JP7489153B1 - Method for measuring components contained in aerosol, measuring device, reaction solution and mixing kit thereof - Google Patents

Abstract

[Problem] To easily and accurately measure components contained in aerosol in the atmosphere. [Solution] A method for measuring a target component contained in aerosol in the atmosphere, comprising the steps of: (a) preparing a reaction liquid containing a first substance that reacts with the target component; (b) taking in an aerosol into the reaction liquid, reacting the target component with the first substance, and generating a second substance; (c) oxidizing or reducing the second substance in the presence of a third substance; and (d) evaluating the presence or absence of the target component in the aerosol based on the current value generated by returning the third substance, which has changed by contributing to the oxidation or reduction of the second substance, to its original state by an electrochemical method. [Selected Figure] Figure 1

Description

The present invention relates to a method for measuring the components contained in an aerosol, a measuring device, a reaction liquid, and a mixing kit thereof.

For example, in poorly ventilated indoor environments, people crowded together can lead to a poor environmental atmosphere. This is because saliva from people's breath and fine particles such as dust (e.g. PM2.5) and pollen brought in as people move around are suspended in the environmental atmosphere. This saliva and fine particles exist in the environmental atmosphere as aerosols. If saliva contains viruses or other pathogens, it can cause infection via aerosols. Fine particles can cause respiratory diseases, etc.

Therefore, there is a demand for measuring the components contained in aerosols in the environmental atmosphere. For example, Patent Document 1 proposes a method for this purpose in which a filter is used to capture aerosols contained in the air, and then the components captured by the filter are irradiated with excitation light and the luminescence of the captured components is measured.

JP 2022-080329 A

However, with the measurement method of Patent Document 1, even if the components contained in the aerosol are collected with a filter, it may not be possible to selectively measure a specific component contained therein. In addition, since a device for irradiating excitation light and a device for observing the emitted light are required, it may not be possible to easily measure the components contained therein.

The present invention was made in consideration of the above problems, and aims to provide a technology for easily and selectively measuring the components contained in aerosols in the atmosphere.

One aspect of the present invention is
A method for measuring a target component contained in an aerosol in an atmosphere, comprising:
(a) preparing a reaction solution containing a first substance that reacts with the target component;
(b) taking in the aerosol in the reaction liquid, reacting the target component with the first substance, and generating a second substance;
(c) oxidizing or reducing the second substance in the presence of a third substance;
(d) evaluating the presence or absence of the target component in the aerosol based on a current value generated by returning the third substance, which has been changed by contributing to the oxidation or reduction of the second substance, to its original state by an electrochemical method;
This is a method for measuring the components contained in aerosols.

Another aspect of the present invention is
An apparatus for measuring a target component contained in an aerosol in the atmosphere, comprising:
A reaction unit configured to accommodate a reaction liquid containing a first substance that reacts with the target component and to incorporate the aerosol into the reaction liquid;
A measurement unit that measures the reaction solution by an electrochemical method;
A determination unit that determines the presence or absence of the target component in the aerosol based on a result from the measurement unit,
In the reaction section, when the aerosol is taken into the reaction liquid, a second substance is generated by a reaction between the target component and the first substance, and the second substance is oxidized or reduced in the presence of a third substance;
The measurement unit measures a current value generated by returning the third substance, which has changed by contributing to the oxidation or reduction of the second substance in the reaction solution, to its original state by an electrochemical method.
This is a device for measuring the components contained in aerosols.

Yet another aspect of the present invention is
A reaction solution for measuring amylase contained in an aerosol, comprising:
At least one of amylose and amylopectin, at least one of glucose oxidase and glucose dehydrogenase, and an electron mediator.
This is a reaction solution for amylase measurement.

Yet another aspect of the present invention is
A mixing kit for preparing a reaction solution for measuring amylase contained in an aerosol, comprising:
At least one of amylose and amylopectin;
at least one of glucose oxidase and glucose dehydrogenase;
and an electron mediator, which are contained separately and are configured to be prepared as the reaction liquid by mixing.
This is a reaction mixture kit for amylase measurement.

Yet another aspect of the present invention is
A reaction solution for measuring pathogens contained in aerosol,
the pathogen is at least one of an influenza virus and a Streptococcus pneumoniae having a neuraminidase in its chemical structure;
The present invention relates to a method for producing a lactase comprising the steps of: (a) extracting lactose from a lactose extract; (b) extracting lactose from a lactose extract; and (c) extracting lactose from a lactose extract.
This is a reaction solution for measuring pathogens.

Yet another aspect of the present invention is
A mixing kit for preparing a reaction solution for measuring pathogens contained in aerosol, comprising:
the pathogen is at least one of an influenza virus and a Streptococcus pneumoniae having a neuraminidase in its chemical structure;
The reaction solution is prepared by mixing sialyllactose, lactase, at least one of glucose oxidase and glucose dehydrogenase, and an electron mediator, which are contained separately.
This is a mixed reaction solution kit for measuring pathogens.

The present invention makes it possible to easily and accurately measure the components contained in aerosols in the atmosphere.

