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SG11201804401VA - Determination of nucleic acid methylation - Google Patents

Determination of nucleic acid methylation

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Publication number
SG11201804401VA
SG11201804401VA SG11201804401VA SG11201804401VA SG11201804401VA SG 11201804401V A SG11201804401V A SG 11201804401VA SG 11201804401V A SG11201804401V A SG 11201804401VA SG 11201804401V A SG11201804401V A SG 11201804401VA SG 11201804401V A SG11201804401V A SG 11201804401VA
Authority
SG
Singapore
Prior art keywords
international
methylation
nucleic acid
target
singapore
Prior art date
Application number
SG11201804401VA
Inventor
Yanbing Zu
Jackie Y Ying
Original Assignee
Agency Science Tech & Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency Science Tech & Res filed Critical Agency Science Tech & Res
Publication of SG11201804401VA publication Critical patent/SG11201804401VA/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/10—Characterised by chemical treatment
    • C12Q2523/125—Bisulfite(s)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/113—Modifications characterised by incorporating modified backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/131—Allele specific probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/163—Reactions characterised by the reaction format or use of a specific feature the purpose or use of blocking probe
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/155—Particles of a defined size, e.g. nanoparticles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/107—Alteration in the property of hybridised versus free label oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/154—Methylation markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Nanotechnology (AREA)
  • General Chemical & Material Sciences (AREA)

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property ' Organization International Bureau res.. 00) (43) International Publication Date .... ...r .„„*./ 1 June 2017 (01.06.2017) WIPO I PCT (10) WO International 111111111111311111111111111111111111111111111111111111111111111111111311111111111111111 2017/091156 Publication Number Al (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every C12Q 1/68 (2006.01) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (21) International Application Number: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, PCT/SG2016/050583 DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (22) International Filing Date: HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, 25 November 2016 (25.11.2016) KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (25) Filing Language: English OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (26) Publication Language: English SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, (30) Priority Data: ZW. 10201509792S 27 November 2015 (27.11.2015) SG (84) Designated States (unless otherwise indicated, for every (71) Applicant: AGENCY FOR SCIENCE, TECHNOLOGY kind of regional protection available): ARIPO (BW, GH, AND RESEARCH [SG/SG]; 1 Fusionopolis Way, #20-10 GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, Connexis, Singapore 138632 (SG). TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, (72) Inventors: ZU, Yanbing; do Institute of Bioengineering TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, and Nanotechnology, 31 Biopolis Way, The Nanos, #04- 01, Singapore 138669 (SG). YING, Jackie Y.; c/o Institute LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, of Bioengineering and Nanotechnology, 31 Biopolis Way, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). The Nanos, #04-01, Singapore 138669 (SG). Published: (74) Agent: VIERING, JENTSCHURA & PARTNER LLP; 21(3)) P.O. Box 1088, Rochor Post Office, Rochor Road, Singa- — with international search report (Art pore 911833 (SG). — with sequence listing part of description (Rule 5.2(a)) (54) Title: DETERMINATION OF NUCLEIC ACID METHYLATION Figure 1 \ lix yi s 'Iml kelp D 11 A.mt3lify .. `iEPT., \ Read A co, i Isasst s Lit A Jn AS 6.Ieekee p:pm:Pter PCP Futiuct Various Promega Modified Asymmetric IEN nanoprobe Color signals o commercial MethylEdgeT\" sequences PCR methods Bisulfite according to Conversion references ( - MS methylation-specific) System - 2 5 h t 2. , t mitt V: in 1 Li min IS :. <,‘,.. ‘ CIr.\" If , I :\"' \".: \" ' 3,wkau*nmlis-, 11 os Cr\ i3,:n.st(sft {flati tleikbti.,${ .\.... L ....' (57) : The invention relates to methods and ..::::; sample comprising (a) bisulfite treatment of the target el tion-specific primers, (c) contacting the amplicons Oation status based on melting temperature T„, of the ,-t particle covalently coupled to a morpholino oligonucleotide . kits for determining the methylation nucleic acid, (b) amplifying with a methylation-specific plasmonic hybrid probe and the target probe. Also claimed - the nucleic are methods Mr.;$ 141a/ m, status of a target treated target nucleic nanoprobe and (d) acid. In particular, and kits for nucleic acid molecule in a acid with methyla - deteiinining the methyl - a plasmonic gold nano - determining methylation status of the Septin 9 (SEPT9) gene promoter.
SG11201804401VA 2015-11-27 2016-11-25 Determination of nucleic acid methylation SG11201804401VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SG10201509792S 2015-11-27
PCT/SG2016/050583 WO2017091156A1 (en) 2015-11-27 2016-11-25 Determination of nucleic acid methylation

