JPH0445723A - Preparation of culture medium for culturing mushroom - Google Patents
Preparation of culture medium for culturing mushroomInfo
- Publication number
- JPH0445723A JPH0445723A JP2150905A JP15090590A JPH0445723A JP H0445723 A JPH0445723 A JP H0445723A JP 2150905 A JP2150905 A JP 2150905A JP 15090590 A JP15090590 A JP 15090590A JP H0445723 A JPH0445723 A JP H0445723A
- Authority
- JP
- Japan
- Prior art keywords
- culture
- bag
- culture medium
- gas
- sealing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 29
- 239000001963 growth medium Substances 0.000 title claims description 49
- 238000012258 culturing Methods 0.000 title claims description 4
- 238000007789 sealing Methods 0.000 claims abstract description 20
- 239000000463 material Substances 0.000 claims abstract description 9
- 238000004519 manufacturing process Methods 0.000 claims description 27
- 239000002054 inoculum Substances 0.000 claims description 17
- 238000011081 inoculation Methods 0.000 abstract description 22
- 239000007789 gas Substances 0.000 abstract description 10
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 abstract 1
- 238000005273 aeration Methods 0.000 abstract 1
- 229910001882 dioxygen Inorganic materials 0.000 abstract 1
- 238000004826 seaming Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 description 14
- 241000894006 Bacteria Species 0.000 description 8
- 238000009958 sewing Methods 0.000 description 7
- 238000003860 storage Methods 0.000 description 5
- 238000011109 contamination Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000009423 ventilation Methods 0.000 description 3
- 206010021143 Hypoxia Diseases 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000009776 industrial production Methods 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- -1 (22)...Sealing part Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000007726 management method Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
Landscapes
- Mushroom Cultivation (AREA)
Abstract
Description
【発明の詳細な説明】
（産業上の利用分野）
本発明は、きのこ等の人工栽培用培基の製法に係り、特
に、きのこ種菌の接種を行った後、培養工程に至るまで
の生産工程において、その間無菌室でなくても培養、保
管できる様にすることにより、生産拠点の分離による生
産の合理性が図れると共に、培養日程がコントロールで
きるきのこ栽培用培養基の製法に関する。Detailed Description of the Invention (Industrial Field of Application) The present invention relates to a method for producing a culture medium for artificial cultivation of mushrooms, etc., and particularly relates to a production process from inoculation of a mushroom seed to the cultivation process. The present invention relates to a method for producing a culture medium for mushroom cultivation, which enables cultivation and storage without the need for a sterile room during the period, thereby increasing rationality in production by separating production bases, and in which the cultivation schedule can be controlled.
（従来の技術）
一般的にきのこ等の人工栽培用培基の工業的生産では、
まず容器内に培基原料を詰め、きのこ種菌を接種して栽
培するという方法がとられる場合が多い、一般の工業生
産においては、ローコスト化を図るため第３図に示す様
にフィルム状の透明な培養袋（１）を用い、その上部開
口をリング状の肩口（２）内に遣して外側に折り返し、
内部にフィルター（３）を有するキャップ（４）を肩口
（２）にはめ合わせ、培養袋内部に雑菌が侵入しない様
にしている。しかし実際には肩口、フィルターは何回も
使用してランニングコストの低廉化を図っているため、
特にフィルターは洗浄、乾燥を繰り返しながら何回も使
用するに従って、その寸法が収縮し、その隙間から雑菌
が侵入するようになってくる。このため、リサイクルの
使用固数にも限度があり、又、フィルター自体も雑菌侵
入を完全に防いでいる訳でないため、外気中に長時間に
わたって接触させる事は汚染率を高める要因となる。従
って一般的には種菌を接種する工程（以下植菌工程と呼
ぶ）は室内を無菌状態に保ち雑菌の汚染を防いでいる。(Conventional technology) In general, in the industrial production of culture medium for artificial cultivation of mushrooms, etc.
