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KR927003804A - 가교제와 1-아미노에틸렌기를 함유하는 중합체로 가교결합에 의한 효소의 고정화 방법 및 제제 - Google Patents

가교제와 1-아미노에틸렌기를 함유하는 중합체로 가교결합에 의한 효소의 고정화 방법 및 제제 Download PDF

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KR927003804A
KR927003804A KR1019920701117A KR920701117A KR927003804A KR 927003804 A KR927003804 A KR 927003804A KR 1019920701117 A KR1019920701117 A KR 1019920701117A KR 920701117 A KR920701117 A KR 920701117A KR 927003804 A KR927003804 A KR 927003804A
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immobilized enzyme
enzyme preparation
crosslinking agent
biological material
polymer
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KR1019920701117A
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페더젠 스벤
빌 제르겐센 올레
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안네 제케르
노보 노르디스크 아크티에 셀스카브
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • C12N11/091—Phenol resins; Amino resins

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  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Processes Of Treating Macromolecular Substances (AREA)

Abstract

내용 없음

Description

가교제와 1-아미노에틸렌기를 함유하는 중합체로 가교결합에 의한 효소의 고정화 방법 및 제제
[도면의 간단한 설명]
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (13)

  1. a) 효소학적활성의 생물학적물질을 함유하는 수성매제를 제공하는 단계, b) 1-아미노에틸렌기 및 임의적으로 N-비닐 포름아미드를 힘유하는 중합체를 첨가하는 단계, c) 아미노기에 대한 가교제를 첨가하는 단계, d) 가교결합 및 솜털모양화를 실행하기 위해 혼합물을 유지시키는 단계, e) 탈수단계, f) 세분단계, 및 g) 건조단계의 연속단계들로 이루어지는 방법에 의해 얻을 수 있는 입상고정화 효소제제.
  2. 제1항에 있어서, 상기 중합체의 양은 생물학적물질의 건조물질의 2-30중량%, 바람직하게는 2-15%인 것을 특징으로 하는 고정화 효소제제.
  3. 제1항 또는 제2항에 있어서, 상기 중합체는 10-100몰%(바람직하게는 25-50%)의 -CH2-CH(NH2)-단위와 0-90몰%(바람직하게는 50-75% 의 -CH2-CH(NH-CHO)-단위를 함유하며, 바람직하게는 50,000-500,000의 분자량을 갖는 것을 특징으로 하는 고정화 효소제제.
  4. 제1항 내지 제3항중 어느 하나에 있어서, 키토산이 단계 c)에서 가교제의 도입에 앞서, 바람직하게는 생물학적물질의 건조물질의 1-15중량%의 양으로 첨가되는 것을 특징으로 하는 고정화 효소제제.
