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KR980700433A - 디엔에이(dna) 시퀀싱 및 디엔에이(dna) 동정을 위한 방법 및 장치(methods and apparatus for dna sequencing and dna identification) - Google Patents

디엔에이(dna) 시퀀싱 및 디엔에이(dna) 동정을 위한 방법 및 장치(methods and apparatus for dna sequencing and dna identification)

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KR980700433A
KR980700433A KR1019970703847A KR19970703847A KR980700433A KR 980700433 A KR980700433 A KR 980700433A KR 1019970703847 A KR1019970703847 A KR 1019970703847A KR 19970703847 A KR19970703847 A KR 19970703847A KR 980700433 A KR980700433 A KR 980700433A
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nucleic acid
probes
probe
acid segments
hybridization
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라도제 티. 드르마낙
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그루버 루이스
하이세크, 인코포레이티드
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Abstract

본 발명은 다중 샘플의 다중 프로브 분석을 분리시키는 세분화된 필터를 채용하여 DNA 동정과 DNA 시퀀싱 용도로 사용될 수 있는, 하이브리디제이션에 의한 시퀀싱(SBH)방법 및 장치에 관한 것이다. 분할된 필터들은 제조되었다. 분할된 필터부에 샘플이 첨부되어, 각각의 색터가 하이브리디제이션 스코어링용으로 다중화된 프로브 또는 단일 프로브로 프로브된다. 하이브리디제에션 데이타는 프로브 완결성, SBH에 의한 부분 시퀀싱 또는 SBH에 의한 완전한 시퀀싱에 대해 분석된다.

Description

디엔에이(DNA) 시퀀싱 및 디엔에이(DNA) 동정을 위한 방법 및 장치(METHODS AND APPARATUS FOR DNA SEQUENCING AND DNA IDENTIFICATION)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (34)

  1. 기질의 제 1 섹터 상에 첫 번째 복수개의 핵산 세그먼트를 어레이시키고; 기질의 제 2 섹터 상에 두 번째 복수개의 핵산 세그먼트를 배치한 다음; 완전히 상보적인 것과 단일 염기 미스맷치를 구별할 수 있는 조건 하에서, 상기 제 1 섹터 중의 제 1 하이브리디제이션 프로브 (여기서 상기 제 1 하이브리디제이션 프로브는 상기 첫번째 복수개의 핵산 세그먼트 중 어느 하나 보다 짧음)에 첫 번째 복수개의 핵산 세그먼트를 노출시키고; 완전히 상보적인 것과 단일 염기 미스맷치를 구별할 수 있는 조건 하에서, 상기 제 2 섹터 중의 제 2 하이브리디제이션 프로브 (여기서 상기 제 2 하이브리디제이션 프로브는 상기 두번째 복수개의 핵산 세그먼트 중 어느 하나 보다 짧고 상기 첫 번째 복수개의 핵산 세그먼트와 상이함)를 인큐베이팅한 다음; 하이브리디제이션 프로브가 핵산 세그먼트에 하이브리디제이션 되었는지를 검색하여; 그 결과를 분석하는 단계로 이루어지는, 하이브리디제이션에 의한 핵산 분석 방법.
  2. 제 1항에 있어서, 상기 두 번째 복수개의 핵산 세그먼트의 배치 단계에 앞서, 핵산 이동을 막는 장벽을 도입하는 단계를 추가로 포함하는 방법.
  3. 제 1항에 있어서, 상기 첫 번째 핵산 세그먼트의 어레이 단계와 상기 두 번째 복수개의 핵산 세그먼트이 배치 단계 후, 상기 인큐베이션 단계 전에, 핵산 이동을 막는 장벽을 도입하는 단계를 추가로 포함하는 방법.
  4. 제 3항에 있어서, 상기 도입 단계가 물리적 장벽을 상기 기질에 대해 압착하는 단계인 방법.
  5. 제 2항에 있어서, 상기 도입 단계가 상기 지지체에 대해 수직인 방향-스위칭 전기장을 인가함으로써 섹터들 간의 프로브의 혼합을 방지하는 단계인 방법.
  6. 제 3항에 있어서, 상기 도입 단계가 상기 지지체에 대해 수직인 방향-스위칭 전기장을 인가함으로써 섹터들 간의 프로브의 혼합을 방지하는 단계인 방법.
  7. 제 1항에 있어서, 상기 어레이 단계가 핀 어레이 수단에 의해 핵산 샘플을 스포팅하는 단계로 이루어진 방법.
  8. 제 1항에 있어서, 상기 어레이 단계가 튜브 어레이에 의해 핵산 샘플을 배치하는 단계로 이루어진 방법.
  9. 제 1항에 있어서, 상기 어레이 단계가 핵산 샘플을 제트 프린팅하는 단계로 이루어진 방법.
  10. 제 1항에 있어서, 상기 노출 단계가 복수개의 인접하는 하이브리다이징 프로브들을 적용하는 단계로 이루어진 방법.
  11. 제 1항에 있어서, 상기 인큐베이팅 단계가 복수개의 인접하는 하이브리다이징 프로브들을 적용하는 단계로 이루어진 방법.
  12. 제 10항에 있어서, 2개 이상의 상기 복수개의 인접하는 하이브리다이징 프로브들을 라이게이팅시키는 단계를 출가로 포함하는 방법.
  13. 제 11항에 있어서, 2개 이상의 상기 복수개의 인접하는 하이브리다이징 프로브들을 라이게이팅시키는 단계를 출가로 포함하는 방법.
  14. 제 1항에 있어서, 상기 노출 단계가 중복되는 핵산 서열을 갖는 복수개의 경쟁적인 하이브리다이징 프로브들을 적용하는 단계로 이루어진 방법.
  15. 제 1항에 있어서, 상기 인큐베이선 단계가 중복되는 핵산 서열을 갖는 복수개의 경쟁적인 하이브리다이징 프로브들을 적용하는 단계로 이루어진 방법.
  16. 제 1항에 있어서, 2개 이상의 상기 첫 번째 복수개의 핵산 세그먼트들을 혼합물로서 배치하는 방법.
  17. 제 1항에 있어서, 2개 이상의 상기 두 번째의 복수개의 핵산 세그먼트들을 혼합물로서 배치하는 방법.