In general industrial production, the method of first filling a container with culture material, inoculating it with mushroom starter, and cultivating it is to reduce costs. Using a culture bag (1), put the top opening into the ring-shaped shoulder opening (2) and fold it back outward.
A cap (4) having a filter (3) inside is fitted onto the shoulder opening (2) to prevent bacteria from entering the culture bag. However, in reality, shoulder openings and filters are used many times to reduce running costs.
In particular, as filters are repeatedly washed and dried and used many times, their dimensions shrink, allowing bacteria to enter through the gaps. For this reason, there is a limit to the number of filters that can be recycled, and since the filter itself does not completely prevent the intrusion of bacteria, prolonged contact with the outside air will increase the contamination rate. Therefore, in general, the process of inoculating seed bacteria (hereinafter referred to as the inoculation process) keeps the room sterile and prevents contamination with various germs.
したがって植菌後は該袋をただちに培養倉庫に移し、種
菌蔓延に要する期間だけ培養倉庫に収納しておくといっ
た方法がとられている。Therefore, after inoculation, the bags are immediately moved to a culture warehouse and stored there for the period required for the inoculum to spread.
（発明が解決しようとする問題点）
しかし、近年市街地においては、地価コストの上昇、ス
ペース不足等の制約によって大規模な生産工場の建設が
困難な状況となってきている。−方、きのこ等の種菌蔓
延日数は、１０〜６０日程度必要とするため、その期間
のきのこの収納・保管スペースを考えると大規模な建物
を準備する必要がある。(Problems to be Solved by the Invention) However, in recent years, it has become difficult to construct large-scale production factories in urban areas due to constraints such as rising land prices and lack of space. On the other hand, since it takes about 10 to 60 days for the inoculum to spread, such as mushrooms, etc., it is necessary to prepare a large-scale building considering the storage space for mushrooms during that period.
このため、輸送コストが加わっても市街地がら離れた地
価の安い地域に培養倉庫を建て、植菌後の培養基を運ん
だ方が得策となってきた。For this reason, even with the added cost of transportation, it has become a better idea to build culture warehouses in areas where land prices are low, away from urban areas, and transport the culture medium after inoculation.
しかしながら、前述した従来技術による生産方法では、
植菌を行った後に該培養基を外部に持ち出し、外気に接
触させる事は、雑菌汚染の危険性が高まり、問題であっ
た。この場合、無菌状態で輸送する手段があれば良いが
一般的な輸送手段、たとえばトラック等の自動車類、貨
車、船舶等で植菌後の培養基を汚染せずに安全に輸送す
るための手段は見い出されていない、又、培養基を１つ
１つ別の袋に入れて密封する方法や、スポンジ部分を完
全に塞ぐ方法を採っても可能ではあるが、大量に処理す
る際には手間がかかるという問題が新たに生じるため、
有効な手段ではない。However, in the production method using the conventional technology described above,
Taking the culture medium outside and exposing it to the outside air after inoculation increases the risk of bacterial contamination, which is a problem. In this case, it is only necessary to have a means of transporting the culture medium in a sterile state, but there are ways to safely transport the culture medium after inoculation without contaminating it using general means of transport, such as automobiles such as trucks, freight cars, ships, etc. This has not been found yet.Also, it is possible to put each culture medium in a separate bag and seal it, or to completely block the sponge part, but this is time-consuming when processing large quantities. Because a new problem arises,
It's not an effective method.
即ち外気に存在する雑菌から培養基を完全に守り、かつ
処理の楽な培養基形状とその生産方法の確立が強く望ま
れている現状である。In other words, there is a strong desire to establish a culture medium shape and production method that completely protects the culture medium from germs present in the outside air and is easy to process.
本発明は、前述した従来技術の問題点に鑑みなされたも
ので、植菌後から培養工程に至るまでの生産工程に於て
、その間無菌室でなくても栽培できる様にすることによ
り、生産拠点の分離による経済性が図れると共に、培養
日程がコントロールできる栽培用培養基の製法を提供す
ることを目的とする。The present invention was made in view of the problems of the prior art described above, and it improves production by making it possible to perform cultivation without the need for a sterile room during the production process from inoculation to the cultivation process. The purpose of the present invention is to provide a method for producing a culture medium for cultivation that is economical by separating bases and allows control of the culture schedule.