FIG. 1 is a schematic diagram of a measurement device according to an embodiment of the present invention. FIG. 2 is a flow diagram of a measurement method according to one embodiment of the present invention.

<One embodiment of the present invention>
An embodiment of the present invention will be described below. Prior to describing the measurement method and measurement device of this embodiment, the reaction solution used therein will be described below.

(1) Reaction liquid The reaction liquid of this embodiment is used to measure components contained in aerosols in the environmental atmosphere. The reaction liquid is used to take in aerosols in the atmosphere, collect the components contained in the aerosols, and react the components so that they can be measured by an electrochemical method described below. Here, the components to be measured (target components) are components that form aerosols and float in the air, such as amylase derived from saliva and pathogens such as viruses.

Specifically, the reaction liquid contains at least a first substance that reacts with the target component and a solvent. In addition to these components, the reaction liquid may contain other additives as necessary. Each component will be described in detail below.

(First substance)
The first substance is a compound that reacts with the target component in the aerosol to generate a second substance that can be measured by an electrochemical method. The first substance can be appropriately changed depending on the target component. For example, when the target component is amylase derived from saliva, it is preferable to use at least one of amylose and amylopectin as the first substance. Amylose and amylopectin are hydrolyzed by reacting with amylase in saliva to generate glucose as the second substance. Also, for example, when the target component is a component having neuraminidase in its chemical structure (such as influenza virus or Streptococcus pneumoniae), it is preferable to use sialyllactose and lactase as the first substance.

The content of the first substance in the reaction solution is not particularly limited, but it is preferable that the content be in excess of the target component. The presence of an excess amount of the first substance in the reaction solution allows the target component and the first substance to react more reliably. In addition, when the target component is amylase and the first substance is amylose or amylopectin, the amount of glucose produced can be increased by repeatedly hydrolyzing the excess amount of amylose, etc. with amylase, and the sensitivity can be increased when measuring using the electrochemical method described below.

(solvent)
The solvent is not particularly limited as long as it can capture and collect the aerosol and dissolve the first substance, and for example, water can be used. It is preferable to use a pH buffer solution having a buffering effect. The pH range may be appropriately adjusted depending on the type of the first substance and the second substance to be reacted.

(Third substance)
The reaction solution preferably contains a third substance that oxidizes or reduces the second substance. The third substance is an oxidizing agent capable of oxidizing the second substance or a reducing agent capable of reducing the second substance. For example, when an oxidizing agent is used as the third substance, the second substance is oxidized by the third substance to become an oxidized form, while the third substance is reduced to become a reduced form by receiving electrons as the second substance is oxidized. Conversely, when a reducing agent is used as the third substance, the second substance is reduced by the third substance to become a reduced form, while the third substance is oxidized to become an oxidized form. In this embodiment, as will be described in detail later, an electrochemical current response is detected when the third substance in a reduced or oxidized form is returned to its original state, that is, when it is oxidized or reduced. This response corresponds to the content of the oxidizing agent in the reduced form. The content of the oxidizing agent in the reduced form corresponds to the amount of glucose produced, and the amount of glucose produced is an indicator of the content of amylase in the aerosol taken in.

The third substance can be changed as appropriate depending on the type of the second substance. When the second substance is glucose, it is preferable to use an oxidizing agent as the third substance, and more specifically, it is more preferable to use at least one of glucose oxidase and glucose dehydrogenase. The content of the third substance is not particularly limited as long as it is an amount that can oxidize or reduce a sufficient amount of the second substance produced, and may be changed as appropriate depending on the amount of the second substance produced.

(Electron Mediator)
The reaction solution preferably further contains an electron mediator. The electron mediator acts to mediate the redox reaction during measurement by an electrochemical method. Specifically, the electron mediator can promote the transfer of electrons between the third substance in a reduced or oxidized form and the electrode of the measurement device. The electron mediator allows for more accurate detection of the response that occurs when the third substance that contributed to the redox of the second substance returns to its original state.

As the electron mediator, at least one of common electron mediators such as potassium ferricyanide, p-benzoquinone, p-benzoquinone derivatives, oxidized phenazine methosulfate, methylene blue, ferrocene and ferrocene derivatives, dichloroindophenol, and 1,10-phenanthroline-5,6-dione can be used.

The amount of electron mediator contained is not particularly limited, but from the viewpoint of detecting the response more accurately when measuring by electrochemical techniques, it is preferable to use an amount in excess of the amount of the target component.

(2) Measuring Device Next, a measuring device for measuring components contained in an aerosol will be described with reference to Fig. 1. Fig. 1 is a schematic diagram of a measuring device according to one embodiment of the present invention.

The measurement device 1 of this embodiment includes a reaction unit 10 that contains a reaction liquid S, a measurement unit 20 that measures the reaction liquid S by an electrochemical method, and a determination unit 30 that evaluates the presence or absence of contained components based on the measurement results.

(Reaction Section)
The reaction unit 10 is configured to accommodate a reaction liquid S, and to take in an aerosol from the ambient atmosphere into the reaction liquid S, and to react the target component in the reaction liquid S. For example, a measurement cell having an opening at the top can be used as the reaction unit 10.