  18. 제 1항에 있어서, Hga I형 제한 효소를 이용한 절단과 그 결과 얻어진 제한 효소 절단된 단편들을 앵커를 이용하여 라이게이션시킴으로써 샘플을 제조하는 단계를 추가로 포함하는 방법.
  19. 제 1항에 있어서, 주어진 길이의 보편적인 프로브 세트로부터 프로브들을 선별하는 단계를 추가로 포함하는 방법.
  20. 제 1항에 있어서, 주어진 길이의 불완전한 프로브 세트로부터 프로브들을 선별하는 단계를 추가로 포함하는 방법.
  21. 제 1항에 있어서, 데옥시리보뉴클레오티드 프로브들을 선별하는 단계를 추가로 포함하는 방법.
  22. 제 1항에 있어서, 리보뉴클레오티드 프로브들을 선별하는 단계를 추가로 포함하는 방법.
  23. 제 1항에 있어서, 염기 동족체를 함유하는 프로브들과 단백질 핵산 프로브 그룹으로부터 핵산 동족체를 선별하는 단계를 추가로 포함하는 방법.
  24. 제 1항에 있어서, 프로브들을 다중 표지시키는 단계를 추가로 포함하는 방법.
  25. 제 1항에 있어서, 하이브리다이즈되지 않은 프로브 상의 표지를 분해시키는 단계를 추가로 포함하는 방법.
  26. 제 19항에 있어서, 상기 노출 단계 또는 상기 인큐베이팅 단계가 6, 7 8, 9 또는 10 염기 길이의 보편적인 프로브 세트를 어셈블리시키는 단계로 이루어진 방법.
  27. 제 19항에 있어서, 상기 노출 단계 또는 상기 인큐베이팅 단계가 6, 7 8, 9 또는 10 염기 길이의 보편적인 프로브 세트를 어셈블리시키는 단계로 이루어진 방법.
  28. 제 20항에 있어서, 상기 노출 단계 또는 상기 인큐베이팅 단계가 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 또는 30 염기 길이의 불완전 프로브 세트를 어셈블리시키는 단계로 이루어진 방법.
  29. 핵산 단편에 대한 접합접을 가지며, 소수성 부위에 의해 분할된 기질을 함유하는, 하이브리디제이션에 의한 핵산 분석 장치
  30. 제 20항에 있어서, 상기 배치 단계가 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 또는 30 염기 길이의 불완전 프로브 세트를 어셈블리시키는 단계로 이루어진 방법.
  31. 제 1항에 있어서, 2개 이상의 염기를 포함하는 중복된 핵산 서열을 갖는 2개 이상의 프로브들의 하이브리디제이션을 검정함으로써, 어떤 세그먼트 내의 2개 이상의 상기 염기들의 상대적인 순서를 확인하는 단계를 추가로 포함하는 방법.
  32. 샘플을 프로브 어레이에 도입하고; 주어진 어떠한 시간에서도, 다수의 샘플 분자들이 라이게이트된 프로브들과 회합되지 않도록 온도를 조정한 다음; 표지된 프로브를 혼합물에 첨가하고; 혼합물을 라이게이즈와 함께 인큐베이팅시킨 다음; 유리 프로브들을 제거하고; 라이게이션 생성물을 검색하는 단계들로 이루어진 뉴클레오티드 서열 분석 방법.
  33. 제 1항에 있어서, 원하는 결과를 개선시키기 위해 부가적인 프로브들을 정의하는 단계와 상기 노출, 인큐베이팅, 검색 및 분석 단계들을 반복하는 단계들을 추가로 포함하는 방법.
  34. 제 1항에 있어서, 상기 복수개의 핵산 세그먼트들을 재사용하기 위해 기질로부터 프로브들을 벗겨내는 단계를 추가로 포함하는 방법.
KR1019970703847A 1994-12-09 1995-12-08 디엔에이(dna) 시퀀싱 및 디엔에이(dna) 동정을 위한 방법 및 장치(methods and apparatus for dna sequencing and dna identification) Ceased KR980700433A (ko)

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Families Citing this family (233)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6270961B1 (en) * 1987-04-01 2001-08-07 Hyseq, Inc. Methods and apparatus for DNA sequencing and DNA identification
US6040138A (en) * 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US6919211B1 (en) * 1989-06-07 2005-07-19 Affymetrix, Inc. Polypeptide arrays
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US6955915B2 (en) * 1989-06-07 2005-10-18 Affymetrix, Inc. Apparatus comprising polymers
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US6551784B2 (en) 1989-06-07 2003-04-22 Affymetrix Inc Method of comparing nucleic acid sequences
US5925525A (en) * 1989-06-07 1999-07-20 Affymetrix, Inc. Method of identifying nucleotide differences
US6346413B1 (en) 1989-06-07 2002-02-12 Affymetrix, Inc. Polymer arrays
US6379895B1 (en) 1989-06-07 2002-04-30 Affymetrix, Inc. Photolithographic and other means for manufacturing arrays
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US6506558B1 (en) 1990-03-07 2003-01-14 Affymetrix Inc. Very large scale immobilized polymer synthesis
EP0834576B1 (en) * 1990-12-06 2002-01-16 Affymetrix, Inc. (a Delaware Corporation) Detection of nucleic acid sequences
US6468740B1 (en) 1992-11-05 2002-10-22 Affymetrix, Inc. Cyclic and substituted immobilized molecular synthesis
US6401267B1 (en) * 1993-09-27 2002-06-11 Radoje Drmanac Methods and compositions for efficient nucleic acid sequencing
US6287850B1 (en) * 1995-06-07 2001-09-11 Affymetrix, Inc. Bioarray chip reaction apparatus and its manufacture
US8236493B2 (en) * 1994-10-21 2012-08-07 Affymetrix, Inc. Methods of enzymatic discrimination enhancement and surface-bound double-stranded DNA
US5795716A (en) * 1994-10-21 1998-08-18 Chee; Mark S. Computer-aided visualization and analysis system for sequence evaluation
US5801155A (en) 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
US6312894B1 (en) 1995-04-03 2001-11-06 Epoch Pharmaceuticals, Inc. Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
US5830655A (en) 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US6720149B1 (en) * 1995-06-07 2004-04-13 Affymetrix, Inc. Methods for concurrently processing multiple biological chip assays
US6660233B1 (en) * 1996-01-16 2003-12-09 Beckman Coulter, Inc. Analytical biochemistry system with robotically carried bioarray
EP0880598A4 (en) 1996-01-23 2005-02-23 Affymetrix Inc RAPID EVALUATION OF NUCLEIC ACID ABUNDANCE DIFFERENCE, WITH A HIGH-DENSITY OLIGONUCLEOTIDE SYSTEM
CA2266745A1 (en) 1996-09-19 1998-03-26 Affymetrix, Inc. Identification of molecular sequence signatures and methods involving the same
