KR20100089409A - Cosmetic composition for acne improvement - Google Patents

Abstract

PURPOSE: A cosmetic composition for treating acne containing herb plant extract is provided to ensure effective acne treatment without side effects. CONSTITUTION: An external use composition for acne contains mixture extract of Scutellariae radix and sprouting bean extracts, glycyrrhiza extract, and camomile roman flower extracts. The external use composition is a cosmetic composition. The cosmetic composition is used in the form of emulsion, lotion, cream(oil-in-water drop, water-in-oil drop, multiple phase), solution, suspension(anhydrous and hydrous), anhydrous product(oil and glycols), gel, mask, pack, or powder.

Description

Cosmetic composition for acne improvement

The present invention relates to a cosmetic composition for improving acne, and more particularly to a cosmetic composition for improving acne containing germinated soybean extract, magnolia bark extract, licorice extract, chamomile flower extract and golden extract.

Acne is an inflammatory disease that occurs in the hair follicle sebaceous glands of the skin, and is a disease that occurs mainly in young people.

The cause of acne is not clearly identified to date, but in general, it is known that it is caused by a combination of various causes.

There are four main causes of acne outbreaks: 1) growth of acne bacteria, Propionibacterium acnes , 2) sebum oversecretion associated with 5a-reductase activity, and 3) abnormal increase Old follicle wall hyperkeratosis, and 4) other inflammatory factors.

Therefore, the development of technology for preventing or treating acne has been focused on eliminating the above causes.

For example, U.S. Patent No. 5,380,763, Korean Laid-Open Patent Publication No. 10-2003-0017295, and Korean Patent No. 441420, etc., are proliferative inhibitors of acne bacteria propionibacterium acnes (1-pentadecane in the case of U.S. Patent No. 5,380,763). All (1-pentadecanol) is the inhibitor, and in the case of Korean Patent Publication No. 10-2003-0017295, grapefruit seed, red ginseng or Neem fruit extract is the inhibitor, and Korean Patent No. 441420 discloses Lactococcus spis HY Lactobiocin produced by 49 is an inhibitor, and US Pat. No. 6,174,892 discloses finasteride, an inhibitor of 5a-reductase activity, an enzyme associated with sebum hypersecretion. And preventing or treating acne using the same. However, these substances inhibit one mechanism of action, so the practical effect is somewhat insignificant.

Therefore, the present invention uses germination bean extract, magnolia bark extract, licorice extract, chamomile flower extract, and golden extract to inhibit the growth of acne bacteria Propionibacterium acnes and the enzyme 5a-reductase inhibitory activity related to sebum oversecretion. Disclosed is a cosmetic composition for improving acne having a back and the like.

It is an object of the present invention to provide a cosmetic composition for improving acne better than conventionally developed products without skin irritation using natural extracts.

The present invention seeks to provide a cosmetic composition with excellent acne-improving effect by selecting natural extracts having a proliferative inhibitory activity of acne bacteria propionibacterium acnes and 5a-reductase inhibitory activity, an enzyme related to sebum oversecretion.

Acne improvement cosmetic composition containing germinated soybean extract, magnolia bark extract, licorice extract, chamomile flower extract, golden extract is very excellent in acne improvement without side effects such as skin irritation.

The present invention provides a cosmetic composition for improving acne comprising a germinated soybean extract, magnolia bark extract, licorice extract, chamomile extract and golden extract as an active ingredient.

In the following Examples and Experimental Examples it was confirmed that the mixed samples of germinated soybean extract or germinated soybean extract and licorice extract, chamomile flower extract has an inhibitory effect of inflammatory cytokine production and 5a-reductase inhibitory activity involved in sebum oversecretion, It is also confirmed that the magnolia bark extract or the mixture of magnolia bark extract and the golden extract has excellent antimicrobial activity against the propioni bacterium acnes, an acne bacterium.

Also in actual clinical trials conducted with a lotion containing germinated soybean extract, magnolia bark extract, licorice extract, chamomile flower extract and golden extract, germinated soybean extract, magnolia bark extract, licorice extract, chamomile flower extract and golden extract are excellent acne. It is clearly confirmed that it has an improvement effect.

The external preparation composition for acne improvement of this invention is provided based on these experimental results.

The following terms herein have the meanings as defined below.

