JPH0318455B2 - - Google Patents
Info
- Publication number
- JPH0318455B2 JPH0318455B2 JP58072247A JP7224783A JPH0318455B2 JP H0318455 B2 JPH0318455 B2 JP H0318455B2 JP 58072247 A JP58072247 A JP 58072247A JP 7224783 A JP7224783 A JP 7224783A JP H0318455 B2 JPH0318455 B2 JP H0318455B2
- Authority
- JP
- Japan
- Prior art keywords
- electrode
- cartridge
- blood
- blood collection
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 210000004369 blood Anatomy 0.000 claims description 25
- 239000008280 blood Substances 0.000 claims description 25
- 238000005259 measurement Methods 0.000 claims description 14
- 239000012528 membrane Substances 0.000 description 11
- 239000003792 electrolyte Substances 0.000 description 7
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 5
- 229940088598 enzyme Drugs 0.000 description 5
- 238000000034 method Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 229940075397 calomel Drugs 0.000 description 3
- ZOMNIUBKTOKEHS-UHFFFAOYSA-L dimercury dichloride Chemical compound Cl[Hg][Hg]Cl ZOMNIUBKTOKEHS-UHFFFAOYSA-L 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 2
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 238000011088 calibration curve Methods 0.000 description 2
- 239000000460 chlorine Substances 0.000 description 2
- 229910052801 chlorine Inorganic materials 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 108010093096 Immobilized Enzymes Proteins 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 108010042687 Pyruvate Oxidase Proteins 0.000 description 1
- 108010092464 Urate Oxidase Proteins 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229940116332 glucose oxidase Drugs 0.000 description 1
- 235000019420 glucose oxidase Nutrition 0.000 description 1
- WABPQHHGFIMREM-UHFFFAOYSA-N lead(0) Chemical compound [Pb] WABPQHHGFIMREM-UHFFFAOYSA-N 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 235000011151 potassium sulphates Nutrition 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
Landscapes
- Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
Description
【発明の詳細な説明】
本発明は積層電極カートリツジを用いた多項目
同時測定装置に関するものである。DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a multi-item simultaneous measurement device using a laminated electrode cartridge.
緊急検査では、刻々と変化する生体の各種の物
質濃度を迅速、正確かつ簡便にとらえることを要
求される。それらの物質の実際の測定には、ガラ
ス膜電極、固体膜電極、液膜電極、ガス透過性膜
電極、酸素及び過酸化水素電極等、各種イオンま
たは分子選択性電極を利用した自動分析装置が多
く用いられている。しかしその多くは、血液試料
から血漿または血清を分離してはじめて利用可能
になるもので一部に利用されている全血のまま利
用できる装置の場合でも、採血の際に測定が進行
するようなものはこれまでのところ見られていな
い。 Emergency testing requires rapid, accurate, and simple measurement of the ever-changing concentrations of various substances in living organisms. The actual measurement of these substances requires automatic analyzers that use various ion- or molecular-selective electrodes, such as glass membrane electrodes, solid membrane electrodes, liquid membrane electrodes, gas-permeable membrane electrodes, oxygen and hydrogen peroxide electrodes, etc. It is often used. However, in many cases, it can only be used after separating plasma or serum from a blood sample, and even in the case of some devices that can use whole blood, the measurement proceeds during blood collection. Nothing has been seen so far.
一方、生体から採取する血液などの試料中の物
質には、採取直後から経時的に変化を続けるもの
があり、これらの物質を測定して緊急検査におけ
る臨床情報として利用するためには、採取直後に
速やかに測定し終えるか、または、一気に氷冷し
て、測定が実施されるまで保存する必要がある。
更には、血漿中のイオン化カルシウムのように、
採血直後からはじまる試料炭酸ガス含量の変化に
伴つて生ずるPHの変化によつて大きく測定値が変
化してしまうものもあり、従来からの各種電極に
よる自動分析装置では問題を残している。 On the other hand, some substances in samples such as blood collected from living organisms continue to change over time immediately after collection, and in order to measure these substances and use them as clinical information in emergency tests, it is necessary to It is necessary to finish the measurement as soon as possible, or to cool it on ice all at once and store it until the measurement is performed.
Furthermore, like ionized calcium in plasma,
Measured values may change significantly due to changes in pH that occur due to changes in sample carbon dioxide content immediately after blood collection, and this remains a problem with conventional automatic analyzers using various electrodes.
