JPS6429768A - Method and apparatus for laser magnetic immunoassay - Google Patents
Method and apparatus for laser magnetic immunoassayInfo
- Publication number
- JPS6429768A JPS6429768A JP18490287A JP18490287A JPS6429768A JP S6429768 A JPS6429768 A JP S6429768A JP 18490287 A JP18490287 A JP 18490287A JP 18490287 A JP18490287 A JP 18490287A JP S6429768 A JPS6429768 A JP S6429768A
- Authority
- JP
- Japan
- Prior art keywords
- specimen
- reflected light
- magnetic
- magnetic material
- electromagnet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000003018 immunoassay Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 239000000696 magnetic material Substances 0.000 abstract 3
- 230000001360 synchronised effect Effects 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- 239000000427 antigen Substances 0.000 abstract 1
- 102000036639 antigens Human genes 0.000 abstract 1
- 108091007433 antigens Proteins 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 230000005279 excitation period Effects 0.000 abstract 1
- 230000004907 flux Effects 0.000 abstract 1
- 238000002372 labelling Methods 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 230000004899 motility Effects 0.000 abstract 1
Landscapes
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
PURPOSE:To quantitatively detect a specific antibody or antigen from a slight amt. of a magnetic material-labeled specimen by controlling the motility of the specimen by magnetic force and the surface tension of the liquid and detecting the reflected laser light or interference fringes synchronized with the control. CONSTITUTION:A soln. contg., for example, the magnetic material-labeled specimen 20 (complex of the magnetic material labeling body and the specimen) after, for example, an antigen- antibody reaction is housed in an inspecting vessel 1. The magnetic fluxes generated from an electromagnet 3 focus to a magnetic pole piece 5 placed right above the vessel 1 and, therefore, the specimen 20 is concd. on the water surface right under the magnetic pole piece 5 and is strongly attracted to the piece 5, by which the water surface around the same is built up. The interference fringes corresponding to the degree of the build-up are generated in the reflected light 8 when the laser light 7 is projected on this very small build-up part 21. The quantity of the specimen 20 is known from the number of the fringes. The reflected light 8 synchronizes with excitation periods and a change in the intensity of the reflected light is caused if the electromagnet 31 is alternately excited. The influence of external disturbance and background is effectively removed if only the fluctuation component synchronized with the alternating frequencies is detected in the detection of the reflected light.
Priority Applications (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62184902A JPH0820450B2 (en) | 1987-07-24 | 1987-07-24 | Laser magnetic immunoassay method and apparatus |
| US07/221,248 US5252493A (en) | 1986-09-22 | 1987-09-22 | Laser magnetic immunoassay method and apparatus therefor |
| EP87906109A EP0287665B1 (en) | 1986-09-22 | 1987-09-22 | Laser magnetic immunoassay method and apparatus therefor |
| DE3751865T DE3751865T2 (en) | 1986-09-22 | 1987-09-22 | LASER MAGNETIC IMMUNITY TEST METHOD AND DEVICE THEREFOR |
| PCT/JP1987/000694 WO1988002118A1 (en) | 1986-09-22 | 1987-09-22 | Laser magnetic immunoassay method and apparatus therefor |
| US07/915,022 US5238810A (en) | 1986-09-22 | 1992-07-15 | Laser magnetic immunoassay method and apparatus thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62184902A JPH0820450B2 (en) | 1987-07-24 | 1987-07-24 | Laser magnetic immunoassay method and apparatus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6429768A true JPS6429768A (en) | 1989-01-31 |
| JPH0820450B2 JPH0820450B2 (en) | 1996-03-04 |
Family
ID=16161326
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62184902A Expired - Lifetime JPH0820450B2 (en) | 1986-09-22 | 1987-07-24 | Laser magnetic immunoassay method and apparatus |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0820450B2 (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02280057A (en) * | 1989-04-20 | 1990-11-16 | Nippon Telegr & Teleph Corp <Ntt> | Method and apparatus for non-separation type laser magnetic immunoassay |
| US5236824A (en) * | 1988-04-26 | 1993-08-17 | Nippon Telegraph And Telephone Corporation | Laser magnetic immunoassay method and method by a magnetophoresis apparatus therefor |
