KR102785965B1 - A Cosmetic composition for skin whitening comprising Rice bran extract, Fig extract, Nymphaea Alba flower extract and Yuja extract - Google Patents

Abstract

One aspect relates to a composition for antioxidation or skin whitening, comprising a rice bran extract, a fig extract, a Nymphaea Alba extract, and a citron extract. The composition according to one aspect has excellent radical scavenging activity, tyrosinase activity inhibition activity, and melanin production inhibition activity, and therefore exhibits excellent effects for antioxidation and skin whitening. Therefore, the composition can be usefully utilized in cosmetics, external skin preparations, etc. for antioxidation and skin whitening.

Description

{A Cosmetic composition for skin whitening comprising Rice bran extract, Fig extract, Nymphaea Alba flower extract and Yuja extract}

The present invention relates to a cosmetic composition comprising rice bran extract, fig extract, Nymphaea Alba extract, and yuzu extract.

In modern society, as the standard of living improves, interest in beauty as well as health is gradually increasing. Among these, product development is actively underway to improve pigmentation-type skin diseases such as melasma and freckles caused by skin damage and aging due to ultraviolet rays, air pollution, etc., and to improve overall skin tone.

Melanin, which exists in the basal layer of the epidermis, is the main pigment that determines skin color, and protects the skin by preventing damage from ultraviolet rays and removing active oxygen. However, if melanin is overproduced locally, it causes skin diseases such as blemishes, freckles, dark spots, and skin cancer. In addition, if melanin that is overproduced continuously remains in the dermis, it turns the skin color black.

Human skin color is affected by the presence or absence of pigments such as skin melanin, hemoglobin, and carotenoids produced by melanocytes, which are pigment cells present in the epidermis, as well as the thickness and reflectivity of the skin. Of these, the melanin pigment, the most important factor in determining skin color, is formed when tyrosine, a type of amino acid that normally exists in the human body, is converted to DOPA by tyrosinase, an enzyme present in melanocytes, and through a series of complex oxidation processes, ultimately creates melanin, a black-brown polymer.

Specifically, the production of melanin is carried out by using tyrosine, an amino acid, as a substrate, and converting it into DOPA (3,4-dihydroxyphenylalanine) by tyrosinase, TRP-1 (tyrosinase-related protein-1), and TRP-2 (tyrosinase-related protein-2), and then into DOPA quinone, which then undergoes a non-enzymatic reaction and spontaneous oxidation process, followed by a polymerization reaction with an amino acid or protein.

Research has continued to find substances that exhibit skin whitening effects by inhibiting melanin production, but arbutin and kojic acid, which are currently the most commonly used whitening agents, are known to have side effects such as skin irritation.

Accordingly, the inventors of the present invention seek to provide a skin whitening cosmetic composition comprising a mixture of natural extracts, including rice bran extract, fig extract, lotus flower extract, and citron extract, which exhibit a whitening effect while having relatively few side effects.

Korean Patent Publication No. 10-2024-0144502

One aspect is to provide a cosmetic composition comprising rice bran extract, fig extract, Nymphaea Alba extract, and yuzu extract.

Another aspect is to provide an antioxidant or skin whitening topical skin preparation comprising rice bran extract, fig extract, lotus seed extract and yuzu extract.

Another aspect provides a method for preparing a cosmetic composition comprising a step of mixing rice bran extract, fig extract, lotus seed extract and yuzu extract.

One aspect provides a cosmetic composition comprising rice bran extract, fig extract, Nymphaea Alba extract, and yuzu extract.

The above "rice bran" is a mixture of the pulverized pericarp, seed coat, and aleurone layer that are produced when brown rice is polished to make white rice, and its quantity increases as the degree of polishing of brown rice increases. In addition, the components of rice bran also change depending on the degree of polishing, because the components of brown rice change as they move from the outer layer to the inner layer. For example, when the degree of polishing is high, fat, vitamin B1, and phosphorus increase and fiber decreases. Rice bran has high industrial utility value because it is a byproduct discarded during the polishing process.

The above "fig" is a subtropical semi-arboreal deciduous broad-leaved tree belonging to the mulberry family, with over 600 species distributed worldwide, and is known as the first fruit used by humans throughout history. The above fig has been recorded in the Bible and Donguibogam for its folk medical use since ancient times, and is known to be good for strengthening blood pressure, strengthening the stomach, nourishing, treating constipation, hepatitis, cancer, gynecological diseases, and restoring vitality. The general efficacy of the above fig is its action as a gastrointestinal medicine, treating diarrhea and hemorrhoids, sore throat, and promoting digestion, and it is also used as an antihelminthic and for jaundice.

The above " Nymphaea Alba " is a plant of the Nymphaea family, also called white water lily and European white water lily. It contains active alkaloids, nupharin and nymphaeine, and is used as a sedative. In herbal medicine, the roots and stems are used along with the flowers, and the efficacy of the flower part is excellent. In particular, alcohol extracts can extract alkaloid components in high concentration, so they have an excellent sedative effect.