First, "germinated bean" is defined as a plant that is artificially or spontaneously suppressed the development of bony leaves, in other words, is defined as a plant before the development of bony leaves. Therefore, artificially or naturally-differentiated from the seeds to the cotyledon, the plant and the embryonic axis (bean-eye) is not artificially or spontaneously yet differentiated into the cotyledon, and includes plants composed of the hypocotyl and the seed. In addition, since the differentiation of the cotyledon is artificially suppressed, normal bean sprouts, especially young bean sprouts having a short growing period, are considered to be included in the category of germinated soybeans. It is understood here that there are no particular restrictions on the type of beans that can be used for the cultivation of germinated beans. In general, beans are divided into shapes, colors, and uses, and include peas, kidney beans (including hindu beans and red kidney beans), seomoktae, cheontae, eastern, chestnut, white tae (mekong), herb beans, rice beans, bean beans, frost tea, and green beans. Glycine subgenus ( G. albicans) Tind and Craven; G. arenaria Tind ; G. argyrea Tind ; G. canescens FJ Herm ; G. clandestina Wendl ; G. curvata Tind ; G. cyrtoloba Tind ; G. falcata Benth ; G. hirticaulis Tind . and Craven; G. lactovirens Tine. and Craven; G. latifolia (Benth.) Newell & Hymowitz ; G. latrobeana ( Meissn .) Benth ; G. microphylla (Benth.) Tind ; G. pindanica Tind . and Craven; G. tabacina ( Labill ) Benth ) and Soja subgenus ( G. soja Sieb . & Zucc ; G. max (L.) Merr . Forma gracilis Skvortz ), all of these beans can be used for the cultivation of germinated beans. .

The next "magnolia bark" refers to the epidermis forming the woody outer layer of the magnolia trunk. Magnolia here means the buttercup magnolia and all plants belonging to the genus Magnolia. Therefore, the magnolia includes Magnolia kobus, Magnolia heptapeta, Magnolia quinquepeta, Magnolia sieboldii, Magnolia grandiflora, Magnolia hypoleuca, Magnolia stellata, etc. It is to be understood that all are included.

Next, "a germinated soybean extract", "magnolia bark extract", "licorice extract", "chamomile flower extract" and "golden extract" is a solution obtained by crushing germinated soybeans, magnolia bark, licorice, chamomile flowers and gold and removing solids It is defined as containing extracts obtained by extracting the extracts of the state and germinated soybeans, magnolia bark, licorice, chamomile flowers and gold in polar and nonpolar extract solvents. Extractive solvents that can be used here include, for example, water, distilled water, alcohols (preferably lower alcohols having 5 or less carbon atoms), ethyl acetate, acetone, chloroform, methylene chloride or mixed solvents thereof. Although not disclosed in the following Examples and Experimental Examples, the inventors of the present invention also show that the germinated soybean extract, magnolia bark used in the following examples are also obtained by immersing germinated soybeans, magnolia bark, licorice, chamomile flowers and gold in ethanol. Similar to the extract, licorice extract, chamomile flower extract and golden extract has been confirmed to have a high acne improvement effect.

The following "acne" is defined to include any disease classified as acne in the art, regardless of the direct or indirect cause of its occurrence. As can be seen in the following experimental example of the present invention using a cosmetic composition containing germinated soybean extract, magnolia bark extract, licorice extract, chamomile flower extract and golden extract in 40 subjects with randomly selected acne disease Experiments have shown that it is effective for up to about 90% of the subjects. These results are obtained from a randomly selected subjects, the external composition for acne improvement containing germinated soybean extract, magnolia bark extract, licorice extract, chamomile flower extract and golden extract of the present invention is the occurrence of acne Regardless of the cause, it can be seen as effective for all types of acne. Therefore, the meaning of the acne is, for example, collective acne, acne caused by bromide, general acne, congoblate acne, acne by using cosmetics, acne by using detergent, acne dtergicans, adolescent acne , Acne fulminans, acne furunculoid, halogenated acne, callus acne, keloid acne, physical acne, mechanical acne, drug acne, necrotic acne Acne, neonatal acne (nene neonatorum), oily acne, papule acne, pomade acne, premenstrual acne, acne rosacea, swelling acne, tropical acne, addictive Acne (acne venenata), acne vulgaris and the like are all included.

The following "improvement" means treatment, prevention and alleviation of symptoms.

On the other hand, in the external preparation composition for improving acne of the present invention, the active ingredient germinated soybean extract, magnolia bark extract, licorice extract, chamomile flower extract and golden extract have a toxicity to the human body depending on the degree of acne symptoms of the skin It may be included in any amount within the range, specifically, may be included in 0.001% by weight to 99.99% by weight based on the total weight of the composition, preferably may be included in 0.001% to 20% by weight.

