KR102588616B1 - Fixed temperature cell transfer device of without energy supply - Google Patents

Abstract

The present invention relates to a non-power constant temperature cell transfer device, which is equipped with a heating unit that introduces atmospheric oxygen and releases heat through a redox reaction of metal so as to continuously supply the optimal culture temperature to the culture vessel even without power supply. By storing the first container containing living animal cells and culture medium in the second container, an optimal culture environment for cell proliferation of the cells contained in the first container is maintained, thereby maintaining the activity and viability of the cells.

Description

Fixed temperature cell transfer device of without energy supply}

The present invention relates to a non-powered constant temperature cell transfer device, and more specifically, a non-powered constant temperature cell transfer device that supplies an optimal culture temperature even without power supply and transports cells while maintaining the horizontal state of the container storing cells even when subjected to external shock. It's about.

Generally, cell culture includes monolayer culture (adherent culture), in which cells proliferate by attaching to a culture medium, and suspension culture, in which cells proliferate in suspension.

Cells cultured using the above culture method react sensitively to the culture environment. The culture environment of cells has a strong correlation with cell proliferation and survival. In this way, in order to safely transport cells that react sensitively to the culture environment, a culture environment that allows cells to proliferate and survive must be maintained.

For general cell culture, continuous supply of carbon dioxide and maintenance of constant temperature and humidity are essential. The most common method of transporting existing cells is to place the cell container in liquid nitrogen and move it at a low temperature.

As seen in Republic of Korea Patent Publication No. 10-2015-0007636, the prior art that performs the above function includes a transporter enclosure having the same storage space, a cooling unit that maintains the internal temperature of the transporter enclosure at a constant level, and a cooling process of the cooling unit. In a cell transporter consisting of a heat dissipation unit that dissipates heat generated in the transporter enclosure to the outside of the transporter enclosure, a transport control unit that controls the operation of the cooling unit and the heat dissipation unit, and a charging power supply unit; On one side of the transporter enclosure, there is a transfer temperature manager that sets the temperature of the transfer process, displays it, and records the temperature history, a notification device that notifies the transporter when an abnormal situation occurs during the cell transfer process, and a GPS that allows the location of the transporter enclosure to be confirmed. It consists of a GPS receiver that receives signals and a wireless network module for access to the Internet and wireless networks.

However, the conventional technical configuration had the following problems.

There is a problem in that cooling the cells reduces their activity and viability during transport.

Additionally, there is a problem that power must be supplied to the device for maintaining the storage temperature of cells.

In addition, there is a problem in that the level of the container storing the cells is not maintained.

Republic of Korea Patent Publication No. 10-2015-0007636. (January 21, 2015)

The purpose of the present invention is to solve the above-described conventional problems and to continuously supply the optimal culture temperature to the culture vessel even without power supply.

In addition, another object of the present invention is to maintain the optimal culture temperature even without power supply.

In addition, another object of the present invention is to allow the container storing cells to be transported while maintaining its horizontal state even when exposed to external shock.

In order to solve this problem, the present invention accommodates a first container containing living animal cells and culture medium in a second container equipped with a heating unit that introduces atmospheric oxygen and releases heat through a redox reaction of the metal, The basic feature of its technical composition is that it allows the first container to be transported while maintaining the physiological activity temperature of the cells contained in the first container.

In order to solve this problem, the present invention maintains the horizontal state of the first container containing living animal cells and culture medium in a second container equipped with a heating unit that introduces atmospheric oxygen and releases heat through a redox reaction of the metal. A third container containing as much storage as possible is installed to enable transfer while maintaining the physiological activity temperature of the cells contained in the first container through the second container, and to minimize tilt of the first container through the third container. It is a basic feature of its technical composition.

In order to solve this problem, the present invention maintains the horizontal state of the first container containing living animal cells and culture medium in a second container equipped with a heating unit that introduces atmospheric oxygen and releases heat through a redox reaction of the metal. A third container containing as much storage as possible is installed to enable transfer while maintaining the physiological activity temperature of the cells contained in the first container through the second container, and to minimize tilt of the first container through the third container. A basic feature of its technical structure is that the third container is filled with carbon dioxide at a higher concentration than the carbon dioxide concentration in the atmosphere.