The above "Yuja" belongs to the Rutaceae and Citrus genus, and is known to be good for bronchial diseases, asthma, colds, and skin care, prevent aging and fatigue, prevent cerebrovascular disorders and stroke, and have a purulent and excretory effect, helping to expel waste accumulated in the body.

In one specific example, the rice bran, fig, lotus seed and/or citron may be a material derived from all, part or a combination thereof.

In one specific example, the rice bran may be at least one selected from the group consisting of the pericarp, seed coat, and bran layer obtained by polishing brown rice, and specifically may include all of the pericarp, seed coat, and bran layer obtained by polishing brown rice.

In one specific example, the fig may be at least one selected from the group consisting of buds, roots, stems, branches, leaves, whole plants, peels, flowers, fruits and seeds of a fig tree, and specifically, may be a fruit of a fig tree.

In one specific example, the part of the azalea flower may be at least one selected from the group consisting of a bud, root, stem, branch, leaf, whole plant, bark, flower, fruit, and seed of the azalea flower, and specifically, may be a flower of the azalea flower.

In one specific example, the citron may be at least one selected from the group consisting of buds, roots, stems, branches, leaves, whole plants, bark, flowers, fruits and seeds of a citron tree, and specifically, may be a fruit of a citron tree.

The above rice bran, fig, lotus root and/or yuzu can be purchased and used commercially or used as those collected or grown in nature.

In one specific example, the extract may be extracted with water, an alcohol having 1 to 5 carbon atoms, hexane, chloroform, butylene glycol or a mixed solvent thereof, specifically, may be extracted with water, an alcohol having 1 to 5 carbon atoms or a mixed solvent thereof, and more specifically, may be extracted with water, ethanol or a mixed solvent thereof.

Additionally, the alcohol concentration of the mixture of alcohol having 1 to 5 carbon atoms and water may be 1 to 100% (v/v), for example, 10 to 100% (v/v), 20 to 100% (v/v), 30 to 100% (v/v), 40 to 100% (v/v), 50 to 100% (v/v), 60 to 100% (v/v), 70 to 100% (v/v), 60 to 90% (v/v), 60 to 80% (v/v).

The term "extract" refers to the results, such as a liquid component obtained by immersing a target substance in various solvents and then extracting it at room temperature or in a heated state for a certain period of time, and a solid component obtained by removing the solvent from the liquid component. In addition, it can be interpreted to include, in addition to the results, a dilution of the results, a concentrate thereof, a conditioning agent thereof, the extract itself, and all formulations of the extract that can be formed using the extract. Accordingly, the extract can be interpreted to include an extract obtained by extraction treatment, a dilution or concentrate of the extract, a dried product obtained by drying the extract, a conditioning agent or purified product of the extract, or a mixture thereof, and an extract itself and all formulations of the extract that can be formed using the extract.

In one specific example, the extraction method of the extract may be performed by using a single method or a combination of two or more methods according to a method commonly used in the art. Examples of the extraction method include a heating extraction method, a hot water extraction method, a cold infusion extraction method, a reflux cooling extraction method, a solvent extraction method, a steam distillation method, an ultrasonic extraction method, an extraction method, a pressing method, a subcritical extraction method, a supercritical extraction method, etc.

In one specific embodiment, the cosmetic composition may have one or more activities selected from the group consisting of antioxidant and skin whitening.

The term "antioxidant" refers to the activity of preventing and improving cell aging and skin oxidation by removing active oxygen, the main cause of skin aging.

In one specific example, the cosmetic composition may have an effect of preventing or improving skin aging by improving skin oxidation, i.e., a skin antioxidant effect. At this time, the skin aging may be at least one selected from the group consisting of wrinkles, decreased skin elasticity, blemishes, freckles, skin damage caused by ultraviolet rays, dry skin, weakening of the skin barrier, weakening of skin transparency, keratinization, damage to skin texture, and skin cancer, and specifically, may be at least one selected from the group consisting of wrinkles, skin damage caused by ultraviolet rays, decreased skin elasticity, dry skin, weakening of the skin barrier, weakening of skin transparency, keratinization, and damage to skin texture, and more specifically, may be at least one selected from the group consisting of skin damage caused by ultraviolet rays, wrinkles, and decreased skin elasticity.

In one specific example, the antioxidant activity may be radical scavenging activity.

In one embodiment, when a mixed extract including rice bran extract, fig extract, water lily flower extract, and citron extract was used, excellent DPPH radical scavenging activity was exhibited, and in particular, when rice bran extract, fig extract, water lily flower extract, and citron extract were combined in a weight ratio of 2: 2: 1: 1, a synergistic effect occurred and the highest DPPH radical scavenging activity was exhibited. That is, it was confirmed that a significantly excellent antioxidant activity was exhibited in a mixed extract having the composition of the above weight ratio (see Experimental Example 1).

In one specific example, the skin whitening activity may be at least one selected from the group consisting of tyrosinase activity inhibition ability and melanin production inhibition ability, and specifically, may be both tyrosinase activity inhibition ability and melanin production inhibition ability.

