JPH06189650A - Method for improving conception rate - Google Patents
Method for improving conception rateInfo
- Publication number
- JPH06189650A JPH06189650A JP14251593A JP14251593A JPH06189650A JP H06189650 A JPH06189650 A JP H06189650A JP 14251593 A JP14251593 A JP 14251593A JP 14251593 A JP14251593 A JP 14251593A JP H06189650 A JPH06189650 A JP H06189650A
- Authority
- JP
- Japan
- Prior art keywords
- egg
- sperm
- fertilized
- magnetic force
- eggs
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims description 23
- 230000035800 maturation Effects 0.000 claims abstract description 6
- 230000009303 sperm storage Effects 0.000 claims description 6
- 239000002657 fibrous material Substances 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims 1
- 235000013601 eggs Nutrition 0.000 abstract description 65
- 230000004083 survival effect Effects 0.000 abstract description 11
- 230000004720 fertilization Effects 0.000 abstract description 10
- 210000000582 semen Anatomy 0.000 abstract description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 8
- 241001465754 Metazoa Species 0.000 abstract description 7
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 6
- 239000003085 diluting agent Substances 0.000 abstract description 4
- 239000007788 liquid Substances 0.000 abstract description 4
- 239000001963 growth medium Substances 0.000 abstract description 3
- 229920000742 Cotton Polymers 0.000 abstract description 2
- 230000003247 decreasing effect Effects 0.000 abstract description 2
- 239000000835 fiber Substances 0.000 abstract description 2
- 238000007710 freezing Methods 0.000 abstract description 2
- 230000008014 freezing Effects 0.000 abstract description 2
- 238000004140 cleaning Methods 0.000 abstract 1
- 238000004321 preservation Methods 0.000 abstract 1
- 238000003860 storage Methods 0.000 description 10
- 238000002054 transplantation Methods 0.000 description 8
- 239000010902 straw Substances 0.000 description 6
- 241000283690 Bos taurus Species 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 230000001678 irradiating effect Effects 0.000 description 5
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- 230000002068 genetic effect Effects 0.000 description 4
- 244000144972 livestock Species 0.000 description 4
- 244000309464 bull Species 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 210000004681 ovum Anatomy 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 241000124008 Mammalia Species 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000009027 insemination Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000010257 thawing Methods 0.000 description 2
- 210000004291 uterus Anatomy 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241000700198 Cavia Species 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000283086 Equidae Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 239000010419 fine particle Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 210000005239 tubule Anatomy 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は、哺乳動物を始めとする
多くの動物の人工授精における受胎率向上方法に関し、
さらに詳細には、体外人工授精における卵子の受精率や
受精卵および精子の生存率を高める受胎率向上方法に関
する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for improving the conception rate in artificial insemination of many animals including mammals,
More specifically, the present invention relates to a method of improving the conception rate for increasing the fertilization rate of eggs and the survival rate of fertilized eggs and sperms during in vitro artificial insemination.
【０００２】[0002]
【従来の技術】優れた遺伝的形質を有する雌畜の卵子
を、同じく優れた遺伝的形質を有する雄畜の精子で受精
させ、これを他の雌畜（レシピエント）の子宮に移植す
る受精卵移植の技術は、効率良く、優れた家畜や実験動
物を産出する技術として注目を集めている。2. Description of the Related Art Fertilization in which an ovum of a female livestock having an excellent genetic trait is fertilized with a sperm of a male livestock also having an excellent genetic trait, and this is transplanted to the uterus of another female livestock (recipient). The technique of egg transplantation has been attracting attention as a technique for efficiently producing excellent domestic animals and experimental animals.
【０００３】この受精卵移植の技術においては、優れた
遺伝的形質を受け継いだ受精卵を有効にレシピエントの
子宮中で発育せしめることが経済的に重要であり、その
ためには、受胎率、受精率等を向上せしめることが重要
とされていた。In this fertilized egg transplantation technique, it is economically important to effectively develop fertilized eggs that have inherited excellent genetic traits in the recipient's uterus. It was considered important to improve the rate.
【０００４】[0004]
【発明が解決しようとする課題】しかし、受精卵移植に
おいては、受胎率が５０％程度と低い欠点があり、受胎
率向上のためのなんらかの解決法が求められていた。However, fertilized egg transplantation has a drawback that the conception rate is as low as about 50%, and some solution has been required to improve the conception rate.
