JPS59182347A - Automatic analytical instrument - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION (a) Industrial application field This invention is directed to the optical characteristics of a reaction solution produced by adding an analytical reagent to each of a group of samples/sample solutions and reacting them under predetermined conditions. is an automatic analyzer that measures the concentration of an analyte component based on absorbance of visible light, ultraviolet, near-infrared, etc., fluorescence intensity, light scattering intensity of a specific wavelength, etc., and the analytical reagent has a predetermined titer. The present invention relates to an automatic analyzer configured to perform analysis while automatically confirming that a reaction has completed normally.

(B) Prior Art Conventionally, automatic analyzers measure the concentration of the analyte component from the optical properties of the reaction liquid produced by adding analytical reagents to a group of sample liquids and reacting them under predetermined conditions. It is common to measure and analyze the optical properties of each sample after the time required to complete the process. Furthermore, some of these analyzers are equipped with an analytical reagent cooling device in order to store a considerable amount of analytical reagent prepared at one time and prevent the titer from deteriorating. After all the stored analytical reagents are used up, newly prepared analytical reagents are filled. In order to efficiently analyze a large number of samples with an automatic analyzer, the analytical reagents once filled may not be checked until they are finished being used. If an endpoint analysis method (for example, an enzyme reaction analysis of glucose in serum) is performed with reagents that have deteriorated, reproducibility will decrease due to poor sensitivity, and if the reagent deteriorates significantly, the measured value will deviate from the true value. becomes. Furthermore, when determining the device constant (K value) in a late assay method (for example, an assay for the enzyme activity of GPT), if the analytical reagent has deteriorated, the value will be a value that is close to the true value.

(c) Purpose of the Invention This invention was made to solve the above-mentioned problems. The purpose of this invention is to provide an automatic analyzer that can perform analysis while automatically performing one test.

B) Structure of the Invention This invention is a method for determining the concentration of an analyte component from the optical properties of a reaction solution produced by adding a predetermined analytical reagent to a large number of sample liquids containing the analyte component and reacting them under predetermined conditions. This is an automatic analyzer that continuously or intermittently performs quantitative determination, and the optical properties of the reaction solution and reagent blank solution are determined at an arbitrarily determined time (tt, t2, .
..., tn-1, 1n), and the measured values of the reaction solution at t, t2, ..., tn-1, tn, At □, At 2, . ...,
At, n-0, Atn: Measured values of F6 and reagent blank solution AR engineering, AR2, ... Zeng, AR unit, AR-stuck, formula: Calculate the numerical value expressed by % formula %, and each It is determined whether the numerical values are above a predetermined threshold value, and if each numerical value is above the threshold value, the Atn value is converted into the concentration of the analyte component and displayed, and -1 is calculated for each calculated value. If the titer is below the threshold, it is equipped with a means to display or notify that the analysis is abnormal. The present invention provides an automatic analyzer characterized in that it is configured so that analysis can be performed while confirming the accuracy of the analysis.

(e) Examples The present invention will be described below based on examples shown in the drawings. However, this invention is not limited to this.

FIG. 1 is an explanatory diagram of the configuration of an embodiment of an automatic analyzer of the present invention, which measures the concentration of an analyte component by measuring the absorbance of a reaction solution.

First, the same amount of pure water or physiological saline (1) as the liquid sample used for the second analysis is collected with the pipetter (2) and into the container (4) of the reaction/measuring container line (3). It is sequentially driven and moved in the direction of arrow (a). Next, a predetermined amount of analytical reagent (
5) with the pipettor (6) and inject it as a reagent blank solution. - A sample collection pipettor (9) separates a predetermined amount of No. 1 sample liquid from one container of a group of homogeneous samples arranged on a sample installation table (8) in a universal container (7),
It is sequentially driven and moved in the direction of arrow [a]. Next, a predetermined amount of the analytical reagent (5) collected by the analytical reagent pipetter (6) is injected into the container (7) to start a reaction.
The generated No. 1 sample reaction solution is sequentially driven and moved in the direction of arrow (a). Next, a predetermined amount of another No. 2 sample solution No. 0 is sequentially collected in the subsequent reaction/measuring container in the same manner as above, and the analytical reagent (5) is added to start the reaction. A reaction solution for sample m is prepared and sequentially moved in the direction of arrow (a).

