RU2739254C2 - Method for initiation of tumor cell death by succinic and 3-aminophthalic acids and hf- and uhf-energy wave radiation - Google Patents

Abstract

FIELD: medicine.SUBSTANCE: invention is intended for inducing death of tumor cells in living biological objects of succinic acid (SA) and 3-aminophthalic acid hydrazide (APA) of preparation "Galavit" and energy of HF- and UHF-radiation, known as HF- and UHF-hyperthermia. Described is a method for initiation of tumor cell death by HF- and UHF-energy, intended for local treatment of oncological patients having tumor tissues, for their hyperthermia in all organs of human body, characterized by the fact that before treatment, person for 3 days is transferred to a protein diet for maximum multiple selective increase of accumulation in "hungry" tumor tissues high-electroconductive electron-ion solution of 3-aminophthalic acid hydrazide when intravenously administered in megadose 36 mg/kg of human body weight for 8 hours and oral administration of succinic acid in disposable megadose 3000 mg 2.5 hours prior to physiotherapy beginning with maximum accumulation of "Galavit" preparation in tumor tissues during this period, is 3–6 times higher than in healthy tissues, selective hyperthermia of tumorous tissues HF- and UHF-energy is performed in accordance with depth of their location and penetration depth of electromagnetic wave into human body by 6.1 centimeters, with allowed frequency f=2450 MHz, with total heating rate of tumor tissues at this frequency 0.078 °C/s, for 191 s to heating temperature of tumor tissues 51.5 °C, when heating healthy tissues no more than 40 °C.EFFECT: method leads to thermal death of tumor tissues due to high dielectric contrast of the tumor and increase in difference of its conductivity (dielectric losses) relative to surrounding healthy biological tissues, reaching multiple values for various tumors; this allows for fluorescence diagnostics to specify boundaries of tumors and to conduct simultaneous hyperthermia of tumor cells with electromagnetic HF- and UHF-energy of photo-wave radiation and detect and destruct, therefore, even undetectable tumor formations located in deep layers of biological object.1 cl, 2 tbl

Description

The invention relates to medicine and is intended to induce the death of tumor cells in living biological objects of succinic acid (UC) and 3-aminophthalic acid hydroside (ROS) of the Galavit drug and the energy of wave HF and microwave radiation, known as HF and microwave hyperthermia.

In medicine, hyperthermia is called a significant increase in human body temperature over 40 ° C. Hyperthermia for cancer treatment has been used as early as half a century ago. The German physician von Ardenne opened a "thermal" clinic in a water bath for hopelessly oncological patients, whom he heated to 42 ° C. After such a procedure, no more than 17% of people survived, but they were completely cured. The rest died, unable to withstand such high temperatures. This technology is still used in the United States, where the human body is heated to 42.5 ° C, with its subsequent return to life. This treatment technology can be effectively used for selective heating of oncological tissues with HF and microwave energy without a significant increase in the temperature of healthy tissues surrounding the tumor.

The method of initiating the death of tumor cells electromagnetic, consists in the complex simultaneous action of succinic and hydroside 3-aminophthalic acids and the energy of wave HF and microwave radiation, which include the tablets of the preparations "Succinic acid" and "Galavit" - Luminol, intended for intravenous administration and ingestion by a person, and wave irradiation with electromagnetic energy HF and microwave. The preparation "Galavit" - Luminol is a crystal-like powder of white-yellow hue. It dissolves very well in hydrogen peroxide and other oxidizing agents and organic liquids. Luminol does not dissolve in water.

After taking a solution of succinic and 3-aminophthalic acid hydroside and for their preservation in a significant amount in the tumor tissues of the human body, time is required for their accumulation in tumor tissues with 3-aminophthalic acid hydroside for 6-8 hours and the maximum content of succinic acid is 0.5 -2.5 hours. The maximum saturation of 3-aminophthalic acid hydroside in tumor tissues occurs precisely during this period of time and it is 3-6 times higher in tumor tissues than in healthy ones due to the selective absorption of acid by tumor tissues.

A number of researchers argue that succinic acid, which is a prooxidant that neutralizes free radicals, in the blood and tissues kills cancer cells without affecting healthy ones due to the local oxidative stress caused by the process of damage caused by oxidation of cellular DNA and depletion of adenosine triphosphate (ATP) - energy source of the cell. Succinic acid, among other accompanying molecules, due to aggressive action, causes a malfunction of the functioning of a certain enzyme responsible for the "nutrition" of cells of malignant tumors. YaK is a representative of intermediate acids that can accumulate in the cytosol of cells.

Tumor cells accumulate, in contrast to normal cells, a significant amount of succinic acid.

Scientists have proven that succinic acid and succinates are adaptogens (increase the body's resistance to adverse environmental factors). Succinic acid stimulates the process of oxygen supply to cells, relieves stress, restores energy metabolism, normalizes the process of producing new cells, and has general strengthening and regenerating properties. The activity of succinic acid in the human body is regulated by the hypothalamus and adrenal glands.

Succinic acid has no side effects and is practically harmless. Its only unpleasant quality is that it can irritate the gastric mucosa, so it should not be consumed on an empty stomach. Also succinic acid has the ability to enhance the therapeutic effect of medicines. Succinic acid acts only where there is a shortage of it, accumulating in damaged areas, that is, its effect is targeted, which is practically impossible to say about any drug.

Succinic acid serves as a universal intermediate metabolic product released during the interaction of saccharides, proteins and fats in living cells. The activity of succinates in the body is associated with the production of energy spent on the vital activity of all organs and systems. With an increase in the load on any organ or system of the body, the energy for their work is mainly provided as a result of the oxidation of succinates. The succinate energy production mechanism works hundreds of times more efficiently than all other energy production mechanisms in the body. Thanks to this, succinic acid has a nonspecific therapeutic effect in a number of diseases of different etiology. Also succinic acid has antiviral and antihypoxic effect.

Laboratory studies have shown that the use of succinic acid caused a more intensive absorption of oxygen by living cells. The oxidation of succinic acid is a necessary step in the process of assimilation of diatomic oxygen by cells. The therapeutic effect of succinates is based on a modifying effect on cellular metabolism - cellular respiration, transport of trace elements, and protein production. In this case, the degree and specificity of modifications depend on the initial state of the tissues. As a result of such modifications, the parameters of tissue work are optimized.

Restoring the balance of biochemical reactions in the body, succinates normalize the functions of all organs and tissues. Their effect on the brain is especially significant, which most of all needs an uninterrupted supply of oxygen and energy. Therefore, succinic acid is used for the prevention of brain pathologies that develop during aging. In addition, it restores the functions of the entire nervous system and prevents stress.

Additional consumption of succinic acid contributes to the normalization of the work of other organs and systems.

In situations where oxygen delivery is insufficient to provide a functional load (work) with energy, anaerobic glycolysis is activated, and the end product of glycolysis, lactic acid (lactate), accumulates in the tissues. All living cells - whether they are animal or plant cells, fungi or bacteria - contain special little bodies a few microns in size, which are called mitochondria. In mitochondria, succinic acid is mainly formed and used for subsequent reactions.

With sufficient oxygen supply, all organic acids are burned in specialized cell organelles, mitochondria, due to oxygen consumed from the air.

