン酸とビール酵母との相乗効果がないことがわかった。
[Examples 145 to 168, Comparative Examples 37 to 42]
As shown in Table 19, Table 20, and Table 21, various fatty acids were set to 0.001 g/m 2 , 0.01 g/m 2 , and 0.1 g/m 2 , respectively, and cheese was replaced with Examples 49 to 72. The test was conducted in the same manner as in Example 1, except that a larger amount of brewer's yeast was used. In all Examples, when each of the various fatty acids and brewer's yeast were used individually, the ratio to the sum was significantly greater than 1, indicating that there was a synergistic effect between the various fatty acids and brewer's yeast. In addition, in the comparative examples, when propionic acid or arachidic acid and brewer's yeast are used individually, the ratio to the sum is less than 1 or about the same as 1, and the synergistic effect of propionic acid or arachidic acid and brewer's yeast is I found out that there isn't.
〔実施例169~192、比較例43~48〕
表22、表23、表24に示すように、各種脂肪酸をそれぞれ0.001g/m2、0.01g/m2、0.1g/m2とし、また、チーズに替えて実施例73~96等よりも多い量の米麹を用いた以外は実施例1と同様に試験を行った。いずれの実施例においても、各種脂肪酸、米麹をそれぞれ単独に使用したときの和に対する比が1より有意に大きく、各種脂肪酸と米麹との相乗効果があることがわかった。また、比較例においては、プロピオン酸又はアラキジン酸、米麹をそれぞれ単独に使用したときの和に対する比が1未満又は1と同程度であり、プロピオン酸又はアラキジン酸と米麹との相乗効果がないことがわかった。
[Examples 169-192, Comparative Examples 43-48]
As shown in Table 22, Table 23, and Table 24, various fatty acids were set to 0.001 g/m 2 , 0.01 g/m 2 , and 0.1 g/m 2 , respectively, and cheese was replaced with Examples 73 to 96. The test was conducted in the same manner as in Example 1, except that a larger amount of rice malt was used than in Example 1. In all Examples, when each of the various fatty acids and rice malt were used individually, the ratio to the sum was significantly greater than 1, indicating that there was a synergistic effect between the various fatty acids and rice malt. In addition, in the comparative example, when propionic acid, arachidic acid, and rice malt are used individually, the ratio to the sum is less than 1 or about the same as 1, and the synergistic effect of propionic acid or arachidic acid and rice malt is I found out that there isn't.
以上の結果より、各炭素数6~18の各種脂肪酸であるカプロン酸、カプリル酸、ペラルゴン酸、カプリン酸、パルミチン酸、ステアリン酸、オレイン酸、リノール酸と、発酵食品であるチーズ、バター又は食品を発酵させる微生物であるビール酵母、米麹を併用することにより、それぞれ使用した場合の効果の和よりも高い相乗的な誘引効果が得られることがわかった。 From the above results, we found that caproic acid, caprylic acid, pelargonic acid, capric acid, palmitic acid, stearic acid, oleic acid, and linoleic acid, which are various fatty acids with 6 to 18 carbon atoms, and fermented foods such as cheese, butter, and foods. It was found that by using beer yeast and rice koji, which are microorganisms that ferment , in combination, a synergistic attracting effect greater than the sum of the effects of each was obtained.
1・・・検体
2・・・第1シャーレ
3・・・ダニ培地
4・・・第2シャーレ
1...
Claims (2)
前記脂肪酸と、前記ビール酵母又は米麹から選ばれる少なくとも一種の配合量の比は、 (前記ビール酵母又は米麹から選ばれる少なくとも一種)/(前記脂肪酸)=150~ 60000である
ことを特徴とするダニ誘引剤。 Contains a fatty acid having 6 to 18 carbon atoms and at least one selected from brewer's yeast or rice malt,
The ratio of the amount of the fatty acid and at least one selected from the brewer's yeast or rice malt is (at least one selected from the beer yeast or rice malt)/(the fatty acid) = 150 to 60,000.
A tick attractant characterized by:
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