The external preparation composition for improving acne of the present invention is a compound known to have an acne improving effect so as to enhance or enhance the acne improving effect, in addition to the germinating bean extract, magnolia bark extract, licorice extract, chamomile flower extract, and golden extract, which are the active ingredients. Or plant extracts, such as vitamin A, phytandiolamine (WO 2003/037845), benzofuran derivatives (US Pat. No. 4,775,663), isoflavones (Korean Patent No. 439147), tetracycline compounds, benzoyl Peroxide, triclosan, salicylic acid, retinyl palmitate, octoxyglycerol, rosemary extract, sage extract, antihistamine (Hydroxyzine, Acrivastine, etc.) and the like, but are not limited thereto.

In another aspect, the present invention can be identified as an external preparation composition for improving skin troubles including germinated soybean extract, magnolia bark extract, licorice extract, chamomile flower extract and golden extract as an active ingredient.

In the present specification, "skin trouble" is defined as meaning including acne, skin inflammation, itching, and redness of the skin when referring to the following experimental example.

In the external preparation composition for improving skin troubles of the present invention, the amount of the active ingredient, such as germinated bean extract, magnolia bark extract, licorice extract, chamomile flower extract and golden extract, should be included in the acne improvement of the present invention. The bar described with respect to the external preparation composition is effective as it is.

The above-described composition of the present invention, that is, the external preparation composition for improving acne and the external preparation for improving skin trouble (hereinafter, the "composition of the present invention") can be understood as a cosmetic composition in a specific embodiment.

When the composition of the present invention is conceived as a cosmetic composition, the cosmetic composition can be prepared in various forms, for example, emulsions, lotions, creams (oil-in-water, water-in-oil, multiphase), solutions, suspensions (anhydrous and Aqueous), anhydrous products (oil and glycol based), gels, masks, packs, powders and the like.

The cosmetic composition of the present invention may include an acceptable carrier in cosmetic preparations in addition to germinated bean extract, magnolia bark extract, licorice extract, chamomile flower extract and golden extract.

Herein, "acceptable carriers in cosmetic preparations" are compounds or compositions already known and used that can be included in cosmetic preparations, or compounds or compositions that will be developed in the future, which are toxic, unstable or irritating beyond contact with the human body upon contact with the skin. Say nothing.

The carrier may be included in the cosmetic composition of the present invention in an amount from about 1% to about 99.99% by weight, preferably from about 90% to about 99.99% by weight of the composition.

However, since the ratio will vary depending on the formulation as described above in which the cosmetic composition of the present invention is prepared and its specific application site (face, neck, etc.) or its preferred application amount, the ratio is in any aspect the present invention. It should not be understood as limiting the scope of.

Meanwhile, examples of the carrier include alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizers, viscosity modifiers, emulsions, stabilizers, sunscreen agents, colorants, fragrances, and the like.

The alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, moisturizers, viscosity modifiers, emulsions, stabilizers, sunscreens, colorants, compounds / compositions that can be used as fragrances and the like are already known in the art, Appropriate materials / compositions may be selected and used.

Meanwhile, the composition of the present invention may be regarded as a pharmaceutical composition in another specific embodiment.

When the composition of the present invention is identified as a pharmaceutical composition, the pharmacological effect may be identified as an acne improvement effect and skin trouble improvement effect as described above.

The pharmaceutical composition of the present invention may include a pharmaceutically acceptable carrier in addition to the germinated bean extract, magnolia bark extract, licorice extract, chamomile flower extract and golden extract as an active ingredient, such a pharmaceutically acceptable carrier may be a pharmaceutical formulation. As commonly used in the city, lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia rubber, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, cellulose, water , Syrups, methyl cellulose, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oils, and the like. The pharmaceutical compositions of the present invention may also further comprise lubricants, wetting agents, sweetening agents, flavoring agents, emulsifying agents, suspending agents, preservatives and the like as additives.

The carrier may be included in the pharmaceutical composition of the present invention in an amount of about 1% to about 99.99% by weight, preferably about 90% to about 99.99% by weight, based on the total weight thereof, and the additive is about 0.1% by weight. To about 20% by weight.

On the other hand, the pharmaceutical composition of the present invention is administered directly to the skin in a topical manner.

The pharmaceutical compositions of the present invention can be prepared in unit dose form or formulated into pharmaceutically acceptable carriers and / or excipients or incorporated into multi-dose containers. In this case, the formulation may be in the form of a solution, a suspension or an emulsion, or may include an exercire, an excipient, a powder, a granule, a tablet, a warning, a coughing agent, a lotion, an ointment, and the like.

The daily dosage of the pharmaceutical composition of the present invention is usually 0.001 ~ 150 mg / kg body weight range, it can be administered once or divided into several times. However, since the dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as the route of administration, the age, sex, weight of the patient, the severity of the patient and the like, the dosage may limit the scope of the present invention in any aspect. It should not be understood as.

