KR100829831B1 - Whitening cosmetic composition containing plant extract - Google Patents

Abstract

The present invention relates to a cosmetic composition for whitening, and more particularly to a skin anti-aging and whitening cosmetic composition comprising a plant extract selected from the group consisting of Sinseoran extract, Gaechocho extract and mixed extracts thereof. Since the composition of the present invention effectively inhibits tyrosinase activity and thus production of melanin pigments, which are essential for melanin biosynthesis, it can be usefully used as a cosmetic composition for preventing skin aging and whitening.

With new book, forget-me-not, skin whitening

Description

A skin whitening compositions comprising natural plant extract

1 is a diagram showing the tyrosinase activity inhibition rate of the plant extract of the present invention.

Figure 2 is a figure showing the melanin production inhibition rate of the plant extract of the present invention.

Figure 3 is a diagram showing the cytotoxicity of HaCaT cells of human keratinocytes of the plant extract of the present invention.

The present invention relates to a cosmetic composition for preventing skin aging and skin whitening, including a plant extract selected from the group consisting of Sinseoran extract, Gaecho extract and mixed extracts thereof.

Human skin color depends largely on the amount of melanin pigment contained in skin cells. People with high levels of melanin have brown or black skin, while people with very low levels of melanin cause vitiligo. Melanin pigment is produced from tyrosine by tyrosinase in pigment cells called melanocytes, which are present in the basal membrane of the skin. Melanin pigments play an important role in protecting the skin from excessive ultraviolet rays, thereby preventing skin damage and the occurrence of skin cancer. However, excessive production of melanin due to sunlight, hormonal changes, inflammation, drugs, etc. deposits skin pigments, which causes a variety of cosmetic problems. For example, skin pigments and freckles, which are hyperpigmented, are caused by the increased amount of melanin pigment, which means that the production of melanin pigment is promoted or the number of melanocytes is increased.

Therefore, recently, various whitening cosmetics and medicines for preventing and improving such problems have been developed and marketed. At present, in the development of a whitening agent for skin beauty, a method of reducing the generated melanin pigment by decolorization and a method of inhibiting the activity of tyrosinase, an enzyme that forms melanin pigment, are known. However, whitening agents using tocopherols and vitamins used to reduce the melanin pigment are known to have a slight decolorizing effect of the melanin pigment. Therefore, attention is being paid to inhibitors that inhibit the production of melanin by inhibiting the activity of tyrosinase. Tyrosinase is an enzyme that catalyzes the reaction of oxidizing monophenols into melanocytes of higher animals. By this enzyme, tyrosine is oxidized to dopaquinone, and melanin pigment is produced through a series of chemical reactions and oxidation. This melanin pigment is brown or black, which absorbs excessive light in vivo and thus adversely affects skin beauty.

In view of the fact that inhibiting the activity of tyrosinase enzymes can inhibit the production of melanin pigments, in the field of conventional cosmetics, natural plant extracts have been used as active inhibitors of tyrosinase as natural whitening extracts. Specifically, Korean Patent No. 70824 discloses a whitening cosmetic material containing a mulberry extract, and Korean Patent No. 84763 discloses a whitening cosmetic material containing an extract of Hojang-geun, and Korean Patent No. 122094 discloses a whitening cosmetic A whitening composition containing an extract is disclosed, and Korean Patent No. 253842 discloses a whitening cosmetic material containing melberin separated from mulberry. However, when the cosmetics are manufactured using the above components, there are disadvantages such as discoloration of the cosmetics due to oxidation by reaction with light during the manufacturing process or product storage. Therefore, additives such as antioxidants, UV absorbers, and metal chelates have been added to reduce such discoloration. However, when a large amount of such additives are used, there is a problem in skin stability, such as irritating the skin, and sufficient whitening effect as desired. The disadvantage is that you can't get it.

Phormium tenax , native to New Zealand, is 1 to 2 m high, with long, knife-shaped leaves agglomerate from the lateral rhizome. The leaves are pointed, 1 to 3 m long, and 5 to wide. It is more than 10 cm long, well-developed fiber, and is distributed in warm regions of Asia, including New Zealand and Australia. This plant may be planted or bonsai for ornamental purposes. The only hard fiber-derived plant that can be cultivated in temperate zones, yielding 12% fiber from leaves. The fiber is flexible, elastic and resistant to salting and salty, so it is used as a raw material for ship ropes, sails, rugs and paper (corrugated cardboard, cardboard, cardboard, etc.). However, the pharmacological efficacy of synthosis is not known yet.

