JPH05322882A - Blood analyzer - Google Patents

Abstract

(57) [Summary] [Objective] To realize a blood analyzer not only capable of performing leukocyte classification, reticulocyte measurement, and blood cell counting by one unit, but also improving accuracy of analysis results. [Structure] Scattered light and fluorescence from the sample liquid 25 in the flow cell 11 are detected by detectors 6, 7, and 8.
The fluorescence intensity signal from the detector 6 is supplied to the white blood cell classification unit 9a, and white blood cell classification is performed. The fluorescence intensity signal from the detector 7 is supplied to the reticulocyte counting section 9b to count reticulocytes. The scattered light intensity signal from the detector 8 is supplied to the white blood cell counting unit 9c, the red blood cell counting unit 9d, and the platelet counting unit 9e to count white blood cells, red blood cells, and platelets.
The results of the classification unit 9a and the counting unit 9c are supplied to the white blood cell count correction unit 9f to correct the number of white blood cells, and a highly accurate white blood cell classification result is calculated. Counters 9b, 9d, 9e
The result of is supplied to the red blood cell count correction unit 9g and corrected,
A highly accurate reticulocyte count result is calculated.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a blood analyzer for analyzing blood cells, and more particularly to analysis and counting of each cell based on changes in scattered light intensity, fluorescence intensity or electric resistance value obtained from individual cells. The present invention relates to a blood analyzer.

[0002]

2. Description of the Related Art Analytical items in clinical tests in the field of hematology include leukocyte classification for obtaining the classification ratio of each subclass of white blood cells (lymphocyte, monocyte, neutrophil, eosinophil, basophil). Alternatively, there are items of reticulocyte measurement for obtaining the ratio of reticulocytes in red blood cells, and counting of the number of red blood cells, white blood cells and platelets, and measurement of hemoglobin concentration.

Among the above-mentioned analysis items, a white blood cell classification apparatus using a flow method is described in Japanese Patent Application Laid-Open No. 61-502277. In this publication, red blood cells are lysed, and white blood cells are classified by a combination of white blood cell DC volume, RF size, and light scattering. A reticulocyte measuring device is described in JP-A-62-34058. In this publication, the fluorescent dye auramine O is used as a staining reagent, and the reticulocyte in the reticulocytes is stained with this staining reagent. Then, fluorescence emitted from the stained reticulate body is measured, and reticulocyte measurement is performed.

Japanese Examined Patent Publication (Kokoku) No. 59-853 discloses an analyzer for performing the above-mentioned classification and measurement by one unit. In this publication, from the combination of green fluorescence and red fluorescence emitted from cells, blood cells that emit green fluorescence and red fluorescence are white blood cells, blood cells that do not emit green fluorescence are reticulocytes, and white blood cells and reticulocytes can be distinguished. Has been done.

An apparatus for performing blood cell counting is described in Japanese Patent Publication No. 54-2598. In this publication, changes in electrical resistance value that occur when red blood cells, white blood cells, platelets, etc. pass through the detection region are detected, and the detected resistance changes are subjected to pulse height analysis to count blood cells.

If there is no such classification or measuring device as described above, slide specimens are prepared by smearing blood for each specimen, stained with a special dye, and then individual cells are counted under a microscope. White blood cell classification or reticulocyte measurement must be performed. This requires a great deal of effort and time. Therefore, the classification or measuring device as described above is very important in blood analysis.

[0007]

By the way, it would be convenient to have a blood analyzer capable of performing leukocyte classification, reticulocyte measurement, and blood cell counting by one unit. Therefore, in the apparatus described in Japanese Patent Publication No. 59-853, the Japanese Patent Publication No. 54-25
It is conceivable to execute the operation of the device described in Japanese Patent Publication No. 98. However, since the device described in JP-B-59-853 and the device described in JP-B-54-2598 are completely different in blood diluting method and analysis method, they are described in JP-B-59-853. The device is not able to perform the blood cell count as described above. Therefore, conventionally, these three items of white blood cell classification, reticulocyte measurement, and blood cell count could not be performed by one analyzer. Therefore, each analysis item must be analyzed by a different device, and not only a technician is required for each device, but in order to analyze the above-mentioned three analysis items, reagents to be used and The blood volume had increased.