US6391550B1 (en) * 1996-09-19 2002-05-21 Affymetrix, Inc. Identification of molecular sequence signatures and methods involving the same
US20020042048A1 (en) * 1997-01-16 2002-04-11 Radoje Drmanac Methods and compositions for detection or quantification of nucleic acid species
US6297006B1 (en) * 1997-01-16 2001-10-02 Hyseq, Inc. Methods for sequencing repetitive sequences and for determining the order of sequence subfragments
ATE296898T1 (de) 1997-03-20 2005-06-15 Affymetrix Inc Iterative resequenzierung
US20030036084A1 (en) * 1997-10-09 2003-02-20 Brian Hauser Nucleic acid detection method employing oligonucleotide probes affixed to particles and related compositions
US6322968B1 (en) 1997-11-21 2001-11-27 Orchid Biosciences, Inc. De novo or “universal” sequencing array
US7045610B2 (en) 1998-04-03 2006-05-16 Epoch Biosciences, Inc. Modified oligonucleotides for mismatch discrimination
US6949367B1 (en) 1998-04-03 2005-09-27 Epoch Pharmaceuticals, Inc. Modified oligonucleotides for mismatch discrimination
US6683173B2 (en) 1998-04-03 2004-01-27 Epoch Biosciences, Inc. Tm leveling methods
US7715989B2 (en) 1998-04-03 2010-05-11 Elitech Holding B.V. Systems and methods for predicting oligonucleotide melting temperature (TmS)
US6127121A (en) * 1998-04-03 2000-10-03 Epoch Pharmaceuticals, Inc. Oligonucleotides containing pyrazolo[3,4-D]pyrimidines for hybridization and mismatch discrimination
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6872521B1 (en) 1998-06-16 2005-03-29 Beckman Coulter, Inc. Polymerase signaling assay
US6703228B1 (en) 1998-09-25 2004-03-09 Massachusetts Institute Of Technology Methods and products related to genotyping and DNA analysis
EP1001037A3 (en) * 1998-09-28 2003-10-01 Whitehead Institute For Biomedical Research Pre-selection and isolation of single nucleotide polymorphisms
EP1121472B1 (en) * 1998-10-13 2006-01-04 Brown University Research Foundation Systems and methods for sequencing by hybridization
US7071324B2 (en) 1998-10-13 2006-07-04 Brown University Research Foundation Systems and methods for sequencing by hybridization
US7034143B1 (en) 1998-10-13 2006-04-25 Brown University Research Foundation Systems and methods for sequencing by hybridization
US6545264B1 (en) 1998-10-30 2003-04-08 Affymetrix, Inc. Systems and methods for high performance scanning
EP1144684B1 (en) 1999-01-06 2009-08-19 Callida Genomics, Inc. Enhanced sequencing by hybridization using pools of probes
US7595189B2 (en) 1999-01-08 2009-09-29 Applied Biosystems, Llc Integrated optics fiber array
JP4454155B2 (ja) 1999-01-08 2010-04-21 アプライド バイオシステムズ， エルエルシー 化学種を接触させるためのファイバーアレイならびにファイバーアレイを使用および作製する方法
CA2366289A1 (en) 1999-03-25 2000-09-28 Radoje T. Drmanac Solution-based methods and materials for sequence analysis by hybridization
US6516276B1 (en) * 1999-06-18 2003-02-04 Eos Biotechnology, Inc. Method and apparatus for analysis of data from biomolecular arrays
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) * 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6339147B1 (en) 1999-07-29 2002-01-15 Epoch Biosciences, Inc. Attachment of oligonucleotides to solid supports through Schiff base type linkages for capture and detection of nucleic acids
EP1235932A2 (en) 1999-10-08 2002-09-04 Protogene Laboratories, Inc. Method and apparatus for performing large numbers of reactions using array assembly
JP3668075B2 (ja) * 1999-10-12 2005-07-06 光夫 板倉 遺伝物質シーケンス決定用懸濁系、その懸濁系を用いた遺伝物質シーケンス決定方法およびその懸濁系を用いたＳＮＰｓ高速スコアリング方法
US7668658B2 (en) 1999-10-13 2010-02-23 Sequenom, Inc. Methods for generating databases and databases for identifying polymorphic genetic markers
US6660845B1 (en) 1999-11-23 2003-12-09 Epoch Biosciences, Inc. Non-aggregating, non-quenching oligomers comprising nucleotide analogues; methods of synthesis and use thereof
US20040081959A9 (en) 1999-12-08 2004-04-29 Epoch Biosciences, Inc. Fluorescent quenching detection reagents and methods
US6727356B1 (en) 1999-12-08 2004-04-27 Epoch Pharmaceuticals, Inc. Fluorescent quenching detection reagents and methods
US7205105B2 (en) 1999-12-08 2007-04-17 Epoch Biosciences, Inc. Real-time linear detection probes: sensitive 5′-minor groove binder-containing probes for PCR analysis
CA2396320A1 (en) * 2000-01-11 2001-07-19 Maxygen, Inc. Integrated systems and methods for diversity generation and screening
JP2003525292A (ja) 2000-03-01 2003-08-26 エポック・バイオサイエンシーズ・インコーポレイテッド ミスマッチ識別のための修飾オリゴヌクレオチド