Erigeron annuus ) is a naturalized plant native to North America belonging to the Asteraceae family, also called dwarf grass, broad-leaved hyacinth or deciduous herb. Even during winter, the leaves are alive, and in the next spring, they start to grow earlier than other plants and grow to a height of 30 to 100 cm. Run Young leaves are edible and used as compost. In oriental medicine, it is prescribed for colds, mucosa, lymphadenitis, infectious hepatitis, gastritis, enteritis or diarrhea (Kim Tae-Jung, Korea Plant Resources IV. Seoul National University Press, p.233-235 (1996); Doosan Global Encyclopedia). In addition, it has been reported that Ganoderma lucidum exerts highly mutually inhibitory effects on several crops (Lee Joong-ho, Lee Seung-yeop. Wonkwang University Journal Vol.17 (2): 107-139 (1983)). It is known that the separated substance can be used as a herbicide by inhibiting germination and growth of plants (Korean Patent No. 441597).

Therefore, the present inventors have studied Jeju native native plant extract to find a plant natural substance that is effective for skin whitening, and as a result, it was confirmed that the extract of Sinseoran and Ganoderma lucidum of the present invention inhibited tyrosinase activity and inhibited melanin production. The invention was completed.

Accordingly, it is an object of the present invention to provide a plant natural extract that has excellent skin anti-aging and skin whitening effects by inhibiting the activity of tyrosinase and inhibiting melanin production, and having no problem in stability.

Another object of the present invention is to provide a cosmetic composition comprising a plant extract having the above-described skin aging inhibitory and skin whitening efficacy.

As one embodiment, the present invention relates to a skin aging prevention and whitening cosmetic composition comprising a plant extract selected from the group consisting of cinnabar egg extract, Ganoderma lucidum extract, and mixed extracts thereof.

As used herein, the term “Phormium tenax” refers to a plant belonging to the family Liliaceae, which includes roots, stems, leaves, or fruits, including natural, hybrid, or varieties thereof. In addition, “Erigeron annuus” is a plant belonging to the Asteraceae family and includes roots, stems, leaves, or fruits, including its natural, hybrid, or varieties.

As used herein, the term "extract" refers to a substance having an activity of inhibiting tyrosinase activity and melanin production isolated from natural products. In the present invention, the extract is used as a meaning including not only the extract, but also its dry powder or any form formulated using the same.

In the present invention, the plant extract may be extracted using water, an organic solvent or a mixed solvent thereof, and preferably, may be extracted using an organic solvent. The extracted liquid may be used in liquid form or may be concentrated and / or dried.

When extracted with an organic solvent, methanol, ethanol, isopropanol, butanol, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, dichloromethane, N, N-dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol, or a mixed solvent thereof is used, and the active ingredient of the extract can be extracted at room temperature or warmed under conditions that are not disrupted or minimized. Depending on the organic solvent to be extracted, the degree of extraction and loss of the active ingredient of the extract may vary, so select an appropriate organic solvent. The extraction method is not particularly limited, and examples thereof include cold needle extraction, ultrasonic extraction, reflux cooling extraction, and the like.

Filtration is a process of removing the suspended solid particles from the extract, it may be used to filter the particles using cotton, nylon or the like, ultrafiltration, cryofiltration, centrifugal separation, etc., but is not limited thereto. It may also further comprise a separation process by various chromatography (chromatography according to size, charge, hydrophobicity or affinity).

Drying the filtrate includes, but is not limited to, lyophilization, vacuum drying, hot air drying, spray drying, reduced pressure drying, foam drying, high frequency drying, infrared drying, and the like. If desired, a process of grinding the final dried extract may be added.

In one specific embodiment, the cinnabar egg extract of the present invention, Ganoderma lucidum extract, and the mixed extract of Cinnaran egg and Ganoderma lucidum, three times to 20 times the weight (kg) by using dried cinnabar egg, Gaecho or mixed samples thereof as a powder Pears, preferably 3 to 15 times water, C ₁ to C ₄ lower alcohols or mixed solvents thereof, preferably ethanol or methanol solvents; 10 to 40 ° C., preferably 15 to 37 ° C. extraction temperature; A time of 5 hours to 15 days, preferably 3 days to 14 days; One to five times, preferably one to three times; The extract obtained by cold needle, ultrasonic extraction, reflux cooling extraction, preferably cold needle extraction method was obtained by filtration and concentration under reduced pressure.