According to the present invention, the first container provides a space that can selectively determine the closed state, and allows culture medium to be injected, cell inoculated, removed, and obtained into the space while the space is closed, and It is characterized by enabling cell culture in an open space.

According to the present invention, the first container includes a closed passage for bringing in and out fluids, gases, and cells from the outside into and out of the space, a circulation filter for circulating gas necessary for cell culture in the space, and It is characterized by being composed of a scraper that is installed and moves to scrape cells to remove them from the bottom of the space.

According to the present invention, the first container is characterized in that the carbon dioxide concentration is 1% to 30%.

According to the present invention, the third container is characterized in that the carbon dioxide concentration is 1% to 30%.

According to the present invention, the temperature inside the first container is maintained at 22°C to 43°C through the heating unit.

According to the present invention, the heat generating unit is characterized in that it generates heat at 40°C to 60°C through a redox reaction between oxygen and metal in the atmosphere introduced into the second container.

According to the present invention, the heating part is characterized in that a thermal insulation material is mixed with metal powder that reacts with oxygen and generates heat.

According to the present invention, the second container further includes an opening and closing part that determines the inflow of atmospheric oxygen.

According to the present invention, the third container includes an inner container that has a spherical shape and has a heavy object installed on the inner bottom and a first container is seated on top of the heavy object, and an outer container that has a spherical shape and accommodates the inner container, and It is characterized by consisting of a plurality of wheels installed to roll and rotate between the inner container and the outer container.

According to the present invention, it further includes a fourth container for sealing the first container.

According to the present invention, the insulation material is characterized by selectively mixing one or more from the group consisting of sawdust, salt, and moisture.

According to the present invention, the opening and closing part includes an inlet hole that penetrates one side of the second container and allows atmospheric oxygen to flow into the second container, a stopper that selectively opens and closes the inlet hole, and is installed in the stopper to allow oxygen in the atmosphere to flow into the second container. It is characterized by consisting of an opening and closing member that blocks the inflow of oxygen when the internal temperature of the container rises.

According to the present invention, the outer container and the inner container are formed by assembling a hemispherical first auxiliary container and a second auxiliary container to form a sphere, and packing is provided between the first auxiliary container and the second auxiliary container. .

According to the present invention, the heavy object is characterized in that it is made of fluid.

According to the present invention, the heavy object is characterized by being composed of a solid having its own weight and a fluid filled on top of the solid.

According to the present invention, the opening and closing member is characterized by being applied as a bimetal or temperature-responsive linear material.

According to the present invention, the opening and closing member is characterized by being applied as a cylinder or piston.

As described above, the power-free constant temperature cell transfer device according to an embodiment of the present invention introduces oxygen from the atmosphere to continuously supply the optimal culture temperature to the culture vessel even without power supply through a redox reaction of the metal. The first container containing live animal cells and culture medium is stored in a second container equipped with a heating element that emits heat, and the optimal culture environment for cell proliferation of the cells contained in the first container is maintained to maintain cell activity and viability. It has an effect.

In addition, the power-free constant temperature cell transfer device of the present invention further includes an opening and closing part in the second container that determines the inflow of atmospheric oxygen so that the optimal culture temperature is maintained even without power supply, so that when the temperature of the container rises, oxygen is automatically supplied. Lowering the temperature by blocking the inflow and lowering it to the set temperature has the effect of maintaining the optimal temperature for cell culture by allowing oxygen to flow in.

In addition, the non-powered constant temperature cell transfer device of the present invention includes an inner container installed with a heavy object in a second container, an outer container accommodating the inner container, and By installing a third container consisting of a plurality of wheels installed to roll and rotate between the container and the external container, the effect of maintaining the horizontal state of the first container containing live animal cells and culture medium even if the bag shakes or tilts during transport There is.