EP1944310A3 (en) 2000-03-01 2008-08-06 Epoch Biosciences, Inc. Modified oligonucleotides for mismatch discrimination
JP3502803B2 (ja) * 2000-03-06 2004-03-02 日立ソフトウエアエンジニアリング株式会社 マイクロアレイ、マイクロアレイ作製方法及びマイクロアレイにおけるピン間スポット量誤差補正方法
WO2001092579A2 (en) * 2000-05-30 2001-12-06 Pe Corporation (Ny) Methods for detecting target nucleic acids using coupled ligation and amplification
DK1290444T3 (da) 2000-06-14 2010-01-18 Vistagen Inc Toxicitetstypisering under anvendelse af lever-stamceller
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US6958214B2 (en) 2000-07-10 2005-10-25 Sequenom, Inc. Polymorphic kinase anchor proteins and nucleic acids encoding the same
US6913879B1 (en) 2000-07-10 2005-07-05 Telechem International Inc. Microarray method of genotyping multiple samples at multiple LOCI
US6984522B2 (en) 2000-08-03 2006-01-10 Regents Of The University Of Michigan Isolation and use of solid tumor stem cells
US6681186B1 (en) 2000-09-08 2004-01-20 Paracel, Inc. System and method for improving the accuracy of DNA sequencing and error probability estimation through application of a mathematical model to the analysis of electropherograms
AU2002228974A1 (en) 2000-12-13 2002-06-24 Nugen Technologies, Inc Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
CN101310010A (zh) 2001-01-02 2008-11-19 斯坦姆荣公司 一种生产具有预先选择的免疫型和/或基因型的纯合干细胞群的方法,适于从其衍生的移植物的细胞,和使用它们的材料及方法
WO2003012147A1 (en) * 2001-02-20 2003-02-13 Datascope Investment Corp. Method for reusing standard blots and microarrays utilizing dna dendrimer technology
NZ523167A (en) 2001-03-09 2006-08-31 Nugen Technologies Inc Methods, compositions and kits for amplifying RNA sequences of interest which employ an RNA/DNA composite primer
WO2002072892A1 (en) 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
DE10120798B4 (de) * 2001-04-27 2005-12-29 Genovoxx Gmbh Verfahren zur Bestimmung der Genexpression
WO2002090599A1 (en) * 2001-05-09 2002-11-14 Genetic Id, Inc. Universal microarray system
US20030036073A1 (en) * 2001-06-07 2003-02-20 Saba James Anthony Matrix Sequencing: a novel method of polynucleotide analysis utilizing probes containing universal nucleotides
US6767731B2 (en) 2001-08-27 2004-07-27 Intel Corporation Electron induced fluorescent method for nucleic acid sequencing
WO2003020898A2 (en) * 2001-08-30 2003-03-13 Spectral Genomics, Inc. Arrays comprising pre-labeled biological molecules and methods for making and using these arrays
US20030059822A1 (en) * 2001-09-18 2003-03-27 U.S. Genomics, Inc. Differential tagging of polymers for high resolution linear analysis
US20030224380A1 (en) * 2001-10-25 2003-12-04 The General Hospital Corporation Genes and polymorphisms on chromosome 10 associated with Alzheimer's disease and other neurodegenerative diseases
US20030170678A1 (en) * 2001-10-25 2003-09-11 Neurogenetics, Inc. Genetic markers for Alzheimer's disease and methods using the same
AU2002364945A1 (en) * 2001-10-25 2003-07-09 Neurogenetics, Inc. Genes and polymorphisms on chromosome 10 associated with alzheimer's disease and other neurodegenerative diseases
GB0202462D0 (en) * 2002-02-04 2002-03-20 Tepnel Medical Ltd Nucleic acid analysis
WO2003093296A2 (en) * 2002-05-03 2003-11-13 Sequenom, Inc. Kinase anchor protein muteins, peptides thereof, and related methods
CA2486283A1 (en) * 2002-05-17 2004-02-05 Nugen Technologies, Inc. Methods for fragmentation, labeling and immobilization of nucleic acids
DE10224824A1 (de) * 2002-06-05 2003-12-24 Eppendorf Ag Verfahren zur Analyse von Target-Nukleinsäuresequenzen
EP2385138A1 (en) 2002-07-31 2011-11-09 University of Southern California Polymorphisms for predicting disease and treatment outcome
US20040235005A1 (en) * 2002-10-23 2004-11-25 Ernest Friedlander Methods and composition for detecting targets
US6641899B1 (en) * 2002-11-05 2003-11-04 International Business Machines Corporation Nonlithographic method to produce masks by selective reaction, articles produced, and composition for same
CA2555962C (en) 2003-02-26 2015-10-06 Callida Genomics, Inc. Random array dna analysis by hybridization
US20070141570A1 (en) * 2003-03-07 2007-06-21 Sequenom, Inc. Association of polymorphic kinase anchor proteins with cardiac phenotypes and related methods
FR2852317B1 (fr) 2003-03-13 2006-08-04 Biopuces a sondes et leurs methodes d'utilisation
ES2346881T3 (es) 2003-03-19 2010-10-21 The University Of British Columbia Haplotipos del inhibidor del activador del plasminogeno 1 (pai-1) utiles como indicadores de resultado del paciente.
