KR102525852B1 - Method of cosmetic composition comprising peptide growth factor using stem cell and cosmetic composition manufactured by the same and cosmetic comprising the cosmetic composition - Google Patents

Abstract

The present invention provides a method for preparing a cosmetic composition containing a growth factor peptide using stem cells, a cosmetic composition prepared thereby, and a cosmetic product including the cosmetic composition. The cosmetic composition manufacturing method includes the steps of preparing by freeze-drying at least two or more types of epidermal growth factors (EGF), including stem cell growth factors, in a state in which each receptor is supported, the top of the first chamber The opened part of is connected through a connector, the connector is disposed in a second chamber disposed on top of the first chamber, and disposing a collector so as to cover all of the upper end of the connector in the second chamber. , seating each of the freeze-dried receptors on a first tray disposed in a first chamber and connected to a rotatable first rotating body, and rotating the tray by rotating the first rotating body, the second A step in which an open portion of an upper portion of a chamber is connected through a ventilator, and air moves from the first chamber through the second chamber and through the ventilator by rotating a propeller disposed inside the ventilator, respectively. The two or more types of epidermal growth factors supported on the receptor of are separated from each other and collected in the aggregate, separating the aggregate, and lyophilizing the separated aggregate.

Description

Method for manufacturing a cosmetic composition containing growth factor peptides using stem cells, and a cosmetic composition prepared thereby and cosmetics containing the cosmetic composition THE COSMETIC COMPOSITION}

The present invention relates to a method for preparing a cosmetic composition containing a growth factor peptide using stem cells, a cosmetic composition prepared thereby, and a cosmetic product containing the cosmetic composition.

Cosmetics are products that clean the human body or beautify the user to stand out, and interest in cosmetics is increasing as the economy develops and consumption level increases. Accordingly, the trend is to select, process, and use raw materials with various functionalities while securing stability for the skin as well as raw materials for cosmetic compositions used in cosmetics. There is an increasing demand for raw materials capable of

On the other hand, stem cells promote skin tissue regeneration and provide many useful functions to the human body by complex proteins secreted from stem cells. Most of the complex proteins are known as growth factors, and active research on these growth factors is currently being conducted. In addition, various studies are being conducted to suppress the aging of the body by supplying these growth factors from the outside, and in addition, many attempts are being made to obtain wound healing, whitening, and antioxidant effects by growth factors through cosmetic compositions. It is becoming.

Therefore, in recent years, research and development to make it suitable for use as a cosmetic composition and cosmetics using the growth factor using stem cells is actively progressing.

Korean Patent Publication No. 10-2017-0005480 (published on July 21, 2017)

Accordingly, the present invention is to provide a method for preparing a cosmetic composition containing growth factor peptides using stem cells capable of promoting skin regeneration and providing various useful functions to the human body.

In addition, it is intended to provide a method for manufacturing a cosmetic composition that can stably function while being used as a cosmetic composition using growth factor peptides using stem cells.

In addition, it is intended to provide a cosmetic composition containing growth factor peptides using stem cells, which is prepared according to the above manufacturing method, can promote skin regeneration, and can provide various useful functions to the human body, and cosmetics containing the same. .

The object of the present invention is not limited to the above. The subject of the present invention will be understood from the entire contents of this specification, and those of ordinary skill in the art to which the present invention belongs will have no difficulty in understanding the additional subject of the present invention.

Method for manufacturing a cosmetic composition containing growth factor peptides using stem cells according to an embodiment of the present invention for solving the above problems is at least two kinds of epidermal growth factors (Epidermal Growth Factor: EGF) including stem cell growth factors ) Freeze-drying and preparing each of them in a state supported in each receptor, the open part of the top of the first chamber is connected through a connector, and the connector is placed in the second chamber disposed on the top of the first chamber. Arranged, arranging a collecting body to cover all of the upper end of the connector in the second chamber, each of the freeze-dried on a first tray disposed in the first chamber and connected to the rotatable first rotating body Rotating the tray by seating the receptors and rotating the first rotating body, connecting the open portion of the upper end of the second chamber through a ventilating tube, and rotating a propeller disposed inside the ventilating tube, allowing air to move from the first chamber through the second chamber through the ventilator, separating the two or more types of epidermal growth factors supported on each of the receptors and collecting them in the collection body; It includes the step of separating the collection body, and the step of lyophilizing the separated collection body.

