PT105731B - USE OF NATURAL ANTIOXIDANT AND ANTIPROLIFERATIVE PRODUCTS OF ALFARROBA BIOMASS - Google Patents

Abstract

The present invention relates to the application of a liquid extract consisting of natural products obtained from pollen from Alfarroba (for example, the polar from Alfarrubia, the byproduct of Alfarrubia), for use in the prevention / treatment of diseases PROLIFERATIVAS. The liquid extract is characterized by being simultaneously anti-oxidizing and anti-proliferative, consisting of specific phenolic compounds with bioactive properties.

Description

USE OF NATURAL ANTIOIDANT PRODUCTS AND

PREPARATIONS OF ALEXRORA BXOMEDDA »

The present invention d.

Iquido-ethoid as a pulp noidon from 1 to 3 ob ob cited:; respect to apl pdd · produdes a 1 da. r wheel wheel by-product carob processing _; for the prevention / treatment of liquid extant diseases: oaraoterisan by autosexidants and andipoliphorous agents: the use of natural exfoliates obtained from exare; lo, the pulp of the industry to be useful in the ratification. It is only the same as the cup that has specific bio-active properties.

This net extract asks ssr st 111, aedo eu nu nu 1 industrial actions s _? ndmeadãmeute to Inoustrus s. 1 cosmetic pharmaceuticals.

DESCRIPTION '' UTILIZATION OF NATURAL PRODUCTS AETXOXXDANT B

ANTIPRODTFBRATXVOS UR BUTTERFLY

I presume? .1 The invention concerns the application of a ccusLi.tulco liquid extract by natural products to be used for the prevention / treatment of prolitaceous diseases, from locust bean residues or by-products such as carob seed removal industry, also called carob biomass. These residues are subjected to aqueous extraction (in the presence of oil-solvents in a ratio of 10 to 10 liters in water) assisted by ultrasound to yield a bioactive liquid extract formed essentially of compounds containing 1 to 2 s. da a t.1 v i. da da f i i ona 1.

BETWEEN INVESTIGATION IS RESI.dnoa: Carob pulp (Cerafonia sfllqaa IA are the by-products of carob industrialization, notably the use of the seed. They are in good health: stAll: na: aliwutagão siuimal, but also:: s the confection of the regional confectionery from the flour, which is obtained by grinding it after the roasting of the pod pulp.

These carob residues have been used as a potential source of compounds or a biolege activity dm, s - ú], ie. t in o u mp r 1 d ο ρ o r lack of a prcoeseo: and spun a to obtain them.

For example, dua Lufei references refer to utii.i ration of ext anointing supposing criticism always implies one. initial removal of j sugars, drying © milling waste

Carob: What is the process? In addition, the extraction yield is not high. Gm among recurve work refers to the ation of ball-assisted extraction sound as a Ge? extract the smooth yield of the highest yielding aliarrabic pulp,

also extra extra appreciable amount of sugars » The present invention ? differs from state of the art. referred to in © Lfel, for not having limitations on the state of the raw material

initial cousin <From lacto, the present intent can coke raw materials any by-products of carob galga, irrespective of their qr anui ornei: ri © and humi Oade ..

To the saint? laves çl it differs from the state of the art pus: ss? refer to utilifefe of net extinction consisting of

natural products, antioxldamfe o © ntiprollferat amo da b a b a s a d a f r oPa ?, e.s χάνο ia ?: iman t a con s t 1 f do i do by po po po uni lices: gioaçti. wp gara texpeutele use i © prevention and / or aratamen:. © of prolific iterative diseases.

Is the present invention different from the prior art in using a useful ultrasonic extraction process? S ol vant fesíM? ^? '' iSurwrailv Re n oani sa d és úafe ?; _What is the particular advantage of feoutoo-eocfemmcus, with gains? of efficiency. and income., and rate the composition

f e node11oa and spa clf cr $. of products <

U u ti .7. Extraction systems such as the flowchart which are described in: 4-5, 11 are also advantageous because they are either industrial scale or have properties. It is the potential to degrade some of the present compounds, such as: phenoic®,: po-r mt 11 Ata: ® · in tempera tu rae tirite collated. In addition, extraction and consumption times are solved by asnaImanta alavad®.

The present invention differs from said prior art DC to ultrasonic extraction [dl per utillcar osoltardes '77 .AU ^f '' juntamante hundred Igua in the extraction process, which gives the product obtained in terms of different caracterist1cãs. fsebllca composition with blnmctir gone from,

There are very few works of the art relating to the antiproliferative activity of locust bean products. The present invention differs from the state of the art as regards its antiprolitic activity. This invention utilizes the crushed locust bean pulp and its residues against the ever-mentioned uplift of leaves, pods, farruha, cuadada da mamaurcie e®g, and the present invention also differs from the state of the art in that it reveals the first extract. of the 'prima specific matter' with iterative anti-suicidal suicide. over different cell lines, naming, human cells

H1F-7 and HeLa, and UEN dl roblastornas E-115 rats, Liem d®ssc · descrltcs estreies in the present invention are non - toxic mbcldos using solrent.es (water, acetone and c cauoà, _C®.: salsrnra® of these gnu foot rm.it in keeping de®: cleaning pPenó.ra 1 ay

