KR102691768B1 - Equipment and method of vernalization that seagrass seeds with low-temperature seawater running water system - Google Patents

Abstract

The present invention relates to a device capable of vernalizing Jalpi seeds in low-temperature seawater in a running-water manner and a method for vernalizing Jalpi seeds using the same. The low-temperature seawater flowing-type Jalpi seed vernalization treatment device includes a water tank containing seawater; A management tank in which seawater is supplied from the tank and Jalpi seeds for vernalization are accommodated; A piping unit connected to the water tank and the management water tank through which seawater moves; a cooling unit that maintains the water temperature of the water tank; A sensor unit including a temperature sensor for detecting the water temperature of the water tank or the management water tank and a water level sensor for detecting the water level of the water tank or the management water tank; and controlling the piping unit according to the water level detected by the water level sensor to maintain a constant water level in the water tank or management water tank, and controlling the cooling unit according to the water temperature detected by the temperature sensor to maintain the temperature of the water tank or management water tank. It includes a control unit that keeps it constant.
In addition, as the generated hydrogen sulfide is discharged along with the seawater flow, the occurrence of contamination by hydrogen sulfide is lower than in the refrigerated storage method, which improves the survival rate of Jalpi seeds. Compared to the refrigerated storage method, the longitudinal section of Jalpi seeds has a thinner seed coat thickness. , germination rate and moisture content are also improved.

Description

Low-temperature seawater flow-type jalpi seed vernalization treatment device and method {Equipment and method of vernalization that seagrass seeds with low-temperature seawater running water system}

The present invention relates to a device capable of vernalizing Jalpi seeds using low-temperature seawater flow and a method for vernalization of Jalpi seeds using the same.

In general, Jalpi, a type of seaweed, is a marine flowering plant that blooms and fertilizes to form fruits. The underground stem and root system of Jalpi are natural purifiers that stabilize sediments and can absorb and remove pollutants such as heavy metals in sediments. It has a function.

In addition, due to its excellent photosynthesis, the Jalpi habitat is used as a spawning and nursery for marine life, and holds information on biodiversity and coastal environments, occupying an indispensable position in estuarine and coastal ecosystems.

However, recent marine pollution and physical destruction of the coastal environment are accelerating the devastation of jalpi communities, raising serious problems in terms of marine environmental conservation and fishery resource protection. In addition, there is a worldwide trend that jalpi communities are being destroyed due to indiscriminate coastal development and environmental pollution in coastal fishing grounds, and various marine biological resources are decreasing.

Therefore, to solve these problems, research on Jalpi forest creation and management technology using Jalpi seeds is being actively conducted around the world.

In this regard, a representative example of the development of seed management technology using Jalpi seeds is the vernalization method using refrigerated storage.

However, the vernalization method using refrigerated storage has a low germination rate and moisture content of Jalpi seeds, requires continuous exchange of air and seawater to suppress the generation of hydrogen sulfide, is complicated in securing refrigerated processing supplies and space, and is bulky, making it impractical. There is a problem that this is not possible.

Therefore, there is a need for technology for a vernalization treatment method for Jalpi seeds that is easier than refrigerated storage and can improve the germination rate and moisture content of seeds.

Korean Patent No. 10-0480526 (2005.03.24, Artificial cultivation method of Jalpi)

The present invention is intended to solve the above problems, and is intended to devise a vernalization treatment method for Jalpi seeds that is easier to manage than the refrigerated storage method and can improve the germination rate and moisture content.

In order to solve the above problem, the low-temperature seawater flowing-type Jalpi seed vernalization treatment device according to an embodiment of the present invention includes a water tank containing seawater; A management tank in which seawater is supplied from the tank and Jalpi seeds for vernalization are accommodated; A piping unit connected to the water tank and the management water tank through which seawater moves; a cooling unit that maintains the water temperature of the water tank; A sensor unit including a temperature sensor for detecting the water temperature of the water tank or the management water tank and a water level sensor for detecting the water level of the water tank or the management water tank; and controlling the piping unit according to the water level detected by the water level sensor to maintain a constant water level in the water tank or management water tank, and controlling the cooling unit according to the water temperature detected by the temperature sensor to maintain the temperature of the water tank or management water tank. It may include a control unit that keeps it constant.

In addition, the management water tank is blackened so that the amount of light can be maintained at 0 lux.

In addition, the piping unit includes a water intake pipe that intakes seawater into the water tank; a supply pipe connecting the water tank and the management water tank and supplying seawater in the water tank to the management water tank; And it may include a drain pipe through which seawater from the management tank is drained to the outside.