Further, in the conventional blood analyzer,
For example, when the number of white blood cells in blood is very large (leukemia patient), it is difficult to obtain an accurate classification ratio. That is, when the number of white blood cells is very large, the appearance frequency of abnormal spheres is high, which makes it difficult to perform accurate classification only by the result of normal white blood cell classification.

An object of the present invention is to realize a blood analyzer capable of performing leukocyte classification, reticulocyte measurement and blood cell counting by one unit. Another object of the present invention is not only that leukocyte classification, reticulocyte measurement, and blood cell counting can be performed by one unit, but also highly accurate even when the number of leukocytes in blood is very large. It is to realize a blood analyzer capable of calculating an analysis result such as a different classification ratio.

[0010]

In order to achieve the above object, the present invention is configured as follows. A reaction tank for mixing and reacting the blood sample solution and the diluted staining reagent,
A flow cell capable of passing cells in the sample liquid one by one through the detection region, a sample liquid distribution mechanism for supplying the sample liquid from the reaction tank to the flow cell, and a cell in the detection region of the flow cell. A light irradiation means for irradiating light, a detector for detecting scattered light or fluorescence generated from cells, and detecting information for white blood cell classification,
A detector that detects scattered light or fluorescence generated from cells and detects information for reticulocyte measurement, and a detector that detects scattered light or electrical resistance values generated from cells and detects information for blood cell counting Data processing means for calculating leukocyte classification data, reticulocyte measurement data and blood cell measurement data of a blood sample based on the detection signals output from the white blood cell classification detector, reticulocyte measurement detector and blood cell count detector And

Further, a reaction tank for mixing and reacting a blood sample solution and a diluted staining reagent, a flow cell capable of passing cells in the sample solution one by one through a detection region, and the sample solution From the reaction tank, a sample liquid distribution mechanism that supplies the flow cell to the flow cell, a resistance detector that detects the electrical resistance value of the sample liquid in the detection region of the flow cell, and based on a detection signal output from the resistance detector. Data processing means for calculating leukocyte classification data of a blood sample, reticulocyte measurement data and blood cell measurement data.

[0012] Preferably, the data processing means follows the output signals from the white blood cell sorting unit, reticulocyte counting unit, white blood cell counting unit, red blood cell counting unit, platelet counting unit, white blood cell sorting unit and white blood cell counting unit. A white blood cell count correction unit that corrects the white blood cell count and the white blood cell classification ratio, and a red blood cell count correction unit that corrects at least the red blood cell count according to output signals from the reticulocyte counter, the red blood cell counter, and the platelet counter.

Further, a reaction tank for mixing and reacting the blood sample solution and the diluted staining reagent, a flow cell capable of passing cells in the sample solution one by one through a detection region, and the sample solution From the reaction tank, a sample liquid distribution mechanism that supplies the flow cell to the flow cell, a light irradiation unit that irradiates the cell with light in the detection region of the flow cell, a detector that detects forward scattered light generated from the cell, and the cell A detector for detecting side scattered light generated from
From the detector that detects the green fluorescence generated from the cells, the detector that detects the red fluorescence generated from the cells, from the above forward scattered light detector, side scattered light detector, green fluorescence detector and red fluorescence detector Data processing means for calculating leukocyte classification data of the blood sample, reticulocyte measurement data, and blood cell measurement data based on the output detection signal. Preferably, the data processing means is a white blood cell classification unit, a reticulocyte counting unit, a white blood cell counting unit, a red blood cell counting unit, a platelet counting unit, and a white blood cell count according to an output signal from the white blood cell sorting unit and the white blood cell counting unit. The white blood cell classification unit includes a white blood cell count correction unit that corrects the white blood cell classification ratio, and a red blood cell count correction unit that corrects at least the red blood cell count according to the output signals from the reticulocyte counting unit, the red blood cell counting unit, and the platelet counting unit. , Output signals from the forward scattered light detector, the side scattered light detector, the green fluorescence detector and the red fluorescence detector are supplied, and the reticulocyte counting section is provided with the forward scattered light detector and the red fluorescence detector. Output signal is supplied,
An output signal from the forward scattered light detector is supplied to the white blood cell counting unit, the red blood cell counting unit, and the platelet counting unit.