In addition, the at least two epidermal growth factors are RH-polypeptide-11, RH-polypeptide-1, RH-polypeptide-9, RH-polypeptide-60, RH-polypeptide-64, RH-poly It may be characterized in that it is at least two kinds of epidermal growth factors composed of peptide-3, RH-polypeptide-22, RH-polypeptide-4, RH-oligopeptide-1 and RH-oligopeptide-2.

In addition, a step of preparing a third chamber having a dust collecting member connected to the lower end, a rotatable second rotating body inside, and a second tray connected to the second rotating body, and mixing connected to the lower portion of the dust collecting member. Preparing an apparatus equipped with a chamber, a raw material inlet line connected to one side of the mixing chamber, and a raw material outlet line connected to the other side of the mixing chamber, placing the lyophilized aggregate on the second tray and Rotating the second tray by rotating the rotating body, obtaining air from the mixing chamber to obtain two or more types of epidermal growth factors separated from the collection body, and supplying the first raw material through the raw material inlet line. It may be characterized by further comprising the step of injecting and mixing with the epidermal growth factor and discharging through the raw material outlet line.

In addition, the step of rotating the second tray may be characterized in that it is performed by applying ultrasonic waves or vibration to the collecting body.

In addition, the receptor or the collector may be characterized in that it is formed of a non-woven fabric material or a porous material composed of polypropylene.

In addition, the step of disposing the collector is characterized by disposing a filter on top of the collector, and disposing a porous auxiliary member having a plurality of holes formed on top of the filter, and separating the collector It may be characterized in that it is performed by removing the filter disposed on top of the collection body.

A cosmetic composition containing a growth factor peptide using stem cells according to an embodiment of the present invention for solving the above problems is prepared by the method for manufacturing a cosmetic composition of the present invention described above, and includes at least two types of epidermal growth factors ( Epidermal Growth Factor: EGF), wherein the epidermal growth factor is RH-polypeptide-11, RH-polypeptide-1, RH-polypeptide-9, RH-polypeptide-60, RH-polypeptide -64, RH-polypeptide-3, RH-polypeptide-22, RH-polypeptide-4, RH-oligopeptide-1 and RH-oligopeptide-2 composed of a mixture of at least two epidermal growth factors include

Cosmetics containing growth factor peptides using stem cells according to an embodiment of the present invention for solving the above problems are barberry root extract, coriander tree fruit extract, pasqueflower extract, Usnia extract, grapefruit seed extract, grapefruit seed extract, A cosmetic composition containing hairy root extract, white dandelion stem extract, angelica root extract, licorice root extract, apricot extract, safflower extract, at least two types of epidermal cell growth factors, purified water and other additives, The cosmetic composition containing at least one type of epidermal growth factor is characterized in that it is the cosmetic composition of the present invention described above.

In addition, the cosmetics may be characterized in that it further comprises a moisturizing agent, thickening agent.

In addition, the other additives may be characterized in that they include at least one of a preservative, a solubilizer, a pH adjusting agent, a flavoring agent, and a coloring agent.

Details of other embodiments are included in the detailed description and drawings.

According to the method for manufacturing a cosmetic composition containing growth factor peptides using stem cells of the present invention, a cosmetic composition containing growth factor peptides using stem cells that can promote skin regeneration and provide various useful functions to the human body can be manufactured.

In addition, a cosmetic composition capable of minimizing damage while mixing various types of epidermal growth factors, promoting skin regeneration, and providing various useful functions to the human body such as anti-inflammatory and anti-aging effects can be prepared. .

In addition, a cosmetic composition that can stably function while being used as a cosmetic composition can be prepared using growth factor peptides using stem cells.

In addition, the cosmetic composition prepared according to the above manufacturing method and cosmetics including the same can promote skin regeneration and provide various useful functions to the human body.