In addition, the cooling unit includes a circulation pipe through which seawater in the water tank circulates; A circulation pump that generates pressure to circulate seawater through the circulation pipe; and a cooler that cools the temperature of seawater circulating in the circulation pipe, and the control unit can control the cooler so that the temperature detected by the temperature sensor is maintained at a set temperature.

Additionally, the temperature set in the control unit may be 4°C.

In addition, the low-temperature seawater flow-through purification treatment device includes a filter that filters impurities contained in seawater taken in through the water intake pipe; It may further include a filtration and sterilization unit including a sterilizer that sterilizes seawater taken in through the water intake pipe with ultraviolet rays.

In addition, the filtration sterilization unit may further include an impurity discharger that discharges the filtered impurities.

In addition, the vernalization treatment method using the low-temperature seawater flow type Jalpi seed vernalization treatment device includes the step of receiving Jalpi seeds in a management tank; A control step of setting the water level of the water tank and the management water tank and the cooling temperature of the cooling unit; Filtration and sterilization steps to filter and sterilize impurities in natural seawater; A water tank intake step of taking filtered and sterilized seawater up to a set height in the water tank; A seawater cooling step of cooling and maintaining the seawater in the water tank at a set temperature; A management water tank water intake step of dispensing the cooled seawater to a set height in the management water tank; A seawater outflow step of discharging seawater when the seawater in the management tank reaches a set level; And a vernalization treatment step of vernalizing the Jalpi seeds contained in the management tank for a certain period of time, wherein the vernalization treatment step ensures that seawater is continuously taken in and drained into the water tank and the management tank during the vernalization treatment period. The management tank can be maintained at a certain water level and temperature.

In the low-temperature seawater flow-type Jalpi seed vernalization treatment device and the Jalpi seed vernalization treatment method using the same according to an embodiment of the present invention, the generated hydrogen sulfide is discharged together with the seawater flow, so the occurrence of contamination by hydrogen sulfide is lower than the refrigerated storage method, and the Jalpi seed vernalization treatment method is low. Survival rate can be improved.

In addition, compared to the refrigerated storage method, the thickness of the seed coat of Jalpi seeds is thinner, and the germination rate and moisture content can also be improved.

Figure 1 is an exemplary view of a low-temperature seawater flowing-type Jalpi seed vernalization treatment device according to an embodiment of the present invention.
Figure 2 is a graph comparing the germination rate by vernalization treatment period and color according to the observation date of the Example and Comparative Example of the present invention.
Figure 3 is a graph comparing the moisture content of the Example of the present invention and the Comparative Example.
Figure 4 is an observation of a longitudinal cross section of the embodiment.
Figure 5 is an observation of a longitudinal cross section of a comparative example.
Figure 6 shows a cross-sectional view of the example.
Figure 7 is an observation of a cross section of a comparative example.
Figure 8 is a flow chart of a jalpi seed vernalization treatment method using a low-temperature seawater flow type jalpi seed vernalization treatment device according to an embodiment of the present invention.

Hereinafter, the description of the present invention with reference to the drawings is not limited to specific embodiments, and various changes may be made and various embodiments may be possible. In addition, the content described below should be understood to include all conversions, equivalents, and substitutes included in the spirit and technical scope of the present invention.

Like reference numerals used throughout this specification refer to like elements.

As used herein, singular expressions include plural expressions, unless the context clearly dictates otherwise. In addition, terms such as “comprise,” “provide,” or “have” used below are intended to designate the presence of features, numbers, steps, operations, components, parts, or a combination thereof described in the specification. It should be construed and understood as not precluding the possibility of the presence or addition of one or more other features, numbers, steps, operations, components, parts or combinations thereof.

Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as generally understood by a person of ordinary skill in the technical field to which the present invention pertains. Terms such as those defined in commonly used dictionaries should be interpreted as having meanings consistent with the meanings they have in the context of the related technology, and should not be interpreted in an idealized or overly formal sense unless explicitly defined in the present application. No.

In addition, when describing with reference to the accompanying drawings, identical components will be assigned the same reference numerals regardless of the reference numerals, and overlapping descriptions thereof will be omitted. In describing the present invention, if it is determined that a detailed description of related known technologies may unnecessarily obscure the gist of the present invention, the detailed description will be omitted.