US7393207B2 (en) * 2003-03-26 2008-07-01 Shin-Etsu Handotai Co., Ltd. Wafer support tool for heat treatment and heat treatment apparatus
WO2004092418A2 (en) 2003-04-14 2004-10-28 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
US8652774B2 (en) * 2003-04-16 2014-02-18 Affymetrix, Inc. Automated method of manufacturing polyer arrays
US7425700B2 (en) * 2003-05-22 2008-09-16 Stults John T Systems and methods for discovery and analysis of markers
WO2005001129A2 (en) * 2003-06-06 2005-01-06 Applera Corporation Mobility cassettes
US20050170367A1 (en) * 2003-06-10 2005-08-04 Quake Stephen R. Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
JP4067463B2 (ja) * 2003-07-18 2008-03-26 トヨタ自動車株式会社 ハイブリッド車輌の制御装置
US20050038776A1 (en) * 2003-08-15 2005-02-17 Ramin Cyrus Information system for biological and life sciences research
US7348146B2 (en) 2003-10-02 2008-03-25 Epoch Biosciences, Inc. Single nucleotide polymorphism analysis of highly polymorphic target sequences
PT1687609E (pt) 2003-10-28 2015-03-02 Epoch Biosciences Inc Sondas fluorescentes para a detecção de adn por hibridação com uma maior sensibilidade e menor ruído de fundo
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005049849A2 (en) 2003-11-14 2005-06-02 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use
US7276338B2 (en) * 2003-11-17 2007-10-02 Jacobson Joseph M Nucleotide sequencing via repetitive single molecule hybridization
US20060172408A1 (en) * 2003-12-01 2006-08-03 Quake Steven R Device for immobilizing chemical and biochemical species and methods of using same
CA2552007A1 (en) 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
EP1716254B1 (en) 2004-02-19 2010-04-07 Helicos Biosciences Corporation Methods for analyzing polynucleotide sequences
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
EP1725574A4 (en) 2004-03-04 2008-10-29 Univ British Columbia THROMBOMODULIN (THBD) HAPLOTYPES AS RESULTS INDICATORS FOR PATIENTS
GB2413796B (en) * 2004-03-25 2006-03-29 Global Genomics Ab Methods and means for nucleic acid sequencing
US20050239085A1 (en) * 2004-04-23 2005-10-27 Buzby Philip R Methods for nucleic acid sequence determination
US20050260609A1 (en) * 2004-05-24 2005-11-24 Lapidus Stanley N Methods and devices for sequencing nucleic acids
US20070117103A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
ATE507305T1 (de) 2004-05-25 2011-05-15 Helicos Biosciences Corp Verfahren zur nukleinsäureimmobilisierung
US20070117104A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US20060024678A1 (en) * 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
US8391959B2 (en) * 2004-09-29 2013-03-05 Tel Hashomer Medical Research Infrastructure And Services Ltd. Composition for improving efficiency of drug delivery
US20060118754A1 (en) * 2004-12-08 2006-06-08 Lapen Daniel C Stabilizing a polyelectrolyte multilayer
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US20060172328A1 (en) * 2005-01-05 2006-08-03 Buzby Philip R Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
CA2598510C (en) * 2005-02-18 2011-12-20 Abraxis Bioscience, Inc. Q3 sparc deletion mutant and uses thereof
US20060263790A1 (en) * 2005-05-20 2006-11-23 Timothy Harris Methods for improving fidelity in a nucleic acid synthesis reaction
EP1907560B1 (en) 2005-05-20 2013-01-23 Integrated DNA Technologies, Inc. Compounds and methods for labeling oligonucleotides
EP2463386B1 (en) * 2005-06-15 2017-04-12 Complete Genomics Inc. Nucleic acid analysis by random mixtures of non-overlapping fragments
EP1913155A4 (en) * 2005-06-23 2009-08-12 Univ British Columbia COAGULATION FACTOR III POLYMORPHISMS ASSOCIATED WITH PREDICTION RELATING TO THE RESULTS AND RESPONSE OF A SUBJECT TO THERAPY
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7939258B2 (en) 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
US7960104B2 (en) 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
US20070117102A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070128610A1 (en) * 2005-12-02 2007-06-07 Buzby Philip R Sample preparation method and apparatus for nucleic acid sequencing
WO2007091077A1 (en) 2006-02-08 2007-08-16 Solexa Limited Method for sequencing a polynucleotide template
SG170028A1 (en) * 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
CN101432439B (zh) * 2006-02-24 2013-07-24 考利达基因组股份有限公司 Dna阵列上的高通量基因组测序
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
WO2008005459A2 (en) * 2006-06-30 2008-01-10 Nugen Technologies, Inc. Methods for fragmentation and labeling of nucleic acids
GB0618514D0 (en) * 2006-09-20 2006-11-01 Univ Nottingham Trent Method of detecting interactions on a microarray using nuclear magnetic resonance
US20100130374A1 (en) * 2006-09-29 2010-05-27 Annuska Maria Glas High-throughput diagnostic testing using arrays
US7910354B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
WO2008070375A2 (en) 2006-11-09 2008-06-12 Complete Genomics, Inc. Selection of dna adaptor orientation
US20090105961A1 (en) * 2006-11-09 2009-04-23 Complete Genomics, Inc. Methods of nucleic acid identification in large-scale sequencing
EP2312024A1 (en) 2006-11-15 2011-04-20 The University Of British Columbia Polymorphisms predictive of anthracycline-induced cardiotoxicity
US8278061B2 (en) 2007-01-18 2012-10-02 University Of Southern California Polymorphisms in the EGFR pathway as markers for cancer treatment
US8435752B2 (en) * 2007-01-18 2013-05-07 University Of Southern California Gene polymorphisms predictive for dual TKI therapy
US7881933B2 (en) * 2007-03-23 2011-02-01 Verizon Patent And Licensing Inc. Age determination using speech
US7572990B2 (en) * 2007-03-30 2009-08-11 Intermec Ip Corp. Keypad overlay membrane
KR102069498B1 (ko) 2007-09-28 2020-01-23 포톨라 파마슈티컬스, 인코포레이티드 인자 Ｘａ 저해제에 대한 안티도트 및 그것을 사용하는 방법
US8278047B2 (en) 2007-10-01 2012-10-02 Nabsys, Inc. Biopolymer sequencing by hybridization of probes to form ternary complexes and variable range alignment
WO2009052214A2 (en) * 2007-10-15 2009-04-23 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
US8518640B2 (en) * 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US7897344B2 (en) * 2007-11-06 2011-03-01 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs
WO2009061840A1 (en) * 2007-11-05 2009-05-14 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation
WO2009059430A1 (en) * 2007-11-07 2009-05-14 The University Of British Columbia Microfluidic device and method of using same