The reagent blank solution is moved sequentially for a predetermined time (t) after the preparation of the reagent blank solution.
Step) When the container of the reagent blank solution that has passed has reached the position (A), the light of 10i is transmitted through the reagent blank solution through a light source lamp (manufactured by Optical Fiber Co., Ltd.). Only light passes through the detector (
Enter 131. Furthermore, after a predetermined time (t2) has elapsed since the reagent blank solution was prepared, (B)
When reaching the position, light source lamp 00) → reagent blank liquid at optical fiber 141-B position →
The transmitted light enters the detector section along the path from filter 115) to detector (16). Furthermore, this reagent blank liquid container is changed to (0) after a predetermined time (t3) has elapsed since the reagent blank container was prepared.
When the position is reached, the light source lamp (10) →
Optical fiber 〇'71-reagent flank liquid-filter (decoy-detector (19) path through which the impinging light enters the detector u9.

The transmitted light signal from each detector [131, TIED, (19) enters a multiplexer (20+), selects a standard specific wavelength, and then passes through an absorbance converter Cυ to an absorbance memory part. , after the elapse of time t22 and t3, the absorbance of the reagent blank solution in 2
8 and AIR3 are stored.

When the reaction solution of sample No. 1 is sequentially moved and reaches the position [A] after a predetermined time (tρ has elapsed after the start of the reaction), the light source lamp (10-optical fiber
11) - No. 1 sample reaction solution at position A - Filter (12
- detector (transmitted light power S detector with 13 paths t131 l
Come in. Next, at a predetermined time tLl'l' (t
2) When reaching the position (B) after the elapse of time, the light source lamp αD → optical fiber) < - (141-After reaction of the first sample at the B position - filter (15) - detector (
It enters the transmitted force S detector ae through the path 161. Further, when the predetermined time (1) after the start of the reaction has passed and the position (C) is reached, the light source lamp (il1 → optical fiber (lη → negative No. 31 sample reaction liquid at position C) - filter (1
8) The transmitted light enters the detector a wing on the path of →detector 091. And each detector σ31. The transmitted light signal from (16), t19) is sent to a multiplexer (20+4, a standard specific wavelength is selected, and then) to an absorbance converter (2
1) The absorbance signal is converted into an absorbance signal and entered into a part of the upper memory. After the reaction of the first sample reaction solution has started, each absorbance after 11 hours has elapsed) ￥, degree Alt, IS
2 3 At and Alt3 are stored.

2 Next, a part of the absorbance memory is read, and each of the absorbance values is calculated by the absorbance determination section.

l A: tt knee AIR engineering l) V, (I) And first, the calculated value on the left side of equation (1) is greater than a predetermined threshold value stored in advance. If it is less than 26j, the abnormality will be displayed on the display section (26j receives the abnormal signal power i and displays %). Also, the above continues (
n) It is determined whether the calculated number fK on the left side of the equation is greater than or equal to a predetermined threshold value 73 stored in advance. If it is less than or equal to 2, abnormal force 5 is displayed in the same manner as above. Also, if it is v2 or higher, it is determined whether the calculated value on the left side of the equation is equal to or higher than the pre-stored threshold value 73, and if it is lower than v3, an abnormality is displayed as above. Ru. Furthermore, when an abnormality is displayed (the analysis is automatically stopped and /'f or measured value S is printed. Furthermore, if it is 73 or more, the signal of absorbance Alt3 is displayed on the concentration converter). The analysis result of the concentration of the component to be analyzed is displayed on display 8 and/or printed.

Samples No. 2 to No. m are also processed in the same procedure as the No. 1 sample, and when an abnormality is displayed, the analysis is automatically stopped or the window and/or the measured value is printed.

Note that the above series of operations are performed on the control section.

The absorbance of the reagent blank solution only needs to be measured once at the beginning of continuous measurement of a group of sample solutions as described above.