Eeoagri sád As use; other sales: nt _: ®liÂdos n nm state of the art, noíseadanen <. and methanol, which provides these self-oxidizing and anti-proliferative natural products with the possibility of being directly and directly used. Both the extraction proses® and the solvents used do not differ either in composition or in the composition, either quantitative or quantitative, which innovative results of the present invention differed from the state of the extraction or solvents. They also give the products obtained the possibility of being used in industrial formulations, namely: 100: 00: 10,1.0: 0 to: l FOR DEVELOPMENT OF FUNCTIONAL FOODS, ®.> IN THE FORM OF THE SUPPLEMENTARY FOOD OR. Still as a drug of natural origin, it is an object of the present invention to use: a. liquid extract consisting of natural products :, s, i: Au 11 ana ament. and prior to 1: antitrusting for: therapeutic use in the prevention and / or treatment of proliferative diseases, obtained by: a treatment method comprising the following steps;

The; selection of bisetassa and raw material;

you healthy. its temperature: blossossa with water or: water with όρη o 1 v e u t o .Q Frogs;

(o) Placing the mixture into a banned sound mixer. t rolled;

d) pressing and filtering the liquid.

e) evaporating and / or dispensing the liquid extract;

Obtaining natural products by ilophilization of the saccharide: In the preferred embodiment of the present invention, the method of production utilizes the residues of locust bean pulp, a by-product of the locust bean processing industry. use of the seed and / or residues of itchy nests 1ndust r1a 1.

Switching to another preferred embodiment of the present invention, the method of obtaining utilizes crushed locust bean pulp.

In a preferred embodiment of the present invention, the method of production utilizes an ultrasonic bath mixture which is effected at a temperature of 20 ° C or above ^; ''W.

In yet another preferred embodiment of the present invention, the method of obtaining utilizes an ultrasonic mixing time of greater than 15 minutes.

In yet another real embodiment of the invention, the method of production utilizes a ratio st: lio © l, which ranges from 1: 1 to IrBQ (mvvg © afana: a mixture of water with c). 00-solvent.

burns: Preferred embodiment of the invention, the solvent used is acetone or sulfur in a ratio of from 10 to 051 percent of solvent in water.

This is a method of invention.

The preferred embodiment of the present invention is a step when the mixture is added and the evaporation of the co-solvent is combined with the solvent and the solvents.

It is further object of the proseais: invention to describe the liquid exxe that οοορνοοηοο saseuoialosnxe οοοροηχοο pol x fonél 1: ®.

In yet another embodiment of the present invention, the liquid extract has phenolic compounds such as the liquid, the gallium, the sulfur, and the mixtures thereof.

Puma Reaii. Preferred embodiment of the present invention. The liquid form exhibits anti-oxidant activity and self-proliferative activity.

It is still objective ®. The present invention describes the extrusion of the obtained lysine _: pn.Ic process described in formulations for the rare use industries, cos: stroan / on. to 1.1 ms © tar.

Background and Objective of the Present Invention: Use of Water and Additives of the Gnu Producto Neutrally Anti-Proliferative. gare therapeutic use in prevention ·?; © t. treatment of proliferative diseases, ordos qx. An extraction process is applied to the ultrasonelated aqueous extraction in the presence or absence of co-sellers to the others and to the carob pulp residues. These brisket materials are subjected to the extraction of sesianide by an ultrasound to obtain a liquid extract with. high corgo * ·, the phenolics, and the newly extracted solid nm, also: cam high compost * osuoa ... l v. · 'antifreezes: pr-esauoe® no exara too òquado <

The present invention, with its own self-oxidizing and anti-proliferative properties, can be used in industrial formulations without

need O a poses a r1or ex t r motion ass for fiction, the method and the method: st o r s 1 o d® s t z i s to feed, single pharmaceutical. prayed from: ecstm static.

Bmw Description Of® Figures

Claims (1)