[0014]

The data processing means includes the white blood cell classification data, reticulocyte measurement data and blood cell count data of the blood sample based on the output signals from the white blood cell classification detector, the reticulocyte measurement detector and the blood cell count detector. To calculate. As a result, a blood analyzer that can perform leukocyte classification, reticulocyte measurement, and blood cell counting with one unit is realized. Also,
Since the data processing means includes the white blood cell count correction unit and the red blood cell count correction unit, not only can leukocyte classification, reticulocyte measurement, and blood cell counting be performed by one unit, but, for example, the number of white blood cells in blood A blood analyzer capable of calculating an analysis result such as a highly accurate classification ratio even when the number of cases is very large is realized.

Further, the data processing means, based on the output signals from the forward scattered light detector, the side scattered light detector, the green fluorescence detector and the red fluorescence detector, the white blood cell classification data of the blood sample and the reticulocyte measurement. Data and blood count data are calculated. As a result, a blood analyzer that can perform leukocyte classification, reticulocyte measurement, and blood cell counting with one unit is realized. Further, since the data processing means is provided with the white blood cell count correction unit and the red blood cell count correction unit, not only can leukocyte classification, reticulocyte measurement and blood cell counting be performed by one unit, but also a highly accurate classification ratio. A blood analyzer capable of calculating an analysis result such as is realized.

[0016]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS FIG. 1 is a schematic block diagram of a blood analyzer according to an embodiment of the present invention, which is a flow-type blood analyzer. In FIG. 1, the blood in the blood collection tube is sucked by the sample suction mechanism 1 and is quantified in an amount necessary for white blood cell classification, reticulocyte measurement, and blood cell count, respectively. Each quantified blood is diluted and stained with each of the diluted staining reagents in the diluted staining mechanism 2 for classification of white blood cells, the diluted staining mechanism 3 for measuring reticulocytes, and the diluted staining mechanism 4 for counting blood cells. Each diluted and stained blood
The sample liquid is supplied to the sample liquid distribution mechanism 5 as a sample liquid.
The sample liquid distribution mechanism 5 has a structure as shown in FIG. That is, in FIG. 2, reference numeral 26 is a reaction tank, to which blood and a reagent such as a fluorescent dye are added, diluted, and stained. Then, the diluted and stained blood is stored as the sample liquid 25. In this case, the diluted staining reagent is separately used for leukocyte classification, reticulocyte measurement, and blood cell counting. In this way, after the pretreatment corresponding to each item is performed, the sample liquid 25 is supplied to the flow cell 11 by the sample distribution mechanism 5.

Now, in FIG. 1, the light from the light source 12 is applied to the sample liquid 25 in the flow cell 11.
Then, scattered light or fluorescence is generated from the cells in the sample liquid 25 in the flow cell 11. The light generated from the cells is detected by the white blood cell classification detector 6, the reticulocyte measurement detector 7, and the blood cell count detector 8. A signal indicating the detected fluorescence intensity is output from the detector 6, and this output signal is supplied to the white blood cell classification unit 9 a of the data processing unit 9. Then, the white blood cell classification unit 9a performs white blood cell classification. Also, from the detector 7,
A signal indicating the detected fluorescence intensity is output, and this output signal is supplied to the reticulocyte counting section 9b of the data processing section 9. Then, the reticulocyte counting section 9b counts reticulocytes. Further, the detector 8 outputs a signal indicating the detected scattered light intensity, and the output signal is supplied to the white blood cell counting unit 9c, the red blood cell counting unit 9d, and the platelet counting unit 9e of the data processing unit 9. .. Then, the white blood cells, red blood cells, and platelets are counted by the counting units 9c, 9d, and 9e, respectively.

The result of classification by the white blood cell classifying unit 9a and the result of counting by the white blood cell counting unit 9c are supplied to the white blood cell count correcting unit 9f. In the correction unit 9f, the number of white blood cells is corrected from the white blood cell classification result and the white blood cell count result, and an accurate white blood cell classification result is calculated from the corrected number. Then, a signal D1 indicating the corrected white blood cell count and the white blood cell classification result is output from the correction unit 9f and displayed by an appropriate display means (not shown). Further, the reticulocyte counting section 9b, the red blood cell counting section 9d, the platelet counting section 9e.
The counting result by is supplied to the red blood cell count correction unit 9g. In this correction unit 9g, the number of reticulocytes is corrected based on the reticulocyte count result and the red blood cell count result. Then, a signal D2 indicating the corrected number of reticulocytes, the number of red blood cells, and the number of platelets is output from the correction unit 9g and displayed by an appropriate display means (not shown) (cytogram display or the like).