In the reaction section 10, when an aerosol is taken into the reaction liquid S, if the aerosol contains a target component, the following reaction occurs. First, a first substance reacts with the target component in the reaction liquid S to produce a second substance. Next, the second substance is oxidized or reduced by a third substance. Meanwhile, the third substance contributes to the oxidation or reduction of the second substance, and is thereby reduced or oxidized.

(Measuring unit)
The measurement unit 20 performs measurements on the reaction solution S by an electrochemical method. The measurement unit 20 is configured to perform, for example, voltammetry measurements. Specifically, the measurement unit 20 includes a working electrode 21, a counter electrode 22, and a reference electrode 23 as electrodes, a potential sweep unit 24, and a current measurement unit 25.

Each electrode is positioned so that it is immersed in the reaction solution S in the reaction section 10. During measurement, the working electrode 21 oxidizes or reduces a third substance that has become reduced or oxidized in the reaction solution S, returning it to its original state. If an electron mediator is contained in the reaction solution S, the third substance is reduced or oxidized via the electron mediator. The counter electrode 22 causes a reverse reaction with the working electrode 21. The reference electrode 23 serves as a standard for maintaining a constant potential relative to the working electrode 21.

As each electrode, there is no particular limitation as long as it can measure the reduction current, and conventionally known electrodes can be used. As the working electrode 21 and the counter electrode 22, for example, a metal electrode such as gold or platinum, or a carbon electrode can be used. As the reference electrode 23, for example, a silver/silver chloride electrode can be used.

The potential sweep unit 24 is connected to each electrode and configured to sweep the voltage applied to the working electrode 21 as a potential relative to the reference electrode 23, for example, within a predetermined range and at a predetermined sweep speed. Specifically, the potential sweep unit 24 sweeps the potential of the working electrode 21 positively when a third substance in a reduced form is to be returned to its original state by oxidation, and conversely, sweeps the potential of the working electrode 21 negatively when a third substance in an oxidized form is to be returned to its original state by reduction.

The potential sweep unit 24 only needs to be able to adjust the potential at the working electrode 21 to a level that returns the third substance in a reduced or oxidized form to its original state, and may be changed as appropriate depending on the type of third substance.

The potential sweep unit 24 is not particularly limited in its sweep method as long as it can measure the current response associated with redox, and it may be changed as appropriate depending on the measurement method, such as cyclic voltammetry or linear voltammetry.

The current measuring unit 25 is connected to each electrode and configured to measure the current response flowing between the working electrode 21 and the counter electrode 22 when the potential is swept. For example, when the third substance in the reaction solution S is in a reduced form, the current measuring unit 25 obtains the oxidation current value when the third substance returns from the reduced form to the original state, and when the third substance is in an oxidized form, the current measuring unit 25 obtains the reduction current value when the third substance returns from the oxidized form to the original state.

(Determination unit)
The determination unit 30 is connected to the measurement unit 20 and configured to determine the presence or absence of a target component contained in the reaction solution S based on the result from the measurement unit 20, and is configured, for example, with a determination processing unit 31. For example, if a current response is detected by the measurement unit 20, the determination processing unit 31 determines that the target component has been taken into the reaction solution S, that is, that the target component is present in the environmental atmosphere. On the other hand, if no current response is detected by the measurement unit 20, it determines that the target component is not present in the environmental atmosphere.

The determination unit 30 is preferably configured so that the determination processing unit 31 can quantify the amylase in the aerosol taken into the reaction solution S based on the magnitude of the current value acquired by the measurement unit 20. It is more preferable that the determination unit 30 further includes, in addition to the determination processing unit 31, a memory unit 32 that stores a calibration curve showing the correlation between the current value and the aerosol content. In this case, the determination processing unit 31 can quantify the amylase content in the reaction solution S from the calibration curve based on the current value.

(Control Unit)
The control unit 40 is, for example, equipped with a computer, and is configured to be connected to the measurement unit 20 and the judgment unit 30 and control them. Specifically, in the measurement unit 20, the potential sweep unit 24 is controlled so as to sweep the voltage applied to the working electrode 21 as the potential relative to the reference electrode 23 within a predetermined range, and the current measurement unit 25 is controlled so as to measure the current flowing between the working electrode 21 and the counter electrode 22 during the potential sweep. In addition, in the judgment unit 30, the judgment processing unit 31 is controlled so as to judge the presence or absence of the target component contained in the reaction solution S from the current value acquired by the measurement unit 20. In addition, when the judgment unit 30 includes a memory unit 32, the judgment processing unit 31 is controlled so as to quantify the content of the target component contained in the reaction solution S by referring to the calibration curve in the memory unit 32.

(3) Method for measuring components contained in aerosol Next, a method for measuring a target component contained in aerosol in the atmosphere will be described with reference to Fig. 2. Fig. 2 is a flow diagram of a measurement method according to one embodiment of the present invention. Below, a case where amylase is measured as a target component will be described as an example.

