KR102123645B1 - Cosmetic composition with Angelica japonica extract - Google Patents

Abstract

The present invention relates to a cosmetic composition containing a Gaetgang extract, and relates to a cosmetic composition having antioxidant, skin barrier strengthening, skin irritation relaxation, and skin wrinkle improvement effects.

Description

Cosmetic composition with Angelica japonica extract}

The present invention relates to a cosmetic composition containing a Gaetgang Liver Extract, and relates to a cosmetic composition having antioxidant, skin barrier strengthening, and skin irritation alleviating effects.

Fine dust is a very small material that is invisible to our eyes, and refers to a particulate matter with a diameter of 10 µm or less that floats or floats in the air for a long time. Fine dust is an artificial contaminant by fossil fuels used in factories, unlike yellow dust. It is composed of ionic components such as nitrate (NO3-), ammonium (NH ₄ ⁺ ) and sulfate (SO ₄ ^2- ), carbon compounds, and metal compounds consist of. When exposed to fine dust for a long period of time, immunity rapidly decreases, which can lead to exposure to various diseases such as cardiovascular disease, skin disease, and eye disease, as well as respiratory diseases such as cold, asthma, and bronchitis. In particular, ultra-fine dust having a diameter of 2.5 µm or less can easily penetrate into the human bronchi and deep into the lungs and cause various diseases.

Fine dust affects not only inflammatory skin diseases such as psoriasis, atopic dermatitis, and acne, but also skin aging, hair loss, and skin cancer. Since the fine dust is up to 20 smaller than the size of the pores, it can easily penetrate into the skin, and as the oils and entanglements of the skin accumulate, the skin barrier breaks down and metal harmful substances such as arsenic, cadmium, and lead are introduced into the skin. It can cause trouble or inflammatory reaction. Accordingly, the skin immune function is weakened, the protective function of the skin barrier is lost, and serious skin disease may be caused. It is also known that increased inflammatory cytokines caused by fine dust and increased free radicals in the body increase the inflammatory response, wrinkle formation, and itching in skin cells, and seriously affect the development of skin cancer.

Korean Registered Patent No. 10-176415 (2019.05.02.) discloses a composition for external application for skin irritation or skin irritation that contains an ipop tree extract as an active ingredient. Korean Patent Registration No. 10-1956886 (2019.03.05.) describes a cosmetic composition for improving skin damaged by fine dust or yellow dust.

The present invention is to develop and provide a cosmetic composition capable of alleviating skin aging and skin irritation caused by fine dust.

The present invention provides a cosmetic composition for improving skin aging containing an extract of Angelica japonica.

In the present invention, the skin aging may preferably be due to fine dust.

In addition, the present invention provides a cosmetic composition for strengthening the skin barrier containing an extract of Angelica japonica.

In the present invention, the skin barrier may preferably be a skin barrier damaged due to fine dust.

In addition, the present invention provides a cosmetic composition for relieving skin irritation containing an extract of Angelica japonica.

In the present invention, the skin irritation may be preferably caused by fine dust.

In addition, the present invention provides a cosmetic composition for improving wrinkles containing an extract of Angelica japonica.

In the present invention, the skin wrinkles may preferably be due to fine dust.

In the present invention, the cane extract is preferably water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, propylene glycol, butylene glycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloro It may be obtained using an extraction solvent selected from the group consisting of methane, hexane and mixtures thereof.

In the present invention, it is preferable to contain the cane extract, preferably 0.001 to 30% by weight relative to the total weight of the cosmetic composition.

The Gaetgang Liver Extract of the present invention has the effect of inhibiting skin aging according to the antioxidant effect, and has the effect of strengthening the skin barrier, improving skin wrinkles and alleviating skin irritation.

The present invention provides a cosmetic composition containing a Gaetgang extract, antioxidant, skin barrier strengthening, skin wrinkle improvement, skin irritation relaxation effect.

1 is an experimental result showing the DPPH radical scavenging effect of the Gaetgang livestock extract.
Figure 2 is the experimental results showing the effect of the expression of Claudin-1 and Desmoglein-2 of the Gaetgang live extract.
3 is an experimental result showing the effect of Nrf2 expression of the Gaetgang live extract.
Figure 4 is an experimental result showing the effect of inhibiting the MMP-1 expression of the Gaetgang live extract.
5 is an experimental result showing the NO inhibition rate of the Gaetgang livestock extract.
6 is an experimental result showing the inhibitory effect of TNF-α and IL-8 expression of the cosmetic composition containing the Gaetgang extract.