（問題点を解決するための手段）
前記問題点を解決すべく本発明者等は、鋭意検討を重ね
た結果、有効な手段を見い出す事に成功した。その製法
は、袋形状を有する容器内に気体を流入した後、開口部
をシール密閉し、内部に蓄えた気体を菌糸生長に利用し
ながら培養することにより達成される。又、その際に、
前記袋内空間部に、菌糸の成育に必要な気体を封入する
ことにより培養日程を自由にコントロールすることが出
来る。(Means for Solving the Problems) In order to solve the above-mentioned problems, the inventors of the present invention have made extensive studies and have succeeded in finding an effective means. The manufacturing method is achieved by introducing gas into a bag-shaped container, sealing the opening, and culturing while using the gas stored inside for mycelial growth. Also, at that time,
By filling the inner space of the bag with gas necessary for the growth of hyphae, the culture schedule can be freely controlled.
又、他の製法は、まずフィルム状材からなる袋内にきの
こ栽培用培養基において、きのこ種菌を接種する工程と
、上記袋を閉じてシールする工程と、シールされた位置
と培養基との間に糸によって縫合する工程とから達成さ
れ、シールして一旦密封した後に長時間放置または保管
した後で縫合を行う事ができ、更にはシールして密封し
た状態で遠隔地へ輸送した後に縫合を行う事もできる。In addition, another manufacturing method involves first inoculating mushroom inoculum in a culture medium for mushroom cultivation in a bag made of film-like material, then closing and sealing the bag, and adding a layer between the sealed position and the culture medium. This can be achieved through the process of suturing with thread, and suturing can be performed after being sealed and left for a long time or stored, or suturing can be performed after being sealed and transported to a remote location in a sealed state. I can do things.
即ち袋上端の開口部を一時的にシールを行い、密封状態
にする事により、保存、輸送等多方面にわたって取扱い
が自在に行える様になるものである。That is, by temporarily sealing the opening at the upper end of the bag to make it airtight, the bag can be handled freely in various ways such as storage and transportation.
（作用）
本発明によれば、種菌を接種した後のきのこ栽培用培養
基は、袋内に封入されているため、シール後は無菌状態
の空間を必要と七ず自由に一般大諷中に触れさせる事が
可能となる。このため、従来−船釣に行れているような
フィルター付キャンプを用いた培養基であれば周囲温度
変化による内外圧力差が生じた場合、フィルター周囲部
隊間（第１１ｆｆｌ中矢印Ａ）より外気が侵入し、雑菌
汚染を招く結果となるが、本発明の方法によればシール
により外気と完全に遮蔽されているためこの心配は全く
ない。更には種菌接種後、一定期間保存可能であるため
、培養基袋内部スペースや種菌残量に気を配る事なく、
生産維持可能となり、生産管理が円滑となり得る。(Function) According to the present invention, since the culture medium for mushroom cultivation after inoculation with the inoculum is sealed in the bag, it can be freely accessed without requiring a sterile space after sealing. It becomes possible to do so. For this reason, if a culture medium is used in a camp with a filter, as is the case with conventional boat fishing, if a difference in pressure between the inside and outside occurs due to a change in ambient temperature, the outside air will flow between the sections surrounding the filter (arrow A in 11th ffl). However, according to the method of the present invention, there is no need to worry about this because the seal is completely shielded from the outside air. Furthermore, since it can be stored for a certain period of time after inoculation, there is no need to worry about the space inside the culture medium bag or the remaining amount of the inoculum.
Production can be maintained and production management can become smooth.
（実施例）
本発明の一実施例を図面を参照しながら詳細に説明する
。(Example) An example of the present invention will be described in detail with reference to the drawings.
