KR102844606B1 - Cosmetic composition containing sialyllactose for soothing skin, moisturizing skin, alleviating itching, and improving skin barrier - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising 3'-sialyllactose and its salts, 6'-sialyllactose and its salts, bisabolol and glyceryl glucoside. The composition of the present invention has been confirmed to have skin barrier strengthening, skin cell differentiation and regeneration promotion, skin elasticity strengthening, skin immune balance maintenance, skin soothing effect, skin moisturizing effect increase, itching relief effect and anti-inflammatory effect, and thus can be usefully used as a raw material for a cosmetic composition.

Description

Cosmetic composition containing sialyllactose for soothing skin, moisturizing skin, alleviating itching, and improving skin barrier

The present invention relates to providing a cosmetic composition for skin soothing, skin moisturizing, alleviating itching and improving the skin barrier, comprising sialyllactose, bisabolol and glyceryl glucoside, which is effective in improving the skin barrier by increasing the expression of genes involved in the skin barrier and is effective in moisturizing the skin by activating the aquaporin (AQP3) gene.

The skin, located at the outermost layer of the human body, functions as a barrier to protect the body from external environmental factors such as dry air, ultraviolet rays, microorganisms, and chemical and physical stimuli. Skin tissue is largely composed of three layers: the epidermis, the dermis, and the subcutis. The stratum corneum, the outermost layer of the epidermis, is composed of keratinocytes and skin lipids. Among the skin lipids, keratinocytes differentiate and produce lipids that fill the spaces between keratinocytes, providing intercellular cohesion and acting as a skin barrier. Furthermore, the stratum corneum contains a hydrophilic substance called natural moisturizing factor, which has the ability to retain moisture, contributing to skin hydration. Furthermore, the appropriate amount of oil formed by sebum from the sebaceous glands and sweat from the sweat glands minimizes moisture loss and maintains skin hydration.

The normal moisture content of the stratum corneum is 10 to 20%. If the moisture content falls below 10%, the skin becomes dry and loses its protective function, leading to signs of aging. Therefore, maintaining moisture in the stratum corneum is essential for maintaining healthy skin.

However, even healthy skin that is well-moisturized can become dry, dull, and rough due to various factors such as stress from environmental pollution in modern society, changes in the harsh external environment such as wind, extreme cold, and ultraviolet rays, changes in social life such as temperature control such as air conditioning and heating, natural aging due to aging, and skin stress from makeup habits. Therefore, moisturizers have become an essential element. In addition, research is actively being conducted to improve skin tightness, increase moisturizing power, and increase dermal density to achieve healthier skin.

Meanwhile, sialyllactose (3'- & 6'-sialyllactose) is a representative HMO (Human Milk Oligosaccharide) component contained in breast milk, and is known as an immune-activating substance with excellent antibacterial and antiviral functions. It is a core component of brain structures and plays an important role in the development of infant brain function, and is also known to have prebiotic effects for improving intestinal function. In addition, Korean Patent No. 10-1773066 describes the effect of sialyllactose on the treatment of degenerative arthritis. However, there is no known technology for simultaneously containing 3'-sialyllactose and 6'-sialyllactose and using bisabolol and glyceryl glucoside, and there is a need for the development of substances that have various effects such as improving skin moisturization and skin inflammation in addition to skin whitening.

Accordingly, the inventors of the present invention have developed a cosmetic composition that is effective in improving the skin barrier by increasing the expression of genes involved in the skin barrier, including sialyllactose, bisabolol, and glyceryl glucoside, and that is effective in moisturizing the skin by activating the aquaporin (AQP3) gene, which is a moisture channel within skin cells, and filling deep moisture at the cellular level.

The problem to be solved by the present invention is to provide a cosmetic composition having skin barrier strengthening, skin cell differentiation and regeneration promotion, skin elasticity strengthening, skin immune balance maintenance, skin moisturizing, itching relief, and anti-inflammatory effects, including 3'-sialyllactose and its salts, 6'-sialyllactose and its salts, bisabolol, and glyceryl glucoside.

The problems to be solved by the present invention are not limited to the problems mentioned above, and other technical problems not mentioned can be clearly understood by a person having ordinary skill in the technical field to which the present invention belongs from the description below.

In order to solve the above problem, according to one aspect of the present invention, a cosmetic composition for skin soothing, skin moisturizing, itching relief, and skin barrier improvement containing sialyllactose is provided.