本発明は、前述のごとき従来の装置および方法
の問題点の改善を目的としてなされたもので、患
者から検体（例えば血液）を採取する際、採取の
過程で一部の臨床的情報を検体から取り出し、採
取した検体は、そのまま他の情報を得るための試
料として用いることができ、操作も容易で迅速、
正確に各種測定を行える多項目同時測定装置を提
供することを目指している。 The present invention was made with the aim of improving the problems of conventional devices and methods as described above. When a specimen (for example, blood) is collected from a patient, some clinical information is collected from the specimen during the collection process. The removed and collected specimen can be used as it is as a sample to obtain other information, and the operation is easy and quick.
We aim to provide a multi-item simultaneous measurement device that can accurately perform various measurements.
本発明によれば、血液などが患者の血管から直
接容器内に流入して、温度、PHなどがまだ生体内
とほぼ同じレベルにある時点で測定し終えるため
に、より確かな生体情報として取り出すことがで
き、しかも、検体は、この過程では全く消費する
ことなく、他の生体情報の検査の試料として利用
できる。また、情報を取り出すまでの時間が著し
く短かいために、臨床的有用性を増すうえで著し
いものがある。 According to the present invention, since blood flows directly into the container from the patient's blood vessels and the measurement is completed when the temperature, pH, etc. are still at approximately the same level as in the body, more reliable biological information is extracted. Moreover, the specimen can be used as a sample for testing other biological information without being consumed at all during this process. Additionally, the time to retrieve information is significantly shorter, which significantly increases clinical utility.
以下、図面を参照して本発明の実施例を説明す
る。 Embodiments of the present invention will be described below with reference to the drawings.
第１図は、本発明による積層電極カートリツジ
の一実施例を示す縦断面図である。カロメル参照
電極用電解液注入孔１は計測機本体の参照電極用
電解液側に接続され、全体を構成する各電極はそ
れぞれの変換可能である連続して隣接して配設さ
れたブロツクより成り、カロメル参照電極２、塩
素電極３、カルシウム電極４、カリウム電極５、
酸素又は過酸化水素用酵素膜電極６、ナトリウム
電極７などを配し、それらはターミナル８及び９
を介して計測機本体の電位差計または電流計へと
接続され、カロメル参照電極には多孔質板１０
を、酵素膜電極には酵素固定化膜１１を設け、試
料孔１２の一方が採血針接続部分１３に他方が採
血注射器接続部分１４に連通している。１５は積
層電極カートリツジホルダーである。 FIG. 1 is a longitudinal sectional view showing an embodiment of a laminated electrode cartridge according to the present invention. The calomel reference electrode electrolyte injection hole 1 is connected to the reference electrode electrolyte side of the main body of the measuring device, and each electrode that makes up the whole consists of blocks arranged adjacent to each other that can be converted into each other. , calomel reference electrode 2, chlorine electrode 3, calcium electrode 4, potassium electrode 5,
An enzyme membrane electrode 6 for oxygen or hydrogen peroxide, a sodium electrode 7, etc. are arranged, and they are connected to terminals 8 and 9.
The calomel reference electrode is connected to the potentiometer or ammeter of the measuring device through the porous plate 10.
An enzyme immobilization membrane 11 is provided on the enzyme membrane electrode, and one of the sample holes 12 communicates with a blood collection needle connection part 13 and the other with a blood collection syringe connection part 14. 15 is a laminated electrode cartridge holder.
また、第２図により本発明装置の横断面を説明
すれば、試料通路１６を中心に、その外側に隔膜
固定リング１７、そのまた外側に電解液室１８、
最後にホルダー本体部分１９が連なつており、電
解液室から外側部分に向けて電極ターミナル及び
リード線２０が接続されている。 Further, to explain the cross section of the device of the present invention with reference to FIG. 2, centering on the sample passage 16, there is a diaphragm fixing ring 17 on the outside, an electrolyte chamber 18 on the outside,
Finally, a holder main body portion 19 is connected, and electrode terminals and lead wires 20 are connected from the electrolyte chamber to the outer portion.
このような構成からなる本発明の装置の操作に
ついて、第３図により説明する。 The operation of the apparatus of the present invention having such a configuration will be explained with reference to FIG.