Hereinafter, with reference to FIGS. 1 to 7, a low-temperature seawater flowing-type jalpi seed vernalization treatment device according to an embodiment of the present invention will be described in detail.

Figure 1 is an exemplary view of a low-temperature seawater flowing-type Jalpi seed vernalization treatment device according to an embodiment of the present invention.

Referring to Figure 1, the low-temperature seawater flow-type Jalpi seed vernalization treatment device according to an embodiment of the present invention includes a water tank (10), a management water tank (20), a piping section (30), a cooling section (40), and a sensor section (50). , a control unit 60, and a filtration and sterilization unit 70.

The water tank 10 is a place to accommodate seawater, and is preferably of a size that can accommodate 1 ton or more of natural seawater, but is not limited thereto.

The management tank 20 is supplied with seawater from the tank 10 and can accommodate jalpi seeds for vernalization treatment.

The size of the management tank (20) is preferably 80 to 100 cm in height and 40 to 50 cm in diameter, but it is not limited to this. A seed storage container that is separable from the management tank (20) is included inside the management tank (20), allowing you to store seeds. It can be easily put into or taken out of the management tank (20).

Here, the seed storage container partially includes a form such as a sieve or net, so that seawater can be separated when the seed storage container is separated from the management water tank 20.

In addition, the side of the management tank (20) can be blackened with a blackout sheet, etc., and after storing the seeds inside the management tank (20), the top is covered with blackout vinyl or a blackout-treated cover to form the management tank (20). The amount of internal light can be maintained at 0 lux.

Additionally, there may be a plurality of management water tanks 20 connected to one water tank 10.

The piping unit 30 is connected to the water tank 10 and the management water tank 20 through which seawater can move, and may include a water intake pipe 31, a supply pipe 32, and a drain pipe 33.

In detail, seawater is supplied to the water tank 10 through the water intake pipe 310, and the seawater in the water tank 10 is supplied to the water tank 10 through the supply pipe 32 connected to the water tank 10 and the management water tank 20. ), and the seawater in the management tank 20 can be discharged to the outside through the drain pipe 33.

The cooling unit 30 can maintain the water temperature of the water tank 10 at a constant level and may include a circulation pipe 41, a circulation pump 42, and a cooler 43.

In detail, when the circulation pump 42 generates pressure, the seawater in the water tank 10 circulates through the circulation pipe 41, and the cooler 43 cools the temperature of the seawater circulating through the circulation pipe 41. You can.

At this time, the temperature of seawater is preferably 4°C, but is not limited thereto.

The sensor unit 50 may include a temperature sensor 51 that detects the water temperature in the water tank 10 or the management water tank 20 and a water level sensor 52 that detects the water level in the water tank 10 or the management water tank 20. You can.

In detail, a temperature sensor 51 is installed in the water tank 10 or the management water tank 20 to check whether the water temperature in the water tank 10 or the management water tank 20 is maintained constant, and the water tank 10 Alternatively, a water level sensor may be installed in the management water tank 20 to check whether the water level in the water tank 10 or the management water tank 20 is maintained at a set level.

The set values of the temperature sensor 51 and the water level sensor 52 described above can be set in the control unit 60, and can be controlled to maintain the set values.

In detail, when the water temperature of the water tank 10 or the management water tank 20 detected by the temperature sensor 51 differs from the set value, the cooling unit 40 is controlled to cool the water tank 10 or the management water tank 20. The temperature can be maintained at the set value.

Additionally, if the water level detected by the water level sensor 52 is different from the set value, the piping unit 30 can be controlled to maintain the water level in the water tank 10 or the management water tank 20 at the set value.

The cooling unit 40 can be controlled by controlling the circulation pump 42 to quickly circulate seawater or by controlling the cooler 43 to control the cooling temperature of seawater.

The filtration and sterilization unit 70 can filter and sterilize seawater taken into the water tank 10, and may include a filter 71, a sterilizer 72, and an impurity discharger 73.

In detail, the filter 71 can filter out impurities contained in seawater taken into the water tank 10, and the sterilizer 72 is a device that can sterilize, such as an ultraviolet sterilizer, to filter out the seawater flowing into the water tank 10. It can be sterilized, and impurities filtered from the filter 71 can be discharged to the outside using the impurity discharger 73.

At this time, the impurity discharger 73 may be used to manually or automatically discharge impurities accumulated in a certain space, but this is only an example and the form of the impurity discharger 73 is not limited.

Therefore, the filtration and sterilization unit 70 is preferably installed at both ends or inside the water intake pipe 31 so that filtered and sterilized seawater can flow into the water tank 10.