The cosmetic composition comprising 3'-sialyllactose and its salts, 6'-sialyllactose and its salts, bisabolol and glyceryl glucoside of the present invention has effects such as strengthening the skin barrier, promoting skin cell differentiation and regeneration, enhancing skin elasticity, maintaining skin immune balance, soothing effect, increasing moisturizing power, alleviating itching and anti-inflammation.

The effects of the present invention are not limited to the effects described above, and should be understood to include all effects that can be inferred from the composition of the invention described in the description or claims of the present invention.

Figure 1 is a drawing confirming that the sialyl lactose of the present invention increases the expression of CLDN-1 and TGM genes, which are genes involved in the skin barrier.
Figure 2 is a drawing confirming that the sialyl lactose of the present invention increases the expression of IVL and KRT-1 genes, which are genes involved in skin structure and differentiation.
Figure 3 is a drawing confirming the ability of 3'-sialyllactose of the present invention to promote skin cell collagen synthesis.
Figure 4 is a drawing confirming that the sialyllactose of the present invention increases aquaporin (AQP3) gene expression.
Figure 5 is a drawing confirming that the sialyl lactose of the present invention maintains the balance of skin immunity.
Figure 6 is a drawing confirming that the 3'-sialyllactose of the present invention reduces the expression of an inflammation-suppressing related gene (Cox-2).
Figure 7 is a graph showing the results of measuring the skin surface moisture content of the cosmetic composition of the present invention.
Figure 8 is a graph showing the results of measuring the increase rate of skin moisturizing power of the cosmetic composition of the present invention.
Figure 9 is a graph showing the results of measuring the transepidermal water loss (TEWL, g/m ² /hr) of the cosmetic composition of the present invention.

The present invention is described in detail below.

The present invention relates to a cosmetic composition for improving the skin barrier and alleviating itching or moisturizing, comprising 0.01 to 10 wt% of a compound represented by sialyllactose and its salts, 0.01 to 5 wt% of bisabolol, and 0.01 to 10 wt% of glyceryl glucoside.

The above sialyllactose may be 3'-sialyllactose and its salts, 6'-sialyllactose and its salts.

The 3'-Sialyllactose Sodium Salt (CAS No. 128596-80-5) and 6'-Sialyllactose Sodium Salt (CAS No. 157574-76-0) of the present invention may be compounds having structures represented by Chemical Formula 1 and Chemical Formula 2, respectively.

(Chemical formula 1)

(Chemical formula 2)

The composition may further include at least one skin soothing ingredient selected from the group consisting of Centella Asiatica Extract, Madecassoside, Madecassic Acid, Asiaticoside, Asiatic Acid, Bisabolol, Allantoin, Beta-glucan, Sizophyllan, Cyanocobalamin, Alloperon, and metal complexes thereof, preferably bisabolol, but not limited thereto.

The above-mentioned bisabolol has previously been used as a fragrance ingredient in consumer products such as premium air fresheners, shampoos, soaps, and cosmetics. Bisabolol has also been associated with anti-inflammatory properties and has previously been included in compositions as an anti-inflammatory agent. Bisabolol may be of natural or synthetic origin, or may include a mixture of natural and synthetic origins. Bisabolol may be added to a cosmetic composition, for example, in pure form, as a salt, as an extract, or in any other form. Bisabolol includes, for example, "alpha-bisabolol," including (+)-alpha-bisabolol, (-)-alpha-bisabolol, (+)-epi-alpha-bisabolol, (-)-epi-alpha-bisabolol, and combinations thereof. The cosmetic composition may include bisabolol in an amount of 0.01% to 5% by weight of the cosmetic composition.

Bisabolol may have the following chemical formula:

(Chemical formula 3)

The composition may further include at least one skin moisturizing ingredient selected from the group consisting of hyaluronic acid and its salts, glyceryl glucoside, panthenol, xylityl glucoside, ectoin, glycerol, ethylene glycol, polypropylene glycol, diethylene glycol, butylene glycol, triethylene glycol, polyethylene glycol, sorbitol, and glycerin, preferably glyceryl glucoside, but is not limited thereto.

The above glyceryl glucoside is a substance related to the activation of Aquaporins (AQPs), which are moisture channels, and is known as an ingredient that activates AQP3, an excellent moisture channel in the epidermis among the 13 AQPs currently known. When the above glyceryl glucoside is included, the moisture hydration ability can be further improved. In addition, it has a low molecular weight, so it can easily pass through the skin cell wall, and it has an excellent feeling of use, giving a light and refreshing feeling without stickiness when applied to the skin. In addition, it has excellent freezing preservation ability and safety, so it has excellent self-preservative power and can be excellent in skin compatibility.