【０００５】[0005]
【課題を解決するための手段】本発明者は、受精卵移植
で利用される、精子、卵子および受精卵について、その
生存性、成熟度、受精率等を向上させ、その結果として
受胎率を向上させるべく鋭意研究を行った結果、これら
に磁力を作用させることによりその目的が達成できるこ
とを見出し、本発明を完成した。The present inventor has improved the viability, maturity, fertilization rate, etc. of sperm, eggs and fertilized eggs used in fertilized egg transplantation, and as a result, the conception rate can be improved. As a result of earnest research to improve it, they found that the objective can be achieved by applying a magnetic force to them, and completed the present invention.
【０００６】すなわち、本発明は、雄の精子、雌の卵子
またはこれらから得られる受精卵に磁力を作用させるこ
とを特徴とする受胎率の向上方法である。That is, the present invention is a method for improving the conception rate, which comprises applying a magnetic force to a male sperm, a female egg, or a fertilized egg obtained from these.
【０００７】本発明方法を実施するには、受精卵移植の
各工程において得られる精子、卵子および受精卵のいず
れかに、一定の強度の磁力を作用させれば良い。In order to carry out the method of the present invention, a magnetic force of a certain strength may be applied to any of the sperm, egg and fertilized egg obtained in each step of the fertilized egg transplantation.
【０００８】磁力を作用させる方法としては、精子、卵
子または受精卵の一定の成熟段階において短時間磁力を
照射しても良く、また、これらの保存中において継続的
に磁石と共存せしめても良い。As a method of applying a magnetic force, a magnetic force may be applied for a short time at a certain maturation stage of a sperm, an egg or a fertilized egg, or they may be allowed to coexist continuously with a magnet during storage. .
【０００９】磁力を照射する場合の磁力強度、照射時間
は、動物の種類によって若干異なり、実験的に最適条件
を見出だす必要があるが、一般には、強度が１００〜１
００００ガウス程度、好ましくは１０００〜２０００ガ
ウス程度の磁力を、１分間〜１０時間程度、好ましくは
３〜３０分間程度照射すれば良い。The intensity of magnetic force and the duration of irradiation when magnetic force is applied differ slightly depending on the type of animal, and it is necessary to experimentally find the optimum conditions. Generally, the intensity is 100 to 1
A magnetic force of about 0000 Gauss, preferably about 1000 to 2000 Gauss may be applied for about 1 minute to 10 hours, preferably about 3 to 30 minutes.
【００１０】このような磁力の照射は、所定の強度の磁
力を得ることができるものであれば、永久磁石、電磁石
の何れによっても良い。Irradiation of such a magnetic force may be performed with a permanent magnet or an electromagnet as long as a magnetic force of a predetermined intensity can be obtained.
【００１１】より具体的に、精子に磁力を照射する場合
は、雄から精子を取得した後、すぐに磁力を照射し、次
いで凍結保存すればよく、そうすることにより、解凍後
の生存率は、磁力を照射しないものに比べ９％程度、ま
た、受胎率も５％程度向上する。 この場合の磁力の照
射時間は、通常３〜１５分程度の短時間であっても良
い。More specifically, in the case of irradiating sperm with a magnetic force, after obtaining sperm from a male, the sperm may be immediately irradiated with a magnetic force and then cryopreserved, whereby the survival rate after thawing is improved. Approximately 9%, and the conception rate is also improved by approximately 5% compared to those without magnetic irradiation. In this case, the irradiation time of the magnetic force may be a short time, usually about 3 to 15 minutes.
【００１２】また、未成熟卵に磁力を照射する場合は、
雌から卵子を採取後、その培養途中の何れかの段階で磁
力を照射すればよく、そうすることにより、成熟卵の取
得率が１５％程度向上し、また、受精率は１１％程度向
上する。When magnetically irradiating an immature egg,
After collecting the ova from the female, the magnetic force may be applied at any stage during the culture, which improves the acquisition rate of mature eggs by about 15% and the fertilization rate by about 11%. .