Effects according to the present invention are not limited by the contents exemplified above, and more various effects are included in the present specification.

1 is a diagram showing a process in which two or more types of epidermal growth factors are mixed according to an embodiment of the present invention.
Figure 2 is a view showing a collection body applied to the device of Figure 1 according to an embodiment of the present invention.
Figure 3 is a view showing a collection body applied to the device of Figure 1 according to another embodiment of the present invention.
Figure 4 is a view showing a process for using it as a cosmetic composition when two or more types of epidermal growth factors are collected in the collection body in one embodiment of the present invention.

Advantages and features of the present invention, and methods of achieving them, will become clear with reference to the detailed description of the following embodiments taken in conjunction with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, but will be implemented in various different forms, only these embodiments make the disclosure of the present invention complete, and common knowledge in the art to which the present invention belongs. It is provided to fully inform the holder of the scope of the invention, and the present invention is only defined by the scope of the claims. Like reference numbers designate like elements throughout the specification. The sizes and relative sizes of layers and regions in the drawings may be exaggerated for clarity of explanation.

The spatially relative terms "below", "beneath", "lower", "above", "upper", etc. are different from one element or component to another. It can be used to easily describe the correlation with elements or components. In addition, in the present specification, "one side" means a side spatially in one direction, and "the other side" may mean a side spatially opposite to the "one side", but they are also one element or component. It can be used to easily describe the correlation between and other elements or components.

Although first, second, etc. are used to describe various components, these components are not limited by these terms, of course. These terms are only used to distinguish one component from another. Accordingly, it goes without saying that the first component mentioned below may also be the second component within the technical spirit of the present invention.

Hereinafter, embodiments of the present invention will be described with reference to the drawings.

1 is a diagram showing a process in which two or more types of epidermal growth factors are mixed according to an embodiment of the present invention. In addition, FIG. 2 is a view showing a collection body applied to the device of FIG. 1 according to an embodiment of the present invention.

Referring to Figures 1 and 2, at least two or more types of epidermal growth factors (Epidermal Growth Factor: EGF), including stem cell growth factors, are loaded in respective receptors (100, 200, 300) and lyophilized, respectively. Preparing, the open part of the top of the first chamber 10 is connected through connectors 80 and 90, and the connectors 80 and 90 are connected to the second chamber 10 at the top of the first chamber 10 Disposed in the chamber 20, arranging the collection body 400 to cover all of the upper ends of the connectors 80 and 90 in the second chamber 20, disposed in the first chamber 10 Each of the freeze-dried receptors 100, 200, and 300 is seated on the first tray 70 connected to the rotatable first rotating body 50, and the first rotating body 50 is rotated. Rotating the tray 70, the open portion of the upper end of the second chamber 20 is connected through the ventilation tube 30, by rotating the propeller 40 disposed inside the ventilation tube 30, Allowing air to move from the first chamber 10 through the second chamber 20 through the ventilator 30, each of the two or more types of epithelium supported on the receptors 100, 200, and 300 Cell growth factors are separated and collected in the collection body 400, separating the collection body 400, and freeze-drying the separated collection body.

The growth factor peptide is epidermal growth factor (EGF), and at least two or more may be mixed. In addition, the at least two epidermal growth factors are RH-polypeptide-11, RH-polypeptide-1, RH-polypeptide-9, RH-polypeptide-60, RH-polypeptide-64, RH-poly It may be characterized in that it is at least two kinds of epidermal growth factors composed of peptide-3, RH-polypeptide-22, RH-polypeptide-4, RH-oligopeptide-1 and RH-oligopeptide-2.

Epidermal Growth Factor (EGF) can be properly supplied to damaged skin tissue so that skin tissue can be restored normally. Epidermal growth factor can be produced from microorganisms by using genetic engineering technology and protein separation and purification technology to produce epidermal growth factor similar to that of the human body. After producing, it is possible to mass-produce by gently isolating only the epidermal growth factor using protein separation and purification technology. The epidermal growth factor of the present invention may be derived from human stem cells.