Figure 1 is a cross-sectional view showing the power-free constant temperature cell transfer device of the present invention.
Figure 2 is an exploded perspective view showing the third container of the non-powered constant temperature cell transfer device of the present invention.
Figure 3 is a perspective view showing the first container of the power-free constant temperature cell transfer device of the present invention.
Figure 4 is a cross-sectional view showing the first container of the non-powered constant temperature cell transfer device of the present invention.
Figure 5 is a cross-sectional view showing the use state of the non-powered constant temperature cell transfer device of the present invention.

Hereinafter, the present invention will be described in detail with reference to the drawings attached to the present invention. First of all, it should be noted that among the drawings, identical components or parts are given the same reference numerals as much as possible. In describing the present invention, detailed descriptions of related well-known functions or configurations are omitted in order to not obscure the gist of the present invention.

Figure 1 is a cross-sectional view showing the unpowered constant temperature cell transfer device of the present invention, Figure 2 is an exploded perspective view showing the third container of the unpowered constant temperature cell transfer device of the present invention, and Figure 3 is a first container of the unpowered constant temperature cell transfer device of the present invention. It is a perspective view, Figure 4 is a cross-sectional view showing the first container of the non-powered constant temperature cell transfer device of the present invention, and Figure 5 is a cross-sectional view showing the use state of the unpowered constant temperature cell transfer device of the present invention.

First, as shown in FIG. 1, looking at the configuration of the non-powered constant temperature cell transfer device 10 according to the present invention, a second heating unit 210 is provided with a heating unit 210 that introduces atmospheric oxygen and releases heat through a redox reaction of the metal. The first container 100 containing live animal cells 11 and culture medium 12 is stored in the container 200, so that the first container 100 can be transported while maintaining the physiological activity temperature of the cells 11 contained in the first container 100.

In addition, according to another aspect of the present invention, the first container containing living animal cells 11 and culture medium 12 is placed in a second container 200 equipped with a heating part 210 that introduces atmospheric oxygen and releases heat through a redox reaction of the metal. A third container 300 is installed so that the horizontal state of the container 100 is maintained, so that the cells 11 contained in the first container 100 can be transferred through the second container 200 while maintaining the physiological activity temperature, and the third container 300 A non-power constant temperature maintenance cell transfer device 10 is provided, characterized in that the tilt of the first container 100 is minimized.

In addition, according to another aspect of the present invention, the first container containing living animal cells 11 and culture medium 12 is placed in a second container 200 equipped with a heating part 210 that introduces atmospheric oxygen and releases heat through a redox reaction of the metal. A third container 300 is installed so that the horizontal state of the container 100 is maintained, so that the cells 11 contained in the first container 100 can be transferred through the second container 200 while maintaining the physiological activity temperature, and the third container 300 An unpowered constant temperature maintenance cell transfer device 10 is provided, characterized in that the tilt of the first container 100 is minimized and the third container 300 is filled with carbon dioxide at a higher concentration than the carbon dioxide concentration in the atmosphere.

A more detailed look at the non-powered constant temperature cell transfer device 10 according to the present invention is as follows.

Referring to Figure 1, the power-free constant temperature cell transfer device 10 according to a preferred embodiment of the present invention consists of a first container 100 and a second container 200.

At this time, the first container 100 contains live animal cells 11 and culture medium 12, as shown in FIGS. 3 and 4.

The culture vessel 100 has a sealed space 101 inside.

The culture vessel 100 is made of a soft plastic material, and the size of the space 101 can be changed by external pressure or force.

The culture vessel 100 is provided with a space that can selectively determine the sealed state, and allows culture medium to be injected, cell inoculated, detached, and obtained into the space 101 while the space 101 is closed. , to enable culturing of cells in an open state.

In addition, in the process of obtaining cells 11, cells 11 are obtained excluding a portion of the total cultured cells 11, thereby enabling repeated cultivation of cells 11 remaining inside the culture vessel 100.