Under aerobic conditions, the products of glycolysis, fatty acid oxidation and amino acids are burned-oxidized with the participation of oxygen in mitochondria - cellular organelles that play the role of the main source of energy. Accumulation occurs in the respiratory chain, a polyenzyme complex located in the mitochondrial membrane of negatively charged riticulums.

As the research of Professor of the Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences M.N. Kondrashova, the energetic power of the ATP synthesis process during the oxidation of succinic acid is significantly higher than during the oxidation of any other substrate. That is why many energy-dependent, that is, energy-consuming processes, for example, the accumulation of calcium ions and the provision of biosyntheses with hydrogen, even in isolated mitochondria, can proceed only during the oxidation of succinic acid. The works of the school of M.N. Kondrashova showed that in nature there exist and, if necessary, are activated additional pathways for the formation of succinic acid. In particular, such an additional "injection" of succinic acid in a healthy person occurs during intensive work and during the recovery period after exertion, when the need for rapid ATP reproduction is especially high.

During hypoxia, the respiratory chain of mitochondria cannot accept hydrogen from any other substrate, except for succinic acid. After all, it is during its oxidation that hydrogen enters the part of the respiratory chain much closer to oxygen. At the same time, even with deep hypoxia, the ability to accept hydrogen remains on the site. In this case, the oxidation of succinic acid in mitochondria remains one of the few sources of ATP. Additional intake of succinic acid can significantly help the body's vital functions.

The succinic acid that is formed in the mitochondria instantly burns out in the same place, therefore the current - stationary concentration of the succinic acid present in the tissues does not exceed 10-20 mg / kg of tissue mass at each moment of time and, as a rule, does not leave the mitochondria. Outside the mitochondria, outside the cell, it is practically absent in the bloodstream. It appears outside the mitochondria during severe anaerobiosis (complete absence of oxygen) or during deep hypoxia in some area of tissue.

Receptor control systems of the body evaluate the appearance of succinic acid in the bloodstream as a signal that in some part of the body there are not enough energy resources, or there is oxygen starvation. Respectively. The body reacts to this signal with shifts in neuroendocrine hormonal regulation, improved peripheral blood flow, increased heart rate, facilitated oxygen release by oxyhemoglobin, and a number of other physiological and biochemical compensatory reactions.

In conditions of a lack of oxygen, some of the cells die, while other cells adapt to such conditions and change. They make up for the lack of energy not due to the supply of oxygen, but due to the development of their internal activity. The breathing disorder that leads to cancer is not severe enough to lead to cell death.

Formation of cancer cells from normal cells occurs in two stages. First, after an irreversible breathing disorder, a rather long period of an imperceptible course of the disease occurs. The affected cells seem to defend their right to exist. It takes at least 2 years from the moment the first cancer cell is formed to the formation of a clinically detectable cancer. Energy reactions are much more active in the affected cells. Hence the possibility of treating cancer by influencing the energetic reactions in the cells. Succinic acid is a unique agent that can influence energy metabolism.

Known complex for the diagnosis and destruction of tumor tissues with lasers and hydroside 3-aminophthalic acid converting into aminophthalhydroside. This complex with 3-aminophthalic acid hydroside or its derivative aminophthalhydroside penetrates the cell along a pathway involving receptors with 3-aminophthalic acid hydroside on the cell membrane, due to polysaccharides taken by onco cells for glucose. This complex with 3-aminophthalic acid hydroside or its derivative is used to obtain drugs with antitumor activity.

Hydroside of 3-aminophthalic acid is oxidized by hydrogen peroxide of a derivative of succinic acid in an alkaline medium, the reaction is catalyzed by manganese, and causes chemilumination, the strongest that was observed in the near future. If an oxidizing agent, hydrogen peroxide, is added to an alkaline solution of this hydroside, then a bright blue glow flares up. In the presence of catalysts, this glow intensifies and becomes very bright, which can be used as a source of illumination. The role of catalysts for a solution of 3-aminophthalic acid hydroside is carried out by blood iron hemin, various compounds of potassium, copper, etc. These chemical activators of chemiluminescence enter into chemical reactions with reactive oxygen species or organic free radicals, during which cell molecules are formed in an excited electronic state. The observation with this glow is associated with the transition of molecules to their ground state, which leads to the emission of photons. The excited state activator is 3-aminophthalic acid luminalhydroside in the presence of oxygen radicals. Under the action of an oxidizing agent - a hydroxyl radical, a luminol radical is formed, which reacts with a superoxide radical, which forms an internal peroxide (dioxide), which lead to the formation of an excited 3-aminophthalate molecule. The transition of this molecule to the initial ground state is accompanied by emission of a quantum of light. Hydrogen peroxide is the main participant in the formation of free radicals, it is constantly formed in small quantities in the human body, this is a relatively harmless compound, but in the presence of metal ions of variable valence iron, copper, manganese and chromium or hemin compounds from hydrogen peroxide Н ₂ О ₂ , a destructive hydroxyl radical is formed JOH, which causes mutations and inactivation of enzymes and damage to biological membranes of cancer cells. The hydroxyl radical of luminal actively enters into a chemical reaction with it, which leads to a bright glow of biological tumor tissues.

Some cells of the body: granulocytes and monocytes in the blood, and tissue macrophages, in the fight against foreign cells, release reactive oxygen species contained in superoxide radicals, hydrogen peroxide H ₂ O ₂ , and the hydroxyl radical JOH in this case, weak chemilumination is observed, which increases in a thousand times in the presence of 3-aminophthalic acid luminal hydroside. These effects are multiplied by the action of short-term electrical impulses on blood vessels and cells, causing an increase in the permeability of cell membranes - riticula and stimulation of the release of reactive oxygen species by the mitochondria of cells, which leads to a bright blue glow of biological tumor tissues.

When placed in a variable electromagnetic field of high intensity and frequency of various biological bodies, they also begin to emit a characteristic radiance of varying intensity and colors, by which one can judge the properties of the object under study. The method of "high-frequency photography" (the Kirlian effect, Kirlianography in honor of the inventor V.Kh. Kirlian) is now widely known in Russia and abroad as a method of experimental research of electromagnetic fields and bioenergetic interactions. But of the greatest scientific and practical interest are studies of the glow of biological objects in an alternating electromagnetic field of high frequency, explained by the photoelectromagnetic effect of photowave radiation and luminescence of biological objects.

In accordance with modern concepts, aqueous solutions of alkalis and acids in the human body are considered as an associated liquid, consisting of separate associated elements - neutral clusters and cluster ions of the general formula (Н ₂ O) _n , [(Н ₂ O) _n ] ⁺ , [( H ₂ O) _n ] ^- , [(NO ₂ ) _n ]. [(H ₂ O ₂ ) _n ], [(NaO ₂ ) _n ] [(ClO ₂ ) _n ], [(CO ₂ ) _n ], etc., where the number of hydrogen-bonded water molecules can be n times to reach, according to some authors, under the influence of HF and microwave energy hundreds and even thousands of units. These effects respectively alter the electrical conductivity and biophotoluminescence of biological tissues. A change in the position of one structural element (water molecule) under the influence of any external factor or a change in the orientation of the surrounding neighboring water molecules in the cells provides high sensitivity of the entire information system of water to various external influences (electromagnetic, thermal, sound fields, bioinfluence, etc.). In addition, in water clusters, due to the interaction between covalent and hydrogen bonds between oxygen atoms and hydrogen atoms, the proton (H ⁺ ) can migrate according to the relay mechanism, leading to proton delocalization within the cluster, providing the release of singlet oxygen with a characteristic bright glow that kills cancer cells. This property explains the extremely labile, mobile nature of the interaction of clusters with each other.

