SG11201804082XA - Improved protein separation in ion exchange chromatography - Google Patents
Improved protein separation in ion exchange chromatographyInfo
- Publication number
- SG11201804082XA SG11201804082XA SG11201804082XA SG11201804082XA SG11201804082XA SG 11201804082X A SG11201804082X A SG 11201804082XA SG 11201804082X A SG11201804082X A SG 11201804082XA SG 11201804082X A SG11201804082X A SG 11201804082XA SG 11201804082X A SG11201804082X A SG 11201804082XA
- Authority
- SG
- Singapore
- Prior art keywords
- international
- pct
- preparative
- protein separation
- ion exchange
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 5
- 108090000623 proteins and genes Proteins 0.000 title abstract 5
- 238000000926 separation method Methods 0.000 title abstract 5
- 238000004255 ion exchange chromatography Methods 0.000 title abstract 2
- 238000010828 elution Methods 0.000 abstract 1
- 230000008520 organization Effects 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/361—Ion-exchange
- B01D15/362—Cation-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
- B01D15/361—Ion-exchange
- B01D15/363—Anion-exchange
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/52—Constant or Fc region; Isotype
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/55—Fab or Fab'
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Peptides Or Proteins (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property - Organization International Bureau (43) International Publication Date .... ....r .....1 26 May 2017(26.05.2017) WIPO I PCT (10) WO International 111111111111311111111111111111111111111111111111111111111111111111111111111111111111111111 2017/084737 Publication Number Al (51) International Patent Classification: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, CO7K 1/16 (2006.01) C07K 16/00 (2006.01) DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, CO7K 1/18 (2006.01) HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, (21) International Application Number: MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, PCT/EP2016/001803 OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (22) International Filing Date: SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, 28 October 2016 (28.10.2016) TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (25) Filing Language: English (84) Designated States (unless otherwise indicated, for every (26) Publication Language: English kind of regional protection available): ARIPO (BW, GH, (30) Priority Data: GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, 15195242.1 18 November 2015 (18.11.2015) EP TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, (71) Applicant: MERCK PATENT GMBH [DE/DE]; Frank- DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, furter Strasse 250, 64293 Darmstadt (DE). LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, (72) Inventor: JOEHNCK, Matthias; Lindenbergweg 3, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, 64367 Muehltal (DE). GW, KM, ML, MR, NE, SN, TD, TG). (81) Designated States (unless otherwise indicated, for every Published: kind of national protection available): AE, AG, AL, AM, — with international search report (Art 21(3)) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, 1-1 .4t IN M IN i t Ge C::: , IN 11 0 (54) Title: IMPROVED PROTEIN SEPARATION IN ION EXCHANGE CHROMATOGRAPHY ei 0 (57) : The present invention relates to improved preparative (>5 g/1) protein separations. These improvements are achieved by combining salt and pH gradients for preparative protein separations in combination with the development of a preparative step elution protein separation based on data generated by combined salt-pH gradient runs.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP15195242 | 2015-11-18 | ||
| PCT/EP2016/001803 WO2017084737A1 (en) | 2015-11-18 | 2016-10-28 | Improved protein separation in ion exchange chromatography |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201804082XA true SG11201804082XA (en) | 2018-06-28 |
Family
ID=54608366
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201804082XA SG11201804082XA (en) | 2015-11-18 | 2016-10-28 | Improved protein separation in ion exchange chromatography |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US20180327447A1 (en) |