積層電極カートリツジ（以下、カートリツジと
略）本体２１に採血針と注射器を接続して、患者
の静脈より血液を必要量採血した後、直ちに、水
平に維持したまま採血針２２と注射器２３をカー
トリツジから分離し、直ちにカートリツジを計測
機本体２４に差し込む。 After connecting the blood collection needle and syringe to the laminated electrode cartridge (hereinafter referred to as cartridge) main body 21 and collecting the required amount of blood from the patient's vein, immediately remove the blood collection needle 22 and syringe 23 from the cartridge while keeping it horizontal. After separating, immediately insert the cartridge into the measuring machine main body 24.
（ここまでは、採血から30秒以内に行うことが望
しい。）この操作によつて、始動スイツチＳが押
され、その後は機内の自動制御により、まず、参
照電極用電解液（塩化カリウム又は硫酸カリウム
の溶液を用いる。）が約3μほど主流路に向つて
排水され、これにより試料と参照電極２の間の接
触を確実にしたうえで、各電極の電位差を読み取
つて記憶させ、次いで標準液Ｂを、カートリツジ
内の血液を押し出し、かつ洗い流すように注入さ
せ、再び各電極に生じた電位をとらえ、先の試料
によつて得られた電位と比較して試料のイオン濃
度を演算部で算出し、表示部へ表示する。図４〜
９の検量線は、以上の操作によつて得たものであ
る。(It is desirable to perform this step within 30 seconds after blood collection.) With this operation, the start switch S is pressed, and after that, the automatic control inside the machine will first start with the reference electrode electrolyte (potassium chloride or (using a solution of potassium sulfate) is drained toward the main channel by approximately 3μ, thereby ensuring contact between the sample and reference electrode 2, the potential difference of each electrode is read and memorized, and then the standard Liquid B is injected to push out and wash away the blood in the cartridge, and the potential generated at each electrode is captured again. The ion concentration of the sample is calculated by the calculation unit by comparing it with the potential obtained with the previous sample. Calculate and display on the display. Figure 4~
The calibration curve No. 9 was obtained by the above procedure.
計測機本体内の標準液Ａ，ＣはＢと共に毎日の
測定開始時にカートリツジ内を順次に通過させ
て、電位差とイオン濃度の関係を演算部に記憶さ
せる。（これにより、電極の較正又は標準化を行
う。）
本発明によれば、緊急時又は一般の診療におい
て採血と同時に、血漿を分離することなく、血漿
中成分の定量分析が行えるもので、従つて、本発
明のカートリツジに組み込んでない、他の生体成
分の測定については、注射器内２３に採取されて
いる血液を所定の採血容器に移して、血清を分離
して測定検査することができる。もし、検査する
目的生体成分の測定項目が全てカートリツジに組
み込まれている場合には、血液がカートリツジ内
を満たしたならば、それ以上の採血は不要であ
る。 Standard solutions A and C in the measuring instrument main body are sequentially passed through the cartridge together with B at the start of daily measurement, and the relationship between potential difference and ion concentration is stored in the calculation unit. (This calibrates or standardizes the electrodes.) According to the present invention, quantitative analysis of plasma components can be performed at the same time as blood collection in emergencies or general medical practice without separating plasma. For the measurement of other biological components not incorporated in the cartridge of the present invention, the blood collected in the syringe 23 can be transferred to a predetermined blood collection container, and the serum can be separated for measurement and testing. If all the measurement items for the target biological component to be tested are included in the cartridge, once the cartridge is filled with blood, there is no need to collect any more blood.
もちろん、本発明の積層電極カートリツジの各
電極は連続して隣接して配設された独立したブロ
ツクでそれぞれ個々に変換が可能であるように設
計されているため、例えば酸素又は過酸化水素用
酵素膜電極での酵素固定化膜には、測定する検査
項目に応じて、グルコースオキシダーゼ、ウリカ
ーゼ、カタラーゼ、ピルビン酸オキシダーゼ、Ｌ
−アミノ酸オキシダーゼ等に代えられ、イオン電
極にはPH、NH3、尿素、Li、Cu2+、pb2+、Ag+、
F-等公知のものに代えて利用することができる。 Of course, each electrode of the laminated electrode cartridge of the present invention is designed to be able to be converted individually in successive and adjacent independent blocks, such as enzymes for oxygen or hydrogen peroxide. The enzyme-immobilized membrane at the membrane electrode contains glucose oxidase, uricase, catalase, pyruvate oxidase, L, depending on the test item to be measured.
- Amino acid oxidase, etc., and the ionic electrode contains PH, NH 3 , urea, Li, Cu 2+ , pb 2+ , Ag + ,
It can be used in place of known ones such as F- .