Figure 8 is a flowchart of a jalpi seed vernalization treatment method using a low-temperature seawater flow type jalpi seed vernalization treatment device according to an embodiment of the present invention.

Referring to Figure 8, the Jalpi seed vernalization treatment method includes the Jalpi seed receiving step (S1), the control step (S2), the filtration and sterilization step (S3), the water tank intake step (S4), the seawater cooling step (S5), and the management water tank. It may include a water intake step (S6), a seawater outflow step (S7), and a vernalization step (S8).

In detail, the Jalpi seed receiving step (S1) of placing Jalpi seeds in a seed storage container and then storing them in the management tank 20, the water level and cooling unit of the water tank 10 and the management tank 20 in the control unit 60 ( The control step (S2) of setting the cooling temperature of 40) may be performed first.

When the setting of the control unit 60 is completed, natural seawater flows into the water intake pipe 31, impurities such as stones, seaweed, and sand are filtered through the filter 71, and microorganisms, bacteria, or bacteria in the seawater are removed through the sterilizer 72. A filtration and sterilization step (S3) in which viruses, etc. are sterilized may be performed.

Seawater that passes through the water intake pipe 31 and has been filtered and sterilized flows into the water tank 10, and a water intake step (S4) in which the water tank is filled to the water level set by the control unit 60 may proceed.

When the water tank 10 is filled, the circulation pump 42 operates so that the seawater in the water tank 10 can flow into the circulation pipe 41, and is cooled to the temperature set in the control unit 60 through the cooler 43. It may flow into the water tank 10 again.

As described above, the seawater cooling step (S5) in which the seawater in the water tank 10 is continuously circulated through the cooling unit 40 and maintained at the temperature set by the control unit 60 may proceed.

When the temperature sensor 51 detects that the seawater in the water tank 10 has reached the water temperature set by the control unit 60, the seawater in the water tank 10 may flow into the management water tank 20 through the supply pipe 32. .

The management water tank 20 can accommodate seawater flowing in from the supply pipe 32, and a management water intake step (S6) in which the management water tank is filled to the water level set by the control unit 60 can proceed.

When the management tank 20 is filled to the water level set by the control unit 60, the seawater outflow step (S7) in which the seawater in the management tank 20 flows out through the drain pipe 33 may proceed.

At this time, the amount of seawater supplied into the management tank 20 and the amount of seawater flowing out through the drain pipe 33 are the same, so the water level inside the management tank 20 can be maintained constant.

In addition, the seawater in the water tank 10 is refilled by the amount of seawater discharged into the management tank 20, so that the water level in the water tank 10 can also be maintained constant.

As described above, a vernalization treatment step (S8) may be performed in which cooled seawater is continuously flowed into the management tank 20 and vernalization of Jalpi seeds is performed for a certain period of time. During the vernalization treatment period during which the vernalization treatment step (S8) is performed, The seawater in the management tank 20 can maintain a constant water level and temperature.

Hereinafter, the germination rate, moisture content, and longitudinal cross-sectional observation results of Jalpi seeds vernalized using a low-temperature seawater flow-type Jalpi seed vernalization treatment device and Jalpi seeds vernalized using a refrigerated storage method according to an embodiment of the present invention are compared in Experiments 1 to 3. Let's compare.

[Example]

The examples are jalpi seeds that were vernalized by maintaining the temperature of the seawater at 4°C in a dark room condition of 0 lux using a low-temperature seawater running-type jalpi seed vernalization treatment device according to an embodiment of the present invention.

[Comparative example]

The comparative example is jalpi seeds vernalized using a refrigerated storage method, and stored in a refrigerator maintained at 4°C in a dark room of 0 lux.

[Experiment 1]

Experiment 1 is an experiment to check the germination rate by vernalization treatment period and color in the experimental examples and comparative examples.

In Examples and Comparative Examples, vernalization treatment was started on the same day, cultured indoors on the same day, and observed at intervals of 3 to 4 days for about 4 months.

Here, the vernalization treatment period was set at 0 days, 5 days, 10 days, 15 days, and 30 days. For convenience, the day when the first germination was observed was set as the first time, and the results of 12 observations were recorded until the end of the experiment.

Germination of seeds was first observed 80 days after the start of the experiment, and was judged to have germinated when the seed coat opened and the embryo came out.