Sinseoran extract, Gaechocho extract or a mixture extract thereof obtained by the above production method effectively inhibits the activity of tyrosinase and melanin production, prevents skin aging and exerts a skin whitening effect.

In addition, the plant extract can be used safely in the cosmetic composition of the present invention because there is little toxicity and side effects.

Sinseoran extract, Gaechocho extract or a mixture extract thereof obtained by the above production method is 0.0001 to 5% by weight, preferably 0.001 to 5% by weight, more preferably 0.001 to 3% by weight based on the total weight of the composition of the present invention. It may be included as.

The composition of the present invention can be used in a variety of cosmetics, face wash and shampoo for skin aging and whitening effect. Products to which the composition can be added include, for example, cosmetics such as various creams, lotions, skins, and the like, shampoos, rinses, cleansing agents, face washes, soaps, treatments, and essences.

The cosmetic composition of the present invention may further include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extracts.

The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be formulated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-α tocopherol, d-α tocopherol, d-δ tocopherol), and the like. And derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-α tocopherol acetate, dl-α tocopherol vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl Ethers, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be incorporated into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate, purified from these seaweed extracts, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

The cosmetic composition of this invention may mix | blend the other component normally mix | blended with cosmetics as needed. Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylol propane, triisostearic acid trimethylol propane, tetra 2-ethylhexanoic penta erythritol Cetyl caprylate, lauric acid decyl, hexyl laurate, decyl myristin, myristin acid myristyl, myritic acid cetyl, stearyl stearate, decyl oleate, rininooleic acid , Isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecate oleate, octylate decyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2 -Cetostearyl ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol dicacapate, capric acid Propylene glycol phthalic acid, neopentyl glycol dicapric acid, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl tritridecyl acid, glyceryl triisopalmitinate, glyceryl triisosulfate, octyldode neopentane Sil, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isstearyl isostearate, Sothete octylate, polyglycerol oleate, polyglycerine isostearate, triisocetyl citrate, triisoalkyl citrate, triisoctyl citrate, lauryl lactate, myritic lactate, octyl lactate, octyl lactate, tricitric acid Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipic acid, diisopropyl sebacinate, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-Steloylhydroxystearate stearyl, 12-stealo Esters such as monohydroxystearic acid isostearyl; and the like.

Examples of the hydrocarbon-based oils and fats include hydrocarbon oils such as squalene, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, and vaseline.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane, methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Perfluoro polyether etc. are mentioned as fluorine-based fats and oils.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

Water-soluble low molecular humectants include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol (polymerization degree n = 2 or more), polypropylene glycol (Polymerization degree n = 2 or more), polyglycerol (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

Nonionic surfactants include self-emulsifying glycerin monostearate, propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerol fatty acid esters, sorbitan fatty acid esters, POE (polyoxyethylene) sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE / POP (polyoxyethylene / polyoxypropylene) copolymer, POE / POP alkyl ether, polyether modified silicone, lauric acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

As anionic surfactant, fatty acid soap, (alpha)-acyl sulfonate, alkyl sulfonate, alkyl allyl sulfonate, alkyl naphthalene sulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphorus salt, alkylamide Phosphate, alkyloylalkyltaurin salt, N-acylamino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, sodium alkyl sulfoacetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester, etc. Can be.

As cationic surfactant, alkyl trimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments, such as a silk powder, a cellulose, CI pigment yellow, and CI pigment orange, and the composite pigment of these inorganic pigments and an organic pigment, etc. are mentioned.

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-β-alanine calcium, N-lauroyl-β-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-acyl basic amino acids, such as N (epsilon) -lauroyl-L- lysine, N (epsilon) -palmitolysine, N (alpha)-partoylol nitin, N (alpha)-lauroyl arginine, and N (alpha) -cured fatty acid acyl arginine; N-acyl polypeptides, such as N-lauroyl glycyl glycine; α-amino fatty acids such as α-aminocaprylic acid and α-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono2-ethylhexaneglyceryl, isopropyl paramethoxy cinnamic acid, diisopropyl diisopropyl cinnamic acid ester mixture, urokane Acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenonedisulfonic acid sodium salt, dihydroxybenzophenone, Tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p- (carbo-2'-ethylhexyl-1'-oxy) -1, 3,5-triazine, 2- (2-hydric When the like can be mentioned 5-methylphenyl) benzotriazole.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

Moreover, the compounding component which may be added other than this is not limited to this, Although any said component can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5 weight% with respect to a total weight, More preferably, 0.01 to 3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

Components included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the extract or compound as an active ingredient, for example, such as stabilizers, solubilizers, vitamins, pigments and flavorings Phosphorus aids and carriers.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, and includes, for example, milky lotion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic, and the like.