The above "skin whitening" activity is induced by inhibiting the activity of the tyrosinase enzyme or promoting the reduction of already produced melanin pigment. Tyrosine, which is produced in melanosomes, which are melanin cells present in the epidermis, the surface of the skin, is a precursor of melanin and is synthesized into melanin via DOPA-quinone. An enzyme called tyrosinase acts in this intermediate process, and when the activity of tyrosinase, which is involved in the process of tyrosine being oxidized into DOPA and dopachrome, is inhibited, melanin production is inhibited, and accordingly, a whitening effect that brightens the skin tone appears.

The above "tyrosinase" is an enzyme that promotes melanin production, converting tyrosine, which is the rate-controlling step in melanin synthesis, into DOPA (3,4-dihydroxyphenylalanine), and then mediating the conversion into DOPA-quinone.

In one specific example, the skin whitening activity may be to alleviate one or more selected from the group consisting of freckles, liver spots, lentigines, moles, café au lait spots, nevus of Ota, blue nevus, hyperpigmented spots, post-drug hyperpigmentation, gravidic chloasma, and post-inflammatory hyperpigmentation due to injury or dermatitis.

In one embodiment, when a mixed extract including rice bran extract, fig extract, water lily flower extract, and citron extract was used, excellent tyrosinase inhibitory activity and cellular melanin production inhibitory activity were exhibited, and in particular, when rice bran extract, fig extract, water lily flower extract, and citron extract were combined in a weight ratio of 2: 2: 1: 1, a synergistic effect occurred, exhibiting the highest tyrosinase inhibitory activity and cellular melanin production inhibitory activity. That is, it was confirmed that a remarkably excellent whitening effect was exhibited in the mixed extract having the composition of the above weight ratio (see Experimental Examples 2 and 3).

In one specific example, the cosmetic composition may contain rice bran extract, fig extract, lotus seed extract, and citron extract in a weight ratio of 10 to 1:10 to 1:1:1, and specifically, may contain it in a weight ratio of 6 to 1:6 to 1:1:1. For example, the cosmetic composition may contain rice bran extract, fig extract, lotus seed extract, and citron extract in a weight ratio of 6: 6: 1: 1, 6: 4: 1: 1, 6: 2: 1: 1, 6: 1: 1: 1, 4: 6: 1: 1, 4: 4: 1: 1, 4: 2: 1: 1, 4: 1: 1: 1, 2: 6: 1: 1, 2: 4: 1: 1: 1, 2: 2: 1: 1: 1, 2: 1: 1: 1, 1: 6: 1: 1, 1: 4: 1: 1, 1: 2: 1: 1: 1 or 1: 1: 1: 1. More specifically, the cosmetic composition may contain rice bran extract, fig extract, lotus seed extract, and yuzu extract in a weight ratio of 2:2:1:1.

When a cosmetic composition according to one aspect contains rice bran extract, fig extract, lotus seed extract, and citron extract in a weight ratio of 10 to 1:10 to 1:1:1, a synergistic effect occurs to exhibit high DPPH radical scavenging activity, tyrosinase inhibitory effect, and melanin production inhibitory effect, thereby exhibiting significantly excellent antioxidant and whitening effects. When the respective extracts are mixed in a weight ratio out of the above range, the synergistic effect may be reduced or not exhibited, thereby exhibiting low antioxidant and whitening effects.

In one specific embodiment, the cosmetic composition may be prepared in any formulation commonly prepared in the technical field to which the present invention pertains. For example, the cosmetic composition may be formulated as a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleanser, an oil, a powder foundation, an emulsion foundation, a wax foundation, a spray, and the like. Specifically, the cosmetic composition can be manufactured into a formulation including a toner such as a flexible toner or a nourishing toner, an emulsion such as a facial lotion or a body lotion, a cream such as a nourishing cream, a moisture cream, an eye cream, an essence, a cosmetic ointment, a spray, a gel, a pack, a sunscreen, a makeup base, a foundation such as a liquid type, a solid type or a spray type, a makeup remover such as a powder, a cleansing cream, a cleansing lotion, a cleansing oil, a cleanser such as a cleansing foam, a soap, a body wash, a liquid or gel formulation of mesotherapy that can be injected into the skin layer, and a formulation mixed with a hyaluronic acid gel. More specifically, the cosmetic composition can be manufactured into one or more formulations selected from the group consisting of a serum, a toner, an essence, a paste, a mask pack, a patch, a gel, a cream, a lotion, a nourishing lotion, a nourishing cream, a moisture cream, a massage cream, a powder, a soap, a cleanser, an oil, a foundation, a makeup base, a wax, and a spray.

The above cosmetic composition may further comprise any conventional cosmetic ingredient selected from additional ingredients commonly used in cosmetics, such as excipients, thickeners, dispersants, fragrances, fillers, preservatives, antiseptics, neutralizers, sweeteners, vitamins, free radical scavengers, metal ion sequestrants, functional ingredients and mixtures thereof. A person skilled in the art can select any additional ingredient and/or its amount such that the advantageous properties of the composition according to one aspect are not or are substantially not adversely affected by the expected addition.