第１図は本発明に係るきのこ培養基の製造プロセスを示
すもので、第１図（ａ）〜（ｃ）工程までの一連の工程
は無菌室で行なわれるものであり、（ａ）工程は、袋内
にきのこ培地原料を充填し、種菌を接種する工程、（ｂ
）工程は、この袋を完全密封する工程、（ｃ）は、本発
明に係る製法によって作られた培養基の形態を示すもの
で、このシール後は、外気にさらしても雑菌に汚染され
ることはない。FIG. 1 shows a process for manufacturing a mushroom culture medium according to the present invention, and a series of steps from steps (a) to (c) in FIG. 1 are performed in a sterile room, and step (a) is Filling the bag with mushroom culture material and inoculating the seed culture, (b
Step ) is the step of completely sealing this bag, and step (c) shows the form of the culture medium produced by the manufacturing method according to the present invention, and after this sealing, it will not be contaminated with bacteria even if exposed to the outside air. There isn't.
（ｄ）工程は、賃上部のシールされた位置と培養基との
間を糸によって縫合する工程で、植菌後５〜１５日以内
にこの縫製をかけ袋内に通気孔を設けることによって菌
糸成長時の酸欠を防いでいる。The (d) step is the step of sewing the sealed position on the upper part and the culture medium with thread. Within 5 to 15 days after inoculation, this sewing is done and ventilation holes are provided in the bag to allow mycelia to grow. Prevents oxygen deficiency.
尚、これらの図には示していないが（ｂ）工程でシール
する際に、袋内に無菌空気又は、菌糸の生長に好ましい
気体を封入してシールすることにより、シール後の菌糸
の生長期間をコントロールすることが出来る。Although not shown in these figures, when sealing in step (b), by sealing the bag with sterile air or a gas favorable for the growth of hyphae, the growth period of hyphae after sealing can be reduced. can be controlled.
第２図は本発明によるきのこ栽培用培養基における種菌
接種工程および培養工程を概念的に示した図である。１
ｌｉｌ圧かつ無菌状態に保たれた種菌接種室（５）内部
に搬送コンベア（６）を通じて開口部（７）より搬入さ
れた培養基は、接種機（８）によって種菌が植えつけら
れる。その後シール機（９）により開口部を閉じるとと
もに完全密封の状態とし、その状態で搬出コンベア（１
０）によって開口部（１１）より室外に搬出される。な
お、第１図に示すように培養基はシール機（９）により
、内部に空気を残存させた状態で賃上部開口部を完全に
密閉させるため、容器内外の通気は、袋が破損しない限
り全くない、その後は、特別の無菌室を何ら設けること
なく一般に用いられている自動車や貨車、船舶等の輸送
手段（１２）により、種菌接種に要した建物から離れた
場所まで培養基を輸送し縫製機（１４）によって縫合し
た後、培養倉庫（１３）に収納する。FIG. 2 is a diagram conceptually showing the inoculum inoculation step and culture step in the culture medium for mushroom cultivation according to the present invention. 1
The culture medium is carried through the opening (7) through the transport conveyor (6) into the inoculum inoculation chamber (5), which is kept under sterile conditions under lil pressure, and is inoculated with the inoculum by the inoculating machine (8). After that, the opening is closed by the sealing machine (9) and completely sealed, and in this state, the unloading conveyor (1
0) is carried outside the room through the opening (11). As shown in Figure 1, the culture medium is sealed using a sealing machine (9) to completely seal the top opening with air remaining inside, so there is no ventilation inside or outside the container unless the bag is damaged. After that, the culture medium is transported to a location far from the building required for inoculation using commonly used means of transportation (12), such as automobiles, freight cars, and ships, without setting up a special sterile room, and the sewing machine After suturing by (14), it is stored in the culture warehouse (13).
この時培養基を外気に接触させる事になるが、袋が破損
しない限り内部への雑菌侵入はない。At this time, the culture medium will come into contact with the outside air, but as long as the bag is not damaged, no bacteria will enter the bag.