For a more flexible invention of the invention, add *. Annex A: Finenesses, which represent preferred embodiments of the invention, which, however, are not intended to limit the scope of the present invention. Figure 1 depicts a chromatographic profile of the aqueous liquid extracted from the king's profile by chroming the strand of the atomic acid. The present invention relates to the use of a liquid and dry extractor by rotary products, although it is not possible to use a non-self-propelled tooth: for therapeutic purposes in the production and treatment of the product. / or treatment of prol if ® disease: addis? However, these were affected by the extraction of water by assisting ultra-aqueous assisted extraction in the presence or absence of Guas' oo-aolvant with ethanol or oysters to the carob pulp residues. These raw materials are subjected to a nitrogen-assisted extraction process to obtain a crude extract with the same content as fission compounds to the remains of the northwestern extract, also with a high content of different oils and compounds from those present in the liquid extract. . The liquid extract and the remaining extracted solid resulting from this product are its nourishing products containing the natural bile products which contain the polyphenolic packages; It may be directly used in industrial formulations: • To the west the auras present the following rules: sus çs re i. to products if: o 1.hanfes ex 1 watch out; beneficial for assisted harvesting of live residues: from the industry to the locust of the carob prigana products consisting of phenolic compounds, namely the ladies with acidic flavor to the palate, among the ::: · .: These compounds give the liquid extract thus obtained from the crushed carob pulp one. significant activity a n t r o x i d a n. t e a t i v 1 d a d a ant i p r o 1 i. fer t t vac on trace 1 u 1 the bumasas o aporapore: Pa marta dóbm from the song of eol © de stu.ro dcss, and against cells of PIEllâ rat rat insects, indicating that this biomass is a promising source of what we do as you do i.geuos Ford and w not aisici w a a isici Braided-only sought new obtained a. From the pulp of the carob pod, which is so much a by-product of the samence industry, they have aoral value and open new perspectives for seafaring. They thus contribute to the valuation of the product; maríoola rsgionsi ensuring a more competitive and sustainable agricultural future <. The use of dc; ultrasound method by ultrasound of the vrítò constitutes. Effective strategy for the selective attainment of inactive phenolic compounds, overcoming the problem of using conventional organic solutes. They are, like methanol, contributing to the economical shaping of the biomass in a non-solid bait from the biorrefinery. DBS0RXTION 00 METHOD ~ PrimaMa and its preparation An ma them having p rhyme are the residues of carob pulp (e.g., flea triturated locust ^Ç'kibblss? _F ^t 1J byproduct of carob processing industry 1 'use of. Its processing or seed waste induscriai , also called adaarroba bicccasea. Obtaining the extract · Xiquido consisting of these compounds. Phenomena A ma i è ria - gri ma · ^v k 1 bb 1 ss - go dg adsa 1 farro ta rri t. In addition, the waste is added with water or water. vau The lace on the front or shoulders or shoulders, in proportion to the meaning of the racquet, which is 1: 1. at 1:50 ° C (ra / v ?,) the bath is placed after a further temperature at the temperature rolled over a certain period of time. to be used in subsequent applications, the extraction process does not require that the dry and dry mow be used and can be used humidity, which corresponds to an industrially significant precaution. The lyophilized extract is characterized by the fact that it has in its composition specific phonolous compounds, and these compounds are identified as wild-type, e.g., gallocharged in the lighthouse, nourishes others. Evaluation lloflilitada of toer was Francoic extract tetraie The evaluation d the content extra otoal compounds ilofilinads is determined ruin wool. thistle. the reagent: ds bnli.n ·· _{fdoaplm.qap:.} in board adaptation d the bad whole: described by ni.nqlston er a.i., 1cJ, d tso r in total phenomena is expressed nm milligram equivalents of gallium acid (GAE) gram of lyophilic extract (mg PAE.fgl or g of equivalent equivalents is 1G10 glycolic acid per kilogram of crushed locust bean pulp. ' ^: xlodlos® (g GAn / Kop, as a function of a wave curve prepared for the affect using gel ice awning as a standard. Evaluation gives addictive activity of leached extract eratxate A a ++ a. +. The bark of the -ar + t ioxldaate activity of the lyof11.azate is incubated on the basis of the DPrH method described by Erand-Állllams et '1' + 26] which nunsiate in the capture of the DfPH radical (1,2- + 111 + 80.1 ll-picri by antioxidant, yields a decrease in the absorbance at 515 nm The reaction between the extract and the radical takes place for 30 minutes when the rasultad + a is expressed as an inhibition technique (radical wool ++). 2E ++ at the end of this time Antioxidant activity was also expressed relative to a standard, Trolox, {+++ m of the most patent antluxiducts, analogous to vitamin Et, to obtain the equivalent result of iroiox ( TEAc - Tr + ulox Equlvalent Ar + t iexidant Sapa + ti tyj, Evaluation of the antiproliferative activity of lyophiladate extxateThe evaluation of antip.roi 1 ferarlva activity. A.D? Extraction lyophilizate is determined by carcinogenic cell sanding::: Ineupa-dae +8+ -17 dl -in Sahara arm + ida ocm 61 of -®-, The ability of the +, et + + products obtained from pure crushed locust bean pulp to inhibit the growth of ugly + small cells is evaluated at +: + 1 + 11-1 later the best + is from MIA { o-+1, 1-dimethylthlacol-z-yl-2,5-di-diphenyl-tetrazolyl bromide; according to method 1 or 1a described by Mos s ma r + n + 2 + 1]. Survival of cells was expressed as percussion + tag: compared to untreated cells + to standard. OAMdO X The raw (10 g Carob pulp crushed d; d.dd ^':) is the ADI tamed 5'0 AD ud.ocáda d Agra and a barb of the AONs Uitra-Ambienta temperature for 30 d, uutds .. 1. (dime end of the éutracto liquid obtained is filtered through paper filter Watbman of u 1 and lidlldado. The lyophilized extract obtained from water in liquid, for the phenolic content and evaluation of oxidard, according to the methodologies extract presented a content of fendiimos mg GAu / á of bag extract (1, Õ2 q GAt / Kq of pulp). carob) to odor, from the radical. dd from ^: d, 37 mH Tdd / Kç pulping the robe d