Publications (1)

Publication Number Publication Date
SG11201804401VA true SG11201804401VA (en) 2018-06-28

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SG11201804401VA SG11201804401VA (en) 2015-11-27 2016-11-25 Determination of nucleic acid methylation

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US (2) US11021743B2 (en)
EP (1) EP3380637B1 (en)
CN (1) CN108495937A (en)
SG (2) SG10201912967YA (en)
WO (1) WO2017091156A1 (en)

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Publication number Priority date Publication date Assignee Title
JP7245164B2 (en) * 2017-08-31 2023-03-23 公益財団法人がん研究会 Highly sensitive and quantitative genetic testing method, primer set, and test kit
CN108796080B (en) * 2018-06-06 2022-06-07 苏州唯善生物科技有限公司 Primer and probe set for colorectal cancer diagnosis, detection or screening
CN109929919B (en) * 2018-09-14 2020-02-14 深圳市晋百慧生物有限公司 DNA methylation detection method and related application
CN115786517A (en) * 2019-02-14 2023-03-14 博尔诚（北京）科技有限公司 Composition for screening cancers of intestinal cancers and precancers and application of composition
CN109765371A (en) * 2019-02-19 2019-05-17 吉林省爱诺德生物工程有限公司 A kind of liquid phase nano colloid gold colorimetric method of quick detection neoplasm dna
WO2021122799A1 (en) * 2019-12-16 2021-06-24 Epigenomics Ag Methods for detecting colorectal cancer
WO2021250135A1 (en) * 2020-06-09 2021-12-16 Indicate Solutions, S.L. Nanoparticle probes and their use in nucleic acid detection
CN112195243A (en) * 2020-09-22 2021-01-08 北京华大吉比爱生物技术有限公司 Kit for detecting polygene methylation and application thereof
CN116949156B (en) * 2023-09-19 2023-12-08 美迪西普亚医药科技(上海)有限公司 Analysis method for detecting human T cells in general way based on nucleic acid variants

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US5472881A (en) 1992-11-12 1995-12-05 University Of Utah Research Foundation Thiol labeling of DNA for attachment to gold surfaces
WO2003048769A1 (en) * 2001-11-30 2003-06-12 Nanosphere, Inc. Real-time monitoring of pcr amplification using nanoparticle probes
US20080241828A1 (en) * 2007-03-30 2008-10-02 Kai Wu Detection of dna methylation using raman spectroscopy
ES2769841T3 (en) * 2007-06-08 2020-06-29 Epigenomics Ag Methylation analysis method
EP2189538A1 (en) * 2008-11-21 2010-05-26 Epigenomics AG An analysis molecule and method for the analysis of a nucleotide position in a nucleic acid
CN102884204B (en) * 2010-01-12 2015-03-25 新加坡科技研究局 Sequence-selective recognition of nucleic acids using nanoparticle probes
US9365902B2 (en) * 2013-03-15 2016-06-14 Abbott Molecular Inc. Detection of bisulfite converted nucleotide sequences
SG10201708046YA (en) * 2013-04-11 2017-10-30 Agency Science Tech & Res Nanoprobe-Based Genetic Testing
US10041121B2 (en) * 2013-09-17 2018-08-07 Agency For Science, Technology And Research Method for detection of a genetic variant
CN103789429B (en) * 2014-01-24 2015-12-30 武汉大学 A kind of method of the 5-methylcytosine detected in DNA
KR101612885B1 (en) * 2014-03-27 2016-04-26 고려대학교 산학협력단 Methods for Detection Methylation on Promoter and Methods for Detection and Tracking Epigenetics Alterations by DNA methylation Using Nanoplasmonic Biosensor

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Publication number Publication date
EP3380637A1 (en) 2018-10-03
US11021743B2 (en) 2021-06-01
SG10201912967YA (en) 2020-02-27
EP3380637A4 (en) 2019-05-01
CN108495937A (en) 2018-09-04
US20210222237A1 (en) 2021-07-22
US20190017110A1 (en) 2019-01-17
EP3380637B1 (en) 2020-09-30
WO2017091156A1 (en) 2017-06-01

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