In addition, in the process of obtaining cells 11, all cultured cells are obtained, and then the culture medium is injected into the culture vessel 100, cells are inoculated, cultured, detached, and harvested can be repeatedly performed.

The culture vessel 100 is equipped with a closed passage 110 and a circulation filter 120 to enable continuous cultivation of the cells 11 as described above.

First, the closed passage 110 is installed in the culture vessel 100 to enable the process of injecting the culture medium 12 into the space 101, inoculating the cells 11 into the culture medium 12, and obtaining the cells 11. Even in the progress of the above process, the sealed container is sealed. The status is maintained.

That is, the sealed passage 110 is installed on the side of the culture vessel 100 to allow fluid, gas, and cells 11 to be brought in and out of the space 101 from the outside.

To enable this, the sealed passage 110 is installed on the surface of the culture vessel 100, and the sealed passage 110 is made of a soft block and installed in the space 101.

At this time, the process of injecting the culture medium 12 and cells 11 through the sealed passage 110 and obtaining cells 11 to the outside usually uses a syringe.

In other words, the sealed passage 110 is penetrated through the needle of the syringe, and then the culture medium 12 filled inside the syringe is injected into the space 101, or the cells 11 detached after culturing inside the culture vessel 100 are injected with a syringe using negative pressure of the syringe. It is sucked in and made available for external use.

Also, when the needle is removed from the sealed passage 110 after the injection of the culture medium 12 or the acquisition of the cells 11, the sealed passage 110 itself is sealed due to the elasticity of the sealed passage 110, thereby maintaining the airtightness of the space 101.

Additionally, the circulation filter 120 is installed to inject gas necessary for culturing cells 11 into the space 101 of the culture vessel 100.

That is, the culture vessel 100 is stored in the culture environment means 200 when cultivating the cells 11 and is provided with an appropriate temperature and at the same time is provided with the gas necessary for culturing the cells 11.

At this time, the culture vessel 100 allows gas to enter and exit the space 101 through the circulation filter 120.

In other words, the inflow of gas into the space 101 of the culture vessel 100 generates negative pressure inside the culture environment means 200 to change the size of the space 101 of the culture vessel 100, thereby cultivating cells 11 into the culture environment means 200 through the circulation filter 120. Ensure that the necessary gases can enter and exit space 101.

Here, the gas includes one or more of carbon dioxide and oxygen.

The specific configuration of the circulation filter 120 is as follows.

The circulation filter 120 consists of a pipe 121 installed on the side of the culture vessel 100, a valve 122 installed at the end of the pipe 121, and a filter 123 installed inside the valve 122.

Additionally, a clip 124 is mounted on the conduit 121 to selectively close and open the conduit 121.

The clip 124 opens the conduit 121 when storing the culture vessel 100 in the culture environment means 200 to allow gas to enter and exit the space 101. When the culture vessel 100 is to be taken out from the culture environment means 200, the clip 124 is used. The pipe 121 is interrupted to seal the space 101 of the sealed container.

In addition, since the cells 11 cultured on the bottom of the space 101 of the culture vessel 100 are not easily detached due to adhesion, a scraper 130, a separate tool for detaching the cells 11, is installed inside the space 101.

That is, the scraper 130 rotates or moves within the space 101 through mechanical external force, magnetic force, potential energy, etc. to scrape the cells 11 and remove the cells 11 from the bottom surface of the space 101.

At this time, the method for detaching cells 11 through magnetic force is as follows.

A separately provided moving member 140 is brought close to the outer lower surface of the culture vessel 100, and is magnetically connected to the scraper 130, so that the scraper 130 is interlocked within the space 101 by the movement of the moving member 140, so that the scraper 130 rubs and destroys the cells 11. Scrape and remove.

That is, the metal body 134 or the magnetic body 141 may be installed in the scraper 130 and the moving member 140, respectively, so that they can be integrated with each other by magnetic force.

In the cell 11 detachment method using potential energy, the culture vessel 100 is positioned at an angle so that the scraper 130, which has its own weight, slides on the bottom of the space 101 to scrape off the cells 11 and detach them.