For example, the excipients may include surfactants, emulsifiers, soap acids, solvents, colorants, preservatives, antioxidants, antifoaming agents, antibacterial agents, anti-redeposition agents, enzymes, plant or mineral oils, fats, fluorescent substances, fungicides, hydrotropists, moisturizers, fragrances, preservatives, proteins, silicones, solubilizers, sugar derivatives, sunscreens, vitamins, plant extracts, waxes, fatty acids, higher alcohols, hydrocarbon oils, ester oils, triglycerides, vegetable oils, and the like.

According to one aspect, the cosmetic composition may additionally include conventional auxiliary agents such as purified water, surfactants, moisturizers, lower alcohols, chelating agents, bactericidal agents, preservatives, antioxidants, stabilizers, solubilizers, vitamins, pigments, and fragrances, in addition to other components commonly added to cosmetic compositions.

In addition, the cosmetic composition may be appropriately mixed with ingredients commonly used in external skin preparations such as cosmetics or medicines, such as aqueous ingredients, oily ingredients, powder ingredients, alcohols, moisturizers, thickeners, ultraviolet absorbers, whitening agents, preservatives, antioxidants, surfactants, fragrances, colorants, various skin nutrients, or combinations thereof, as needed. The above cosmetic composition may also appropriately contain metal sequestering agents such as sodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid; caffeine, tannin, bellapamil, licorice extract, glablidine, hot water extract of the fruit of Kalin, various crude drugs, drugs such as tocopherol acetate, glycyrrhizic acid, tranexamic acid, and derivatives or salts thereof; and sugars such as vitamin C, magnesium ascorbic acid phosphate, ascorbic acid glucoside, arbutin, kojic acid, glucose, fructose, and trehalose.

When the above cosmetic composition is a surfactant-containing cleansing formulation, the carrier component may further include an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, methyl taurate, a sarcosinate, a fatty acid amide ether sulfate, an alkyl amidobetaine, a fatty alcohol, a fatty acid glyceride, a fatty acid diethanolamide, a vegetable oil, a linolenic derivative, or an ethoxylated glycerol fatty acid ester.

In addition, when the cosmetic composition according to one aspect is in the form of a cream or gel formulation, it may further include animal oil, vegetable oil, wax, paraffin, starch, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide as a carrier component.

In addition, when the cosmetic composition is in the form of a solution or emulsion, it may further contain a solvent, solvating agent or emulsifying agent such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, propylene glycol, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

If the above cosmetic composition is in the form of a suspension formulation, it may further include a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth as a carrier component.

If the above cosmetic composition is in a powder or spray formulation, it may further contain lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder as a carrier component, and particularly if it is in a spray formulation, it may further contain a propellant such as chlorofluorohydrocarbon, propane/butane or dimethyl ether.

The above cosmetic composition may be used by applying alone or in duplicate, or by applying in duplicate with another cosmetic composition other than the cosmetic composition according to one aspect. In addition, all ingredients included in the above cosmetic composition do not exceed the regulations set by each country. In addition, the cosmetic composition according to one aspect may be used according to a conventional method of use, and the number of times of use may vary depending on the user's skin condition or preference.

A cosmetic composition comprising a rice bran extract, a fig extract, a water lily extract and a citron extract according to a daily aspect exhibits significantly superior DPPH radical scavenging activity, tyrosinase inhibitory effect and melanin production inhibitory activity due to a synergistic effect compared to a single extract or a mixed extract of two or three extracts, and thus can exhibit superior antioxidant and skin whitening activities.

Another aspect provides an antioxidant or skin whitening topical skin preparation comprising rice bran extract, fig extract, lotus seed extract and yuzu extract.

The above “rice bran”, “fig”, “alba water lily”, “yuzu”, “extract”, “skin whitening”, etc. may be within the above-mentioned scope.

In one specific example, the skin external preparation may contain rice bran extract, fig extract, lotus seed extract, and citron extract in a weight ratio of 10 to 1:10 to 1:1:1, and specifically, may contain it in a weight ratio of 6 to 1:6 to 1:1:1. For example, the skin external preparation may contain rice bran extract, fig extract, lotus seed extract, and citron extract in a weight ratio of 6: 6: 1: 1, 6: 4: 1: 1, 6: 2: 1: 1, 6: 1: 1: 1, 4: 6: 1: 1, 4: 4: 1: 1, 4: 2: 1: 1, 4: 1: 1: 1, 2: 6: 1: 1, 2: 4: 1: 1: 1, 2: 2: 1: 1, 2: 1: 1: 1, 1: 6: 1: 1, 1: 4: 1: 1, 1: 2: 1: 1: 1 or 1: 1: 1: 1. More specifically, the skin external preparation may contain rice bran extract, fig extract, lotus seed extract, and yuzu extract in a weight ratio of 2:2:1:1.

When a skin external preparation according to one aspect contains rice bran extract, fig extract, lotus seed extract, and citron extract in a weight ratio of 10 to 1:10 to 1:1:1, a synergistic effect occurs to exhibit high DPPH radical scavenging activity, tyrosinase inhibitory effect, and melanin production inhibitory effect, thereby exhibiting significantly excellent antioxidant and whitening effects. When the respective extracts are mixed in a weight ratio out of the above range, the synergistic effect may be reduced or not appear, thereby exhibiting low antioxidant and whitening effects.