The present invention provides a cosmetic composition for improving skin aging, strengthening skin barrier, reducing skin irritation, and improving skin wrinkles, containing an extract of Angelica japonica . In the present invention, the skin aging, skin irritation, and skin wrinkles may preferably be caused by fine dust, and the skin barrier may be a skin barrier damaged by fine dust.

Angelica japonica is a perennial plant that branches from the top of the stem and has a yellowish white juice and dark purple stripes on the outside. The flowers are white and bloom in July-August and grow well on the beach. It is also used as a remedy for physical weakness, headache, menstrual irregularities, and abdominal pain.

In the present invention, the canine extract, can use a variety of extraction methods commonly used in the art to which the present invention pertains, preferably water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, propylene glycol, butyl It can be obtained using an extraction solvent selected from the group consisting of renglycol, glycerin, acetone, ethyl acetate, chloroform, butyl acetate, diethyl ether, dichloromethane, hexane and mixtures thereof. More preferably, it is preferable to extract using a 70% (v/v) ethanol aqueous solution of 10 times the weight of the gland stick. In order to use the gaet extract, it can be dissolved in an appropriate solvent for use as a cosmetic composition. Preferably, it is preferable to use it by dispersing and dissolving it using one or more solvents selected from purified water, ethanol, butylene glycol, and propylene glycol. .

The Gaetgang Liver Extract of the present invention has an antioxidant effect according to the free radical scavenging effect, and thus it was confirmed that it has the effect of inhibiting skin aging.

In addition, it was confirmed that the intestinal lubricity extract of the present invention promotes the expression of Claudin-1 and Desmoglein-2, thereby enhancing skin barrier. Claudin-1 and Desmoglein-2, which are trans-membrane proteins constituting the skin barrier, have reduced expression due to fine dust, and thus a problem that the skin barrier is weakened may occur, but the intestinal lubrication of the present invention It was confirmed that the cosmetic composition containing the extract exerts a strengthening effect on the skin barrier by increasing the expression of Claudin-1 and Desmoglein-2, whose expression is reduced by fine dust.

In addition, it was confirmed that the gaet gang extract of the present invention has an effect of protecting and detoxifying the human body from oxidative stress by promoting Nrf2 expression.

In addition, it was found that the intestinal lubricity extract of the present invention inhibits the expression of MMP-1. MMP-1 can be promoted by fine dust expression, but it was confirmed that the cantilever extract of the present invention effectively suppresses the expression of MMP-1 caused by fine dust and has the effect of improving wrinkles.

In addition, the Gaet ganglung extract of the present invention has an antioxidant effect according to the NO inhibitory effect, and thus it was confirmed that it has the effect of inhibiting skin aging.

On the other hand, in the present invention, it is preferable to contain the cane extract, preferably 0.001 to 30% by weight relative to the total weight of the cosmetic composition. When included in less than 0.001% by weight, the effect of exerting various skin functions confirmed in the present invention is insignificant, and when it exceeds 30.0% by weight, it is not economical because the efficiency is less than the material input.

The cosmetic composition containing the gland extract of the present invention has an effect of improving skin wrinkles, as shown in the experiment below, and has an effect of alleviating skin irritation by significantly reducing the expression levels of the inflammatory cytokines TNF-α and IL-8. Was confirmed. Skin wrinkles or inflammatory cytokines can be caused by fine dust, and it was confirmed that the cosmetic composition containing the cannula extract of the present invention has a relieving effect against irritation caused by fine dust.

On the other hand, the cosmetic composition of the present invention, for example, solution, suspension, emulsion, paste, lotion, gel, water-soluble liquid, cream, essence, surfactant-containing cleansing, oil, oil-in-water (O/W) type and oil Any one of the basic cosmetic formulations selected from heavy water (W/O) type; skin; Lotion; Eye cream; Soothing gel; Ointment; Formulation for mask packs; Body wash formulations; Peeling gel; Water-in-oil and water-in-oil makeup bases; foundation; Skin cover; Lipstick, lip gloss, face powder, two-way cake, eye shadow, cheek color, and eyebrow pencils. Scalp formulations; It may be any one selected from.