As for the t-process, if the titer of the analytical reagent is normal, the time is selected when the reaction used for analysis is about 40 to 60% complete. The time at which the reaction rate is 70 to 90% complete is selected as t2, and the time at which the reaction is sufficiently completed is selected as t3.

Further, the threshold values V, (2) and (3) are appropriately selected so that it can be checked whether the reaction is progressing normally based on the above-mentioned times t-t3.

FIG. 2 shows reagent blank solution preparation or reaction start time t. This is a graph showing the absorbance of the reagent blank solution and the No. 1 sample reaction solution after t to t3, and curves X and x2 are graphs when the titer of the analytical reagent has deteriorated.

What? The number of times the reagent blank solution or sample reaction solution is measured over time depends on the type of reaction used for the analysis, but 3 to 4 times is generally sufficient.

A specific example of analysis using the above-mentioned absorbance is the analysis of glucose in serum or plasma, in which a quinone dye produced based on the following reaction formula is measured using the absorbance of light at a wavelength of 510 nm.

2 H202 + Phenol + 4-aminoantipyrine peroxidase □ Quinone dye + 4H20 As another specific example, when analyzing the enzymatic activity of GPT based on the following reaction, NADH based on reaction formula (B)
The device constant (K
An example of this is when measuring a value).

GPT L-alanine + α-ketoglutaric acid -> Glutamic acid +
Pyruvate (A) DH Pyruvate 10NADH->Lactic acid 10NA D
(B) In this case, since the deterioration of the analytical reagent has a large effect on the measured value, it is particularly advantageous to measure using this automatic analyzer.

The above example is based on absorbance measurement, but when using the fluorescence intensity or light scattering intensity of the reaction solution as an optical property other than this, the optical property measurement block ring surrounded by the dotted line in Fig. 1 uses fluorescence. The system is converted into a system for measuring intensity and light scattering intensity, and other systems are appropriately modified accordingly.

(F) Effects of the Invention According to the automatic analyzer of the present invention, it is possible to continuously analyze a large number of samples while automatically checking for deterioration of analytical reagents and confirming that the reaction has completed normally. .

Therefore, it is possible to avoid analyzes that give measured values that deviate from the true values (measured values with poor visibility and force) due to deterioration of reagents.

[Brief explanation of drawings]

FIG. 1 is an explanatory diagram of the configuration of one embodiment of the automatic analyzer of the present invention, and FIG. 2 is a graph showing the relationship between absorbance and time when measured by the end point method using the apparatus shown in FIG. (1)...-Pure water or physiological saline, (2i (6
1 (9i... pipettor, (5)... analytical reagent,
(3)...Reaction and measurement vessel line, +41 +71...Reaction and measurement vessel, (8j-sample installation table, tlol...light source lamp, (Ill (141G7)...optical fiber, [121 (151) (181...filter, (1
3i u61 (191・Detector, (20)...Multiplexer, 211...Absorbance converter, @...Absorbance memory part, □□□...Absorbance determination section, (2)・
...concentration converter, □□□)...display section, and)...control section.

Claims (1)

[Claims] 1. Continuous determination of the concentration of the analyte component from the optical properties of the reaction solution produced by adding a predetermined analytical reagent to a large number of sample solutions containing the analyte component and reacting them under predetermined conditions. An automatic analyzer that quantitatively or intermittently determines the optical properties of the reaction solution 8 and the reagent blank solution at an arbitrarily determined time (t, t2, . . . after adding the analytical reagent).
. . . , t n-1, t 2,...
, Atn-□, Atn2 and reagent blank solution measurement values A
Calculate the numerical values expressed by the formula: % formula % for R, AR2, ..., AR, n-0, ARn, and determine whether each numerical value is greater than a predetermined threshold. If each value is above the threshold value, the At value is converted to the concentration of the acid to be analyzed and displayed, and if any of the calculated aromatic values is below the threshold value, the analysis is abnormal. The method is characterized in that it is equipped with a means for displaying or notifying the sample, and is configured so that each sample can be analyzed without automatically confirming that the analytical reagent has a predetermined titer and that the reaction has completed normally. automatic analyzer.