【００１３】更に、雄の精子と雌の卵子から得られる受
精卵は、好ましくは、受精卵採取直後に、３分間〜５時
間程度以上磁力を照射し、凍結保存すればよい。そうす
ることにより、解凍後の生存率は、磁力を照射しないも
のに比べ、生存率で１７％以上向上し、また、受胎率も
１８％程度向上する。 この理由は、受精卵の延命効果
と受精卵中に存在する未発育の受精卵が磁力照射後の培
養により、十分発育するためと考えられる。Further, the fertilized egg obtained from the male sperm and the female egg may be subjected to magnetic force irradiation for 3 minutes to 5 hours or more immediately after the fertilized egg is collected, and may be frozen and stored. By doing so, the survival rate after thawing is improved by 17% or more in the survival rate and the conception rate is also improved by about 18% as compared with the case where the magnetic force is not applied. It is considered that this is because the life-prolonging effect of the fertilized egg and the undeveloped fertilized egg present in the fertilized egg are sufficiently developed by the culture after the magnetic irradiation.
【００１４】一方、精子、卵子または受精卵の保存中に
おいて継続的に磁石と共存せしめる方法としては、これ
らの保存容器等に微細なを充填、作用させる方法が挙げ
られる。 例えば、精子は一般にストローのような細管
に取り、これを凍結または凍結乾燥して保存するのであ
るから、この細管中に微細磁石を充填すれば保存期間中
に渡って精子に磁力を作用させることが可能になる。On the other hand, as a method of allowing a sperm, an egg, or a fertilized egg to coexist continuously with a magnet during storage, there is a method of filling and storing these storage containers with fine particles. For example, sperm are generally stored in a thin tube such as a straw and then frozen or lyophilized for storage.Therefore, if a fine magnet is filled in the thin tube, magnetic force will be applied to the sperm during the storage period. Will be possible.
【００１５】上記方法において使用しうる精子保存用細
管の例を図１および図２に示す。各図において、１は細
管、２は微細磁石、３は繊維質である。 １は、両端が
開放になっており、図の向かって左端から精液を吸い込
み、その後、同端をストローパウダー等で封管する。
また、右端の繊維質は綿等で構成され、精液が流出する
ことを防ぐものである。Examples of sperm storage tubules that can be used in the above method are shown in FIGS. 1 and 2. In each figure, 1 is a thin tube, 2 is a fine magnet, and 3 is fibrous. In No. 1, both ends are open, and semen is sucked in from the left end in the figure, and then the end is sealed with straw powder or the like.
The fiber at the right end is made of cotton or the like, and prevents semen from flowing out.
【００１６】磁石は、精液の流動の妨げにならないよう
な微細なものであることが好ましく、その充填位置は、
図１のように繊維質と別であっても、また、図２のよう
に繊維質の中であっても良い。 また、一ヵ所であって
も、複数の位置に充填しても良い。 更に、封管に用い
るストローパウダーに微細磁石を混合し、封管と同時に
磁石が充填できるようにしても良い。The magnet is preferably a fine one that does not hinder the flow of semen, and the filling position is
It may be different from the fibrous material as shown in FIG. 1 or may be in the fibrous material as shown in FIG. Further, it may be filled at one location or at a plurality of locations. Furthermore, the straw powder used for the sealed tube may be mixed with a fine magnet so that the magnet can be filled simultaneously with the sealed tube.
【００１７】本発明方法により、受精卵の受胎率を向上
させるためには、上記の、精子、卵子または受精卵に磁
力を作用させる以外は、従来行われていた方法で受精卵
移植を行えば良い。 すなわち、雄からの精子の取得、
保存、雌からの卵子の取得、培養、受精、受精卵の保
存、移植等は従来公知の方法に従って実施することがで
きる。In order to improve the conception rate of fertilized eggs by the method of the present invention, fertilized egg transplantation can be carried out by any of the conventional methods except that a magnetic force is applied to the sperm, egg or fertilized egg. good. That is, getting sperm from a male,
Storage, acquisition of eggs from females, culture, fertilization, storage of fertilized eggs, transplantation and the like can be carried out according to conventionally known methods.
【００１８】本発明方法は、哺乳動物、例えば豚、牛、
馬、羊、ヤギ等の家畜や、ウサギ、ラット、マウス、モ
ルモット、ハムスター等の実験動物に広く利用できるも
のである。また、これのみに留まらず、各種魚類や各種
鳥類等にも適用が可能なものである。The method of the present invention can be applied to mammals such as pigs, cows,
It is widely applicable to domestic animals such as horses, sheep and goats, and experimental animals such as rabbits, rats, mice, guinea pigs and hamsters. Moreover, the present invention is not limited to this, and can be applied to various kinds of fish and various kinds of birds.