The above skin external preparation may be a cream, a gel, an ointment, a skin emulsifier, a skin suspension, a transdermal patch, a drug-containing bandage, a lotion, or a combination thereof. The above skin external preparation may be appropriately mixed with ingredients commonly used in skin external preparations such as cosmetics or medicines, such as aqueous ingredients, oily ingredients, powder ingredients, alcohols, moisturizers, thickeners, ultraviolet absorbers, whitening agents, preservatives, antioxidants, surfactants, fragrances, colorants, various skin nutrients, or a combination thereof, as needed. The above skin external preparation may also appropriately contain metal sequestrants such as sodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, and gluconic acid; caffeine, tannin, bellapamil, licorice extract, glablidine, hot water extract of the fruit of Kalin; various herbal medicines; drugs such as tocopheryl acetate, glycyrrhizic acid, tranexamic acid, and derivatives or salts thereof; and sugars such as vitamin C, magnesium ascorbic acid phosphate, ascorbic acid glucoside, arbutin, kojic acid, glucose, fructose, and trehalose.

Additionally, the above skin external agent may be included in an over-the-counter pharmaceutical composition.

The above term "quasi-drug" means a fiber, rubber product or similar product used for the purpose of treating, alleviating, managing or preventing diseases of humans or animals; a product that has a weak effect on the human body or does not directly affect the human body and is not an apparatus or machine and similar product; a product that is one of the preparations used for sterilization, insecticide and similar purposes to prevent infection, excluding products used for the purpose of diagnosing, treating, alleviating, managing or preventing the condition or disease of humans or animals that are not apparatuses, machines or devices; and products used for the purpose of exerting a pharmacological effect on the structure and function of humans or animals that are not apparatuses, machines or devices. It may include not only external skin preparations but also personal hygiene products.

A skin topical preparation containing rice bran extract, fig extract, lotus seed extract and citron extract according to the daily aspect exhibits significantly superior DPPH radical scavenging activity, tyrosinase inhibitory effect and melanin production inhibitory activity due to synergy effects compared to a single extract or a mixed extract of two or three extracts, and thus can exhibit superior antioxidant and skin whitening activities.

Another aspect provides a method for preparing a cosmetic composition comprising the step of mixing rice bran extract, fig extract, lotus seed extract and yuzu extract.

The above “rice bran”, “fig”, “alba water lily”, “yuzu”, “extract”, “skin whitening”, etc. may be within the above-mentioned scope.

In one specific example, the mixing step may be mixing rice bran extract, fig extract, lotus seed extract, and citron extract in a weight ratio of 10 to 1:10 to 1:1:1. Specifically, it may be mixing in a weight ratio of 6 to 1:6 to 1:1:1. For example, rice bran extract, fig extract, lotus seed extract, and yuzu extract may be mixed in a weight ratio of 6: 6: 1: 1, 6: 4: 1: 1, 6: 2: 1: 1, 6: 1: 1: 1, 4: 6: 1: 1, 4: 4: 1: 1, 4: 2: 1: 1, 4: 1: 1: 1, 2: 6: 1: 1, 2: 4: 1: 1: 1, 2: 2: 1: 1: 1, 2: 1: 1: 1, 1: 6: 1: 1, 1: 4: 1: 1, 1: 2: 1: 1: 1 or 1: 1: 1: 1. More specifically, rice bran extract, fig extract, lotus seed extract, and yuzu extract may be mixed in a weight ratio of 2:2:1:1.

In the above mixing step, when the rice bran extract, fig extract, lotus seed extract, and citron extract are mixed in a weight ratio of 10 to 1:10 to 1:1:1, a synergistic effect occurs to exhibit high DPPH radical scavenging activity, tyrosinase inhibition effect, and melanin production inhibition effect, thereby exhibiting significantly excellent antioxidant and whitening effects. In contrast, when the respective extracts are mixed in a weight ratio outside the above range, the synergistic effect may be reduced or not exhibited, thereby exhibiting low antioxidant and whitening effects.

In addition, a cosmetic composition manufactured by a manufacturing method according to one aspect exhibits significantly superior DPPH radical scavenging activity, tyrosinase inhibitory effect, and melanin production inhibitory activity due to a synergistic effect compared to a single extract or a mixed extract of two or three extracts, and thus can exhibit superior antioxidant and skin whitening activities.

A cosmetic composition containing rice bran extract, fig extract, Nymphaea Alba extract and citron extract according to one aspect has excellent radical scavenging ability, tyrosinase activity inhibition ability and melanin production inhibition ability, and therefore exhibits excellent effects on antioxidant and skin whitening. Therefore, the composition can be usefully utilized in cosmetics for antioxidant and skin whitening, external skin preparations, etc.

Hereinafter, the present invention will be described in more detail through examples. However, these examples are intended to exemplify the present invention and the scope of the present invention is not limited to these examples.