(Preparation step S1)
First, a reaction solution S for amylase measurement is prepared. For example, amylose that reacts with the target component amylase as a first substance, water as a solvent, preferably a pH buffer solution, glucose oxidase that can oxidize glucose generated by hydrolysis of amylose in the reaction with amylase as a third substance, and potassium ferricyanide as an electron mediator are mixed to prepare the reaction solution S. This reaction solution S is placed in the reaction unit 10.

(Incorporation step S2)
Next, the aerosol is taken into the reaction liquid S. At this time, the following reaction occurs depending on the presence or absence of amylase in the aerosol.

If the aerosol contains amylase, the amylose and amylopectin in reaction solution S are hydrolyzed by reacting with the amylase to produce the second substance, glucose. The glucose produced is oxidized by the oxidizing agent in reaction solution S. The oxidizing agent is reduced by contributing to the oxidation of glucose. On the other hand, if the aerosol does not contain amylase, the production of glucose does not proceed, and the amylose and amylopectin remain as they are.

The method of incorporating the aerosol into the reaction liquid S is not particularly limited, but it is preferable to arrange the reaction liquid S so that it is exposed to the ambient atmosphere. In this case, for example, the reaction liquid S may be placed in the reaction section 10 and left exposed for a predetermined period of time. This allows the aerosol to be incorporated into the reaction liquid S, and the second substance to be generated in the reaction liquid S. Note that, in order to promote the incorporation of the aerosol, air from the ambient atmosphere may be blown into the reaction liquid S.

The time for incorporating the aerosol into the reaction solution S is not particularly limited, but it is preferable to make the incorporation time longer. The longer the incorporation time, the more reliably the aerosol in the atmosphere can be incorporated into the reaction solution S, and the amount of the target component, amylase, incorporated can be increased. The incorporation time is also the time for reacting amylase with amylose (hereinafter also referred to as the reaction time), and by extending this time, amylose and amylopectin can be repeatedly hydrolyzed in the reaction solution S, and the amount of glucose produced can be increased. By increasing the amount of glucose produced, the amount of oxidizing agent contributing to the oxidation of glucose can be increased. This allows measurement with high sensitivity in the measurement step S3 described later. The incorporation time (reaction time) is not particularly limited, but it is preferable to set it to a time such that the amount of glucose produced is greater than the amount of amylase incorporated into the reaction solution S. The incorporation time corresponds to the exposure time from when the reaction solution S begins to be exposed to the environmental atmosphere until it is subjected to the measurement step S3 described later.

(Measurement step S3)
Next, the reaction solution S is measured by an electrochemical method in the measurement unit 20. Specifically, the working electrode 21, the counter electrode 22, and the reference electrode 23 are immersed in the reaction solution S, and a positive voltage is applied to the working electrode 21 by the potential sweep unit 24. As a result, the oxidizing agent in a reduced form at the working electrode 21 is oxidized and returns to its original state. At this time, the electron mediator mediates the transfer of electrons between the oxidizing agent in a reduced state and the working electrode 21. The current measurement unit 25 measures the value of the oxidation current flowing between the working electrode 21 and the counter electrode 22 in association with the oxidation reaction occurring at the working electrode 21 by potential sweeping.

The current value obtained in the measurement step S3 is an index showing the amount of amylase contained in the aerosol taken into the reaction solution S per unit time.

(Determination step S4)
Next, the determination processing section 31 of the determination section 30 determines the presence or absence of amylase taken into the reaction solution S based on the measurement results from the measurement section 20, specifically, the oxidation current value acquired by the current measurement section 25. For example, if an oxidation current value is detected, it is determined that amylase has been taken into the reaction solution S and that amylase is present in the environmental atmosphere. On the other hand, if an oxidation current value is not detected, it is determined that amylase is not present in the environmental atmosphere.

In the judgment step S4, it is preferable to set a threshold value for the oxidation current value and judge whether the environmental atmosphere is good or bad based on the threshold value. By judging based on the threshold value, it is possible to judge the quality of the environmental atmosphere with a margin of error.

In this way, it is possible to measure whether or not amylase is contained in the aerosol in the environmental atmosphere. In addition, by repeating the above-mentioned series of steps at intervals, it is possible to measure changes over time in the quality of the environmental atmosphere.

<Effects of this embodiment>
According to this embodiment, one or more of the following advantages are achieved.

(a) According to the measurement method of this embodiment, first, aerosol floating in the environmental atmosphere is taken into the reaction solution S. As a result, when amylase is contained in the aerosol, the amylase can be captured in the reaction solution S. The captured amylase hydrolyzes amylose and the like in the reaction solution S to generate glucose. This glucose is oxidized in the presence of an oxidizing agent, and the oxidizing agent contributes to the oxidation and becomes a reduced form. Then, an electrochemical method is used to measure the oxidation current value generated when the reduced oxidizing agent is oxidized to return to its original state. This oxidation current value is a value corresponding to the amount of oxidizing agent that contributed to the oxidation of glucose, and a value corresponding to the amount of amylase that contributed to the generation of glucose. Therefore, based on the oxidation current value, it is possible to selectively and easily measure whether or not amylase is contained in the aerosol in the environmental atmosphere.