As described above, according to the embodiment of the present invention, the light generated from the blood sample in the flow cell 11 is
The detection is performed by the white blood cell classification detector 6, the reticulocyte measurement detector 7, and the blood cell measurement detector 8, and these detection signals are supplied to the data processing unit 9. Then, in the data processing unit 9, the white blood cell classification value, reticulocyte count value, white blood cell count value, red blood cell count value, and platelet count value are calculated from the supplied detection signals, and each value is corrected by the calculated values. Then, accurate data is calculated. Therefore, white blood cell classification, reticulocyte measurement, and blood cell counting can be performed by one unit, and rapid blood analysis can be performed, and the detector, the amount of reagent and the amount of blood used are reduced, which saves space and resources. Can be achieved. further,
For example, it is possible to realize a blood analyzer that can calculate a highly accurate classification analysis result even when the number of white blood cells in blood is very large. In other words, the blood of leukemia patients has a very high number of white blood cells, and the appearance frequency of abnormal spheres is high.
It is often difficult to obtain an accurate classification ratio only with the normal white blood cell classification result. Therefore, for example, the number of white blood cells can be corrected from the white blood cell count value of the white blood cell counting unit 9c and the classification result of the white blood cell classification unit 9a, and a highly accurate white blood cell classification result can be obtained from the corrected number. Furthermore, it is possible to correct even when the number of white blood cells is small. Also,
Similarly, when the number of red blood cells is large, the number of reticulocytes can be obtained with high accuracy from the count value of the red blood cell counter 9d and the count value of the reticulocyte counter 9b.

FIG. 3 is a schematic view of the essential parts of a blood analyzer according to another embodiment of the present invention. In the example of FIG. 3, the blood sample solution 25 diluted and stained in the flow cell 11 is used.
A sheath flow is formed with the sheath liquid. And the light source 1
The cells are analyzed from several kinds of light information obtained by irradiating the cells in the flow cell 11 with the light from 2. In FIG. 3, the sample liquid distribution mechanism 5 including the reaction tank for the blood sample and the reagent is arranged so that the sample liquid 25 can be supplied to the flow cell 11. The light emitted from the light source 12 is focused by the condenser lens 13 on the detection area in the central portion of the flow cell 11 and focused. Scattered light and fluorescence from a fluorescent dye are generated from cells passing through this detection area. The forward scattered light is condensed by the condenser lens 14 and detected by the forward scattered light detector 18. Further, the side scattered light and the side fluorescent light are condensed by the condensing lens 24 in the right angle direction, and the side scattered light is filtered by the dichroic mirror 22.
It is supplied to the side scattered light detector 19 via 5. The intensity of the forward scattered light reflects the size of the cell, and the intensity of the side scattered light reflects the complexity of the intracellular structure, which is important information for flow-type cell analysis. The side fluorescent light from the condenser lens 24 passes through the dichroic mirror 22 and is then supplied to the dichroic mirror 23. Of the side fluorescence supplied to the dichroic mirror 23, green fluorescence is supplied to the green fluorescence detector 20 via the filter 16. Further, of the side fluorescence supplied to the dichroic mirror 23, red fluorescence is supplied to the red fluorescence detector 21 via the filter 17. These filters 15, 1
6 and 17 are selected to have optimum transmission characteristics depending on the wavelength of the light source used and the fluorescence wavelength characteristics of the fluorescent dye. Further, the detectors 18, 19, 20, and 21 are configured by, for example, a photomultiplier tube or a photodiode that converts an optical signal into an electric signal. Detector 18,
The detection signals from 19, 20, and 21 are sent to the data processing unit 90.
Is supplied to. FIG. 4 is a configuration diagram of the data processing unit 90. In FIG. 4, the green fluorescence detection signal G of the detector 20
Is supplied to the white blood cell sorting unit 90a, and the red fluorescence detection signal R of the detector 21 is supplied to the white blood cell sorting unit 90a and the reticulocyte counting unit 90b. Further, the forward scattered light detection signal F of the detector 18 is the white blood cell classification unit 90a, reticulocyte counting unit 90b, white blood cell counting unit 90c, red blood cell counting unit 90.
d, supplied to the platelet counting unit 90e. Further, the side scattered light detection signal S of the detector 19 is supplied to the white blood cell classification unit 90a. Then, the output signal of the white blood cell classification unit 90a and the output signal of the white blood cell counting unit 90c are supplied to the white blood cell count correction unit 90f. The output signal of the reticulocyte counting unit 90b, the output signal of the red blood cell counting unit 90d, and the output signal of the platelet counting unit 90e are supplied to the red blood cell count correcting unit 90g.