The structured state of aqueous solutions is a sensitive sensor of various fields - electromagnetic, acoustic, energy-information, etc. In addition, aqueous solutions of various chemical elements are a source of superweak and weak alternating electromagnetic radiation. In this case, the induction of an external electromagnetic field can occur, causing resonant effects of combination (superposition) of external electromagnetic fields with their own fields in biological objects with photowave radiation, capable of changing the structural and informational characteristics of biological objects, 80-90% consisting of water solutions with various chemical impurities and cause their photoluminescence.

Under the influence of a high-frequency electromagnetic field in biological objects and aqueous solutions of various chemical substances, excitation, polarization and ionization of N ₂ , H ₂ , O ₂ and CO ₂ molecules occur. As a result, an ionized gas is formed with separated electrons having negative charges, which create an electrically conductive medium for the formation of a corona discharge in biological objects of various colors, which, depending on the electrically conductive properties of an object saturated with various chemical solutions, can color the glow crown in different colors. The shape of the glow crown, its density, brightness and surface distribution are determined mainly by the electromagnetic parameters of the object.

Some cells of the body, granulocytes and monocytes in the blood, and tissue macrophages, in the fight against foreign cells, release reactive forms of singlet oxygen contained in superoxide radicals, hydrogen peroxide H ₂ O ₂ , and the hydroxyl radical JOH in this case, weak chemilumination is observed, which increases many times in this case, a weak chemilumination is observed, which increases many times in the presence of succinic acid and Galavit preparations, with HF and microwave irradiation. These effects are also greatly enhanced by the action of short-term electrical impulses on blood vessels and cells, causing an increase in the permeability of cell membranes - riticulomas and stimulation of the release of reactive oxygen species by the metachondria.

This effect of exposure to electrical impulses at the beginning of the 19th century was successfully demonstrated to the public by Nikolo Tesla, when he irradiated pulsed high-frequency energy from an open antenna of an RF generator located in the room of irradiated vessels with liquids capable of emitting light and fluorescent lamps, which, without being connected to electrical wires, shone bright light in the hands of Nikola Tesla, which he also juggled with. This caused genuine delight among the audience, while an inexplicable phenomenon in nature.

These factors in biology are called collective stimuli in the presence of luminols that change the state of blood and tissue phagocytes and their ability to increase the release of reactive oxygen species, and, accordingly, the protective functions of cells.

If the treatment was based on powerful doses of UC against the background of complete overlap of the intake of carbohydrates - glucose and further exposure to HF and microwave energy of cancer cells, the effect of electromagnetic photoluminescence and their damage would be even higher. For this, in our opinion, it is necessary to transfer a person to a carbohydrate-free diet for 3 days, for a complete absence at this time in the human diet of carbohydrates, which in the gastrointestinal tract are converted into glucose, which is extremely necessary for the nutrition of cancer cells. With this introduction of cancer cells into artificial glucose "starvation", then a person needs to take high doses of the preparation of succinic acid and "Galavit", their oxidized form succinates and hydrazide of 3-aminophthalic acid. When they enter the blood vessels of the tumor, the blood thickens with a large branched network with thin peripheral vessels and a low speed of blood flow in them when heated, decreases even more, which leads to blood coagulation in the vessels of tumor tissues, not allowing them to cool, due to the absence of a closed circulatory system. In the main human organs, rich in venous vessels, closed in the main circulatory system, there is a cooling of borderline healthy tissues exposed to HF and UHF hyperthermia. A "hungry" tumor is maximally saturated with succinic and hydrazide 3-aminophthalic acids, several times higher than in normal healthy tissues, stimulates the formation of hydrogen peroxide, in a sufficiently large amount in the interstitial and intercellular fluid. It is this chemical compound that is formed in the process of interaction between the YAK drug and "Galavit" in the internal organs of the body. Hydrogen peroxide and lipoperoxide are factors that stimulate the mechanisms of self-destruction and death of cancer cells due to the large release of active oxygen. The formation of sufficient doses of hydrogen peroxide and other lipoperoxides around and inside the cancer cells and their apoptosis is possible only with a sufficiently large intake of succinic and 3-aminophthalic acid hydrazide. Under these conditions, YaK and ROS can act as an antioxidant or prooxidant, i.e. an oxidizing agent, including showing a destructive rather than a creative property of cancer cells. This is very important in the energy of cells. Therefore, UC and ROS can be designated as a switch of metabolism, which accelerates and optimizes aerobic energy metabolism in normal cells, stimulates tissue respiration and the formation of ATP. In cancer cells, aerobic respiration is absent in mitochondria and is replaced by glycolysis. UC and ROS, upon entering the oncocell, inhibit glycolysis, but are unable to transfer it to the path of normal aerobic activity. Perhaps this is due to the competitive presence of glucose. For the complete shutdown of glycolysis in tumor cells, it is necessary to completely exclude the access of glucose or so that UC and ROS prevail over glucose in the substrate. In healthy cells in small amounts in cytazole, it exhibits protective antioxidant properties. In cancer cells, with an excess of it, it stimulates the oxidation processes, with the formation of hydrogen peroxide and lipid peroxides, which, when they are in excess, have a toxic effect on onco cells.

Succinic and 3-aminophthalic acid hydrazide are actively imported into the endoplasmic reticulum (EPR) (Endoplasmic reticulum, consisting of membranes and specifying the direction and active transport of substrates against gradients) of cells with the help of glucose transporters. It should be noted that energy processes in cancer cells are transferred from mitochondria to the endoplasmic reticulum. It is here in the EPR that UC and ROS accumulate, and the environment of the oncocell in this place differs significantly from ordinary cells. Hydrazide of 3-aminophthalic acid is simply re-reduced here, and here YaC is obviously forced to be reduced to succinates. From this moment on, the destructive effect of UC and ROS on the oncocell begins. A "hungry" cancer cell at this time can repeatedly accumulate UC and ROS in itself, because perceives them on its membrane transporters for glucose. Since there are many times more glucose-consuming receptors in the oncolet than in healthy ones, although the transport systems for the delivery of glucose and UC and ROS into cells are common, this is a "Trojan horse" for oncocells. Thus, it is very easy to deceive the oncocells and inject UC and ROS into them, with the solution of the problem of supplying megadoses of UC and ROS, and then the phenomenon of cancer cell death will be greatly enhanced

A "hungry" tumor is maximally saturated with 3-aminophthalic and succinic acid hydrazide, 3-6 times higher than in normal healthy tissues, stimulates the formation of macrophages and T-lymphocytes under the action of the ferrachelatase enzyme, in a sufficiently large amount on membranes and interstitial fluid. It is this chemical compound that is formed during the interaction of 3-aminophthalic acid hydrazide, succinic acid and the internal environment of the body. Under the action of the oxidizing agent hydraxyl radical and hydrogen peroxide, significantly enhanced temperature action and additional action, electromagnetic fields of HF and microwave, the luminal radical is formed, which then reacts with the superoxide radical, forming internal peroxide (dioxide), and hydrogen peroxide Н ₂ О ₂ with high-frequency and microwave-hyperthermic decomposition, vitamin B ₉ UC, there is a multiple increase in the formation of excited 3-aminophthalate molecules. The transition of this molecule to the ground state is accompanied by the emission of light quanta, and a strong blue glow, as a result of chemical reactions associated with a high release of reactive oxygen species and organic free radicals that burn out cancer cells.