Epidermal growth factor can promote the proliferation of epithelial cells and dermal cells of the skin, and promote cell growth of fibroblasts containing collagen, a component of the dermis. In addition, it can promote the synthesis of fibronectin, which is a substance that induces the secretion of angiogenesis promotion and other regeneration promoting factors in the skin damaged area, and forms a network while oriented in an orderly manner in the skin tissue. Therefore, it is possible to play a key role in skin regeneration, such as minimizing scarring at the wound site.

Epidermal growth factor (EGF) is RH-polypeptide-11, RH-polypeptide-1, RH-polypeptide-9, RH-polypeptide-60, RH-polypeptide-64, RH-polypeptide-3, It may consist of RH-polypeptide-22, RH-polypeptide-4, RH-oligopeptide-1 and RH-oligopeptide-2. As described above, two or more types of epidermal growth factors may be used in combination. Epidermal growth factor may not perform its function when one epidermal growth factor is used due to poor stability in light, heat, air, etc. When the epidermal growth factor does not perform its function, it is possible to prevent the deterioration of the skin regeneration effect by other epidermal growth factors.

In this way, when mixing different epidermal growth factors and using them as a cosmetic composition, in the conventional case, when preparing each epidermal growth factor and directly manufacturing it into a cosmetic composition, raw materials containing epidermal growth factors should be prepared and stored separately. In addition, in the process of manufacturing it as a final cosmetic product, each corresponding epidermal growth factor raw material had to be prepared, so a lot of storage equipment was required in the mass production process, a lot of space was required, and unnecessary labor was input. However, in the case of the present invention, by pre-mixing two or more different epidermal growth factors and freeze-drying, the pre-mixed raw materials can be stored in one storage place, making storage easy and mass production easy. In addition, as will be described later, cosmetics can be easily manufactured by mixing and impregnating the collector 400 in advance, or by mixing two or more types of epidermal growth factors with the collector 400 itself and freeze-drying them.

In addition, in the cosmetic composition manufacturing method of the present invention, each component is separated from each lyophilized body by centrifugal force by rotating the first rotating body 50 in a state in which each epidermal growth factor is prepared in a lyophilized state, and , By forcibly moving through the propeller 40 to mix and gather them into the collection body 400, it is possible to prevent loss of activity as much as possible by ensuring smooth mixing even if they do not completely melt each of them. Non-limitingly, the rotating step may be performed by applying ultrasonic waves or vibration to the receptors 100, 200, and 300. Accordingly, the raw materials located in the receptors 100, 200, and 300 can be more easily separated from the receptors 100, 200, and 300.

For example, in the conventional case, each epidermal growth factor is prepared by preparing freeze-dried raw materials, storing them separately, and then dissolving and mixing them when producing cosmetics, so a lot of storage space is required and productivity is low. However, in the case of the present invention, by mixing each prepared epidermal growth factor lyophilized product in a state that is not completely dissolved and freeze-drying again, it is possible to prepare a pre-mixed freeze-dried raw material while minimizing damage to the raw material.

On the other hand, the receptor (100, 200, 300) or the collection body 400 may be characterized in that formed of a non-woven material or a porous material composed of polypropylene. Accordingly, each component can be sufficiently accommodated (retained) in the space formed inside.

On the other hand, the first chamber 10 may have an opening and closing opening 11 so that the first chamber 10 is spatially separated from the outside when necessary while accommodating each raw material, and the second chamber 20 also collects when necessary. An opening and closing opening may be provided to allow the sieve 400 to be inserted or taken out. In addition, a separate control unit 15 may be included to drive the above components, and through the control unit 15, driving of each component may be turned on or off or driving conditions may be controlled.

The first chamber 10 has an open upper end, and may be connected to the second chamber 20 through the lower end 80 of the connector connected to the opened portion and the upper end 90 of the connector connected thereto. Except for the connectors 80 and 90, the first chamber 10 and the second chamber 20 may be spatially separated from each other, and accordingly, the lyophilized raw materials in the first chamber 10 are separated and When heading to the second chamber 20, it can be moved only through the connectors 80 and 90. Accordingly, each of the raw materials can be uniformly mixed and collected in the collector 400 formed while covering the upper end 90 of the connector. Although not shown separately, an opening/closing device (not shown) may be provided at the lower end 80 or the upper end 90 of the connector, and a separate ventilation facility (not shown) is provided inside the first chamber 10 to provide a first After the raw materials are sufficiently mixed in the chamber 10, the opening and closing device (not shown) may be opened so that the raw materials mixed with the collecting body 400 are mixed and collected.