Specifically, the cosmetic composition of the present invention skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in more detail with reference to Examples. The following examples are only intended to illustrate the present invention in more detail and are not intended to limit the present invention thereto.

Example  1: manufacturing method of plant extract

1-1. Preparation of Water Soluble Extract

Completely dried, 1 kg of Cinderella, Ganoderma lucidum, and 1 kg of Cinderella orchid and Ganoderma lucidum mixed sample was added to 3 l of distilled water and extracted for 14 days at 15 to 37 ° C. in an extractor equipped with a cooling condenser. The extract was filtered through a 400 mesh filter cloth, left to mature at 5 to 15 ° C. for 7 days, and then filtered using Whatman No. 2 filter paper. These plant extracts were concentrated under reduced pressure in a distillation apparatus equipped with a cooling condenser to obtain 29 g, 30 g, and 29 g (dry weight), respectively.

1-2. Preparation of Ethanol Soluble Extract

Completely dried, 1 kg of Cinderella, Gazelweed, and 1 kg of Cinderella and Gazelweed mixed samples were placed in 3 l of ethanol and extracted for 14 days at 15 to 37 ° C. in an extractor equipped with a cooling condenser. The extract was filtered through a 400 mesh filter cloth, left to mature at 5 to 15 ° C. for 7 days, and then filtered using Whatman No. 2 filter paper. These plant extracts were concentrated under reduced pressure in a distillation apparatus equipped with a cooling condenser to obtain 31 g, 39 g, and 37 g (dry weight), respectively.

1-3. Preparation of Methanol Soluble Extract

Completely dried, 1 kg of Cinderella, Ganoderma lucidum, and 1 kg of Cinderella and Cauliflower mix samples were placed in 3 l of methanol and extracted for 14 days at 15 to 37 ° C. in an extractor equipped with a cooling condenser. The extract was filtered through a 400 mesh filter cloth, left to mature at 5 to 15 ° C. for 7 days, and then filtered using Whatman No. 2 filter paper. These plant extracts were concentrated under reduced pressure in a distillation apparatus equipped with a cooling condenser to obtain 38 g, 41 g, and 39 g (dry weight), respectively.

Experimental Example  1: of plant extract Tyrosinase  Inhibitory effect

The tyrosinase inhibitory effect was measured on the extract of Sinseoran, Gaechochoe extract, Sinseoran and Gaechocho mixed extract, and licorice extract, lettuce, and arbutin obtained in Example 1-2. Tyrosinase is an enzyme purified from potatoes and mushrooms. In this experiment, Sigma company extracted from mushrooms was used. First, tyrosine, a substrate, was dissolved in sodium phosphate buffer (0.1M, pH6.8) to make a solution having a concentration of 0.3 mg / ml, and the solution was added to a test tube with 334 µl, and then sodium phosphate buffered again. 576 μl of solution (0.1M, pH6.8) was added. The dried plant extract of Example 1-2 was dissolved in methanol to 10 mg / ml, and diluted to 5, 1.5, 1 mg / ml, 500,150, and 100 µg / ml using methanol, respectively. 70 μl of each of these diluted extract samples was added to the in vitro solution and reacted in a 37 ° C. thermostat for 10 minutes.

The control group was put in 70 μl of methanol instead of the plant extract of the present invention. 20 µl of 1700 units / ml tyrosinase solution was added to the reaction solution of the experimental group and the control group so that the total volume was 1 ml, and the reaction was continued for 30 minutes in a 37 ° C thermostat. The test tube containing the reaction solution was placed in iced water and quenched to stop tyrosinase activity, and then absorbance at 470 nm was measured with a photospectrometer.