In one specific example, the glyceryl glucoside may be included in an amount of 0.01 to 10 wt% based on the total weight of the cosmetic composition. When included within this range, the formulation stability is not compromised, while the usability and moisturizing effect can be excellent. For example, the glyceryl glucoside may be included in an amount of 1 to 3 wt%.

According to the present invention, the cosmetic composition may enhance the expression of a gene selected from the group consisting of AQP-3 (Aquaporin-3), CLDN-1 (claudin-1), TGM-1 (transglutaminase-1), KRT-1 (keratin-1), and IVL (involucrin).

The above CLDN-1 (claudin-1) and TGM-1 (transglutaminase-1) are genes involved in the skin barrier. When the expression of the above genes increases, the cohesiveness of skin cells increases, which prevents the skin barrier from becoming loose.

As shown in Fig. 1, the 3'-sialyllactose and 6'-sialyllactose are effective in improving the skin barrier by increasing the expression of the CLDN-1 (claudin-1) and TGM-1 (transglutaminase-1) genes.

The above KRT-1 (keratin-1) and IVL (involucrin) genes are genes that function in the granular layer of the skin, and when the expression of the genes increases, the differentiation of skin cells is promoted, which helps skin regeneration.

As shown in Fig. 2, the 3'-sialyllactose and 6'-sialyllactose are effective in skin regeneration by increasing the expression of KRT-1 (keratin-1) and IVL (involucrin) genes.

In addition, as shown in Fig. 3, in a test to detect procollagen mRNA present in cells, procollagen in the medium secreted from cells was detected using a specific antibody specific for the C-terminal peptide (PIP) of human procollagen type I. Therefore, sialyllactose increases the expression of genes involved in collagen synthesis by more than two-fold, and as a result, collagen also increases, which is effective in improving wrinkles.

The above AQP-3 (Aquaporin-3) is a water channel within skin cells that transports water molecules inside and outside the cells. When the above gene is activated, the water content within the cells increases.

As shown in Fig. 4, the 3'-sialyllactose and 6'-sialyllactose increase the expression of the AQP-3 (Aquaporin-3) gene, thereby increasing the moisture content in the skin, i.e., increasing the skin's moisturizing power.

In addition, according to the present invention, the cosmetic composition is characterized by inhibiting the expression of a gene selected from the group consisting of IL-6 (Interleukin 6), Cox-2 (Cyclooxygenase-2), IL-1β (Interleukin-1β), and TNF-α (Tumor necrosis factor-α), which are inflammatory inducers.

When the skin's immune balance is disrupted and immune cells become overly activated, an inflammatory response is induced, which can cause various skin problems.

As shown in Fig. 5, when ConA is treated to create conditions in which Th2 is overly activated, INFγ decreases and IL-4 increases. 3'-sialyllactose and 6'-sialyllactose regulate this condition, thereby maintaining the Th1/Th2 immune balance and thus maintaining the balance of skin immunity.

Looking at Figure 6, after UV irradiation to keratinocytes to induce inflammation, 3'-sialyllactose treatment confirmed a decrease in the expression of Cox-2, an inflammation-inducing gene, and it was confirmed that the sialyllactose has an anti-inflammatory effect that suppresses excessive inflammatory response.

The cosmetic composition of the present invention is characterized by having a skin irritation relief effect, and the skin irritation is characterized by erythema or itching.

The cosmetic composition according to the present invention can be manufactured in the form of a liquid, gel, emulsion, cream, foam, toner, pack, softener, milk, foundation, makeup base, essence, soap, liquid cleanser, bath agent, sunscreen cream, and sun oil, and as a preferred embodiment, it can provide a softener, astringent, nourishing toner, gel, nourishing cream, massage cream, eye cream, eye essence, essence, cleansing cream, cleansing lotion, cleansing foam, cleansing water, pack, body lotion, body cream, body essence, patch, face mask, spray, and/or skin adhesive type cosmetic.

Hereinafter, the structure and operation of the present invention will be described in more detail through preferred embodiments of the present invention. However, these are presented as preferred examples of the present invention and should not be construed as limiting the present invention in any way.