In addition, the collection body 400 is arranged to cover all the open portions of the top end 90 of the connector in the second chamber 20, thereby preventing raw materials from leaking out of the collection body 400. can

The first tray 70 is connected through the first rotating body leg 60 connected to the rotatable first rotating body 50 and can rotate together when the first rotating body 50 rotates, and a plurality of raw materials A plurality of them may be spaced apart so that the receptors 100, 200, and 300 carrying them may be disposed, respectively. Rotating the tray may be performed by seating each of the lyophilized receptors 100, 200, and 300 on a first tray 70 and rotating the first rotating body. Each epidermal growth factor is separated from the receptors 100, 200, and 300 according to the centrifugal force, and as the propeller 40 formed inside the ventilation pipe 30 rotates, air is moved and mixed into the collector 400. and can be captured.

The ventilation tube 30 is connected to the opening formed at the upper end of the second chamber 30, and the first chamber 10, the second chamber 20, and the ventilation tube 30 are connected by the propeller 40 located inside. In the formed space, the moving direction of the air can be moved from the first chamber 10 to the second chamber 20 and from the second chamber 20 to the ventilation pipe 30. Accordingly, each of the raw materials sufficiently mixed in the first chamber 10 can be collected in the collector 400 . As already described above, a separate opening/closing device (not shown) may be provided between the first chamber 10 and the second chamber 20, and a separate ventilation facility (not shown) may be provided in the first chamber 10 itself. When) is provided and after mixing is sufficiently performed in the first chamber 10 itself, an opening/closing device (not shown) may be opened to be collected by the collecting body 400 .

On the other hand, by rotating the tray and moving the air, the two or more types of epidermal growth factors supported on each of the receptors 100, 200, and 300 are separated and collected in the collection body 400. (collected) can be. Thereafter, when the collection is sufficiently performed, the collection body 400 is separated, and the separated collection body 400 is lyophilized to a state in which the raw materials of each epidermal growth factor are not completely dissolved. They can also be sufficiently mixed and lyophilized and stored.

On the other hand, Figure 3 is a view showing the collection body applied to the device of Figure 1 according to another embodiment of the present invention is shown.

Referring to FIG. 3, in the step of disposing the collector, a filter 421 is placed on top of the collector 401, and a porous auxiliary member 411 having a plurality of holes is formed at the top of the filter 421. It is characterized by disposing, and the step of separating the collecting body 401 may be characterized in that it is performed by removing the filter 421 disposed on top of the collecting body 401. The porous auxiliary member 411 is formed with a plurality of holes so that air can easily move, and the filter 421 can prevent the movement of epidermal growth factors as much as possible while allowing a certain amount of air to pass through. Thereafter, by easily removing the filter 421 , higher purity mixed epidermal growth factors may be accommodated in the collecting body 401 .

On the other hand, Figure 4 is a view showing a process for using it as a cosmetic composition when two or more types of epidermal growth factors are collected in the collection body in one embodiment of the present invention.

Referring to FIG. 4, a dust collecting member 600 is connected to the lower end, a rotatable second rotating body 1000 inside, and a second tray 1100 connected to the second rotating body 1000 are provided. Preparing the three chambers 500, the mixing chamber 800 connected to the lower part of the dust collecting member 600, the raw material inlet line 910 connected to one side of the mixing chamber 800, and the mixing chamber 800 Preparing a device having a raw material outlet line 920 connected to the other side of the lyophilized collection body 400 is seated on the second tray 1100 and the second rotating body 1000 is rotated rotating the second tray 1100, inhaling air in the mixing chamber 800 to obtain two or more types of epidermal growth factors separated from the collection body 400, and the raw material inlet line Injecting the first raw material through (910), mixing with the epidermal growth factor, and discharging the first raw material through the raw material outlet line (920) may be further included.