The scraper 130 configured in this way forms a culture groove 132 at the top to enable filling of the culture medium 12 and culturing of cells 11.

In particular, the scraper 130 is configured as a rectangular culture vessel, but there is no limit to the shape of the culture vessel, such as a polygon such as a circle or triangle, as long as it has a surface to which cells can attach.

In addition, the scraper 130 has a lower surface that contacts the bottom surface of the space 101 and forms a plurality of blades 131.

Here, the scraper 130 has a lower surface in contact with the bottom surface of the space 101 forming a plurality of blades 131, and the blades 131 are formed to have a corner angle to separate the cells 11 from the bottom surface by friction with the bottom surface of the space 101. Let them do it.

Alternatively, the scraper 130 has a lower surface in contact with the bottom surface of the space 101 forming a plurality of blades 131, and the blades 131 are formed with continuous corner angles so that they are in contact or not in contact with the bottom surface of the space 101.

In addition, the scraper is made of polyethylene (PE), polypropylene (PP), polyamide (PA), polyacetel (POM), polyvinyl chloride (PVC), polyester (PET), polymethylpentene (PMP), and ionomer. (IO), ethylene vinyl alcohol (EVOH), polyvinyl chloride (PVA), polystyrene (PS), methacrylic resin (PMMA), polycarbonate (PC), vinyl acetate (PVAc), polyvinyl alcohol (PVA), Materials selected from phenol resin (PF), urea resin (UF), melamine resin (MF), epoxy resin (EP), polyurethane (PUR), unsaturated polyester resin (UP), and metal are applied.

Meanwhile, the first container 100 is stored inside the second container 200. The interior of the second container 200 is provided with a heating unit 210 that introduces atmospheric oxygen and releases heat through a redox reaction of the metal. That is, the first container 100 can be transported while maintaining the physiological activity temperature of the cells 11 contained in the first container 100.

Furthermore, the heating unit 210 generates heat at 40°C to 60°C through a redox reaction between oxygen and metal in the air introduced into the second container 200. At this time, in the heating unit 210, an insulating material is mixed with metal powder that reacts with oxygen and generates heat. Here, the insulating material is selectively mixed with one or more from the group consisting of sawdust, salt, and moisture.

That is, the temperature inside the first container 100 is maintained at 22°C to 43°C through the heating unit 210.

In the end, the power-free constant temperature cell transfer device 10 according to an embodiment of the present invention introduces oxygen from the atmosphere and generates heat through a redox reaction of the metal so as to continuously supply the optimal culture temperature to the culture vessel even without power supply. The first container 100 containing the live animal cells 11 and the culture medium 12 is stored in the second container 200 equipped with the emitting heating unit 210, and a culture environment optimal for cell proliferation of the cells 11 contained in the first container 100 is maintained to maintain the cells. maintains its activity and viability.

In addition, the second container 200 further includes an opening and closing unit 220 that determines the inflow of atmospheric oxygen. The opening and closing portion 220 includes an inlet hole 221 that penetrates one side of the second container 200 and allows atmospheric oxygen to flow into the second container 200, a stopper 223 that selectively opens and closes the inlet hole 221, and is installed in the stopper 223. It consists of an opening and closing member 225 that blocks the inflow of oxygen when the internal temperature of the second container 200 rises.

In addition, the opening and closing member 225 is made of bimetal or temperature-responsive linear material. In addition, the opening and closing member 225 is characterized by being applied as a cylinder or piston.

Ultimately, the power-free constant temperature cell transfer device 10 of the present invention maintains the optimal culture temperature even without power supply, so that the second container 200 further includes an opening and closing unit 220 that determines the inflow of atmospheric oxygen, so that when the temperature of the container increases, It automatically blocks oxygen inflow to lower the temperature, and when it drops to the set temperature, oxygen is introduced to maintain the optimal temperature for cell culture.

Meanwhile, a third container 300 containing live animal cells 11 and culture medium 12 is installed in the second container 200 so that the first container 100 containing live animal cells 11 and culture medium 12 is maintained in a horizontal state.