WO2009073629A2 (en) 2007-11-29 2009-06-11 Complete Genomics, Inc. Efficient shotgun sequencing methods
BRPI0819018A2 (pt) 2007-11-30 2015-05-05 Bryan P Scheneider "métodos para determinar se um paciente possui risco aumentado de desenvolver hipertensão, para determinar se um paciente possui maior probalidade de se beneficiar do tratamento com um antagonista de vegf e kits para determinar se um paciente possui risco aumentado de desenvolver hipertensão e para determinar se um paciente possui maior probabilidade de se beneficiar do tratamento com um antagonista de vegf"
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
WO2009094583A1 (en) * 2008-01-23 2009-07-30 Complete Genomics, Inc. Methods and compositions for preventing bias in amplification and sequencing reactions
WO2009097368A2 (en) 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US7846666B2 (en) 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
US20090270273A1 (en) * 2008-04-21 2009-10-29 Complete Genomics, Inc. Array structures for nucleic acid detection
JP4667490B2 (ja) * 2008-07-09 2011-04-13 三菱電機株式会社 加熱調理器
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
US9650668B2 (en) 2008-09-03 2017-05-16 Nabsys 2.0 Llc Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
WO2010028140A2 (en) 2008-09-03 2010-03-11 Nabsys, Inc. Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
EP2344678A4 (en) * 2008-09-05 2012-06-06 Life Technologies Corp METHODS AND SYSTEMS FOR VALIDATION, CALIBRATION AND NORMALIZATION OF NUCLEIC ACID SEQUENCING
JP5701216B2 (ja) 2008-11-05 2015-04-15 ジェネンテック， インコーポレイテッド 加齢黄斑変性における遺伝的多型
WO2010096696A2 (en) * 2009-02-20 2010-08-26 Cold Spring Harbor Laboratory Harnessing high throughput sequencing for multiplexed specimen analysis
EP2411505A4 (en) 2009-03-26 2013-01-30 Univ California MESENCHYMAL STEM CELLS FOR PRODUCING HEMDERING RNA FOR SICKNESS MODIFICATION
PT3604510T (pt) 2009-03-30 2025-06-24 Alexion Pharma Inc Antídotos para inibidores do fator xa e métodos de utilização dos mesmos
WO2010123626A1 (en) 2009-04-24 2010-10-28 University Of Southern California Cd133 polymorphisms and expression predict clinical outcome in patients with cancer
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
WO2011005598A1 (en) 2009-06-24 2011-01-13 University Of Southern California Compositions and methods for the rapid biosynthesis and in vivo screening of biologically relevant peptides
WO2011008885A1 (en) 2009-07-15 2011-01-20 Portola Pharmaceuticals, Inc. Unit dose formulation of antidotes for factor xa inhibitors and methods of using the same
BR112012009408B1 (pt) 2009-10-21 2020-05-26 Genentech, Inc Métodos para predizer se um paciente com degeneração macular relacionada à idade (dmri) úmida tem maior probabilidade de se beneficiar pelo tratamento com um anticorpo anti-vegf de alta afinidade ou anticorpo anti-vegf
WO2011084757A1 (en) 2009-12-21 2011-07-14 University Of Southern California Germline polymorphisms in the sparc gene associated with clinical outcome in gastric cancer
WO2011085334A1 (en) 2010-01-11 2011-07-14 University Of Southern California Cd44 polymorphisms predict clinical outcome in patients with gastric cancer
US9323888B2 (en) 2010-01-19 2016-04-26 Verinata Health, Inc. Detecting and classifying copy number variation
US9260745B2 (en) 2010-01-19 2016-02-16 Verinata Health, Inc. Detecting and classifying copy number variation
US20120100548A1 (en) 2010-10-26 2012-04-26 Verinata Health, Inc. Method for determining copy number variations
US10388403B2 (en) 2010-01-19 2019-08-20 Verinata Health, Inc. Analyzing copy number variation in the detection of cancer
WO2011091063A1 (en) * 2010-01-19 2011-07-28 Verinata Health, Inc. Partition defined detection methods
GB2485644B (en) 2010-01-19 2012-11-21 Verinata Health Inc Improved identification of chromosomal aneuploidies using a normalising sequence
WO2011103467A2 (en) 2010-02-19 2011-08-25 Life Technologies Corporation Methods and systems for nucleic acid sequencing validation, calibration and normalization
US9506057B2 (en) 2010-03-26 2016-11-29 Integrated Dna Technologies, Inc. Modifications for antisense compounds
WO2011120049A1 (en) 2010-03-26 2011-09-29 Integrated Dna Technologies, Inc. Methods for enhancing nucleic acid hybridization
WO2012033848A1 (en) 2010-09-07 2012-03-15 Integrated Dna Technologies, Inc. Modifications for antisense compounds
EP2616554A1 (en) 2010-09-17 2013-07-24 President and Fellows of Harvard College Functional genomics assay for characterizing pluripotent stem cell utility and safety
US8715933B2 (en) 2010-09-27 2014-05-06 Nabsys, Inc. Assay methods using nicking endonucleases
EP2640849B1 (en) 2010-11-16 2016-04-06 Nabsys 2.0 LLC Methods for sequencing a biomolecule by detecting relative positions of hybridized probes
WO2012068519A2 (en) 2010-11-19 2012-05-24 Sirius Genomics Inc. Markers associated with response to activated protein c administration, and uses thereof
WO2012109574A2 (en) 2011-02-11 2012-08-16 Nabsys, Inc. Assay methods using dna binding proteins
US8969003B2 (en) 2011-03-23 2015-03-03 Elitech Holding B.V. Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use
EP2689031B1 (en) 2011-03-23 2016-10-19 ELITechGroup B.V. Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use
CN106319047B (zh) 2011-04-12 2020-02-18 维里纳塔健康公司 使用多态计数来解析基因组分数
US9411937B2 (en) 2011-04-15 2016-08-09 Verinata Health, Inc. Detecting and classifying copy number variation
CN109517897A (zh) 2011-04-28 2019-03-26 南加利福尼亚大学 人类髓源抑制性细胞癌症标记
ES2537189T3 (es) 2011-05-24 2015-06-03 Elitech Holding B.V. Detección de estafilococos áureos resistentes a la meticilina
SG11201401628WA (en) 2011-10-19 2014-05-29 Nugen Technologies Inc Compositions and methods for directional nucleic acid amplification and sequencing
RU2622082C2 (ru) 2011-11-14 2017-06-09 Нестек С.А. Анализы и способы выбора режима лечения пациента с депрессией
GB2533882B (en) 2012-01-26 2016-10-12 Nugen Tech Inc Method of enriching and sequencing nucleic acids of interest using massively parallel sequencing
WO2013119923A1 (en) 2012-02-09 2013-08-15 The Regents Of The University Of Michigan Different states of cancer stem cells
CN104619894B (zh) 2012-06-18 2017-06-06 纽亘技术公司 用于非期望核酸序列的阴性选择的组合物和方法
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
GB201220924D0 (en) 2012-11-21 2013-01-02 Cancer Res Inst Royal Materials and methods for determining susceptibility or predisposition to cancer