The composition of the present invention may be regarded as a soap composition in another specific embodiment.

When the composition of the present invention is identified as a soap composition, the soap composition of the present invention may be prepared by including a germinated soybean extract, magnolia bark extract, licorice extract, chamomile flower extract and golden extract on the soap base, and as an additive to the skin It may be prepared including a moisturizer, emulsifier, water softener and the like.

As the soap base material, vegetable oils such as palm oil, palm oil, soybean oil, perm oil, olive oil, palm kernels, or animal oils such as tallow, pork, sunny and fish oils can be used, and the skin moisturizing agent is glycerin, erythritol and polyethylene glycol. , Propylene glycol, butylene glycol, pentylene glycol, hexyl glycol, isopropyl myristate, silicone derivatives, aloe vera, sorbitol and the like can be used, the emulsifiers include natural oils, wax fatty alcohols, hydrocarbons, natural plant extracts, etc. May be used, and tetrasodium idieti or the like may be used as the water softener.

The soap composition of the present invention may further include an antimicrobial agent, a dispersant, a foam inhibitor, a solvent, a scale inhibitor, a corrosion inhibitor, a perfume, a pigment, a metal ion sequestrant, an antioxidant, a preservative, and the like as an additive.

In the soap composition of the present invention, the soap base or additives may be included in an amount generally used in the art, and the soap base is generally added in an amount of 99.999 wt% to 50 wt% based on the total weight of the soap composition. The additive may be added in an amount of 1% to 20% by weight.

In another aspect, the present invention provides a composition for inhibiting cytokine production, including germinated bean extract, licorice extract and chamomile flower extract.

Cytokines are factors that modulate the interactions between cells involved in immune, inflammation, or hematopoietic responses, including interleukin-1, interleukin-6, interleukin-8, tumor necrosis factor (TNF-a), and the like. Excessive or unregulated cytokine production and secretion disrupts immunomodulatory functions to mediate or exacerbate various diseases, such as allergic diseases, inflammatory diseases, autoimmune diseases, and the like.

Therefore, the composition for inhibiting cytokine production of the present invention may be useful for improving the disease, and may be particularly useful for improving acne.

In the composition for inhibiting cytokine production of the present invention, the cytokine is preferably interleukin-8 or tumor necrosis, whose production inhibitory effect is directly confirmed by the germinated bean extract, licorice extract and chamomile flower extract in the following Examples and Experimental Examples. It can be understood as a factor (TNF-a).

When the composition for inhibiting cytokine production of the present invention is regarded as a cosmetic composition or a pharmaceutical composition in a specific embodiment, the above-described effective contents thereof are effective as it is for its effective amount, administration method and formulation.

In another aspect, the present invention provides a topical composition for improving acne comprising a germinated soybean extract and / or extracts of plant organs derived from magnolia.

In the above, "plant organs derived from magnolia" are considered to be classified into reproductive organs such as flowers, fruits and seeds, and nutritional organs such as roots, stems and leaves. , Seed of magnolia, root of magnolia, and trunk of magnolia. Although the Examples and Experimental Examples of the present invention looked at the acne improvement effect with the magnolia bark extract, those skilled in the art will check and select whether there is an acne improvement effect against other magnolia-derived plant organ extracts within the range of their usual ability. Because you can do it.

Therefore, for example, if the extract of the magnolia seed has an acne improving effect, the external preparation composition for acne improvement comprising the extract of the magnolia seed as an active ingredient is also considered to be included in the scope of the present invention.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, it should not be understood that the scope of the present invention is limited to these examples and experimental examples.

< Example > Manufacture of Natural Extracts

Example 1 Germinated Bean Extract Preparation

The sprouts were 1-10 cm in length, and a proper amount of water was added to the sprouts, and then uniformly crushed using a mixer, filtered and concentrated with filter paper. The crushed concentrate was centrifuged again to discard the submerged solids, and only the supernatant was recovered, followed by filtering with a 0.45 micrometer filter to prepare a final extract.

Example 2 Preparation of Magnolia Bark Extract

An appropriate amount of water was added to the bark of the magnolia tree, which was then crushed uniformly using a mixer, filtered through a filter paper, and concentrated. The remaining extraction process was the same as in <Example 1>.

Example 3 Licorice Extract Preparation

Licorice and an appropriate amount of water were mixed and crushed uniformly using a mixer, and then extracted with hot water at a temperature of 80 to 120 for 1 to 3 hours, filtered through a filter paper, and concentrated. The remaining extraction process was the same as in <Example 1>.

Example 4 Chamomile Flower Extract Preparation

The same method as in <Example 3> was used.

Example 5 Preparation of Golden Extract

The same method as in <Example 3> was used.