<Manufacturing Example>

Manufacturing Example 1: Manufacturing of rice bran extract

After grinding 100 g of dried rice bran, it was extracted under heating for 4 hours using 3 L of 70% EtOH, and then filtered through a filter paper at room temperature to obtain a filtrate. The obtained filtrate was concentrated under reduced pressure to obtain 200 mL of a 10-fold concentrated concentrate. The concentrate was recovered and dried with hot air, vacuum-dried, or freeze-dried to prepare a dry powder (8 g) of the extract. Thereafter, 1 g of the prepared dry powder was dissolved in 100 mL of 70% EtOH and used in the experiment.

Manufacturing Example 2: Manufacturing of Fig Extract

100 g of dried fig fruit (including peel, pulp, and seeds) was ground, and extracted under heating for 4 hours using 3 L of 70% EtOH, and then filtered through a filter paper at room temperature to obtain a filtrate. The obtained filtrate was concentrated under reduced pressure to obtain 200 mL of a 10-fold concentrated concentrate. The concentrate was recovered and subjected to a hot air drying, vacuum drying, or freeze drying process to prepare a dry powder (7 g) of the extract. Thereafter, 1 g of the prepared dry powder was dissolved in 100 mL of 70% EtOH and used in the experiment.

Manufacturing Example 3: Manufacturing of Alba Lotus Flower Extract

After grinding 100 g of dried lotus flowers, they were extracted under heating for 4 hours using 3 L of 70% EtOH, and then filtered through a filter paper at room temperature to obtain a filtrate. The obtained filtrate was concentrated under reduced pressure to obtain 200 mL of a 10-fold concentrated concentrate. The concentrate was recovered and dried with hot air, vacuum-dried, or freeze-dried to prepare a dry powder (8 g) of the extract. Thereafter, 1 g of the prepared dry powder was dissolved in 100 mL of 70% EtOH and used in the experiment.

Manufacturing Example 4: Manufacturing of Yuja Extract

100 g of dried citron fruit (including peel, pulp, and seeds) was ground, and then extracted under heating for 4 hours using 3 L of 70% EtOH, and then filtered through a filter paper at room temperature to obtain a filtrate. The obtained filtrate was concentrated under reduced pressure to obtain 200 mL of a 10-fold concentrated concentrate. The concentrate was recovered and subjected to a hot air drying, vacuum drying, or freeze drying process to prepare a dry powder (7 g) of the extract. Thereafter, 1 g of the prepared dry powder was dissolved in 100 mL of 70% EtOH and used in the experiment.

<Example> Preparation of mixed extract containing rice bran extract, fig extract, lotus flower extract and yuzu extract

The mixed extracts including the rice bran extract, fig extract, lotus flower extract, and citron extract prepared in the above Manufacturing Examples 1 to 4 were mixed in the composition shown in Table 1 below to prepare a mixed extract. Specifically, after mixing each extract in the composition shown in Table 1 below, a total of 20 g of the mixed extract was prepared, and the mixed extract was transferred to a sterilized container (KRS A-1, Health up, Korea) and stored in the refrigerator at 5°C.

In addition, in order to confirm the activity of a composition lacking some ingredients without including all of the extracts and ingredients, the compositions of Comparative Examples 1 to 9 in Table 1 below were also prepared in the same 20 g amount. In Table 1 below, the weight ratio of rice bran, fig, lotus flower, and citron refers to the weight ratio of the extracts mixed with the ethanol aqueous solution.

division Weight ratio Rice bran fig Alba training flower Yuzu Example 1 2 2 1 1 Example 2 2 1 1 1 Example 3 1 2 1 1 Example 4 1 1 2 1 Example 5 1 1 1 2 Example 6 1 1 1 1 Comparative Example 1 1 - - - Comparative Example 2 - 1 - - Comparative Example 3 - - 1 Comparative Example 4 - - - 1 Comparative Example 5 - - 1 1 Comparative Example 6 1 - 1 1 Comparative Example 7 - 1 1 1 Comparative Example 8 1 1 1 - Comparative Example 9 1 1 - 1

<Experimental example>

Experimental Example 1: Confirmation of antioxidant capacity (DPPH assay)

In order to confirm the antioxidant activity of rice bran extract, fig extract, lotus flower extract, and yuzu extract, DPPH radical scavenging activity was evaluated for Examples 1 to 6 and Comparative Examples 1 to 9.

Specifically, 100 μl of 0.1 mM DPPH solution was added to 50 μl of the mixed extract at a concentration of 50 μg/ml, and the reaction was performed for about 10 minutes at room temperature in a dark place, and the absorbance was measured at 517 nm.

Alpha-tocopherol was used as a control, and the DPPH radical scavenging activity was calculated using the following mathematical formula 1:

[Mathematical formula 1]

DPPH radical scavenging activity (%) = {1 - OD _sample / OD _control } X 100.

division DPPH radical scavenging activity (%) Example 1 67.4 Example 2 63.7 Example 3 62.6 Example 4 57.9 Example 5 56.3 Example 6 53.9 Comparative Example 1 36.4 Comparative Example 2 35.6 Comparative Example 3 33.0 Comparative Example 4 31.2 Comparative Example 5 38.8 Comparative Example 6 42.8 Comparative Example 7 42.5 Comparative Example 8 46.9 Comparative Example 9 46.7

As shown in Table 2 above, Examples 1 to 6 exhibited significantly superior DPPH radical scavenging activity than Comparative Examples 1 to 9.