An empty space may be formed at the lower end of the third chamber 500 so that the mixed epidermal growth factor raw material can be directed to the dust collecting member 600 formed at the lower part, and the mixing chamber 800 passes through the lower part 700 of the dust collecting member. can be connected with Without limitation, the dust collecting member 600 may have a shape in which an area becomes narrower toward the lower end. In addition, although not shown separately, a separate fixing member (not shown) may be installed to fix the collector 400 on the second tray 1100 so as not to be separated when the collector 400 rotates. It may be, the collecting body 400 may be fixed or separated by the fixing member (not shown).

A raw material inlet line 910 is connected to one side of the mixing chamber 800 and a raw material outlet line 920 may be provided to the other side. Other cosmetic compositions, purified water, and other additives may be introduced through the raw material inlet line 910, and they are mixed with two or more types of epidermal growth factors introduced into the mixing chamber 800 through the dust collecting member 600. It can be discharged through the raw material outlet line 920. Although not shown separately, but not limited to, between the mixing chamber 800 and the raw material inlet line 910, between the mixing chamber 800 and the raw material outlet line 920, and between the lower part of the dust collecting member 700 and the mixing chamber 800 ) Between them, an opening and closing opening (not shown) may be disposed as needed.

Meanwhile, the step of rotating the second tray 1100 may be performed by applying ultrasonic waves or vibration to the collector 400 . Accordingly, it is possible to more easily separate the raw materials from the collecting body 400 .

Through the above process, the two or more kinds of epidermal growth factor raw materials present in the lyophilized state inside the collection body 400 are separated from the collection body 400, and the materials of other cosmetic compositions are separated through the mixing chamber 800. By mixing, mass production can be facilitated.

On the other hand, the present invention provides a cosmetic composition, and the cosmetic composition according to an embodiment of the present invention is prepared by the method for preparing the cosmetic composition of the present invention described above, and includes at least two types of Epidermal Growth Factor (EGF). ), wherein the epidermal growth factor is RH-polypeptide-11, RH-polypeptide-1, RH-polypeptide-9, RH-polypeptide-60, RH-polypeptide-64, RH- It includes a mixture of at least two or more epidermal growth factors composed of polypeptide-3, RH-polypeptide-22, RH-polypeptide-4, RH-oligopeptide-1 and RH-oligopeptide-2.

The epidermal growth factor (EGF) is 0.001 to 0.1% by weight of RH-polypeptide-11, 0.001 to 0.1% by weight of RH-polypeptide-1, and 0.001 to 0.1% by weight of RH-polypeptide-9 in the cosmetic composition %, 0.001 to 0.1% by weight of RH-polypeptide-60, 0.001 to 0.1% by weight of RH-polypeptide-64, 0.001 to 0.1% by weight of RH-polypeptide-3, 0.001 to 0.1% by weight of RH-polypeptide-22 0.1% by weight, 0.001 to 0.1% by weight of RH-polypeptide-4, 0.001 to 0.1% by weight of RH-oligopeptide-1 and 0.001 to 0.1% by weight of RH-oligopeptide-2, in addition to purified water and other additives. In the above range, it is possible to increase the miscibility with other epithelial cell growth factors, and to achieve a skin regeneration effect.

On the other hand, the present invention provides a cosmetic, and the cosmetic according to an embodiment of the present invention is barberry root extract, coriander tree fruit extract, pasqueflower extract, Usnia extract, grapefruit seed extract, grapefruit seed extract, hair root extract, white dandelion Stem extract, angelica root extract, licorice root extract, apricot extract, safflower extract, a cosmetic composition containing at least two epidermal growth factors, purified water and other additives, including at least two epidermal growth factors The cosmetic composition comprising a is characterized in that the cosmetic composition of the present invention described above.