以上説明したように本発明によれば、積層電極
カートリツジ方式の小形で簡便な装置を採血用の
注射針と注射器の間にセツトして患者から検体
（血液）を採取する際に、その過程で本発明の装
置に盛込まれた積層電極により、臨床的情報を検
体から取り出すことができ、かつ注射器に採取し
た検体は、そのまま他の部分の情報を得るための
試料として用いることができ、緊急検査における
迅速、正確、簡便という要求に答えることができ
る。 As explained above, according to the present invention, when a sample (blood) is collected from a patient by setting a small and simple device using a laminated electrode cartridge between a blood collection needle and a syringe, The laminated electrodes incorporated into the device of the present invention allow clinical information to be extracted from the specimen, and the specimen collected into the syringe can be used as it is as a sample to obtain information on other parts, making it possible to extract clinical information from the specimen. It can meet the demands for rapid, accurate, and simple testing.
第１図は本発明装置の一実施例を説明するため
の縦断面図、第２図は本発明装置の一実施例を説
明するための横断面の図、第３図は本発明装置の
一実施例を説明するための全体の概略構成図、第
４〜９図は本発明装置によつて測定された生体成
分の濃度に対する電位を示す検量線である。
１……参照電極用電解液注入孔、２……参照電
極、３……塩素電極、４……Caの電極、５……
Ｋ電極、６……酵素膜電極、７……Naの電極、
８，９……各電極のターミナル、１０……多孔質
板、１１……固定化酵素膜、１２，１６……試料
通路、１３……採血針接続部分、１４……採血注
射器接続部分、１５，１９……ホルダー、１７…
…隔膜固定リング、１８……電解液室、２０……
リード線、２１……積層電極カートリツジ本体、
２２……採血針、２３……注射容器、２４……計
測機本体。
FIG. 1 is a longitudinal cross-sectional view for explaining an embodiment of the apparatus of the present invention, FIG. 2 is a cross-sectional view for explaining an embodiment of the apparatus of the present invention, and FIG. 3 is a cross-sectional view of the apparatus of the present invention. FIGS. 4 to 9, which are overall schematic configuration diagrams for explaining the embodiment, are calibration curves showing potentials versus concentrations of biological components measured by the apparatus of the present invention. 1... Reference electrode electrolyte injection hole, 2... Reference electrode, 3... Chlorine electrode, 4... Ca electrode, 5...
K electrode, 6... Enzyme membrane electrode, 7... Na electrode,
8, 9... Terminal of each electrode, 10... Porous plate, 11... Immobilized enzyme membrane, 12, 16... Sample passage, 13... Blood collection needle connection part, 14... Blood collection syringe connection part, 15 , 19...Holder, 17...
...Diaphragm fixing ring, 18... Electrolyte chamber, 20...
Lead wire, 21...Laminated electrode cartridge body,
22...Blood collection needle, 23...Injection container, 24...Measuring device main body.
Claims (1)
れ個々に変換可能であるブロツクが連続して隣接
して配設された積層電極カートリツジで、これを
採血針と採血用注射器の間に設置し、一回の試料
注入によつて多項目の生体成分を同時に測定する
ことを特徴とする積層電極カートリツジを用いた
多項目同時測定装置。1 Each electrode consists of an independent block, and each block can be converted individually. It is a laminated electrode cartridge in which the blocks are arranged adjacent to each other in succession. This is installed between a blood collection needle and a blood collection syringe, and one time A multi-item simultaneous measurement device using a laminated electrode cartridge, which is characterized by simultaneously measuring multiple biological components by injecting a sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58072247A JPS59200633A (en) | 1983-04-26 | 1983-04-26 | Multi-term simultaneous measuring apparatus using laminated elctrode cartridge |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58072247A JPS59200633A (en) | 1983-04-26 | 1983-04-26 | Multi-term simultaneous measuring apparatus using laminated elctrode cartridge |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS59200633A JPS59200633A (en) | 1984-11-14 |
| JPH0318455B2 true JPH0318455B2 (en) | 1991-03-12 |
Family
ID=13483769
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP58072247A Granted JPS59200633A (en) | 1983-04-26 | 1983-04-26 | Multi-term simultaneous measuring apparatus using laminated elctrode cartridge |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS59200633A (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6411532A (en) * | 1987-07-03 | 1989-01-17 | Terumo Corp | Continuous monitoring instrument |
-
1983
- 1983-04-26 JP JP58072247A patent/JPS59200633A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS59200633A (en) | 1984-11-14 |
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