The blank of the experimental group was a reactant excluding tyrosinase solution, ie, tyrosine, seaweed and plant extracts, and sodium phosphate buffer, and the blank of the control group was added with methanol instead of the plant extract in the blank of the experimental group. When measuring the inhibitory effect of tyrosinase activity on arbutin, arbutin was dissolved in sodium phosphate solution to a concentration of 10 mg / ml, and then 5, 1.5, 1 mg / ml, 500, 150 using sodium phosphate buffer solution. And diluted to 100 µg / ml to make the experimental group. The blank of the experimental group of arbutin is the reactant excluding tyrosinase, ie tyrosine, arbutin solution, sodium phosphate buffer solution. The control group of arbutin was a sodium phosphate buffer solution instead of the arbutin solution, and the blank of the arbutin control group was a reaction product except tyrosinase solution, that is, tyrosine and sodium phosphate buffer solution. The above is summarized in Table 1 below.

Material used Experimental group Experimental  Blank Control Blank of Control Tyrosine (0.3 mg / ml) 334 μl 334 μl 334 μl 334 μl Sodium Phosphate Buffer Solution (0.1M, pH6.8) 576 μl 596 μl 576 μl 596 μl sample 70 μl 70 μl 70 μl 70 μl Tyrosinase solution (1700 units / ml) 20 μl - 20 μl - Note) Total volume 1 ml

Inhibition rate of tyrosinase to plant extracts and controls was determined using the following Experimental formula 1. The experimental results are shown in Table 2.

[Equation 1]

% Tyrosinase inhibition = 100-{[(A-B)-(C-D)] / (A-B)} X 100

A: absorbance of the control group B: blank absorbance of the control group

C: absorbance of the experimental group D: blank absorbance of the experimental group

sample Concentration of the sample that inhibits tyrosinase activity by 50% (IC 50: μg / ml) Forget-me-not extract 278.56 Sinceran Extract 4.62 Camembert + Shinseoran Extract 4.51 White skin 8.17 Licorice extract 326.52 Arbutin 156.22

As shown in Table 2, Sinseoran extract and Sinseoran and Gaechocho mixed extract was 2 times higher than that of baekryepi, 20 times of arbutin, about 40 times higher than licorice extract. In addition, Gaechocho extract also showed a whitening effect about 1.5-3.0 times higher than licorice extract, a natural whitening agent.

Experimental Example  2: Inhibitory Effect of Plant Extracts on Melanin Production

Rat pigment cells derived from C57BL / 6 mice were placed in Dulbeccos modified Eagles media (DMEM) with 10% PBS (fetal bovine serum), 10 nM 12-O-tetradecanoylphorball-13-. Incubated at 37 ° C. and 5% CO ₂ in medium containing acetate (12-O-tetradecanoylphorbol-13-acetate) and 1 nM cholera toxin.

After culturing the cells at a concentration of 10 ⁵ cells / well in 24 well plates and confirming cell adhesion, the plant extracts of Example 1-2 were combined with the cell culture medium as shown in Table 3 to obtain a final concentration of 0.001. Cultured for 3 days at% to 0.004%. The culture solution was removed, washed with PBS, and the cells were dissolved with 1N NaOH and absorbance was measured at 400 nm. Using the cells without the test substance as a control, the whitening effect was measured by measuring the degree of inhibition of melanin production of the test substance compared to the melanin content in the control.

In addition, as a comparative example, the whitening effect of the lettuce extract, licorice extract and koji acid extracted by the same method using C57BL / 6 mouse pigment cells was measured by the same method. The melanin production inhibitory effect of the plant extract obtained from it is shown in Table 3 below.

[Equation 2]

Melanin production inhibition rate (%) = 100- (absorbance of each extract / absorbance of control x100)

As can be seen in Table 3, the extract of Sinseoran and Ganoderma lucidum of the present invention showed very high melanin production inhibitory effect at a low concentration of 0.001%. In addition, these extracts were already excellent in whitening effect even at low concentrations compared to the whitening skin, licorice extract, koji acid, etc., which are known to have excellent whitening effect.

sample Concentration (%) Melanin production inhibition rate (%) Forget-me-not extract 0.001 19.4 Sinceran Extract 0.001 72.9 Camembert + Shinseoran Extract 0.001 81.5 White skin 0.001 30.2 Licorice extract 0.001 3.2 Kojisan 0.001 2.0

Experimental Example  3: Cytotoxicity Evaluation of Plant Extracts

MTT experiments were carried out using HaCaT (Korean Cell Line Bank) and human normal fibroblasts (Pacific Co., Ltd.), which are human keratinocyte lines, for cytotoxic effects of natural plant extracts. In the case of human normal fibroblasts and HaCaT, Cintran egg extract, Ganoderma lucidum extract, and Cinnabar egg and Cauliflower mixed extract did not show cytotoxicity at 1 μg / ml of natural extract (FIG. 3).