Next, in the example of FIG. 3, the blood analysis when a metachromatic fluorescent dye such as acridine orange is used as the fluorescent dye will be described. This acridine orange is commonly used for leukocyte classification and reticulocyte measurement. The light source 12 is an argon ion laser (wavelength 488 nm). Due to the nature of acridine orange, the white blood cell classification unit 90a uses the scattered light detection signals F and S from the detectors 18 and 19 and the fluorescence detection signals G and R from the detectors 20 and 21,
It becomes possible to classify white blood cells into five (lymphocytes, monocytes, neutrophils, eosinophils, basophils).

Further, the reticulocyte counting section 90b measures reticulocytes based on the red fluorescence detection signal R from the detector 21 and the forward scattered light detection signal F from the detector 18. Further, the forward scattered light detection signal F from the detector 18
The counting units 90c, 90d, and 90e count the numbers of white blood cells, red blood cells, and platelets, respectively. Then, the classification result by the white blood cell classification unit 9a and the counting result by the white blood cell counting unit 9c are supplied to the white blood cell count correction unit 9f. In the correction unit 9f, the number of white blood cells is corrected from the white blood cell classification result and the white blood cell count result, and an accurate white blood cell classification result is calculated from the corrected number. Then, a signal D1 indicating the corrected white blood cell count and the white blood cell classification result is output from the correction unit 9f and displayed by an appropriate display means (not shown). The counting results by the reticulocyte counting unit 9b, the red blood cell counting unit 9d, and the platelet counting unit 9e are supplied to the red blood cell count correcting unit 9g. In this correction unit 9g, the number of reticulocytes is corrected based on the reticulocyte count result and the red blood cell count result. And
A signal D2 indicating the corrected reticulocyte count, red blood cell count, and platelet count is output from the correction unit 9g and displayed by an appropriate display means (not shown).

Now, considering the number of detectors necessary for the conventional apparatus for performing leukocyte classification and leukocyte count analysis of only the leukocyte system, there are four detectors 18, 19, 20, 21. Further, the number of detectors required for the conventional apparatus for analyzing only the erythrocyte system such as reticulocyte count, red blood cell count, and platelet count is two detectors 18 and 21. Therefore, conventionally, the total number of detectors required for leukocyte analysis and erythrocyte analysis is six.
On the other hand, in the examples of FIGS. 3 and 4 according to the present invention, the detectors 18 and 21 are configured so as to be shared by the white blood cell system and the red blood cell system. The number of vessels is four, which is two less than that of the conventional analyzer.

As described above, according to another embodiment of the present invention, it is possible to obtain the same effect as that of the example of FIG. 1, and it is possible to obtain a fluorescent dye common to leukocyte classification and reticulocyte measurement.
For example, since acridine orange can be used, it is possible to use the same reagent and an equivalent detection system for leukocyte classification and reticulocyte measurement, and it is possible to analyze many types of blood with a simple structure. The obtained blood analyzer can be realized.

In the above-described embodiment, the blood analysis is carried out based on the scattered light and fluorescence of the sample in the flow cell. However, the detector for detecting the electric resistance value of the sample in the flow cell and this detector It is also possible to configure a blood analyzer including a white blood cell classification data, reticulocyte measurement data, and a data processing unit that calculates blood cell count data from the change in resistance value detected by. Even with such a configuration, it is possible to realize a blood analyzer capable of performing leukocyte classification, reticulocyte measurement, and blood cell counting by one unit. Further, if the data processing unit is configured to have a white blood cell count correction unit and a red blood cell count correction unit, highly accurate blood analysis results can be obtained.