US9914966B1 (en) 2012-12-20 2018-03-13 Nabsys 2.0 Llc Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation
US10294516B2 (en) 2013-01-18 2019-05-21 Nabsys 2.0 Llc Enhanced probe binding
EP2971086A1 (en) 2013-03-14 2016-01-20 ELITechGroup B.V. Functionalized 3-alkynyl pyrazolopyrimidine analogues as universal bases and methods of use
US20140274738A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
US20160186263A1 (en) 2013-05-09 2016-06-30 Trustees Of Boston University Using plexin-a4 as a biomarker and therapeutic target for alzheimer's disease
JP6697380B2 (ja) 2013-06-10 2020-05-20 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 多能性幹細胞の有用性および安全性を特徴付けるための初期発生ゲノムアッセイ
CA2924277A1 (en) 2013-10-18 2015-04-23 Institut De Cardiologie De Montreal Genotyping tests and methods for evaluating plasma creatine kinase levels
JP6525473B2 (ja) 2013-11-13 2019-06-05 ニューゲン テクノロジーズ， インコーポレイテッド 複製物配列決定リードを同定するための組成物および方法
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
IL300472B2 (en) 2014-07-30 2024-11-01 Hoffmann La Roche Genetic markers for predicting responsiveness to therapy with hdl-raising or hdl mimicking agent
US9789087B2 (en) 2015-08-03 2017-10-17 Thomas Jefferson University PAR4 inhibitor therapy for patients with PAR4 polymorphism
WO2018226602A1 (en) 2017-06-05 2018-12-13 Research Institute At Nationwide Children's Hospital Enhanced modified viral capsid proteins
WO2019010410A1 (en) * 2017-07-07 2019-01-10 Massachusetts Institute Of Technology SYSTEMS AND METHODS OF GENETIC IDENTIFICATION AND ANALYSIS
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
WO2020146253A1 (en) * 2019-01-07 2020-07-16 Personal Genome Diagnostics Inc. Quantification of sequencing instruments and reagents for use in molecular diagnostic methods
CN109801679B (zh) * 2019-01-15 2021-02-02 广州柿宝生物科技有限公司 一种用于长链分子的数学序列重建方法
CA3146525A1 (en) 2019-08-05 2021-02-11 William Manning Systems and methods for sample preparation, data generation, and protein corona analysis
US12059674B2 (en) 2020-02-03 2024-08-13 Tecan Genomics, Inc. Reagent storage system
EP4208150A4 (en) 2020-09-03 2024-04-24 Dalan Animal Health Inc. Use of vitellogenin for defining and testing novel immunogens in insects
EP4430199A2 (en) 2021-11-24 2024-09-18 Research Institute at Nationwide Children's Hospital Chimeric hsv expressing hil21 to boost anti-tumor immune activity

Family Cites Families (75)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4302204A (en) * 1979-07-02 1981-11-24 The Board Of Trustees Of Leland Stanford Junior University Transfer and detection of nucleic acids
US4562159A (en) * 1981-03-31 1985-12-31 Albert Einstein College Of Medicine, A Division Of Yeshiva Univ. Diagnostic test for hepatitis B virus
CA1180647A (en) * 1981-07-17 1985-01-08 Cavit Akin Light-emitting polynucleotide hybridization diagnostic method
FI63596C (fi) * 1981-10-16 1983-07-11 Orion Yhtymae Oy Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser
US4591567A (en) 1982-04-21 1986-05-27 California Institute Of Technology Recombinant DNA screening system including fixed array replicator and support
JPS5927900A (ja) * 1982-08-09 1984-02-14 Wakunaga Seiyaku Kk 固定化オリゴヌクレオチド
DE3486488T2 (de) * 1983-01-10 2003-04-03 Gen-Probe Inc., San Diego Verfahren zum Nachweis, Identifizieren und Quantifizieren von Organismen und Viren
JPS6010174A (ja) * 1983-06-29 1985-01-19 Fuji Photo Film Co Ltd オ−トラジオグラフイ−による遺伝子のスクリ−ニング方法
CA1222680A (en) * 1983-07-05 1987-06-09 Nanibhushan Dattagupta Testing dna samples for particular nucleotide sequences
US4677054A (en) * 1983-08-08 1987-06-30 Sloan-Kettering Institute For Cancer Research Method for simple analysis of relative nucleic acid levels in multiple small samples by cytoplasmic dot hybridization
EP0155950B1 (en) * 1983-09-02 1990-01-17 Molecular Biosystems, Inc. Oligonucleotide polymeric support system
US4613566A (en) * 1984-01-23 1986-09-23 President And Fellows Of Harvard College Hybridization assay and kit therefor
FI71768C (fi) * 1984-02-17 1987-02-09 Orion Yhtymae Oy Foerbaettrade nykleinsyrareagenser och foerfarande foer deras framstaellning.
CA1223222A (en) 1984-02-22 1987-06-23 Nanibhushan Dattagupta Immobilized nucleic acid-containing probes
US4766062A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor
US5242794A (en) 1984-12-13 1993-09-07 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4883750A (en) 1984-12-13 1989-11-28 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
GB8432118D0 (en) * 1984-12-19 1985-01-30 Malcolm A D B Sandwich hybridisation technique
DE3506703C1 (de) * 1985-02-26 1986-04-30 Sagax Instrument AB, Sundbyberg Verfahren zur Sequenzanalyse von Nucleinsaeuren,insbesondere der Desoxyribonucleinsaeure (DNA) und der Ribonucleinsaeure (RNA),sowie Traeger zur Durchfuehrung des Verfahrens und Verfahren zur Herstellung des Traegers
GB8509880D0 (en) * 1985-04-17 1985-05-22 Ici Plc Testing device
EP0200113A3 (en) * 1985-04-30 1987-03-18 Pandex Laboratories, Inc. A method of solid phase nucleic acid hybridization assay incorporating a luminescent label
AU558846B2 (en) * 1985-06-21 1987-02-12 Miles Laboratories Inc. Solid-phase hydridization assay using anti-hybrid antibodies
US4794073A (en) * 1985-07-10 1988-12-27 Molecular Diagnostics, Inc. Detection of nucleic acid hybrids by prolonged chemiluminescence
US4775631A (en) * 1985-07-26 1988-10-04 Janssen Pharmaceuitica, N.V. Method of localizing nucleic acids bound to polyamide supports
US4806546A (en) * 1985-09-30 1989-02-21 Miles Inc. Immobilization of nucleic acids on derivatized nylon supports
US4806631A (en) * 1985-09-30 1989-02-21 Miles Inc. Immobilization of nucleic acids on solvolyzed nylon supports
TW203120B (ko) * 1985-10-04 1993-04-01 Abbott Lab
US4770992A (en) * 1985-11-27 1988-09-13 Den Engh Gerrit J Van Detection of specific DNA sequences by flow cytometry
US4882269A (en) * 1985-12-13 1989-11-21 Princeton University Amplified hybridization assay
EP0228075B1 (en) * 1986-01-03 1991-04-03 Molecular Diagnostics, Inc. Eucaryotic genomic dna dot-blot hybridization method
EP0231010A3 (en) * 1986-01-27 1990-10-17 INCSTAR Corporation A method of solid phase enzyme immunoassay and nucleic acid hybridization assay and dip-stick design and stabilized chromogenic substrate
NO870613L (no) * 1986-03-05 1987-09-07 Molecular Diagnostics Inc Deteksjon av mikroorganismer i en prŸve inneholdende nukleinsyre.