As shown in FIG. 2, the third container 300 has a spherical shape and includes an inner container 310 in which a heavy object 340 is installed on the inner bottom and a first container 100 is placed on top of the heavy object 340, and an inner container 310 that has a spherical shape and has an inner container It consists of an outer container 330 that accommodates the container 310, and a plurality of wheels 320 installed to roll and rotate between the inner container 310 and the outer container 330.

Furthermore, the outer container 330 and the inner container 310 form a sphere by assembling the hemispherical first auxiliary container 300a and the second auxiliary container 300b, and a packing 300c is provided between the first auxiliary container 300a and the second auxiliary container 300b. do.

Additionally, the weight 340 consists of a fluid or a solid. In particular, the heavy object 340 is composed of a solid having its own weight and a fluid filled on top of the solid.

Ultimately, as shown in FIG. 5, the power-free constant temperature cell transfer device 10 of the present invention includes an inner container 310 in which a heavy object 340 is installed in the second container 200 so that the cell storage container can be transported while maintaining its horizontal state even when subjected to external shock. , a third container 300 consisting of an external container 330 for accommodating the inner container 310 and a plurality of wheels 320 installed to roll and rotate between the inner container 310 and the outer container 330 is installed to prevent the bag from shaking or tilting during transport. Even so, ensure that the first container 100 containing the living animal cells 11 and the culture medium 12 is maintained in a horizontal state.

Meanwhile, it further includes a fourth container 400 that hermetically stores the first container 100. The fourth container 400 is preferably an airtight container.

In particular, the first container 100 and the third container 300 have a carbon dioxide concentration of 1% to 30%.

As used herein, the terms approximately, substantially, etc. are used to mean at or close to the numerical value when manufacturing and material tolerances inherent in the stated meaning are presented, and are used to mean accurate or close to that numerical value to aid in the understanding of the present invention. Absolute values are used to prevent unscrupulous infringers from taking unfair advantage of the stated disclosure.

The present invention described above is not limited to the above-described embodiments and the accompanying drawings, and various substitutions, modifications, and changes are possible without departing from the technical spirit of the present invention, as is commonly known in the technical field to which the present invention pertains. It will be clear to those who have the knowledge of.

* Explanation of symbols for main parts of the drawing *
10: Non-power constant temperature maintenance cell transfer device
11: cells 12: culture medium
100: first container 101: space
110: sealed passage 120: circulation filter
121: Pipeline 122: Valve
123: Filter 124: Clip
130: scraper 131: blade
132: Culture groove 134: Metal body
200: second container 210: heating unit
220: opening and closing part 221: inlet hole
223: stopper 225: opening and closing member
300: Third container 310: Inner container
320: Wheel 330: External container
340: Heavy goods 310a, 330a: First auxiliary container
310b, 330b: Second auxiliary container 310c, 320c: Packing
400: Fourth container 500: Bag

Claims (20)