The method of "selective starvation" of superficial and deeply located onco cells in the human body, through the subsequent introduction or administration of various sensositizers, for selective maximum saturation of tumor cells with highly electrically conductive electron-ion solutions of sensitizers with a maximum separation of the electrophysical properties of tumor and healthy tissues, followed by a selective effect on them electromagnetic fields of high frequency in combination with other methods - this is the most relevant scientific and practical direction in the fight against cancer, which has a stable toxic effect on cancer cells.

The study of biophysical and biochemical mechanisms determine three concepts of cancer cell death, one assumes the significance of D-YaK and UC, the other of 3-aminophthalic acid hydrazide, and the third HF and MHF hyperthermia, which in these three cases leads to the apparent death of cancer cells.

A number of researchers argue that the minimolar concentration of YaK and ROS, which are prooxidants (lyco-oxidizing compounds that neutralize free radicals), in the blood and tissues kill cancer cells without affecting healthy ones, due to the damage caused by local oxidative stress, as a result of oxidation, of cellular DNA and depletion of adenosine triphosphate (ATP), the cell's energy source. Hydrogen peroxide and other lipid peroxides, along with other accompanying molecules, of aggressive action, cause a malfunction of the functioning of a certain enzyme responsible for the "nutrition" of cells of malignant tumors. YaK and ROS are representatives of intermediate acids that can accumulate in the cytosol of tumor cells and, presumably, under the enhanced action of photoelectromagnetic HF and UHF energy, destroy them.

Tumor cells accumulate, in contrast to normal cells, a significant amount of homocysteine teolactone (HTL). Before homocysteine is inserted into a protein, it becomes a biological marriage, in the form (HTL). In ordinary cells, there is little homocysteine, therefore, theolactone is practically not formed from it, but transformation into a cancer cell requires a significant activation of methylation, which in turn starts a special biochemical cycle in which homocysteine is involved. In this case, the protein that synthesizes the machine of the cancer cell works at full capacity, so it is more often mistaken. Teway found that interaction with organic acids, this substance forms the highly toxic 3-mercaptopropionaldehyde (MPA). When succinic acid and 3-aminophthalic acid hydrazide enter a cancer cell saturated with HTL, MPA is formed, which kills the cancer cells. Destroying cancer cells, MPA eliminates the source of its formation, so normal cells do not suffer from it much under the influence of 3-aminophthalic acid hydrazide.

During high-frequency and microwave heating, succinic and 3-aminophthalic acid hydrazide in onco cells is converted into a solution of ionic salts of succinates, and aminophthalhydroside under the action of temperature, with the formation of hydrogen peroxide and other lipoperoxides. The more succinic acid and aminophthalhydroside in the tumor cell, the more lipoperoxide and hydrogen peroxide are formed in it, in comparison with healthy cells. An excess of hydrogen peroxide and lipid peroxide triggers the photoelectromagnetic hyperthermal and photoluminescent mechanism of cancer cell death. The process of cancer cell death is initiated by HF and UHF fields by rapid heating and electromagnetic photoluminescence of cancer cells by heating them to 55.5 ° C saturated with YaK and ROS, their rapid oxidative decomposition under the influence of temperature with a high release of hydrogen peroxide and lipid peroxides causing photoluminescence with high release of active oxygen, which is destructive for cancer cells.

In healthy cells, UC and ROS, entering the ER, will not be reduced to a solution of ionic salts of ROS succinates, because pH and ORP (oxidation-reduction potential) are not suitable for this, and YaK and ROS will be practically harmless for them and will be transformed on the glucose conveyor. In oncocells, the environment is different, re-reduced by succinic (D-YaK) and 3-aminophthalic acid hydrazide succinate ions, which try to burn and destroy everything in the oncocell to the maximum, due to lipid peroxidation (LPO). In this case, significant destruction occurs with the formation of toxic lipoperoxides that damage cell membranes, various organelles, mutation of nucleic acids, enzyme inoculation, destruction of nutrients and cell death. In this case, cell death does not follow the path of apoptosis, but a powerful necrosis.

In this case, it can be argued that in the treatment of cancer of succinic and 3-aminophthalic acid hydrazide, obtained as a result of the oxidation of YaK and ROS, both in the blood and in various human organs, a therapeutic effect is observed. This cure effect is explained by the fact that in this time in normal cells of living biological objects of succinic from 0.5 to 2.5 hours and hydrazide of 3-aminophthalic acid from 6 to 8 hours is quickly converted into ionic salts of succinates and aminophthalhydrazide and ferrous heme of iron, under the action of the enzyme ferrochelatase, while maintaining a high the contrast of the maximum content of succinic acid and 3-aminophthalic acid hydrazide in tumors is 3-6 times higher than in healthy ones and their further transformation into succinic and aminophthalhydroside in tumors, which significantly increases their electrical conductivity with a significant increase in the dielectric properties of tumors relative to the surrounding healthy biological tissues, reaching this difference several times. With further electromagnetic HF and microwave hyperthermia and photoluminescence of tumor cells saturated with succinic and 3-aminophthalic acid hydrazide, for 191 s at a heating rate of 0.078 ° C / s to the final heating temperature of tumor cells of 51.5 ° C with photowave radiation of HF and microwave fields, they are destroyed. Heating and hyperthermia of tumor tissues saturated with a solution of 3-aminophthalic acid hydrazide of the Galavit preparation and YAK is produced by HF and microwave energy of wave radiation, in accordance with the frequency, with the depth of penetration of the electromagnetic wave and with the depth of the location of tumor tissues, at allowed frequencies f = 13.56 MHz-1100 cm, f = 27 MHz-545 cm, f = 40.68 MHz-370 cm, f = 433.92 MHz-34.5 cm, f = 915 MHz-16.5 cm and f = 2450 MHz - 6.1 cm.

The most complete accumulation of succinic acid in the tumor occurs within 0.5-2.5 hours after taking a single dose of 2400 mg and 3-aminophthalic acid hydrazide occurs within 6-8 hours after taking them orally in a single dose of 100 mg. Then the level of succinic and 3-aminophthalic acid hydrazide in the tumor, respectively, gradually decreases, reaching the initial values, respectively, 2.5 and 24 hours after taking the drugs.

In the proposed method, YAK and Galavit are taken orally at the rate of a megadoses of 36 mg / kg of a person's live weight 8 hours before treatment with HF and UHF energy and succinic acid with a megadoses of 3000 mg orally 2.5 hours before HF and Microwave hyperthermia of tumor tissues with the energy of photowave radiation, after which, at a heating rate of 0.078 ° C / s for 191 s, to a temperature of 51.5 ° C, as a result of which the tumors are denatured and, as a result, the decay products of tumor cells are excreted by the body during 2-4 weeks independently, in a natural way, excluding surgical intervention in the human body.