US5348855A (en) * 1986-03-05 1994-09-20 Miles Inc. Assay for nucleic acid sequences in an unpurified sample
CA1284931C (en) * 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
US5310893A (en) * 1986-03-31 1994-05-10 Hoffmann-La Roche Inc. Method for HLA DP typing
AU7015287A (en) * 1986-03-19 1987-09-24 Cetus Corporation Detection of nucleic acid sequence using liquid hybridization method
US4981783A (en) * 1986-04-16 1991-01-01 Montefiore Medical Center Method for detecting pathological conditions
EP0245206A1 (en) * 1986-05-05 1987-11-11 IntraCel Corporation Analytical method for detecting and measuring specifically sequenced nucleic acid
ATE88762T1 (de) * 1986-08-11 1993-05-15 Siska Diagnostics Inc Methoden und zusammensetzungen fuer tests mit nukleinsaeuresonden.
DE3850273T2 (de) * 1987-03-02 1994-09-29 Gen Probe Inc Polykationische Träger zur Reinigung, Trennung und Hybridisierung von Nukleinsäure.
US4885250A (en) * 1987-03-02 1989-12-05 E. I. Du Pont De Nemours And Company Enzyme immobilization and bioaffinity separations with perfluorocarbon polymer-based supports
AU601021B2 (en) * 1987-03-11 1990-08-30 Molecular Diagnostics, Inc. Assay for necleic acid sequences in a sample
US6270961B1 (en) * 1987-04-01 2001-08-07 Hyseq, Inc. Methods and apparatus for DNA sequencing and DNA identification
US5525464A (en) * 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
IL85551A0 (en) * 1987-04-01 1988-08-31 Miles Inc Rapid hybridization assay and reagent system used therein
US4849334A (en) * 1987-06-09 1989-07-18 Life Technologies, Inc. Human papillomavirus 43 nucleic acid hybridization probes and methods for employing the same
JPH02503983A (ja) * 1987-06-26 1990-11-22 イー・アイ・デュポン・ドゥ・ヌムール・アンド・カンパニー 捕捉ビーズを用いる組み換えクローン化ｎａからの混入配列のアフイニテイ除去
US5120643A (en) * 1987-07-13 1992-06-09 Abbott Laboratories Process for immunochromatography with colloidal particles
US4921805A (en) * 1987-07-29 1990-05-01 Life Technologies, Inc. Nucleic acid capture method
US4942124A (en) * 1987-08-11 1990-07-17 President And Fellows Of Harvard College Multiplex sequencing
EP0305145A3 (en) * 1987-08-24 1990-05-02 Ortho Diagnostic Systems Inc. Methods and probes for detecting nucleic acids
EP0322311B1 (en) * 1987-12-21 1994-03-23 Applied Biosystems, Inc. Method and kit for detecting a nucleic acid sequence
US5354657A (en) * 1988-01-12 1994-10-11 Boehringer Mannheim Gmbh Process for the highly specific detection of nucleic acids in solid
GB8810400D0 (en) * 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
US4988617A (en) * 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
US5002867A (en) * 1988-04-25 1991-03-26 Macevicz Stephen C Nucleic acid sequence determination by multiple mixed oligonucleotide probes
DE68928853T2 (de) * 1988-05-20 1999-08-05 Cetus Corp., Emeryville, Calif. Befestigung von sequenzspezifischen proben
US5094939A (en) * 1988-07-19 1992-03-10 Fujirebio, Inc. Chemiluminescence assays using stabilized dioxetane derivatives
GB8822228D0 (en) * 1988-09-21 1988-10-26 Southern E M Support-bound oligonucleotides
JPH02299598A (ja) * 1989-04-14 1990-12-11 Ro Inst For Molecular Genetics & Geneteic Res 微視的サイズの別個の粒子と結合している核酸試料中のごく短い配列の全部または一部のオリゴヌクレチドプローブとのハイブリダイゼーションによる決定法
US5424186A (en) 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
JPH06504427A (ja) * 1990-10-17 1994-05-26 ジェン・トラック・インコーポレーテッド 多重核酸配列の存在または不存在の検出による識別および父性決定
EP0834576B1 (en) * 1990-12-06 2002-01-16 Affymetrix, Inc. (a Delaware Corporation) Detection of nucleic acid sequences
WO1992020824A1 (en) * 1991-05-24 1992-11-26 Walter Gilbert Method and apparatus for rapid nucleic acid sequencing
JPH07501929A (ja) * 1991-08-23 1995-03-02 アイシス・ファーマシューティカルス・インコーポレーテッド オリゴマーフラグメントの合成非ランダム化
US5474796A (en) 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
US6017696A (en) 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
US5503980A (en) * 1992-11-06 1996-04-02 Trustees Of Boston University Positional sequencing by hybridization
NZ275194A (en) * 1993-09-27 1997-09-22 Arch Dev Corp Method and kit for nucleic acid sequencing
CA2186465A1 (en) * 1994-04-04 1995-10-12 Richard A. Martinelli Hybridization-ligation assays for the detection of specific nucleic acid sequences
GB9507238D0 (en) * 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
US5545531A (en) * 1995-06-07 1996-08-13 Affymax Technologies N.V. Methods for making a device for concurrently processing multiple biological chip assays
EP0937159A4 (en) * 1996-02-08 2004-10-20 Affymetrix Inc SPECIATION OF MICROORGANISMS FROM MICROPLATES AND CHARACTERIZATION OF THE PHENOTYPES THEREOF

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