[0026]

As described above, according to the present invention, the light irradiation means for irradiating cells with light in the detection region of the flow cell and the scattered light or fluorescence generated from the cells are detected, and the information for white blood cell classification is obtained. From the detector to detect, the detector to detect information for reticulocyte measurement, the detector to detect information for blood cell counting, the detector for white blood cell classification, the detector for reticulocyte measurement and the detector for blood cell counting Data processing means for calculating leukocyte classification data of the blood sample, reticulocyte measurement data, and blood cell measurement data based on the output detection signal. With this, white blood cell classification and
Realizes a blood analyzer that can perform reticulocyte measurement and blood cell counting, enables rapid blood analysis, and reduces the amount of detectors, reagents and blood used, and saves space and resources. can do. Furthermore, if the data processing means is configured to include a white blood cell count correction unit and a red blood cell count correction unit, not only is it possible to perform white blood cell classification, reticulocyte measurement, and blood cell counting by one unit,
It is possible to realize a blood analyzer that can calculate an analysis result such as a highly accurate classification ratio.

A resistance detector for detecting the electric resistance value of the sample liquid in the detection area of the flow cell, and white blood cell classification data, reticulocyte measurement data, and reticulocyte measurement data for the blood sample based on the detection signal output from the resistance detector. Data processing means for calculating blood cell measurement data. As a result, with one unit, white blood cell classification, reticulocyte measurement,
It is possible to realize a blood analyzer capable of performing blood cell counting, performing rapid blood analysis, reducing the amount of detectors, reagents and the amount of blood used, and saving space and resources. Furthermore, if the data processing means is configured to include a white blood cell count correction unit and a red blood cell count correction unit, not only can the white blood cell classification, reticulocyte measurement, and blood cell count be performed by one unit, but also highly accurate. It is possible to realize a blood analyzer that can calculate an analysis result such as a different classification ratio.

In the detection area of the flow cell, light irradiation means for irradiating cells with light, a detector for forward scattered light from cells, a detector for side scattered light from cells, and a green fluorescence from cells White blood cell classification based on the detection signals from the detectors, the red fluorescence from cells, the forward scattered light detector, the side scattered light detector, the green fluorescence detector and the red fluorescence detector. Data processing means for calculating data, reticulocyte measurement data and blood cell measurement data. This enables white blood cell classification, reticulocyte measurement, and blood cell counting to be performed with a single unit, enabling rapid blood analysis, and reducing the detector, reagent volume, and blood volume used, saving space and saving space. It is possible to realize a blood analyzer in which recycling is achieved. Furthermore, if the data processing means is configured to include a white blood cell count correction unit and a red blood cell count correction unit, not only can the white blood cell classification, reticulocyte measurement, and blood cell count be performed by one unit, but also highly accurate. It is possible to realize a blood analyzer that can calculate an analysis result such as a different classification ratio.

[Brief description of drawings]

FIG. 1 is a schematic configuration diagram of an embodiment of the present invention.

FIG. 2 is a schematic diagram of a sample liquid distribution mechanism in the example of FIG.

FIG. 3 is a schematic configuration diagram of another embodiment of the present invention.

FIG. 4 is a configuration diagram of a data processing unit in the example of FIG.

[Explanation of symbols]

 1 sample suction mechanism 2, 3, 4 dilution staining mechanism 5 sample liquid distribution mechanism 6, 7, 8 detector 9, 90 data processing unit 9a, 90a leukocyte classification unit 9b, 90b reticulocyte counting unit 9c, 90c leukocyte counting unit 9d , 90d Red blood cell counting unit 9e, 90e Platelet counting unit 9f, 90f White blood cell number correcting unit 9g, 90g Red blood cell number correcting unit 11 Flow cell 12 Light source 13, 14, 24 Condensing lens 15, 16, 17 Filter 18 Forward scattered light detector 19 Side scattered light detector 20 Green fluorescence detector 21 Red fluorescence detector 22, 23 Dichroic mirror 25 Blood sample solution 26 Reaction tank

Claims (6)