| EP (1) | EP3377513A1 (en) |
| JP (1) | JP2018538268A (en) |
| KR (1) | KR20180083409A (en) |
| CN (1) | CN108350026A (en) |
| AU (1) | AU2016355739A1 (en) |
| BR (1) | BR112018009881A2 (en) |
| CA (1) | CA3005483A1 (en) |
| IL (1) | IL259180A (en) |
| MX (1) | MX2018005834A (en) |
| RU (1) | RU2018121653A (en) |
| SG (1) | SG11201804082XA (en) |
| WO (1) | WO2017084737A1 (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3876996A1 (en) * | 2018-11-05 | 2021-09-15 | Bristol-Myers Squibb Company | Method for purifying pegylated protein |
| IL294901A (en) * | 2020-01-24 | 2022-09-01 | Alkermes Pharma Ireland Ltd | Methods for purification |
| WO2021210662A1 (en) * | 2020-04-17 | 2021-10-21 | 小野薬品工業株式会社 | Color removal method for protein formulation active ingredient |
| US20230167153A1 (en) * | 2020-05-01 | 2023-06-01 | Kashiv Biosciences, Llc | An improved process of purification of protein |
| WO2022239704A1 (en) * | 2021-05-10 | 2022-11-17 | 株式会社カイオム・バイオサイエンス | Purification method of antibody composition |
| WO2024261724A1 (en) * | 2023-06-23 | 2024-12-26 | Lupin Limited | Ion-exchange chromatography for separation and analysis of charge variants |
| US20250251376A1 (en) * | 2024-01-26 | 2025-08-07 | Mriglobal | Systems and methods for separation of crispr/cas components |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE321066T1 (en) * | 1998-05-06 | 2006-04-15 | Genentech Inc | ANTI-HER2 ANTIBODY COMPOSITION |
| CN1234725C (en) * | 2004-04-07 | 2006-01-04 | 陈志南 | High performance quick purifying method for preparing piecewise antibody |
| TWI320788B (en) * | 2005-05-25 | 2010-02-21 | Hoffmann La Roche | Method for the purification of antibodies |
| BR112012024956A2 (en) * | 2010-03-30 | 2016-07-12 | Octapharma Ag | process for purifying a growth factor protein |
| SG10201701224UA (en) * | 2012-03-12 | 2017-04-27 | Merck Patent Gmbh | Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode |
| US9150645B2 (en) * | 2012-04-20 | 2015-10-06 | Abbvie, Inc. | Cell culture methods to reduce acidic species |
| KR102133699B1 (en) * | 2012-09-11 | 2020-07-14 | 코히러스 바이오사이언시즈, 인코포레이티드 | Correctly folded etanercept in high purity and excellent yield |
| BR112015011011A2 (en) * | 2012-11-15 | 2019-12-17 | Genentech Inc | ion-strength ion-gradient ion exchange chromatography |
| CN104628846B (en) * | 2013-11-06 | 2019-12-06 | 三生国健药业（上海）股份有限公司 | Method for purifying recombinant protein |
| DK2905289T3 (en) * | 2014-02-11 | 2017-01-30 | Richter-Helm Bio Tec Gmbh & Co Kg | Method of Purifying Teriparatide (PTH1-34) |
| EP3377514A1 (en) * | 2015-11-18 | 2018-09-26 | Merck Patent GmbH | Opposite ph-salt gradients for improved protein separations |
-
2016
- 2016-10-28 CN CN201680067565.9A patent/CN108350026A/en active Pending
- 2016-10-28 JP JP2018526200A patent/JP2018538268A/en active Pending
- 2016-10-28 WO PCT/EP2016/001803 patent/WO2017084737A1/en active Application Filing
- 2016-10-28 KR KR1020187017147A patent/KR20180083409A/en not_active Withdrawn
- 2016-10-28 AU AU2016355739A patent/AU2016355739A1/en not_active Abandoned
- 2016-10-28 RU RU2018121653A patent/RU2018121653A/en not_active Application Discontinuation
- 2016-10-28 BR BR112018009881A patent/BR112018009881A2/en not_active Application Discontinuation
- 2016-10-28 MX MX2018005834A patent/MX2018005834A/en unknown
- 2016-10-28 SG SG11201804082XA patent/SG11201804082XA/en unknown
- 2016-10-28 CA CA3005483A patent/CA3005483A1/en not_active Abandoned
- 2016-10-28 EP EP16788429.5A patent/EP3377513A1/en not_active Withdrawn
- 2016-10-28 US US15/776,941 patent/US20180327447A1/en not_active Abandoned
-
2018
- 2018-05-07 IL IL259180A patent/IL259180A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| RU2018121653A3 (en) | 2020-01-31 |
| EP3377513A1 (en) | 2018-09-26 |
| JP2018538268A (en) | 2018-12-27 |
| CN108350026A (en) | 2018-07-31 |
| BR112018009881A2 (en) | 2018-11-13 |
| AU2016355739A1 (en) | 2018-07-05 |
| MX2018005834A (en) | 2018-08-01 |
| KR20180083409A (en) | 2018-07-20 |
| WO2017084737A1 (en) | 2017-05-26 |
| RU2018121653A (en) | 2019-12-19 |
| IL259180A (en) | 2018-07-31 |
| CA3005483A1 (en) | 2017-05-26 |
| US20180327447A1 (en) | 2018-11-15 |
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