Example 6 Preparation of Mixture of Germinated Bean Extract, Licorice Extract, and Chamomile Flower Extract

The germinated soybean extract, licorice extract and chamomile flower extract prepared by the method of <Example 1>, <Example 3>, and <Example 4> were used in the same ratio.

Example 7 Preparation of Mixture of Magnolia Bark Extract and Golden Extract

The magnolia bark extract and the golden extract prepared in <Example 2> and <Example 5> were used in the same ratio.

< Production Example > Preparation of Cosmetic Composition

To prepare a cosmetic composition comprising the extract of <Example 1> to <Example 5>. In the following Table 1, raw materials 1 to 11 were mixed and dissolved at 70 to obtain water parts, and raw materials 12 to 19 were dissolved at 70 to obtain oil parts. The oil part was added to the water part, stirred with a homomixer (Tokushu Kika, Japan) and emulsified first, and the raw material 16 was added to increase. After removing the bubbles, it was cooled to room temperature to prepare a cosmetic composition of the nutrient cosmetic formulation.

Raw material of cosmetic composition ingredient Preparation Example 1 Production Example 2 Production Example 3 Comparative example 1. Purified water To 100 To 100 To 100 To 100 2. Glycerin 8.0 8.0 8.0 8.0 3. Butylene Glycol 4.0 4.0 4.0 4.0 4. Hyaluronic Acid Extract 5.0 5.0 5.0 5.0 5. Beta Glucan 7.0 7.0 7.0 7.0 6. Carbomer 0.1 0.1 0.1 0.1 7. Germinated Bean Extract 3 - 3 - 8. Magnolia Bark Extract - 3 3 - 9. Licorice Extract 3 - 3 - 10. Chamomile Flower Extract 3 - 3 - 11.Gold Extract - 3 3 - 12. Caprylic Capric Trigle
Riseide 8.0 8.0 8.0 8.0 13. Squalane 5.0 5.0 5.0 5.0 14. Cetearyl Glucoside 1.5 1.5 1.5 1.5 15. Sorbitan Stearate 0.4 0.4 0.4 0.4 16. Cetearyl Alcohol 1.0 1.0 1.0 1.0 17. Preservative Quantity Quantity Quantity Quantity 18. Incense Quantity Quantity Quantity Quantity 19. Triethanolamine 0.1 0.1 0.1 0.1

On the other hand, in order to use as a cosmetic composition of <Comparative Example>, as shown in the <Table 1> was prepared a cosmetic composition does not contain germinated soybean extract, magnolia bark extract, licorice extract, chamomile flower extract, golden extract.

< Experimental Example > Measures for improvement on acne

Experimental Example 1 Inhibitory Effects of Extracts on Inflammatory Cytokine Production

The monocytic THP-1 cell line derived from humans was used to treat extracts of <Example 1> or <Example 6> at the concentrations of <Table 2> and <Table 3>, followed by Propionibacterium acnes ( P. acnes ) strain. Inhibitory effects of the induced inflammatory cytokines, interleukin-8 (IL-8) and tumor necrosis factor-alpha (TNF-a), were measured. Human-derived monocytic THP-1 cell lines were cultured at 37 and 5% CO ₂ with Roswell Park Memorial Institute 1640 medium (RPMI 1640) added with 10% fetal bovine serum. After culturing the cells at a concentration of 10 ⁶ cells / well in a 24-well plate and confirming the cell attachment, P. acnes strain was added to the cell culture to induce the production of inflammatory cytokines, and then The test materials were added simultaneously and incubated for 2 days. Here, the test substance is the extract of <Example 1> or <Example 6> is diluted to the test concentration with the cell culture solution and mixed in the same ratio. Inflammatory cytokines present in the culture were measured using an Enzyme-Linked Immunosorbent Assay (ELISA) Kit capable of analyzing each cytokine. Inhibition rate of inflammatory cytokines was calculated according to the following formula and the results are shown in Tables 2 and 3.

Inhibitory rate of inflammatory cytokine production (%) = 100- (production amount in the presence of each test substance / production amount of P. acnes treated control group X 100)

Inhibitory Effect of Interleukin-8 Production Test substance (treatment concentration) % Inhibition Germinated Bean Extract 0.01% 6.0 Germinated Bean Extract 0.1% 16.2 Germinated Bean Extract 1.0% 29.5 (Germinated Beans + Licorice + Chamomile Flower) Extract 0.01% 7.2 (Grow Bean + Licorice + Chamomile Flower) Extract 0.1% 20.0 (Grow Bean + Licorice + Chamomile Flower) Extract 1.0% 38.9 P. acnes (control) 0