Experimental Example  4: safety verification experiment on human skin

4-1. Contains natural extracts External skin preparations  Produce

In order to confirm whether the natural plant extracts of the present invention, which are found to be excellent in whitening effect as described above, are safe for human skin, an external preparation for skin containing the natural plant extracts of the present invention was prepared and skin safety verification experiments were performed.

Skin external preparations containing natural plant extracts were prepared by the ingredient contents of Table 4 below. In the following Table 4, the control is an external preparation for skin that does not include a natural extract.

For the preparation of external skin preparations, purified water, glycerin and butylene glycol were mixed and dissolved at 70 ° C. (water part), and the other components except for the three components and trimethanolamine were dissolved at 70 ° C. (oil part). The oil part was added to the water part and stirred with a homomixer (Tokushu Kika, Japan) to emulsify first, to which trimethanolamine was finally added. After removing the bubbles generated in the mixed solution, and cooled to room temperature to prepare a skin external preparation.

ingredient content (%) Control Test group Purified water 72 72 glycerin 8.0 8.0 Butylene glycol 4.0 4.0 Cinnabar egg extract, forget-me-not extract, or Cinderella egg and forget-me-not extract 0 1.0 Caprylic Capri Triglyceride 8.0 8.0 Squalane 5.0 5.0 Cetearyl Glucoside 1.5 1.5 Sorbitan stearate 0.4 0.4 Cetearyl Alcohol 1.0 1.0 Trimethanolamine 0.1 0.1 Gross weight 100 100

4-2. Skin accumulation stimulation test

Plant extracts of the present invention are irritated to the skin by performing a total of nine 24-hour cumulative blisters on the upper forearm every other day for 30 healthy adults using each skin preparation prepared in Example 4-1. It was measured whether or not giving.

The patching method used a fin chamber (Finn chamber, Epitest Ltd, Finland). 15ul of each said skin external preparation was dripped at the chamber, and the patch was performed. The degree of response to the skin each time was scored using the following formula, and the results are shown in Table 5 below.

[Equation 3]

Average responsiveness = [[response index x responsiveness / total number of subjects x highest score (4 points)] x 100] ÷ number of tests (9 times)

At this time, ± 1 point, + 2 points, + + 4 points in the reactivity, it is determined as a safe composition when the average reactivity is less than 3.

Test substance Number of subjects with response Average reactivity 1 week 2 weeks 3 weeks Primary Secondary 3rd 4th 5th 6th 7th 8th 9th ± + + + ± + + + ± + + + ± + + + ± + + + ± + + + ± + + + ± + + + ± + + + Control One - - --- --- --- --- --- --- --- --- 0.11 Mixed extract 2 1- --- --- --- --- --- --- --- --- 0.33 Forget-me-not extract 1 1- --- --- --- --- --- --- --- --- 0.18 Sinceran Extract One - - --- --- --- --- --- --- --- --- 0.11 Inspector 30 30 30 30 30 30 30 30 30

As shown in Table 5, the test group did not show any special side effects. When calculated according to the empirical formula 3, the three kinds of average reactivity including the extract of the new book is 0.11 to 0.33, all four were determined to be a safe composition because it is 3 or less.

Experimental Example  5: Acute Oral Toxicity Test

An acute oral toxicity test was conducted to determine whether natural extracts could be safely applied to food. Twenty five to six week old SPF SD rats were used as an experimental animal under the acute toxicity test under the following conditions.

Temperature and humidity conditions: temperature 22 degrees +/- 2 degrees. Relative Humidity 50 +/- 10%

Contrast cycle: Fluorescent lighting (08:00 light-20:00 light)

Illuminance: 200-300Lux

Free treatment of UV-treated drinking water

A test sample was prepared by diluting the test substance with sterile distilled water, and the same amount was added using sterile distilled water as a control.

On the day of administration, the general condition is observed every hour up to 4 hours, and once a day from the day after the administration, the general condition changes, poisoning symptoms, mobility, appearance, autonomic nerves, and the presence of dead animals are carefully observed. Pathological anatomical verification was performed to determine the toxicity. LD ₅₀ was 2700 mg / kg BW, and all three natural extracts were judged to be non-toxic.