The barberry root extract contains medicinal ingredients such as berberine, coptisin, warrenin, palmatin, etc., and can produce effects such as anti-acne effect, skin soothing effect, antibacterial effect, and anti-inflammatory effect. In addition, the fruit extract of the corium chinensis can act as a preservative in cosmetic compositions due to its excellent antibacterial and anti-inflammatory action. The pasqueflower extract, along with the extract of the fruit of Pascal sinensis, can act as a preservative in the cosmetic composition, can provide a skin soothing effect, and contains a large amount of saponin to keep the skin healthy and smooth. In addition, pasqueflower extract has antipyretic, astringent, anti-inflammatory, and bactericidal effects, so it can produce useful effects such as neuralgia, bleeding, and dysmenorrhea. The Usnia extract is rich in flavonoids, so it can provide a skin conditioning effect, an effect of imparting vitality and vitality to the skin, a skin protection effect, and a skin moisturizing effect. In addition, the grapefruit seed extract is rich in vitamin C and vitamin E, so it can produce strong antibacterial, antiviral, antiparasitic, antibacterial, and antioxidant effects.

In addition, the hair root extract contains a large amount of saponin, so it can enhance skin elasticity, supply nutrients, soothe skin, improve skin tone, moisturize, discharge toxins, and produce antibacterial substances. In addition, the white dandelion stem extract is rich in vitamin C, vitamin D, vitamin A, and vitamin B complex, and contains iron, calcium, and zinc, which can improve kidney function and liver function, and is active as an antioxidant. Aging can be suppressed by removing oxygen. In addition, the angelica gigas root extract is effective in preventing atopy and inflammation due to its excellent antibacterial action, and can protect the skin from active oxygen by means of a component called decorcine. In addition, the licorice root extract contains a glabridin component, so it can produce excellent effects on anti-inflammatory and whitening effects, and can be involved in improving skin immunity to soothe the skin. In addition, the apricot extract is rich in protein, fat, iron, vitamins, etc., so that it can have skin moisturizing effect, skin aging prevention effect, and skin waste removal effect. In addition, the safflower extract may have a skin conditioning effect.

On the other hand, the cosmetic of the present invention contains 0.1 to 5% by weight of the barberry root extract, 0.1 to 5% by weight of the coriander tree fruit extract, 0.1 to 5% by weight of pasqueflower extract, 0.1 to 5% by weight of usnia extract, grapefruit 0.1 to 5% by weight of seed extract, 0.1 to 5% by weight of grapefruit seed extract, 0.1 to 5% by weight of hairy root extract, 0.1 to 5% by weight of white dandelion stem extract, 0.1 to 5% by weight of Angelica gigas root extract , 0.1 to 5% by weight of licorice root extract, 0.1 to 5% by weight of apricot extract, 0.1 to 5% by weight of safflower extract, 0.01 to 2% by weight of epidermal growth factor (EGF), 30 to 80% by weight of purified water and other additives. While improving miscibility with other components within the above content range, it is possible to prevent deterioration in useful efficacy of each component.

Meanwhile, the cosmetics may further include a moisturizer and a thickening agent. The moisturizing agent may include glycerin, butylene glycol, propylene glycol, methylpropanediol, xylitol, betaine, or oligopolysaccharide, but is not limited thereto. The thickener is intended to impart some viscosity to cosmetics, and includes xanthan gum, hydroxyethyl cellulose, carbomer, acrylate/C10-30 alkyl acrylate crosspolymer, sodium polyacrylate or acrylate copolymer. It can be done, but is not limited thereto.

In addition, the other additives may include at least one of a preservative, a solubilizer, a pH adjusting agent, a flavoring agent, and a coloring agent.

The preservative may include methylparaben, propylparaben, phenoxyethanol, sodium benzoate, potassium sorbate, or chlorophenesin, and may prevent cosmetic ingredients from decomposing. In addition, the solubilizing agent may be used to dissolve components containing fat components in an aqueous solution during the manufacturing process, and polysorbate 20, polysorbate 60, PEG-40 hydrogenated castor oil or PEG-6 caprylic/capric glycerides. The pH adjusting agent may be for adjusting the pH of cosmetics to a level that is not harmful to the human body, and may include citric acid, lactic acid, glycolic acid or sodium citrate. The fragrance and coloring agent is for a user's olfactory or visual effect, and may include fragrance, essential oil, organic pigment, etc., but is not limited thereto.