【００１９】[0019]
【実施例】次に、実施例を挙げ、本発明を更に詳しく説
明するが、本発明はこれら実施例になんら制約されるも
のではない。EXAMPLES Next, the present invention will be described in more detail with reference to examples, but the present invention is not limited to these examples.
【００２０】実 施 例 １ 精子に対する磁力照射：雄牛から、常法にしたがって精
液を採取し、希釈液（ＮＦ−３ ；武田薬品（株）製）
を用い、公知の方法に従い２次に分けて希釈した（一次
希釈液：原精液＝７.５：１、二次希釈液：一次希釈精
液＝７.５：１）。 なお、一次希釈は３０℃で、二次希
釈は４℃で行った（３０℃から４℃へは６０〜９０分か
けて減温した）。 希釈後、希釈液の３ｍｌを１５ｍｌ
の試験管に取り、Ｎ−Ｓの磁極で挟み、１６００ガウス
で１５分間磁力を照射した。 磁力を照射後、０.５ｍｌ
ストローに取り、液体窒素（−１９６℃）にて凍結し
た。 凍結してから３カ月経過後、解凍し、生存してい
る精子の数を調べ、生存率を求めた。 なお、希釈以後
の操作はすべて４℃の恒温室中で行った。Example 1 Magnetic irradiation of sperm: Semen was collected from a bull according to a conventional method and diluted (NF-3; manufactured by Takeda Pharmaceutical Co., Ltd.).
Was diluted into two parts in accordance with a known method (primary diluent: original semen = 7.5: 1, secondary diluent: primary diluted semen = 7.5: 1). The primary dilution was performed at 30 ° C and the secondary dilution was performed at 4 ° C (the temperature was decreased from 30 ° C to 4 ° C over 60 to 90 minutes). After dilution, add 3 ml of diluent to 15 ml
It was taken in a test tube of No. 2, sandwiched between N-S magnetic poles, and magnetic force was applied for 15 minutes at 1600 gauss. After irradiation with magnetic force, 0.5 ml
It was taken in a straw and frozen in liquid nitrogen (-196 ° C). After 3 months from freezing, the cells were thawed, the number of surviving sperms was examined, and the survival rate was calculated. All the operations after the dilution were performed in a constant temperature room at 4 ° C.
【００２１】また、凍結保存したこれらの精子を用い
て、雌牛の成熟卵子を人工授精させ、受精２カ月後に直
腸検査法により、その受胎を調べ受胎率を求めた。これ
らの結果を第１表に示す。[0021] Using these cryopreserved spermatozoa, mature eggs of cows were artificially inseminated, and two months after fertilization, the conception was examined by rectal examination to determine the conception rate. The results are shown in Table 1.
【００２２】 [0022]
【００２３】この結果から明らかなように、精子に磁力
を照射することによって、精子の生存率、これを用いた
場合の受胎率とも向上した。As is clear from these results, by irradiating the sperm with a magnetic force, the survival rate of sperm and the conception rate when using it were improved.
【００２４】実 施 例 ２ 卵子（未成熟卵）に対する磁力照射：雌牛から、子宮洗
浄により卵子を採取した。 これを、培養液（ＴＣＭ−
１９９；ギブコ社製）をいれた試験管に移し、３９℃の
ふ卵器で２４〜４８時間培養し成熟させた。 培養開始
３時間後に、試験管を磁石のＮ−Ｓ極で挟むようにし
て、１６００ガウスの磁力を８時間照射した。培養終了
後、顕微鏡下で成熟卵数を調べ、成熟卵取得率をそれぞ
れ求めた。Example 2 Magnetic irradiation of eggs (immature eggs): Eggs were collected from cows by uterine washing. This is a culture solution (TCM-
199; manufactured by Gibco) was transferred to the inserted test tube and cultured in an incubator at 39 ° C. for 24 to 48 hours to be matured. After 3 hours from the start of the culture, the test tube was sandwiched between the N and S poles of the magnet and irradiated with a magnetic force of 1600 gauss for 8 hours. After the culture was completed, the number of mature eggs was examined under a microscope, and the mature egg acquisition rate was determined.
