JPS56151357A - Immunoassay method - Google Patents
Immunoassay methodInfo
- Publication number
- JPS56151357A JPS56151357A JP5415780A JP5415780A JPS56151357A JP S56151357 A JPS56151357 A JP S56151357A JP 5415780 A JP5415780 A JP 5415780A JP 5415780 A JP5415780 A JP 5415780A JP S56151357 A JPS56151357 A JP S56151357A
- Authority
- JP
- Japan
- Prior art keywords
- substrate
- measured
- antigens
- fine particles
- tested
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000003018 immunoassay Methods 0.000 title 1
- 239000000758 substrate Substances 0.000 abstract 7
- 239000010419 fine particle Substances 0.000 abstract 6
- 239000000427 antigen Substances 0.000 abstract 5
- 102000036639 antigens Human genes 0.000 abstract 5
- 108091007433 antigens Proteins 0.000 abstract 5
- 239000007788 liquid Substances 0.000 abstract 4
- 238000005259 measurement Methods 0.000 abstract 2
- 239000000725 suspension Substances 0.000 abstract 2
- 238000010521 absorption reaction Methods 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
Abstract
PURPOSE:To enable a simultaneous measurement of a large number of items and to permit automation of said measurement, by a method wherein antibodies against an object to be measured are secured on a substrate. CONSTITUTION:A substrate 1, which has atinbodies 2 against an object to be measured, secured on the surface thereof, is prepared, and a liquid to be tested, containing antigens 3 being the object to be measured, is brought into contact with the substrate for a specific period of time. This causes absorption of antigens in the liquid to be tested to the substrate through the working of the antibodies. After the substrate is well washed, it is brought into contact with a suspension which is prepared separately from the above and suspends fine particles 4 having antibodies 5 against the object to be measured on the surface of the fine particle, and this causes reaction of the antigens, absorbed on the substrate, to the antibodies on the surfaces of the fine particles. After a given period of time has elapsed, unreacted suspension fine particles are washed out of the substrate to prepare a specimen on which the number of the fine particles, corresponding to the amount and the density of the antigens in the liquid to be tested, are secured. The number of the fine particles on the specimen are measured, and this finds the amount and density of the antigens in the liquid to be tested.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5415780A JPS56151357A (en) | 1980-04-25 | 1980-04-25 | Immunoassay method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP5415780A JPS56151357A (en) | 1980-04-25 | 1980-04-25 | Immunoassay method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS56151357A true JPS56151357A (en) | 1981-11-24 |
Family
ID=12962704
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5415780A Pending JPS56151357A (en) | 1980-04-25 | 1980-04-25 | Immunoassay method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS56151357A (en) |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5814057A (en) * | 1981-07-17 | 1983-01-26 | Toray Ind Inc | Measurement of biologically active substances and labelling agent used therefor |
| JPS5860256A (en) * | 1981-10-06 | 1983-04-09 | Toray Ind Inc | Measurement of biologically active substance and label agent used therefor |
| JPS5975152A (en) * | 1982-10-23 | 1984-04-27 | Tatsuo Suzuta | Fluorescent immunity latex particle |
| JPS59122950A (en) * | 1982-12-28 | 1984-07-16 | Toray Ind Inc | Method for measuring biologically active substance and labeling agent used therein |
| JPS60253870A (en) * | 1984-05-08 | 1985-12-14 | Sekisui Chem Co Ltd | Immunological diagnosis |
| JPS6148764A (en) * | 1984-08-14 | 1986-03-10 | デューク サイエンティフィック コーポレーション | Method and kit for testing aqueous sample for specific substance |
| WO1986007463A1 (en) * | 1985-06-03 | 1986-12-18 | American National Red Cross | Microtiter - surface - flocculation assay for antigen or antibody screening |
| JPS628056A (en) * | 1985-07-05 | 1987-01-16 | Yoichi Shibata | Specific bonding analysis |
| US4663277A (en) * | 1983-05-20 | 1987-05-05 | Profile Diagnostic Sciences Inc. | Virus detection method and materials |
| US5061237A (en) * | 1985-07-02 | 1991-10-29 | Cytomed Medizintechnik Gmbh | Method of purifying whole blood |
| WO1992006379A1 (en) * | 1990-10-09 | 1992-04-16 | Idemitsu Petrochemical Co., Ltd. | Method of immunological quantitative analysis |