Materials and Methods

Method for preparing a cosmetic composition

First, the water phase and the oil phase are each dissolved by double boiling at 70 to 75°C, and then the two dissolved phases are mixed and emulsified using an AGI-Mixer or Homo-Mixer. The emulsified phase is cooled to 50°C, and then the additive phase is added and cooled to 25°C to prepare a cosmetic composition for improving the skin barrier or moisturizing.

Experimental Example 1. Measurement of skin moisture content (%) and skin moisture increase rate (change rate) of the composition

The assessment of stratum corneum hydration, also known as skin moisture content, primarily utilizes the electrical properties of the stratum corneum. The most commonly used method for assessing skin surface moisture is capacitance. Subjects were selected, cosmetics were applied to the forearm, and capacitance was measured over time.

award Ingredients Comparative Example 1 Example 1 Example 2 Example 3 Example 4 premier purified water To 100 To 100 To 100 To 100 To 100 glycerin 3 3 3 3 3 Disodium EDTA 0.02 0.02 0.02 0.02 0.02 antiseptic Appropriate amount Appropriate amount Appropriate amount Appropriate amount Appropriate amount xanthan gum 0.02 0.02 0.02 0.02 0.02 Carbomer 0.1 0.1 0.1 0.1 0.1 Tromethamine 0.08 0.08 0.08 0.08 0.08 Paid Caprylic/Capric Triglyceride 3 3 3 3 3 Cetearyl alcohol 0.2 0.2 0.2 0.2 0.2 Cetearyl Olivate & Sorbitan Olivate 1 1 1 1 1 Additional prize Sialyllactose - 1 - - 1 Bisabolol - - 0.5 - 0.5 Glyceryl glucoside - - - 3 3 total 100 100 100 100 100

Composition
Time (hr) Mudopo Comparative Example 1 Example 1 Example 2 Example 3 Example 4 0 32.8 33.6 31.7 32.7 33.3 32.0 1 33.9 35.8 37.8 36.4 37.5 38.0 2 34.1 36.4 37.7 35.6 37.2 37.9 3 33.7 36.9 37.8 36.9 38.0 38.3

Compositions were prepared with the compositions and contents shown in Table 1 above, and the skin moisture content was measured after applying the prepared composition to the skin to confirm the skin moisture enhancement effect. The skin surface moisture content (%) was measured by applying no application, Comparative Example 1, and Examples 1 to 4 to each section. The moisture content was measured using a Moisture Checker (Scalar MY-808S, Japan). Six 2 cm x 2 cm squares were drawn on the inner arms of five test subjects, and the moisture content was measured. After applying 0.1 g of the composition to each section and allowing sufficient absorption, the moisture content was measured using a moisture meter before application and after 1, 2, and 3 hours. The experiment was conducted in a constant temperature and humidity room with an indoor temperature of 25±2℃ and a relative humidity of 55±5%. The moisturizing power increase rate calculation formula is as follows.

[Calculation formula]

Moisturizing power increase rate (%) = Moisturizing power before application - Moisturizing power 1 to 3 hours after sample application

Composition
Time (hr) Mudopo Comparative Example 1 Example 1 Example 2 Example 3 Example 4 0 0 0 0 0 0 0 1 1.1 2.2 6.1 3.7 4.2 6 2 1.3 2.8 6 3.5 3.9 5.9 3 0.9 3.3 6.1 4.2 4.7 6.3

Experimental Example 2. Transepidermal water loss (TEWL, g/m) of the composition ² /hr) measurement

The skin barrier (transepidermal water loss) was measured using the GPSkin Barrier (G-Power Co., Ltd.). The average values of the skin barrier test measured 1 to 3 hours after using Comparative Example 1 and Examples 1 to 4 are shown in Table 4 below.

experimental group
Time (hr) Mudopo Comparative Example 1 Example 1 Example 2 Example 3 Example 4 0 12.0 12.5 16.5 13.5 14.0 13.5 1 6.0 5.0 7.5 5.5 5.5 5.0 2 4.0 5.0 3.5 4.5 4.5 3.5 3 2.5 4.5 3.0 3.5 2.0 1.5

From the results in Tables 2 to 4 above, the composition of the present invention achieved the best values for skin surface moisture content and moisture increase rate, which indicate a skin moisturizing effect, in Example 4 (see Figures 7 to 8). In terms of transepidermal water loss, which indicates skin barrier function, Example 4 also showed the lowest loss even after 3 hours (see Figure 9).