(b) It is preferable to contain the reaction liquid S in the reaction section 10 and to arrange the reaction liquid S so that it is exposed to the environmental atmosphere. This makes it possible to measure the components contained in the aerosol suspended in the environmental atmosphere simply by leaving the reaction liquid S stationary in the environmental atmosphere. In other words, it is possible to more easily evaluate the environmental atmosphere.

(c) It is preferable that the reaction solution S further contains an electron mediator, and that the measurement of the oxidation current value is carried out in the presence of the electron mediator. The electron mediator promotes the transfer of electrons between the oxidized oxide and the working electrode 21, and allows for more accurate measurement of the oxidation current value that flows between the electrodes in association with the oxidation reaction at the working electrode 21.

(d) It is preferable that the reaction solution S contains amylose and amylopectin in excess of the amount of amylase taken up. This allows the amylase to repeatedly hydrolyze amylose and the like, thereby increasing the amount of glucose produced. As a result, the sensitivity is increased in the measurement step S3, allowing for accurate measurements.

(e) It is preferable that the reaction solution S contains an excess amount of the electron mediator relative to the amount of amylase to be taken up. This can promote the transfer of electrons by the electron mediator in the measurement step S3, and can more accurately measure the oxidation current value flowing between the electrodes in association with the oxidation reaction at the working electrode 21.

(f) The reaction time from the uptake step S2 to the measurement step S3 is preferably set so that the amount of glucose produced is greater than the amount of amylase taken up. If the amount of amylase contained in the aerosol is extremely small, if the reaction time is too short, the amount of glucose produced will also be extremely small, and measurement with high sensitivity in the measurement step S3 may not be possible. In this regard, by extending the reaction time so that the amount of glucose produced is greater than the amount of amylase taken up, the current response in the measurement step S3 can be amplified and measurement can be performed with high sensitivity.

<Other embodiments>
Although an embodiment of the present invention has been described above, the present invention is not limited to the above-described embodiment, and various modifications can be made without departing from the spirit of the present invention.

In the above embodiment, an oxidizing agent is added to the reaction solution S, and amylase and amylose are reacted to generate glucose, and the glucose is oxidized at the same time, but the present invention is not limited to this. For example, an oxidizing agent may not be added to the reaction solution S in advance, and the oxidizing agent may be added after the aerosol is taken into the reaction solution S and before measurement is performed by an electrochemical method.

In the above embodiment, the electron mediator is added to the reaction solution S in advance, but the present invention is not limited to this. The electron mediator only needs to be present in the reaction solution S during the measurement step S3, and may be added to the reaction solution S after the capture step S2 and before the measurement step S3 is performed, for example.

In the above embodiment, the measurement unit 20 is described as being of a three-electrode type, but the present invention is not limited to this. The measurement unit 20 may be of a two-electrode type, and may be configured to include, for example, a working electrode and a counter electrode that also serves as a reference electrode.

In the above embodiment, the reaction solution S, which is a mixture of at least one substrate of amylose and amylopectin, at least one oxidizing agent of glucose oxidase and glucose dehydrogenase, and an electron mediator, is stored in the reaction unit 10, but the present invention is not limited to this. When the substrate, oxidizing agent, and electron mediator are mixed, the reaction solution S may deteriorate due to the reaction between them. In this regard, it is preferable to use a mixed kit of the reaction solution S, in which the substrate, oxidizing agent, and electron mediator are stored separately. The mixed kit is configured, for example, by storing a solution containing a substrate, a solution containing an oxidizing agent, and a solution containing an electron mediator in separate containers. With this mixed kit, the reaction solution S can be prepared by supplying and mixing each solution from each container at any time. The mixed kit can be stored in the reaction unit 10 as it is, and the reaction solution S can be prepared by mixing them at the start of the measurement. This makes it possible to suppress deterioration of the reaction solution S before the measurement.

In the above embodiment, a case has been described in which amylase derived from saliva is measured as the target component, but the present invention is not limited to this. The target component can also be a component that has neuraminidase in its chemical structure. Examples of such components include pathogens such as influenza viruses and Streptococcus pneumoniae that have neuraminidase on their surface. Methods for measuring these components are described below.

First, prepare a reaction solution for measuring pathogens. This reaction solution can be prepared by mixing a solvent, the first substances sialyllactose and lactase, the third substance glucose oxidase, and the electron mediator potassium ferricyanide.

The reaction liquid is placed so as to be exposed to the ambient atmosphere. This allows the aerosol present in the ambient atmosphere to be incorporated into the reaction liquid. At this time, if influenza virus is present in the aerosol, neuraminidase present on the surface of the influenza virus reacts with sialyllactose in the reaction liquid to produce lactose. This lactose produces glucose and galactose by lactase in the reaction liquid. Then, as in the above-mentioned embodiment, glucose oxidase contributes to the oxidation of glucose and is reduced, and the oxidation current value generated when this reduced glucose oxidase is returned to its original state is measured. Based on this current value, it is possible to determine whether influenza virus has been incorporated into the reaction liquid, that is, the presence or absence of influenza virus in the ambient atmosphere. In this way, compounds containing neuraminidase in the chemical structure of influenza virus, etc. can be selectively and easily measured.