Furthermore, the DPPH radical scavenging activity of Examples 1 to 3 was high, and in particular, Example 1, which was a mixed extract in which rice bran extract, fig extract, lotus flower extract, and citron extract were mixed in a ratio of 2: 2: 1: 1, exhibited the highest DPPH radical scavenging activity.

That is, in the case of a mixed extract containing rice bran extract, fig extract, water lily flower extract, and citron extract, it exhibited significantly superior antioxidant activity than when the extracts were used as a single extract or in combination of only two or three, and in particular, when rice bran extract, fig extract, water lily flower extract, and citron extract were combined in a weight ratio of 2: 2: 1: 1, it was confirmed that it exhibited high antioxidant activity, exhibiting an excellent skin whitening effect.

Experimental Example 2: Confirmation of Tyrosinase Inhibitory Activity

In order to confirm the whitening effect of rice bran extract, fig extract, lotus flower extract and citron extract, tyrosinase inhibitory activity was evaluated for Examples 1 to 6 and Comparative Examples 1 to 9.

Specifically, 50 μl of 1.5 mM L-tyrosine was added to 150 μl of 0.1 M phosphate buffer solution at pH 6.8. Thereafter, Examples 1 to 6 and Comparative Examples 1 to 7 were added at concentrations of 50 μg/ml and 500 μg/ml. Thereafter, 30 μl of tyrosinase (100 U/ml) was added and reacted at 37°C for 15 minutes. DMSO was used as a control, and 0.1 M phosphate buffer solution was added instead of tyrosinase enzyme as a blank sample solution and reacted in the same manner as above. Thereafter, the absorbance was measured at 490 nm and the tyrosinase inhibitory effect was calculated by the following mathematical formula 2:

[Mathematical formula 2]

Tyrosinase inhibition effect (%) = {1 - OD _sample / OD _control } X 100.

division Tyrosinase inhibitory activity (%) 50 μg/ml 500 ㎍/ml Example 1 53.4 67.8 Example 2 49.0 62.7 Example 3 46.7 59.6 Example 4 41.2 53.1 Example 5 40.9 52.3 Example 6 40.1 51.7 Comparative Example 1 20.1 29.6 Comparative Example 2 19.2 28.1 Comparative Example 3 14.8 26.2 Comparative Example 4 12.1 25.6 Comparative Example 5 23.3 31.5 Comparative Example 6 29.5 39.2 Comparative Example 7 28.9 38.8 Comparative Example 8 34.6 46.7 Comparative Example 9 34.0 46.5

As shown in Table 3 above, Examples 1 to 6 exhibited significantly superior tyrosinase inhibitory activity compared to Comparative Examples 1 to 9 at both concentrations of 50 μg/ml and 500 μg/ml.

Furthermore, the tyrosinase inhibitory activity of Examples 1 to 3 was high, and in particular, the highest tyrosinase inhibitory activity was exhibited in Example 1, which was a mixed extract in which rice bran extract, fig extract, lotus flower extract, and citron extract were mixed in a ratio of 2: 2: 1: 1.

That is, in the case of a mixed extract containing rice bran extract, fig extract, water lily flower extract, and citron extract, it exhibited significantly better tyrosinase inhibition activity, i.e., a whitening effect, than when the extracts were used as a single extract or in combination of only two or three, and in particular, when rice bran extract, fig extract, water lily flower extract, and citron extract were combined in a weight ratio of 2: 2: 1: 1, it was confirmed that it exhibited high tyrosinase inhibition activity, exhibiting an excellent skin whitening effect.

Experimental Example 3: Inhibitory activity of melanin production in cells

In order to confirm the whitening effect of rice bran extract, fig extract, lotus flower extract and citron extract, the melanin production inhibition activity was evaluated for Examples 1 to 6 and Comparative Examples 1 to 9.

Specifically, mouse melanoma cells, B16-F10 melanoma cells (ATCC), were seeded at a density of 2 X 10 ⁴ cells/well in a 24-well plate and cultured for 24 hours. Then, to induce melanin production, new medium containing 100 nM α-melanocyte stimulating hormone (MSH) and test substances was treated and cultured for 72 hours. After 72 hours of culture, the culture medium was removed, washed with 500 ㎕ of DPBS, and then removed again. Then, 110 ㎕ of 1 N NaOH (10% DMSO) was added to each well and cultured at 60 ℃ for 1 hour to dissolve. After mixing the culture medium, 90 ㎕ was dispensed into another 96-well plate and the absorbance was measured at 490 nm together with a melanin standard substance. The amount of melanin produced was calculated by the following mathematical formula 3:

[Mathematical formula 3]

Melanin production inhibition effect (%) = {1 - OD _sample / OD _control } X 100.

division Melanin production inhibition effect (%) Example 1 37.6 Example 2 31.4 Example 3 30.9 Example 4 24.9 Example 5 24.7 Example 6 23.1 Comparative Example 1 10.1 Comparative Example 2 9.8 Comparative Example 3 6.0 Comparative Example 4 5.7 Comparative Example 5 12.2 Comparative Example 6 14.9 Comparative Example 7 14.6 Comparative Example 8 18.0 Comparative Example 9 17.9

As shown in Table 4 above, Examples 1 to 6 exhibited significantly superior melanin production inhibition effects compared to Comparative Examples 1 to 9.