Experimental Example 3. Measurement of the skin soothing effect of the composition

1) Cosmetic composition formulation example: Preparation of cream

Sialyllactose 1.0 wt%, bisabolol 0.5 wt%, glyceryl glucoside 2.0 wt%, dipropylene glycol 1 to 10 wt%, caprylic/capric triglyceride 1 to 10 wt%, hydrogenated polydecene 1 to 10 wt%, squalane 1 to 10 wt%, butylene glycol 1 to 10 wt%, cetearyl olivate 0.1 to 10 wt%, cetearyl alcohol 0.1 to 10 wt%, hydrogenated vegetable oil 0.1 to 10 wt%, sorbitan olivate 0.1 to 10 wt%, cocos nucifera (coconut) oil 0.1 to 10 wt%, panthenol 0.1 to 10 wt%, vinyl dimethicone 0.1 to 10 % by weight, hydroxyethyl urea 0.1 to 10 wt%, simethicone 0.1 to 10 wt%, hydroxypropyl bispalmitamide MEA 0.1 to 10 wt%, hydroxyacetophenone 0.1 to 10 wt%, glyceryl stearate 0.1 to 5 wt%, hydrogenated olive oil unsaponifiables 0.1 to 5 wt%, potassium cetyl phosphate 0.1 to 5 wt%, glycerin 0.01 to 5 wt%, carbomer 0.01 to 2 wt%, tromethamine 0.01 to 2 wt%, tocopherol 0.01 to 2 wt%, 1,2-hexanediol 0.01 to 2 wt%, disodium EDTA 0.001 to 1 wt%, xanthan gum A cream was prepared using a conventional method with the contents of 0.001 to 1 wt% of sodium hyaluronate, 0.001 to 1 wt% of pentylene glycol, 0.001 to 1 wt% of sizophyllan, and the remainder of purified water.

2) Method for evaluating skin soothing effect

The skin soothing effect was evaluated by conducting a human patch test using the cream manufactured in the cosmetic composition formulation example.

For this purpose, 20 adult female subjects aged 20 to 59 years were tested. After using the product for 2 weeks (twice a day), the test area was washed and the skin was allowed to rest for 30 minutes before being evaluated.

The skin soothing effect was measured using VISIA-CR (Canfield Scientific Inc., USA) to photograph the subjects' faces. VISIA-CR is a high-resolution photography device with six built-in flashes that takes pictures by setting each combination of lighting. The images taken with VISIA-CR were analyzed using the image analysis program I-MAX Plus (ING plus Co., Ltd., Korea), and the a* value, which indicates redness within the designated area of the cheek area of the face, was analyzed. As the a* value decreases, redness decreases, indicating a skin soothing effect.

3) Skin soothing effect measurement results (a*)

Raw data (Mean±SD Before use 9.456±2.603 After 2 weeks of use 8.905±2.030 Change rate (%) Before use - After 2 weeks of use -4.383 Improved subjects (n/%) Before use - After 2 weeks of use 17 / 85,000 Statistical analysis (p-value) Before use - After 2 weeks of use 0.012*

*: p<0.05 by Paired T-Test.

According to the above results, the cosmetic composition according to the example showed a significant decrease of 4.383% (p<0.05) from 9.456 before use to 8.905 after two weeks of use (Table 5). Therefore, it was confirmed that the cosmetic composition was effective in soothing the skin.

Experimental Example 4. Skin Irritation (Itching) Evaluation

Experimental Example 3. The skin irritation (itching) effect was evaluated by conducting a human patch test using the cream manufactured in the cosmetic composition formulation example.

As a result of measuring the Visual Analogue Scale (VAS) to evaluate itchiness, the VAS value in the test group significantly decreased by 42.963% from 6.956 before use to 4.082 after 2 weeks of use (p<0.05) and significantly decreased by 71.762% to 2.000 after 4 weeks of use. On the other hand, in the control group, the VAS value significantly increased by 2.498% from 6.879 before use to 7.029 after 2 weeks of use (p<0.05) and significantly increased by 4.176% to 7.121 after 4 weeks of use (p<0.05). As a result of comparing the test group and the control group, a statistically significant difference (p<0.05) was observed in both the measurement sections after 2 weeks of use and after 4 weeks of use (see Table 6).