The pathogen measurement reaction solution may be in a premixed state, as with the amylase measurement reaction solution, or each component may be housed separately to form a mixed kit. For example, a mixed kit may be configured such that a solution containing sialyllactose, a solution containing lactase, a solution containing at least one of glucose oxidase and glucose dehydrogenase, and a solution containing an electron mediator are each housed separately and are mixed to prepare the above reaction solution. This makes it possible to suppress deterioration of the reaction solution in a mixed state.

<Preferred embodiment of the present disclosure>
Preferred aspects of the present disclosure are described below.

(Appendix 1)
A method for measuring a target component contained in an aerosol in an atmosphere, comprising:
(a) preparing a reaction solution containing a first substance that reacts with the target component;
(b) taking in the aerosol in the reaction liquid, reacting the target component with the first substance, and generating a second substance;
(c) oxidizing or reducing the second substance in the presence of a third substance;
(d) evaluating the presence or absence of the target component in the aerosol based on a current value generated by returning the third substance, which has been changed by contributing to the oxidation or reduction of the second substance, to its original state by an electrochemical method;
A method for measuring the components contained in aerosols.

(Appendix 2)
the target component is amylase derived from saliva contained in the aerosol,
the first substance is at least one of amylose and amylopectin;
In the step (b), an aerosol containing the amylase is introduced into the reaction liquid, and the first substance is hydrolyzed by the amylase to generate glucose as the second substance;
In the step (c), the glucose is oxidized in the presence of an oxidizing agent as the third substance;
The step (d) includes a step of determining the presence or absence of the amylase in the aerosol based on a current value generated by returning the oxidizing agent, which has been changed by contributing to the oxidation of the glucose, to its original state by an electrochemical method.
A method for measuring the components contained in the aerosol described in Appendix 1.

(Appendix 3)
the target component is a component having neuraminidase in its chemical structure,
the first substance is sialyllactose and lactase;
In the step (b), an aerosol containing a component having the neuraminidase is introduced into the reaction liquid, the neuraminidase is hydrolyzed to lactose by the sialyllactose, and glucose is produced as the second substance from the lactose by the lactase;
In the step (c), the glucose is oxidized in the presence of an oxidizing agent as the third substance;
In the step (d), the presence or absence of the amylase in the aerosol is determined based on a current value generated by returning the oxidizing agent, which has been changed by contributing to the oxidation of the glucose, to its original state by an electrochemical method.
A method for measuring the components contained in the aerosol described in Appendix 1.

(Appendix 4)
The component having neuraminidase is at least one of influenza virus and Streptococcus pneumoniae.
A method for measuring the components contained in the aerosol described in Appendix 3.

(Appendix 5)
In the step (b), the reaction liquid is placed so as to be exposed to the ambient atmosphere.
A method for measuring components contained in an aerosol according to any one of claims 1 to 4.

(Appendix 6)
the reaction solution further comprises an electron mediator;
In the step (d), the changed third substance is returned to its original state by an electrochemical method in the presence of an electron mediator.
A method for measuring components contained in an aerosol according to any one of claims 1 to 5.

(Appendix 7)
The reaction solution contains the first substance in an excess amount relative to the target component.
A method for measuring components contained in an aerosol according to any one of claims 1 to 6.

(Appendix 8)
The reaction solution contains the electron mediator in an excess amount relative to the target component.
A method for measuring the components contained in the aerosol described in Appendix 6.

(Appendix 9)
The step (b) is carried out until the amount of the second substance produced becomes greater than the amount of the target component.
A method for measuring components contained in an aerosol according to any one of claims 1 to 8.

(Appendix 10)
In the step (d), a threshold value of the current value is set, and the quality of the environmental atmosphere is judged based on the predetermined threshold value.
A method for measuring components contained in an aerosol according to any one of claims 1 to 9.

(Appendix 11)
An apparatus for measuring a target component contained in an aerosol in the atmosphere, comprising:
A reaction unit configured to accommodate a reaction liquid containing a first substance that reacts with the target component and to incorporate the aerosol into the reaction liquid;
A measurement unit that measures the reaction solution by an electrochemical method;
A determination unit that determines the presence or absence of the target component in the aerosol based on a result from the measurement unit,
In the reaction section, when the aerosol is taken into the reaction liquid, a second substance is generated by a reaction between the target component and the first substance, and the second substance is oxidized or reduced in the presence of a third substance;
The measurement unit measures a current value generated by returning the third substance, which has changed by contributing to the oxidation or reduction of the second substance in the reaction solution, to its original state by an electrochemical method.
A device for measuring the components contained in aerosols.