In addition, the cosmetic composition of the present invention may contain auxiliaries commonly used in the cosmetic field, such as hydrophilic or lipophilic actives, preservatives, antioxidants, solvents, fragrances, fillers, blockers, pigments, odorants, dyes, and the like. The amount of these various adjuvants is an amount commonly used in the art, for example, 0.001 to 30% by weight based on the total weight of the composition. However, in any case, the auxiliary agent and its ratio will be selected so as not to adversely affect the desirable properties of the cosmetic composition according to the present invention.

On the other hand, in the cosmetic composition of the present invention, when the formulation is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica , Talc or zinc oxide may be used.

In addition, in the cosmetic composition of the present invention, when the formulation is a solution or an emulsion, a solvent, a solubilizing agent or an emulsifying agent is used as a carrier component, for example water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, Fatty acid esters of benzoate, propylene glycol, 1,3-butylene glycol oil, glycerol aliphatic esters, polyethylene glycol or sorbitane can be used.

In the cosmetic composition of the present invention, when the formulation is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester The same suspending agents, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, etc. can be used.

On the other hand, in the cosmetic composition of the present invention, when the formulation is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier performance, especially in the case of a spray. Additionally it may include propellants such as chlorofluorohydrocarbon, propane/butane or dimethyl ether.

On the other hand, in the cosmetic composition of the present invention, when the formulation is a surfactant-containing cleansing, as the carrier component, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, sarcosinate, Fatty acid amide ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

In addition, in the cosmetic composition of the present invention, in the case of a surfactant-containing cleansing formulation or a surfactant-free cleansing formulation or soap, after applying to the skin, it may be wiped off, peeled off, or washed with water. In one example, the soap is liquid soap, powdered soap, solid soap and oil soap, and the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, a cleansing towel and a cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream, Cleansing lotions, cleansing water and cleansing gels, but are not limited thereto.

In addition, the cosmetic composition of the present invention can be used in combination with other cosmetic compositions other than the present invention. In addition, the cosmetic composition according to the present invention may be used according to a conventional method of use, and the number of times of use may be varied according to a user's skin condition or taste.

Hereinafter, the present invention will be described in more detail in the following Examples and Experimental Examples. However, the scope of the present invention is not limited to the following examples and experimental examples, and includes all modifications of equivalent technical ideas.

[Example 1: Preparation of Gaetgang Liver Extract]

After crushing the mudguard outpost, 100g was extracted in 10% of 70% (v/v) ethanol aqueous solution for 3 days at room temperature. The extract obtained by extraction was filtered with Whatman #5 filter paper. The filtered extract was concentrated under reduced pressure and freeze-dried at 50° C. or lower to obtain 18.6 g of cane extract. Dispersed and dissolved using at least one solvent selected from purified water, ethanol, butylene glycol, and propylene glycol so that the reduced pressure concentration and freeze-dried product contained 0.001 to 30.0 wt% was used in the following experiment.

[Production Examples 1 to 17: Preparation of Gaetgang Liver Extract Using Various Extraction Solvents]

Using the extracting solvent according to Table 1 below, a gaetganglu extract was prepared in the same manner as in Example 1. The dry weight of the final extract obtained is shown in Table 1 below.

Extraction solvent Dry weight of final extract (g) Example 1 70% (v/v) ethanol 18.6 Preparation Example 1 10% (v/v) ethanol 1.5 Preparation Example 2 20% (v/v) ethanol 4.3 Preparation Example 3 30% (v/v) ethanol 7.6 Preparation Example 4 40% (v/v) ethanol 8.2 Preparation Example 5 50% (v/v) ethanol 12.5 Preparation Example 6 60% (v/v) ethanol 14.2 Preparation Example 7 80% (v/v) ethanol 17.3 Preparation Example 8 90% (v/v) ethanol 16.5 Preparation Example 9 100% (v/v) ethanol 13.1 Preparation Example 10 Methanol 11.3 Preparation Example 11 n-propanol 10.2 Preparation Example 12 Isopropanol 7.2 Preparation Example 13 2-butanol 4.3 Preparation Example 14 Acetone 5.1 Preparation 15 chloroform 2.1 Preparation 16 Ethyl acetate 4.7 Preparation Example 17 1,3-butylene glycol 3.2

[Experimental Example 1: Confirmation of anti-oxidation effect of Gaetgang livestock extract]

In this experimental example, it was intended to confirm the antioxidant effect of the Gaetgang livestock extract prepared in Example 1. Among the antioxidant measurement methods, a typical DPPH radical is dissolved in an organic solvent and has a maximum absorbance at 560 nm. At this time, the antioxidant substance extinguishes the DPPH radical to lose its own color and turn yellow.