Production Example

Production Example  1: Nutrition Cream

As follows, the nutrient cream containing the natural extract obtained in Example 1 was prepared according to a conventional method.

ingredient Content (% by weight) Natural extracts 0.1 Liquid paraffin 6.0 Squalane 4.0 Sorbitan sesquioleate 0.5 Polysorbate 60 1.5 beeswax 10.0 Capric & Capric Triglycerides 5.0 Concentrated glycerin 6.0 Propylene glycol 3.0 Butylene glycol 2.0 Triethanolamine 0.2 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Balance

Production Example  2: nutrient lotion

As follows, nutrient lotion (lotion) containing the natural extract obtained in Example 1 was prepared according to a conventional method.

ingredient Content (% by weight) Natural extracts 0.1 ethanol 1.0 Stearyl alcohol 1.0 Glycerin Monostearate 3.0 Sorbitan monostearate 1.0 Polyoxyethylene sorbitan monooleate 2.0 vaseline 2.0 Liquid paraffin 6.0 Squalane 5.0 Octyldodecyl Myristate 5.0 glycerin 5.0 1,3-butylene glycol 5.0 Carboxy Vinyl Polymer 0.3 Triethanolamine 0.3 antiseptic a very small amount Spices a very small amount Purified water Balance

Production Example  3: Flexible Cosmetics

As described below, the softener (skin) containing the natural extract obtained in Example 1 was prepared according to a conventional method.

ingredient Content (% by weight) Natural extracts 0.1 ethanol 10.0 Propylene glycol 3.0 Concentrated glycerin 2.0 Butylene glycol 2.0 Carboxy Vinyl Polymer 0.1 Triethanolamine 0.1 antiseptic a very small amount Spices a very small amount Pigment a very small amount Purified water Balance

Production Example  4: Essence

As follows, the essence containing the natural extract obtained in Example 1 was prepared according to a conventional method.

ingredient Content (% by weight) Natural extracts 0.1 ethanol 5.0 Pluylene glycol 5.0 Polyethylene Glycol 1500  5.0 Hydroxyethyl cellulose 0.5 Polyoxyethylene nonylphenyl ether 0.7 glycerin 10.0 1,3-butylene glycol 10.0 Carboxy Vinyl Polymer 0.5 Triethanolamine 0.5 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Balance

Production Example  5: Massage cream

As follows, the massage cream containing the natural extract obtained in Example 1 was prepared according to a conventional method.

ingredient Content (% by weight) Natural extracts 0.1 Liquid paraffin 40.0 Pluylene glycol 2.0 Squalane  5.0 Sorbitan sesquioleate 0.8 Polysorbate 60 1.5 Capric & Capric Triglycerides 4.0 Concentrated glycerin 6.0 Butylene glycol 4.0 beeswax 10.0 Triethanolamine 0.2 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Balance

Production Example  6: pack

As follows, a pack containing the natural extract obtained in Example 1 was prepared according to a conventional method.

ingredient Content (% by weight) Natural extracts 0.1 ethanol 5.0 Allantoin 0.2 Nonylphenyl Ether 0.3 Sodium Carboxymethyl Cellulose 0.2 Polyvinyl alcohol 13.0 antiseptic a very small amount Pigment a very small amount Spices a very small amount Purified water Balance

As described above, the cosmetic composition containing the extract of Sinseoran, Gaechocho extract or mixed extract thereof of the present invention is excellent in inhibiting the activity of tyrosinase and inhibiting melanin production, and thus may be usefully used as a cosmetic composition for preventing skin aging and whitening Can be.

Claims (6)

A cosmetic composition for whitening comprising a sour extract. The cosmetic composition for whitening according to claim 1, wherein the extract of Sinseoran is obtained by extracting Sinseoran with water, methanol, ethanol or a mixed solvent thereof. According to claim 1, wherein the composition is a cosmetic composition for whitening further comprising the extract of Ganoderma lucidum extract in Sinseoran. delete The whitening cosmetic composition according to any one of claims 1 to 3, wherein the extract of Sinseoran or a mixed extract of Sinseoran and Ganoderma lucidum is contained in an amount of 0.001 to 1.0% by weight based on the total weight of the composition. According to any one of claims 1 to 3, wherein the cosmetic composition is a skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, Whitening cosmetic composition, characterized in that it is formulated into any one selected from the group consisting of hand cream, foundation, essence, nutrition essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

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