(Appendix 12)
the target component is amylase derived from saliva contained in the aerosol,
the first substance is at least one of amylose and amylopectin;
In the reaction section, glucose is generated as the second substance by a reaction between the first substance and amylase, and the glucose is oxidized in the presence of an oxidizing agent as the third substance;
The measurement unit measures a current value generated by returning the oxidant, which has been changed by contributing to the oxidation of the glucose, to its original state by an electrochemical method;
The determination unit evaluates the presence or absence of the amylase in the aerosol based on the current value.
An apparatus for measuring components contained in an aerosol according to claim 11.

(Appendix 13)
the target component is a component having neuraminidase in its chemical structure,
the first substance is sialyllactose and lactase;
In the reaction section, lactose is produced by a reaction between the neuraminidase and the sialyllactose, and glucose is produced as the second substance by a reaction between the lactose and the lactase, and the glucose is oxidized in the presence of an oxidizing agent as the third substance;
The measurement unit measures a current value generated by returning the oxidant, which has been changed by contributing to the oxidation of the glucose, to its original state by an electrochemical method;
The determination unit evaluates the presence or absence of the amylase in the aerosol based on the current value.
An apparatus for measuring components contained in an aerosol according to claim 11.

(Appendix 14)
The component having neuraminidase is at least one of influenza virus and Streptococcus pneumoniae.
An apparatus for measuring components contained in an aerosol according to claim 13.

(Appendix 15)
The reaction section is configured to expose the reaction liquid to an environmental atmosphere.
An apparatus for measuring components contained in an aerosol according to any one of claims 11 to 14.

(Appendix 16)
The reaction solution further comprises an electron mediator;
The measurement unit measures a current value generated by returning the changed third substance to its original state by an electrochemical method in the presence of the electron mediator.
An apparatus for measuring components contained in an aerosol according to any one of claims 11 to 15.

(Appendix 17)
The reaction section reacts the target component with the first substance until an amount of the second substance produced is greater than an amount of the target component.
An apparatus for measuring components contained in an aerosol according to any one of claims 11 to 16.

(Appendix 18)
A reaction solution for measuring amylase contained in an aerosol, comprising:
At least one of amylose and amylopectin, at least one of glucose oxidase and glucose dehydrogenase, and an electron mediator.
Reaction solution for amylase measurement.

(Appendix 19)
A mixing kit for preparing a reaction solution for measuring amylase contained in an aerosol, comprising:
At least one of amylose and amylopectin;
at least one of glucose oxidase and glucose dehydrogenase;
and an electron mediator, which are contained separately and are configured to be prepared as the reaction liquid by mixing.
A reaction mixture kit for amylase measurement.

(Appendix 20)
A reaction solution for measuring pathogens contained in aerosol,
the pathogen is at least one of an influenza virus and a Streptococcus pneumoniae having a neuraminidase in its chemical structure;
The present invention relates to a method for producing a lactase comprising the steps of: (a) extracting lactose from a lactose extract; (b) extracting lactose from a lactose extract; and (c) extracting lactose from a lactose extract.
Reaction solution for pathogen measurement.

(Appendix 21)
A mixing kit for preparing a reaction solution for measuring pathogens contained in aerosol, comprising:
the pathogen is at least one of an influenza virus and a Streptococcus pneumoniae having a neuraminidase in its chemical structure;
The reaction solution is prepared by mixing sialyllactose, lactase, at least one of glucose oxidase and glucose dehydrogenase, and an electron mediator, which are contained separately.
A mixed reaction solution kit for pathogen measurement.

REFERENCE SIGNS LIST 1 Measuring device 10 Reaction section 20 Measurement section 21 Working electrode 22 Counter electrode 23 Reference electrode 24 Potential sweep section 25 Current measurement section 30 Determination section 31 Determination processing section 32 Memory section 40 Control section S Reaction liquid

Claims (18)