なお、第３図に示す従来のフィルターを備えたキャップ
を°使用した培養基においては、外部からの雑菌侵入を
完全に止める事はできない、たとえばフィルター材とキ
ャップの嵌合が少しでもゆるめだとフィルターの除菌効
果が有効的に発揮されずに直接外気が培養基に触れる事
となる。この現象は輸送申培養基に温度変化を与えると
顕著に発生する場合が多い、つまり、培養基袋内部の空
気圧が温度変化により、変動するため袋内圧が外部圧力
より低くなった場合、外気が培養基内部に吸引される。It should be noted that in a culture medium using a cap equipped with a conventional filter as shown in Figure 3, it is not possible to completely prevent the intrusion of bacteria from the outside.For example, if the fit between the filter material and the cap is even slightly loose, the filter The sterilizing effect of the culture medium is not effectively exerted and the outside air comes into direct contact with the culture medium. This phenomenon often occurs noticeably when a temperature change is applied to the culture medium during transport.In other words, the air pressure inside the culture medium bag fluctuates due to temperature changes, so if the internal pressure of the bag becomes lower than the external pressure, the outside air will move inside the culture medium. is attracted to.
この時外気は除菌効果のないフィルター周囲部より侵入
する事となる。このため、培養基の輸送を考えた場合、
温度変化を与えない手段が必要となる。これに対し、本
発明の手段によれば、培養基内部種菌が死滅しないＯ℃
〜３０℃の温度範囲内であれば自由に移す事が可能であ
るため、輸送手段に特殊な！Ｊ置を必要としない。At this time, outside air enters from the area around the filter, which has no sterilization effect. Therefore, when considering the transportation of culture medium,
A means that does not cause temperature changes is required. On the other hand, according to the means of the present invention, the inoculum inside the culture medium is not killed.
It can be moved freely within the temperature range of ~30℃, making it a special means of transportation! J position is not required.
又、本発明を実施するにあたり、種菌接種後の輸送距離
や所要時間に関し、制限を全く受けない。Further, in carrying out the present invention, there are no restrictions at all regarding the transportation distance or required time after inoculation of the inoculum.
ただし、接種後の菌は、培養袋内に存在する酸素を使っ
て生長するため、植菌後５〜１５日以内には縫製をかけ
、通気手段を確保させて酸欠を防いでやる必要がある。However, since the bacteria after inoculation grow using the oxygen present in the culture bag, it is necessary to sew the bag within 5 to 15 days after inoculation to ensure ventilation and prevent oxygen deficiency. be.
又、きのこに種菌接種ｖｋ袋をソールする際に、その空
間部に菌糸の成育に好ましい気体、例えば酸素と適正な
濃度となるよう封入すれば、培養日程、成育環境をある
程度自由にコントロールすることが出来る。In addition, when placing the inoculum inoculation bag on mushrooms, if the space is filled with a gas suitable for the growth of mycelia, such as oxygen, at an appropriate concentration, the culture schedule and growth environment can be controlled to a certain degree. I can do it.
又、縫合工程において、袋の開口部が開口のままである
と、これを閉して縫合する際、衝合した袋の表裏が滑り
易いため、縫合ミスを起し易いが、本発明に於てはシー
ルにより袋の上部が固定されているため、縫合作業が容
易に出来る利点を有する。In addition, in the sewing process, if the opening of the bag remains open, when it is closed and sutured, the front and back sides of the bag are likely to slip, which can easily cause sewing errors. Since the upper part of the bag is fixed with a seal, it has the advantage of making suturing work easier.