61.88mg of 2,2-di(4-tert-octylphenyl)-1-picrylhydrazyl free radical (Aldrich Chem. Co., MW=618.76) was dissolved in methanol to prepare a 0.1 mM DPPH solution. 0.15 ml of 0.1 mM DPPH solution and 0.15 ml of cantilever extract were added to a 96 well-plate, stirred, and incubated at 25° C. for 10 minutes, and absorbance (St) at 560 nm was measured. Blank of the sample solution was measured in the same manner as when measuring the absorbance (St), except that methanol was used instead of the 0.1 mM DPPH solution to measure absorbance (So). In the blank test, the absorbance (Bt) was measured in the same manner as when measuring the absorbance (St), except that distilled water was used instead of the water extract. The blank of the blank test was measured in the same manner as when measuring the absorbance (St), except that methanol was used instead of the 0.1 mM DPPH solution and distilled water was used instead of the cane extract. Did.

The results of the free radical scavenging rate (%) were calculated by Equation 1, and the results are shown in FIG. 1.

St: absorbance at 560 nm after free radical scavenging of the sample

So: Absorbance at 560 nm before reaction when free radicals are not added to the sample

Bt: Absorbance at 560 nm after free radical scavenging of the blank test solution

Bo: Absorbance at 560 nm before reaction when free radicals of the test solution are not added

As shown in Figure 1, it was confirmed that the anti-oxidant effect of the Gaetgang extract of the present invention is concentration-dependent. Accordingly, it was judged that the gaet gang extract of the present invention exerts an excellent skin anti-oxidation effect, thereby inhibiting skin aging.

[Experimental Example 2: Confirmation of Claudin-1 and Desmoglein-2 expression effects of Gaetgang Liver Extract]

Proteins that make up the skin barrier include claudin, a trans-membrane protein, and desmoglein-2 and symplkin. Among the various proteins, claudin's ring-shaped structure is connected to fill the space between cells, so it can effectively block the path of movement between cells of external stimuli. This tight junction is known to perform a complementary function with the stratum corneum as a skin barrier. In this experiment, we tried to confirm the effect of the expression of Claudin-1 and Desmoglein-2 of the Gaetgang extract.

Human-derived keratinocytes (HaCaT) cells were dispensed into 3×10 ⁵ cells/well in a 6 well plate, and the cells were stabilized for 1 day in a 37° C., 5% CO ₂ cell incubator. Cells were collected using a scraper after treating fine dust (50 ppm) and 10, 30, and 100 µg/ml of Gaet River Extract, respectively, and reacting for 6 hours. Protein was separated using 1x cell lysis buffer, and the protein was quantified by BCA assay, and a certain amount of protein was electrophoresed on a 10% SDS-PAGE gel. The isolated protein on the gel was transferred to the PVDF membrane and blocked for 1 hour using 5% skim milk. After washing 3 times with 1x TTBS solution and reacting the primary antibody (claudin-1, desmoglein-2, Cell signaling, USA) over night at 4° C., the secondary antibody bound with HRP is 2 at room temperature. Time reaction. Thereafter, washing was performed 3 times with 1x TTBS and the ECL solution was reacted to detect a band with a Chmi Doc instrument. Claudin-1 and Desmoglein-2 expression levels were calculated by quantifying the expression of beta-actin, and quantified using Image J 1.47 software. The experimental results are shown in FIG. 2.

As shown in FIG. 2, it was confirmed that the cannula extract of the present invention exerts a strengthening effect on the skin barrier by increasing the expression of Claudin-1 and Desmoglein-2 in which the expression level is reduced by fine dust.