【００２５】培養後の卵子を、雄牛の精子を用いて、受
精させ、更に培養液（ＴＣＭ−１９９；ギブコ社製）で
７日間培養を行なった。培養終了後、顕微鏡下で受精卵
数を調べ、受精率を求めた。これらの結果を第２表に示
す。After culturing, the ova were fertilized using bull sperm, and further cultivated for 7 days in a culture medium (TCM-199; Gibco). After the culture was completed, the number of fertilized eggs was examined under a microscope to determine the fertilization rate. The results are shown in Table 2.
【００２６】 [0026]
【００２７】この結果から明らかなように、卵子に磁力
を照射することによって、卵子の成熟数および受精率は
ともに向上した。As is clear from these results, by irradiating the eggs with magnetic force, both the maturation number of eggs and the fertilization rate were improved.
【００２８】実 施 例 ３ 受精卵に対する磁力照射：受胎後７日目の受精卵を、子
宮洗浄法により雌牛から採取した。 これを、培養液
（ＴＣＭ−１９９；ギブコ社製）と共に試験管に移し、
３９℃のふ卵器で４８時間培養した。 培養開始３時間
後に、この試験管を磁石のＳ−Ｎ極の間に挟み、１６０
０ガウスの磁力を８時間照射した。 培養終了後、この
受精卵を液体窒素を用いて凍結し、３カ月間保存した。Example 3 Magnetic irradiation of fertilized eggs: Fertilized eggs 7 days after conception were collected from cows by the uterine washing method. This was transferred to a test tube together with a culture solution (TCM-199; manufactured by Gibco),
The cells were cultured in an incubator at 39 ° C for 48 hours. After 3 hours from the start of culturing, the test tube was sandwiched between the S and N poles of the magnet, and 160
A magnetic force of 0 gauss was applied for 8 hours. After the completion of the culture, the fertilized eggs were frozen with liquid nitrogen and stored for 3 months.
【００２９】凍結保存後、この受精卵を解凍し、顕微鏡
下で生存卵の数を調べた。 生存卵の判定に当たって
は、日齢にあった胚のステージにあるか、水泡や突
出した細胞があるかどうか、受精卵の色調が異常に明
るいものや暗いものがあるかどうか、細胞が集団とし
てよくまとまり、輪郭が鮮明であるかどうか、透明帯
にキズがないかどうか等を考慮した。 また、この受精
卵を雌牛に移植し、人工受精２カ月後に直腸検査法でそ
の受胎を調べ、受胎率を求めた。この結果を第３表に示
す。After frozen storage, the fertilized eggs were thawed and the number of surviving eggs was examined under a microscope. In determining the number of surviving eggs, the cells are in the population as a group at the stage of the embryo at the age of day, whether there are blisters or protruding cells, whether the fertilized eggs have abnormally bright or dark colors. It was well organized and the contours were clear, and there were no scratches on the transparent zone. Further, this fertilized egg was transplanted to a cow, and two months after artificial fertilization, the conception was examined by a rectal test method, and the conception rate was calculated. The results are shown in Table 3.
【００３０】 [0030]
【００３１】この結果から明らかなように、受精卵に磁
力を照射することによって、冷凍保存後の生存率および
受胎率はともに向上した。As is clear from these results, by irradiating fertilized eggs with magnetic force, both the survival rate and the conception rate after frozen storage were improved.
【００３２】実 施 例 ４ 磁石を用いた精子の保存：雄牛から、常法にしたがって
精液を採取し、実施例１の方法に従い希釈した。 一
方、磁力１８００ガウスの磁石を砕いて得た径２ｍｍ以
下の磁石粒ｍｇを図１に示すような精子保存用細管
（０.５ｍｌストロー）に充填し、これに上記希釈精液
を入れ、一端を封管した。この細管を液体窒素（−１９
６℃）で凍結保存した。 保存１ヵ月後、これを３８℃
で解凍し、３時間後の生存精子の数を調べ、生存率を求
めた。この結果を第４表に示す。Example 4 Storage of Sperm Using a Magnet: Semen was collected from a bull according to a conventional method and diluted according to the method of Example 1. On the other hand, magnet particles mg having a diameter of 2 mm or less obtained by crushing a magnet with a magnetic force of 1800 gauss were filled in a sperm storage thin tube (0.5 ml straw) as shown in FIG. I sealed it. This thin tube is filled with liquid nitrogen (-19
It was stored frozen at 6 ° C. One month after storage, this is 38 ℃
The cells were thawed and the number of surviving sperms after 3 hours was examined to determine the survival rate. The results are shown in Table 4.