As described above, the cosmetic composition containing the growth factor peptide using stem cells of the present invention and the cosmetic product containing the same provide various useful effects to the skin of the human body and contain the components of the growth factor peptide in a complex manner. While promoting regeneration, performing functions such as anti-inflammatory, anti-aging, and skin soothing effects, it is possible to minimize the deterioration of the effect of promoting skin regeneration by growth factor peptides.

Although the embodiments of the present invention have been described with reference to the accompanying drawings, the present invention is not limited to the above embodiments and can be manufactured in a variety of different forms, and those skilled in the art in the art to which the present invention belongs A person will understand that the present invention may be embodied in other specific forms without changing the technical spirit or essential features. Therefore, the embodiments described above should be understood as illustrative in all respects and not limiting.

10: first chamber
11: opening and closing opening of the first chamber
15: control panel
20: second chamber
21: opening and closing opening of the second chamber
30: ventilation
40: propeller
50: 1st rotating body
60: 1st rotating body leg
70: first tray
80: lower connector
90: top of connector
100: first receptor
200: second receptor
300: third receptor
400, 401: collection body
411: porous auxiliary member
421: filter
500: third chamber
600: dust collecting member
700: lower part of the dust collecting member
800: mixing chamber
910: raw material inlet line
920: raw material outlet line
1000: 2nd rotating body
1100: second tray

Claims (10)

Preparing at least two or more types of epidermal growth factors (Epidermal Growth Factor: EGF), including stem cell growth factors, by freeze-drying, respectively, in a state supported in respective receptors;
The open part of the upper end of the first chamber is connected through a connector, and the connector is disposed in a second chamber disposed on the upper end of the first chamber, and collected so as to cover all of the upper end of the connector in the second chamber. placing a sieve;
seating each of the freeze-dried receptors on a first tray disposed in the first chamber and connected to a rotatable first rotating body, and rotating the tray by rotating the first rotating body;
The open part of the upper part of the second chamber is connected through a ventilator, and by rotating a propeller disposed inside the ventilator, air moves from the first chamber through the second chamber through the ventilator step;
Separating the two or more types of epidermal growth factors supported on each of the receptors and collecting them in the collection body;
Separating the collection body; and
Including; lyophilizing the separated collection body;
The at least two epidermal growth factors are RH-polypeptide-11, RH-polypeptide-1, RH-polypeptide-9, RH-polypeptide-60, RH-polypeptide-64, RH-polypeptide- 3, RH-polypeptide-22, RH-polypeptide-4, RH-oligopeptide-1 and RH-oligopeptide-2 composed of at least two types of epidermal growth factors cosmetic composition manufacturing method, characterized in that. delete According to claim 1,
preparing a third chamber having a dust collecting member connected to a lower end, a second rotatable body inside, and a second tray connected to the second rotating body;
Preparing a device equipped with a mixing chamber connected to the lower part of the dust collecting member, a raw material inlet line connected to one side of the mixing chamber, and a raw material outlet line connected to the other side of the mixing chamber;
Rotating the second tray by seating the lyophilized collection body on the second tray and rotating the second rotating body;
obtaining two or more types of epidermal growth factors separated from the aggregate by sucking air from the mixing chamber; and
Injecting the first raw material through the raw material inlet line, mixing with the epidermal growth factor, and discharging the first raw material through the raw material outlet line; Cosmetic composition manufacturing method characterized in that it further comprises. According to claim 3,
Rotating the second tray is a cosmetic composition manufacturing method, characterized in that carried out by applying ultrasonic waves or vibration to the collection body. According to claim 1,
The method for producing a cosmetic composition, characterized in that the receptor or the collecting body is formed of a non-woven fabric material or a porous material composed of polypropylene. According to claim 1,
The step of disposing the collection body is characterized by disposing a filter on top of the collection body, and disposing a porous auxiliary member having a plurality of holes formed on top of the filter,
Separating the collector is a method for producing a cosmetic composition, characterized in that carried out by removing the filter disposed on top of the collector. delete delete delete delete

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