[Experimental Example 3: Confirmation of Nrf2 expression effect of Gaetgang Liver Extract]

NF-E2 related factor 2 (Nrf2) is a major regulator that regulates a series of proteins called phase II enzymes that protect the human body from oxidative stress (fine dust, heat, etc.) and detoxify xenobiotics. have. In the absence of oxidative stress, Nrf2 in the cytoplasm is bound to the Keapl protein and is constantly ubiquitinated and decomposed, thereby inhibiting its activity. On the other hand, when there is oxidative stress, Nrf2 is released from the Keapl protein, moves into the nucleus, and binds to an antioxidant response element (ARE) present in the antioxidant gene promoter to express antioxidant enzymes to remove oxidative stress. do. Through these roles, Nrf2 is known to play an important role in the maintenance of cell homeostasis. In this experiment, we tried to confirm the effect of Nrf2 expression on Gaetgang extract.

Human-derived keratinocytes (HaCaT) cells were dispensed into 3×10 ⁵ cells/well in a 6 well plate, and the cells were stabilized for 1 day in a 37° C., 5% CO ₂ cell incubator. Cells were collected using a scraper after treating 3, 10, 30, and 100 µg/ml of the gland extract, and reacting for 6 hours. Protein was separated using 1x cell lysis buffer, and the protein was quantified by BCA assay, and a certain amount of protein was electrophoresed on a 10% SDS-PAGE gel. The isolated protein on the gel was transferred to the PVDF membrane and blocked for 1 hour using 5% skim milk. After washing 3 times with 1x TTBS solution and reacting the primary antibody (Nrf2, Cell signaling, USA) over night at 4° C., the secondary antibody bound with HRP was reacted for 2 hours at room temperature. Thereafter, washing was performed 3 times with 1x TTBS and the ECL solution was reacted to detect a band with a Chmi Doc instrument. The amount of Nrf2 expression was calculated by quantifying the expression of beta-actin, and quantified using Image J 1.47 software. The experimental results are shown in FIG. 3.

As shown in Figure 3, it was confirmed that the intestinal lumen extract of the present invention increases Nrf2 expression.

[Experimental Example 4: Confirmation of the inhibitory effect of MMP-1 expression on Gaetgang extracts]

In this experiment, we tried to confirm the effect of the Gaet River Liver Extract on MMP-1, whose expression is promoted by fine dust.

Human skin fibroblasts (HDFn) were dispensed into 6 well plates at a concentration of 4×10 ⁵ cells/well and stabilized in a 37° C., 5% CO ₂ incubator. After replacing with 2% FBS-added medium, fine dust (50ppm) and cannula extract 3, 10, 30, and 100 µg/ml were treated respectively, reacted for 6 hours, and then scraped. Was collected. Protein was separated using 1x cell lysis buffer, and the protein was quantified by BCA assay, and a certain amount of protein was electrophoresed on a 10% SDS-PAGE gel. The isolated protein on the gel was transferred to the PVDF membrane and blocked for 1 hour using 5% skim milk. After washing 3 times with 1x TTBS solution and reacting the primary antibody (MMP-1, Santacr, uz) over night at 4° C., the secondary antibody bound with HRP was reacted for 2 hours at room temperature. Thereafter, washing was performed 3 times with 1x TTBS and the ECL solution was reacted to detect a band with a Chmi Doc instrument. The amount of MMP-1 expression was calculated by quantifying based on the expression of beta-actin and quantified using Image J 1.47 software. As a positive control, 400 μM adenosine was used, and the experimental results are shown in FIG. 4.

As shown in Figure 4, it was confirmed that the Gaetgang extract of the present invention effectively suppresses the expression of MMP-1 caused by fine dust and has an effect of improving wrinkles.

[Experimental Example 5: Confirmation of NO inhibition rate of Gaet River Liver Extract]

In this experiment, we tried to confirm the effect of the Gaet River Liver Extract on NO (nitrogen oxide) produced by fine dust.

After dispensing RAW 264.7 cells, which are mouse-derived macrophages, into a 96 well plate at 1×10 ⁵ cells/well, cells were stabilized for 1 day in a 37° C., 5% CO ₂ cell incubator. After treating fine dust of 200µg/ml and 3, 10, 30, and 100 µg/ml of canine extracts, NDGA (nordihydroguaiaretic acid) was treated as a positive control at a concentration of 5µM. After reacting for 24 hours, 100 µl of the culture supernatant was separated, 100 µl of the same amount of Grease reagent was reacted at room temperature, and absorbance was measured at 540 nm using a microplate reader. The concentration of nitric oxide was calculated by comparison with a standard straight line obtained using sodium nitrite (NaNO ₂ ). The experimental results are shown in FIG. 5.