  5. 제1항 내지 제4항중 어느 하나에 있어서, 상기 가교제는 글루타르알데히드, 폴리아제티딘 모는 디이소시아네이트인 것을 특징으로 하는 고정화 효소제제.
  6. 제1항 내지 제5항중 어느 하나에 있어서, 단계 a)의 수성매제에 가교제를 추가로 첨가하고, 단계 b)에서 중합체의 도입에 앞서 부분적 가교 결합을 실행하기 위해 유지시킴으로써 얻어질 수 있는, 단계 a) 및 단계 c)에 첨가된 가교제의 비가 바람직하게는 1:2 내지 1:4의 범위가 되는 것을 특징으로 하는 고정화 효소제제.
  7. 제1항 내지 제6항중 어느 하나에 있어서, 사용된 가교제의 총량이 생물학적 물질의 건조물질의 5-40중량%인 것을 특징으로 하는 고정화 효소제제.
  8. 제1항 내지 제7항중 어느 하나에 있어서, 상기 생물학적물질은 바람직하게 식물 또는 미생물 유래의 용해된 효소, 완전 세포들 또는 충분히 또는 부분적으로 분쇄된 세포들로부터의 세포덩어리로 이루어지는 것을 특징으로 하는 고정화 효소제제.
  9. 제1항 내지 제8항중 어느 하나에 있어서, 상기 생물학적물질은 효소학적 불활성의 단백질로 더 이루어지는 것을 특징으로 하는 고정화 효소제제.
  10. 제1항 내지 제9항중 어느 하나에 있어서, 생물학적물질은 글루코스이소머라제(바람직하게는 스트렙토미세스, 바실루스 또는 악티노 플라네스의 균주로부터 유도된것), 아미노펩티다제(바람직하게는 유도모나스로부터 유도된것), 페니실린아실라제(바람직하게는 푸자리움으로부터 유도된것), 니트릴라제(바람직하게는 로도코쿠스, 유도모나스 또는 브레비 박테리움으로부터의 것), 프록토실 트랜스퍼라제(바람직하게는 아스퍼질러스로부터의 것), 인베르타제(바람직하게는 사카로 미세스로부터의 것), 락타제(바람직하게는 글루이베로 미세스로부터의 것) 또는 시아니다제(바람직하게는 알칼리젠스로부터의 것)로 이루어지는 것을 특징으로 하는 고정화 효소제제.
  11. 효소촉매된 공정에의 제1항 내지 제10항중 어느 하나에 따르는 고정화 효소제제의 용도.
  12. 고정베드에서 고정화효소를 가진 연속공정에의 제1항 내지 제11항중 어느 하나에 따르는 고정화 효소제제의 용도.
  13. a) 효소학적활성의 생물학적물질을 함유하는 수성매제를 제공하는 단계, b) 1-아미노에틸렌기 및 임의적으로 N-비닐 포름아미드를 함유하는 중합체를 첨가하는 단계, c) 아이노기에 대한 가교제를 첨가하는 단계, d) 가교결합 및 솜털모양화를 실행하기 위해 혼합물을 유지시키는 단계, e) 탈수단계, f) 세분단계, 및 g) 건조단계의 연속단계들로 이루어지는 것을 특징으로 하는 효소학적 활성의 생물학적 물질을 고정화시키는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019920701117A 1989-11-23 1990-11-23 가교제와 1-아미노에틸렌기를 함유하는 중합체로 가교결합에 의한 효소의 고정화 방법 및 제제 Ceased KR927003804A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK5893/89 1989-11-23
DK589389A DK589389D0 (da) 1989-11-23 1989-11-23 Immobiliseret enzympraeparat
PCT/DK1990/000305 WO1991008287A1 (en) 1989-11-23 1990-11-23 Immobilization of enzymes by cross-linking with a cross-linking agent and a polymer containing 1-amino ethylene moieties