| US5308990A (en) * | 1991-05-15 | 1994-05-03 | Hitachi, Ltd. | Method for measuring microparticles, quantitative measuring method therefor and instrument for measuring microparticles |
| JP2007262525A (en) * | 2006-03-29 | 2007-10-11 | Jfe Steel Kk | Dust removal method and apparatus for converter exhaust gas treatment facility |
-
1980
- 1980-04-25 JP JP5415780A patent/JPS56151357A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5814057A (en) * | 1981-07-17 | 1983-01-26 | Toray Ind Inc | Measurement of biologically active substances and labelling agent used therefor |
| JPS5860256A (en) * | 1981-10-06 | 1983-04-09 | Toray Ind Inc | Measurement of biologically active substance and label agent used therefor |
| JPS5975152A (en) * | 1982-10-23 | 1984-04-27 | Tatsuo Suzuta | Fluorescent immunity latex particle |
| JPS59122950A (en) * | 1982-12-28 | 1984-07-16 | Toray Ind Inc | Method for measuring biologically active substance and labeling agent used therein |
| US4663277A (en) * | 1983-05-20 | 1987-05-05 | Profile Diagnostic Sciences Inc. | Virus detection method and materials |
| JPS60253870A (en) * | 1984-05-08 | 1985-12-14 | Sekisui Chem Co Ltd | Immunological diagnosis |
| JPS6148764A (en) * | 1984-08-14 | 1986-03-10 | デューク サイエンティフィック コーポレーション | Method and kit for testing aqueous sample for specific substance |
| WO1986007463A1 (en) * | 1985-06-03 | 1986-12-18 | American National Red Cross | Microtiter - surface - flocculation assay for antigen or antibody screening |
| US5061237A (en) * | 1985-07-02 | 1991-10-29 | Cytomed Medizintechnik Gmbh | Method of purifying whole blood |
| JPS628056A (en) * | 1985-07-05 | 1987-01-16 | Yoichi Shibata | Specific bonding analysis |
| WO1992006379A1 (en) * | 1990-10-09 | 1992-04-16 | Idemitsu Petrochemical Co., Ltd. | Method of immunological quantitative analysis |
| US5308990A (en) * | 1991-05-15 | 1994-05-03 | Hitachi, Ltd. | Method for measuring microparticles, quantitative measuring method therefor and instrument for measuring microparticles |
| JP2007262525A (en) * | 2006-03-29 | 2007-10-11 | Jfe Steel Kk | Dust removal method and apparatus for converter exhaust gas treatment facility |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JPS56151357A (en) | Immunoassay method | |
| ES8200770A1 (en) | Latex reagent, process for its preparation, its application and an agent containing this reagent. | |
| JPS6432169A (en) | Immunochromatograph method using colloidal particle | |
| IL80655A0 (en) | Process for the quantification of cell populations or subpopulations and reagent suitable therefor | |
| EP0163312A3 (en) | Immunoassay | |
| ATE786T1 (en) | METHODS FOR DETECTING AND/OR DETERMINING THE COMPONENTS OF THE REACTION BETWEEN A SPECIFIC BINDING PROTEIN AND THE MATCHING SUBSTANCE TO BE BINDED, IN AN AQUEOUS SAMPLE; FREEZE-DRIED REAGENT AND REAGENT PACK FOR USE IN THIS PROCEDURE. | |
| DE3264913D1 (en) | Method of carrying out an analytical determination, and suitable means therefor | |
| DK164690A (en) | Process for the manufacture of vegetable-fibre based boards, in particular particle boards or fibre boards | |
| JPS6447953A (en) | Method and apparatus for separating labelled reagent for testing immunological bond measuring test | |
| JPS55162059A (en) | Measuring method for antigen, antibody or their complex and measurement reagent kit | |
| ES480964A1 (en) | Process for detecting and quantifying an antigen or antibody. | |
| ES467831A1 (en) | Enzyme modifier immunoassay | |
| CA2023803A1 (en) | Method for measuring an immunologically active material and apparatus suitable for practicing said method | |
| GB2005019A (en) | Magnetically attractable material for use in immunoassay | |
| ES8706210A1 (en) | Device and method for analysis of biological material | |
| EP0157197A3 (en) | Method for carrying out immunofluorescent assays | |
| EP0304794A3 (en) | Quantitative measurement of lithium | |
| ATE138203T1 (en) | TESTING METHODS FOR ANTIGENS OR ANTIBODIES | |
| DE3070851D1 (en) | Ria for hepatitis a antigen and antigen antibody radionuclide therefor | |
| JPS55146044A (en) | Discriminating method for blood type | |
| JPS5570742A (en) | Measuring method of engyme immunity of hito placenta lactogen with sandwich method and kit used for it | |
| JPS5739354A (en) | Control method for automatic analytical apparatus of biochemistry | |
| JPS56125667A (en) | Reagent for measurement and measuring method for human muscle type aldolase | |
| JPS5658637A (en) | Measuring method for diameter of particle | |
| JPS5629168A (en) | Measuring method of body fluid component by immunity chemical reaction |