1st round 2nd round 3rd session 4th session 5th session 6th session 7th session 8th session 9th session 10th session 11th session 12th session 0 days - - - - - - One 2 2 2 2 2 5 days One 3 3 3 3 3 3 4 4 4 5 6 10 days - 2 2 2 2 2 2 3 3 5 5 6 15th - - - - - - One 5 6 6 6 6 30 days One One One One One 2 4 4 5 5 8 8

Table 1 shows the germination rate (%) of Jalpi seeds by vernalization treatment period according to the observation date of the Example, and the color of Jalpi seeds was selected as Golden Brown.

1st round 2nd round 3rd session 4th session 5th session 6th session 7th session 8th session 9th session 10th session 11th session 12th session 0 days - - - - - - - - - - One One 5 days One One One One One One One 2 2 3 3 3 10 days - One One One One One 2 2 2 2 2 2 15th - 2 2 2 2 2 2 2 4 4 4 4 30 days - - - - - One 3 3 3 5 5 5

Table 2 shows the germination rate (%) of Jalpi seeds by vernalization treatment period according to the observation date of the comparative example, and the color of Jalpi seeds was selected as Golden Brown.

Referring to Table 1 and Table 2, as a result of the 12th observation, the Example showed a germination rate of 2 to 8%, and the Comparative Example showed a germination rate of 1 to 5%.

Figure 2 is a graph comparing the germination rate by vernalization treatment period and color according to the observation date of the Examples and Comparative Examples of the present invention.

Referring to Figure 2, it can be seen that, except for day 0 of the vernalization condition, the maximum germination rate of Jalpi seeds observed in the 12th round is higher in the Example than the Comparative Example, and it can be seen that the germination rate of Golden Brown color is overall high. .

[Experiment 2]

Experiment 2 is an experiment to compare the moisture content according to the color of the seeds of Examples and Comparative Examples. After vernalization of the Examples and Comparative Examples for 5 days, the water temperature was 15°C and the light amount was 80 ㎛ol·m-2·s-1. , photoperiod (12L:12D), and salinity of 30 psu were stored under indoor culture conditions and observed once a week.

As a result of the experiment, referring to FIG. 3, the initial moisture content of the example was about 38 to 46%, which did not show much difference from the comparative example. However, at the 10th time, two months after the start of the experiment, the example showed a moisture content of 53.2 to 82.8%. In the comparative example, the moisture content was 51.4 to 75.7%, showing that the Jalpi seeds of the example had a relatively high moisture content.

[Experiment 3]

Experiment 3 was to observe the embryo formation process just before germination for each color of Jalpi seeds. After vernalization of the Jalpi seeds of Examples and Comparative Examples for 5 days, the water temperature was 15°C and the light amount was 80㎛ol·m-2·s-1. , photoperiod (12L:12D), and salinity of 30psu were transferred to culture conditions and stored stationary. The first observation was made after 25 days of storage, once a month, a total of 4 times.

Figure 4 is an observation of a longitudinal cross section of the Example, Figure 5 is an observation of a longitudinal cross section of the Comparative Example, Figure 6 is an observation of a cross section of the Example, and Figure 7 is an observation of a cross section of the Comparative Example.

Referring to Figure 4, it can be seen that the empty space inside the seed is generally small in the example, and the diameter of the abdomen increases from the second time.

In addition, in the 3rd and 4th rounds, it can be seen that the length growth and differentiation of the embryo part are clear, and the pores of the endosperm part have become very dense.

In comparison, referring to FIG. 5, it can be seen that the comparative example has a relatively large amount of empty space inside the seed compared to the example, and the growth and differentiation of the pear is not as clear as that of the example.

Referring to Figure 6, in the example, the early embryo development can be confirmed in all colors in the first round, and from the second round, the belly part begins to develop rapidly in all colors, and in the third round, it can be seen that the belly part has significantly expanded. .

In addition, in the 4th time, it can be seen that in all colors, the abdomen has expanded to just below the seed coat and is in the form just before germination.

In comparison, referring to Figure 7, in the comparative example, the initial form of embryo development can be confirmed in colors except Dark Brown in the first round, and the shape of the embryo can be confirmed in all colors in the second round, but Green Cyan to Oyster and In Golden Brown color, you can see that there is relatively more empty space inside the seed.

Afterwards, from the 3rd time, the abdomen actively expanded, and in the 4th time, it can be seen that the abdomen expanded to the bottom of the seed coat.