（発明の効果）
本発明によれば、袋内の培養基に種菌を接種後その上部
に一定の空間を設けて開口部を全面シールしているため
、シール後は種菌接種工程と培養工程とを別々の場所に
て行う事ができる。従って、これらの生産拠点を経済性
を考慮し建設することが出来、従来の生産工場建設にか
かわるコスト・スペースの立地条件が大幅に拡大する他
、既に生産を行っている一般の生産工場においても、培
養倉庫の収納量が満杯状態時でも仮保存が可能であるた
め、種菌接種工程を通常遺り維持する事ができる。即ち
生産計画の円滑単純化に大きく貢献する事ができる。(Effects of the Invention) According to the present invention, after inoculating the inoculum into the culture medium in the bag, a certain space is provided above it and the opening is completely sealed, so that after sealing, the inoculum inoculation process and the culturing process are performed. This can be done at different locations. Therefore, these production bases can be constructed with economic efficiency in mind, and in addition to significantly increasing the cost and space requirements for constructing conventional production factories, it is also possible to construct these production bases with consideration to economic efficiency. Since temporary storage is possible even when the storage capacity of the culture warehouse is full, the seed inoculation process can be maintained normally. In other words, it can greatly contribute to smooth and simple production planning.
第１図は、本発明に係るきのこ栽培用培養基の製造プロ
セスとその培養基の形態を示す説明図、第２図は、本発
明に係るきのこ培養基の生産工程概念図、第３図は、従
来一般に用いられているきのこ人工栽培に用いる培養基
の形態を示す。
（１） −・・培養袋、
（３）・・・フィルター
（５） −・・種菌接種室、
（７） ・・・開口部（入口側）
（８） −・・種菌接種機、
（１１）−・・開口部（出口側）
（１２）−・・培養基輸送手段、
（１４）・・・縫製機、
（２１）−・・袋内部気体、
（２３）・・・縫製部。
（２） −・・肩口リング、
（４） −・・キャップ、
（６）（１０） −・・搬送コンベア、（９） −・
・シール手段、
（１３）・・・培養倉庫、
（２０）・・・培養基材料、
（２２）・・・シール部、
種菌接種室
第
図
第
図
ムFIG. 1 is an explanatory diagram showing the manufacturing process of a culture medium for mushroom cultivation according to the present invention and the form of the culture medium. FIG. 2 is a conceptual diagram of the production process of a mushroom culture medium according to the present invention. FIG. The form of the culture medium used for artificial cultivation of mushrooms is shown. (1) - Culture bag, (3) Filter (5) - Inoculum inoculation chamber, (7) Opening (inlet side) (8) - Inoculum inoculator, (11 )--Opening (exit side) (12)--Culture medium transport means, (14)--Sewing machine, (21)--Bag internal gas, (23)--Sewing section. (2) --- Shoulder ring, (4) --- Cap, (6) (10) --- Conveyor, (9) ---
・Sealing means, (13)...Culture warehouse, (20)...Culture medium material, (22)...Sealing part, Inoculum inoculation chamber
Claims (5)
部をシール密閉し、内部に蓄えた気体を菌糸生長に利用
しながら培養を行うことを特徴とするきのこ栽培用培養
基の製法。(1) A method for producing a culture medium for mushroom cultivation, which comprises introducing gas into a bag-shaped container, sealing the opening, and culturing while using the gas stored inside for mycelial growth.
てなる特許請求の範囲第１項記載のきのこ栽培用培養基
の製法。(2) The method for producing a culture medium for mushroom cultivation according to claim 1, wherein the gas in the bag encapsulates a gas necessary for the growth of hyphae.
れたきのこ栽培用培養基において、きのこ種菌を接種す
る工程と、上記袋を閉じてシールする工程と、シールさ
れた位置と培養基との間に糸によって縫合する工程とよ
りなることを特徴とするきのこ栽培用培養基の製法。(3) In a culture medium for mushroom cultivation in which a mushroom culture medium is filled in a bag made of a film-like material, a step of inoculating the mushroom inoculum, a step of closing and sealing the bag, and a gap between the sealed position and the culture medium. A method for producing a culture medium for mushroom cultivation, characterized by a step of suturing with thread.
しくは保管した後で縫合を行うことを特徴とする特許請
求の範囲第３項記載のきのこ栽培用培養基の製法。(4) The method for producing a culture medium for mushroom cultivation according to claim 3, wherein the bag opening is sealed and sealed, and then the bag is left to stand or stored for a long period of time, and then sutured.