【００３３】 [0033]
【００３４】この結果から明らかなように、精子に磁力
を共存せしめることによって、精子の生存率が向上し
た。As is clear from these results, the coexistence of magnetic force in sperm improved the survival rate of sperm.
【００３５】[0035]
【発明の効果】本発明によれば、簡単な処理により、受
精卵移植法における凍結保存により低下する精子や受精
卵の生存率を引き上げることが可能となり、また、未成
熟卵子の成熟を促進することも可能となるものである。
従って、優れた遺伝的形質を有する動物を効率良く、経
済的に生産することが可能になるので、畜産等の分野に
おいて極めて有利なものである。INDUSTRIAL APPLICABILITY According to the present invention, it is possible to increase the survival rate of spermatozoa and fertilized eggs that are reduced by cryopreservation in the fertilized egg transplantation method by a simple treatment, and promote the maturation of immature eggs. It is also possible.
Therefore, it becomes possible to efficiently and economically produce an animal having an excellent genetic trait, which is extremely advantageous in the field of livestock production and the like.
【図１】 本発明精子保存用細管の１例の縦断面を示す
図面。FIG. 1 is a drawing showing a vertical cross section of an example of a sperm storage thin tube of the present invention.
【図２】 本発明精子保存用細管の別の例の縦断面図を
示す図面。FIG. 2 is a drawing showing a longitudinal sectional view of another example of the thin tube for sperm storage of the present invention.
１ … … 細管（ストロー） ２ … … 微細磁石 ３ … … 繊維質 以 上 1 ……… Thin tube (straw) 2 ……… Micro magnet 3 ……… Fiber material and above
Claims (5)
られる受精卵に磁力を作用させることを特徴とする受胎
率の向上方法。1. A method for improving the conception rate, which comprises applying a magnetic force to a male sperm, a female egg, or a fertilized egg obtained from these.
し、これに磁力を照射することを特徴とする請求項１記
載の受胎率の向上方法。2. The method for improving the conception rate according to claim 1, wherein the egg fertilized in the female body is taken out to the outside and magnetic force is applied to the egg.
する卵子の成熟促進方法。3. A method for promoting the maturation of an egg, which comprises applying a magnetic force to the female egg.
とする精子の活性向上方法。4. A method for improving sperm activity, which comprises applying a magnetic force to male sperm.
したことを特徴とする精子保存用細管。5. A thin tube for sperm storage, characterized in that the thin tube is filled with a fine magnet and a fibrous material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP14251593A JPH06189650A (en) | 1992-10-27 | 1993-05-24 | Method for improving conception rate |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP4-310805 | 1992-10-27 | ||
| JP31080592 | 1992-10-27 | ||
| JP14251593A JPH06189650A (en) | 1992-10-27 | 1993-05-24 | Method for improving conception rate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH06189650A true JPH06189650A (en) | 1994-07-12 |
Family
ID=26474495
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14251593A Pending JPH06189650A (en) | 1992-10-27 | 1993-05-24 | Method for improving conception rate |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH06189650A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003523198A (en) * | 2000-02-15 | 2003-08-05 | オボ バイオサイエンシーズ， インコーポレイテッド | In ovo activation of avian eggs in a shell |
| US9962350B2 (en) | 2014-07-11 | 2018-05-08 | Sbi Pharmaceuticals Co., Ltd. | Agent for improving normal development rate of fertilized eggs |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5075872A (en) * | 1973-10-04 | 1975-06-21 |
-
1993
- 1993-05-24 JP JP14251593A patent/JPH06189650A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5075872A (en) * | 1973-10-04 | 1975-06-21 |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003523198A (en) * | 2000-02-15 | 2003-08-05 | オボ バイオサイエンシーズ， インコーポレイテッド | In ovo activation of avian eggs in a shell |
| US9962350B2 (en) | 2014-07-11 | 2018-05-08 | Sbi Pharmaceuticals Co., Ltd. | Agent for improving normal development rate of fertilized eggs |
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