Inhibitory Effect of Tumor Necrosis Factor-alpha Production Test substance (treatment concentration) % Inhibition Germinated Bean Extract 0.01% 5.2 Germinated Bean Extract 0.1% 8.7 Germinated Bean Extract 1.0% 35.6 (Germinated Beans + Licorice + Chamomile Flower) Extract 0.01% 6.2 (Grow Bean + Licorice + Chamomile Flower) Extract 0.1% 11.0 (Grow Bean + Licorice + Chamomile Flower) Extract 1.0% 44.5 P. acnes (control) 0

From Table 2 and Table 3, it can be seen that the mixed test substance of the germinated bean extract of <Example 1> and <Example 6> shows the inhibitory effect of inflammatory cytokine production in a concentration-dependent manner, It can be seen that the effect of the mixed test material using the germinated soybean extract, licorice extract and chamomile flower extract is much better than the extract alone.

Experimental Example 2 Inhibitory Effect of Extracts on 5a-reductase Enzyme Activity

5a-reductase is the most important enzyme in male hormone biosynthesis and is the most important factor in puberty acne production through excess sebum production. By measuring the inhibitory effect of the test substance on 5a-reductase, the indirect effects on sebum production and acne inhibition can be identified. The extracts of <Example 1> or <Example 6> were treated with the culture solution of HEK293 (Human Embryonic Kidney) cell line, which is a human-derived 5a-reductase-secreting cell, to secrete 5a-reductase to <Example 1> or <Example It was determined whether it was inhibited by the extract treatment of Example 6>. HEK293 cell line, a 5a-reductase-secreting cell, was cultured at 37 and 5% CO ₂ in medium containing 10% fetal bovine serum to RPMI 1640. After culturing the cells at a concentration of 10 ⁶ cells / well in a 24-well plate and confirming cell adhesion, test substances (<Example 1> or <Example 6>) were added to the cell culture solution by concentration. Incubate for 24 hours. The culture medium was recovered, and according to the method of Baerbel P. et al. (Mol Cell Biochem 270: 201-208, 2005), the substrate 4-androstene-3,17-dione was reacted with the action of 5a-reductase 5a-androstane-3,17 The rate of change to -dione was measured. 5a-reductase enzyme activity inhibition rate was calculated according to the following formula, and the results are shown in <Table 4>.

Inhibition rate of enzyme activity (%) = 100- (production amount in the presence of each test substance / substrate change amount of control group X 100)

Inhibition rate of 5a-reductase Test substance (treatment concentration) Enzyme Inhibition Rate (%) Germinated Bean Extract 1.0% 13.1 Germinated Bean Extract 3.0% 41.0 (Grow Bean + Licorice + Chamomile Flower) Extract 1.0% 17.5 (Germinated Beans + Licorice + Chamomile Flower) Extract 3.0% 52.4 Control 0

As can be seen in Table 4, the germinated bean extract of <Example 1> and the mixed test substance of <Example 6> inhibit 5a-reductase activity, an enzyme important for sebum production in a concentration-dependent manner. In addition, it can be seen that the effect of the mixed test material using the germinated bean extract, licorice extract, chamomile flower extract than when using the germinated bean extract alone.

Experimental Example 3 Determination of Antimicrobial Effects of Extracts against Acne Bacteria

(1) antibacterial effect against acne

Determination of the antimicrobial effect against acne bacteria ( Propionibacterium acnes ATCC6919) was carried out according to the Kirby-Bauer standard method. Acne bacteria 10 ⁶ cultured in anaerobic conditions in a liquid heart (brain heart infusion broth) are mixed with 7 milliliters (ml) of a plate medium (containing 0.8% phytoagar) and hardened by petri dishes. Then, a filter paper disc having a diameter of 10 millimeters (mm) moistened with the extract of Example 2 or Example 7 was placed on the surface of the medium, and cultured under anaerobic conditions for 37 to 24 hours. The antimicrobial effect was confirmed by measuring the diameter of the clear zone in which the growth of acne bacteria was suppressed, and the results are shown in Table 5.

Antimicrobial Effects on P. acnes Substance (treatment concentration) Clear Zone (mm) Magnolia Bark Extract 0.25% 9.3 Magnolia Bark Extract 0.5% 13.6 Magnolia Bark Extract 2.0% 22.5 (Magnolia Bark + Golden) Extract 0.25% 10.9 (Magnolia Bark + Golden) Extract 0.5% 15.0 (Magnolia Bark + Golden) Extract 2.0% 26.7 Control 0

As can be seen in Table 5, the extracts of <Example 2> and <Example 7> it can be confirmed that the antibacterial effect against acne bacteria. In addition, it was confirmed that the antibacterial effect of the test substance mixed with the magnolia bark extract and the golden extract was much better than the antibacterial effect of the test substance using the magnolia bark extract alone.