Publications (1)

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KR927003804A true KR927003804A (ko) 1992-12-18

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KR1019920701117A Ceased KR927003804A (ko) 1989-11-23 1990-11-23 가교제와 1-아미노에틸렌기를 함유하는 중합체로 가교결합에 의한 효소의 고정화 방법 및 제제

Country Status (10)

Country Link
US (1) US5279948A (ko)
EP (1) EP0502035B1 (ko)
JP (1) JPH05501499A (ko)
KR (1) KR927003804A (ko)
AT (1) ATE124720T1 (ko)
CA (1) CA2066686A1 (ko)
DE (1) DE69020741T2 (ko)
DK (1) DK589389D0 (ko)
FI (1) FI922360L (ko)
WO (1) WO1991008287A1 (ko)

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US5736624A (en) * 1994-12-02 1998-04-07 Abbott Laboratories Phosphatase activated crosslinking, conjugating and reducing agents; methods of using such agents; and reagents comprising phosphatase activated crosslinking and conjugating agents
AU6659296A (en) * 1995-07-18 1997-02-18 Gist-Brocades B.V. An improved immobilized penicillin g acylase
US6060268A (en) * 1995-07-18 2000-05-09 Gist-Brocades B.V. Penicillin G acylase immobilized with a crosslinked mixture of gelled gelatin and amino polymer
AU4560997A (en) * 1996-10-04 1998-05-05 Rhone-Poulenc Chimie Preparation of a composition containing a heat sensitive substance and a compound cross-linkable by heat and use of said composition
FR2755143B1 (fr) * 1996-10-25 1998-11-27 Rhone Poulenc Nutrition Animal Procede de preparation de l'acide 2-hydroxy-4-methylthio-butyrique par utilisation d'une nitrilase
US7521216B2 (en) 1999-12-29 2009-04-21 Verenium Corporation Nitrilases and methods for making and using them
US7608445B1 (en) 1999-12-29 2009-10-27 Verenium Corporation Nitrilases, nucleic acids encoding them and methods for making and using them
US7300775B2 (en) 1999-12-29 2007-11-27 Verenium Corporation Methods for producing α-substituted carboxylic acids using nitrilases and strecker reagents
WO2003106415A2 (en) 2002-06-13 2003-12-24 Diversa Corporation Processes for making (r)-ethyl 4-cyano-3-hydroxybutyric acid
WO2006109311A2 (en) * 2005-04-15 2006-10-19 Ramot At Tel Aviv University Ltd. Enzyme-channeling based electrochemical biosensors
JP2007101520A (ja) * 2005-09-09 2007-04-19 Mitsubishi Chemicals Corp 生体物質複合体、並びに、生体物質複合体担持体、対象物質の精製方法、アフィニティークロマトグラフィー用容器、分離用チップ、対象物質の解析方法、対象物質の解析用分離装置及びセンサーチップ
JP5209478B2 (ja) 2005-09-30 2013-06-12 ノボザイムス アクティーゼルスカブ 酵素の固定化
CN1982445B (zh) * 2005-12-16 2011-07-27 百瑞全球有限公司 用于制备固定化酶或固定化细胞的载体以及使用该载体的固定化方法
US7964415B2 (en) 2006-04-26 2011-06-21 Cardiogenics Inc. Stable water-soluble polyethylenimine conjugates and methods of use thereof
US9217164B2 (en) 2007-03-01 2015-12-22 Basf Enzymes Llc Nitrilases, nucleic acids encoding them and methods for making and using them
DE102007027195A1 (de) 2007-06-13 2008-12-18 Süd-Chemie AG Thermisch modifizierte Tonmineralien als Trägermaterialien für Enzyme
DE102007027206A1 (de) 2007-06-13 2008-12-18 Süd-Chemie AG Immobilisierung von Enzymen auf Bleicherden
US8889373B2 (en) 2010-08-12 2014-11-18 Eastman Chemical Company Enzyme catalyst immobilized on porous fluoropolymer support
CN104114700A (zh) * 2011-11-11 2014-10-22 吉尔德联合有限公司 一种可生物降解的固定化酶及其制备方法

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DK146942C (da) * 1978-04-19 1984-07-30 Novo Industri As Fremgangsmaade til fremstilling af et immobiliseret enzymprodukt
DE3128478A1 (de) * 1981-07-18 1983-02-03 Basf Ag, 6700 Ludwigshafen Verfahren zur herstellung von linearen, basischen polymerisaten
DK336987D0 (da) * 1987-07-01 1987-07-01 Novo Industri As Immobiliseringsmetode

Also Published As

Publication number Publication date
DK589389D0 (da) 1989-11-23
WO1991008287A1 (en) 1991-06-13
ATE124720T1 (de) 1995-07-15
DE69020741D1 (de) 1995-08-10
FI922360A7 (fi) 1992-05-22
DE69020741T2 (de) 1996-03-21
JPH05501499A (ja) 1993-03-25
EP0502035A1 (en) 1992-09-09
US5279948A (en) 1994-01-18
CA2066686A1 (en) 1991-05-24
EP0502035B1 (en) 1995-07-05
FI922360A0 (fi) 1992-05-22
FI922360L (fi) 1992-05-22

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