A method for measuring a target component contained in an aerosol in an atmosphere, comprising:
(a) preparing a reaction solution containing a first substance that reacts with the target component;
(b) taking in the aerosol in the reaction liquid, reacting the target component with the first substance, and generating a second substance;
(c) oxidizing or reducing the second substance in the presence of a third substance;
(d) evaluating the presence or absence of the target component in the aerosol based on a current value generated by returning the third substance, which has been changed by contributing to the oxidation or reduction of the second substance, to its original state by an electrochemical method;
A method for measuring the components contained in aerosols. the target component is amylase derived from saliva contained in the aerosol,
the first substance is at least one of amylose and amylopectin;
In the step (b), an aerosol containing the amylase is introduced into the reaction liquid, and the first substance is hydrolyzed by the amylase to generate glucose as the second substance;
In the step (c), the glucose is oxidized in the presence of an oxidizing agent as the third substance;
In the step (d), the presence or absence of the amylase in the aerosol is determined based on a current value generated by returning the oxidizing agent, which has been changed by contributing to the oxidation of the glucose, to its original state by an electrochemical method.
A method for measuring components contained in the aerosol according to claim 1. the target component is a component having neuraminidase in its chemical structure,
the first substance is sialyllactose and lactase;
In the step (b), an aerosol containing a component having the neuraminidase is introduced into the reaction liquid, the neuraminidase is hydrolyzed to lactose by the sialyllactose, and glucose is produced as the second substance from the lactose by the lactase;
In the step (c), the glucose is oxidized in the presence of an oxidizing agent as the third substance;
and (d) determining the presence or absence of the neuraminidase in the aerosol based on a current value generated by returning the oxidizing agent, which has been changed by contributing to the oxidation of the glucose, to its original state by an electrochemical method.
A method for measuring components contained in the aerosol according to claim 1. In the step (b), the reaction liquid is placed so as to be exposed to the ambient atmosphere.
A method for measuring components contained in the aerosol according to claim 1. the reaction solution further comprises an electron mediator;
In the step (d), the changed third substance is returned to its original state by an electrochemical method in the presence of an electron mediator.
A method for measuring components contained in the aerosol according to claim 1. The reaction solution contains the first substance in an excess amount relative to the target component.
A method for measuring components contained in the aerosol according to claim 1. The reaction solution contains the electron mediator in an excess amount relative to the target component.
A method for measuring components contained in an aerosol according to claim 5. The step (b) is performed until the amount of the second substance produced becomes greater than the amount of the target component.
A method for measuring components contained in the aerosol according to claim 1. An apparatus for measuring a target component contained in an aerosol in the atmosphere, comprising:
A reaction unit configured to accommodate a reaction liquid containing a first substance that reacts with the target component and to incorporate the aerosol into the reaction liquid;
A measurement unit that measures the reaction solution by an electrochemical method;
A determination unit that determines the presence or absence of the target component in the aerosol based on a result from the measurement unit,
In the reaction section, when the aerosol is taken into the reaction liquid, a second substance is generated by a reaction between the target component and the first substance, and the second substance is oxidized or reduced in the presence of a third substance;
The measurement unit measures a current value generated by returning the third substance, which has changed by contributing to the oxidation or reduction of the second substance in the reaction solution, to its original state by an electrochemical method.
A device for measuring the components contained in aerosols. the target component is amylase derived from saliva contained in the aerosol,
the first substance is at least one of amylose and amylopectin;
In the reaction section, the first substance is hydrolyzed by the amylase to generate glucose as the second substance, and the glucose is oxidized in the presence of an oxidizing agent as the third substance;
The measurement unit measures a current value generated by returning the oxidant, which has been changed by contributing to the oxidation of the glucose, to its original state by an electrochemical method;
The determination unit evaluates the presence or absence of the amylase in the aerosol based on the current value.
An apparatus for measuring components contained in an aerosol according to claim 9. the target component is a component having neuraminidase in its chemical structure,
the first substance is sialyllactose and lactase;
In the reaction section, lactose is produced by a reaction between the neuraminidase and the sialyllactose, and glucose is produced as the second substance by a reaction between the lactose and the lactase, and the glucose is oxidized in the presence of an oxidizing agent as the third substance;
The measurement unit measures a current value generated by returning the oxidant, which has been changed by contributing to the oxidation of the glucose, to its original state by an electrochemical method;
The determination unit evaluates the presence or absence of the neuraminidase in the aerosol based on the current value.
An apparatus for measuring components contained in an aerosol according to claim 9. The reaction section is configured to expose the reaction liquid to an environmental atmosphere.
An apparatus for measuring components contained in an aerosol according to claim 9. The reaction solution further comprises an electron mediator;
The measurement unit measures a current value generated by returning the changed third substance to its original state by an electrochemical method in the presence of the electron mediator.
An apparatus for measuring components contained in an aerosol according to claim 9. The reaction unit reacts the target component with the first substance until the amount of the second substance produced is greater than the amount of the target component.
An apparatus for measuring components contained in an aerosol according to claim 9. A reaction solution for measuring amylase contained in an aerosol, comprising:
At least one of amylose and amylopectin, at least one of glucose oxidase and glucose dehydrogenase, and an electron mediator.
Reaction solution for amylase measurement. A mixing kit for preparing a reaction solution for measuring amylase contained in an aerosol, comprising:
At least one of amylose and amylopectin;
at least one of glucose oxidase and glucose dehydrogenase;
and an electron mediator, which are contained separately and are configured to be prepared as the reaction liquid by mixing.
A reaction mixture kit for amylase measurement. A reaction solution for measuring pathogens contained in aerosol,
the pathogen is at least one of an influenza virus and a Streptococcus pneumoniae having a neuraminidase in its chemical structure;
The present invention relates to a method for producing a lactase comprising the steps of: (a) extracting lactose from a lactose extract; (b) extracting lactose from a lactose extract; and (c) extracting lactose from a lactose extract.
Reaction solution for pathogen measurement. A mixing kit for preparing a reaction solution for measuring pathogens contained in aerosol, comprising:
the pathogen is at least one of an influenza virus and a Streptococcus pneumoniae having a neuraminidase in its chemical structure;
The reaction solution is prepared by mixing sialyllactose, lactase, at least one of glucose oxidase and glucose dehydrogenase, and an electron mediator, which are contained separately.
A mixed reaction solution kit for pathogen measurement.

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