| US5238811A (en) * | 1988-04-26 | 1993-08-24 | Nippon Telegraph And Telephone Corporation | Laser magnetic immunoassay method and apparatus therefor and superparamagnetic material-labeled body and method for the manufacture of same |
| US5340749A (en) * | 1988-04-26 | 1994-08-23 | Nippon Telegraph And Telephone Corporation | Method for collecting and preparing specimens for immune reactions |
| US6379310B1 (en) | 1998-01-13 | 2002-04-30 | Omron Corporation | Wrist sphygmomanometer |
| US6893402B2 (en) | 1997-12-24 | 2005-05-17 | Braun Gmbh | Method and measuring device for determining blood pressure |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62118255A (en) * | 1985-11-19 | 1987-05-29 | Toshimitsu Musha | Detection of immunological reaction by using magnetic field |
-
1987
- 1987-07-24 JP JP62184902A patent/JPH0820450B2/en not_active Expired - Lifetime
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62118255A (en) * | 1985-11-19 | 1987-05-29 | Toshimitsu Musha | Detection of immunological reaction by using magnetic field |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5236824A (en) * | 1988-04-26 | 1993-08-17 | Nippon Telegraph And Telephone Corporation | Laser magnetic immunoassay method and method by a magnetophoresis apparatus therefor |
| US5238811A (en) * | 1988-04-26 | 1993-08-24 | Nippon Telegraph And Telephone Corporation | Laser magnetic immunoassay method and apparatus therefor and superparamagnetic material-labeled body and method for the manufacture of same |
| US5340749A (en) * | 1988-04-26 | 1994-08-23 | Nippon Telegraph And Telephone Corporation | Method for collecting and preparing specimens for immune reactions |
| US5498550A (en) * | 1988-04-26 | 1996-03-12 | Nippon Telegraph & Telephone Corporation | Device for collecting or preparing specimens using magnetic micro-particles |
| JPH02280057A (en) * | 1989-04-20 | 1990-11-16 | Nippon Telegr & Teleph Corp <Ntt> | Method and apparatus for non-separation type laser magnetic immunoassay |
| US6893402B2 (en) | 1997-12-24 | 2005-05-17 | Braun Gmbh | Method and measuring device for determining blood pressure |
| US7963921B1 (en) | 1997-12-24 | 2011-06-21 | Kaz Usa, Inc. | Method and measuring device for determining blood pressure |
| US6379310B1 (en) | 1998-01-13 | 2002-04-30 | Omron Corporation | Wrist sphygmomanometer |
| US6547741B2 (en) | 1998-01-13 | 2003-04-15 | Omron Corporation | Wrist sphygmomanometer |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0820450B2 (en) | 1996-03-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5238815A (en) | Enzymatic immunoassay involving detecting fluorescence while oscillating magnetic beads | |
| EP0262760B1 (en) | Method and apparatus for immunoassay | |
| EP0216177B1 (en) | Enzymatic immunoassay | |
| ATE67858T1 (en) | IMMUNOLOGICAL TEST PROCEDURE. | |
| ATE57577T1 (en) | IMMUNOASSAY METHODS USING PATTERNS FOR DETECTING SOLUBLE ANTIGENS AND CELL SURFACE ANTIGENS. | |
| JPS6429768A (en) | Method and apparatus for laser magnetic immunoassay | |
| MY102881A (en) | Method of automatically detecting agglutination reaction and apparatus therefor | |
| JP5356219B2 (en) | Chemical detector | |
| DE68922126D1 (en) | Immunometric method for the detection of lipoprotein particles, anti-Lp (a) monoclonal antibodies and their use for the diagnosis of atherosclerosis. | |
| ATE138203T1 (en) | TESTING METHODS FOR ANTIGENS OR ANTIBODIES | |
| JPH07111430B2 (en) | Laser magnetic immunoassay method and measuring apparatus | |
| EP0369176A3 (en) | Method for immunoassay using photoacoustic spectroscopy | |
| JPS579723A (en) | Stabilizing agent for immunological reaction and measuring method of antigen-antibody reaction | |
| JPS63188766A (en) | Method and instrument for laser magnetic immunoassay | |
| JPH07111429B2 (en) | Laser magnetic immunoassay | |
| JP2509227B2 (en) | Laser magnetic immunoassay device | |
| ATE26346T1 (en) | PROCEDURE FOR DETECTING ANTIGENS AND ANTIBODIES USING SURFACE DEACTIVATING AGENTS. | |
| ES8601309A1 (en) | Method of measuring biological ligands. | |
| JPS646864A (en) | Detection of biocomponent | |
| RU2029296C1 (en) | Magnet inspecting device | |
| CA2786237A1 (en) | A method for sensing a chemical | |
| JPH07111428B2 (en) | Laser magnetic immunoassay method and measuring apparatus | |
| JPS63315951A (en) | Laser magnetic immunoassay instrument | |
| JPS5420742A (en) | Concentration detecting method of developer | |
| SU1573410A1 (en) | Method of nondestructive inspection of ferromagnetic articles |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term | ||
| FPAY | Renewal fee payment (prs date is renewal date of database) |
Year of fee payment: 12 Free format text: PAYMENT UNTIL: 20080304 |