Through the above experiments 1 to 3, it was confirmed that the Example was superior to the Comparative Example in terms of germination rate and moisture content, and that the elongation of the pear occurred much more actively, thereby confirming that the Example was superior to the Comparative Example in terms of germination rate and water content, and that the elongation of the pear occurred much more actively. Thus, the Example was confirmed to be superior to the Comparative Example in terms of germination rate and moisture content, and the elongation of the pear occurred much more actively. Thus, it was confirmed that the Example was superior to the Comparative Example in terms of germination rate and moisture content, and that the elongation of the pear was much more active. The effectiveness of the vernalization method was proven.

Although embodiments of the present invention have been described above with reference to the attached drawings, those skilled in the art will understand that the present invention can be implemented in other specific forms without changing the technical idea or essential features of the present invention. You will be able to understand it. Therefore, the embodiments described above are illustrative in all respects and are not restrictive.

1: Jalpi seed low-temperature seawater flowing-through vernalization treatment device
10: Water tank
20: Management tank
30: Plumbing department
31: water intake pipe
32: supply pipe
33: drain pipe
40: Cooling unit
41: circulation pipe
42: Circulation pump
43: cooler
70: Filtration and sterilization unit
71: Filter
72: Sterilizer
73: Impurity discharger
S1: Jalpi seed acceptance stage
S2: Control phase
S3: Filtration and sterilization step
S4: Water tank intake step
S5: Seawater cooling step
S6: Management tank water intake step
S7: Seawater outflow phase
S8: Vernalization step

Claims (8)

A tank containing seawater;
A management tank in which seawater is supplied from the tank, Jalpi seeds for vernalization are accommodated, and the amount of light is maintained at 0 lux by being blackened;
A piping unit connected to the water tank and the management water tank through which seawater moves;
a cooling unit that maintains the water temperature of the water tank;
A sensor unit including a temperature sensor for detecting the water temperature of the water tank or the management water tank and a water level sensor for detecting the water level of the water tank or the management water tank; and
The piping part is controlled according to the water level detected by the water level sensor to keep the water level of the water tank or management water tank constant, and the cooling part is controlled according to the water temperature detected by the temperature sensor to keep the temperature of the water tank or management water tank constant. It includes a control unit that maintains
The management tank is,
Inside, it includes a seed storage container that is separable from the management tank and is formed in the form of a sieve or net,
The piping department,
A water intake pipe for taking seawater into the water tank;
a supply pipe connecting the water tank and the management water tank and supplying seawater in the water tank to the management water tank; and
It includes a drain pipe through which seawater from the management tank is drained to the outside,
The cooling unit,
A circulation pipe through which seawater circulates in the water tank;
A circulation pump that generates pressure to circulate seawater through the circulation pipe; and
It includes a cooler that cools the temperature of the seawater circulating in the circulation pipe,
The control unit is a low-temperature seawater flow type Jalpi seed vernalization treatment device, characterized in that it controls the cooler so that the temperature detected by the temperature sensor is maintained at 4°C.
delete delete delete delete According to paragraph 1,
The low-temperature seawater flow-type Jalpi seed vernalization treatment device,
A filter that filters impurities contained in seawater taken in through the water intake pipe;
A low-temperature seawater flow-type Jalpi seed vernalization treatment device further comprising a filtration sterilization unit including a sterilizer for ultraviolet ray sterilization of seawater taken in through the water intake pipe.
According to clause 6,
The filtration and sterilization unit,
A low-temperature seawater flow-type Jalpi seed vernalization treatment device further comprising an impurity discharger for discharging filtered impurities.
A Jalpi seed receiving step of accommodating the Jalpi seeds in a seed storage container in the management tank;
A control step for setting the water level of the water tank and the management water tank and the cooling temperature of the cooling unit;
Filtration and sterilization steps to filter and sterilize impurities in natural seawater;
A water tank intake step of taking filtered and sterilized seawater up to a set height in the water tank;
A seawater cooling step of cooling and maintaining the seawater in the water tank at a set temperature;
A management water tank water intake step of dispensing the cooled seawater to a set height in the management water tank;
A seawater outflow step of discharging seawater when the seawater in the management tank reaches a set level; and
It includes a vernalization treatment step of vernalizing the Jalpi seeds contained in the management tank for a certain period of time,
The vernalization step is,
A low-temperature seawater flow-type Jalpi seed vernalization treatment method, characterized in that the water intake and drainage of seawater continues to be carried out in the water tank and the management tank during the vernalization treatment period so that the management water tank maintains a constant water level and a constant water temperature.