The main task for researchers is to maximize the effect of maximum selective absorption of ROS and UC by cancer cells with simultaneous subsequent high-frequency irradiation of cancer cells in order to increase the effectiveness of treatment up to 100%. It has already been proven that such an effect is possible, and most importantly, that it is harmless, without any special side effects.

Numerous studies carried out by us at the Krasnoyarsk State Agrarian University and VIZR in St. Petersburg have confirmed the 100% efficiency of disinfection of vegetable seeds and living biological objects saturated with highly conductive electron-ionic solutions of microelements with high-frequency and microwave-energy against viral infections that have a similar origin with cancer cells.

A.S. No. 563938 USSR. Method of processing seeds of agricultural crops / Tsuglenok N.V., Tsuglenok G.I. - Publ. 03/16/1977, Bul. No. 25. Certificate of the USSR No. 950214. Method of pre-sowing seed treatment / Tsuglenok N.V. - Registered in the registry on 04/14/1982. 45. Intensification of thermal processes of seed preparation for sowing with high-frequency and microwave energy: guidelines / N. V. Tsuglenok. - M .: Agropromizdat, 1989. Guidelines for the use of high-frequency and microwave energy in the preparation of seeds for sowing / N.V. Zuglenok. - M .: RZh Gosagroprom of the USSR, 1989 .-- 19 p. Ways of disinfection of tomato seeds against viral infection / Yu.I. Vlasov [et al.] // All-Russian Research Institute of Plant Protection (VIZR). - 1989. - T. 71. - S. 49-54. Method for disinfecting egg powder. Patent number: 1734632. Published: 23.05.1992 Authors: Tsuglenok N.V., Kolmakov Yu.V. IPC: A23v 5/02. Method of preparing a medium for diluting semen from the manufacturer. Patent number: 1769422. Published: 27.06.1995. Authors: Tsuglenok, Ostashko, Shakhmatov, Silantyeva, Kontsedal.

It has been proven that under the influence of carcinogens, oncoviruses are incorporated into a healthy cell and eventually dissolve in it, turning it into an oncocell. Any viruses are killed by temperature or acid. Other methods against oncoviruses and cancer cells are basically powerless, they simply are not. A new photodynamic method of using laser photosensitizers deserves special attention in this direction. But the shallow depth of penetration of the electromagnetic wave of laser emitters does not allow burning deep-seated malignant tumors.

It is necessary to note another very important biophysical process that occurs when biological objects are saturated with solutions of trace elements, this is an increase in the specific conductivity of viruses consisting of a protein envelope filled with a mixture of nucleic acids and, similarly, tumor tissues filled with solutions of intercellular fluid determined by a significant concentration of ions and electrons and their mobility in comparison with healthy tissues. With an increase in temperature during HF and microwave heating in tumor tissues, the mobility of ions and electrons increases significantly, increasing their electrical conductivity and dielectric losses, which further enhances their selective heating with HF and microwave energy and causes apoptosis of viruses and tumor tissues.

The most important thing is that this method is environmentally friendly and harmless, does not have any special side effects for biological objects.

This proposed technology allows simultaneous fluorescence diagnostics to clarify the border of tumors and simultaneous hyperthermia of tumor cells with the energy of wave radiation with the decomposition of UC and ROS into hydrogen peroxide and hydroxides allowing to effectively identify and destroy, thus, even undetectable tumor formations located in the deep layers of biological object.

The biophysical meaning of this method lies in the selective maximum saturation and accumulation of highly electrically conductive electron-ion solutions of electrophotosensitizers in tumor cells and in the maximum separation of the electrophysical properties of tumor and healthy tissues saturated with succinic and hydrazide 3-aminophthalic acid, and a significant increase in the difference in the electrical potentials of tumor cells and healthy cells. in the intercellular environment and on the walls of the reticulum. The reticulum is an electrical circuit, where negative charges are evidently accumulated on one side of the membrane, and positive ones on the opposite side, therefore the reticulum is an electric transporter of glucose and other nutrients of cancer and healthy cells. Consequently, the reticulum is an electrical network charged with negative and positive charges. The balance of these charges is strictly controlled by the activity of mitochondria and the energetic operator structures on the outside of the cell - on the cilia. These proteins, under certain situations in the environment of the cell, being discharged, can give an active signal to the reticulum and mitochondria. This changes the balance that exists on one side of the reticulum. This leads to a shift in chemical processes, many new reactions are triggered. One side of the reticulum membrane is connected to one type of entrance to the mitochondria, and the opposite side to the exit from it. Thus, a single electrical circuit is created for double active control of mitochondrial energy. The intensity of the electric field on the reticulum keeps mitochondria under control. In this case, the mitochondria draw in the charges accumulated on one side of the reticulum membrane, and remove the opposite charges to the other side of the reticulum membrane. The charges are thus not mixed and separated. This is important for ion exchange to take place in cells. Externally, the reticulum is similar to the capacitor plates, the more layers of plates, the greater its electrical capacity. Between the spacers there is a semiconductor saturated with succinic acid and 3-aminophthalic acid hydrazide. This capacitor, i.e. a powerful dense network of tumor sheaths-membranes is very clearly visible through a microscope. The number of membranes in tumor cells is much higher than in healthy ones. Accordingly, the density of tumor tissues and the capacity of the biological electrical capacitor are significantly higher than those of healthy tissues. When charging, negatively charged particles-electrons will be collected on one plate of such a capacitor, and ions, positively charged particles, will be collected on the other. Such a charged capacitor can turn into a current source if disconnected. Any fluctuations in the external field on the outer side of the cell membrane affects the state of the reticulums, which discharge charge on the mitochondria, controlling their activity. The mitochondria, in turn, are tuned so that they never allow the charges on the reticulums to drop below a critical level. In cancer cells, the charges inside the mitochondria are sharply reduced and the entire regulation system is disrupted. This is the main control rod for the entire electrochemical energy of the cell. Therefore, chemical processes are always secondary and not primary. As a result of the electrochemical energetics of the cell in the reticulums there is a circulation of substances, where the mitochondria are the pump. With a lack of this circulation between the reticulums and mitochondria, due to electroosmosis, substances are sucked from the outside through the outer membrane and the gateways and sodium pump are opened on it. The medium on the membranes of the reticulum and the alkaline liquid substrate in tumor cells is re-reduced due to the excess of negative charges. This is what determines the chemical equilibrium in terms of pH, conjugated buffer chemical electrochemical substances, when the buffer system is discharged or restored. These processes are regulated by charges on the lining of the reticulum and metachondria. Chemical processes, in this case, are simply executors, intermediaries. The outer side of the metachondria provides a voltage dependent anion channel. This voltage maintenance mechanism is called VDAC, which sets the conditions for the work of the reticulum. It is here on the outer side of the membrane that the Hexokinase II enzyme is located, which ensures the utilization of glucose. Maximum separation, disconnection of the outer mitochondrial membrane (VDAC) and Hexokinase II of tumor and healthy cells provides the induction of apoptosis of tumor cells.