delete A third container containing living animal cells and culture medium is installed in a second container equipped with a heating unit that introduces oxygen from the atmosphere and releases heat through a redox reaction of the metal, so that the first container containing living animal cells and culture medium is maintained in a horizontal state,
Allows transfer of cells contained in the first container while maintaining their physiological activity temperature through the second container,
The tilt of the first container is minimized through the third container,
The first container provides a space that can selectively determine the sealed state, and allows culture medium to be injected, cell inoculated, detached, and obtained into the space while the space is closed, and the space is open. To enable culturing of cells,
The first container includes a sealed passage for bringing in and out fluids, gases, and cells from the outside into and out of the space,
A circulation filter capable of circulating gas necessary for cell culture in the space,
It consists of a scraper installed in the space and scraping the cells by moving them to remove the cells from the bottom of the space,
The third container is,
an inner container having a spherical shape, a heavy object installed on the inner floor, and a first container placed on top of the heavy object;
an outer container having a spherical shape and accommodating the inner container;
It consists of a plurality of wheels installed to roll and rotate between the inner container and the outer container,
A non-powered constant temperature maintenance cell transfer device further comprising a fourth container for sealing the first container.
A third container containing living animal cells and culture medium is installed in a second container equipped with a heating unit that introduces oxygen from the atmosphere and releases heat through a redox reaction of the metal, so that the first container containing living animal cells and culture medium is maintained in a horizontal state,
Through the second container, the cells contained in the first container can be transferred while maintaining their physiological activity temperature, and through the third container, tilt of the first container is minimized, while carbon dioxide in the air is stored in the third container. Fill with carbon dioxide at a higher concentration than the
The first container provides a space that can selectively determine the sealed state, and allows culture medium to be injected, cell inoculated, detached, and obtained into the space while the space is closed, and the space is open. To enable culturing of cells,
The first container includes a sealed passage for bringing in and out fluids, gases, and cells from the outside into and out of the space,
A circulation filter capable of circulating gas necessary for cell culture in the space,
It consists of a scraper installed in the space and scraping the cells by moving them to remove the cells from the bottom of the space,
The third container is,
an inner container having a spherical shape, a heavy object installed on the inner floor, and a first container placed on top of the heavy object;
an outer container having a spherical shape and accommodating the inner container;
It consists of a plurality of wheels installed to roll and rotate between the inner container and the outer container,
An unpowered constant temperature maintenance cell transfer device further comprising a fourth container for sealing the first container.
delete delete According to paragraph 2 or 3,
The first container is,
A non-powered constant temperature maintenance cell transfer device, characterized in that the carbon dioxide concentration is 1% to 30%.
According to paragraph 2 or 3,
The third container is,
A non-powered constant temperature maintenance cell transfer device, characterized in that the carbon dioxide concentration is 1% to 30%.
According to paragraph 2 or 3,
A non-powered constant temperature maintenance cell transfer device, characterized in that the temperature inside the first container is maintained at 22°C to 43°C through the heating unit.
According to paragraph 2 or 3,
The heating unit,
A non-power constant temperature maintenance cell transfer device characterized in that heat is generated at 40°C to 60°C through a redox reaction between atmospheric oxygen and metal introduced into the second container.
According to paragraph 2 or 3,
The heating unit,
A non-powered constant temperature maintenance cell transfer device characterized by a mixture of metal powder that generates heat by reacting with oxygen and an insulating material.
According to paragraph 2 or 3,
A non-powered constant temperature maintenance cell transfer device, characterized in that the second container further includes an opening and closing part that determines the inflow of atmospheric oxygen.
delete delete According to clause 10,
The insulation material is,
A non-powered constant temperature maintenance cell transfer device characterized by selectively mixing one or more from the group consisting of sawdust, salt, and moisture.
According to clause 11,
The opening and closing part,
an inflow hole that penetrates one side of the second container and allows atmospheric oxygen to flow into the second container;
A stopper that selectively opens and closes the inlet hole,
A non-powered constant temperature maintenance cell transfer device, characterized in that it consists of an opening and closing member installed on the stopper to block the inflow of oxygen when the internal temperature of the second container rises.
According to paragraph 2 or 3,
The outer container and inner container are,
A non-power constant temperature maintenance cell transfer device characterized in that a hemispherical first auxiliary container and a second auxiliary container are assembled to form a sphere, and packing is provided between the first auxiliary container and the second auxiliary container.
According to paragraph 2 or 3,
The heavy goods are,
A non-powered constant temperature maintenance cell transfer device, characterized in that it is made of fluid.
According to paragraph 2 or 3,
The heavy goods are,
A solid having its own weight,
A non-powered constant temperature maintenance cell transfer device, characterized in that it consists of a fluid filled in the upper part of the solid.
According to clause 15,
The opening and closing member is,
A non-powered constant temperature maintenance cell transport device characterized by being applied as a bimetal or temperature-responsive linear material.
According to clause 15,
The opening and closing member is,
A non-powered constant temperature maintenance cell transport device characterized by being applied as a cylinder or piston.

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