Raw data (Mean±SD) test group control group Before use 6.956±1.432 6.879±1.360 After 2 weeks of use 4.082±1.629 7.029±1.312 After 4 weeks of use 2.000±1.343 7.121±1.266 Change rate (%) test group control group Before use - After 2 weeks of use -42.963 2.489 Before use - After 4 weeks of use -71.762 4.176 Improved test subjects (n/%) test group control group Before use - After 2 weeks of use 34/100,000 3/8.824 Before use - After 4 weeks of use 34/100,000 7/20.588 Statistical analysis (p-value) test group control group In the military
comparison within-subject test effect < 0.001* 0.001* Before use - After 2 weeks of use < 0.001* < 0.001* Before use - After 4 weeks of use < 0.001* 0.001* After 2 weeks of use < 0.001** After 4 weeks of use < 0.001** Preliminary homogeneity test 0.822

Abbreviations: SD, Standard Deviation. *: p<0.05 by Repeated Measures ANOVA, post hoc Bonferroni correction.

**: p<0.05 by Independent T-Test

As shown in the results in Table 6, it can be confirmed that the cosmetic containing sialyllactose, bisabolol, and glyceryl glucoside showed a significant anti-itching effect after 2 weeks and 4 weeks of use compared to the test group.

Statistical analysis

The primary efficacy evaluation variables were evaluated based on the analysis values of itching evaluation scores, transepidermal water loss, and moisture content, and the secondary efficacy evaluation variables were evaluated based on the results of the subject satisfaction questionnaire after using the test product.

The analysis target group was the PP group (Per Protocol Set).

In the statistical method, the statistical analysis program was used as the SPSS Statistics 27 Standard program, and significance was determined when p<0.05 at the 95% confidence interval. When the number of subjects exceeded 30, normality was assumed according to the central limit theorem (C.L.T) and the analysis was performed parametrically. Repeated measures ANOVA was used for data with repeated measurements three or more times, and post hoc tests were performed using the Bonferroni method. Between-group comparisons were performed by calculating the change from before use in each of the test and control groups and using the independent t-test. The homogeneity test was performed by comparing the raw data before use in the test and control groups and using the independent t-test.

The foregoing description of the present invention is for illustrative purposes only, and those skilled in the art will readily appreciate that the present invention can be readily modified into other specific forms without altering the technical spirit or essential characteristics of the present invention. Therefore, the embodiments described above should be understood as illustrative in all respects and not restrictive. For example, each component described as a single entity may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined manner.

The scope of the present invention is indicated by the claims set forth below, and all changes or modifications derived from the meaning and scope of the claims and their equivalent concepts should be interpreted as being included in the scope of the present invention.

Claims (11)

Contains Sialyllactose, Bisabolol, and Glyceryl Glucoside.
A cosmetic composition for skin soothing, skin moisturizing, alleviating itching and improving skin barrier, comprising 0.1 to 2.0 wt% of the above sialyllactose, 0.1 to 2.0 wt% of the above bisabolol, and 0.5 to 5.0 wt% of the above glyceryl glucoside.
In the first paragraph,
A cosmetic composition for skin soothing, skin moisturizing, itching relief and skin barrier improvement, characterized in that the above sialyllactose is 3'-sialyllactose and its salts or 6'-sialyllactose and its salts.
delete delete delete delete In the first paragraph,
A cosmetic composition for skin soothing, skin moisturizing, alleviating itching and improving the skin barrier, characterized in that the cosmetic composition enhances the expression of any one gene selected from the group consisting of CLDN-1 (Claudin-1), TGM-1 (Transglutaminase-1), KRT-1 (Keratin-1), IVL (Involucrin), PCOLCE (Procollagen C-endopeptidase enhancer 1), PIP and AQP-3 (Aquaporin-3).
In the first paragraph,
A cosmetic composition for skin soothing, skin moisturizing, alleviating itching and improving the skin barrier, characterized in that the cosmetic composition inhibits the expression of the Cox-2 (Cyclooxygenase-2) gene, which is an inflammatory factor.
In the first paragraph,
A cosmetic composition for skin soothing, skin moisturizing, itching relief and skin barrier improvement, characterized in that the cosmetic composition has skin irritation relief effects.
In paragraph 9,
A cosmetic composition for skin soothing, skin moisturizing, itching relief and skin barrier improvement, characterized in that the above skin irritation is erythema or itching.
In the first paragraph,
A cosmetic composition for skin soothing, skin moisturizing, alleviating itching and improving the skin barrier, characterized in that the cosmetic composition has a formulation selected from the group consisting of liquid, gel, emulsion, cream, foam, toner, pack, softening water, milk, foundation, makeup base, essence, soap, liquid cleanser, bath agent, sunscreen cream and sun oil.