The mitochondrion works by pulling from the reticulum into itself as an electromagnetic pump, which requires power under high voltage. Without this effect, the highest tension of pulling in nutrients into the cell will not be possible. This process of self-regulation of metabolism includes the so-called cilia and conformational proteins, which work as a single closed energy circuit. Cancer cells, unlike normal cells, do not have cilia. This most damaging energy level is absent in cancer cells. The only correct way is the maximum separation of properties, oncological and healthy tissues and finding a weak point in the energy of cancer cells and thereby destroying them. Mitochondria set the state of charge for ion pumps on the outer membrane of the cell and for starter structures that hold charges on the reticulum. These sensory structures can be most rapidly damaged and burned out, since metachodria are the most efficient electrochemical furnaces. In the case of turning off the metachondria, the voltage gradient of the cell decreases sharply and the processes take place in onco cells over a much larger area, which allows them to burn a lot of glucose and other substrates such as ketones. There is no high degree of glucose combustion here. The oncocell takes not quality, since everything is concentrated on a small area of metachondria, but there are more of them, much larger than in healthy cells and, accordingly, at high potentials on the capacitor plates, i.e. a large amount of oxidation-combustion area on the walls of the reticulum network. Therefore, such a cell does not need oxygen, but the consumption of glucose will be much greater than in healthy tissues.

The membranes of the reticulum and the nucleus of the cell are the same, and the reticulum, as a capacitor, is contoured to the nucleus by only one of its side, an electron, and dumps electrons into the nucleus. Thus, the charge in the reticulum also provides charge inside the cell nucleus. The cell nucleus is saturated with electrophilic proteins, which provide the concentration of a super-powerful electrostatic charge inside the nucleus.

The main task for researchers remains to enhance as much as possible the effect of the selective maximum absorption of succinic and hydrazide 3-aminophthalic acid by cancer cells and to increase the effectiveness of treatment by increasing the electrical conductivity of the metachondria and reticulums of cancer cells. It has already been proven that such an effect is possible, and most importantly, that it is harmless, without any special side effects. The electrical conductivity of cancer cells is due to the presence of mobile charged electrons in the reticulums and in the cell nucleus and ions in the mitachondria of the cell. The amount of electrical conductivity depends on the amount of electrical charges and their mobility. The electrical conductivity of living tissues is determined by the concentration of ions and their mobility, which is different in different tissues, and therefore, biological objects have the properties of conductors, semiconductors and dielectrics.

The intercellular fluid contains the maximum content of ions and the specific electrical conductivity of tumor tissues is high and amounts to more than 1 S⋅m-1 without saturation with acids. Large protein molecules have a lower electrical conductivity, up to 0.003 S⋅m-1. Intracellular membranes have a conductivity below (1-3⋅10 ^-5 ) S⋅m-1. The highest values of electrical conductivity in the human body are found in liquid media (blood, lymph, bile, urine, cerebrospinal fluid and tumor cells 0.6-2.0 Sm-1) and muscle tissue (0.2 Sm-1 ). Bone, adipose and nervous tissues have the lowest electrical conductivity, especially coarse-fibrous connective tissues and tissues of tooth enamel (10 ^-3 -10 ^-6 S⋅m-1). The electrical conductivity of cells and tissues at HF and microwave currents is much more complex.

In this case, biological objects have both conductivity and capacitance, which characterizes the dielectric constant. The frequency dependence of the electrical parameters and the absorption of the energy of the electromagnetic field are determined by the size and shape of cells, the magnitude of their permeability, the ratio between the volume of cells and intercellular spaces, the concentration of free ions in cells and the content of free water in them. All these factors lead to a change in the electrical conductivity of biological objects. A particularly significant factor for the metabolism of cancer cells is the content of glucose or its substitutes, in this case succinic and 3-aminophthalic acid hydrazide. If the human body has malignant tumors and stage 3 and 4 metastases, which actively and intensively assimilate glucose or its substitute - succinic and 3-aminophthalic acid hydrazide, they are converted to ATP in cancer cells much less than in healthy ones, as a result of which, cancer cells get very hot and raise the temperature of the human body by 1-2 ° C. This physiological mechanism induces an increase in the temperature of tumor and surrounding normal tissues. The total rise in temperature in the human body is currently recorded by a microwave radiometer, which makes it possible to control the temperature of various organs deeply located in the human body with an accuracy of 0.3 ° C.

This process was partially studied by us when exposed to a biological object with tumor tissues, which were subjected to daily complex action of a constant magnetic field with an intensity of 25 μT and an alternating magnetic field with a frequency of 3.1 Hz and an intensity of 5 μT, exposure for 60 minutes a day at a time, for 5 days. The proposed method of influencing constant and variable effects on ion exchange in mitochondria of cells and on negatively charged electrons on reticulums and cell nuclei made it possible to induce death of tumor cells using magnetotherapy, which by 40%, compared with control, freed biological objects from tumor cells ( Patent No. 2307681, authors: Tsuglenok N.V., Sergeeva E.Yu., Klimatskaya L.G. RU). Therefore, this direction of using magnetic and electromagnetic fields and their effect on the energy of tumor cells deserve special attention, confirmed by researchers from South Korea, who suggested using a powerful magnetic field to destroy tumor cells. In a powerful magnetic field, the tumor begins to kill itself.

There is a known method of destruction of cancer cells with microwave irradiation (RF Patent No. 2174021, IPC A61N 5/02) before exposure to hyperthermia, the tumor is exposed to microwave radiation with a wavelength of 1.3-2 cm and the value of the resonant frequency is absorbed by the tumors. After that, a similar effect is carried out on healthy tissues bordering with the tumor, and the value of the resonance frequency of the absorption of these healthy tissues is determined. Simultaneously with hyperthermia, the values of resonance frequencies of energy absorption by tumors and healthy tissues are monitored, and when the values of resonance frequencies approach, energy absorption by tumors and healthy tissues is judged on the effectiveness of treatment. This method makes it possible to increase the efficiency of tumor treatment by the method of microwave hypothermia when they are heated to 43 ° C.

The main disadvantage of this method is the slight difference in heating of tumor and healthy tissues.

There is a known method for the destruction of cancer cells of tumor tissues (RF Patent No. 2106159 IPC A61N 5/02, A61N 5/6). С, in the course of time, determined by the type of tumor, its size, localization and the type of ferromagnetic particles selected for induction heating, while heating is carried out only at the moments of a decrease in tissue blood filling, i.e. at the moments of expiration and diastole of the patient's heart. The heating range is controlled by a microwave deep thermometer, and heating is carried out automatically, with the help of a computer, in the biocontrol mode, according to the algorithms of the mathematical model of fluctuations in the thermal conductivity and heat capacity of the tissue, the hysteresis of heating and heat removal.

The main disadvantages of this method are the low localization of magnetic particles in the tumor and the difficulty of maintaining a fixed temperature in different spatial regions of the tumor, which does not lead to a complete cure of patients.

A known method of destruction of cancerous tumors using magnetic nanoparticles (Presentation of a new magnetic field therapy system for the treatment of human solid tumors with magnetic fluid hyperthermia. Andreas Jordan, Regina Scholz, Klaus Maier-Hau, Manfred Johannsen, Peter Wust, Jacek Nadobny, Hermann Schirra, Helmut Schmidt, SerdarDeger, Stefan Loening, Wolfgang Lanksch, Roland Felix. Journal of Magnetis mand Magnetic Materials 225 (2001) 118-126).