Furthermore, the melanin production inhibition effect of Examples 1 to 3 was high, and in particular, the highest melanin production inhibition effect was shown in Example 1, which was a mixed extract in which rice bran extract, fig extract, lotus flower extract, and citron extract were mixed in a ratio of 2: 2: 1: 1.

That is, in the case of a mixed extract containing rice bran extract, fig extract, water lily flower extract, and citron extract, it exhibited a significantly better melanin production inhibition effect, i.e., whitening effect, than when the extracts were used as a single extract or combined in only two or three, and in particular, when rice bran extract, fig extract, water lily flower extract, and citron extract were combined in a weight ratio of 2: 2: 1: 1, it was confirmed that it exhibited a high melanin production inhibition effect, thereby exhibiting an excellent skin whitening effect.

<Formulation example>

Formulation Example 1. Preparation of whitening moisturizing cream

A whitening moisturizing cream was prepared using the mixed extract of Example 1, which showed the best antioxidant activity, tyrosinase inhibitory activity, and melanin production inhibition effect, i.e., skin whitening effect, in Experimental Examples 1 to 3 above.

Specifically, it was manufactured by a conventional method according to the ingredients and composition ratio shown in Table 5 below.

ingredient Content (weight%) Rice Bran Extract 0.1 Fig extract 0.1 Alba water lily flower extract 0.05 Yuzu extract 0.05 Disodium EDTA a very small amount glycerin 6.0 Polyglyceryl-3 Methylglucose Distearate 1.0 Cetyl Ethylhexanoate 3.0 Dimethicone 0.5 Carbomer a very small amount Arginine a very small amount 1,2-Hexanediol 2.0 Purified water To 100

Formulation Example 2. Preparation of Whitening Nutrition Serum

A whitening nutrient serum was prepared using the mixed extracts of Examples 1 to 3 above, which showed the best antioxidant activity, tyrosinase inhibitory activity, and melanin production inhibition effect, i.e., skin whitening effect.

Specifically, it was manufactured using a conventional method according to the ingredients and composition ratio shown in Table 6 below.

ingredient Content (weight%) Rice Bran Extract 0.1 Fig extract 0.1 Alba water lily flower extract 0.05 Yuzu extract 0.05 glycerin 0.1 Hydroxyethyl cellulose (Hydroxymethyl Cellulose) a very small amount Sodium Citrate a very small amount Beeswax 0.5 Polysorbate 80 1.5 Carbomer a very small amount Arginine a very small amount alcohol 5.0 PEG-40 Hydrogenated Castor Oil 0.4 1,2-Hexanediol 2.0 Purified water To 100

Claims (12)

An antioxidant or skin whitening cosmetic composition comprising rice bran extract, fig extract, Nymphaea Alba extract, and yuzu extract.
A cosmetic composition according to claim 1, wherein the Alba water lily is at least one selected from the group consisting of buds, roots, stems, branches, leaves, whole plants, bark, flowers, fruits, and seeds of Alba water lily.
A cosmetic composition according to claim 1, wherein the extract is extracted with water, an alcohol having 1 to 5 carbon atoms, hexane, chloroform, butylene glycol, or a mixed solvent thereof.
A cosmetic composition according to claim 1, wherein the composition comprises rice bran extract, fig extract, lotus seed extract, and citron extract in a weight ratio of 10 to 1:10 to 1:1:1.
delete A cosmetic composition according to claim 1, wherein the antioxidant effect is by radical scavenging.
A cosmetic composition according to claim 1, wherein the skin whitening is achieved by at least one agent selected from the group consisting of inhibition of tyrosinase activity and inhibition of melanin production.
A cosmetic composition according to claim 1, wherein the skin whitening alleviates at least one selected from the group consisting of freckles, liver spots, lentigines, moles, café au lait spots, Ota's nevus, blue nevus, hyperpigmentation spots, hyperpigmentation after drug use, gravidic chloasma, and post-inflammatory hyperpigmentation due to wounds or dermatitis.
A cosmetic composition according to claim 1, wherein the cosmetic composition is in a formulation selected from the group consisting of serum, toner, essence, paste, mask pack, patch, gel, cream, lotion, nutritional lotion, nutritional cream, moisturizing cream, massage cream, powder, soap, cleanser, oil, foundation, makeup base, wax, and spray.
An antioxidant or skin whitening external skin preparation containing rice bran extract, fig extract, lotus seed extract and yuzu extract.
A method for producing an antioxidant or skin whitening cosmetic composition comprising a step of mixing rice bran extract, fig extract, lotus seed extract and yuzu extract.
A manufacturing method according to claim 11, wherein the mixing step comprises mixing rice bran extract, fig extract, lotus seed extract, and citron extract in a weight ratio of 10 to 1:10 to 1:1:1.