As shown in FIG. 5, it was confirmed that the gaet gang extract of the present invention effectively inhibits NO caused by fine dust and has antioxidant efficacy.

[Example 2: Preparation of cosmetic composition containing Gaetgang extracts]

A cosmetic composition containing a cane extract was prepared at a mixing ratio as shown in Table 2 below.

ingredient Example 2 (wt%) Comparative Example 1 (% by weight)
A Example 1 5.0 - EDTA-2Na 0.02 0.02 Purified water to 100 to 100




B
Cetostearyl alcohol 2.0 2.0 Glyceryl stearate 1.5 1.5 Microcrystal 0.7 0.7 Squalane 5.0 5.0 Floating paraffin 3.0 3.0 Trioctanoin 5.0 5.0 Polysorbate 1.2 1.2 Sorbitan stearate 0.5 0.5 Tocopheryl Acetate 0.2 0.2 Cyclomethicone 3.0 3.0 BHT 0.05 0.05 C Incense, preservative Proper Proper

[Experimental Example 6: Confirmation of wrinkle improvement effect of cosmetic composition containing Gaetgang extracts]

In this experiment, a human body application test was conducted on the effect of improving the skin wrinkles of the cosmetic composition containing the Gaetgang extract.

The experiment was conducted on 20 women aged 20 to 35 years, and the cosmetic composition (Example 2) containing the cannula extract was compared with the cosmetic composition (Comparative Example 1) containing no cannula extract on the right and left sides, respectively. The experiment was carried out by coating. Wrinkle improvement effect of each subject after 8 weeks of application by applying once a day was evaluated by visual observation and instrument measurement (cutometer SEm 575, C+K Electronic Co., Germany) of an experienced doctor, and the experimental results are shown in Table 3 below. It is shown in.

Wrinkle improvement effect Improvement rate (%) Great Some none Example 2 14 2 4 75.0 Comparative Example 1 0 11 9 27.5

As shown in Table 2 above, the cosmetic composition containing the Gaetgang Liver Extract of the present invention showed a high wrinkle improvement effect of 75.0%.

[Experimental Example 7: Confirmation of the expression level of inflammatory cytokines by fine dust in cosmetic compositions containing Gaetgang extracts]

In this experiment, we tried to confirm the effect of the cosmetic composition containing Gaetgang extract on inflammatory cytokines caused by fine dust.

The experimenter was conducted on 20 women aged 20 to 35 years, and a lipid absorbing tape (sebutape) was attached to the lower part of the skin before applying fine dust to obtain cytokines on the skin surface. After conducting 5 times, each was immersed in a glass vial containing 1 ml PBS buffer, sonicated for 1 hour, and then the cytokine content was measured.

After mixing the fine dust (500 ppm) 1:1 with mineral oil, treat it in the lower extremity and apply a cosmetic composition (Example 2) and a cosmetic composition that does not contain a gastrointestinal activity extract (Comparative Example 1). Each was coated with a lipid absorbing tape (sebutape) and removed after 24 hours to obtain cytokines in the same manner as above.

The cytokine content was quantified using the ELISA kit, the total protein amount was quantified using the protein quantification kit, and the result was analyzed by correcting the cytokine content to the total protein content. The experimental results are shown in FIG. 6.

As shown in Figure 6, it was confirmed that the cosmetic composition containing the cannula extract of the present invention significantly reduces the expression levels of inflammatory cytokines TNF-α and IL-8 caused by fine dust.

Claims (10)

delete delete Angelica japonica contains 70% (v/v) ethanol extract,
A cosmetic composition for strengthening skin barrier damaged by fine dust, characterized by increasing the expression of Claudin-1 and Desmoglein-2 reduced by fine dust.
delete delete delete Angelica japonica contains 70% (v/v) ethanol extract,
A cosmetic composition for improving skin wrinkles caused by fine dust, characterized by inhibiting the expression of MMP-1 caused by fine dust.
delete delete The method of claim 3 or 7,
70% (v/v) ethanol extract of the swelling,
Cosmetic composition comprising 0.001 to 30% by weight relative to the total weight of the cosmetic composition.

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