The destruction of cancer cells is based on thermolysis of magnetic nanoparticles injected into the tumor and their induction heating in an alternating magnetic field at frequencies of 50-100 kHz.

However, this method does not allow local destruction of cancer cells and requires powerful electromagnets with currents of tens of kA at relatively high frequencies. In addition, powerful alternating magnetic fields can affect the processes of movement and diffusion of ions through cell membranes, as well as generate inductive alternating electric fields that affect the operation of neural networks in the human body, associated with heating not only magnetic particles, but all cells. located in the area of injection of magnetic particles, and a strong spatial inhomogeneity of the heating temperature both inside the tumor and healthy tissues, damaging them and does not guarantee the complete death of tumor cells.

The known method of close-focus X-ray therapy with a total focal area of 100-120 Gy and remote gamma - therapy for radiation destruction of malignant cells with a total focal area of 30-40 Gy (see Sh.Kh. Gantsev. Oncology, M .: Medical Information Agency. 2004, pp. 190-204; Stephen J., Withrow E., MacEwen G. Smalanimalclinicaloncology - 2001, pp. 305-308).

However, this method, despite its prevalence, has the following disadvantages. In the treatment of some types of malignant neoplasms, for example melanoma, with the help of remote gamma therapy, even in combination with immunotherapy, experience shows that it leads to 75-90% of tumor recurrence, and after 2-6 months metastases occur.

The known method of neuron - capturing selective destruction of melanoma (see V. N. Mitin, N. G. Kozlovskaya, AM Arnopolskaya Neuron-capturing therapy of tumors of the oral cavity in dogs. All-Russian veterinary journal. 2006. No. 1, pp. 9-10) ...

The method involves the intravenous injection of L-borphenylalanine into the blood, which selectively accumulates in a specific tumor - melanoma, since L-phenylalanine is an essential amino acid from which melanin is produced, which forms melanocytes contained in melanoma cells. Thus, there is a selective accumulation of L-borphenylalanine in melanoma cells. When a spatial zone comparable to a tumor containing L-borphenylalanine is irradiated by a beam of slow neurons obtained through a neuron guide from a nuclear reactor, melanoma cells are destroyed due to induced secondary local boron radiation.

However, this method has the following disadvantages:

1. Radiation exposure of patients, which is only partially reduced by using a lithium protective apron.

2. A complex and very expensive installation, including a compact nuclear reactor, requiring qualified non-medical specialists, in particular nuclear physicists, to service.

3. Long-term irradiation of patients within an hour while monitoring the cardiovascular system.

4. Application of general anesthesia.

A known method of photodynamic destruction of tumors, including intravenous administration of a photosensitizer and irradiation of the tumor with continuous laser radiation with a wavelength coinciding with the absorption band of the photosensitizer (see Photodynamictherapy / Ed.TJ Dougherty / J. Clin. LaserMedSurg. 1996, Vol. 14, P 219- 348; RF Patent No. 2184578, IPC A61N 5/06). The selective photodynamic mechanism of destruction of cancer cells is based on a higher density (contrast) of the accumulation of the photosensitizer in tumor cells as compared to healthy cells, which is associated with a high density of blood vessels in a tumor as compared to healthy biological tissue.

However, this contrast for various tumors does not exceed two to three times. When laser radiation is absorbed by a photosensitizer, dye molecules pass into an excited electronic state, and upon collision with oxygen molecules dissolved in biological tissue, they transfer it from an unexcited to an excited electronic singlet state, with a typical lifetime of several microseconds. During this time, singlet oxygen molecules, having traveled a characteristic path commensurate with the size of cells when interacting with the plasma membrane of the cell, damage it, and the cell dies due to necrosis. Thus, the destruction of cells occurs only during exposure to laser radiation in the spatial area of laser irradiation.

The photodynamic method for the destruction of cancer cells has several disadvantages. The photosensitizers used in practice - phthalacionines, porphyrins, chlorins - have absorption bands of photosensitizers in the ultraviolet or visible region of the spectrum, and the lasers used cannot effectively penetrate to a depth of less than a few millimeters. In addition, the photodynamic method has a low contrast of the accumulation of photosensitizers in cancer cells.

The closest to the claimed method is the destruction of biological tissue, which consists in introducing ethanol into it using a hollow game, characterized in that 95% ethanol is introduced in an amount equal to half the volume of biological tissue to be destroyed, then 5 ml of 20-30% ethanol is introduced, after which is carried out by heating with a high-frequency current with the simultaneous introduction of 20-30% ethanol in an amount equal to the volume of biological tissue to be destroyed. The device contains a high-frequency current generator with two cylindrical electrodes located coaxially relative to each other, internal in the form of a hollow needle through which ethanol is injected into the tumor (Abstract No. 2006113533 of the RF patent application). The disadvantages of this method include: the unreasonableness of selective absorption of ethanol by cancer and healthy cells, the difficulty of inserting a coaxial electrode into heterogeneous tumors, to organize uniform heating of tumor tissues not equally located from the exposed end of the needle.

The objective of the present invention is the local selective destruction of malignant tumors deeply located in human biological tissues, preliminarily maximally selectively saturated with succinic acid for 0.5-2.5 hours and 3-aminophthalic acid hydrazide for 6-8 hours, while simultaneously after 8 hours introduction and subsequent saturation of tumors, UC 2.5 hours before selective HF and microwave heating of tumor tissues heated for 199 seconds to a temperature of 51.5 ° C at a heating rate of 0.078 ° C / s, and subsequent heating due to heat transfer of border layers of tumor and to healthy tissues with complete destruction of tumors and border cells of healthy tissues surrounding tumors.

According to the studies carried out on HF and microwave hyperthermia of tumor tissues, at a temperature of 51.5 ° C, the border between the zone of necrosis and healthy tissue is several cells. The zone of destruction of tumor tissue includes a small zone of the periphery of normal healthy tissues, which excludes degenerating cells from metastasis through their secondary necrosis from tumor tissues.

The proposed method for initiating the death of tumor cells with HF and UHF energy, including preliminary administration to a person with solutions of succinic acid 2400 mg and hydrazide 3-aminophthalic acid in a single dose of 35 mg per kilogram of human weight for their maximum accumulation, respectively, for 0.5-2, 5 hours and within 6-8 hours, in tumor cells 3-6 times higher than in healthy ones, characterized in that within 3 days before treatment, a person is transferred to a protein diet, to maximize the multiple selective accumulation in " hungry "tumor tissues with highly electrically conductive electron-ionic solutions of succinic and hydrazide 3-aminophthalic acid, increases the electrical conductivity of tumor tissues in comparison with healthy ones, and after 8 hours after taking the drug" Galavit "and taking UC, 2.5 hours before treatment, selective HF and microwave photoelectromagnetic hyperthermia of tumor tissues with the energy of photowave radiation, with the heating rate of tumor tissues 0.078 ° С / s, during 191 s to a temperature of tumor tissues of 51.5 ° С, when heating healthy tissues no higher than 40 ° С

A one-time megadose of 36 mg / kg of human weight of 3-aminophthalic acid hydrazide of the drug Galavit is injected intravenously and a one-time megadose of 3000 mg of succinic acid is taken orally before HF and microwave treatment of tumor tissues and heating and hyperthermia of tumor tissues saturated with a solution of hydrazide 3- aminophthalic acid of the drug "Galavit" and YK is produced by HF and microwave energy of photowave radiation, in accordance with the frequency, with the depth of penetration of the electromagnetic wave and with the depth of the location of tumor tissues, at the permitted frequencies f = 13.56 MHz-1100 cm, f = 27 MHz-545 cm, f = 40.68 MHz-370 cm, £ = 433.92 MHz-34.5 cm, f = 915 MHz-16.5 cm and f = 2450 MHz-6.1 cm.