(2) Measurement of minimal inhibitory concentration (MIC)

Acne bacteria ( Propionibacterium acnes ATCC6919) was incubated in anaerobic conditions for 24 hours at 37 in 10 milliliters of brain heart infusion broth. The extract of Example 2 or Example 7 was added to the culture solution of the acne bacteria, and then cultured further for 24 hours. The minimum inhibitory concentration (MIC) was determined as the minimum concentration of the test substance without growth of test bacteria, and the results are shown in <Table 6>.

MIC for P. acnes Test bacteria MIC (%) Magnolia bark Magnolia Bark + Golden P. acnes 0.0125 0.009

Experimental Example 4 Safety Confirmation Experiment on Human Skin of Composition

Prior to experimenting with the acne improvement effect of the cosmetic compositions of <Preparation Examples 1, 2 and 3> and <Comparative Examples>, a cumulative stimulation test of human skin was conducted to verify the skin safety of the compositions. A total of nine 24-hour cumulative patches are given every other day on the upper forearm in 20 healthy adults. The patch method uses a Finn chamber or a similar kind of chamber, in which 15 compositions are added per chamber, followed by patching. Calculations and the results are shown in Table 7 below. At this time, ± 1 point in the reaction, + 2 points, ++ gives a score of 4 points (highest score), when the average response is less than 3 is determined as a safe composition.

Average responsiveness = (number of respondents x responsiveness / total number of subjects x highest score x 100) ÷ total number of tests

Results of skin reactions during cumulative patch tests Test substance Number of subjects with response Average
Responsiveness 1 week 2 weeks 3 weeks Primary Secondary 3rd 4th 5th 6th 7th 8th 9th
Preparation Example 1
Preparation Example 2
Preparation Example 3 ± + ++ ± + ++ ± + ++ ± + ++ + ++ ± + ++ ± + ++ ± + ++ ± + ++ - - - - - - - - - - - - - - - - - - - - - - - - - - - - 0.00 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 0.00 - - - - - - - - - - - - - - - - - - - - - - - - - - - - 0.00 Comparative example - - - - - - - - - - - - - - - - - - - - - - - - - - - 0.00 Inspector 20 20 20 20 20 20 20 20 20

From the results of Table 7, all the compositions of Preparation Example did not show cumulative stimulation pattern as in the composition of Comparative Example.

<Experiment 5> Test for improving acne on human skin

40 volunteers with acne symptoms on the face were divided into four groups, each of which provided the composition of <Production Example 1>, <Production Example 2> and <Production Example 3>, and the last group <Comparative Example> A composition of> was applied to the acne site for one week twice a morning and evening. Determination of the skin improvement effect was to write the degree of symptom relief of the participant in the questionnaire, the results are calculated according to the following formula and are shown in <Table 8>, the photo showing the degree of acne skin improvement is shown in Figure 1 .

Degree of improvement = enhanced subjects / total subjects x 100

Improved results for acne skin Test substance Itching improvement Skin redness improvement Improved skin inflammation Production Example 3 94.0 (%) 90.0 (%) 95.0 (%) Production Example 2 40.0 (%) 55.0 (%) 55.0 (%) Preparation Example 1 45.0 (%) 34.0 (%) 34.0 (%) Comparative example 20.0 (%) 10.0 (%) 10.0 (%)

Table 8 shows <Production Example 1> containing germinated soybean extract, licorice extract, and chamomile flower extract having excellent anti-inflammatory effect, and <Preparation Example 2> containing magnolia bark extract and golden extract having excellent antibacterial effect. In the case of the cosmetic composition containing it also shows an improvement effect on acne skin, but the composition of <Production Example 3> containing the germinated soybean extract, magnolia bark extract, licorice extract, chamomile flower extract, golden extract at the same time acne The improvement effect on the skin is very excellent.

< Formulation example > Of cosmetic composition Formulation example

The various compositions of the present invention have been described below for the preparation of cosmetic compositions and the preparation of pharmaceutical compositions in view of the fact that it will be mainly used as a cosmetic composition and a pharmaceutical composition.

<Example 1> Nutritional Longevity (Milk Skin Lotion) Formulation example

Here, the extract refers to a mixture of germinated soybean extract, magnolia bark extract, licorice extract, chamomile flower extract and golden extract revealed in the composition and operation of the invention.