[Claims] 1. A reaction tank for mixing and reacting a blood sample solution and a diluted staining reagent, a flow cell capable of passing cells in the sample solution one by one through a detection region, and the sample solution. A sample liquid distribution mechanism for supplying the cells from the reaction tank to the flow cell, a light irradiation means for irradiating cells with light in the detection region of the flow cell, and scattered light or fluorescence generated from the cells is detected to classify leukocytes. Detector for detecting information on cells, a detector for detecting scattered light or fluorescence generated from cells, detecting information for measuring reticulocytes, and detector for detecting scattered light or electric resistance generated on cells for blood cell counting Of the blood sample based on the detection signals output from the detector for detecting information for blood cell classification, the detector for white blood cell classification, the detector for reticulocyte measurement and the detector for blood cell counting. Sphere classification data, blood analysis apparatus characterized by comprising: a data processing means for calculating a reticulocyte measurement data and the blood measurement data. 2. The blood analyzer according to claim 1, wherein
The data processing means comprises a white blood cell classification unit, a reticulocyte counting unit, a white blood cell counting unit, a red blood cell counting unit, a platelet counting unit, a white blood cell classification unit and a white blood cell classification ratio according to output signals from the white blood cell classification unit and the white blood cell counting unit. A blood analyzer comprising: a white blood cell count correction unit that corrects the red blood cell count correction unit; and a red blood cell count correction unit that corrects at least the red blood cell count according to output signals from the reticulocyte count unit, the red blood cell count unit, and the platelet count unit. .. 3. A reaction tank for mixing and reacting a blood sample solution and a diluted staining reagent, a flow cell capable of passing cells in the sample solution one by one through a detection region, and the sample solution. From the reaction tank, a sample liquid distribution mechanism that supplies the flow cell to the flow cell, a resistance detector that detects the electrical resistance value of the sample liquid in the detection region of the flow cell, and a detection signal output from the resistance detector based on And a data processing unit for calculating leukocyte classification data of a blood sample, reticulocyte measurement data, and blood cell measurement data. 4. The blood analyzer according to claim 3, wherein
The data processing means comprises a white blood cell classification unit, a reticulocyte counting unit, a white blood cell counting unit, a red blood cell counting unit, a platelet counting unit, a white blood cell classification unit and a white blood cell classification ratio according to output signals from the white blood cell classification unit and the white blood cell counting unit. A blood analyzer comprising: a white blood cell count correction unit that corrects the red blood cell count correction unit; and a red blood cell count correction unit that corrects at least the red blood cell count according to output signals from the reticulocyte count unit, the red blood cell count unit, and the platelet count unit. .. 5. A reaction tank for mixing and reacting a blood sample solution and a diluted staining reagent, a flow cell capable of passing cells in the sample solution one by one through a detection region, and the sample solution. A sample liquid distribution mechanism for supplying the flow cell from the reaction tank to the flow cell, a light irradiation means for irradiating cells with light in the detection region of the flow cell, a detector for detecting forward scattered light generated from the cells, Detector for detecting side-scattered light generated from cells, detector for detecting green fluorescence generated from cells, detector for detecting red fluorescence generated from cells, the above-mentioned forward scattered light detector, side-scattering Calculate the white blood cell classification data, reticulocyte measurement data and blood cell measurement data of the blood sample based on the detection signals output from the photo detector, green fluorescence detector and red fluorescence detector. Blood analysis apparatus characterized by comprising: a data processing unit, the for. 6. The blood analyzer according to claim 5, wherein
The data processing means comprises a white blood cell classification unit, a reticulocyte counting unit, a white blood cell counting unit, a red blood cell counting unit, a platelet counting unit, a white blood cell classification unit and a white blood cell classification ratio according to output signals from the white blood cell classification unit and the white blood cell counting unit. And a red blood cell count correcting unit that corrects at least the red blood cell count according to the output signals from the reticulocyte counting unit, the red blood cell counting unit, and the platelet counting unit. The output signals from the photodetector, the side scattered light detector, the green fluorescence detector and the red fluorescence detector are supplied, and the output signals from the forward scattered light detector and the red fluorescence detector are supplied to the reticulocyte counting section. Supplied, white blood cell counter,
A blood analyzer characterized in that an output signal from a forward scattered light detector is supplied to the red blood cell counting section and the platelet counting section.