し、縫合を行った後に培養を行うことを特徴とする特許
請求の範囲第３項または第４項記載のきのこ栽培用培養
基の製法。(5) The culture medium for mushroom cultivation according to claim 3 or 4, wherein the culture medium is cultured after sealing the opening of the bag, transporting it to a remote location, and performing sutures. manufacturing method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2150905A JPH0445723A (en) | 1990-06-09 | 1990-06-09 | Preparation of culture medium for culturing mushroom |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2150905A JPH0445723A (en) | 1990-06-09 | 1990-06-09 | Preparation of culture medium for culturing mushroom |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0445723A true JPH0445723A (en) | 1992-02-14 |
Family
ID=15506952
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2150905A Pending JPH0445723A (en) | 1990-06-09 | 1990-06-09 | Preparation of culture medium for culturing mushroom |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0445723A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009136183A (en) * | 2007-12-05 | 2009-06-25 | Ueda Sangyo Kk | Line device for inoculation feeding of retort culture medium in artificial mushroom cultivation |
| JP2014008048A (en) * | 2012-07-03 | 2014-01-20 | Ueda Sangyo Kk | Method for artificially cultivating flammulina velutipes mushrooms |
| US20150366141A1 (en) * | 2013-11-15 | 2015-12-24 | Donnie Lee Creekmore | Apparatus, system and method for producing fungi for use in a ecosystem |
-
1990
- 1990-06-09 JP JP2150905A patent/JPH0445723A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009136183A (en) * | 2007-12-05 | 2009-06-25 | Ueda Sangyo Kk | Line device for inoculation feeding of retort culture medium in artificial mushroom cultivation |
| JP2014008048A (en) * | 2012-07-03 | 2014-01-20 | Ueda Sangyo Kk | Method for artificially cultivating flammulina velutipes mushrooms |
| US20150366141A1 (en) * | 2013-11-15 | 2015-12-24 | Donnie Lee Creekmore | Apparatus, system and method for producing fungi for use in a ecosystem |
| US9386751B2 (en) * | 2013-11-15 | 2016-07-12 | Donnie Lee Creekmore | Apparatus, system and method for producing fungi for use in a ecosystem |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4063383A (en) | Production of mushroom spawn | |
| EP0645078A1 (en) | Improved bag for the cultivation of mushroom spawn | |
| US4977702A (en) | Process for growing Pleurotus | |
| JPH0445723A (en) | Preparation of culture medium for culturing mushroom | |
| JPH08172906A (en) | Method for culturing lentinus edodes and bag for culturing lentinus edodes | |
| JPH0494621A (en) | Method and tool for culturing mushroom | |
| JP2003153632A (en) | Method for cultivating mushroom | |
| JP2023030914A (en) | Fungal bed cultivation bag | |
| CN207040407U (en) | Edible mushroom glue bottle | |
| KR102477296B1 (en) | The Smart Farm Block Automation Mushroom Cultivation Method | |
| JP3205751B2 (en) | Mushroom cultivation container and cultivation method using the same | |
| JPH053724A (en) | Culture vessel for mushroom | |
| JPH0371085B2 (en) | ||
| JP3241990U (en) | Fungus bed cultivation bag | |
| JPH09201130A (en) | Cultivation of mushroom | |
| JPS6019553Y2 (en) | container for mushroom cultivation | |
| CN205470553U (en) | Domestic fungus is planted and is used seal closure | |
| JP2005102521A (en) | Artificial cultivation method for mushroom | |
| JP2000125660A (en) | Automatic inoculation of mushroom | |
| JPH0789796B2 (en) | Sealing method for mushroom bag | |
| JPH0449879Y2 (en) | ||
| JPH02273125A (en) | Production of artificial culture medium of mushroom | |
| JPH08298862A (en) | Mushroom cultivation bag for automation and cultivation of fungal bed | |
| JPH0690622A (en) | Production of mushroom bed by film bag method | |
| JPH04187028A (en) | Vessel for culturing mushroom |