Nutritional Ingredients and Ingredient Ratio 1. Purified water To 100 2. Glycerin 8.0 3. Butylene Glycol 4.0 4. Hyaluronic Acid Extract 5.0 5. Beta Glucan 7.0 6. Carbomer 0.1 7. Extract Quantity 8. Caprylic Capric Triglycerides 8.0 9. Squalane 5.0 10. Cetearyl Glucoside 1.5 11.Sorbitan Stearate 0.4 12. Cetearyl Alcohol 1.0 13. Preservative Quantity 14. Incense Quantity 15. Pigment Quantity 16. Triethanolamine 0.1

<Preparation Example 2> For preparation of nourishing cream

Here, the extract refers to a mixture of germinated soybean extract, magnolia bark extract, licorice extract, chamomile flower extract and golden extract revealed in the composition and operation of the invention.

Ingredients and ingredients ratio of nutrition cream 1. Purified water To 100 2. Glycerin 3.0 3. Butylene Glycol 3.0 4. Liquid paraffin 7.0 5. Beta Glucan 7.0 6. Carbomer 0.1 7. Extract Quantity 8. Caprylic Capric Triglycerides 3.0 9. Squalane 5.0 10. Cetearyl Glucoside 1.5 11.Sorbitan Stearate 0.4 12. Polysorbate 60 1.2 13. Preservative Quantity 14. Incense Quantity 15. Pigment Quantity 16. Triethanolamine 0.1

Preparation Example 3 Formulation Example of Massage Cream

Here, the extract refers to a mixture of germinated soybean extract, magnolia bark extract, licorice extract, chamomile flower extract and golden extract revealed in the composition and operation of the invention.

Ingredients and proportions of massage cream 1. Purified water To 100 2. Glycerin 8.0 3. Butylene Glycol 4.0 4. Liquid paraffin 45.0 5. Beta Glucan 7.0 6. Carbomer 0.1 7. Extract Quantity 8. Caprylic Capric Triglycerides 3.0 9. Beeswax 4.0 10. Cetearyl Glucoside 1.5 11.Sesqui oleic acid sorbitan 0.9 12. Vaseline 3.0 13. Preservative Quantity 14. Incense Quantity 15. Pigment Quantity 16. Paraffin 1.5

<Formulation Example 4> For preparation of the pack

Here, the extract refers to a mixture of germinated soybean extract, magnolia bark extract, licorice extract, chamomile flower extract and golden extract revealed in the composition and operation of the invention.

Ingredients and proportions of the pack 1. Purified water To 100 2. Glycerin 4.0 3. Polyvinyl Alcohol 15.0 4. Hyaluronic Acid Extract 5.0 5. Beta Glucan 7.0 6. Allantoin 0.1 7. Extract Quantity 8. Nonyl Phenyl Ether 0.4 9. Polysorbate 60 1.2 13. Preservative Quantity 14. Incense Quantity 15. Pigment Quantity 16. Ethanol 6.0

<Formulation Example 5> Formulation Example of Ointment

Here, the extract refers to a mixture of germinated soybean extract, magnolia bark extract, licorice extract, chamomile flower extract and golden extract revealed in the composition and operation of the invention.

Ointments and their proportions 1. Purified water To 100 2. Glycerin 8.0 3. Butylene Glycol 4.0 4. Liquid paraffin 15.0 5. Beta Glucan 7.0 6. Carbomer 0.1 7. Extract Quantity 8. Caprylic Capric Triglycerides 3.0 9. Squalane 1.0 10. Cetearyl Glucoside 1.5 11.Sorbitan Stearate 0.4 12. Cetearyl Alcohol 1.0 13. Preservative Quantity 14. Incense Quantity 15. Pigment Quantity 16. Beeswax 4.0

<Preparation Example 6> Preparation of oil products, for example,

Here, the extract refers to a mixture of germinated soybean extract, magnolia bark extract, licorice extract, chamomile flower extract and golden extract revealed in the composition and operation of the invention.

Composition and proportions of oil products Total amount 100.0 1. Squalane 5.0 2. Macadamia Nut Oil 5.0 3. Jojoba Oil 1.0 4. Crude colostrum 0.5 5. Wild Rose Seed Extract 20.0 6. Extract Quantity 7. Carlylic Capric Triglycerides 30.0 8. Liquid paraffin Balance 9. Incense Quantity

Acne improvement effect on human skin

Claims (4)

An external preparation composition for improving acne containing an extract consisting of germinated bean extract, licorice extract, chamomile flower extract, magnolia bark extract, and golden extract. An external preparation composition for improving skin troubles comprising an extract consisting of germinated bean extract, licorice extract, chamomile flower extract, magnolia bark extract and golden extract. The method according to claim 1 or 2, The external composition is a composition, characterized in that the cosmetic composition. The method according to claim 1 or 2, The external composition is a composition, characterized in that the pharmaceutical composition.

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