The physical nature of microwave radiation is the physical field of moving electric charges in electric and magnetic fields, which are a single electromagnetic field (EMF), characterized by a frequency of oscillation f. The only difference is in the frequency with which electromagnetic oscillations occur at the corresponding wavelength. The biological effect of EMF on a living organism consists in the absorption of energy by biological tissues characterized by biophysical parameters - dielectric constant and conductivity.

The tissues of the human body, due to the high content of water in them, should be considered as dielectrics with losses. With general body irradiation, the EMF energy penetrates to a depth of 0.5 wavelengths. The intensity of exposure, exposure and dielectric loss and conductivity characterize the selective absorption of EMF by different tissues at the same density of EMF radiation.

where, λ is the wavelength;

c is the speed of propagation of an electromagnetic wave,

ƒ - frequency of oscillations of the electromagnetic field.

The frequency with which the electromagnetic field fluctuates significantly affects the penetration depth of the electromagnetic wave into a biological object.

The reason lies in the commensurability with different physical objects. At f = 13.56 MHz, the EMF wavelength λ = 22 m, at f = 40.68 MHz, the EMF wavelength λ = 7.4 m, at f = 433.92 MHz, the EMF wavelength λ = 69 cm, at f = 915 MHz, EMF wavelength λ = 33 cm, and at f = 2450 MHz, EMF wavelength λ = 12.2 cm. (Table 1)

This determines the choice of equipment for local hyperthermia of tumors located at different depths in biological objects.

Tumor tissues saturated with 3-aminophthalic acid hydrazide and succinic acid are 6-8 times higher than its content in healthy tissues, respectively, its electrical conductivity differs so many times, i.e. the ability of tumor tissues to conduct an electric current is due to the presence of an acidic electrolyte in tumors, free charge carriers - electrically charged particles, which, under the influence of an external electric field in the tumor, create a conduction current.

Another important parameter of dielectric and semiconductor materials, which tumors are, are dielectric losses, they serve to determine the electrical power spent on heating dielectrics and semiconductors in an electromagnetic field. In the reference literature, to characterize the ability of a dielectric to absorb the energy of an alternating electric field, the tgδ of the dielectric loss angle and the dielectric constant ε are used. The physical meaning of tan δ consists in the presence of dielectric losses leading to a phase shift between current and voltage, where the angle between them becomes less than 90 ° by the value, quantitative losses of wave energy are proportional to the dielectric losses ε tanδ.

The loss of electrical conductivity in dielectrics with low specific volume resistivity, for example, refers to absolutely chemically pure water. In nature, water is an excellent solvent and dissolves acids well, and therefore the electrical conductivity of such water has a large number of charged ions, which, under the influence of an alternating electric field, begin to move in time with the changing wave electromagnetic field, converting electrical energy into thermal energy. Tumor tissues maximally saturated with 3-aminophthalic acid hydroside and UC, in this case are semiconductors containing several times more charged ions in comparison with surrounding healthy tissues and, accordingly, their heating rate is many times higher than that of surrounding healthy tissues for the same time. In such tumor tissues, relaxation dielectric losses are also observed, due to the rotation of polar water molecules in the direction of the lines of force of the electric field. Intramolecular friction arises, which once again increases the heating of tumor tissues.

The specific power of dielectric losses per unit volume of the dielectric is called dielectric losses, which can be calculated by the formula:

P _beats = E ² f εtgδ, 10 ^-12 W / cm ³

This ratio determines the degree of heating of various structures of tumor and healthy tissues of a biological substance in an electric field. To do this, it is necessary to know the ε and tgδ of the tumor and healthy tissues, and thus very accurately calculate the heating rate to the specified heating temperature of the tumor and surrounding healthy tissues in a uniform electromagnetic field (EMF).

Selective uptake of 3-aminophthalic acid hydrazide and UC by tumor tissues leads to their selective heating of tumors and electromagnetic photoluminescence to a higher temperature of 50 ° С when heating the surrounding healthy tissues to 40 ° С during the same time, which leads to inoculation of tumor tissues and their subsequent destruction, which then, within a few days, are painlessly excreted by the body. The rate of heating by the wave energy of the electromagnetic field depends on the power of the dielectric generators and magnetrons.

With the oscillatory power of the generators of the electromagnetic field of 700-850 watts, 200-300 grams of tumor tissues can be heated to a temperature of 60 ° C in 2-3 minutes, the specific power released in the tumors and their heating temperature is determined by the formula:

where, Co - heat capacity of the tumor, feces;

m is the mass of the tumor in grams;

ΔТ - heating temperature difference;

t - heating time, s.

This formula allows you to select the required total specific power R _beats. for HF and microwave heating of tumor tissues P _beats. to a given difference in heating temperatures and specific power R _beats. released in healthy tissues, determined by the general formula:

R _ud.ob. = R _beats. + R _ud.zd.

Then the specific power in the field of irradiation, taking into account the dielectric properties:

R _ud.ob. = (E ² f ε tgδ _{op op} + E ² f ε _{zd zd} tgδ) 10 ^-12

Knowing the dielectric properties ε tumor tgδ _{op op} and healthy tkaney ε _zd tgδ _zd can determine by calculation the temperature of the heating? T to the required predetermined temperature and to determine the heating time t and the total power density P _ud.ob. , the irradiated area. (Table 2)

Similarly, knowing the dielectric parameters εtgδ and the specific density of tumor tissues of saturated electrophotosensitizers in biological objects γ g / cm ³ , it is possible by calculation to find the specific power released in the tumor tissues P _{beats op} , and determine the set temperature and calculate the time of their heating by HF and microwave -energy, according to the above formulas.

Claims (1)

A method for initiating the death of tumor cells with HF and UHF energy, intended for the local treatment of cancer patients with tumor tissues, for their hyperthermia in all organs of the human body, characterized by the fact that before treatment, a person is transferred to a protein diet for 3 days for maximum multiple selective increase in the accumulation in "hungry" tumor tissues of highly electrically conductive electron-ionic solution of hydrazide 3-aminophthalic acid when administered intravenously at a megadose of 36 mg / kg of human weight over 8 hours and oral administration of succinic acid in a single megadose of 3000 mg 2.5 hours before the start physiotherapy with the maximum accumulation during this time in tumor tissues of the drug "Galavit", 3-6 times higher than in healthy tissues, selective hyperthermia of tumor tissues is carried out with HF energy in accordance with the depth of their location and the depth of penetration of an electromagnetic wave into the human body for 6.1 centimeters at the permitted frequency f = 2450 MHz, with a total heating rate of tumor tissues at this frequency of 0.078 ° C / s, for 191 s to a heating temperature of tumor tissues of 51.5 ° C, while heating healthy tissues no higher than 40 ° C.